Starting /dee2/code/volunteer_pipeline.sh SRR6322447
    current disk space = 1543381622784
    free memory = 1601920860 
SRR6322447 SRAfilesize
870f5e4d007b33818f6dd8f17823de28  SRR6322447.sra
SRR6322447.sra file validated
SRR6322447 is single end
SRR6322447 is conventional basespace
SRR6322447 read1 length is 150 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR6322447_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	150
%GC	55
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.7235	34.0	33.0	34.0	31.0	34.0
2	32.599	34.0	34.0	34.0	31.0	34.0
3	33.124	34.0	34.0	34.0	31.0	34.0
4	36.59275	37.0	37.0	37.0	35.0	37.0
5	36.6605	37.0	37.0	37.0	35.0	37.0
6	36.66025	37.0	37.0	37.0	36.0	37.0
7	36.63125	37.0	37.0	37.0	35.0	37.0
8	36.66475	37.0	37.0	37.0	36.0	37.0
9	38.6085	39.0	39.0	39.0	38.0	39.0
10-14	38.865300000000005	39.4	39.2	39.4	38.0	39.4
15-19	39.99275	41.0	40.0	41.0	38.2	41.0
20-24	39.88895	41.0	40.0	41.0	38.0	41.0
25-29	39.49265	41.0	39.8	41.0	37.0	41.0
30-34	39.135400000000004	40.8	38.8	41.0	35.4	41.0
35-39	38.9246	41.0	38.6	41.0	35.0	41.0
40-44	38.3754	40.0	37.0	41.0	35.0	41.0
45-49	37.7405	40.0	35.0	41.0	33.6	41.0
50-54	36.88125	38.8	35.0	40.8	32.8	41.0
55-59	36.3868	37.2	35.0	41.0	32.2	41.0
60-64	35.94085	35.8	35.0	39.8	32.4	41.0
65-69	35.2932	35.0	35.0	38.8	31.8	41.0
70-74	34.66375000000001	35.0	35.0	36.8	31.8	39.4
75-79	33.7152	35.0	34.2	35.8	30.2	37.4
80-84	33.2591	35.0	34.0	35.0	30.0	36.4
85-89	32.88100000000001	35.0	34.0	35.0	29.2	35.6
90-94	32.53150000000001	35.0	33.4	35.0	28.6	35.0
95-99	32.16695	35.0	33.0	35.0	27.4	35.0
100-104	31.670250000000003	35.0	33.0	35.0	24.8	35.0
105-109	31.3405	35.0	32.8	35.0	24.0	35.0
110-114	30.585500000000003	34.8	31.4	35.0	20.0	35.0
115-119	30.01495	34.0	30.6	35.0	17.8	35.0
120-124	29.318399999999997	34.0	29.4	35.0	9.2	35.0
125-129	28.8091	34.0	29.0	35.0	3.6	35.0
130-134	28.045500000000004	33.8	27.4	35.0	2.0	35.0
135-139	26.953200000000002	33.0	25.0	35.0	2.0	35.0
140-144	26.0265	32.4	23.4	35.0	2.0	35.0
145-149	24.18315	31.8	8.6	34.2	2.0	35.0
150	17.4565	20.0	2.0	30.0	2.0	33.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.0
1101	2	0.0
1101	3	0.0
1101	4	0.0
1101	5	0.0
1101	6	0.0
1101	7	0.0
1101	8	0.0
1101	9	0.0
1101	10-14	0.0
1101	15-19	0.0
1101	20-24	0.0
1101	25-29	0.0
1101	30-34	0.0
1101	35-39	0.0
1101	40-44	0.0
1101	45-49	0.0
1101	50-54	0.0
1101	55-59	0.0
1101	60-64	0.0
1101	65-69	0.0
1101	70-74	0.0
1101	75-79	0.0
1101	80-84	0.0
1101	85-89	0.0
1101	90-94	0.0
1101	95-99	0.0
1101	100-104	0.0
1101	105-109	0.0
1101	110-114	0.0
1101	115-119	0.0
1101	120-124	0.0
1101	125-129	0.0
1101	130-134	0.0
1101	135-139	0.0
1101	140-144	0.0
1101	145-149	0.0
1101	150	0.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	3.0
3	2.0
4	3.0
5	4.0
6	2.0
7	4.0
8	5.0
9	1.0
10	6.0
11	5.0
12	7.0
13	8.0
14	14.0
15	9.0
16	11.0
17	14.0
18	12.0
19	18.0
20	22.0
21	26.0
22	33.0
23	34.0
24	38.0
25	45.0
26	41.0
27	56.0
28	66.0
29	94.0
30	122.0
31	124.0
32	196.0
33	247.0
34	405.0
35	660.0
36	982.0
37	677.0
38	4.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	31.105263157894736	14.973684210526317	12.105263157894736	41.81578947368421
2	33.025	16.225	21.375	29.375
3	24.725	19.925	17.825	37.525
4	29.2	20.349999999999998	17.974999999999998	32.475
5	31.35	23.625	20.1	24.925
6	28.975	27.575	17.9	25.55
7	22.75	23.225	29.549999999999997	24.474999999999998
8	25.924999999999997	21.975	24.45	27.650000000000002
9	24.975	19.375	26.1	29.549999999999997
10-14	27.18	24.529999999999998	21.955	26.334999999999997
15-19	26.779999999999998	23.125	22.31	27.785
20-24	26.91	23.5	21.82	27.77
25-29	26.955000000000002	23.145	22.125	27.775
30-34	27.139999999999997	23.369999999999997	21.965	27.525
35-39	27.13	23.11	21.985	27.775
40-44	27.310000000000002	22.97	22.02	27.700000000000003
45-49	27.025	23.225	22.35	27.400000000000002
50-54	27.455000000000002	22.935	22.205	27.405
55-59	27.715	22.564999999999998	21.834999999999997	27.884999999999998
60-64	27.925	22.78	22.255	27.04
65-69	27.255000000000003	22.89	22.325	27.529999999999998
70-74	27.465	22.055	22.86	27.62
75-79	27.395000000000003	22.415	22.11	28.08
80-84	27.589999999999996	22.400000000000002	22.314999999999998	27.694999999999997
85-89	28.04	22.515	21.27	28.175
90-94	26.965	22.040000000000003	22.48	28.515
95-99	27.975	22.36	22.41	27.255000000000003
100-104	27.839999999999996	22.435	22.12	27.605
105-109	27.455000000000002	22.040000000000003	22.689999999999998	27.815
110-114	28.34	21.98	22.49	27.189999999999998
115-119	28.425	21.66	22.040000000000003	27.875
120-124	27.735	22.085	22.075	28.105000000000004
125-129	28.155	22.035	22.220000000000002	27.589999999999996
130-134	28.299999999999997	22.57	21.695	27.435
135-139	28.42	21.89	21.995	27.694999999999997
140-144	28.754999999999995	22.3	21.315	27.63
145-149	28.92	21.9	21.5	27.68
150	31.45	21.475	17.825	29.25
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1.0
1	1.0
2	0.5
3	0.0
4	0.0
5	0.5
6	0.5
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.5
27	0.5
28	0.5
29	3.0
30	5.0
31	3.5
32	4.0
33	6.5
34	10.0
35	13.0
36	24.5
37	38.5
38	44.5
39	60.5
40	79.0
41	82.5
42	90.5
43	117.5
44	134.0
45	144.0
46	153.0
47	154.0
48	157.5
49	145.5
50	134.0
51	128.5
52	121.0
53	112.0
54	97.0
55	89.0
56	96.5
57	98.5
58	85.5
59	76.5
60	76.5
61	80.0
62	86.0
63	94.0
64	88.0
65	88.0
66	93.5
67	85.5
68	86.5
69	91.5
70	85.0
71	74.0
72	71.5
73	67.5
74	60.0
75	52.0
76	41.0
77	34.0
78	30.0
79	23.0
80	20.5
81	19.5
82	10.5
83	6.0
84	5.0
85	4.0
86	3.5
87	3.5
88	3.0
89	1.5
90	0.0
91	0.5
92	0.5
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.5
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	5.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
150	4000.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	99.85000000000001
#Duplication Level	Percentage of deduplicated	Percentage of total
1	99.84977466199298	99.7
2	0.15022533800701052	0.3
3	0.0	0.0
4	0.0	0.0
5	0.0	0.0
6	0.0	0.0
7	0.0	0.0
8	0.0	0.0
9	0.0	0.0
>10	0.0	0.0
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	0.0	0.0
20-21	0.0	0.0	0.0	0.0	0.0
22-23	0.0	0.0	0.0	0.0	0.0
24-25	0.0	0.0	0.0	0.0	0.0
26-27	0.0	0.0	0.0	0.0	0.0
28-29	0.0	0.0	0.0	0.0	0.0
30-31	0.0	0.0	0.0	0.0	0.0
32-33	0.0	0.0	0.0	0.0	0.0
34-35	0.0	0.0	0.0	0.0	0.0
36-37	0.0	0.0	0.0	0.0	0.0
38-39	0.0	0.0	0.0	0.0	0.0
40-41	0.0	0.0	0.0	0.0	0.0
42-43	0.0	0.0	0.0	0.0	0.0
44-45	0.0	0.0	0.0	0.0	0.0
46-47	0.0	0.0	0.0	0.0	0.0
48-49	0.0	0.0	0.0	0.0	0.0
50-51	0.0	0.0	0.0	0.0	0.0
52-53	0.0	0.0	0.0	0.0	0.0
54-55	0.0	0.0	0.0	0.0	0.0
56-57	0.0	0.0	0.0	0.0	0.0
58-59	0.0	0.0	0.0	0.0	0.0
60-61	0.0	0.0	0.0	0.0	0.0
62-63	0.0	0.0	0.0	0.0	0.0
64-65	0.0	0.0	0.0	0.0	0.0
66-67	0.0	0.0	0.0	0.0	0.0
68-69	0.0	0.0	0.0	0.0	0.0
70-71	0.0	0.0	0.0	0.0	0.0
72-73	0.0	0.0	0.0	0.0	0.0
74-75	0.0	0.0	0.0	0.0	0.0
76-77	0.0	0.0	0.0	0.0	0.0
78-79	0.0	0.0	0.0	0.0	0.0
80-81	0.0	0.0	0.0	0.0	0.0
82-83	0.0	0.0	0.0	0.0	0.0
84-85	0.0	0.0	0.0	0.0	0.0
86-87	0.0	0.0	0.0	0.0	0.0
88-89	0.0	0.0	0.0	0.0	0.0
90-91	0.0	0.0	0.0	0.0	0.0
92-93	0.0	0.0	0.0	0.0	0.0
94-95	0.0	0.0	0.0	0.0	0.0
96-97	0.0	0.0	0.0	0.0	0.0
98-99	0.0	0.0	0.0	0.0	0.0
100-101	0.0	0.0	0.0	0.0	0.0
102-103	0.0	0.0	0.0	0.0	0.0
104-105	0.0	0.0	0.0	0.0	0.0
106-107	0.0	0.0	0.0	0.0	0.0
108-109	0.0	0.0	0.0	0.0	0.0
110-111	0.0	0.0	0.0	0.0	0.0
112-113	0.025	0.0	0.0	0.0	0.0
114-115	0.05	0.0	0.0	0.0	0.0
116-117	0.1	0.0	0.0	0.0	0.0
118-119	0.15	0.0	0.0	0.0	0.0
120-121	0.25	0.0	0.0	0.0	0.0
122-123	0.35	0.0	0.0	0.0	0.0
124-125	0.35	0.0	0.0	0.0	0.0
126-127	0.35	0.0	0.0	0.0	0.0
128-129	0.47500000000000003	0.0	0.0	0.0	0.0
130-131	0.6499999999999999	0.0	0.0	0.0	0.0
132-133	0.975	0.0	0.0	0.0	0.0
134-135	1.4125	0.0	0.0	0.0	0.0
136-137	1.75	0.0	0.0	0.0	0.0
138	2.2	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE
Read 2635633 spots for SRR6322447.sra
Written 2635633 spots for SRR6322447.sra
Read 2635633 spots for SRR6322447.sra
Written 2635633 spots for SRR6322447.sra
Read 2635633 spots for SRR6322447.sra
Written 2635633 spots for SRR6322447.sra
Read 2635633 spots for SRR6322447.sra
Written 2635633 spots for SRR6322447.sra
Read 2635633 spots for SRR6322447.sra
Written 2635633 spots for SRR6322447.sra
Read 2635633 spots for SRR6322447.sra
Written 2635633 spots for SRR6322447.sra
Read 2635633 spots for SRR6322447.sra
Written 2635633 spots for SRR6322447.sra
Read 2635633 spots for SRR6322447.sra
Written 2635633 spots for SRR6322447.sra
Read 2635633 spots for SRR6322447.sra
Written 2635633 spots for SRR6322447.sra
Read 2635633 spots for SRR6322447.sra
Written 2635633 spots for SRR6322447.sra
Read 2635633 spots for SRR6322447.sra
Written 2635633 spots for SRR6322447.sra
Read 2635633 spots for SRR6322447.sra
Written 2635633 spots for SRR6322447.sra
Read 2635633 spots for SRR6322447.sra
Written 2635633 spots for SRR6322447.sra
Read 2635651 spots for SRR6322447.sra
Written 2635651 spots for SRR6322447.sra
Read 2635633 spots for SRR6322447.sra
Written 2635633 spots for SRR6322447.sra
Read 2635633 spots for SRR6322447.sra
Written 2635633 spots for SRR6322447.sra
Read 2635633 spots for SRR6322447.sra
Written 2635633 spots for SRR6322447.sra
Read 2635633 spots for SRR6322447.sra
Written 2635633 spots for SRR6322447.sra
Read 2635633 spots for SRR6322447.sra
Written 2635633 spots for SRR6322447.sra
Read 2635633 spots for SRR6322447.sra
Written 2635633 spots for SRR6322447.sra
SRR ids: ['SRR6322447.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_8jpk4og_
SRR6322447.sra spots: 52712678
blocks: [[1, 2635633], [2635634, 5271266], [5271267, 7906899], [7906900, 10542532], [10542533, 13178165], [13178166, 15813798], [15813799, 18449431], [18449432, 21085064], [21085065, 23720697], [23720698, 26356330], [26356331, 28991963], [28991964, 31627596], [31627597, 34263229], [34263230, 36898862], [36898863, 39534495], [39534496, 42170128], [42170129, 44805761], [44805762, 47441394], [47441395, 50077027], [50077028, 52712678]]
SRR6322447 file size 19397624
SRR6322447 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR6322447 SRR6322447_1.fastq
Input file:	SRR6322447_1.fastq
trimmed:	SRR6322447-trimmed.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- minimum overlap length for adapter detection (-k):	inf
-- number of concurrent threads (-t):	20
Sat Dec  7 11:03:43 2024 >> started

Sat Dec  7 11:04:31 2024 >> done (48.071s)
52712678 reads processed; of these:
   63136 ( 0.12%) short reads filtered out after trimming by size control
   49657 ( 0.09%) empty reads filtered out after trimming by size control
52599885 (99.79%) reads available; of these:
27792805 (52.84%) trimmed reads available after processing
24807080 (47.16%) untrimmed reads available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	    7310	  0.01%
 19	    7794	  0.01%
 20	    8528	  0.02%
 21	    9162	  0.02%
 22	    9382	  0.02%
 23	   10404	  0.02%
 24	   10994	  0.02%
 25	   12255	  0.02%
 26	   14558	  0.03%
 27	   13580	  0.03%
 28	   13964	  0.03%
 29	   15038	  0.03%
 30	   14713	  0.03%
 31	   15578	  0.03%
 32	   16007	  0.03%
 33	   16510	  0.03%
 34	   16832	  0.03%
 35	   17111	  0.03%
 36	   17298	  0.03%
 37	   17968	  0.03%
 38	   18187	  0.03%
 39	   19416	  0.04%
 40	   19111	  0.04%
 41	   19884	  0.04%
 42	   20549	  0.04%
 43	   21186	  0.04%
 44	   21444	  0.04%
 45	   21654	  0.04%
 46	   23170	  0.04%
 47	   23989	  0.05%
 48	   23943	  0.05%
 49	   24160	  0.05%
 50	   24610	  0.05%
 51	   20895	  0.04%
 52	   20700	  0.04%
 53	   22288	  0.04%
 54	   22300	  0.04%
 55	   22816	  0.04%
 56	   23146	  0.04%
 57	   23712	  0.05%
 58	   23270	  0.04%
 59	   23571	  0.04%
 60	   24146	  0.05%
 61	   25705	  0.05%
 62	   26691	  0.05%
 63	   27510	  0.05%
 64	   29046	  0.06%
 65	   33499	  0.06%
 66	   34680	  0.07%
 67	   34336	  0.07%
 68	   34876	  0.07%
 69	   34966	  0.07%
 70	   35576	  0.07%
 71	   38693	  0.07%
 72	   40543	  0.08%
 73	   42009	  0.08%
 74	   43030	  0.08%
 75	   43444	  0.08%
 76	   38568	  0.07%
 77	   40596	  0.08%
 78	   45533	  0.09%
 79	   47552	  0.09%
 80	   50622	  0.10%
 81	   55153	  0.10%
 82	   56991	  0.11%
 83	   59183	  0.11%
 84	   61629	  0.12%
 85	   64623	  0.12%
 86	   66592	  0.13%
 87	   70318	  0.13%
 88	   71209	  0.14%
 89	   73545	  0.14%
 90	   77199	  0.15%
 91	   79840	  0.15%
 92	   82501	  0.16%
 93	   86407	  0.16%
 94	   90973	  0.17%
 95	   95504	  0.18%
 96	   99818	  0.19%
 97	  104556	  0.20%
 98	  108435	  0.21%
 99	  111973	  0.21%
100	  116949	  0.22%
101	  123192	  0.23%
102	  128446	  0.24%
103	  131394	  0.25%
104	  138080	  0.26%
105	  143652	  0.27%
106	  147867	  0.28%
107	  154700	  0.29%
108	  158708	  0.30%
109	  165921	  0.32%
110	  177040	  0.34%
111	  182884	  0.35%
112	  192875	  0.37%
113	  202469	  0.38%
114	  214938	  0.41%
115	  229858	  0.44%
116	  245761	  0.47%
117	  251599	  0.48%
118	  246925	  0.47%
119	  247711	  0.47%
120	  254628	  0.48%
121	  258865	  0.49%
122	  269860	  0.51%
123	  274454	  0.52%
124	  283075	  0.54%
125	  296547	  0.56%
126	  302597	  0.58%
127	  311222	  0.59%
128	  330391	  0.63%
129	  342051	  0.65%
130	  359290	  0.68%
131	  381050	  0.72%
132	  396159	  0.75%
133	  420353	  0.80%
134	  441093	  0.84%
135	  452720	  0.86%
136	  464828	  0.88%
137	  491659	  0.93%
138	  516043	  0.98%
139	  551291	  1.05%
140	  603383	  1.15%
141	  655489	  1.25%
142	  724584	  1.38%
143	  813987	  1.55%
144	  918193	  1.75%
145	 1068545	  2.03%
146	 1289608	  2.45%
147	 1537347	  2.92%
148	 1952329	  3.71%
149	 4193138	  7.97%
150	24807080	 47.16%
52599885 reads passed initial QC


criterion=sequence-density
sequence-density=1.20
sequence-density-rank=1
fanout-score=71.84
fanout-score-rank=9
prefix-density=1.83
prefix-fanout=47.3
sequence=AGATCGGAAGAGC


criterion=fanout-score
sequence-density=0.10
sequence-density-rank=22
fanout-score=351.50
fanout-score-rank=1
prefix-density=1.29
prefix-fanout=26.4
sequence=CGGCGGCGGCGA
                                 Started job on |	Dec 07 11:04:51
                             Started mapping on |	Dec 07 11:04:51
                                    Finished on |	Dec 07 11:06:11
       Mapping speed, Million of reads per hour |	2366.99

                          Number of input reads |	52599885
                      Average input read length |	139
                                    UNIQUE READS:
                   Uniquely mapped reads number |	50099387
                        Uniquely mapped reads % |	95.25%
                          Average mapped length |	138.61
                       Number of splices: Total |	18809110
            Number of splices: Annotated (sjdb) |	17758395
                       Number of splices: GT/AG |	18535459
                       Number of splices: GC/AG |	221315
                       Number of splices: AT/AC |	11306
               Number of splices: Non-canonical |	41030
                      Mismatch rate per base, % |	0.29%
                         Deletion rate per base |	0.02%
                        Deletion average length |	2.45
                        Insertion rate per base |	0.01%
                       Insertion average length |	2.20
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	899132
             % of reads mapped to multiple loci |	1.71%
        Number of reads mapped to too many loci |	1133422
             % of reads mapped to too many loci |	2.15%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	0.61%
                     % of reads unmapped: other |	0.28%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	1601366	1601366	1601366
N_multimapping	899132	899132	899132
N_noFeature	1152215	25328056	25229851
N_ambiguous	788754	51156	53773
UnstrandedReadsAssigned:48158418 PositiveStrandReadsAssigned:24720175 NegativeStrandReadsAssigned:24815763
Dataset is classified unstranded
MeadianReadLen=150 20thPercentileLength=143 echo kmer=139
SRR6322447 Starting Kallisto single end mapping to ensembl reference transcriptome. kmer=31

[quant] fragment length distribution is truncated gaussian with mean = 100, sd = 20
[index] k-mer length: 31
[index] number of targets: 52,972
[index] number of k-mers: 66,720,672
[index] number of equivalence classes: 111,837
[quant] running in single-end mode
[quant] will process file 1: SRR6322447-trimmed.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 52,599,885 reads, 49,487,357 reads pseudoaligned
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 1,163 rounds

  52973 SRR6322447.ke.tsv
  35125 SRR6322447.se.tsv
  88098 total
==> SRR6322447.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
PNS24245	936	837	13.4803	0.512171
PNS24247	1044	945	115.02	3.87066
PNS24249	1928	1829	1123.67	19.5374
PNS24246	1044	945	115.02	3.87066
PNS24248	1044	945	115.02	3.87066
PNS24244	1471	1372	84.7887	1.96529
PNS24243	293	194	29	4.75377
KQK14069	1603	1504	11871.2	251.008
KQK14071	474	375	2172.55	184.239

==> SRR6322447.se.tsv <==
BRADI_1g14170v3	14291
BRADI_1g53295v3	481
BRADI_1g59795v3	520
BRADI_1g07683v3	0
BRADI_1g00485v3	69
BRADI_1g20270v3	1241
BRADI_1g74790v3	1472
BRADI_1g09890v3	0
BRADI_1g77505v3	298
BRADI_1g48960v3	0
SRR6322447 completed mapping pipeline successfully
