Starting /dee2/code/volunteer_pipeline.sh SRR6941534 current disk space = 1551861256192 free memory = 1601460484 SRR6941534 SRAfilesize b6a11dd2cfc9d9d19befae3530eb8836 SRR6941534.sra SRR6941534.sra file validated SRR6941534 is paired end SRR6941534 is conventional basespace SRR6941534 read1 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR6941534_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 50 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 34.418 35.0 35.0 35.0 33.0 35.0 2 34.51175 35.0 35.0 35.0 34.0 35.0 3 34.64075 35.0 35.0 35.0 35.0 35.0 4 34.67575 35.0 35.0 35.0 35.0 35.0 5 34.53825 35.0 35.0 35.0 35.0 35.0 6 39.3245 40.0 40.0 40.0 39.0 40.0 7 39.30725 40.0 40.0 40.0 39.0 40.0 8 39.21975 40.0 40.0 40.0 39.0 40.0 9 39.42075 40.0 40.0 40.0 39.0 40.0 10-14 39.3711 40.0 40.0 40.0 39.0 40.0 15-19 39.2226 40.0 40.0 40.0 38.8 40.0 20-24 39.319849999999995 40.0 40.0 40.0 39.0 40.0 25-29 39.33595 40.0 40.0 40.0 39.0 40.0 30-34 39.2427 40.0 40.0 40.0 38.8 40.0 35-39 39.317400000000006 40.0 40.0 40.0 39.0 40.0 40-44 39.31985 40.0 40.0 40.0 39.0 40.0 45-49 39.24575 40.0 40.0 40.0 39.0 40.0 50-54 39.20545 40.0 40.0 40.0 38.8 40.0 55-59 39.2159 40.0 40.0 40.0 38.8 40.0 60-64 39.161199999999994 40.0 40.0 40.0 38.6 40.0 65-69 39.19985 40.0 40.0 40.0 39.0 40.0 70-74 39.16145 40.0 40.0 40.0 38.4 40.0 75-79 39.0342 40.0 39.8 40.0 37.8 40.0 80-84 39.10455 40.0 40.0 40.0 38.0 40.0 85-89 38.9261 40.0 39.0 40.0 37.8 40.0 90-94 39.0244 40.0 39.6 40.0 38.0 40.0 95-99 38.84625000000001 40.0 39.0 40.0 37.2 40.0 100-104 38.31355 39.4 38.4 39.8 36.2 39.8 105-109 38.864999999999995 40.0 39.0 40.0 37.0 40.0 110-114 38.864999999999995 40.0 39.0 40.0 37.2 40.0 115-119 38.8565 40.0 39.0 40.0 37.0 40.0 120-124 38.70620000000001 40.0 39.0 40.0 36.2 40.0 125-129 38.54625 40.0 39.0 40.0 36.0 40.0 130-134 38.438849999999995 40.0 39.0 40.0 36.0 40.0 135-139 38.371050000000004 40.0 39.0 40.0 35.8 40.0 140-144 38.189750000000004 40.0 39.0 40.0 35.8 40.0 145-149 37.674099999999996 40.0 39.0 40.0 34.6 40.0 150-151 34.873374999999996 38.5 35.0 39.5 24.5 40.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 9 1.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 1.0 19 0.0 20 0.0 21 1.0 22 1.0 23 2.0 24 2.0 25 8.0 26 5.0 27 16.0 28 13.0 29 24.0 30 15.0 31 29.0 32 41.0 33 41.0 34 69.0 35 97.0 36 105.0 37 186.0 38 323.0 39 3020.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 51.99098422238918 8.039068369646882 3.931880791384924 36.03806661657901 2 20.265531062124246 7.790581162324648 32.86573146292585 39.078156312625254 3 19.975 13.100000000000001 26.025 40.9 4 26.85 20.525 20.375 32.25 5 25.09400852343946 26.57307595888694 25.14414640260717 23.18876911506643 6 23.75 27.650000000000002 24.8 23.799999999999997 7 16.075 23.799999999999997 41.025 19.1 8 18.625 21.95 31.574999999999996 27.85 9 20.424999999999997 18.95 35.475 25.15 10-14 21.65 24.59 26.945000000000004 26.815 15-19 22.725 24.92 24.975 27.38 20-24 21.705 23.925 26.625 27.744999999999997 25-29 22.64 22.84 27.18 27.339999999999996 30-34 23.150000000000002 23.849999999999998 25.405 27.595 35-39 22.685 21.89 27.235 28.189999999999998 40-44 22.07 23.965 25.430000000000003 28.535 45-49 22.884999999999998 21.45 26.83 28.835 50-54 23.189999999999998 22.375 26.325 28.110000000000003 55-59 21.81 23.395 27.634999999999998 27.16 60-64 23.830000000000002 22.445 26.27 27.455000000000002 65-69 22.189999999999998 24.75 25.995 27.065 70-74 22.16 24.265 25.435000000000002 28.139999999999997 75-79 22.96 24.035 26.125 26.88 80-84 22.3 25.715 25.025 26.96 85-89 23.195 24.654999999999998 25.215 26.935 90-94 23.73 24.26 24.295 27.715 95-99 23.73 24.235 24.48 27.555000000000003 100-104 22.939999999999998 24.12 24.955 27.985 105-109 22.48 23.369999999999997 25.72 28.43 110-114 22.715 24.66 25.724999999999998 26.900000000000002 115-119 22.595000000000002 24.759999999999998 24.775 27.87 120-124 22.71 25.965 23.59 27.735 125-129 22.27 24.725 24.709999999999997 28.294999999999998 130-134 23.995 23.56 23.515 28.93 135-139 21.815 24.935 24.42 28.83 140-144 23.455000000000002 24.36 24.095 28.09 145-149 21.404999999999998 25.474999999999998 25.05 28.07 150-151 21.86366479049406 24.915572232645403 24.72795497185741 28.492808005003127 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.5 20 0.5 21 0.5 22 2.5 23 3.0 24 3.5 25 4.0 26 5.5 27 6.0 28 4.5 29 3.5 30 5.5 31 9.5 32 12.0 33 14.5 34 17.0 35 24.0 36 42.0 37 68.0 38 92.5 39 116.0 40 120.5 41 111.5 42 90.5 43 85.0 44 99.5 45 111.0 46 114.0 47 95.5 48 80.5 49 85.0 50 99.5 51 123.5 52 141.5 53 175.5 54 235.5 55 368.5 56 392.5 57 249.5 58 186.0 59 177.0 60 131.5 61 76.0 62 47.5 63 42.0 64 32.0 65 18.0 66 14.0 67 5.5 68 7.5 69 7.0 70 5.0 71 5.5 72 8.0 73 8.5 74 4.0 75 3.5 76 4.0 77 2.5 78 1.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.17500000000000002 2 0.2 3 0.0 4 0.0 5 0.27499999999999997 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0625 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.475 #Duplication Level Percentage of deduplicated Percentage of total 1 76.05814450619923 44.474999999999994 2 11.970927746900385 14.000000000000002 3 4.7883710987601535 8.4 4 2.5651988029072252 6.0 5 0.7695596408721677 2.25 6 1.0260795211628901 3.5999999999999996 7 0.6840530141085934 2.8000000000000003 8 0.38477982043608383 1.7999999999999998 9 0.3420265070542967 1.7999999999999998 >10 1.3681060282171869 13.175 >50 0.04275331338178709 1.7000000000000002 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GGCATGATGACTTGGCCTCATCCTCTCCTTCCTCCGGCTTAACACCGGCG 68 1.7000000000000002 No Hit GGGGCATGATGACTTGGCCTCATCCTCTCCTTCCTCCGGCTTAACACCGG 34 0.8500000000000001 No Hit GTGCTCTGCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTCCTA 32 0.8 No Hit CCCGTCAATTCCTTTGAGTTTCATTCTTGCGAACGTACTCCCCAGGCGGG 26 0.65 No Hit CCCCACTGCTGCCTCCCGTAGGAGTCTGGGCCGTGTCTCAGTCCCAGTGT 26 0.65 No Hit GTCCAGTCAACTGCTGCGCCTCAACGCATTTCGGGGAGAACCAGCTAGCT 23 0.575 No Hit CCGGGAACGGATTCACCGCCGTATGGCTGACCGGCGATTACTAGCGATTC 23 0.575 No Hit GTCGGTTTCGGGTACAGGTACCCTTTTGTTGAAGGTCGTTCGAGCTTTTC 22 0.5499999999999999 No Hit GCGGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTACG 22 0.5499999999999999 No Hit CCCACTGCTGCCTCCCGTAGGAGTCTGGGCCGTGTCTCAGTCCCAGTGTG 18 0.44999999999999996 No Hit CTCCACGCGGCATTGCTCCGTCAGGCTTTCGCCCATTGCGGAAAATTCCC 18 0.44999999999999996 No Hit GCTCTGCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTCCTAAA 18 0.44999999999999996 No Hit GGCCAAAATAACCATGAGCAGCCACAATATTATAAGTCTCTTCCTCTTGA 17 0.42500000000000004 No Hit GTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTC 17 0.42500000000000004 No Hit GCCCAATCATTCCGGATAACGCTTGCATCCTCTGTCTTACCGCGGCTGCT 16 0.4 No Hit GCTCCTCAGCCTACGGGGTATTAGCAACCGTTTCCAGTTGTTGTTCCCCT 15 0.375 No Hit GTTCTATTTCACTACCCACTGGGGGTTCTTTTCACCTTTCCCTCACGGTA 14 0.35000000000000003 No Hit CCTCAGCCTACGGGGTATTAGCAACCGTTTCCAGTTGTTGTTCCCCTCCC 14 0.35000000000000003 No Hit GTCAATTCCTTTGAGTTTCATTCTTGCGAACGTACTCCCCAGGCGGGATA 14 0.35000000000000003 No Hit GCCAAAATAACCATGAGCAGCCACAATATTATAAGTCTCTTCCTCTTGAC 14 0.35000000000000003 No Hit GCCACCTACAGACGCTTTACGCCCAATCATTCCGGATAACGCTTGCATCC 13 0.325 No Hit GTTCGAGCTTTTCCTGGGAGTATGGCATCGGTTACATACTTCAGTGCCGT 12 0.3 No Hit GCTTTCTTTTCCTCTGGCTACTAAGATGTTTCAGTTCGCCAGGTTGTCTC 12 0.3 No Hit CCTGTGTCGGTTTCGGGTACAGGTACCCTTTTGTTGAAGGTCGTTCGAGC 12 0.3 No Hit GTCATTGTTTCTTCTCCGAGAAAAGAAGTTGACGACCCGTAGGCCTTCCA 11 0.27499999999999997 No Hit GGCGATAATAAATACAGAAGTTGCGGTCAATAAGGTAGGGATCATCAAAA 11 0.27499999999999997 No Hit GTGAGCATTACGTTCGTGCATTACTTCCATACCAAGATTAGCACGGTTGA 11 0.27499999999999997 No Hit GTCCCAGTGTGGCTGATCATCCTCTCGGACCAGCTACTGATCATCGCCTT 11 0.27499999999999997 No Hit CCTAGCTTTCGTCTCTCAGTGTCAGTGTCGGCCCAGCAGAGTGCTTTCGC 11 0.27499999999999997 No Hit GGAGGGGCTGCGATGAAGGCGATAATAAATACAGAAGTTGCGGTCAATAA 10 0.25 No Hit CGTCAATTCCTTTGAGTTTCATTCTTGCGAACGTACTCCCCAGGCGGGAT 10 0.25 No Hit CCACAATATTATAAGTCTCTTCCTCTTGACCAAATTTGTAACCCTCATTA 10 0.25 No Hit GCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTCCTAAAGGCAT 10 0.25 No Hit GCCGAAAACACCAGCTACACCTAACATGTGAAATGGATGCATAAGGATGT 9 0.22499999999999998 No Hit GATGACTTGGCCTCATCCTCTCCTTCCTCCGGCTTAACACCGGCGGTCTG 9 0.22499999999999998 No Hit GTCCCTATTAATCATTACTCCGATCCCGAAGGCCAACACAATAGGACCGG 9 0.22499999999999998 No Hit GTACAAGGCCCGGGAACGGATTCACCGCCGTATGGCTGACCGGCGATTAC 9 0.22499999999999998 No Hit GTTGATGATATCAGCCCAAGTATTAATAACGCGACCTTGACTATCAACTA 9 0.22499999999999998 No Hit GTCGGTTCGGACCTCTGCTTAGTTTCATCCAAGCTTCATCCTGGTCATGG 9 0.22499999999999998 No Hit CCGCATTAATGGGCGAACAGCCCAACCCTTGGAACCACCTACAGCTCCAG 9 0.22499999999999998 No Hit CTTTCTTTTCCTCTGGCTACTAAGATGTTTCAGTTCGCCAGGTTGTCTCT 9 0.22499999999999998 No Hit CCTTTATCACTGAGCGGTCATTTAGGGGCCTTAGCTGGTGATCCGGGCTG 8 0.2 No Hit CCTTAAACCTATAACCATCTTTCGGCTAACCTAGCCTCCTCCGTCCCTCC 8 0.2 No Hit GGGCATGATGACTTGGCCTCATCCTCTCCTTCCTCCGGCTTAACACCGGC 8 0.2 No Hit GCTGCGATGAAGGCGATAATAAATACAGAAGTTGCGGTCAATAAGGTAGG 8 0.2 No Hit ATCGTTTACGGCTAGGACTACTGGGGTCTCTAATCCCATTTGCTCCCCTA 8 0.2 No Hit CCGGCGATTACTAGCGATTCCTGCTTCATGCAGGCGAGTTGCAGCCTGCA 8 0.2 No Hit GCCCTATGAAGACTCGCTTTCGCTACGGCTCCGGTGGGTTCCGTTCCCTT 8 0.2 No Hit GGTCGTTCGAGCTTTTCCTGGGAGTATGGCATCGGTTACATACTTCAGTG 8 0.2 No Hit GGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTACGTT 8 0.2 No Hit CGGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTACGT 7 0.17500000000000002 No Hit CCTAGATGTCCAGTCAACTGCTGCGCCTCAACGCATTTCGGGGAGAACCA 7 0.17500000000000002 No Hit CCGTCAATTCCTTTGAGTTTCATTCTTGCGAACGTACTCCCCAGGCGGGA 7 0.17500000000000002 No Hit GTGTCGGTTTCGGGTACAGGTACCCTTTTGTTGAAGGTCGTTCGAGCTTT 7 0.17500000000000002 No Hit CCCACCTGTGTCGGTTTCGGGTACAGGTACCCTTTTGTTGAAGGTCGTTC 7 0.17500000000000002 No Hit CCTAGAGTAACTTTTATCCGTTGAGCGACGGCCCTTCCACTCGGCACCGT 7 0.17500000000000002 No Hit CCATCGTTTACGGCTAGGACTACTGGGGTCTCTAATCCCATTTGCTCCCC 7 0.17500000000000002 No Hit ACATGCTCCACCGCTTGTGCGGGCCCCCGTCAATTCCTTTGAGTTTCATT 7 0.17500000000000002 No Hit GCCCCATAGAAACTGTCTACCTGAGACTGTCCCTTGGCCCGCGGGTCTGA 7 0.17500000000000002 No Hit CCTCGCGGTACTTGTTCGCTATCGGTCTCTCGCCTGTATTTAGCCTTGGA 7 0.17500000000000002 No Hit CCCTACCGTACTCCAGCTTGGTAGTTTCCACCGCCTGTCCAGGGTTGAGC 7 0.17500000000000002 No Hit GTTCTTGATTAATGAAAACATCCTTGGCAAATGCTTTCGCAGTTGTTCGT 7 0.17500000000000002 No Hit GTTCCGTTCCCTTAACCAAGCCACTGCCTATGAGTCGCCGGCTCATTCTT 7 0.17500000000000002 No Hit CCCTAGAGTAACTTTTATCCGTTGAGCGACGGCCCTTCCACTCGGCACCG 7 0.17500000000000002 No Hit GCTCATTCTTCAACAGGCACGCGGTCAGAGATCACTTTCCCCTCCCACTG 7 0.17500000000000002 No Hit GTCCTTAAACCTATAACCATCTTTCGGCTAACCTAGCCTCCTCCGTCCCT 7 0.17500000000000002 No Hit GCGGTCAATAAGGTAGGGATCATCAAAACACCGAACCATCCGATGTAAAG 6 0.15 No Hit CCACTGCTGCCTCCCGTAGGAGTCTGGGCCGTGTCTCAGTCCCAGTGTGG 6 0.15 No Hit CTTGTGTCCTTAAACCTATAACCATCTTTCGGCTAACCTAGCCTCCTCCG 6 0.15 No Hit GGGCGGTGTGTACAAGGCCCGGGAACGGATTCACCGCCGTATGGCTGACC 6 0.15 No Hit GGCGGTGTGTACAAAGGGCAGGGACGTAGTCAACGCGAGCTGATGACTCG 6 0.15 No Hit CTCCTCAGCCTACGGGGTATTAGCAACCGTTTCCAGTTGTTGTTCCCCTC 6 0.15 No Hit GGCATAAGGGGCATGATGACTTGGCCTCATCCTCTCCTTCCTCCGGCTTA 6 0.15 No Hit GTCTGACACAAGGTTAGAATCCGAGCTCTTCCAGAGTGGTATCTCACTGA 6 0.15 No Hit GTCGGCATCGTTTATGGTTGAGACTAGGACGGTATCTGATCGTCTTCGAG 6 0.15 No Hit GGGCGGTGTGTACAAAGGGCAGGGACGTAGTCAACGCGAGCTGATGACTC 6 0.15 No Hit CGGGAACGGATTCACCGCCGTATGGCTGACCGGCGATTACTAGCGATTCC 6 0.15 No Hit GTCTCTCAGTGTCAGTGTCGGCCCAGCAGAGTGCTTTCGCCGTTGGTGTT 6 0.15 No Hit GCATGATGACTTGGCCTCATCCTCTCCTTCCTCCGGCTTAACACCGGCGG 6 0.15 No Hit GTCGAGTTATCATGAATCATCGGATCAGCGAGCAAAGCCCGCGTCAGCCT 6 0.15 No Hit GTCGCACAGCACCTAGTATCCATCGTTTACGGCTAGGACTACTGGGGTCT 6 0.15 No Hit CTCCTTTTGCTCCTCAGCCTACGGGGTATTAGCAACCGTTTCCAGTTGTT 6 0.15 No Hit GTCGTTCGAGCTTTTCCTGGGAGTATGGCATCGGTTACATACTTCAGTGC 6 0.15 No Hit GCTCACGTACCGCATTAATGGGCGAACAGCCCAACCCTTGGAACCACCTA 6 0.15 No Hit CAGGGTTCCAAACTCATAGTGGCAACTAAACACGAGGGTTGCGCTCGTTG 6 0.15 No Hit CTCTTGACCAAATTTGTAACCCTCATTAGCAGATTCATTTTCAGTAGTTT 6 0.15 No Hit GTCAATAAGGTAGGGATCATCAAAACACCGAACCATCCGATGTAAAGACG 6 0.15 No Hit ATCGAATTAAACCACATGCTCCACCGCTTGTGCGGGCCCCCGTCAATTCC 6 0.15 No Hit CTCTGCCCCTACCGTACTCCAGCTTGGTAGTTTCCACCGCCTGTCCAGGG 6 0.15 No Hit CTCGCGAATACCATCAATATCTACTGGAGGGGCTGCGATGAAGGCGATAA 6 0.15 No Hit GCCTGTTGTCCATCGACTACGCCTTTCGGCCTGATCTTAGGCCCTGACTC 5 0.125 No Hit GTGTGTACAAAGGGCAGGGACGTAGTCAACGCGAGCTGATGACTCGCGCT 5 0.125 No Hit CTTGACTATCAACTACAGATTGGTTGAAATTGAATCCATTTAGGTTGAAC 5 0.125 No Hit GCTGCCTCCCGTAGGAGTCTGGGCCGTGTCTCAGTCCCAGTGTGGCTGAT 5 0.125 No Hit CCTCTCAATGCTCTAACGCCCACACCGGATATGGACCGAACTGTCTCACG 5 0.125 No Hit GTCAGTGTCGGCCCAGCAGAGTGCTTTCGCCGTTGGTGTTCTTTCCGATC 5 0.125 No Hit CCCACAACCCCGTTTTCACGGTTTAGGCTGCTCCCATTTCGCTCGCCGCT 5 0.125 No Hit GTACAAAGGGCAGGGACGTAGTCAACGCGAGCTGATGACTCGCGCTTACT 5 0.125 No Hit GCTTTGAGCACTCTAATTTCTTCAAAGTAACGATGCCGGAGGCACGACCC 5 0.125 No Hit CCTAGGTATTCTCTACCTACCCACCTGTGTCGGTTTCGGGTACAGGTACC 5 0.125 No Hit CCGGCTTCCGGTTCATCCCGCATCGCCAGTTCTGCTTACCAAAAATGGCC 5 0.125 No Hit GGGCTTACTACTTATATGCTTTCAGCAGTTATCCTCTCCGCACTTGGCTA 5 0.125 No Hit CTCAGATACCGTCATTGTTTCTTCTCCGAGAAAAGAAGTTGACGACCCGT 5 0.125 No Hit GTCAACTGCTGCGCCTCAACGCATTTCGGGGAGAACCAGCTAGCTCTGGG 5 0.125 No Hit GCCCCTCCTTGGGCGGATTTCTCCTTTTGCTCCTCAGCCTACGGGGTATT 5 0.125 No Hit CCACGCGGCATTGCTCCGTCAGGCTTTCGCCCATTGCGGAAAATTCCCCA 5 0.125 No Hit GTCACCCAGGAGTATTTAGCCTTGCAAGGTGGTCCTTGCTGATTCACACG 5 0.125 No Hit GTTTATGGTTGAGACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACT 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0125 0.0 0.0 0.0 0.0 48-49 0.025 0.0 0.0 0.0 0.0 50-51 0.037500000000000006 0.0 0.0 0.0 0.0 52-53 0.05 0.0 0.0 0.0 0.0 54-55 0.05 0.0 0.0 0.0 0.0 56-57 0.05 0.0 0.0 0.0 0.0 58-59 0.05 0.0 0.0 0.0 0.0 60-61 0.0625 0.0 0.0 0.0 0.0 62-63 0.125 0.0 0.0 0.0 0.0 64-65 0.16249999999999998 0.0 0.0 0.0 0.0 66-67 0.1875 0.0 0.0 0.0 0.0 68-69 0.2875 0.0 0.0 0.0 0.0 70-71 0.3625 0.0 0.0 0.0 0.0 72-73 0.4125 0.0 0.0 0.0 0.0 74-75 0.48750000000000004 0.0 0.0 0.0 0.0 76-77 0.6125 0.0 0.0 0.0 0.0 78-79 0.7124999999999999 0.0 0.0 0.0 0.0 80-81 0.7375 0.0 0.0 0.0 0.0 82-83 0.85 0.0 0.0 0.0 0.0 84-85 0.975 0.0 0.0 0.0 0.0 86-87 1.2125 0.0 0.0 0.0 0.0 88-89 1.4375 0.0 0.0 0.0 0.0 90-91 1.7875 0.0 0.0 0.0 0.0 92-93 2.0125 0.0 0.0 0.0 0.0 94-95 2.2625 0.0 0.0 0.0 0.0 96-97 2.45 0.0 0.0 0.0 0.0 98-99 2.875 0.0 0.0 0.0 0.0 100-101 3.2875 0.0 0.0 0.0 0.0 102-103 3.6500000000000004 0.0 0.0 0.0 0.0 104-105 4.15 0.0 0.0 0.0 0.0 106-107 4.6875 0.0 0.0 0.0 0.0 108-109 5.2125 0.0 0.0 0.0 0.0 110-111 5.8125 0.0 0.0 0.0 0.0 112-113 6.475 0.0 0.0 0.0 0.0 114-115 7.0 0.0 0.0 0.0 0.0 116-117 7.8375 0.0 0.0 0.0 0.0 118-119 8.3875 0.0 0.0 0.0 0.0 120-121 8.9875 0.0 0.0 0.0 0.0 122-123 9.4875 0.0 0.0 0.0 0.0 124-125 10.0125 0.0 0.0 0.0 0.0 126-127 10.975 0.0 0.0 0.0 0.0 128-129 11.600000000000001 0.0 0.0 0.0 0.0 130-131 12.25 0.0 0.0 0.0 0.0 132-133 12.7875 0.0 0.0 0.0 0.0 134-135 13.4875 0.0 0.0 0.0 0.0 136-137 14.037500000000001 0.0 0.0 0.0 0.0 138-139 14.8 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCGGTGT 10 0.0065840036 146.77216 3 CGGTGTG 10 0.0065840036 146.77216 4 GGGCGGT 10 0.0065840036 146.77216 1 GGCGGTG 10 0.0065840036 146.77216 2 GGTGTGT 10 0.0065840036 146.77216 5 GTGTGTA 10 0.0068396386 144.9375 6 >>END_MODULE SRR6941534 read2 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR6941534_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 50 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 34.116 35.0 35.0 35.0 32.0 35.0 2 34.3345 35.0 35.0 35.0 33.0 35.0 3 34.32325 35.0 35.0 35.0 33.0 35.0 4 34.2775 35.0 35.0 35.0 33.0 35.0 5 34.32125 35.0 35.0 35.0 33.0 35.0 6 39.09575 40.0 40.0 40.0 38.0 40.0 7 39.04525 40.0 40.0 40.0 38.0 40.0 8 39.13175 40.0 40.0 40.0 39.0 40.0 9 39.1285 40.0 40.0 40.0 39.0 40.0 10-14 39.135149999999996 40.0 40.0 40.0 38.8 40.0 15-19 39.176500000000004 40.0 40.0 40.0 38.8 40.0 20-24 39.1535 40.0 40.0 40.0 39.0 40.0 25-29 39.151799999999994 40.0 40.0 40.0 38.8 40.0 30-34 39.0778 40.0 40.0 40.0 38.4 40.0 35-39 39.1075 40.0 40.0 40.0 38.8 40.0 40-44 39.0109 40.0 39.8 40.0 38.4 40.0 45-49 38.86665000000001 40.0 39.2 40.0 37.6 40.0 50-54 38.8613 40.0 39.0 40.0 37.6 40.0 55-59 38.933749999999996 40.0 39.6 40.0 38.0 40.0 60-64 38.85965 40.0 39.0 40.0 37.4 40.0 65-69 38.7335 40.0 39.0 40.0 36.8 40.0 70-74 38.729949999999995 40.0 39.0 40.0 36.6 40.0 75-79 38.59225 40.0 39.0 40.0 36.2 40.0 80-84 38.634499999999996 40.0 39.0 40.0 36.6 40.0 85-89 38.53345 40.0 39.0 40.0 36.0 40.0 90-94 38.48375 40.0 39.0 40.0 36.2 40.0 95-99 38.37910000000001 40.0 39.0 40.0 35.8 40.0 100-104 37.518499999999996 39.0 38.0 39.6 34.0 39.8 105-109 38.0988 40.0 39.0 40.0 35.2 40.0 110-114 34.807050000000004 36.4 34.2 38.4 30.2 38.6 115-119 18.8399 16.6 15.8 23.4 13.4 30.0 120-124 2.0 2.0 2.0 2.0 2.0 2.0 125-129 2.0 2.0 2.0 2.0 2.0 2.0 130-134 2.0 2.0 2.0 2.0 2.0 2.0 135-139 2.0 2.0 2.0 2.0 2.0 2.0 140-144 2.0 2.0 2.0 2.0 2.0 2.0 145-149 2.0 2.0 2.0 2.0 2.0 2.0 150-151 2.0 2.0 2.0 2.0 2.0 2.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 0.0 4 0.0 5 1.0 6 0.0 7 0.0 8 1.0 9 1.0 10 0.0 11 0.0 12 2.0 13 1.0 14 3.0 15 3.0 16 4.0 17 6.0 18 11.0 19 13.0 20 19.0 21 19.0 22 26.0 23 21.0 24 30.0 25 60.0 26 70.0 27 94.0 28 157.0 29 295.0 30 2003.0 31 1158.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 54.445279238667666 17.330328074129728 5.835211620335587 22.389181066867017 2 30.17025538307461 22.283425137706562 27.491236855282924 20.055082623935906 3 25.306633291614517 22.703379224030037 32.49061326658323 19.499374217772214 4 27.1407110665999 31.84777165748623 21.532298447671508 19.479218828242363 5 30.67100650976465 31.096644967451176 18.85327991987982 19.379068602904358 6 24.9 36.0 18.775 20.325 7 22.825 20.775 33.875 22.525000000000002 8 26.025 23.35 24.75 25.874999999999996 9 28.375 22.325 24.9 24.4 10-14 28.51 24.97 23.43 23.09 15-19 28.305000000000003 26.075 23.565 22.055 20-24 27.705000000000002 25.44 24.22 22.634999999999998 25-29 28.225 25.319999999999997 23.69 22.765 30-34 28.38 26.395000000000003 22.895 22.33 35-39 28.471423571178562 26.4113205660283 23.481174058702937 21.636081804090203 40-44 28.372837283728376 26.62766276627663 23.072307230723073 21.927192719271925 45-49 28.541385008261983 26.74377847879425 22.938260477692655 21.776576035251114 50-54 28.171128346259692 25.69427070302727 23.55266449837378 22.581936452339253 55-59 28.31189630148641 26.650317801911815 23.25709423952755 21.780691657074218 60-64 28.230526789734355 25.368952924108264 24.10325679123518 22.297263494922205 65-69 29.672418104526134 25.61140285071268 23.515878969742435 21.200300075018756 70-74 28.97948974487244 25.62281140570285 23.716858429214607 21.680840420210103 75-79 29.14 24.975 23.525 22.36 80-84 28.41136454581833 25.185074029611844 24.2296918767507 22.173869547819127 85-89 28.466423321166058 25.12625631281564 23.746187309365467 22.661133056652833 90-94 29.175 25.240000000000002 23.200000000000003 22.384999999999998 95-99 29.049999999999997 25.39 23.315 22.245 100-104 28.04 27.275 22.830000000000002 21.855 105-109 28.775000000000002 25.16 24.27 21.795 110-114 28.700180252353295 26.011415982375325 24.08371720408572 21.20468656118566 115-119 29.386680045152392 25.81211589113257 23.309920983318698 21.491283080396336 120-124 26.69577322196912 41.08453556061756 14.591242723361175 17.628448494052137 125-129 NaN NaN NaN NaN 130-134 28.80080280983442 26.680883090817865 22.240341194179628 22.277972905168088 135-139 28.698922035597896 26.367510654299327 22.702431687139637 22.23113562296315 140-144 30.184998741505158 25.90611628492323 23.559023408004027 20.349861565567583 145-149 31.465732866433214 24.287143571785894 23.51175587793897 20.735367683841922 150-151 29.366866866866864 26.13863863863864 22.084584584584587 22.40990990990991 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.5 19 1.0 20 1.0 21 1.0 22 1.5 23 2.5 24 2.5 25 2.5 26 3.5 27 6.0 28 6.0 29 9.0 30 10.5 31 9.5 32 13.0 33 18.5 34 29.5 35 44.5 36 52.5 37 68.0 38 77.5 39 81.0 40 96.5 41 101.5 42 90.5 43 90.5 44 97.5 45 97.0 46 102.5 47 93.0 48 87.0 49 97.5 50 103.0 51 134.0 52 145.0 53 186.5 54 303.0 55 363.5 56 307.0 57 219.0 58 171.5 59 153.5 60 141.5 61 107.0 62 81.0 63 52.0 64 23.0 65 18.5 66 13.0 67 12.5 68 18.5 69 12.0 70 6.5 71 7.0 72 4.0 73 2.0 74 2.5 75 3.5 76 3.5 77 2.5 78 4.5 79 3.5 80 0.5 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 0.17500000000000002 2 0.15 3 0.125 4 0.15 5 0.15 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.005 40-44 0.01 45-49 0.145 50-54 0.075 55-59 0.095 60-64 0.055 65-69 0.025 70-74 0.05 75-79 0.0 80-84 0.04 85-89 0.005 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.13999999999999999 115-119 20.27 120-124 20.979999999999997 125-129 100.0 130-134 60.14000000000001 135-139 0.27499999999999997 140-144 20.54 145-149 80.01 150-151 0.1 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 63.849999999999994 #Duplication Level Percentage of deduplicated Percentage of total 1 74.9412685982772 47.85 2 14.17384494909945 18.099999999999998 3 4.815974941268599 9.225 4 2.623335943617854 6.7 5 1.5270164447924823 4.875 6 0.4698512137823023 1.7999999999999998 7 0.39154267815191857 1.7500000000000002 8 0.31323414252153486 1.6 9 0.11746280344557558 0.675 >10 0.5873140172278779 6.0249999999999995 >50 0.03915426781519186 1.4000000000000001 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTTCGATCCTGGCTCAGGATGAACGCTGGCGGCATGCTTAACACATGCAA 56 1.4000000000000001 No Hit GGCTTGCGGTGGATACCTAGGCACCCAGAGACGAGGAAGGGCGTAGCAAG 32 0.8 No Hit CCTTACCAGGGCTTGACATGCCGCGAATCCTCTTGAAAGAGAGGGGTGCC 31 0.775 No Hit AGAACCTTACCAGGGCTTGACATGCCGCGAATCCTCTTGAAAGAGAGGGG 22 0.5499999999999999 No Hit GAACCTTACCAGGGCTTGACATGCCGCGAATCCTCTTGAAAGAGAGGGGT 21 0.525 No Hit CAGTGACCAGGCCCGGGCGACTGTTTACCAAAAACACAGGTCTCCGCAAA 20 0.5 No Hit TCTATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGA 15 0.375 No Hit GTTGGGTTAAGTCTCGCAACGAGCGCAACCCTCGTGTTTAGTTGCCACTA 13 0.325 No Hit GCCGCGAATCCTCTTGAAAGAGAGGGGTGCCCTCGGGAACGCGGACACAG 12 0.3 No Hit GTTATTTTGGCCGATTAATCTTCCAATATGCTAGTTTCAACAACTCTCGT 12 0.3 No Hit CTTACCAGGGCTTGACATGCCGCGAATCCTCTTGAAAGAGAGGGGTGCCC 11 0.27499999999999997 No Hit ATTATCTATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAAT 11 0.27499999999999997 No Hit GTTCCGACCCGCACGAAAGGCGTAACGATCTGGGCACTGTCTCGGAGAGA 11 0.27499999999999997 No Hit GCTTGTTATATGGGTCGTGAGTGGGAACTTAGTTTCCGTTTGGGTATGCG 10 0.25 No Hit GTGGGAACTTAGTTTCCGTTTGGGTATGCGCCCTTGGATTGCTGTTGCAT 10 0.25 No Hit AGTGACCAGGCCCGGGCGACTGTTTACCAAAAACACAGGTCTCCGCAAAG 10 0.25 No Hit CCTGCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGCAA 9 0.22499999999999998 No Hit GAAACAATGACGGTATCTGAGGAATAAGCATCGGCTAACTCTGTGCCAGC 9 0.22499999999999998 No Hit GCTTAACACATGCAAGTCGAACGGGAAGTGGTGTTTCCAGTGGCGAACGG 9 0.22499999999999998 No Hit CCTGGCTCAGGATGAACGCTGGCGGCATGCTTAACACATGCAAGTCGAAC 8 0.2 No Hit GCGAAAGCCTGACGGAGCAATGCCGCGTGGAGGTGGAAGGCCTACGGGTC 8 0.2 No Hit CATGCCGCGAATCCTCTTGAAAGAGAGGGGTGCCCTCGGGAACGCGGACA 8 0.2 No Hit GAACGCTGGCGGCATGCTTAACACATGCAAGTCGAACGGGAAGTGGTGTT 8 0.2 No Hit ATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAA 8 0.2 No Hit GGAAGGCCTACGGGTCGTCAACTTCTTTTCTCGGAGAAGAAACAATGACG 8 0.2 No Hit GGAAGCTTCTCTGATGGTATGCCTTTAGGAATATCTGGTACTTTCAACTT 8 0.2 No Hit CTTACCAGGTCCAGACATAGCAAGGATTGACAGACTGAGAGCTCTTTCTT 8 0.2 No Hit CAGGGCTTGACATGCCGCGAATCCTCTTGAAAGAGAGGGGTGCCCTCGGG 7 0.17500000000000002 No Hit GTTGCTAATACCCCGTAGGCTGAGGAGCAAAAGGAGAAATCCGCCCAAGG 7 0.17500000000000002 No Hit GTAAGAACCTGCCCTTGGGAGGGGAACAACAACTGGAAACGGTTGCTAAT 7 0.17500000000000002 No Hit CAACAACTGGAAACGGTTGCTAATACCCCGTAGGCTGAGGAGCAAAAGGA 7 0.17500000000000002 No Hit CTTGCGGTGGATACCTAGGCACCCAGAGACGAGGAAGGGCGTAGCAAGCG 7 0.17500000000000002 No Hit GATGAACGCTGGCGGCATGCTTAACACATGCAAGTCGAACGGGAAGTGGT 7 0.17500000000000002 No Hit GTGACCAGGCCCGGGCGACTGTTTACCAAAAACACAGGTCTCCGCAAAGT 7 0.17500000000000002 No Hit CTATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAA 7 0.17500000000000002 No Hit TGAACGCTGGCGGCATGCTTAACACATGCAAGTCGAACGGGAAGTGGTGT 7 0.17500000000000002 No Hit GGGTAAGAAGGGGTAGAGAAAATGCCTCGAGCCGAGGTCCGAGTACCAAG 7 0.17500000000000002 No Hit GGAAGAGACTTATAATATTGTGGCTGCTCATGGTTATTTTGGCCGATTAA 6 0.15 No Hit CGGGAAGTGGTGTTTCCAGTGGCGAACGGGTGAGTAACGCGTAAGAACCT 6 0.15 No Hit GTTAAGTCTCGCAACGAGCGCAACCCTCGTGTTTAGTTGCCACTATGAGT 6 0.15 No Hit GGATGAACGCTGGCGGCATGCTTAACACATGCAAGTCGAACGGGAAGTGG 6 0.15 No Hit GGAGTCTGACATGCGTGCGAGTCGACGGGTTCTGAAACCTGGGATGCGCA 6 0.15 No Hit GTGGAATCCCGTGTGAATCAGCAAGGACCACCTTGCAAGGCTAAATACTC 6 0.15 No Hit GCACAACATCCTTATGCATCCATTTCACATGTTAGGTGTAGCTGGTGTTT 6 0.15 No Hit GAGTTCGATCCTGGCTCAGGATGAACGCTGGCGGCATGCTTAACACATGC 6 0.15 No Hit GGCAGAGGGAATTTCCGGTGGAGCGGTGAAATGCATTGAGATCGGAAAGA 6 0.15 No Hit TATCTATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTT 6 0.15 No Hit AGAGTTCGATCCTGGCTCAGGATGAACGCTGGCGGCATGCTTAACACATG 6 0.15 No Hit GCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGCAAGTA 6 0.15 No Hit GGCTGTCGTCAGCTCGTGCCGTAAGGTGTTGGGTTAAGTCTCGCAACGAG 5 0.125 No Hit GGGAAACAGCCCGGATCACCAGCTAAGGCCCCTAAATGACCGCTCAGTGA 5 0.125 No Hit GTTGCCACTATGAGTTTGGAACCCTGAACAGACCGCCGGTGTTAAGCCGG 5 0.125 No Hit GTCTGGTGCCAGCAGCCGCGGTAATTCCAGCTCCAATAGCGTATATTTAA 5 0.125 No Hit GCGCCCTTGGATTGCTGTTGCATATTCAGCTCCTGTTGCAGCTGCGACTG 5 0.125 No Hit CACTGACACTGAGAGACGAAAGCTAGGGGAGCAAATGGGATTAGAGACCC 5 0.125 No Hit GCCGAAAGCATCACTAGCTTACGCTCTGACCCGAGTAGCATGGGGCACGT 5 0.125 No Hit AAGCACTGTTTCGGTGCGGGCTGCGCGAGCGGTACCAAATCGAGGCAAAC 5 0.125 No Hit GGCCTACGGGTCGTCAACTTCTTTTCTCGGAGAAGAAACAATGACGGTAT 5 0.125 No Hit CTTGAAAGAGAGGGGTGCCCTCGGGAACGCGGACACAGGTGGTGCATGGC 5 0.125 No Hit GCCTGACGGAGCAATGCCGCGTGGAGGTGGAAGGCCTACGGGTCGTCAAC 5 0.125 No Hit GCTTGGGAGTCCTTGCAATTTGAATAAACCAAGATCTTACCATGACTGCA 5 0.125 No Hit GGGAAACTACTGAAAATGAATCTGCTAATGAGGGTTACAAATTTGGTCAA 5 0.125 No Hit CTTATGAGCTAATTGTTCTACACTTCTTACTTGGTGTAGCTTGTTATATG 5 0.125 No Hit GGAAAGAACACCAACGGCGAAAGCACTCTGCTGGGCCGACACTGACACTG 5 0.125 No Hit GTTGAAGAATGAGCCGGCGACTCATAGGCAGTGGCTTGGTTAAGGGAACG 5 0.125 No Hit GGTGTTTCCAGTGGCGAACGGGTGAGTAACGCGTAAGAACCTGCCCTTGG 5 0.125 No Hit GGGAATTTCCGGTGGAGCGGTGAAATGCATTGAGATCGGAAAGAACACCA 5 0.125 No Hit GAGGAGCAAAAGGAGAAATCCGCCCAAGGAGGGGCTCGCGTCTGATTAGC 5 0.125 No Hit GCTAGTTTCAACAACTCTCGTTCTTTACACTTCTTCTTGGCTGCTTGGCC 5 0.125 No Hit GGTAGCGAAATTCCTTGTCGGGTAAGTTCCGACCCGCACGAAAGGCGTAA 5 0.125 No Hit GAGAAATCCGCCCAAGGAGGGGCTCGCGTCTGATTAGCTAGTTGGTGAGG 5 0.125 No Hit GCTGTTTTCTTGATTTACCCTATTGGTCAAGGAAGCTTCTCTGATGGTAT 5 0.125 No Hit GTCTGACATGCGTGCGAGTCGACGGGTTCTGAAACCTGGGATGCGCAAGG 5 0.125 No Hit GGAATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCTAAA 5 0.125 No Hit GTGAAAACGGGGTTGTGGGAGAGCAATACAAGCGTTGTGCTGCTAGGCGA 5 0.125 No Hit GTTTGTTTGATGGTACGTGCTACTCGGATAACCGTAGTAATTCTAGAGCT 5 0.125 No Hit GGTAGGGGCAGAGGGAATTTCCGGTGGAGCGGTGAAATGCATTGAGATCG 5 0.125 No Hit GCCTAAACCGTGAAAACGGGGTTGTGGGAGAGCAATACAAGCGTTGTGCT 5 0.125 No Hit CCTAGGCACCCAGAGACGAGGAAGGGCGTAGCAAGCGACGAAATGCTTCG 5 0.125 No Hit GCAAAAGGAGAAATCCGCCCAAGGAGGGGCTCGCGTCTGATTAGCTAGTT 5 0.125 No Hit AGGGAAACTACTGAAAATGAATCTGCTAATGAGGGTTACAAATTTGGTCA 5 0.125 No Hit AGAGGATGCAAGCGTTATCCGGAATGATTGGGCGTAAAGCGTCTGTAGGT 5 0.125 No Hit GTTCAACCTAAATGGATTCAATTTCAACCAATCTGTAGTTGATAGTCAAG 5 0.125 No Hit AAGGCTTGCGGTGGATACCTAGGCACCCAGAGACGAGGAAGGGCGTAGCA 5 0.125 No Hit ATCTATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTG 5 0.125 No Hit GAGCAAATGGGATTAGAGACCCCAGTAGTCCTAGCCGTAAACGATGGATA 5 0.125 No Hit GCTGGTTCTCCCCGAAATGCGTTGAGGCGCAGCAGTTGACTGGACATCTA 5 0.125 No Hit GCTAACTCCAAAAACCCGTCCTCAGTTCGGATTGCAGGCTGCAACTCGCC 5 0.125 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0125 0.0 0.0 0.0 0.0 48-49 0.025 0.0 0.0 0.0 0.0 50-51 0.037500000000000006 0.0 0.0 0.0 0.0 52-53 0.05 0.0 0.0 0.0 0.0 54-55 0.05 0.0 0.0 0.0 0.0 56-57 0.05 0.0 0.0 0.0 0.0 58-59 0.05 0.0 0.0 0.0 0.0 60-61 0.0625 0.0 0.0 0.0 0.0 62-63 0.125 0.0 0.0 0.0 0.0 64-65 0.175 0.0 0.0 0.0 0.0 66-67 0.21250000000000002 0.0 0.0 0.0 0.0 68-69 0.30000000000000004 0.0 0.0 0.0 0.0 70-71 0.3625 0.0 0.0 0.0 0.0 72-73 0.4125 0.0 0.0 0.0 0.0 74-75 0.48750000000000004 0.0 0.0 0.0 0.0 76-77 0.6125 0.0 0.0 0.0 0.0 78-79 0.7124999999999999 0.0 0.0 0.0 0.0 80-81 0.7375 0.0 0.0 0.0 0.0 82-83 0.85 0.0 0.0 0.0 0.0 84-85 0.95 0.0 0.0 0.0 0.0 86-87 1.1875 0.0 0.0 0.0 0.0 88-89 1.4375 0.0 0.0 0.0 0.0 90-91 1.7875 0.0 0.0 0.0 0.0 92-93 2.0125 0.0 0.0 0.0 0.0 94-95 2.2625 0.0 0.0 0.0 0.0 96-97 2.45 0.0 0.0 0.0 0.0 98-99 2.9000000000000004 0.0 0.0 0.0 0.0 100-101 3.3125 0.0 0.0 0.0 0.0 102-103 3.6500000000000004 0.0 0.0 0.0 0.0 104-105 4.15 0.0 0.0 0.0 0.0 106-107 4.6625 0.0 0.0 0.0 0.0 108-109 4.775 0.0 0.0 0.0 0.0 110-111 4.775 0.0 0.0 0.0 0.0 112-113 4.775 0.0 0.0 0.0 0.0 114-115 4.775 0.0 0.0 0.0 0.0 116-117 4.775 0.0 0.0 0.0 0.0 118-119 4.775 0.0 0.0 0.0 0.0 120-121 4.775 0.0 0.0 0.0 0.0 122-123 4.775 0.0 0.0 0.0 0.0 124-125 4.775 0.0 0.0 0.0 0.0 126-127 4.775 0.0 0.0 0.0 0.0 128-129 4.775 0.0 0.0 0.0 0.0 130-131 4.775 0.0 0.0 0.0 0.0 132-133 4.775 0.0 0.0 0.0 0.0 134-135 4.775 0.0 0.0 0.0 0.0 136-137 4.775 0.0 0.0 0.0 0.0 138-139 4.775 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content pass >>END_MODULE Read 1127669 spots for SRR6941534.sra Written 1127669 spots for SRR6941534.sra Read 1127669 spots for SRR6941534.sra Written 1127669 spots for SRR6941534.sra Read 1127669 spots for SRR6941534.sra Written 1127669 spots for SRR6941534.sra Read 1127669 spots for SRR6941534.sra Written 1127669 spots for SRR6941534.sra Read 1127669 spots for SRR6941534.sra Written 1127669 spots for SRR6941534.sra Read 1127669 spots for SRR6941534.sra Written 1127669 spots for SRR6941534.sra Read 1127669 spots for SRR6941534.sra Written 1127669 spots for SRR6941534.sra Read 1127669 spots for SRR6941534.sra Written 1127669 spots for SRR6941534.sra Read 1127680 spots for SRR6941534.sra Written 1127680 spots for SRR6941534.sra Read 1127669 spots for SRR6941534.sra Written 1127669 spots for SRR6941534.sra Read 1127669 spots for SRR6941534.sra Written 1127669 spots for SRR6941534.sra Read 1127669 spots for SRR6941534.sra Written 1127669 spots for SRR6941534.sra Read 1127669 spots for SRR6941534.sra Written 1127669 spots for SRR6941534.sra Read 1127669 spots for SRR6941534.sra Written 1127669 spots for SRR6941534.sra Read 1127669 spots for SRR6941534.sra Written 1127669 spots for SRR6941534.sra Read 1127669 spots for SRR6941534.sra Written 1127669 spots for SRR6941534.sra Read 1127669 spots for SRR6941534.sra Written 1127669 spots for SRR6941534.sra Read 1127669 spots for SRR6941534.sra Written 1127669 spots for SRR6941534.sra Read 1127669 spots for SRR6941534.sra Written 1127669 spots for SRR6941534.sra Read 1127669 spots for SRR6941534.sra Written 1127669 spots for SRR6941534.sra SRR ids: ['SRR6941534.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_ri6zg6s4 SRR6941534.sra spots: 22553391 blocks: [[1, 1127669], [1127670, 2255338], [2255339, 3383007], [3383008, 4510676], [4510677, 5638345], [5638346, 6766014], [6766015, 7893683], [7893684, 9021352], [9021353, 10149021], [10149022, 11276690], [11276691, 12404359], [12404360, 13532028], [13532029, 14659697], [14659698, 15787366], [15787367, 16915035], [16915036, 18042704], [18042705, 19170373], [19170374, 20298042], [20298043, 21425711], [21425712, 22553391]] SRR6941534 file size 7620903 SRR6941534 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR6941534 SRR6941534_1.fastq SRR6941534_2.fastq Input file: SRR6941534_1.fastq Paired file: SRR6941534_2.fastq trimmed: SRR6941534-trimmed-pair1.fastq, SRR6941534-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Fri Dec 6 10:22:35 2024 >> started Fri Dec 6 10:23:04 2024 >> done (28.483s) 22553391 read pairs processed; of these: 24378 ( 0.11%) short read pairs filtered out after trimming by size control 21061 ( 0.09%) empty read pairs filtered out after trimming by size control 22507952 (99.80%) read pairs available; of these: 18955443 (84.22%) trimmed read pairs available after processing 3552509 (15.78%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 2 0.00% 19 7 0.00% 20 4 0.00% 21 7 0.00% 22 5 0.00% 23 4 0.00% 24 11 0.00% 25 14 0.00% 26 13 0.00% 27 19 0.00% 28 31 0.00% 29 38 0.00% 30 64 0.00% 31 63 0.00% 32 75 0.00% 33 58 0.00% 34 85 0.00% 35 95 0.00% 36 84 0.00% 37 127 0.00% 38 136 0.00% 39 184 0.00% 40 182 0.00% 41 226 0.00% 42 248 0.00% 43 276 0.00% 44 322 0.00% 45 363 0.00% 46 379 0.00% 47 402 0.00% 48 453 0.00% 49 570 0.00% 50 664 0.00% 51 781 0.00% 52 903 0.00% 53 1003 0.00% 54 1165 0.01% 55 1231 0.01% 56 1320 0.01% 57 1500 0.01% 58 1805 0.01% 59 1942 0.01% 60 2198 0.01% 61 3101 0.01% 62 3512 0.02% 63 3564 0.02% 64 4098 0.02% 65 4480 0.02% 66 4800 0.02% 67 5117 0.02% 68 6092 0.03% 69 7326 0.03% 70 8217 0.04% 71 8698 0.04% 72 10610 0.05% 73 11590 0.05% 74 11413 0.05% 75 12584 0.06% 76 13672 0.06% 77 16419 0.07% 78 15202 0.07% 79 18473 0.08% 80 19917 0.09% 81 20889 0.09% 82 22367 0.10% 83 24886 0.11% 84 26568 0.12% 85 32151 0.14% 86 33223 0.15% 87 34248 0.15% 88 38515 0.17% 89 36473 0.16% 90 37905 0.17% 91 40422 0.18% 92 42918 0.19% 93 48236 0.21% 94 52312 0.23% 95 46843 0.21% 96 46396 0.21% 97 50423 0.22% 98 47355 0.21% 99 49444 0.22% 100 50223 0.22% 101 54882 0.24% 102 58342 0.26% 103 59315 0.26% 104 62835 0.28% 105 61012 0.27% 106 60625 0.27% 107 62083 0.28% 108 64701 0.29% 109 80227 0.36% 110 67368 0.30% 111 75184 0.33% 112 91301 0.41% 113 66099 0.29% 114 80278 0.36% 115 76551 0.34% 116 96800 0.43% 117 98187 0.44% 118 94550 0.42% 119 110864 0.49% 120 120583 0.54% 121 119161 0.53% 122 119593 0.53% 123 132305 0.59% 124 133359 0.59% 125 142086 0.63% 126 143276 0.64% 127 153743 0.68% 128 174909 0.78% 129 240811 1.07% 130 435978 1.94% 131 873640 3.88% 132 657520 2.92% 133 3304322 14.68% 134 4142387 18.40% 135 78357 0.35% 136 63909 0.28% 137 66140 0.29% 138 76573 0.34% 139 109177 0.49% 140 497744 2.21% 141 1291307 5.74% 142 207800 0.92% 143 1615972 7.18% 144 108746 0.48% 145 489557 2.18% 146 39882 0.18% 147 47137 0.21% 148 60263 0.27% 149 96948 0.43% 150 801313 3.56% 151 3552509 15.78% 22507952 reads passed initial QC criterion=sequence-density sequence-density=1.02 sequence-density-rank=1 fanout-score=5.38 fanout-score-rank=17 prefix-density=3.06 prefix-fanout=1.8 sequence=ATTTAGCCTTGGACGGAGTCTACCGCCCGATTTGGGCTGCATTCCCAAACAACCCGACTCGTTGACGGCGCCTCGTGGGGCGACAGGGTCCGGGCCGGACGGGGCTCTCACCCTCCCAGGCGCCCCTTTCCAGGGGACTTGGGCCCGGTCCGTCGCTGAGGACGCCTCTCCAGACTACAATTCGGACGGCACGGCCGCCCGATTCTCAAGCTGGGCTGCTCCCGGTTCGCTCGCCGTTACTAGGGGAATCCTTGTAAGTTTCTTCTCCTCCGCTTATTTATATGCTTAAACTCAGCGGGTAGTCCCGCCTGACCTGG criterion=fanout-score sequence-density=0.02 sequence-density-rank=35 fanout-score=49.82 fanout-score-rank=1 prefix-density=0.84 prefix-fanout=1.0 sequence=GCATCGCCGGCCCCCATCCGCTTCCCTCCCGGCAATTTCAAGCACTCTTTGACTCTCTTTTCAAAGTCCTTTTCATCTTTCCCTCGCGGTACTTGTTCGCTATCGG criterion=sequence-density sequence-density=0.71 sequence-density-rank=1 fanout-score=2.09 fanout-score-rank=26 prefix-density=0.72 prefix-fanout=2.1 sequence=CCTAGTACGAGAGGA criterion=fanout-score sequence-density=0.01 sequence-density-rank=30 fanout-score=109.88 fanout-score-rank=1 prefix-density=1.49 prefix-fanout=1.1 sequence=AGAAGGGGTGCCCCCTCACAAAAGGGGGTCGCAGTGACCAGGCCCGGGCGACTGTTTACCAAAAACACAGGTCTCCGCAAAGTCGTAAGACCATGTATGGGGGCTGACGCCTGCCCAGTGCCGGAAGGTCAAGGAAGTTGGTGAACTGATGACAGGGAAGCCGGCGACCGAAGCCCCGGTGAACGGCGGCCGTAAC SRR6941534 testing PE reads STAR mapping to Ensembl genome Started job on | Dec 06 10:23:51 Started mapping on | Dec 06 10:23:51 Finished on | Dec 06 10:25:32 Mapping speed, Million of reads per hour | 802.26 Number of input reads | 22507952 Average input read length | 269 UNIQUE READS: Uniquely mapped reads number | 8694959 Uniquely mapped reads % | 38.63% Average mapped length | 273.11 Number of splices: Total | 1197198 Number of splices: Annotated (sjdb) | 860237 Number of splices: GT/AG | 928272 Number of splices: GC/AG | 14963 Number of splices: AT/AC | 5409 Number of splices: Non-canonical | 248554 Mismatch rate per base, % | 0.31% Deletion rate per base | 0.01% Deletion average length | 1.51 Insertion rate per base | 0.00% Insertion average length | 1.89 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 8919734 % of reads mapped to multiple loci | 39.63% Number of reads mapped to too many loci | 513617 % of reads mapped to too many loci | 2.28% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 7.03% % of reads unmapped: other | 12.43% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 4905826 4905826 4905826 N_multimapping 8919734 8919734 8919734 N_noFeature 6009601 8542370 6075802 N_ambiguous 214192 3783 129835 UnstrandedReadsAssigned:2471166 PositiveStrandReadsAssigned:148806 NegativeStrandReadsAssigned:2489322 Dataset is classified negative stranded MeadianReadLen=151 20thPercentileLength=148 echo kmer=143 SRR6941534 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,972 [index] number of k-mers: 66,720,672 [index] number of equivalence classes: 111,837 [quant] running in paired-end mode [quant] will process pair 1: SRR6941534-trimmed-pair1.fastq SRR6941534-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 22,507,952 reads, 6,553,955 reads pseudoaligned [quant] estimated average fragment length: 199.604 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 955 rounds 52973 SRR6941534.ke.tsv 35125 SRR6941534.se.tsv 88098 total ==> SRR6941534.ke.tsv <== target_id length eff_length est_counts tpm PNS24245 936 737.637 2.19897 0.187821 PNS24247 1044 845.396 0 0 PNS24249 1928 1729.4 7.83153 0.285311 PNS24246 1044 845.396 0 0 PNS24248 1044 845.396 0 0 PNS24244 1471 1272.4 5.9695 0.295585 PNS24243 293 114.978 0 0 KQK14069 1603 1404.4 534.448 23.9763 KQK14071 474 279.098 27.4654 6.20007 ==> SRR6941534.se.tsv <== BRADI_1g14170v3 676 BRADI_1g53295v3 4 BRADI_1g59795v3 11 BRADI_1g07683v3 0 BRADI_1g00485v3 0 BRADI_1g20270v3 10 BRADI_1g74790v3 4 BRADI_1g09890v3 0 BRADI_1g77505v3 5 BRADI_1g48960v3 0 SRR6941534 completed mapping pipeline successfully