Starting /dee2/code/volunteer_pipeline.sh SRR6322443 current disk space = 1543341838336 free memory = 1606407612 SRR6322443 SRAfilesize 2d7f4c78e70a0f338ecd9bc416c41328 SRR6322443.sra SRR6322443.sra file validated SRR6322443 is single end SRR6322443 is conventional basespace SRR6322443 read1 length is 150 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR6322443_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 150 %GC 55 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.13975 34.0 33.0 34.0 31.0 34.0 2 32.8655 34.0 34.0 34.0 31.0 34.0 3 33.288 34.0 34.0 34.0 31.0 34.0 4 36.5835 37.0 37.0 37.0 35.0 37.0 5 36.69375 37.0 37.0 37.0 35.0 37.0 6 36.7265 37.0 37.0 37.0 37.0 37.0 7 36.752 37.0 37.0 37.0 37.0 37.0 8 36.72775 37.0 37.0 37.0 37.0 37.0 9 38.61375 39.0 39.0 39.0 38.0 39.0 10-14 38.92530000000001 39.4 39.2 39.4 38.4 39.4 15-19 40.19295 41.0 40.0 41.0 38.6 41.0 20-24 39.9841 41.0 40.0 41.0 38.0 41.0 25-29 39.722500000000004 41.0 40.0 41.0 37.6 41.0 30-34 39.321749999999994 41.0 39.4 41.0 35.8 41.0 35-39 39.033550000000005 41.0 38.6 41.0 35.0 41.0 40-44 38.54005 40.4 37.4 41.0 35.0 41.0 45-49 37.928200000000004 40.0 35.4 41.0 33.8 41.0 50-54 37.1887 39.2 35.0 40.6 33.0 41.0 55-59 36.53385000000001 37.4 35.0 40.6 32.8 41.0 60-64 36.040499999999994 35.8 35.0 39.8 33.0 41.0 65-69 35.483399999999996 35.0 35.0 38.8 32.8 40.8 70-74 34.68835 35.0 35.0 36.8 31.6 39.2 75-79 33.81125 35.0 33.8 35.6 30.4 37.4 80-84 33.314800000000005 35.0 34.0 35.0 30.2 36.2 85-89 32.67075 35.0 33.2 35.0 28.2 35.4 90-94 32.20115 35.0 33.0 35.0 27.0 35.0 95-99 31.898900000000005 35.0 32.6 35.0 25.8 35.0 100-104 31.4557 34.8 32.0 35.0 24.4 35.0 105-109 30.950499999999998 34.0 31.0 35.0 23.2 35.0 110-114 30.196949999999998 34.0 30.0 35.0 19.2 35.0 115-119 29.512899999999995 34.0 29.2 35.0 15.8 35.0 120-124 28.64745 33.6 28.2 35.0 6.2 35.0 125-129 27.51925 33.0 25.8 35.0 2.0 35.0 130-134 26.54305 32.4 24.0 34.8 2.0 35.0 135-139 25.194 31.4 20.0 34.0 2.0 35.0 140-144 23.770000000000003 30.8 10.2 34.0 2.0 35.0 145-149 21.8187 30.2 2.0 34.0 2.0 35.0 150 14.9635 17.0 2.0 27.0 2.0 32.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0 1101 2 0.0 1101 3 0.0 1101 4 0.0 1101 5 0.0 1101 6 0.0 1101 7 0.0 1101 8 0.0 1101 9 0.0 1101 10-14 0.0 1101 15-19 0.0 1101 20-24 0.0 1101 25-29 0.0 1101 30-34 0.0 1101 35-39 0.0 1101 40-44 0.0 1101 45-49 0.0 1101 50-54 0.0 1101 55-59 0.0 1101 60-64 0.0 1101 65-69 0.0 1101 70-74 0.0 1101 75-79 0.0 1101 80-84 0.0 1101 85-89 0.0 1101 90-94 0.0 1101 95-99 0.0 1101 100-104 0.0 1101 105-109 0.0 1101 110-114 0.0 1101 115-119 0.0 1101 120-124 0.0 1101 125-129 0.0 1101 130-134 0.0 1101 135-139 0.0 1101 140-144 0.0 1101 145-149 0.0 1101 150 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 1.0 4 1.0 5 0.0 6 1.0 7 3.0 8 1.0 9 4.0 10 6.0 11 7.0 12 2.0 13 11.0 14 9.0 15 9.0 16 10.0 17 12.0 18 19.0 19 19.0 20 20.0 21 22.0 22 26.0 23 31.0 24 44.0 25 48.0 26 71.0 27 87.0 28 92.0 29 114.0 30 154.0 31 164.0 32 213.0 33 326.0 34 421.0 35 684.0 36 880.0 37 486.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 31.002331002331 15.073815073815075 12.017612017612016 41.90624190624191 2 34.1 17.05 20.424999999999997 28.425 3 25.174999999999997 19.825 19.6 35.4 4 29.325000000000003 20.075000000000003 17.974999999999998 32.625 5 31.5 24.125 19.85 24.525 6 28.749999999999996 26.35 19.375 25.525 7 23.525 23.400000000000002 28.525 24.55 8 25.7 22.075 25.05 27.175 9 24.575 20.5 26.650000000000002 28.275 10-14 26.085 24.845 21.705 27.365000000000002 15-19 26.13698904287787 23.355180867563917 22.919897933656877 27.587932155901335 20-24 26.875 23.345 22.325 27.455000000000002 25-29 27.224999999999998 23.23 21.905 27.639999999999997 30-34 26.900000000000002 23.22 22.31 27.57 35-39 26.810000000000002 22.79 22.78 27.62 40-44 26.63 23.135 22.2 28.035 45-49 26.905 22.81 22.95 27.334999999999997 50-54 27.255000000000003 22.615 21.675 28.455000000000002 55-59 27.389999999999997 22.795 21.555 28.26 60-64 27.534999999999997 22.455 21.765 28.244999999999997 65-69 27.08 22.71 22.425 27.785 70-74 27.639999999999997 22.185 22.05 28.125 75-79 27.900000000000002 22.535 22.105 27.46 80-84 27.250000000000004 22.634999999999998 22.655 27.46 85-89 27.229999999999997 21.990000000000002 22.435 28.345 90-94 27.555000000000003 22.09 22.785 27.57 95-99 28.34 22.275 21.759999999999998 27.625 100-104 27.605 22.415 22.189999999999998 27.79 105-109 27.76 22.03 22.25 27.96 110-114 27.905 22.685 21.77 27.639999999999997 115-119 28.065 22.06 22.03 27.845 120-124 28.389999999999997 22.5 22.06 27.05 125-129 28.405 22.035 21.64 27.92 130-134 28.22 21.884999999999998 21.584999999999997 28.310000000000002 135-139 29.220000000000002 21.83 21.72 27.229999999999997 140-144 29.520000000000003 21.78 21.14 27.560000000000002 145-149 30.005 22.035 20.355 27.605 150 30.45 21.925 18.125 29.5 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.5 23 0.5 24 0.5 25 0.5 26 0.5 27 0.5 28 0.5 29 2.0 30 2.5 31 3.0 32 3.5 33 8.0 34 11.5 35 16.5 36 25.0 37 31.5 38 44.0 39 56.0 40 74.5 41 95.5 42 103.0 43 124.5 44 139.0 45 146.5 46 152.5 47 138.0 48 134.0 49 139.0 50 128.5 51 124.5 52 134.5 53 121.5 54 99.5 55 90.0 56 103.0 57 103.5 58 85.5 59 91.5 60 88.0 61 79.0 62 82.5 63 76.5 64 82.5 65 84.5 66 91.0 67 102.5 68 90.0 69 86.0 70 85.0 71 77.5 72 70.0 73 64.5 74 55.5 75 49.0 76 45.5 77 36.0 78 26.5 79 21.0 80 18.5 81 12.0 82 10.0 83 11.0 84 7.5 85 4.0 86 3.5 87 2.0 88 0.5 89 0.5 90 0.5 91 0.5 92 0.0 93 0.0 94 0.5 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 3.4750000000000005 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.065 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 150 4000.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 99.7 #Duplication Level Percentage of deduplicated Percentage of total 1 99.72417251755266 99.425 2 0.25075225677031093 0.5 3 0.025075225677031094 0.075 4 0.0 0.0 5 0.0 0.0 6 0.0 0.0 7 0.0 0.0 8 0.0 0.0 9 0.0 0.0 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.0 0.0 0.0 0.0 0.0 70-71 0.0 0.0 0.0 0.0 0.0 72-73 0.0 0.0 0.0 0.0 0.0 74-75 0.0 0.0 0.0 0.0 0.0 76-77 0.0 0.0 0.0 0.0 0.0 78-79 0.0 0.0 0.0 0.0 0.0 80-81 0.0 0.0 0.0 0.0 0.0 82-83 0.0 0.0 0.0 0.0 0.0 84-85 0.0 0.0 0.0 0.0 0.0 86-87 0.0 0.0 0.0 0.0 0.0 88-89 0.0 0.0 0.0 0.0 0.0 90-91 0.0 0.0 0.0 0.0 0.0 92-93 0.0 0.0 0.0 0.0 0.0 94-95 0.0 0.0 0.0 0.0 0.0 96-97 0.0 0.0 0.0 0.0 0.0 98-99 0.0 0.0 0.0 0.0 0.0 100-101 0.0 0.0 0.0 0.0 0.0 102-103 0.0 0.0 0.0 0.0 0.0 104-105 0.0 0.0 0.0 0.0 0.0 106-107 0.0 0.0 0.0 0.0 0.0 108-109 0.0 0.0 0.0 0.0 0.0 110-111 0.0 0.0 0.0 0.0 0.0 112-113 0.025 0.0 0.0 0.0 0.0 114-115 0.125 0.0 0.0 0.0 0.0 116-117 0.21250000000000002 0.0 0.0 0.0 0.0 118-119 0.375 0.0 0.0 0.0 0.0 120-121 0.475 0.0 0.0 0.0 0.0 122-123 0.525 0.0 0.0 0.0 0.0 124-125 0.6875 0.0 0.0 0.0 0.0 126-127 0.875 0.0 0.0 0.0 0.0 128-129 0.975 0.0 0.0 0.0 0.0 130-131 1.225 0.0 0.0 0.0 0.0 132-133 1.6375 0.0 0.0 0.0 0.0 134-135 1.9 0.0 0.0 0.0 0.0 136-137 2.35 0.0 0.0 0.0 0.0 138 2.55 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content pass >>END_MODULE Read 2239762 spots for SRR6322443.sra Written 2239762 spots for SRR6322443.sra Read 2239762 spots for SRR6322443.sra Written 2239762 spots for SRR6322443.sra Read 2239762 spots for SRR6322443.sra Written 2239762 spots for SRR6322443.sra Read 2239762 spots for SRR6322443.sra Written 2239762 spots for SRR6322443.sra Read 2239762 spots for SRR6322443.sra Written 2239762 spots for SRR6322443.sra Read 2239762 spots for SRR6322443.sra Written 2239762 spots for SRR6322443.sra Read 2239762 spots for SRR6322443.sra Written 2239762 spots for SRR6322443.sra Read 2239762 spots for SRR6322443.sra Written 2239762 spots for SRR6322443.sra Read 2239762 spots for SRR6322443.sra Written 2239762 spots for SRR6322443.sra Read 2239762 spots for SRR6322443.sra Written 2239762 spots for SRR6322443.sra Read 2239762 spots for SRR6322443.sra Written 2239762 spots for SRR6322443.sra Read 2239762 spots for SRR6322443.sra Written 2239762 spots for SRR6322443.sra Read 2239762 spots for SRR6322443.sra Written 2239762 spots for SRR6322443.sra Read 2239762 spots for SRR6322443.sra Written 2239762 spots for SRR6322443.sra Read 2239762 spots for SRR6322443.sra Written 2239762 spots for SRR6322443.sra Read 2239762 spots for SRR6322443.sra Written 2239762 spots for SRR6322443.sra Read 2239762 spots for SRR6322443.sra Written 2239762 spots for SRR6322443.sra Read 2239776 spots for SRR6322443.sra Written 2239776 spots for SRR6322443.sra Read 2239762 spots for SRR6322443.sra Written 2239762 spots for SRR6322443.sra Read 2239762 spots for SRR6322443.sra Written 2239762 spots for SRR6322443.sra SRR ids: ['SRR6322443.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_aa3djztr SRR6322443.sra spots: 44795254 blocks: [[1, 2239762], [2239763, 4479524], [4479525, 6719286], [6719287, 8959048], [8959049, 11198810], [11198811, 13438572], [13438573, 15678334], [15678335, 17918096], [17918097, 20157858], [20157859, 22397620], [22397621, 24637382], [24637383, 26877144], [26877145, 29116906], [29116907, 31356668], [31356669, 33596430], [33596431, 35836192], [35836193, 38075954], [38075955, 40315716], [40315717, 42555478], [42555479, 44795254]] SRR6322443 file size 16482679 SRR6322443 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR6322443 SRR6322443_1.fastq Input file: SRR6322443_1.fastq trimmed: SRR6322443-trimmed.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- minimum overlap length for adapter detection (-k): inf -- number of concurrent threads (-t): 20 Sat Dec 7 11:01:26 2024 >> started Sat Dec 7 11:01:54 2024 >> done (28.579s) 44795254 reads processed; of these: 48205 ( 0.11%) short reads filtered out after trimming by size control 34857 ( 0.08%) empty reads filtered out after trimming by size control 44712192 (99.81%) reads available; of these: 25269247 (56.52%) trimmed reads available after processing 19442945 (43.48%) untrimmed reads available after processing Length distribution of reads after trimming: length count percentage 18 5852 0.01% 19 6149 0.01% 20 6621 0.01% 21 7152 0.02% 22 7243 0.02% 23 8045 0.02% 24 8502 0.02% 25 9402 0.02% 26 10788 0.02% 27 10081 0.02% 28 10867 0.02% 29 11353 0.03% 30 11141 0.02% 31 11319 0.03% 32 11798 0.03% 33 11869 0.03% 34 12199 0.03% 35 12520 0.03% 36 13001 0.03% 37 13336 0.03% 38 13741 0.03% 39 14495 0.03% 40 14548 0.03% 41 15040 0.03% 42 15717 0.04% 43 16068 0.04% 44 16460 0.04% 45 16377 0.04% 46 17366 0.04% 47 18259 0.04% 48 18100 0.04% 49 18038 0.04% 50 18125 0.04% 51 15714 0.04% 52 15303 0.03% 53 16562 0.04% 54 16581 0.04% 55 16796 0.04% 56 17016 0.04% 57 17635 0.04% 58 16828 0.04% 59 17240 0.04% 60 17954 0.04% 61 18616 0.04% 62 19103 0.04% 63 19631 0.04% 64 21539 0.05% 65 25550 0.06% 66 25204 0.06% 67 24696 0.06% 68 25155 0.06% 69 24349 0.05% 70 24742 0.06% 71 26782 0.06% 72 28167 0.06% 73 28808 0.06% 74 29798 0.07% 75 29871 0.07% 76 27084 0.06% 77 28193 0.06% 78 31121 0.07% 79 32498 0.07% 80 34509 0.08% 81 37433 0.08% 82 38535 0.09% 83 40283 0.09% 84 41792 0.09% 85 43438 0.10% 86 45367 0.10% 87 46994 0.11% 88 48504 0.11% 89 51065 0.11% 90 53817 0.12% 91 55769 0.12% 92 58222 0.13% 93 60587 0.14% 94 62179 0.14% 95 65960 0.15% 96 69003 0.15% 97 72605 0.16% 98 76574 0.17% 99 80048 0.18% 100 83759 0.19% 101 89088 0.20% 102 93296 0.21% 103 96752 0.22% 104 102978 0.23% 105 108044 0.24% 106 111448 0.25% 107 118467 0.26% 108 122757 0.27% 109 129239 0.29% 110 137914 0.31% 111 145187 0.32% 112 153875 0.34% 113 164057 0.37% 114 177226 0.40% 115 191952 0.43% 116 209567 0.47% 117 214631 0.48% 118 212037 0.47% 119 215614 0.48% 120 217594 0.49% 121 226379 0.51% 122 237161 0.53% 123 244192 0.55% 124 254705 0.57% 125 265964 0.59% 126 278643 0.62% 127 289868 0.65% 128 306583 0.69% 129 320856 0.72% 130 335423 0.75% 131 357252 0.80% 132 372377 0.83% 133 395443 0.88% 134 416297 0.93% 135 424840 0.95% 136 442231 0.99% 137 474431 1.06% 138 505150 1.13% 139 541383 1.21% 140 592994 1.33% 141 642850 1.44% 142 712728 1.59% 143 808648 1.81% 144 917540 2.05% 145 1066587 2.39% 146 1283019 2.87% 147 1507224 3.37% 148 1881865 4.21% 149 3918340 8.76% 150 19442945 43.48% 44712192 reads passed initial QC criterion=sequence-density sequence-density=1.30 sequence-density-rank=1 fanout-score=73.46 fanout-score-rank=11 prefix-density=2.01 prefix-fanout=47.5 sequence=AGATCGGAAGAGC criterion=fanout-score sequence-density=0.02 sequence-density-rank=28 fanout-score=1729.49 fanout-score-rank=1 prefix-density=1.13 prefix-fanout=29.1 sequence=GCGGCGGCGAACCGCCCCCGTCGCCGCGATCCGAACACTTCACCGGACCATTCAATCGGTAGGAGCGACGGGCGGTGTGTACAAAGGGCAGGGACGTAGTCAACGCGAGCTGATGACTCGCGCTTACTAGGCATTCCTCGTTGAAGACCAACAATTGCAATGATCTATCCCCATCACGATGAAATTTCCCAAGATTACCCGGGCCTGTCGGCCAAGGCTATATACTCGTTGAATACATCAGTGTAGCGCGCGTGCGGCCCAGAACATCTAAGGGCATCACAGACCTGTTATTGCCTCAAACTTCCGTCGCCTAAACGGCGATAGTCCCTCTAAGAAGCTAGCTGCGGAGGGATGGCTCCGCATAGCTAGTTAGCAGGCTGAGGTCTCGTTCGTTAACGGAATTAACCAGACAAATCGCTCCACCAACTAAGAACGGCCATGCACCACCACCCATAGAATCAAGAAAGAGCTCTCAGTCTGTCAATCCTTGCTATGTCTGGACCTGGTAAGT Started job on | Dec 07 11:02:15 Started mapping on | Dec 07 11:02:15 Finished on | Dec 07 11:03:25 Mapping speed, Million of reads per hour | 2299.48 Number of input reads | 44712192 Average input read length | 139 UNIQUE READS: Uniquely mapped reads number | 42359279 Uniquely mapped reads % | 94.74% Average mapped length | 139.00 Number of splices: Total | 16786419 Number of splices: Annotated (sjdb) | 15870155 Number of splices: GT/AG | 16549246 Number of splices: GC/AG | 190718 Number of splices: AT/AC | 10438 Number of splices: Non-canonical | 36017 Mismatch rate per base, % | 0.27% Deletion rate per base | 0.02% Deletion average length | 2.51 Insertion rate per base | 0.01% Insertion average length | 2.27 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 775566 % of reads mapped to multiple loci | 1.73% Number of reads mapped to too many loci | 1177689 % of reads mapped to too many loci | 2.63% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 0.59% % of reads unmapped: other | 0.30% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 1577347 1577347 1577347 N_multimapping 775566 775566 775566 N_noFeature 974659 21423596 21337980 N_ambiguous 652221 42450 45269 UnstrandedReadsAssigned:40732399 PositiveStrandReadsAssigned:20893233 NegativeStrandReadsAssigned:20976030 Dataset is classified unstranded MeadianReadLen=150 20thPercentileLength=138 echo kmer=133 SRR6322443 Starting Kallisto single end mapping to ensembl reference transcriptome. kmer=31 [quant] fragment length distribution is truncated gaussian with mean = 100, sd = 20 [index] k-mer length: 31 [index] number of targets: 52,972 [index] number of k-mers: 66,720,672 [index] number of equivalence classes: 111,837 [quant] running in single-end mode [quant] will process file 1: SRR6322443-trimmed.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 44,712,192 reads, 41,922,520 reads pseudoaligned [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,294 rounds 52973 SRR6322443.ke.tsv 35125 SRR6322443.se.tsv 88098 total ==> SRR6322443.ke.tsv <== target_id length eff_length est_counts tpm PNS24245 936 837 7.69323 0.352981 PNS24247 1044 945 50.9097 2.06889 PNS24249 1928 1829 881.694 18.5128 PNS24246 1044 945 50.9097 2.06889 PNS24248 1044 945 50.9097 2.06889 PNS24244 1471 1372 82.8832 2.31996 PNS24243 293 194 12 2.37546 KQK14069 1603 1504 12547.5 320.39 KQK14071 474 375 2290.55 234.572 ==> SRR6322443.se.tsv <== BRADI_1g14170v3 15101 BRADI_1g53295v3 404 BRADI_1g59795v3 402 BRADI_1g07683v3 0 BRADI_1g00485v3 50 BRADI_1g20270v3 1009 BRADI_1g74790v3 977 BRADI_1g09890v3 0 BRADI_1g77505v3 364 BRADI_1g48960v3 0 SRR6322443 completed mapping pipeline successfully