Starting /dee2/code/volunteer_pipeline.sh SRR6322441 current disk space = 1543367442432 free memory = 1598535100 SRR6322441 SRAfilesize 66ca80528a640c79f33d0116daefbe63 SRR6322441.sra SRR6322441.sra file validated SRR6322441 is single end SRR6322441 is conventional basespace SRR6322441 read1 length is 150 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR6322441_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 150 %GC 55 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.7555 34.0 33.0 34.0 31.0 34.0 2 32.669 34.0 34.0 34.0 31.0 34.0 3 33.1855 34.0 34.0 34.0 31.0 34.0 4 36.621 37.0 37.0 37.0 35.0 37.0 5 36.69075 37.0 37.0 37.0 35.0 37.0 6 36.66825 37.0 37.0 37.0 37.0 37.0 7 36.616 37.0 37.0 37.0 36.0 37.0 8 36.65325 37.0 37.0 37.0 36.0 37.0 9 38.62375 39.0 39.0 39.0 38.0 39.0 10-14 38.9187 39.4 39.2 39.4 38.0 39.4 15-19 40.08335 41.0 40.0 41.0 38.2 41.0 20-24 39.9702 41.0 40.0 41.0 38.0 41.0 25-29 39.55055 41.0 39.8 41.0 37.0 41.0 30-34 39.2067 41.0 39.0 41.0 35.0 41.0 35-39 38.95085 41.0 38.6 41.0 35.0 41.0 40-44 38.4844 40.6 37.4 41.0 35.0 41.0 45-49 37.835 40.0 35.4 41.0 34.0 41.0 50-54 37.043949999999995 39.2 35.0 41.0 33.0 41.0 55-59 36.514300000000006 37.8 35.0 41.0 33.0 41.0 60-64 36.062599999999996 36.2 35.0 39.8 33.0 41.0 65-69 35.40235 35.0 35.0 38.8 32.8 41.0 70-74 34.739999999999995 35.0 35.0 36.8 32.4 39.4 75-79 33.84095 35.0 34.2 35.6 30.8 37.6 80-84 33.4649 35.0 34.0 35.0 30.8 36.4 85-89 33.08215 35.0 34.0 35.0 30.0 35.6 90-94 32.68325 35.0 33.2 35.0 29.2 35.0 95-99 32.339200000000005 35.0 33.2 35.0 27.8 35.0 100-104 31.924349999999997 35.0 33.0 35.0 25.8 35.0 105-109 31.645999999999997 35.0 33.0 35.0 24.4 35.0 110-114 30.98385 34.4 31.8 35.0 22.8 35.0 115-119 30.417150000000003 34.0 31.0 35.0 19.6 35.0 120-124 29.75355 34.0 29.8 35.0 15.6 35.0 125-129 29.23005 34.0 29.2 35.0 9.4 35.0 130-134 28.487350000000003 33.8 28.2 35.0 2.0 35.0 135-139 27.192 33.0 25.2 35.0 2.0 35.0 140-144 26.300900000000002 33.0 24.0 35.0 2.0 35.0 145-149 24.603699999999996 32.0 12.2 34.2 2.0 35.0 150 18.17125 23.0 2.0 30.0 2.0 33.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0 1101 2 0.0 1101 3 0.0 1101 4 0.0 1101 5 0.0 1101 6 0.0 1101 7 0.0 1101 8 0.0 1101 9 0.0 1101 10-14 0.0 1101 15-19 0.0 1101 20-24 0.0 1101 25-29 0.0 1101 30-34 0.0 1101 35-39 0.0 1101 40-44 0.0 1101 45-49 0.0 1101 50-54 0.0 1101 55-59 0.0 1101 60-64 0.0 1101 65-69 0.0 1101 70-74 0.0 1101 75-79 0.0 1101 80-84 0.0 1101 85-89 0.0 1101 90-94 0.0 1101 95-99 0.0 1101 100-104 0.0 1101 105-109 0.0 1101 110-114 0.0 1101 115-119 0.0 1101 120-124 0.0 1101 125-129 0.0 1101 130-134 0.0 1101 135-139 0.0 1101 140-144 0.0 1101 145-149 0.0 1101 150 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 1.0 4 1.0 5 3.0 6 2.0 7 5.0 8 8.0 9 8.0 10 6.0 11 3.0 12 6.0 13 8.0 14 4.0 15 8.0 16 13.0 17 7.0 18 17.0 19 20.0 20 17.0 21 19.0 22 26.0 23 24.0 24 31.0 25 39.0 26 38.0 27 65.0 28 64.0 29 92.0 30 99.0 31 127.0 32 193.0 33 264.0 34 395.0 35 683.0 36 1050.0 37 647.0 38 4.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 29.458179905312992 14.597580220936349 12.204103103629668 43.74013677012099 2 32.4 17.775 20.825 28.999999999999996 3 24.65 19.05 19.400000000000002 36.9 4 29.549999999999997 20.200000000000003 18.9 31.35 5 31.275 23.775 19.7 25.25 6 29.225 27.425 18.525 24.825 7 21.625 22.95 30.5 24.925 8 25.724999999999998 22.075 24.925 27.275 9 23.775 20.325 26.325 29.575000000000003 10-14 26.3 24.245 22.695 26.76 15-19 25.974999999999998 23.23 23.24 27.555000000000003 20-24 26.650000000000002 23.13 22.955000000000002 27.265 25-29 27.150000000000002 23.04 22.25 27.560000000000002 30-34 26.93 23.06 21.990000000000002 28.02 35-39 26.75 22.62 22.805 27.825 40-44 26.75 23.189999999999998 22.57 27.49 45-49 26.985 22.884999999999998 22.56 27.57 50-54 27.215 22.745 22.005 28.035 55-59 27.02 22.869999999999997 22.125 27.985 60-64 27.02 22.505 22.36 28.115000000000002 65-69 27.544999999999998 22.8 22.465 27.189999999999998 70-74 27.245 22.705000000000002 22.63 27.42 75-79 27.6 22.85 22.650000000000002 26.900000000000002 80-84 27.925 22.41 22.245 27.42 85-89 27.750000000000004 22.17 22.14 27.939999999999998 90-94 27.905 22.509999999999998 22.095000000000002 27.49 95-99 27.950000000000003 22.38 22.61 27.060000000000002 100-104 27.715 22.515 22.43 27.339999999999996 105-109 27.839999999999996 22.17 23.005 26.985 110-114 28.299999999999997 21.935 22.095000000000002 27.67 115-119 28.249999999999996 22.185 22.11 27.455000000000002 120-124 27.889999999999997 22.925 21.455 27.73 125-129 28.26 22.34 22.264999999999997 27.134999999999998 130-134 28.03 22.66 21.84 27.47 135-139 29.020000000000003 21.765 21.63 27.584999999999997 140-144 28.389999999999997 22.67 21.9 27.04 145-149 28.794999999999998 21.995 22.005 27.205000000000002 150 29.4 22.225 18.975 29.4 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.5 21 0.5 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.5 28 1.0 29 2.5 30 3.5 31 3.0 32 7.5 33 12.5 34 12.5 35 18.5 36 32.0 37 40.0 38 48.5 39 63.0 40 76.0 41 94.5 42 103.5 43 111.5 44 130.0 45 142.5 46 154.0 47 152.5 48 136.0 49 117.5 50 126.5 51 142.0 52 126.0 53 118.0 54 118.0 55 112.0 56 106.5 57 97.0 58 87.5 59 85.5 60 84.0 61 90.0 62 80.5 63 70.5 64 78.5 65 81.5 66 83.0 67 83.5 68 79.0 69 74.0 70 79.5 71 73.0 72 67.0 73 66.5 74 63.5 75 55.0 76 43.0 77 36.0 78 29.5 79 26.5 80 21.0 81 13.5 82 10.0 83 10.0 84 8.0 85 4.0 86 1.5 87 0.5 88 1.0 89 0.5 90 0.5 91 1.0 92 0.5 93 0.0 94 0.0 95 0.5 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 4.95 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 150 4000.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 99.725 #Duplication Level Percentage of deduplicated Percentage of total 1 99.72424166457759 99.45 2 0.2757583354224116 0.5499999999999999 3 0.0 0.0 4 0.0 0.0 5 0.0 0.0 6 0.0 0.0 7 0.0 0.0 8 0.0 0.0 9 0.0 0.0 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.0 0.0 0.0 0.0 0.0 70-71 0.0 0.0 0.0 0.0 0.0 72-73 0.0 0.0 0.0 0.0 0.0 74-75 0.0 0.0 0.0 0.0 0.0 76-77 0.0 0.0 0.0 0.0 0.0 78-79 0.0 0.0 0.0 0.0 0.0 80-81 0.0 0.0 0.0 0.0 0.0 82-83 0.0 0.0 0.0 0.0 0.0 84-85 0.0 0.0 0.0 0.0 0.0 86-87 0.0 0.0 0.0 0.0 0.0 88-89 0.0 0.0 0.0 0.0 0.0 90-91 0.0 0.0 0.0 0.0 0.0 92-93 0.0 0.0 0.0 0.0 0.0 94-95 0.0 0.0 0.0 0.0 0.0 96-97 0.0 0.0 0.0 0.0 0.0 98-99 0.0 0.0 0.0 0.0 0.0 100-101 0.0 0.0 0.0 0.0 0.0 102-103 0.0 0.0 0.0 0.0 0.0 104-105 0.025 0.0 0.0 0.0 0.0 106-107 0.1 0.0 0.0 0.0 0.0 108-109 0.1 0.0 0.0 0.0 0.0 110-111 0.15 0.0 0.0 0.0 0.0 112-113 0.15 0.0 0.0 0.0 0.0 114-115 0.15 0.0 0.0 0.0 0.0 116-117 0.3 0.0 0.0 0.0 0.0 118-119 0.3 0.0 0.0 0.0 0.0 120-121 0.3 0.0 0.0 0.0 0.0 122-123 0.4 0.0 0.0 0.0 0.0 124-125 0.45 0.0 0.0 0.0 0.0 126-127 0.5375000000000001 0.0 0.0 0.0 0.0 128-129 0.6125 0.0 0.0 0.0 0.0 130-131 0.7125 0.0 0.0 0.0 0.0 132-133 0.9750000000000001 0.0 0.0 0.0 0.0 134-135 1.25 0.0 0.0 0.0 0.0 136-137 1.4625 0.0 0.0 0.0 0.0 138 1.675 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content pass >>END_MODULE Read 2531419 spots for SRR6322441.sra Written 2531419 spots for SRR6322441.sra Read 2531419 spots for SRR6322441.sra Written 2531419 spots for SRR6322441.sra Read 2531419 spots for SRR6322441.sra Written 2531419 spots for SRR6322441.sra Read 2531419 spots for SRR6322441.sra Written 2531419 spots for SRR6322441.sra Read 2531419 spots for SRR6322441.sra Written 2531419 spots for SRR6322441.sra Read 2531419 spots for SRR6322441.sra Written 2531419 spots for SRR6322441.sra Read 2531419 spots for SRR6322441.sra Written 2531419 spots for SRR6322441.sra Read 2531423 spots for SRR6322441.sra Written 2531423 spots for SRR6322441.sra Read 2531419 spots for SRR6322441.sra Written 2531419 spots for SRR6322441.sra Read 2531419 spots for SRR6322441.sra Written 2531419 spots for SRR6322441.sra Read 2531419 spots for SRR6322441.sra Written 2531419 spots for SRR6322441.sra Read 2531419 spots for SRR6322441.sra Written 2531419 spots for SRR6322441.sra Read 2531419 spots for SRR6322441.sra Written 2531419 spots for SRR6322441.sra Read 2531419 spots for SRR6322441.sra Written 2531419 spots for SRR6322441.sra Read 2531419 spots for SRR6322441.sra Written 2531419 spots for SRR6322441.sra Read 2531419 spots for SRR6322441.sra Written 2531419 spots for SRR6322441.sra Read 2531419 spots for SRR6322441.sra Written 2531419 spots for SRR6322441.sra Read 2531419 spots for SRR6322441.sra Written 2531419 spots for SRR6322441.sra Read 2531419 spots for SRR6322441.sra Written 2531419 spots for SRR6322441.sra Read 2531419 spots for SRR6322441.sra Written 2531419 spots for SRR6322441.sra SRR ids: ['SRR6322441.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_wj6ewz7d SRR6322441.sra spots: 50628384 blocks: [[1, 2531419], [2531420, 5062838], [5062839, 7594257], [7594258, 10125676], [10125677, 12657095], [12657096, 15188514], [15188515, 17719933], [17719934, 20251352], [20251353, 22782771], [22782772, 25314190], [25314191, 27845609], [27845610, 30377028], [30377029, 32908447], [32908448, 35439866], [35439867, 37971285], [37971286, 40502704], [40502705, 43034123], [43034124, 45565542], [45565543, 48096961], [48096962, 50628384]] SRR6322441 file size 18630194 SRR6322441 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR6322441 SRR6322441_1.fastq Input file: SRR6322441_1.fastq trimmed: SRR6322441-trimmed.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- minimum overlap length for adapter detection (-k): inf -- number of concurrent threads (-t): 20 Sat Dec 7 10:59:58 2024 >> started Sat Dec 7 11:00:30 2024 >> done (32.542s) 50628384 reads processed; of these: 63398 ( 0.13%) short reads filtered out after trimming by size control 50694 ( 0.10%) empty reads filtered out after trimming by size control 50514292 (99.77%) reads available; of these: 26616442 (52.69%) trimmed reads available after processing 23897850 (47.31%) untrimmed reads available after processing Length distribution of reads after trimming: length count percentage 18 7117 0.01% 19 7561 0.01% 20 8482 0.02% 21 8664 0.02% 22 9102 0.02% 23 10265 0.02% 24 11194 0.02% 25 12060 0.02% 26 14517 0.03% 27 13321 0.03% 28 13562 0.03% 29 14656 0.03% 30 14312 0.03% 31 14966 0.03% 32 15401 0.03% 33 16185 0.03% 34 16497 0.03% 35 16798 0.03% 36 16734 0.03% 37 17615 0.03% 38 17652 0.03% 39 18979 0.04% 40 18807 0.04% 41 19731 0.04% 42 20443 0.04% 43 20845 0.04% 44 20974 0.04% 45 21121 0.04% 46 22880 0.05% 47 23756 0.05% 48 23848 0.05% 49 23269 0.05% 50 24163 0.05% 51 20863 0.04% 52 20130 0.04% 53 21523 0.04% 54 21933 0.04% 55 22356 0.04% 56 22517 0.04% 57 23020 0.05% 58 22881 0.05% 59 22956 0.05% 60 23625 0.05% 61 25033 0.05% 62 26068 0.05% 63 26889 0.05% 64 28504 0.06% 65 33388 0.07% 66 33739 0.07% 67 33156 0.07% 68 33737 0.07% 69 33838 0.07% 70 34282 0.07% 71 37216 0.07% 72 39090 0.08% 73 40734 0.08% 74 41890 0.08% 75 41762 0.08% 76 37115 0.07% 77 39633 0.08% 78 44201 0.09% 79 46182 0.09% 80 49174 0.10% 81 53749 0.11% 82 54736 0.11% 83 56781 0.11% 84 59669 0.12% 85 62340 0.12% 86 64558 0.13% 87 67507 0.13% 88 68294 0.14% 89 70971 0.14% 90 75035 0.15% 91 76912 0.15% 92 79646 0.16% 93 83384 0.17% 94 87881 0.17% 95 91867 0.18% 96 96185 0.19% 97 99840 0.20% 98 104794 0.21% 99 108221 0.21% 100 112697 0.22% 101 118140 0.23% 102 122971 0.24% 103 126853 0.25% 104 130904 0.26% 105 137872 0.27% 106 142510 0.28% 107 148133 0.29% 108 152985 0.30% 109 159163 0.32% 110 169138 0.33% 111 175545 0.35% 112 184030 0.36% 113 192704 0.38% 114 206012 0.41% 115 221496 0.44% 116 236200 0.47% 117 239942 0.47% 118 235074 0.47% 119 236283 0.47% 120 242740 0.48% 121 246574 0.49% 122 258768 0.51% 123 262309 0.52% 124 270742 0.54% 125 282272 0.56% 126 288686 0.57% 127 296634 0.59% 128 314611 0.62% 129 327335 0.65% 130 341885 0.68% 131 362592 0.72% 132 377026 0.75% 133 399868 0.79% 134 421399 0.83% 135 431003 0.85% 136 444404 0.88% 137 468615 0.93% 138 491371 0.97% 139 526754 1.04% 140 575819 1.14% 141 622192 1.23% 142 690593 1.37% 143 777618 1.54% 144 876083 1.73% 145 1019721 2.02% 146 1231412 2.44% 147 1468758 2.91% 148 1870978 3.70% 149 4033746 7.99% 150 23897850 47.31% 50514292 reads passed initial QC criterion=sequence-density sequence-density=1.26 sequence-density-rank=1 fanout-score=71.19 fanout-score-rank=9 prefix-density=1.90 prefix-fanout=47.1 sequence=AGATCGGAAGAGC criterion=fanout-score sequence-density=0.02 sequence-density-rank=31 fanout-score=1456.37 fanout-score-rank=1 prefix-density=1.16 prefix-fanout=27.5 sequence=GCGGCGGCGAACCGCCCCCGTCGCCGCGATCCGAACACTTCACCGGACCATTCAATCGGTAGGAGCGACGGGCGGTGTGTACAAAGGGCAGGGACGTAGTCAACGCGAGCTGATGACTCGCGCTTACTAGGCATTCCTCGTTGAAGACCAACAATTGCAATGATCTATCCCCATCACGATGAAATTTCCCAAGATTACCCGGGCCTGTCGGCCAAGGCTATATACTCGTTGAATACATCAGTGTAGCGCGCGTGCGGCCCAGAACATCTAAGGGCATCACAGACCTGTTATTGCCTCAAACTTCCGTCGCCTAAACGGCGATAGTCCCTCTAAGAAGCTAGCTGCGGAGGGATGGCTCCGCATAGCTAGTTAGCAGGCTGAGGTCTCGTTCGTTAACGGAATTAACCAGACAAATCGCTCCACCAACTAAGAACGGCCATGCACCACCACCCATAGAATCAAGAAAGAGCTCTCAGTCTGTCAATCCTTGCTATGTCTGGACCTGGTAAGT Started job on | Dec 07 11:00:51 Started mapping on | Dec 07 11:00:51 Finished on | Dec 07 11:02:05 Mapping speed, Million of reads per hour | 2457.45 Number of input reads | 50514292 Average input read length | 139 UNIQUE READS: Uniquely mapped reads number | 47816812 Uniquely mapped reads % | 94.66% Average mapped length | 138.58 Number of splices: Total | 18766654 Number of splices: Annotated (sjdb) | 17714585 Number of splices: GT/AG | 18509861 Number of splices: GC/AG | 205627 Number of splices: AT/AC | 11472 Number of splices: Non-canonical | 39694 Mismatch rate per base, % | 0.28% Deletion rate per base | 0.02% Deletion average length | 2.70 Insertion rate per base | 0.01% Insertion average length | 2.32 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 946044 % of reads mapped to multiple loci | 1.87% Number of reads mapped to too many loci | 1287724 % of reads mapped to too many loci | 2.55% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 0.60% % of reads unmapped: other | 0.31% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 1751436 1751436 1751436 N_multimapping 946044 946044 946044 N_noFeature 1223776 24256009 24146553 N_ambiguous 718178 42931 45762 UnstrandedReadsAssigned:45874858 PositiveStrandReadsAssigned:23517872 NegativeStrandReadsAssigned:23624497 Dataset is classified unstranded MeadianReadLen=150 20thPercentileLength=143 echo kmer=139 SRR6322441 Starting Kallisto single end mapping to ensembl reference transcriptome. kmer=31 [quant] fragment length distribution is truncated gaussian with mean = 100, sd = 20 [index] k-mer length: 31 [index] number of targets: 52,972 [index] number of k-mers: 66,720,672 [index] number of equivalence classes: 111,837 [quant] running in single-end mode [quant] will process file 1: SRR6322441-trimmed.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 50,514,292 reads, 47,117,578 reads pseudoaligned [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,267 rounds 52973 SRR6322441.ke.tsv 35125 SRR6322441.se.tsv 88098 total ==> SRR6322441.ke.tsv <== target_id length eff_length est_counts tpm PNS24245 936 837 2.74578e-06 1.11705e-07 PNS24247 1044 945 73.3413 2.64272 PNS24249 1928 1829 790.532 14.7177 PNS24246 1044 945 73.3413 2.64272 PNS24248 1044 945 73.3413 2.64272 PNS24244 1471 1372 98.4446 2.44327 PNS24243 293 194 18 3.1594 KQK14069 1603 1504 3155.31 71.4378 KQK14071 474 375 566.065 51.4007 ==> SRR6322441.se.tsv <== BRADI_1g14170v3 3786 BRADI_1g53295v3 375 BRADI_1g59795v3 662 BRADI_1g07683v3 0 BRADI_1g00485v3 32 BRADI_1g20270v3 1166 BRADI_1g74790v3 835 BRADI_1g09890v3 0 BRADI_1g77505v3 394 BRADI_1g48960v3 19 SRR6322441 completed mapping pipeline successfully