Starting /dee2/code/volunteer_pipeline.sh SRR6322442
    current disk space = 1543162208256
    free memory = 1598930948 
SRR6322442 SRAfilesize
736ed6b0077d90dfe7a55d59047c93f6  SRR6322442.sra
SRR6322442.sra file validated
SRR6322442 is single end
SRR6322442 is conventional basespace
SRR6322442 read1 length is 150 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR6322442_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	150
%GC	55
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.69925	34.0	33.0	34.0	31.0	34.0
2	32.59075	34.0	34.0	34.0	31.0	34.0
3	33.122	34.0	34.0	34.0	31.0	34.0
4	36.591	37.0	37.0	37.0	35.0	37.0
5	36.68575	37.0	37.0	37.0	37.0	37.0
6	36.675	37.0	37.0	37.0	37.0	37.0
7	36.64	37.0	37.0	37.0	36.0	37.0
8	36.6695	37.0	37.0	37.0	37.0	37.0
9	38.61875	39.0	39.0	39.0	38.0	39.0
10-14	38.9204	39.4	39.2	39.4	38.2	39.4
15-19	40.072	41.0	40.0	41.0	38.2	41.0
20-24	39.96445	41.0	40.0	41.0	38.0	41.0
25-29	39.54835	41.0	39.8	41.0	37.0	41.0
30-34	39.188599999999994	40.8	38.8	41.0	35.4	41.0
35-39	38.88035	41.0	38.6	41.0	35.0	41.0
40-44	38.362700000000004	40.0	36.8	41.0	35.0	41.0
45-49	37.7236	39.8	35.0	41.0	33.6	41.0
50-54	36.91674999999999	38.6	35.0	40.6	33.0	41.0
55-59	36.398300000000006	37.2	35.0	40.6	32.8	41.0
60-64	35.952600000000004	35.8	35.0	39.8	33.0	41.0
65-69	35.31705	35.0	35.0	38.4	32.6	41.0
70-74	34.670249999999996	35.0	35.0	36.8	32.2	39.4
75-79	33.80845000000001	35.0	34.2	35.4	30.8	37.4
80-84	33.37955	35.0	34.0	35.0	30.2	36.4
85-89	33.07115	35.0	34.0	35.0	30.0	35.8
90-94	32.6579	35.0	33.4	35.0	29.0	35.0
95-99	32.34745	35.0	33.0	35.0	28.2	35.0
100-104	31.870000000000005	35.0	33.0	35.0	25.4	35.0
105-109	31.534399999999998	35.0	32.8	35.0	24.4	35.0
110-114	30.861699999999995	34.6	31.4	35.0	21.4	35.0
115-119	30.37575	34.0	31.0	35.0	19.4	35.0
120-124	29.517200000000003	34.0	29.4	35.0	10.8	35.0
125-129	28.84865	34.0	29.0	35.0	3.0	35.0
130-134	28.040099999999995	33.6	27.8	35.0	2.0	35.0
135-139	26.85605	33.0	24.8	35.0	2.0	35.0
140-144	26.018600000000003	33.0	23.2	35.0	2.0	35.0
145-149	24.36995	32.0	11.0	34.0	2.0	35.0
150	17.77275	23.0	2.0	30.0	2.0	34.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.0
1101	2	0.0
1101	3	0.0
1101	4	0.0
1101	5	0.0
1101	6	0.0
1101	7	0.0
1101	8	0.0
1101	9	0.0
1101	10-14	0.0
1101	15-19	0.0
1101	20-24	0.0
1101	25-29	0.0
1101	30-34	0.0
1101	35-39	0.0
1101	40-44	0.0
1101	45-49	0.0
1101	50-54	0.0
1101	55-59	0.0
1101	60-64	0.0
1101	65-69	0.0
1101	70-74	0.0
1101	75-79	0.0
1101	80-84	0.0
1101	85-89	0.0
1101	90-94	0.0
1101	95-99	0.0
1101	100-104	0.0
1101	105-109	0.0
1101	110-114	0.0
1101	115-119	0.0
1101	120-124	0.0
1101	125-129	0.0
1101	130-134	0.0
1101	135-139	0.0
1101	140-144	0.0
1101	145-149	0.0
1101	150	0.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	5.0
3	0.0
4	1.0
5	4.0
6	2.0
7	2.0
8	10.0
9	4.0
10	7.0
11	7.0
12	4.0
13	5.0
14	4.0
15	10.0
16	11.0
17	13.0
18	11.0
19	17.0
20	17.0
21	27.0
22	24.0
23	32.0
24	35.0
25	46.0
26	56.0
27	62.0
28	70.0
29	91.0
30	106.0
31	126.0
32	200.0
33	244.0
34	393.0
35	684.0
36	1028.0
37	641.0
38	1.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	30.139878595935603	14.119820533122196	11.797307996832936	43.942992874109265
2	32.96648324162081	16.783391695847925	19.634817408704354	30.61530765382691
3	25.1	19.225	18.55	37.125
4	29.299999999999997	20.575	17.549999999999997	32.574999999999996
5	31.324999999999996	23.575	20.150000000000002	24.95
6	29.15	26.375	19.275000000000002	25.2
7	23.275000000000002	23.5	30.725	22.5
8	27.675	21.475	23.05	27.800000000000004
9	26.35	20.474999999999998	25.85	27.325
10-14	26.875	24.235	21.925	26.965
15-19	27.255000000000003	22.830000000000002	22.33	27.584999999999997
20-24	27.950000000000003	23.305	21.68	27.065
25-29	27.13	22.8	22.05	28.02
30-34	27.325	22.18	22.2	28.294999999999998
35-39	27.405	22.900000000000002	22.1	27.595
40-44	27.785	22.770000000000003	21.69	27.755000000000003
45-49	27.26	23.095	22.065	27.58
50-54	28.050000000000004	22.3	22.025	27.625
55-59	27.794999999999998	22.435	22.205	27.565
60-64	27.275	22.615	22.585	27.525
65-69	27.555000000000003	22.189999999999998	22.435	27.82
70-74	27.57	22.355	22.03	28.044999999999998
75-79	27.474999999999998	22.55	21.82	28.155
80-84	27.944999999999997	22.915	21.654999999999998	27.485
85-89	27.500000000000004	22.225	21.93	28.345
90-94	27.915	22.125	22.025	27.935
95-99	28.215	22.33	21.884999999999998	27.57
100-104	28.46	21.81	22.040000000000003	27.689999999999998
105-109	28.185	21.95	22.134999999999998	27.73
110-114	28.13	22.33	22.105	27.435
115-119	28.305000000000003	22.2	21.834999999999997	27.66
120-124	28.275	22.29	21.595	27.839999999999996
125-129	28.68	22.23	21.865000000000002	27.224999999999998
130-134	28.615000000000002	22.31	21.505	27.57
135-139	28.515	22.18	21.55	27.755000000000003
140-144	28.78	21.89	21.8	27.529999999999998
145-149	28.965000000000003	21.98	21.6	27.455000000000002
150	30.675	20.05	18.9	30.375000000000004
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1.0
1	0.5
2	0.5
3	0.5
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.5
17	0.5
18	0.0
19	0.0
20	0.0
21	0.0
22	0.5
23	1.0
24	1.0
25	0.5
26	0.5
27	1.0
28	1.0
29	1.5
30	2.0
31	2.5
32	4.0
33	6.0
34	12.5
35	14.5
36	17.5
37	30.5
38	45.5
39	55.5
40	63.5
41	86.0
42	109.0
43	115.0
44	127.5
45	140.5
46	151.0
47	150.5
48	129.0
49	121.0
50	122.5
51	129.5
52	132.0
53	121.5
54	103.0
55	97.5
56	108.0
57	98.0
58	84.0
59	84.5
60	88.5
61	87.5
62	82.5
63	82.5
64	84.5
65	92.5
66	96.5
67	89.5
68	83.0
69	86.0
70	88.5
71	87.0
72	77.5
73	70.5
74	70.0
75	57.0
76	43.5
77	36.0
78	30.0
79	26.0
80	18.0
81	10.5
82	10.0
83	9.5
84	6.5
85	3.0
86	2.5
87	1.5
88	1.0
89	1.0
90	1.0
91	0.5
92	0.5
93	1.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	warn
#Base	N-Count
1	5.2749999999999995
2	0.05
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
150	4000.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	99.85000000000001
#Duplication Level	Percentage of deduplicated	Percentage of total
1	99.84977466199298	99.7
2	0.15022533800701052	0.3
3	0.0	0.0
4	0.0	0.0
5	0.0	0.0
6	0.0	0.0
7	0.0	0.0
8	0.0	0.0
9	0.0	0.0
>10	0.0	0.0
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	0.0	0.0
20-21	0.0	0.0	0.0	0.0	0.0
22-23	0.0	0.0	0.0	0.0	0.0
24-25	0.0	0.0	0.0	0.0	0.0
26-27	0.0	0.0	0.0	0.0	0.0
28-29	0.0	0.0	0.0	0.0	0.0
30-31	0.0	0.0	0.0	0.0	0.0
32-33	0.0	0.0	0.0	0.0	0.0
34-35	0.0	0.0	0.0	0.0	0.0
36-37	0.0	0.0	0.0	0.0	0.0
38-39	0.0	0.0	0.0	0.0	0.0
40-41	0.0	0.0	0.0	0.0	0.0
42-43	0.0	0.0	0.0	0.0	0.0
44-45	0.0	0.0	0.0	0.0	0.0
46-47	0.0	0.0	0.0	0.0	0.0
48-49	0.0	0.0	0.0	0.0	0.0
50-51	0.0	0.0	0.0	0.0	0.0
52-53	0.0	0.0	0.0	0.0	0.0
54-55	0.0	0.0	0.0	0.0	0.0
56-57	0.0	0.0	0.0	0.0	0.0
58-59	0.0	0.0	0.0	0.0	0.0
60-61	0.0	0.0	0.0	0.0	0.0
62-63	0.0	0.0	0.0	0.0	0.0
64-65	0.0	0.0	0.0	0.0	0.0
66-67	0.0	0.0	0.0	0.0	0.0
68-69	0.0	0.0	0.0	0.0	0.0
70-71	0.0	0.0	0.0	0.0	0.0
72-73	0.0	0.0	0.0	0.0	0.0
74-75	0.0	0.0	0.0	0.0	0.0
76-77	0.0	0.0	0.0	0.0	0.0
78-79	0.0	0.0	0.0	0.0	0.0
80-81	0.0	0.0	0.0	0.0	0.0
82-83	0.0	0.0	0.0	0.0	0.0
84-85	0.0	0.0	0.0	0.0	0.0
86-87	0.0	0.0	0.0	0.0	0.0
88-89	0.0	0.0	0.0	0.0	0.0
90-91	0.0	0.0	0.0	0.0	0.0
92-93	0.0	0.0	0.0	0.0	0.0
94-95	0.0	0.0	0.0	0.0	0.0
96-97	0.0	0.0	0.0	0.0	0.0
98-99	0.0	0.0	0.0	0.0	0.0
100-101	0.0	0.0	0.0	0.0	0.0
102-103	0.0	0.0	0.0	0.0	0.0
104-105	0.0	0.0	0.0	0.0	0.0
106-107	0.0	0.0	0.0	0.0	0.0
108-109	0.0	0.0	0.0	0.0	0.0
110-111	0.0	0.0	0.0	0.0	0.0
112-113	0.05	0.0	0.0	0.0	0.0
114-115	0.05	0.0	0.0	0.0	0.0
116-117	0.07500000000000001	0.0	0.0	0.0	0.0
118-119	0.1	0.0	0.0	0.0	0.0
120-121	0.1	0.0	0.0	0.0	0.0
122-123	0.1125	0.0	0.0	0.0	0.0
124-125	0.175	0.0	0.0	0.0	0.0
126-127	0.225	0.0	0.0	0.0	0.0
128-129	0.275	0.0	0.0	0.0	0.0
130-131	0.7	0.0	0.0	0.0	0.0
132-133	0.975	0.0	0.0	0.0	0.0
134-135	1.175	0.0	0.0	0.0	0.0
136-137	1.4500000000000002	0.0	0.0	0.0	0.0
138	1.825	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTCCAA	10	0.006973645	144.0	6
>>END_MODULE
Read 2436904 spots for SRR6322442.sra
Written 2436904 spots for SRR6322442.sra
Read 2436890 spots for SRR6322442.sra
Written 2436890 spots for SRR6322442.sra
Read 2436890 spots for SRR6322442.sra
Written 2436890 spots for SRR6322442.sra
Read 2436890 spots for SRR6322442.sra
Written 2436890 spots for SRR6322442.sra
Read 2436890 spots for SRR6322442.sra
Written 2436890 spots for SRR6322442.sra
Read 2436890 spots for SRR6322442.sra
Written 2436890 spots for SRR6322442.sra
Read 2436890 spots for SRR6322442.sra
Written 2436890 spots for SRR6322442.sra
Read 2436890 spots for SRR6322442.sra
Written 2436890 spots for SRR6322442.sra
Read 2436890 spots for SRR6322442.sra
Written 2436890 spots for SRR6322442.sra
Read 2436890 spots for SRR6322442.sra
Written 2436890 spots for SRR6322442.sra
Read 2436890 spots for SRR6322442.sra
Written 2436890 spots for SRR6322442.sra
Read 2436890 spots for SRR6322442.sra
Written 2436890 spots for SRR6322442.sra
Read 2436890 spots for SRR6322442.sra
Written 2436890 spots for SRR6322442.sra
Read 2436890 spots for SRR6322442.sra
Written 2436890 spots for SRR6322442.sra
Read 2436890 spots for SRR6322442.sra
Written 2436890 spots for SRR6322442.sra
Read 2436890 spots for SRR6322442.sra
Written 2436890 spots for SRR6322442.sra
Read 2436890 spots for SRR6322442.sra
Written 2436890 spots for SRR6322442.sra
Read 2436890 spots for SRR6322442.sra
Written 2436890 spots for SRR6322442.sra
Read 2436890 spots for SRR6322442.sra
Written 2436890 spots for SRR6322442.sra
Read 2436890 spots for SRR6322442.sra
Written 2436890 spots for SRR6322442.sra
SRR ids: ['SRR6322442.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_8j40vzjq
SRR6322442.sra spots: 48737814
blocks: [[1, 2436890], [2436891, 4873780], [4873781, 7310670], [7310671, 9747560], [9747561, 12184450], [12184451, 14621340], [14621341, 17058230], [17058231, 19495120], [19495121, 21932010], [21932011, 24368900], [24368901, 26805790], [26805791, 29242680], [29242681, 31679570], [31679571, 34116460], [34116461, 36553350], [36553351, 38990240], [38990241, 41427130], [41427131, 43864020], [43864021, 46300910], [46300911, 48737814]]
SRR6322442 file size 17934112
SRR6322442 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR6322442 SRR6322442_1.fastq
Input file:	SRR6322442_1.fastq
trimmed:	SRR6322442-trimmed.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- minimum overlap length for adapter detection (-k):	inf
-- number of concurrent threads (-t):	20
Sat Dec  7 12:31:24 2024 >> started

Sat Dec  7 12:31:58 2024 >> done (34.389s)
48737814 reads processed; of these:
   57727 ( 0.12%) short reads filtered out after trimming by size control
   43646 ( 0.09%) empty reads filtered out after trimming by size control
48636441 (99.79%) reads available; of these:
25557027 (52.55%) trimmed reads available after processing
23079414 (47.45%) untrimmed reads available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	    6470	  0.01%
 19	    7289	  0.01%
 20	    7795	  0.02%
 21	    8259	  0.02%
 22	    8451	  0.02%
 23	    9265	  0.02%
 24	   10028	  0.02%
 25	   11253	  0.02%
 26	   13121	  0.03%
 27	   12298	  0.03%
 28	   12530	  0.03%
 29	   13566	  0.03%
 30	   13291	  0.03%
 31	   13968	  0.03%
 32	   14676	  0.03%
 33	   14736	  0.03%
 34	   14988	  0.03%
 35	   15398	  0.03%
 36	   15736	  0.03%
 37	   16145	  0.03%
 38	   16104	  0.03%
 39	   17419	  0.04%
 40	   17417	  0.04%
 41	   18024	  0.04%
 42	   18694	  0.04%
 43	   19572	  0.04%
 44	   19257	  0.04%
 45	   19582	  0.04%
 46	   20717	  0.04%
 47	   21741	  0.04%
 48	   22329	  0.05%
 49	   22197	  0.05%
 50	   22279	  0.05%
 51	   19018	  0.04%
 52	   18806	  0.04%
 53	   20221	  0.04%
 54	   20286	  0.04%
 55	   20815	  0.04%
 56	   21236	  0.04%
 57	   21529	  0.04%
 58	   21132	  0.04%
 59	   21321	  0.04%
 60	   22268	  0.05%
 61	   23200	  0.05%
 62	   24261	  0.05%
 63	   25042	  0.05%
 64	   26601	  0.05%
 65	   31041	  0.06%
 66	   30963	  0.06%
 67	   30951	  0.06%
 68	   31804	  0.07%
 69	   31820	  0.07%
 70	   32439	  0.07%
 71	   34745	  0.07%
 72	   37090	  0.08%
 73	   38387	  0.08%
 74	   39358	  0.08%
 75	   39771	  0.08%
 76	   34787	  0.07%
 77	   36975	  0.08%
 78	   41651	  0.09%
 79	   42933	  0.09%
 80	   46144	  0.09%
 81	   50306	  0.10%
 82	   51609	  0.11%
 83	   53847	  0.11%
 84	   56234	  0.12%
 85	   59395	  0.12%
 86	   60336	  0.12%
 87	   63630	  0.13%
 88	   64946	  0.13%
 89	   67470	  0.14%
 90	   70251	  0.14%
 91	   72589	  0.15%
 92	   75586	  0.16%
 93	   79180	  0.16%
 94	   82840	  0.17%
 95	   87223	  0.18%
 96	   91081	  0.19%
 97	   94669	  0.19%
 98	   99624	  0.20%
 99	  102752	  0.21%
100	  106756	  0.22%
101	  112917	  0.23%
102	  117333	  0.24%
103	  120781	  0.25%
104	  126464	  0.26%
105	  132109	  0.27%
106	  135070	  0.28%
107	  141351	  0.29%
108	  145122	  0.30%
109	  151529	  0.31%
110	  162033	  0.33%
111	  168851	  0.35%
112	  177435	  0.36%
113	  186446	  0.38%
114	  198686	  0.41%
115	  211989	  0.44%
116	  226401	  0.47%
117	  229472	  0.47%
118	  226110	  0.46%
119	  228965	  0.47%
120	  233684	  0.48%
121	  237574	  0.49%
122	  247083	  0.51%
123	  252346	  0.52%
124	  260216	  0.54%
125	  272149	  0.56%
126	  277184	  0.57%
127	  285035	  0.59%
128	  303623	  0.62%
129	  314459	  0.65%
130	  328923	  0.68%
131	  349252	  0.72%
132	  363814	  0.75%
133	  385646	  0.79%
134	  406540	  0.84%
135	  414702	  0.85%
136	  426391	  0.88%
137	  450607	  0.93%
138	  474259	  0.98%
139	  505836	  1.04%
140	  554753	  1.14%
141	  601539	  1.24%
142	  667188	  1.37%
143	  748190	  1.54%
144	  844603	  1.74%
145	  982773	  2.02%
146	 1188587	  2.44%
147	 1416496	  2.91%
148	 1804432	  3.71%
149	 3888565	  8.00%
150	23079414	 47.45%
48636441 reads passed initial QC


criterion=sequence-density
sequence-density=1.26
sequence-density-rank=1
fanout-score=72.86
fanout-score-rank=9
prefix-density=1.94
prefix-fanout=47.3
sequence=AGATCGGAAGAGC


criterion=fanout-score
sequence-density=0.10
sequence-density-rank=16
fanout-score=320.11
fanout-score-rank=1
prefix-density=1.21
prefix-fanout=27.6
sequence=CGGCGGCGGCGCC
                                 Started job on |	Dec 07 12:32:21
                             Started mapping on |	Dec 07 12:32:21
                                    Finished on |	Dec 07 12:33:28
       Mapping speed, Million of reads per hour |	2613.30

                          Number of input reads |	48636441
                      Average input read length |	139
                                    UNIQUE READS:
                   Uniquely mapped reads number |	46095126
                        Uniquely mapped reads % |	94.77%
                          Average mapped length |	138.70
                       Number of splices: Total |	18319599
            Number of splices: Annotated (sjdb) |	17325058
                       Number of splices: GT/AG |	18066621
                       Number of splices: GC/AG |	202461
                       Number of splices: AT/AC |	11498
               Number of splices: Non-canonical |	39019
                      Mismatch rate per base, % |	0.29%
                         Deletion rate per base |	0.02%
                        Deletion average length |	2.38
                        Insertion rate per base |	0.01%
                       Insertion average length |	2.28
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	838139
             % of reads mapped to multiple loci |	1.72%
        Number of reads mapped to too many loci |	1243046
             % of reads mapped to too many loci |	2.56%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	0.61%
                     % of reads unmapped: other |	0.34%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	1703176	1703176	1703176
N_multimapping	838139	838139	838139
N_noFeature	1111397	23338840	23247605
N_ambiguous	705264	45988	48437
UnstrandedReadsAssigned:44278465 PositiveStrandReadsAssigned:22710298 NegativeStrandReadsAssigned:22799084
Dataset is classified unstranded
MeadianReadLen=150 20thPercentileLength=143 echo kmer=139
SRR6322442 Starting Kallisto single end mapping to ensembl reference transcriptome. kmer=31

[quant] fragment length distribution is truncated gaussian with mean = 100, sd = 20
[index] k-mer length: 31
[index] number of targets: 52,972
[index] number of k-mers: 66,720,672
[index] number of equivalence classes: 111,837
[quant] running in single-end mode
[quant] will process file 1: SRR6322442-trimmed.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 48,636,441 reads, 45,514,044 reads pseudoaligned
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 1,301 rounds

  52973 SRR6322442.ke.tsv
  35125 SRR6322442.se.tsv
  88098 total
==> SRR6322442.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
PNS24245	936	837	0	0
PNS24247	1044	945	74.584	2.7786
PNS24249	1928	1829	949.496	18.2765
PNS24246	1044	945	74.584	2.7786
PNS24248	1044	945	74.584	2.7786
PNS24244	1471	1372	79.7517	2.04644
PNS24243	293	194	17	3.08503
KQK14069	1603	1504	15016.8	351.514
KQK14071	474	375	3115.53	292.491

==> SRR6322442.se.tsv <==
BRADI_1g14170v3	18561
BRADI_1g53295v3	503
BRADI_1g59795v3	467
BRADI_1g07683v3	0
BRADI_1g00485v3	49
BRADI_1g20270v3	997
BRADI_1g74790v3	855
BRADI_1g09890v3	0
BRADI_1g77505v3	391
BRADI_1g48960v3	0
SRR6322442 completed mapping pipeline successfully
