Starting /dee2/code/volunteer_pipeline.sh SRR6322431 current disk space = 1543139356672 free memory = 1592642824 SRR6322431 SRAfilesize 6f56a2ca74f18197e632e87c3fc5f164 SRR6322431.sra SRR6322431.sra file validated SRR6322431 is single end SRR6322431 is conventional basespace SRR6322431 read1 length is 50 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR6322431_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 50 %GC 50 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.455 32.0 32.0 32.0 32.0 32.0 2 30.16625 32.0 32.0 32.0 32.0 32.0 3 35.2475 37.0 32.0 37.0 32.0 37.0 4 34.365 37.0 32.0 37.0 27.0 37.0 5 35.665 37.0 37.0 37.0 32.0 37.0 6 39.979 41.0 41.0 41.0 37.0 41.0 7 40.161 41.0 41.0 41.0 37.0 41.0 8 40.3 41.0 41.0 41.0 41.0 41.0 9 39.908 41.0 41.0 41.0 37.0 41.0 10 40.21775 41.0 41.0 41.0 37.0 41.0 11 38.64175 41.0 41.0 41.0 32.0 41.0 12 39.87325 41.0 41.0 41.0 37.0 41.0 13 40.241 41.0 41.0 41.0 37.0 41.0 14 40.28025 41.0 41.0 41.0 41.0 41.0 15 39.463 41.0 41.0 41.0 37.0 41.0 16 39.37875 41.0 41.0 41.0 37.0 41.0 17 40.22525 41.0 41.0 41.0 37.0 41.0 18 40.33725 41.0 41.0 41.0 41.0 41.0 19 40.19675 41.0 41.0 41.0 41.0 41.0 20 39.754 41.0 41.0 41.0 37.0 41.0 21 37.867 41.0 37.0 41.0 27.0 41.0 22 39.673 41.0 41.0 41.0 37.0 41.0 23 37.75325 41.0 37.0 41.0 27.0 41.0 24 39.45 41.0 41.0 41.0 37.0 41.0 25 39.4925 41.0 41.0 41.0 37.0 41.0 26 37.928 41.0 37.0 41.0 27.0 41.0 27 33.108 41.0 27.0 41.0 12.0 41.0 28 34.738 41.0 32.0 41.0 22.0 41.0 29 38.61725 41.0 37.0 41.0 32.0 41.0 30 39.0555 41.0 41.0 41.0 37.0 41.0 31 38.88225 41.0 41.0 41.0 37.0 41.0 32 38.26675 41.0 37.0 41.0 32.0 41.0 33 32.0655 37.0 22.0 41.0 12.0 41.0 34 37.23425 41.0 37.0 41.0 27.0 41.0 35 38.80925 41.0 41.0 41.0 32.0 41.0 36 39.713 41.0 41.0 41.0 37.0 41.0 37 39.66275 41.0 41.0 41.0 37.0 41.0 38 38.388 41.0 41.0 41.0 32.0 41.0 39 38.25875 41.0 41.0 41.0 32.0 41.0 40 38.65325 41.0 41.0 41.0 32.0 41.0 41 39.232 41.0 41.0 41.0 37.0 41.0 42 39.419 41.0 41.0 41.0 37.0 41.0 43 39.6035 41.0 41.0 41.0 37.0 41.0 44 38.5905 41.0 41.0 41.0 32.0 41.0 45 38.7265 41.0 41.0 41.0 32.0 41.0 46 31.88175 37.0 22.0 41.0 12.0 41.0 47 36.56275 41.0 37.0 41.0 27.0 41.0 48 37.26525 41.0 37.0 41.0 27.0 41.0 49 39.229 41.0 41.0 41.0 37.0 41.0 50 39.4835 41.0 41.0 41.0 37.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 18 1.0 19 1.0 20 0.0 21 1.0 22 3.0 23 3.0 24 4.0 25 3.0 26 6.0 27 9.0 28 24.0 29 34.0 30 42.0 31 65.0 32 79.0 33 130.0 34 121.0 35 174.0 36 261.0 37 350.0 38 584.0 39 1047.0 40 1058.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 37.30932733183296 9.427356839209802 6.976744186046512 46.28657164291073 2 22.68929503916449 9.321148825065274 43.42036553524804 24.56919060052219 3 21.3 13.425 26.55 38.725 4 25.7 19.525000000000002 24.6 30.175 5 29.849999999999998 22.825 25.4 21.925 6 25.45 27.575 25.674999999999997 21.3 7 19.225 22.75 40.5 17.525 8 19.375 22.05 31.8 26.775 9 19.675 19.775000000000002 37.05 23.5 10 23.549999999999997 28.599999999999998 28.375 19.475 11 27.125 22.3 25.6 24.975 12 24.3 22.5 26.950000000000003 26.25 13 24.975 23.125 27.250000000000004 24.65 14 24.925 22.725 26.3 26.05 15 25.35 22.7 27.175 24.775 16 24.425 22.5 26.525 26.55 17 25.424999999999997 22.175 26.825 25.575 18 25.074999999999996 23.825 25.8 25.3 19 25.074999999999996 22.825 23.974999999999998 28.125 20 25.15 23.200000000000003 26.375 25.275 21 24.7 21.475 26.924999999999997 26.900000000000002 22 25.05 23.525 24.525 26.900000000000002 23 24.3 23.200000000000003 26.1 26.400000000000002 24 22.425 23.150000000000002 28.175 26.25 25 25.7 23.05 26.325 24.925 26 25.775 23.425 25.825 24.975 27 24.2 24.075 26.900000000000002 24.825 28 27.075 23.200000000000003 26.125 23.599999999999998 29 23.474999999999998 23.3 27.675 25.55 30 22.85 23.599999999999998 27.325 26.224999999999998 31 27.05 22.425 24.025 26.5 32 24.875 22.975 27.175 24.975 33 24.125 21.5 28.249999999999996 26.125 34 25.25 23.549999999999997 23.75 27.450000000000003 35 22.875 23.225 28.000000000000004 25.900000000000002 36 24.275 22.775000000000002 26.6 26.35 37 25.4 22.0 28.475 24.125 38 24.474999999999998 22.225 27.125 26.174999999999997 39 24.8 23.05 27.125 25.025 40 22.95 24.975 26.375 25.7 41 23.925 22.5 25.874999999999996 27.700000000000003 42 24.4 24.125 26.200000000000003 25.275 43 23.75 22.2 26.150000000000002 27.900000000000002 44 25.525 23.549999999999997 26.700000000000003 24.224999999999998 45 24.05 22.075 28.599999999999998 25.275 46 26.8 22.075 26.200000000000003 24.925 47 25.7 23.400000000000002 26.25 24.65 48 24.65 21.55 27.325 26.474999999999998 49 26.0 21.825 26.275 25.900000000000002 50 26.075 23.200000000000003 24.95 25.775 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.5 4 1.0 5 0.5 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 3.5 24 7.0 25 7.5 26 8.0 27 10.5 28 13.0 29 14.5 30 16.0 31 30.5 32 45.0 33 60.5 34 76.0 35 97.5 36 119.0 37 142.0 38 165.0 39 189.0 40 213.0 41 256.0 42 299.0 43 320.0 44 341.0 45 318.5 46 296.0 47 320.5 48 345.0 49 320.5 50 296.0 51 278.0 52 260.0 53 254.0 54 248.0 55 241.0 56 234.0 57 193.0 58 152.0 59 154.0 60 156.0 61 157.5 62 159.0 63 131.0 64 103.0 65 103.5 66 104.0 67 107.0 68 110.0 69 91.5 70 73.0 71 68.5 72 64.0 73 50.0 74 36.0 75 33.0 76 30.0 77 22.5 78 15.0 79 13.0 80 11.0 81 7.0 82 3.0 83 2.0 84 1.0 85 1.0 86 1.0 87 0.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.025 2 4.25 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 50 4000.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 87.0 #Duplication Level Percentage of deduplicated Percentage of total 1 90.08620689655173 78.375 2 7.672413793103448 13.350000000000001 3 1.1206896551724137 2.9250000000000003 4 0.5172413793103449 1.7999999999999998 5 0.25862068965517243 1.125 6 0.1724137931034483 0.8999999999999999 7 0.028735632183908046 0.17500000000000002 8 0.028735632183908046 0.2 9 0.028735632183908046 0.22499999999999998 >10 0.08620689655172414 0.9249999999999999 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATGATTAACAGCTGCCCTCGACGAAGAAGTCCATTGAACACATTGCGG 17 0.42500000000000004 No Hit CTCCGATCGGGTAACTCTGGCAGCTGCTTTGGGGGAAATTCGGAGTCAAC 10 0.25 No Hit GTTCGATTGGTAGGAAGGGTTGTTTCTGCTGTTGTTGGGTGACGACGGGC 10 0.25 No Hit GGTATGATTAACAGCTGCCCTCGACGAAGAAGTCCATTGAACACATTGCG 9 0.22499999999999998 No Hit CTGGTATCCTTCACTCTCCGCCTTCTTTAAAACGACATAGTTTTGTGGTA 8 0.2 No Hit CTTGTGTAAACTGGTGGACTCTCTGGTGCACATCTCCCAACTTTTGGCTT 7 0.17500000000000002 No Hit GTTATTTTTAAGGTTTTGAGCTTCTTGCCTAGAGATGCGGTACGCATTGG 6 0.15 No Hit CTCATCAAAATACTCCGTCAGACCAGCTTGTGACCTCACTTGCGTAAGTG 6 0.15 No Hit CGGGAAATTCTGACCGTTGAGGCGTGCGATACTGCCAGCACGTGGGTTGT 6 0.15 No Hit CCTGTGCCTTAGGGGTCGAAGATGAATCCACGTCTCCGATCGGGTAACTC 6 0.15 No Hit CTCCAAACCGTTGCGACTCGATTGTCCTTCCTGAGGTTCGATTGGTAGGA 6 0.15 No Hit CCTAGGTTCAAGCTGGTTAGCATTATTGTTTACGTCAAAAACATAGACTG 6 0.15 No Hit CATGGAGTTTGCGTTGGTCTTGAAAGCGATATACTGGTATCCTTCACTCT 5 0.125 No Hit CTCATTTTGCTCATCAAAATACTCCGTCAGACCAGCTTGTGACCTCACTT 5 0.125 No Hit GTTTGTTCATTGATACCAAAGGCCTGGCTAAGCAACTGGGCATTTAAACC 5 0.125 No Hit CTTTTGCCTAGGTTCAAGCTGGTTAGCATTATTGTTTACGTCAAAAACAT 5 0.125 No Hit ATGATTAACAGCTGCCCTCGACGAAGAAGTCCATTGAACACATTGCGGCC 5 0.125 No Hit GTCCTTCCTGAGGTTCGATTGGTAGGAAGGGTTGTTTCTGCTGTTGTTGG 5 0.125 No Hit CGTCATTCTGATTCTGAATTATTCGTGATGTTTGTTCATTGATACCAAAG 5 0.125 No Hit CTGGGATCTGGGCCAGCTGCTGGCAAAACTGCTGCCTCATCACCTGGCAG 5 0.125 No Hit CTTTATTTTTGTCACTGCTGCTACATCGACTAAAACAGCGGTGATCCCTA 5 0.125 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 14 0.0 0.0 0.0 0.0 0.0 15 0.0 0.0 0.0 0.0 0.0 16 0.0 0.0 0.0 0.0 0.0 17 0.0 0.0 0.0 0.0 0.0 18 0.0 0.0 0.0 0.0 0.0 19 0.0 0.0 0.0 0.0 0.0 20 0.0 0.0 0.0 0.0 0.0 21 0.0 0.0 0.0 0.0 0.0 22 0.0 0.0 0.0 0.0 0.0 23 0.0 0.0 0.0 0.0 0.0 24 0.0 0.0 0.0 0.0 0.0 25 0.0 0.0 0.0 0.0 0.0 26 0.0 0.0 0.0 0.0 0.0 27 0.0 0.0 0.0 0.0 0.0 28 0.0 0.0 0.0 0.0 0.0 29 0.0 0.0 0.0 0.0 0.0 30 0.0 0.0 0.0 0.0 0.0 31 0.0 0.0 0.0 0.0 0.0 32 0.0 0.0 0.0 0.0 0.0 33 0.0 0.0 0.0 0.0 0.0 34 0.0 0.0 0.0 0.0 0.0 35 0.0 0.0 0.0 0.0 0.0 36 0.0 0.0 0.0 0.0 0.0 37 0.0 0.0 0.0 0.0 0.0 38 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content pass >>END_MODULE Rejected 1528181 READS because READLEN < 1 Read 1528181 spots for SRR6322431.sra Written 1528181 spots for SRR6322431.sra Rejected 1528181 READS because READLEN < 1 Read 1528181 spots for SRR6322431.sra Written 1528181 spots for SRR6322431.sra Rejected 1528181 READS because READLEN < 1 Read 1528181 spots for SRR6322431.sra Written 1528181 spots for SRR6322431.sra Rejected 1528181 READS because READLEN < 1 Read 1528181 spots for SRR6322431.sra Written 1528181 spots for SRR6322431.sra Rejected 1528181 READS because READLEN < 1 Read 1528181 spots for SRR6322431.sra Written 1528181 spots for SRR6322431.sra Rejected 1528181 READS because READLEN < 1 Read 1528181 spots for SRR6322431.sra Written 1528181 spots for SRR6322431.sra Rejected 1528181 READS because READLEN < 1 Read 1528181 spots for SRR6322431.sra Written 1528181 spots for SRR6322431.sra Rejected 1528181 READS because READLEN < 1 Read 1528181 spots for SRR6322431.sra Written 1528181 spots for SRR6322431.sra Rejected 1528181 READS because READLEN < 1 Read 1528181 spots for SRR6322431.sra Written 1528181 spots for SRR6322431.sra Rejected 1528181 READS because READLEN < 1 Read 1528181 spots for SRR6322431.sra Written 1528181 spots for SRR6322431.sra Rejected 1528181 READS because READLEN < 1 Read 1528181 spots for SRR6322431.sra Written 1528181 spots for SRR6322431.sra Rejected 1528181 READS because READLEN < 1 Read 1528181 spots for SRR6322431.sra Written 1528181 spots for SRR6322431.sra Rejected 1528181 READS because READLEN < 1 Read 1528181 spots for SRR6322431.sra Written 1528181 spots for SRR6322431.sra Rejected 1528181 READS because READLEN < 1 Read 1528181 spots for SRR6322431.sra Written 1528181 spots for SRR6322431.sra Rejected 1528181 READS because READLEN < 1 Read 1528181 spots for SRR6322431.sra Written 1528181 spots for SRR6322431.sra Rejected 1528181 READS because READLEN < 1 Read 1528181 spots for SRR6322431.sra Written 1528181 spots for SRR6322431.sra Rejected 1528197 READS because READLEN < 1 Read 1528197 spots for SRR6322431.sra Written 1528197 spots for SRR6322431.sra Rejected 1528181 READS because READLEN < 1 Read 1528181 spots for SRR6322431.sra Written 1528181 spots for SRR6322431.sra Rejected 1528181 READS because READLEN < 1 Read 1528181 spots for SRR6322431.sra Written 1528181 spots for SRR6322431.sra Rejected 1528181 READS because READLEN < 1 Read 1528181 spots for SRR6322431.sra Written 1528181 spots for SRR6322431.sra SRR ids: ['SRR6322431.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_gzn4dbzt SRR6322431.sra spots: 30563636 blocks: [[1, 1528181], [1528182, 3056362], [3056363, 4584543], [4584544, 6112724], [6112725, 7640905], [7640906, 9169086], [9169087, 10697267], [10697268, 12225448], [12225449, 13753629], [13753630, 15281810], [15281811, 16809991], [16809992, 18338172], [18338173, 19866353], [19866354, 21394534], [21394535, 22922715], [22922716, 24450896], [24450897, 25979077], [25979078, 27507258], [27507259, 29035439], [29035440, 30563636]] SRR6322431 file size 4276310 SRR6322431 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR6322431 SRR6322431_1.fastq Input file: SRR6322431_1.fastq trimmed: SRR6322431-trimmed.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- minimum overlap length for adapter detection (-k): inf -- number of concurrent threads (-t): 20 Sat Dec 7 12:19:00 2024 >> started Sat Dec 7 12:19:19 2024 >> done (18.476s) 30563636 reads processed; of these: 125 ( 0.00%) short reads filtered out after trimming by size control 18192 ( 0.06%) empty reads filtered out after trimming by size control 30545319 (99.94%) reads available; of these: 31 ( 0.00%) trimmed reads available after processing 30545288 (100.00%) untrimmed reads available after processing Length distribution of reads after trimming: length count percentage 18 24 0.00% 19 0 0.00% 20 0 0.00% 21 0 0.00% 22 0 0.00% 23 0 0.00% 24 0 0.00% 25 0 0.00% 26 0 0.00% 27 0 0.00% 28 0 0.00% 29 0 0.00% 30 0 0.00% 31 0 0.00% 32 0 0.00% 33 0 0.00% 34 0 0.00% 35 0 0.00% 36 0 0.00% 37 0 0.00% 38 0 0.00% 39 0 0.00% 40 0 0.00% 41 0 0.00% 42 0 0.00% 43 0 0.00% 44 0 0.00% 45 0 0.00% 46 0 0.00% 47 0 0.00% 48 0 0.00% 49 7 0.00% 50 30545288 100.00% 30545319 reads passed initial QC criterion=sequence-density sequence-density=1.41 sequence-density-rank=1 fanout-score=2.05 fanout-score-rank=25 prefix-density=1.48 prefix-fanout=2.0 sequence=TTTGGCTTTGGC criterion=fanout-score sequence-density=0.01 sequence-density-rank=34 fanout-score=53.13 fanout-score-rank=1 prefix-density=0.20 prefix-fanout=3.7 sequence=TTTATTTATATTTTATTGAGAACACAAACATCAAGTGCTGCACACTTGCGATTTATTTATTTTACTCTACAGTTCTCATATATATTAGTTCATTCGCTCTGATGCCTTGCGACTTGAAGCCGATTCCTCAAAACTCTGGTAGCTCAATGGAGGGAATTTAGTAGTGAAGGCGCCAAACTCTTCTCCCC Started job on | Dec 07 12:19:27 Started mapping on | Dec 07 12:19:27 Finished on | Dec 07 12:19:53 Mapping speed, Million of reads per hour | 4229.35 Number of input reads | 30545319 Average input read length | 49 UNIQUE READS: Uniquely mapped reads number | 22992757 Uniquely mapped reads % | 75.27% Average mapped length | 49.80 Number of splices: Total | 2710785 Number of splices: Annotated (sjdb) | 2588438 Number of splices: GT/AG | 2638111 Number of splices: GC/AG | 30844 Number of splices: AT/AC | 1421 Number of splices: Non-canonical | 40409 Mismatch rate per base, % | 0.36% Deletion rate per base | 0.00% Deletion average length | 1.58 Insertion rate per base | 0.00% Insertion average length | 1.43 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 7084446 % of reads mapped to multiple loci | 23.19% Number of reads mapped to too many loci | 330510 % of reads mapped to too many loci | 1.08% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 0.42% % of reads unmapped: other | 0.03% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 468116 468116 468116 N_multimapping 7084446 7084446 7084446 N_noFeature 1080886 22629770 1191184 N_ambiguous 296701 811 44481 UnstrandedReadsAssigned:21615170 PositiveStrandReadsAssigned:362176 NegativeStrandReadsAssigned:21757092 Dataset is classified negative stranded MeadianReadLen=50 20thPercentileLength=50 echo kmer=45 SRR6322431 Starting Kallisto single end mapping to ensembl reference transcriptome. kmer=31 [quant] fragment length distribution is truncated gaussian with mean = 100, sd = 20 [index] k-mer length: 31 [index] number of targets: 52,972 [index] number of k-mers: 66,720,672 [index] number of equivalence classes: 111,837 [quant] running in single-end mode [quant] will process file 1: SRR6322431-trimmed.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 30,545,319 reads, 27,065,710 reads pseudoaligned [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,150 rounds 52973 SRR6322431.ke.tsv 35125 SRR6322431.se.tsv 88098 total ==> SRR6322431.ke.tsv <== target_id length eff_length est_counts tpm PNS24245 936 837 0 0 PNS24247 1044 945 77.9636 4.5102 PNS24249 1928 1829 240.715 7.19491 PNS24246 1044 945 77.9636 4.5102 PNS24248 1044 945 77.9636 4.5102 PNS24244 1471 1372 91.3946 3.64169 PNS24243 293 194 0 0 KQK14069 1603 1504 1419.38 51.5924 KQK14071 474 375 223.192 32.5375 ==> SRR6322431.se.tsv <== BRADI_1g14170v3 1952 BRADI_1g53295v3 676 BRADI_1g59795v3 251 BRADI_1g07683v3 0 BRADI_1g00485v3 8 BRADI_1g20270v3 325 BRADI_1g74790v3 1 BRADI_1g09890v3 0 BRADI_1g77505v3 253 BRADI_1g48960v3 6 SRR6322431 completed mapping pipeline successfully