Starting /dee2/code/volunteer_pipeline.sh SRR6322446 current disk space = 1543157993472 free memory = 1606489552 SRR6322446 SRAfilesize 049ca6e425208b31d42e103e40548c98 SRR6322446.sra SRR6322446.sra file validated SRR6322446 is single end SRR6322446 is conventional basespace SRR6322446 read1 length is 50 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR6322446_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 50 %GC 53 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.49875 32.0 32.0 32.0 32.0 32.0 2 30.88375 32.0 32.0 32.0 32.0 32.0 3 32.95625 37.0 32.0 37.0 27.0 37.0 4 36.11625 37.0 37.0 37.0 32.0 37.0 5 36.05875 37.0 37.0 37.0 37.0 37.0 6 40.2065 41.0 41.0 41.0 37.0 41.0 7 38.36725 41.0 37.0 41.0 32.0 41.0 8 40.1555 41.0 41.0 41.0 37.0 41.0 9 38.83325 41.0 41.0 41.0 32.0 41.0 10 40.3215 41.0 41.0 41.0 37.0 41.0 11 40.51925 41.0 41.0 41.0 41.0 41.0 12 40.34425 41.0 41.0 41.0 41.0 41.0 13 39.91375 41.0 41.0 41.0 37.0 41.0 14 38.6905 41.0 41.0 41.0 32.0 41.0 15 39.818 41.0 41.0 41.0 37.0 41.0 16 39.69475 41.0 41.0 41.0 37.0 41.0 17 40.36525 41.0 41.0 41.0 41.0 41.0 18 39.955 41.0 41.0 41.0 37.0 41.0 19 40.371 41.0 41.0 41.0 41.0 41.0 20 40.13525 41.0 41.0 41.0 37.0 41.0 21 40.187 41.0 41.0 41.0 41.0 41.0 22 40.37125 41.0 41.0 41.0 41.0 41.0 23 39.92575 41.0 41.0 41.0 37.0 41.0 24 37.82925 41.0 37.0 41.0 27.0 41.0 25 38.18775 41.0 37.0 41.0 32.0 41.0 26 37.866 41.0 37.0 41.0 27.0 41.0 27 39.56225 41.0 41.0 41.0 37.0 41.0 28 38.07625 41.0 37.0 41.0 32.0 41.0 29 38.24375 41.0 37.0 41.0 32.0 41.0 30 38.73425 41.0 41.0 41.0 32.0 41.0 31 35.147 41.0 32.0 41.0 12.0 41.0 32 35.372 41.0 32.0 41.0 22.0 41.0 33 36.326 41.0 37.0 41.0 22.0 41.0 34 32.16925 37.0 27.0 41.0 12.0 41.0 35 38.407 41.0 37.0 41.0 32.0 41.0 36 39.2325 41.0 41.0 41.0 37.0 41.0 37 34.54475 41.0 32.0 41.0 12.0 41.0 38 38.72125 41.0 37.0 41.0 32.0 41.0 39 39.7905 41.0 41.0 41.0 37.0 41.0 40 39.32725 41.0 41.0 41.0 37.0 41.0 41 39.25625 41.0 41.0 41.0 37.0 41.0 42 39.697 41.0 41.0 41.0 37.0 41.0 43 39.46725 41.0 41.0 41.0 37.0 41.0 44 40.00325 41.0 41.0 41.0 37.0 41.0 45 39.64875 41.0 41.0 41.0 37.0 41.0 46 38.99225 41.0 41.0 41.0 37.0 41.0 47 38.1795 41.0 37.0 41.0 32.0 41.0 48 39.512 41.0 41.0 41.0 37.0 41.0 49 39.73225 41.0 41.0 41.0 37.0 41.0 50 38.61475 41.0 41.0 41.0 32.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 17 1.0 18 0.0 19 0.0 20 0.0 21 1.0 22 0.0 23 0.0 24 2.0 25 2.0 26 9.0 27 7.0 28 16.0 29 24.0 30 28.0 31 52.0 32 61.0 33 79.0 34 125.0 35 173.0 36 241.0 37 381.0 38 623.0 39 988.0 40 1187.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.2353088272068 8.202050512628158 5.026256564141035 45.53638409602401 2 22.54526906401428 8.977301708747769 42.54016832440704 25.93726090283091 3 23.375 13.175 26.025 37.425000000000004 4 29.625 16.975 23.474999999999998 29.925 5 30.625000000000004 21.825 27.0 20.549999999999997 6 25.624999999999996 25.4 25.5 23.474999999999998 7 21.475 22.825 38.875 16.825000000000003 8 20.95 19.55 34.8 24.7 9 21.775 18.725 35.65 23.849999999999998 10 25.2 27.425 27.900000000000002 19.475 11 29.075 20.8 24.6 25.525 12 26.174999999999997 18.975 27.625 27.224999999999998 13 25.55 23.9 25.174999999999997 25.374999999999996 14 26.05 21.5 26.075 26.375 15 25.4 22.725 26.200000000000003 25.674999999999997 16 26.174999999999997 21.175 25.650000000000002 27.0 17 27.425 20.8 25.324999999999996 26.450000000000003 18 25.4 22.325 25.374999999999996 26.900000000000002 19 27.725 22.125 23.625 26.525 20 27.650000000000002 21.15 25.525 25.674999999999997 21 25.924999999999997 22.15 25.124999999999996 26.8 22 27.55 21.475 24.099999999999998 26.875 23 26.6 22.275 25.2 25.924999999999997 24 26.075 19.875 25.025 29.025000000000002 25 26.424999999999997 21.825 24.875 26.875 26 25.95 23.35 24.875 25.825 27 25.174999999999997 23.3 26.0 25.525 28 26.900000000000002 22.675 24.975 25.45 29 25.224999999999998 22.075 26.8 25.900000000000002 30 23.325000000000003 22.375 27.625 26.674999999999997 31 27.025 21.725 23.0 28.249999999999996 32 25.374999999999996 21.349999999999998 27.925 25.35 33 25.55 19.85 25.45 29.15 34 27.400000000000002 20.200000000000003 24.349999999999998 28.050000000000004 35 23.375 23.3 25.474999999999998 27.85 36 25.775 20.7 25.05 28.475 37 28.775000000000002 20.65 25.275 25.3 38 27.200000000000003 21.825 24.275 26.700000000000003 39 26.950000000000003 21.099999999999998 25.124999999999996 26.825 40 25.2 22.85 25.575 26.375 41 25.174999999999997 20.65 25.974999999999998 28.199999999999996 42 26.85 20.225 26.075 26.85 43 26.150000000000002 20.5 25.15 28.199999999999996 44 25.424999999999997 21.099999999999998 25.924999999999997 27.55 45 27.075 20.8 25.674999999999997 26.450000000000003 46 26.375 20.3 26.3 27.025 47 24.8 22.675 25.474999999999998 27.05 48 25.324999999999996 20.424999999999997 26.525 27.725 49 26.25 22.25 24.375 27.125 50 26.700000000000003 22.275 23.599999999999998 27.425 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.5 2 1.0 3 0.5 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 1.0 22 2.0 23 2.5 24 3.0 25 4.5 26 6.0 27 8.5 28 11.0 29 13.0 30 15.0 31 21.0 32 27.0 33 41.0 34 55.0 35 73.0 36 91.0 37 107.0 38 123.0 39 138.5 40 154.0 41 206.0 42 258.0 43 272.5 44 287.0 45 271.0 46 255.0 47 298.0 48 341.0 49 304.0 50 267.0 51 261.5 52 256.0 53 245.5 54 235.0 55 231.0 56 227.0 57 213.5 58 200.0 59 202.5 60 205.0 61 190.0 62 175.0 63 166.0 64 157.0 65 145.5 66 134.0 67 141.5 68 149.0 69 135.0 70 121.0 71 105.0 72 89.0 73 74.0 74 59.0 75 51.0 76 43.0 77 33.0 78 23.0 79 21.5 80 20.0 81 13.0 82 6.0 83 5.0 84 4.0 85 2.5 86 1.0 87 0.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.025 2 1.975 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 50 4000.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 82.025 #Duplication Level Percentage of deduplicated Percentage of total 1 88.1438585797013 72.3 2 7.497714111551357 12.3 3 2.3773239865894547 5.8500000000000005 4 0.9448338921060652 3.1 5 0.3962206644315757 1.625 6 0.1828710758914965 0.8999999999999999 7 0.15239256324291375 0.8750000000000001 8 0.12191405059433098 0.8 9 0.030478512648582746 0.22499999999999998 >10 0.15239256324291375 2.025 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATGATTAACAGCTGCCCTCGACGAAGAAGTCCATTGAACACATTGCGG 25 0.625 No Hit GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC 25 0.625 TruSeq Adapter, Index 5 (100% over 50bp) GGGAAATTCTGACCGTTGAGGCGTGCGATACTGCCAGCACGTGGGTTGTA 11 0.27499999999999997 No Hit CGGGAAATTCTGACCGTTGAGGCGTGCGATACTGCCAGCACGTGGGTTGT 10 0.25 No Hit CTTTATTTTTGTCACTGCTGCTACATCGACTAAAACAGCGGTGATCCCTA 10 0.25 No Hit CTCCTGTGCCTTAGGGGTCGAAGATGAATCCACGTCTCCGATCGGGTAAC 9 0.22499999999999998 No Hit CTCCGATCGGGTAACTCTGGCAGCTGCTTTGGGGGAAATTCGGAGTCAAC 8 0.2 No Hit CTCATCAAAATACTCCGTCAGACCAGCTTGTGACCTCACTTGCGTAAGTG 8 0.2 No Hit CTCTCATCTTGTTGTTGATGTGGGCACGGCTGCTCCGGCTGAACCGACCA 8 0.2 No Hit GGTATGATTAACAGCTGCCCTCGACGAAGAAGTCCATTGAACACATTGCG 8 0.2 No Hit CTTTATTTCACCCTAATAGTTATCATACTAACTCATTCACTCCTGTGCCT 7 0.17500000000000002 No Hit CTGGTATCCTTCACTCTCCGCCTTCTTTAAAACGACATAGTTTTGTGGTA 7 0.17500000000000002 No Hit CTCTGGGCACCCAGGAAAAGCCAGTCCTACAAAACCACTACCTTCGAGAA 7 0.17500000000000002 No Hit CTTGAAAGCGATATACTGGTATCCTTCACTCTCCGCCTTCTTTAAAACGA 7 0.17500000000000002 No Hit CTCCATGTCGCCGCCGCGGCCGCCGCGGCGCCTCTCTCCCTCCTCGCCCT 7 0.17500000000000002 No Hit CTGCGATGTGACTAACCATGGAGTTTGCATTGGTCTTAAATGCAATGTGT 6 0.15 No Hit GGGGGTGTTGTGGTATCGAGGTAATAAAAGGCCTCGAGGCTCGATAACAC 6 0.15 No Hit GCCTTCTTTAAAACGACATAGTTTTGTGGTATGATTAACAGCTGCCCTCG 6 0.15 No Hit GTTTGCGTTGGTCTTGAAAGCGATATACTGGTATCCTTCACTCTCCGCCT 6 0.15 No Hit CTCCTTGTGTAAACTGGTGGACTCTCTGGTGCACATCTCCCAACTTTTGG 6 0.15 No Hit CCTAATAGTTATCATACTAACTCATTCACTCCTGTGCCTTAGGGGTCGAA 6 0.15 No Hit CCTGGCACCTGTTCTGCTGCACTGTAGGCCACGGTCCTAGGATTGACGTC 5 0.125 No Hit GTGGTATCGAGGTAATAAAAGGCCTCGAGGCTCGATAACACGACGTATGA 5 0.125 No Hit CTCGATTGTCCTTCCTGAGGTTCGATTGGTAGGAAGGGTTGTTTCTGCTG 5 0.125 No Hit CGTTGGTCTTGAAAGCGATATACTGGTATCCTTCACTCTCCGCCTTCTTT 5 0.125 No Hit CTCTGGATGGGTTTGCTCAGGCTTGACACGAGCGAATCGGCACGGAAATA 5 0.125 No Hit GGGCATTAATGTTCCAGAATGGTGAAACAATCGCATTCTTCTGGAGATTT 5 0.125 No Hit CTGGAAGCTCCGTGGCCCGAACACGTACGGCCGCCGGCTCTCCTGCTGCT 5 0.125 No Hit CGTCATTCTGATTCTGAATTATTCGTGATGTTTGTTCATTGATACCAAAG 5 0.125 No Hit CTGGAGATTTACCCTCGTAGCACTCATTTGCACGAGGTTAAGGATCGGGA 5 0.125 No Hit CTCAACACAAGTACACACAGTTCCCGTCACGCACCCAGGCCCGCCGCTCC 5 0.125 No Hit GTCCGCACTCCTGTGACTGGGCCCCGAACCGGCGCGAAGGGATCTGACCT 5 0.125 No Hit CTGCTGTTGTTGGGTGACGACGGGCGTCAGAAATTGAAGTCCATATTCCA 5 0.125 No Hit CTCCTTGTGTAAACTGGTGTACTCTCTGGTGCACATCTCCCAACTTTTGG 5 0.125 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 14 0.0 0.0 0.0 0.0 0.0 15 0.0 0.0 0.0 0.0 0.0 16 0.0 0.0 0.0 0.0 0.0 17 0.0 0.0 0.0 0.0 0.0 18 0.0 0.0 0.0 0.0 0.0 19 0.0 0.0 0.0 0.0 0.0 20 0.0 0.0 0.0 0.0 0.0 21 0.0 0.0 0.0 0.0 0.0 22 0.0 0.0 0.0 0.0 0.0 23 0.0 0.0 0.0 0.0 0.0 24 0.0 0.0 0.0 0.0 0.0 25 0.0 0.0 0.0 0.0 0.0 26 0.0 0.0 0.0 0.0 0.0 27 0.0 0.0 0.0 0.0 0.0 28 0.0 0.0 0.0 0.0 0.0 29 0.0 0.0 0.0 0.0 0.0 30 0.0 0.0 0.0 0.0 0.0 31 0.0 0.0 0.0 0.0 0.0 32 0.0 0.0 0.0 0.0 0.0 33 0.0 0.0 0.0 0.0 0.0 34 0.0 0.0 0.0 0.0 0.0 35 0.0 0.0 0.0 0.0 0.0 36 0.0 0.0 0.0 0.0 0.0 37 0.0 0.0 0.0 0.0 0.0 38 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content pass >>END_MODULE Rejected 1476815 READS because READLEN < 1 Read 1476815 spots for SRR6322446.sra Written 1476815 spots for SRR6322446.sra Rejected 1476815 READS because READLEN < 1 Read 1476815 spots for SRR6322446.sra Written 1476815 spots for SRR6322446.sra Rejected 1476815 READS because READLEN < 1 Read 1476815 spots for SRR6322446.sra Written 1476815 spots for SRR6322446.sra Rejected 1476815 READS because READLEN < 1 Read 1476815 spots for SRR6322446.sra Written 1476815 spots for SRR6322446.sra Rejected 1476815 READS because READLEN < 1 Read 1476815 spots for SRR6322446.sra Written 1476815 spots for SRR6322446.sra Rejected 1476815 READS because READLEN < 1 Read 1476815 spots for SRR6322446.sra Written 1476815 spots for SRR6322446.sra Rejected 1476815 READS because READLEN < 1 Read 1476815 spots for SRR6322446.sra Written 1476815 spots for SRR6322446.sra Rejected 1476815 READS because READLEN < 1 Read 1476815 spots for SRR6322446.sra Written 1476815 spots for SRR6322446.sra Rejected 1476815 READS because READLEN < 1 Read 1476815 spots for SRR6322446.sra Written 1476815 spots for SRR6322446.sra Rejected 1476815 READS because READLEN < 1 Read 1476815 spots for SRR6322446.sra Written 1476815 spots for SRR6322446.sra Rejected 1476815 READS because READLEN < 1 Read 1476815 spots for SRR6322446.sra Written 1476815 spots for SRR6322446.sra Rejected 1476822 READS because READLEN < 1 Read 1476822 spots for SRR6322446.sra Written 1476822 spots for SRR6322446.sra Rejected 1476815 READS because READLEN < 1 Read 1476815 spots for SRR6322446.sra Written 1476815 spots for SRR6322446.sra Rejected 1476815 READS because READLEN < 1 Read 1476815 spots for SRR6322446.sra Written 1476815 spots for SRR6322446.sra Rejected 1476815 READS because READLEN < 1 Read 1476815 spots for SRR6322446.sra Written 1476815 spots for SRR6322446.sra Rejected 1476815 READS because READLEN < 1 Read 1476815 spots for SRR6322446.sra Written 1476815 spots for SRR6322446.sra Rejected 1476815 READS because READLEN < 1 Read 1476815 spots for SRR6322446.sra Written 1476815 spots for SRR6322446.sra Rejected 1476815 READS because READLEN < 1 Read 1476815 spots for SRR6322446.sra Written 1476815 spots for SRR6322446.sra Rejected 1476815 READS because READLEN < 1 Read 1476815 spots for SRR6322446.sra Written 1476815 spots for SRR6322446.sra Rejected 1476815 READS because READLEN < 1 Read 1476815 spots for SRR6322446.sra Written 1476815 spots for SRR6322446.sra SRR ids: ['SRR6322446.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_gmbed7ou SRR6322446.sra spots: 29536307 blocks: [[1, 1476815], [1476816, 2953630], [2953631, 4430445], [4430446, 5907260], [5907261, 7384075], [7384076, 8860890], [8860891, 10337705], [10337706, 11814520], [11814521, 13291335], [13291336, 14768150], [14768151, 16244965], [16244966, 17721780], [17721781, 19198595], [19198596, 20675410], [20675411, 22152225], [22152226, 23629040], [23629041, 25105855], [25105856, 26582670], [26582671, 28059485], [28059486, 29536307]] SRR6322446 file size 4131842 SRR6322446 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR6322446 SRR6322446_1.fastq Input file: SRR6322446_1.fastq trimmed: SRR6322446-trimmed.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- minimum overlap length for adapter detection (-k): inf -- number of concurrent threads (-t): 20 Sat Dec 7 12:27:47 2024 >> started Sat Dec 7 12:28:08 2024 >> done (20.796s) 29536307 reads processed; of these: 156 ( 0.00%) short reads filtered out after trimming by size control 276648 ( 0.94%) empty reads filtered out after trimming by size control 29259503 (99.06%) reads available; of these: 24 ( 0.00%) trimmed reads available after processing 29259479 (100.00%) untrimmed reads available after processing Length distribution of reads after trimming: length count percentage 18 24 0.00% 19 0 0.00% 20 0 0.00% 21 0 0.00% 22 0 0.00% 23 0 0.00% 24 0 0.00% 25 0 0.00% 26 0 0.00% 27 0 0.00% 28 0 0.00% 29 0 0.00% 30 0 0.00% 31 0 0.00% 32 0 0.00% 33 0 0.00% 34 0 0.00% 35 0 0.00% 36 0 0.00% 37 0 0.00% 38 0 0.00% 39 0 0.00% 40 0 0.00% 41 0 0.00% 42 0 0.00% 43 0 0.00% 44 0 0.00% 45 0 0.00% 46 0 0.00% 47 0 0.00% 48 0 0.00% 49 0 0.00% 50 29259479 100.00% 29259503 reads passed initial QC criterion=sequence-density sequence-density=1.25 sequence-density-rank=1 fanout-score=2.05 fanout-score-rank=29 prefix-density=1.29 prefix-fanout=2.0 sequence=TTTGGCTTTGGC criterion=fanout-score sequence-density=0.02 sequence-density-rank=33 fanout-score=58.24 fanout-score-rank=1 prefix-density=0.34 prefix-fanout=3.0 sequence=TTTATTTATATTTTATTGAGAACACAAACATCAAGTGCTGCACACTTGCGATTTATTTATTTTACTCTACAGTTCTCATATATATTAGTTCATTCGCTCTGATGCCTTGCGACTTGAAGCCGATTCCTCAAAACTCTGGTAGCTCAATGGAGGGAATTTAGTAGTGAAGGCGCCAAACTCTTCTCCCC Started job on | Dec 07 12:28:24 Started mapping on | Dec 07 12:28:25 Finished on | Dec 07 12:28:49 Mapping speed, Million of reads per hour | 4388.93 Number of input reads | 29259503 Average input read length | 49 UNIQUE READS: Uniquely mapped reads number | 22377565 Uniquely mapped reads % | 76.48% Average mapped length | 49.81 Number of splices: Total | 1993732 Number of splices: Annotated (sjdb) | 1890851 Number of splices: GT/AG | 1933625 Number of splices: GC/AG | 22164 Number of splices: AT/AC | 1021 Number of splices: Non-canonical | 36922 Mismatch rate per base, % | 0.33% Deletion rate per base | 0.00% Deletion average length | 1.58 Insertion rate per base | 0.00% Insertion average length | 1.50 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 6129232 % of reads mapped to multiple loci | 20.95% Number of reads mapped to too many loci | 625957 % of reads mapped to too many loci | 2.14% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 0.40% % of reads unmapped: other | 0.03% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 752706 752706 752706 N_multimapping 6129232 6129232 6129232 N_noFeature 818289 22103675 936232 N_ambiguous 193404 661 37664 UnstrandedReadsAssigned:21365872 PositiveStrandReadsAssigned:273229 NegativeStrandReadsAssigned:21403669 Dataset is classified negative stranded MeadianReadLen=50 20thPercentileLength=50 echo kmer=45 SRR6322446 Starting Kallisto single end mapping to ensembl reference transcriptome. kmer=31 [quant] fragment length distribution is truncated gaussian with mean = 100, sd = 20 [index] k-mer length: 31 [index] number of targets: 52,972 [index] number of k-mers: 66,720,672 [index] number of equivalence classes: 111,837 [quant] running in single-end mode [quant] will process file 1: SRR6322446-trimmed.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 29,259,503 reads, 26,347,607 reads pseudoaligned [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,203 rounds 52973 SRR6322446.ke.tsv 35125 SRR6322446.se.tsv 88098 total ==> SRR6322446.ke.tsv <== target_id length eff_length est_counts tpm PNS24245 936 837 0 0 PNS24247 1044 945 100.904 5.55763 PNS24249 1928 1829 261.908 7.45327 PNS24246 1044 945 100.904 5.55763 PNS24248 1044 945 100.904 5.55763 PNS24244 1471 1372 22.3783 0.848954 PNS24243 293 194 0 0 KQK14069 1603 1504 1058.27 36.6236 KQK14071 474 375 357.486 49.6179 ==> SRR6322446.se.tsv <== BRADI_1g14170v3 1801 BRADI_1g53295v3 714 BRADI_1g59795v3 217 BRADI_1g07683v3 0 BRADI_1g00485v3 3 BRADI_1g20270v3 41 BRADI_1g74790v3 1 BRADI_1g09890v3 0 BRADI_1g77505v3 177 BRADI_1g48960v3 3 SRR6322446 completed mapping pipeline successfully