Starting /dee2/code/volunteer_pipeline.sh SRR6322411 current disk space = 1543543660544 free memory = 1597732716 SRR6322411 SRAfilesize b8ffb75ddc43e350c1dbac0b70cf689f SRR6322411.sra SRR6322411.sra file validated SRR6322411 is single end SRR6322411 is conventional basespace SRR6322411 read1 length is 50 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR6322411_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 50 %GC 54 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.01975 33.0 33.0 34.0 31.0 34.0 2 32.3985 33.0 33.0 34.0 30.0 34.0 3 32.403 33.0 33.0 34.0 31.0 34.0 4 32.2555 33.0 33.0 34.0 31.0 34.0 5 32.317 33.0 33.0 34.0 31.0 34.0 6 35.73925 38.0 36.0 38.0 30.0 38.0 7 36.14 38.0 37.0 38.0 33.0 38.0 8 36.4015 38.0 37.0 38.0 34.0 38.0 9 36.43025 38.0 38.0 38.0 33.0 38.0 10 36.579 38.0 38.0 38.0 34.0 38.0 11 36.508 38.0 38.0 38.0 34.0 38.0 12 36.58825 38.0 38.0 38.0 34.0 38.0 13 36.51375 38.0 38.0 38.0 34.0 38.0 14 36.63675 38.0 38.0 38.0 34.0 38.0 15 36.68575 38.0 38.0 38.0 34.0 38.0 16 36.6285 38.0 38.0 38.0 34.0 38.0 17 36.5955 38.0 38.0 38.0 34.0 38.0 18 36.5325 38.0 38.0 38.0 34.0 38.0 19 36.57825 38.0 38.0 38.0 34.0 38.0 20 36.40275 38.0 38.0 38.0 34.0 38.0 21 36.528 38.0 38.0 38.0 34.0 38.0 22 36.4475 38.0 38.0 38.0 34.0 38.0 23 36.55225 38.0 38.0 38.0 34.0 38.0 24 36.5655 38.0 38.0 38.0 34.0 38.0 25 36.56075 38.0 38.0 38.0 34.0 38.0 26 36.52275 38.0 38.0 38.0 34.0 38.0 27 36.44775 38.0 38.0 38.0 34.0 38.0 28 36.4885 38.0 38.0 38.0 34.0 38.0 29 36.436 38.0 38.0 38.0 34.0 38.0 30 36.4815 38.0 38.0 38.0 34.0 38.0 31 36.56725 38.0 38.0 38.0 34.0 38.0 32 36.5455 38.0 38.0 38.0 34.0 38.0 33 36.3815 38.0 38.0 38.0 34.0 38.0 34 36.40075 38.0 38.0 38.0 34.0 38.0 35 36.268 38.0 38.0 38.0 33.0 38.0 36 36.34275 38.0 38.0 38.0 33.0 38.0 37 36.361 38.0 38.0 38.0 34.0 38.0 38 36.3105 38.0 38.0 38.0 33.0 38.0 39 36.35075 38.0 38.0 38.0 33.0 38.0 40 36.24125 38.0 38.0 38.0 33.0 38.0 41 36.36975 38.0 38.0 38.0 34.0 38.0 42 36.25425 38.0 38.0 38.0 33.0 38.0 43 36.14325 38.0 38.0 38.0 33.0 38.0 44 36.31175 38.0 38.0 38.0 33.0 38.0 45 36.151 38.0 38.0 38.0 33.0 38.0 46 36.1105 38.0 38.0 38.0 33.0 38.0 47 36.21825 38.0 38.0 38.0 33.0 38.0 48 36.1505 38.0 38.0 38.0 33.0 38.0 49 35.93825 38.0 38.0 38.0 33.0 38.0 50 35.90275 38.0 37.0 38.0 33.0 38.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0 1101 2 0.0 1101 3 0.0 1101 4 0.0 1101 5 0.0 1101 6 0.0 1101 7 0.0 1101 8 0.0 1101 9 0.0 1101 10 0.0 1101 11 0.0 1101 12 0.0 1101 13 0.0 1101 14 0.0 1101 15 0.0 1101 16 0.0 1101 17 0.0 1101 18 0.0 1101 19 0.0 1101 20 0.0 1101 21 0.0 1101 22 0.0 1101 23 0.0 1101 24 0.0 1101 25 0.0 1101 26 0.0 1101 27 0.0 1101 28 0.0 1101 29 0.0 1101 30 0.0 1101 31 0.0 1101 32 0.0 1101 33 0.0 1101 34 0.0 1101 35 0.0 1101 36 0.0 1101 37 0.0 1101 38 0.0 1101 39 0.0 1101 40 0.0 1101 41 0.0 1101 42 0.0 1101 43 0.0 1101 44 0.0 1101 45 0.0 1101 46 0.0 1101 47 0.0 1101 48 0.0 1101 49 0.0 1101 50 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6.0 3 1.0 4 0.0 5 2.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 1.0 12 0.0 13 0.0 14 0.0 15 0.0 16 2.0 17 0.0 18 1.0 19 1.0 20 0.0 21 3.0 22 2.0 23 3.0 24 9.0 25 9.0 26 25.0 27 29.0 28 41.0 29 53.0 30 64.0 31 102.0 32 124.0 33 140.0 34 182.0 35 273.0 36 627.0 37 2300.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 38.656527249683144 12.344740177439798 8.770595690747781 40.22813688212928 2 27.275 17.224999999999998 36.449999999999996 19.05 3 24.75 20.0 23.724999999999998 31.525 4 30.45 26.3 19.55 23.7 5 28.825 27.200000000000003 21.125 22.85 6 23.575 26.650000000000002 22.35 27.425 7 21.95 14.025000000000002 39.725 24.3 8 25.224999999999998 15.125 25.2 34.449999999999996 9 22.475 14.75 30.975 31.8 10 27.125 27.650000000000002 21.8 23.425 11 30.975 15.725 18.85 34.449999999999996 12 29.599999999999998 15.625 24.85 29.925 13 26.275 19.35 25.650000000000002 28.725 14 25.374999999999996 21.75 23.925 28.95 15 26.125 21.099999999999998 26.200000000000003 26.575 16 26.650000000000002 20.474999999999998 24.75 28.125 17 27.075 21.349999999999998 25.924999999999997 25.650000000000002 18 26.150000000000002 21.725 24.525 27.6 19 27.250000000000004 21.625 23.400000000000002 27.725 20 28.349999999999998 22.275 24.95 24.425 21 25.650000000000002 22.325 24.8 27.224999999999998 22 26.875 22.475 23.724999999999998 26.924999999999997 23 25.924999999999997 21.475 25.674999999999997 26.924999999999997 24 25.525 19.475 27.900000000000002 27.1 25 25.25 20.9 25.724999999999998 28.125 26 27.375 21.025 25.124999999999996 26.474999999999998 27 25.575 21.525 27.0 25.900000000000002 28 27.025 21.224999999999998 24.375 27.375 29 25.525 21.6 25.95 26.924999999999997 30 26.0 21.375 26.200000000000003 26.424999999999997 31 27.7569392348087 21.305326331582897 23.655913978494624 27.28182045511378 32 26.224999999999998 21.275 26.450000000000003 26.05 33 27.25681420355089 19.954988747186796 24.85621405351338 27.93198299574894 34 25.174999999999997 21.175 26.275 27.375 35 26.625 20.75 25.575 27.05 36 25.4 21.175 24.925 28.499999999999996 37 26.950000000000003 20.7 25.474999999999998 26.875 38 26.424999999999997 21.925 24.575 27.075 39 26.900000000000002 21.15 24.15 27.800000000000004 40 28.382095523880967 20.980245061265315 24.33108277069267 26.30657664416104 41 24.95 22.175 23.775 29.099999999999998 42 25.35 21.875 25.3 27.474999999999998 43 26.05 22.225 24.325 27.400000000000002 44 26.6 21.025 25.974999999999998 26.400000000000002 45 25.95 21.775 24.725 27.55 46 25.35 20.45 25.674999999999997 28.525 47 24.875 21.0 26.575 27.55 48 25.6 22.05 24.474999999999998 27.875 49 25.5 22.175 24.575 27.750000000000004 50 27.575 21.0 24.25 27.175 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 1.0 22 2.0 23 3.5 24 5.0 25 3.5 26 2.0 27 5.0 28 8.0 29 10.5 30 13.0 31 20.5 32 28.0 33 41.0 34 54.0 35 66.0 36 78.0 37 85.0 38 92.0 39 117.0 40 142.0 41 174.0 42 206.0 43 221.5 44 237.0 45 240.5 46 244.0 47 262.0 48 280.0 49 273.5 50 267.0 51 276.5 52 286.0 53 268.0 54 250.0 55 246.5 56 243.0 57 234.0 58 225.0 59 230.5 60 236.0 61 218.5 62 201.0 63 183.0 64 165.0 65 178.0 66 191.0 67 179.5 68 168.0 69 142.5 70 117.0 71 95.5 72 74.0 73 74.5 74 75.0 75 61.5 76 48.0 77 36.5 78 25.0 79 23.5 80 22.0 81 16.0 82 10.0 83 7.0 84 4.0 85 2.5 86 1.0 87 1.0 88 1.0 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 1.375 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.025 32 0.0 33 0.025 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.025 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 50 4000.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 79.825 #Duplication Level Percentage of deduplicated Percentage of total 1 86.31381146257439 68.89999999999999 2 8.518634512997181 13.600000000000001 3 2.2862511744440965 5.475 4 1.4719699342311305 4.7 5 0.6263701847792045 2.5 6 0.3758221108675227 1.7999999999999998 7 0.1252740369558409 0.7000000000000001 8 0.03131850923896023 0.2 9 0.06263701847792046 0.44999999999999996 >10 0.18791105543376135 1.675 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CTCGAGGCTCGATAACACGACGTATGACAGATACGCCGGCACAACGAAAT 14 0.35000000000000003 No Hit GGCGGCTGCTGGCATGATTGTTCCTGTGACGGCTGAAAAGATCTAGTGAC 13 0.325 No Hit CGGGGGTGTTGTGGTATCGAGGTAATAAAAGGCCTCGAGGCTCGATAACA 10 0.25 No Hit GTCGGGTACTGCGGTCCTTGCGGCAACGAGGACGGGTAGTGGGGTCCCTG 10 0.25 No Hit GTTGCTGTTGCTGCTGCTGTTGCTGCTGCTGCTGCTGCTGCATGATGGCC 10 0.25 No Hit GGGAAGTTGACCTGCTGCAGCAGCTTCTTGCCGTCGGTTTCCGAGGCCAC 10 0.25 No Hit GCGAGGTGTAGGCGTGGGTGGTGGGGTTGAAGCTCACGTCCGTCACCAGC 9 0.22499999999999998 No Hit GGCACCTGTTCTGCTGCACTGTAGGCCACGGTCCTAGGATTGACGTCGTC 9 0.22499999999999998 No Hit CGAGGCTCGATAACACGACGTATGACAGATACGCCGGCACAACGAAATTG 8 0.2 No Hit CTTCACTCTCCGCCTTCTTTAAAACGACATAGTTTTGTGGTATGATTAAC 7 0.17500000000000002 No Hit CTCGATTGTCCTTCCTGAGGTTCGATTGGTAGGAAGGGTTGTTTCTGCTG 7 0.17500000000000002 No Hit GTTGAGGCGTGCGATACTGCCAGCACGTGGGTTGTATGTGTCGGCACGGT 7 0.17500000000000002 No Hit CAACTGTTGGCAGCATTGTTGCTGCTTCACCTGGCACCTGTTCTGCTGCA 7 0.17500000000000002 No Hit CCCTGCTGCAGTTGTCTCTCATCTTGTTGTTGATGTGGGCACGGCTGCTC 6 0.15 No Hit GGGCACGGCTGCTCCGGCTGAACCGACCATGGTTTCTCCTGCGCCATGCC 6 0.15 No Hit GGGGTGTTGTGGTATCGAGGTAATAAAAGGCCTCGAGGCTCGATAACACG 6 0.15 No Hit CTCGATAACACGACGTATGACAGATACGCCGGCACAACGAAATTGCTCAT 6 0.15 No Hit CTCGAGGCTCGATAACACGACGTATGACAAATACGCCAGCACAACGAAAT 6 0.15 No Hit CAGGGACGTAGTCAACGCGAGCTGATGACTCGCGCTTACTAGGCATTCCT 6 0.15 No Hit GTGGACTCTCTGGTGCACATCTCCCAACTTTTGGCTTTGGCTTTGGCATT 6 0.15 No Hit CGGGGATGTCGCAGGCTTCGCCCTTGTTGCACATGGAAGGAAGGTTCTTC 6 0.15 No Hit GTTGTTGTTGCTGCTTGATGATGGCATCCACAACGTCCCGGATGGCCTGG 6 0.15 No Hit GTTGTGGCGGCTGCTGGCATGATTGTTCCTGTGACGGCTGAAAAGATCTA 6 0.15 No Hit CACCTGTTCTGCTGCACTGTAGGCCACGGTCCTAGGATTGACGTCGTCTG 6 0.15 No Hit GTCAGACCAGCTTGTGACCTCACTTGCGTAAGTGGTTCAATTGTTTGCAG 6 0.15 No Hit AAAAGGCCTCGAGGCTCGATAACACGACGTATGACAGATACGCCGGCACA 5 0.125 No Hit CTTTTTGGTGCTCATCACTAAATTGTTGGCTTTGGCTTGGACCTTGTGCT 5 0.125 No Hit ATCACGTACACCACGCTATGGGCATTAATGTTCCAGAATGGTGAAACAAT 5 0.125 No Hit GTGGTATCGAGGTAATAAAAGGCCTCGAGGCTCGATAACACGACGTATGA 5 0.125 No Hit GTTGAAACTGCTCCAGGAAAGTCTCTGGGCACCCAGGAAAAGCCAGTCCT 5 0.125 No Hit GTGGCGGCTGCTGGCATGATTGTTCCTGTGACGGCTGAAAAGATCTAGTG 5 0.125 No Hit GACGTCGTCTGCATCTCCGTCGGGCTGCACCGCTGCAGGAAATACTCCCT 5 0.125 No Hit CGTCAGACCAGCTTGTGACCTCACTTGCGTAAGTGGTTCAATTGTTTGCA 5 0.125 No Hit GGCTGATCAGCGGCGGTGGCGCGGCAGTACGGTGGGAGGTACACATTGCA 5 0.125 No Hit GGGACGTAGTCAACGCGAGCTGATGACTCGCGCTTACTAGGCATTCCTCG 5 0.125 No Hit CTCTCTGGTGCACATCTCCCAACTTTTGGCTTTGGCTTTGGCATTGGCTT 5 0.125 No Hit GCCCCTGCTGCAGTTGTCTCTCATCTTGTTGTTGATGTGGGCACGGCTGC 5 0.125 No Hit GGGGTCCCTGCGGCTGCGAGGTCGGGTAGTGTGGTGCTAGCTGGTGCTGA 5 0.125 No Hit AAGGCCTCGAGGCTCGATAACACGACGTATGACAGATACGCCGGCACAAC 5 0.125 No Hit CTTCACGCTCTGCCTTTTTTAGAACAACATAGTTTTGTGGGATGATTAAT 5 0.125 No Hit CGAGTGGCGAGGTGTAGGCGTGGGTGGTGGGGTTGAAGCTCACGTCCGTC 5 0.125 No Hit CTCTGGTGCACATCTCCCAACTTTTGGCTTTGGCTTTGGCATTGGCTTTG 5 0.125 No Hit GTTGCCCCGAACGAAGAAGGCCATTGAACACATTTTGACCCTGGTTGTTG 5 0.125 No Hit GTGAAACAATCGCATTCTTCTGGAGATTTACCCTCGTAGCACTCATTTGC 5 0.125 No Hit CTTGGACCTTGTGCTTGCTCAAATTGTTGGAAGGTCTCTGGGCATCCAGG 5 0.125 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.025 0.0 0.0 0.0 0.0 7 0.025 0.0 0.0 0.0 0.0 8 0.025 0.0 0.0 0.0 0.0 9 0.025 0.0 0.0 0.0 0.0 10 0.025 0.0 0.0 0.0 0.0 11 0.025 0.0 0.0 0.0 0.0 12 0.025 0.0 0.0 0.0 0.0 13 0.025 0.0 0.0 0.0 0.0 14 0.025 0.0 0.0 0.0 0.0 15 0.025 0.0 0.0 0.0 0.0 16 0.025 0.0 0.0 0.0 0.0 17 0.025 0.0 0.0 0.0 0.0 18 0.025 0.0 0.0 0.0 0.0 19 0.025 0.0 0.0 0.0 0.0 20 0.025 0.0 0.0 0.0 0.0 21 0.025 0.0 0.0 0.0 0.0 22 0.025 0.0 0.0 0.0 0.0 23 0.025 0.0 0.0 0.0 0.0 24 0.05 0.0 0.0 0.0 0.0 25 0.05 0.0 0.0 0.0 0.0 26 0.05 0.0 0.0 0.0 0.0 27 0.05 0.0 0.0 0.0 0.0 28 0.05 0.0 0.0 0.0 0.0 29 0.05 0.0 0.0 0.0 0.0 30 0.05 0.0 0.0 0.0 0.0 31 0.05 0.0 0.0 0.0 0.0 32 0.05 0.0 0.0 0.0 0.0 33 0.05 0.0 0.0 0.0 0.0 34 0.05 0.0 0.0 0.0 0.0 35 0.05 0.0 0.0 0.0 0.0 36 0.05 0.0 0.0 0.0 0.0 37 0.05 0.0 0.0 0.0 0.0 38 0.05 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content pass >>END_MODULE Read 1822289 spots for SRR6322411.sra Written 1822289 spots for SRR6322411.sra Read 1822289 spots for SRR6322411.sra Written 1822289 spots for SRR6322411.sra Read 1822289 spots for SRR6322411.sra Written 1822289 spots for SRR6322411.sra Read 1822289 spots for SRR6322411.sra Written 1822289 spots for SRR6322411.sra Read 1822292 spots for SRR6322411.sra Written 1822292 spots for SRR6322411.sra Read 1822289 spots for SRR6322411.sra Written 1822289 spots for SRR6322411.sra Read 1822289 spots for SRR6322411.sra Written 1822289 spots for SRR6322411.sra Read 1822289 spots for SRR6322411.sra Written 1822289 spots for SRR6322411.sra Read 1822289 spots for SRR6322411.sra Written 1822289 spots for SRR6322411.sra Read 1822289 spots for SRR6322411.sra Written 1822289 spots for SRR6322411.sra Read 1822289 spots for SRR6322411.sra Written 1822289 spots for SRR6322411.sra Read 1822289 spots for SRR6322411.sra Written 1822289 spots for SRR6322411.sra Read 1822289 spots for SRR6322411.sra Written 1822289 spots for SRR6322411.sra Read 1822289 spots for SRR6322411.sra Written 1822289 spots for SRR6322411.sra Read 1822289 spots for SRR6322411.sra Written 1822289 spots for SRR6322411.sra Read 1822289 spots for SRR6322411.sra Written 1822289 spots for SRR6322411.sra Read 1822289 spots for SRR6322411.sra Written 1822289 spots for SRR6322411.sra Read 1822289 spots for SRR6322411.sra Written 1822289 spots for SRR6322411.sra Read 1822289 spots for SRR6322411.sra Written 1822289 spots for SRR6322411.sra Read 1822289 spots for SRR6322411.sra Written 1822289 spots for SRR6322411.sra SRR ids: ['SRR6322411.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_qqsr4u4c SRR6322411.sra spots: 36445783 blocks: [[1, 1822289], [1822290, 3644578], [3644579, 5466867], [5466868, 7289156], [7289157, 9111445], [9111446, 10933734], [10933735, 12756023], [12756024, 14578312], [14578313, 16400601], [16400602, 18222890], [18222891, 20045179], [20045180, 21867468], [21867469, 23689757], [23689758, 25512046], [25512047, 27334335], [27334336, 29156624], [29156625, 30978913], [30978914, 32801202], [32801203, 34623491], [34623492, 36445783]] SRR6322411 file size 6341850 SRR6322411 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR6322411 SRR6322411_1.fastq Input file: SRR6322411_1.fastq trimmed: SRR6322411-trimmed.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- minimum overlap length for adapter detection (-k): inf -- number of concurrent threads (-t): 20 Sat Dec 7 10:27:52 2024 >> started Sat Dec 7 10:28:17 2024 >> done (25.445s) 36445783 reads processed; of these: 11182 ( 0.03%) short reads filtered out after trimming by size control 44403 ( 0.12%) empty reads filtered out after trimming by size control 36390198 (99.85%) reads available; of these: 676513 ( 1.86%) trimmed reads available after processing 35713685 (98.14%) untrimmed reads available after processing Length distribution of reads after trimming: length count percentage 18 769 0.00% 19 711 0.00% 20 820 0.00% 21 940 0.00% 22 1156 0.00% 23 1294 0.00% 24 1535 0.00% 25 2086 0.01% 26 2031 0.01% 27 2155 0.01% 28 2391 0.01% 29 2521 0.01% 30 2748 0.01% 31 3195 0.01% 32 3601 0.01% 33 4250 0.01% 34 4961 0.01% 35 5682 0.02% 36 6769 0.02% 37 7513 0.02% 38 8766 0.02% 39 10679 0.03% 40 13154 0.04% 41 16199 0.04% 42 20475 0.06% 43 25817 0.07% 44 32753 0.09% 45 43566 0.12% 46 59366 0.16% 47 81429 0.22% 48 126968 0.35% 49 180213 0.50% 50 35713685 98.14% 36390198 reads passed initial QC criterion=sequence-density sequence-density=1.93 sequence-density-rank=1 fanout-score=1.99 fanout-score-rank=28 prefix-density=1.94 prefix-fanout=2.0 sequence=TTTGGCTTTGGC criterion=fanout-score sequence-density=0.09 sequence-density-rank=31 fanout-score=14.90 fanout-score-rank=1 prefix-density=0.30 prefix-fanout=4.7 sequence=CGCCGCCGCCGCGGGAGGAGGACGCGATCTCCACCACCGGGTGGCCCGGGGGCACCACGAACGCTGAGCCGACGGACACCCGGGCGCGGATCCTCTCGTACCTTCCCTGCTGCTGTTGCTGCTGCTGT Started job on | Dec 07 10:28:27 Started mapping on | Dec 07 10:28:28 Finished on | Dec 07 10:28:53 Mapping speed, Million of reads per hour | 5240.19 Number of input reads | 36390198 Average input read length | 49 UNIQUE READS: Uniquely mapped reads number | 27801953 Uniquely mapped reads % | 76.40% Average mapped length | 49.82 Number of splices: Total | 2458625 Number of splices: Annotated (sjdb) | 2345469 Number of splices: GT/AG | 2404516 Number of splices: GC/AG | 32770 Number of splices: AT/AC | 1070 Number of splices: Non-canonical | 20269 Mismatch rate per base, % | 0.24% Deletion rate per base | 0.00% Deletion average length | 1.49 Insertion rate per base | 0.00% Insertion average length | 1.45 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 6347240 % of reads mapped to multiple loci | 17.44% Number of reads mapped to too many loci | 2106049 % of reads mapped to too many loci | 5.79% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 0.30% % of reads unmapped: other | 0.07% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 2241005 2241005 2241005 N_multimapping 6347240 6347240 6347240 N_noFeature 1028172 27322710 1330683 N_ambiguous 199827 879 22845 UnstrandedReadsAssigned:26573954 PositiveStrandReadsAssigned:478364 NegativeStrandReadsAssigned:26448425 Dataset is classified negative stranded MeadianReadLen=50 20thPercentileLength=50 echo kmer=45 SRR6322411 Starting Kallisto single end mapping to ensembl reference transcriptome. kmer=31 [quant] fragment length distribution is truncated gaussian with mean = 100, sd = 20 [index] k-mer length: 31 [index] number of targets: 52,972 [index] number of k-mers: 66,720,672 [index] number of equivalence classes: 111,837 [quant] running in single-end mode [quant] will process file 1: SRR6322411-trimmed.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 36,390,198 reads, 31,405,471 reads pseudoaligned [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,219 rounds 52973 SRR6322411.ke.tsv 35125 SRR6322411.se.tsv 88098 total ==> SRR6322411.ke.tsv <== target_id length eff_length est_counts tpm PNS24245 936 837 5.57244e-07 2.72483e-08 PNS24247 1044 945 71.9383 3.11564 PNS24249 1928 1829 224.378 5.02096 PNS24246 1044 945 71.9383 3.11564 PNS24248 1044 945 71.9383 3.11564 PNS24244 1471 1372 33.8069 1.00849 PNS24243 293 194 0 0 KQK14069 1603 1504 670.578 18.2482 KQK14071 474 375 196.645 21.462 ==> SRR6322411.se.tsv <== BRADI_1g14170v3 1132 BRADI_1g53295v3 699 BRADI_1g59795v3 312 BRADI_1g07683v3 0 BRADI_1g00485v3 2 BRADI_1g20270v3 42 BRADI_1g74790v3 0 BRADI_1g09890v3 0 BRADI_1g77505v3 157 BRADI_1g48960v3 8 SRR6322411 completed mapping pipeline successfully