Starting /dee2/code/volunteer_pipeline.sh SRR6322415 current disk space = 1543467618304 free memory = 1595192308 SRR6322415 SRAfilesize ef51599dd63955216c02a02dc9cd7842 SRR6322415.sra SRR6322415.sra file validated SRR6322415 is single end SRR6322415 is conventional basespace SRR6322415 read1 length is 50 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR6322415_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 50 %GC 52 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.35875 33.0 33.0 34.0 31.0 34.0 2 32.4565 34.0 33.0 34.0 30.0 34.0 3 32.58725 34.0 33.0 34.0 31.0 34.0 4 32.44925 34.0 33.0 34.0 31.0 34.0 5 32.3955 34.0 33.0 34.0 31.0 34.0 6 35.8945 38.0 36.0 38.0 31.0 38.0 7 36.27325 38.0 37.0 38.0 33.0 38.0 8 36.51475 38.0 38.0 38.0 34.0 38.0 9 36.51425 38.0 38.0 38.0 34.0 38.0 10 36.701 38.0 38.0 38.0 34.0 38.0 11 36.67125 38.0 38.0 38.0 34.0 38.0 12 36.6125 38.0 38.0 38.0 34.0 38.0 13 36.63925 38.0 38.0 38.0 34.0 38.0 14 36.67675 38.0 38.0 38.0 34.0 38.0 15 36.711 38.0 38.0 38.0 34.0 38.0 16 36.69425 38.0 38.0 38.0 34.0 38.0 17 36.645 38.0 38.0 38.0 34.0 38.0 18 36.61075 38.0 38.0 38.0 34.0 38.0 19 36.788 38.0 38.0 38.0 35.0 38.0 20 36.6945 38.0 38.0 38.0 34.0 38.0 21 36.659 38.0 38.0 38.0 34.0 38.0 22 36.694 38.0 38.0 38.0 34.0 38.0 23 36.767 38.0 38.0 38.0 35.0 38.0 24 36.64825 38.0 38.0 38.0 34.0 38.0 25 36.65175 38.0 38.0 38.0 34.0 38.0 26 36.633 38.0 38.0 38.0 34.0 38.0 27 36.56525 38.0 38.0 38.0 34.0 38.0 28 36.59175 38.0 38.0 38.0 34.0 38.0 29 36.63925 38.0 38.0 38.0 34.0 38.0 30 36.63325 38.0 38.0 38.0 34.0 38.0 31 36.64725 38.0 38.0 38.0 34.0 38.0 32 36.5755 38.0 38.0 38.0 34.0 38.0 33 36.636 38.0 38.0 38.0 34.0 38.0 34 36.53625 38.0 38.0 38.0 34.0 38.0 35 36.5315 38.0 38.0 38.0 34.0 38.0 36 36.56175 38.0 38.0 38.0 34.0 38.0 37 36.5285 38.0 38.0 38.0 34.0 38.0 38 36.52 38.0 38.0 38.0 34.0 38.0 39 36.57675 38.0 38.0 38.0 34.0 38.0 40 36.45275 38.0 38.0 38.0 34.0 38.0 41 36.50425 38.0 38.0 38.0 34.0 38.0 42 36.3855 38.0 38.0 38.0 34.0 38.0 43 36.50925 38.0 38.0 38.0 34.0 38.0 44 36.4275 38.0 38.0 38.0 34.0 38.0 45 36.34825 38.0 38.0 38.0 34.0 38.0 46 36.32975 38.0 38.0 38.0 34.0 38.0 47 36.364 38.0 38.0 38.0 34.0 38.0 48 36.32625 38.0 38.0 38.0 34.0 38.0 49 36.207 38.0 38.0 38.0 34.0 38.0 50 36.21775 38.0 38.0 38.0 34.0 38.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0 1101 2 0.0 1101 3 0.0 1101 4 0.0 1101 5 0.0 1101 6 0.0 1101 7 0.0 1101 8 0.0 1101 9 0.0 1101 10 0.0 1101 11 0.0 1101 12 0.0 1101 13 0.0 1101 14 0.0 1101 15 0.0 1101 16 0.0 1101 17 0.0 1101 18 0.0 1101 19 0.0 1101 20 0.0 1101 21 0.0 1101 22 0.0 1101 23 0.0 1101 24 0.0 1101 25 0.0 1101 26 0.0 1101 27 0.0 1101 28 0.0 1101 29 0.0 1101 30 0.0 1101 31 0.0 1101 32 0.0 1101 33 0.0 1101 34 0.0 1101 35 0.0 1101 36 0.0 1101 37 0.0 1101 38 0.0 1101 39 0.0 1101 40 0.0 1101 41 0.0 1101 42 0.0 1101 43 0.0 1101 44 0.0 1101 45 0.0 1101 46 0.0 1101 47 0.0 1101 48 0.0 1101 49 0.0 1101 50 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5.0 3 0.0 4 1.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 1.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 2.0 21 2.0 22 2.0 23 5.0 24 6.0 25 9.0 26 22.0 27 30.0 28 43.0 29 56.0 30 63.0 31 83.0 32 102.0 33 120.0 34 159.0 35 264.0 36 554.0 37 2471.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 36.72544080604534 13.350125944584383 8.337531486146096 41.58690176322418 2 25.7 20.625 33.425 20.25 3 21.025 24.4 22.425 32.15 4 27.900000000000002 29.549999999999997 18.9 23.65 5 24.5 31.95 21.925 21.625 6 19.650000000000002 30.2 24.2 25.95 7 19.025 15.975 38.0 27.0 8 22.075 18.275 25.4 34.25 9 20.575 18.725 29.525000000000002 31.175000000000004 10 25.374999999999996 31.7 19.775000000000002 23.150000000000002 11 29.275000000000002 19.75 17.299999999999997 33.675 12 28.799999999999997 17.75 22.15 31.3 13 23.9 21.85 24.5 29.75 14 24.825 23.150000000000002 25.424999999999997 26.6 15 24.975 23.325000000000003 23.75 27.950000000000003 16 24.625 23.075000000000003 23.0 29.299999999999997 17 24.875 23.925 23.275000000000002 27.925 18 23.3 24.5 25.6 26.6 19 24.0 23.175 25.75 27.075 20 25.4 23.075000000000003 25.35 26.174999999999997 21 25.124999999999996 22.675 24.825 27.375 22 25.85 23.0 22.75 28.4 23 23.200000000000003 22.875 24.85 29.075 24 24.85 22.825 26.0 26.325 25 24.925 23.125 23.125 28.825 26 23.799999999999997 24.2 25.674999999999997 26.325 27 24.7 22.35 23.65 29.299999999999997 28 25.525 23.125 24.55 26.8 29 25.1 22.875 25.15 26.875 30 22.7 22.45 27.075 27.775 31 24.15 23.425 25.025 27.400000000000002 32 25.3 24.325 23.400000000000002 26.974999999999998 33 23.599999999999998 23.45 24.4 28.549999999999997 34 23.674999999999997 25.074999999999996 22.725 28.525 35 23.825 25.575 24.0 26.6 36 24.675 24.15 24.375 26.8 37 25.474999999999998 23.425 23.549999999999997 27.55 38 23.05 24.275 25.0 27.675 39 25.0 21.75 25.75 27.500000000000004 40 24.275 22.3 24.45 28.975 41 24.575 23.549999999999997 25.474999999999998 26.400000000000002 42 23.575 23.1 25.775 27.55 43 25.424999999999997 20.775 25.324999999999996 28.475 44 22.15 24.65 25.775 27.425 45 25.224999999999998 22.075 25.05 27.650000000000002 46 25.474999999999998 22.125 25.1 27.3 47 23.125 23.525 26.325 27.025 48 24.125 22.675 23.724999999999998 29.475 49 24.8 22.6 24.275 28.325 50 24.6 23.974999999999998 23.825 27.6 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.5 18 1.0 19 1.0 20 1.0 21 2.5 22 4.0 23 3.5 24 3.0 25 5.0 26 7.0 27 9.5 28 12.0 29 21.0 30 30.0 31 31.5 32 33.0 33 43.5 34 54.0 35 83.0 36 112.0 37 125.0 38 138.0 39 154.0 40 170.0 41 198.0 42 226.0 43 240.5 44 255.0 45 238.5 46 222.0 47 242.0 48 262.0 49 287.5 50 313.0 51 325.5 52 338.0 53 320.5 54 303.0 55 273.5 56 244.0 57 244.0 58 244.0 59 217.5 60 191.0 61 176.0 62 161.0 63 147.0 64 133.0 65 117.5 66 102.0 67 102.0 68 102.0 69 102.0 70 102.0 71 88.0 72 74.0 73 67.5 74 61.0 75 50.0 76 39.0 77 35.0 78 31.0 79 27.0 80 23.0 81 14.0 82 5.0 83 3.5 84 2.0 85 2.0 86 2.0 87 1.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.75 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 50 4000.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 88.55 #Duplication Level Percentage of deduplicated Percentage of total 1 93.39356295878035 82.69999999999999 2 4.206662902315076 7.449999999999999 3 1.0446075663466967 2.775 4 0.4799548277809147 1.7000000000000002 5 0.4799548277809147 2.125 6 0.08469791078486731 0.44999999999999996 7 0.08469791078486731 0.525 8 0.0282326369282891 0.2 9 0.0564652738565782 0.44999999999999996 >10 0.1411631846414455 1.625 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GGCAATTTCAAGCACTCTTTGACTCTCTTTTCAAAGTCCTTTTCATCTTT 20 0.5 No Hit CTCGATCACGCGGACGTGCTGGCCGGAGCCGCCCACGTCGAACTGGTCGT 12 0.3 No Hit GTCAACGCGAGCTGATGACTCGCGCTTACTAGGCATTCCTCGTTGAAGAC 12 0.3 No Hit CAGGGACGTAGTCAACGCGAGCTGATGACTCGCGCTTACTAGGCATTCCT 11 0.27499999999999997 No Hit GCAATTTCAAGCACTCTTTGACTCTCTTTTCAAAGTCCTTTTCATCTTTC 10 0.25 No Hit CAAAGGGCAGGGACGTAGTCAACGCGAGCTGATGACTCGCGCTTACTAGG 9 0.22499999999999998 No Hit GTAGTCAACGCGAGCTGATGACTCGCGCTTACTAGGCATTCCTCGTTGAA 9 0.22499999999999998 No Hit CGTAGTCAACGCGAGCTGATGACTCGCGCTTACTAGGCATTCCTCGTTGA 8 0.2 No Hit CAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCACAAGCCAGTTATCCC 7 0.17500000000000002 No Hit CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCA 7 0.17500000000000002 No Hit CAAGATTACCCGGGCCTGTCGGCCAAGGCTATATACTCGTTGAATACATC 7 0.17500000000000002 No Hit CTTTGACTCTCTTTTCAAAGTCCTTTTCATCTTTCCCTCGCGGTACTTGT 6 0.15 No Hit CTCGCCTGTATTTAGCCTTGGACGGAGTCTACCGCCCGATTTGGGCTGCA 6 0.15 No Hit CGAGCCTCCACCAGAGTTTCCTCTGGCTTCGCCCCGCTCAGGCATAGTTC 6 0.15 No Hit GACGTAGTCAACGCGAGCTGATGACTCGCGCTTACTAGGCATTCCTCGTT 5 0.125 No Hit CGACTGTTCAGCGCTCGGGGAAAGCCCCCGAAGGGGCGACTCCCGGTCCG 5 0.125 No Hit CTCGTAATGGGCTCCAGCTATCCTGAGGGAAACTTCGGAGGGAACCAGCT 5 0.125 No Hit GGATCAGCGAGCAAAGCCCGCGTCAGCCTTTTATCTAATAAATGCGCCCC 5 0.125 No Hit CTCCGATCCCGAAGGCCAACACAATAGGACCGGAATCCTATGATGTTATC 5 0.125 No Hit GTAGCGCGCGTGCGGCCCAGAACATCTAAGGGCATCACAGACCTGTTATT 5 0.125 No Hit CAGCTATCCTGAGGGAAACTTCGGAGGGAACCAGCTACTAGATGGTTCGA 5 0.125 No Hit ACGATTTGCACGTCAGTATCGCTTCGAGCCTCCACCAGAGTTTCCTCTGG 5 0.125 No Hit CGCATCGCCAGTTCTGCTTACCAAAAATGGCCCACTTGGAGCTCCCGATT 5 0.125 No Hit GTGCATCCCACACTCTCTCCAACTCAAACCCACAAATAACACACCGTAAT 5 0.125 No Hit CCAAGATTACCCGGGCCTGTCGGCCAAGGCTATATACTCGTTGAATACAT 5 0.125 No Hit GGCGGCGGGCGCAGGCGCCGCTTGCTAGCTTGGATTCTGACTTAGAGGCG 5 0.125 No Hit GAAACTTCGGAGGGAACCAGCTACTAGATGGTTCGATTAGTCTTTCGCCC 5 0.125 No Hit CACGCTTTCACGGTTCGTATTCGTACTGGAAATCAGAATCAAACGAGCTT 5 0.125 No Hit CGACAGGCGTGCTCCAACTCGAACCCTTCACAGAAGATCAGGGTCGGCCA 5 0.125 No Hit CCTCGATCACGCGGACGTGCTGGCCGGAGCCGCCCACGTCGAACTGGTCG 5 0.125 No Hit GTACTTGTTCGCTATCGGTCTCTCGCCTGTATTTAGCCTTGGACGGAGTC 5 0.125 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.025 0.0 0.0 0.0 0.0 4 0.025 0.0 0.0 0.0 0.0 5 0.025 0.0 0.0 0.0 0.0 6 0.025 0.0 0.0 0.0 0.0 7 0.025 0.0 0.0 0.0 0.0 8 0.025 0.0 0.0 0.0 0.0 9 0.05 0.0 0.0 0.0 0.0 10 0.05 0.0 0.0 0.0 0.0 11 0.05 0.0 0.0 0.0 0.0 12 0.05 0.0 0.0 0.0 0.0 13 0.05 0.0 0.0 0.0 0.0 14 0.05 0.0 0.0 0.0 0.0 15 0.05 0.0 0.0 0.0 0.0 16 0.05 0.0 0.0 0.0 0.0 17 0.05 0.0 0.0 0.0 0.0 18 0.05 0.0 0.0 0.0 0.0 19 0.05 0.0 0.0 0.0 0.0 20 0.05 0.0 0.0 0.0 0.0 21 0.05 0.0 0.0 0.0 0.0 22 0.05 0.0 0.0 0.0 0.0 23 0.05 0.0 0.0 0.0 0.0 24 0.05 0.0 0.0 0.0 0.0 25 0.05 0.0 0.0 0.0 0.0 26 0.05 0.0 0.0 0.0 0.0 27 0.05 0.0 0.0 0.0 0.0 28 0.05 0.0 0.0 0.0 0.0 29 0.05 0.0 0.0 0.0 0.0 30 0.05 0.0 0.0 0.0 0.0 31 0.05 0.0 0.0 0.0 0.0 32 0.05 0.0 0.0 0.0 0.0 33 0.05 0.0 0.0 0.0 0.0 34 0.05 0.0 0.0 0.0 0.0 35 0.05 0.0 0.0 0.0 0.0 36 0.075 0.0 0.0 0.0 0.0 37 0.1 0.0 0.0 0.0 0.0 38 0.1 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content pass >>END_MODULE Read 1130598 spots for SRR6322415.sra Written 1130598 spots for SRR6322415.sra Read 1130598 spots for SRR6322415.sra Written 1130598 spots for SRR6322415.sra Read 1130598 spots for SRR6322415.sra Written 1130598 spots for SRR6322415.sra Read 1130598 spots for SRR6322415.sra Written 1130598 spots for SRR6322415.sra Read 1130598 spots for SRR6322415.sra Written 1130598 spots for SRR6322415.sra Read 1130598 spots for SRR6322415.sra Written 1130598 spots for SRR6322415.sra Read 1130598 spots for SRR6322415.sra Written 1130598 spots for SRR6322415.sra Read 1130598 spots for SRR6322415.sra Written 1130598 spots for SRR6322415.sra Read 1130598 spots for SRR6322415.sra Written 1130598 spots for SRR6322415.sra Read 1130598 spots for SRR6322415.sra Written 1130598 spots for SRR6322415.sra Read 1130598 spots for SRR6322415.sra Written 1130598 spots for SRR6322415.sra Read 1130608 spots for SRR6322415.sra Written 1130608 spots for SRR6322415.sra Read 1130598 spots for SRR6322415.sra Written 1130598 spots for SRR6322415.sra Read 1130598 spots for SRR6322415.sra Written 1130598 spots for SRR6322415.sra Read 1130598 spots for SRR6322415.sra Written 1130598 spots for SRR6322415.sra Read 1130598 spots for SRR6322415.sra Written 1130598 spots for SRR6322415.sra Read 1130598 spots for SRR6322415.sra Written 1130598 spots for SRR6322415.sra Read 1130598 spots for SRR6322415.sra Written 1130598 spots for SRR6322415.sra Read 1130598 spots for SRR6322415.sra Written 1130598 spots for SRR6322415.sra Read 1130598 spots for SRR6322415.sra Written 1130598 spots for SRR6322415.sra SRR ids: ['SRR6322415.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_z771wxsc SRR6322415.sra spots: 22611970 blocks: [[1, 1130598], [1130599, 2261196], [2261197, 3391794], [3391795, 4522392], [4522393, 5652990], [5652991, 6783588], [6783589, 7914186], [7914187, 9044784], [9044785, 10175382], [10175383, 11305980], [11305981, 12436578], [12436579, 13567176], [13567177, 14697774], [14697775, 15828372], [15828373, 16958970], [16958971, 18089568], [18089569, 19220166], [19220167, 20350764], [20350765, 21481362], [21481363, 22611970]] SRR6322415 file size 3930556 SRR6322415 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR6322415 SRR6322415_1.fastq Input file: SRR6322415_1.fastq trimmed: SRR6322415-trimmed.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- minimum overlap length for adapter detection (-k): inf -- number of concurrent threads (-t): 20 Sat Dec 7 10:33:13 2024 >> started Sat Dec 7 10:33:30 2024 >> done (17.822s) 22611970 reads processed; of these: 7765 ( 0.03%) short reads filtered out after trimming by size control 31259 ( 0.14%) empty reads filtered out after trimming by size control 22572946 (99.83%) reads available; of these: 362875 ( 1.61%) trimmed reads available after processing 22210071 (98.39%) untrimmed reads available after processing Length distribution of reads after trimming: length count percentage 18 389 0.00% 19 342 0.00% 20 374 0.00% 21 486 0.00% 22 580 0.00% 23 646 0.00% 24 814 0.00% 25 1036 0.00% 26 1084 0.00% 27 1057 0.00% 28 1196 0.01% 29 1274 0.01% 30 1427 0.01% 31 1666 0.01% 32 1882 0.01% 33 2197 0.01% 34 2454 0.01% 35 2753 0.01% 36 3323 0.01% 37 3954 0.02% 38 4777 0.02% 39 5560 0.02% 40 6881 0.03% 41 8316 0.04% 42 10728 0.05% 43 13007 0.06% 44 16752 0.07% 45 22936 0.10% 46 32203 0.14% 47 44136 0.20% 48 68723 0.30% 49 99922 0.44% 50 22210071 98.39% 22572946 reads passed initial QC criterion=sequence-density sequence-density=0.15 sequence-density-rank=1 fanout-score=2.28 fanout-score-rank=29 prefix-density=0.18 prefix-fanout=2.0 sequence=AGCTGGGCTGCTCCCGGTTCGCTCGCCGTTACTAGGGGAATCCTTGTAAGTTTCTTCTCCTCC criterion=fanout-score sequence-density=0.01 sequence-density-rank=41 fanout-score=37.32 fanout-score-rank=1 prefix-density=0.21 prefix-fanout=1.0 sequence=CGGCTGCTGGAGGGTAGGAGCGTCTGCTGGCATCGGAGAAGGTGAAGAAGAGAAGGTTCAGGGCCAGAAGCGCAACGATCAGTTCCACTTTCTTCGCCATGGCTG Started job on | Dec 07 10:33:45 Started mapping on | Dec 07 10:33:46 Finished on | Dec 07 10:34:17 Mapping speed, Million of reads per hour | 2621.37 Number of input reads | 22572946 Average input read length | 49 UNIQUE READS: Uniquely mapped reads number | 15107427 Uniquely mapped reads % | 66.93% Average mapped length | 49.84 Number of splices: Total | 2348861 Number of splices: Annotated (sjdb) | 2266420 Number of splices: GT/AG | 2318489 Number of splices: GC/AG | 25567 Number of splices: AT/AC | 1068 Number of splices: Non-canonical | 3737 Mismatch rate per base, % | 0.16% Deletion rate per base | 0.00% Deletion average length | 1.63 Insertion rate per base | 0.00% Insertion average length | 1.40 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 1679265 % of reads mapped to multiple loci | 7.44% Number of reads mapped to too many loci | 5623087 % of reads mapped to too many loci | 24.91% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 0.43% % of reads unmapped: other | 0.30% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 5786254 5786254 5786254 N_multimapping 1679265 1679265 1679265 N_noFeature 472882 14678671 587845 N_ambiguous 351368 730 38163 UnstrandedReadsAssigned:14283177 PositiveStrandReadsAssigned:428026 NegativeStrandReadsAssigned:14481419 Dataset is classified negative stranded MeadianReadLen=50 20thPercentileLength=50 echo kmer=45 SRR6322415 Starting Kallisto single end mapping to ensembl reference transcriptome. kmer=31 [quant] fragment length distribution is truncated gaussian with mean = 100, sd = 20 [index] k-mer length: 31 [index] number of targets: 52,972 [index] number of k-mers: 66,720,672 [index] number of equivalence classes: 111,837 [quant] running in single-end mode [quant] will process file 1: SRR6322415-trimmed.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 22,572,946 reads, 15,398,855 reads pseudoaligned [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,086 rounds 52973 SRR6322415.ke.tsv 35125 SRR6322415.se.tsv 88098 total ==> SRR6322415.ke.tsv <== target_id length eff_length est_counts tpm PNS24245 936 837 90.3537 10.4693 PNS24247 1044 945 11.6753 1.19821 PNS24249 1928 1829 28.0153 1.48552 PNS24246 1044 945 11.6753 1.19821 PNS24248 1044 945 11.6753 1.19821 PNS24244 1471 1372 63.6051 4.4961 PNS24243 293 194 0 0 KQK14069 1603 1504 2121.25 136.786 KQK14071 474 375 786.069 203.295 ==> SRR6322415.se.tsv <== BRADI_1g14170v3 3064 BRADI_1g53295v3 155 BRADI_1g59795v3 151 BRADI_1g07683v3 0 BRADI_1g00485v3 2 BRADI_1g20270v3 693 BRADI_1g74790v3 69 BRADI_1g09890v3 16 BRADI_1g77505v3 151 BRADI_1g48960v3 0 SRR6322415 completed mapping pipeline successfully