Starting /dee2/code/volunteer_pipeline.sh SRR6941541 current disk space = 1551502503936 free memory = 1600838676 SRR6941541 SRAfilesize b21a6e1206f713670be95e06b59310c6 SRR6941541.sra SRR6941541.sra file validated SRR6941541 is paired end SRR6941541 is conventional basespace SRR6941541 read1 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR6941541_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 52 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 34.5455 35.0 35.0 35.0 34.0 35.0 2 34.6015 35.0 35.0 35.0 35.0 35.0 3 34.679 35.0 35.0 35.0 35.0 35.0 4 34.71275 35.0 35.0 35.0 35.0 35.0 5 34.55575 35.0 35.0 35.0 34.0 35.0 6 39.4295 40.0 40.0 40.0 39.0 40.0 7 39.3735 40.0 40.0 40.0 39.0 40.0 8 39.297 40.0 40.0 40.0 39.0 40.0 9 39.43875 40.0 40.0 40.0 39.0 40.0 10-14 39.38645 40.0 40.0 40.0 39.0 40.0 15-19 39.21815 40.0 40.0 40.0 38.8 40.0 20-24 39.3483 40.0 40.0 40.0 39.0 40.0 25-29 39.3266 40.0 40.0 40.0 39.0 40.0 30-34 39.260000000000005 40.0 40.0 40.0 38.8 40.0 35-39 39.3326 40.0 40.0 40.0 39.0 40.0 40-44 39.3238 40.0 40.0 40.0 39.0 40.0 45-49 39.23695 40.0 40.0 40.0 38.8 40.0 50-54 39.22365 40.0 40.0 40.0 38.8 40.0 55-59 39.205850000000005 40.0 40.0 40.0 38.8 40.0 60-64 39.1656 40.0 40.0 40.0 38.8 40.0 65-69 39.1695 40.0 40.0 40.0 38.8 40.0 70-74 39.147149999999996 40.0 40.0 40.0 38.4 40.0 75-79 39.0199 40.0 39.8 40.0 37.8 40.0 80-84 39.08785 40.0 40.0 40.0 38.0 40.0 85-89 38.92085000000001 40.0 39.4 40.0 37.4 40.0 90-94 39.013549999999995 40.0 39.2 40.0 37.8 40.0 95-99 38.8513 40.0 39.0 40.0 37.0 40.0 100-104 38.25785 39.4 38.4 39.8 36.2 39.8 105-109 38.9168 40.0 39.0 40.0 37.2 40.0 110-114 38.8497 40.0 39.0 40.0 36.8 40.0 115-119 38.8512 40.0 39.0 40.0 37.0 40.0 120-124 38.6919 40.0 39.0 40.0 36.4 40.0 125-129 38.523300000000006 40.0 39.0 40.0 36.0 40.0 130-134 38.433749999999996 40.0 39.0 40.0 35.8 40.0 135-139 38.344649999999994 40.0 39.0 40.0 35.8 40.0 140-144 38.12585 40.0 39.0 40.0 35.4 40.0 145-149 37.5642 40.0 39.0 40.0 34.0 40.0 150-151 34.5835 38.0 35.0 39.5 18.0 40.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 12 1.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 1.0 20 1.0 21 0.0 22 1.0 23 1.0 24 5.0 25 0.0 26 6.0 27 14.0 28 15.0 29 24.0 30 20.0 31 28.0 32 50.0 33 39.0 34 68.0 35 91.0 36 115.0 37 181.0 38 338.0 39 3001.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.392392392392395 6.831831831831832 4.1041041041041035 46.671671671671675 2 18.893893893893893 8.458458458458457 32.78278278278278 39.86486486486486 3 16.725 10.575 25.624999999999996 47.075 4 24.425 18.9 20.45 36.225 5 24.79318124843319 22.98821759839559 24.968663825520178 27.24993732765104 6 24.05 24.025 25.074999999999996 26.85 7 15.4 23.7 42.725 18.175 8 20.424999999999997 19.0 34.0 26.575 9 21.45 18.55 33.650000000000006 26.35 10-14 21.435000000000002 23.025000000000002 27.589999999999996 27.950000000000003 15-19 23.155 22.134999999999998 25.695 29.015 20-24 22.915 23.365 25.88 27.839999999999996 25-29 23.535 21.195 27.500000000000004 27.77 30-34 23.345 21.27 25.130000000000003 30.255 35-39 22.785 21.595 26.695 28.925 40-44 23.52 22.455 25.174999999999997 28.849999999999998 45-49 24.14 20.275000000000002 25.805 29.78 50-54 23.425 21.145 26.47 28.96 55-59 21.955 22.439999999999998 27.145000000000003 28.46 60-64 25.155 21.265 26.205000000000002 27.375 65-69 23.585 24.3 24.65 27.465 70-74 23.385 22.915 24.935 28.765 75-79 23.49 23.43 25.355 27.725 80-84 22.395 24.48 24.21 28.915000000000003 85-89 23.84 23.474999999999998 24.145 28.54 90-94 24.555 23.330000000000002 23.87 28.244999999999997 95-99 24.075 23.345 24.785 27.794999999999998 100-104 23.535 22.85 25.365 28.249999999999996 105-109 22.175 23.07 25.11 29.645 110-114 22.54 23.035 26.150000000000002 28.275 115-119 22.49 23.28 25.64 28.59 120-124 23.385 25.495 23.21 27.91 125-129 22.38 24.115000000000002 24.33 29.175 130-134 24.345 21.935 23.685000000000002 30.035 135-139 21.834999999999997 23.21 25.405 29.549999999999997 140-144 23.845 23.415 24.245 28.494999999999997 145-149 22.07 24.325 25.03 28.575 150-151 21.719004128612536 23.94595270862004 24.94682847491555 29.38821468785187 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.5 22 1.5 23 2.5 24 2.0 25 0.5 26 0.5 27 1.0 28 1.0 29 4.0 30 7.0 31 6.5 32 9.0 33 10.0 34 8.5 35 15.0 36 31.0 37 43.5 38 57.0 39 78.5 40 76.0 41 84.0 42 89.0 43 71.5 44 72.0 45 87.5 46 97.5 47 79.0 48 63.5 49 90.5 50 119.5 51 141.5 52 149.0 53 178.5 54 245.0 55 383.0 56 422.0 57 286.0 58 233.0 59 229.0 60 170.0 61 96.5 62 60.5 63 53.5 64 35.0 65 17.0 66 13.0 67 8.0 68 10.5 69 8.0 70 5.0 71 8.0 72 11.5 73 10.5 74 5.0 75 4.5 76 5.0 77 1.5 78 0.5 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1 2 0.1 3 0.0 4 0.0 5 0.27499999999999997 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.08750000000000001 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.35 #Duplication Level Percentage of deduplicated Percentage of total 1 73.26106594399278 40.550000000000004 2 11.969286359530262 13.25 3 6.052393857271906 10.05 4 3.342366757000903 7.3999999999999995 5 1.5808491418247517 4.375 6 0.8581752484191508 2.85 7 0.5871725383920506 2.275 8 0.49683830171635046 2.1999999999999997 9 0.36133694670280037 1.7999999999999998 >10 1.4001806684733513 12.049999999999999 >50 0.09033423667570009 3.2 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GGCATGATGACTTGGCCTCATCCTCTCCTTCCTCCGGCTTAACACCGGCG 72 1.7999999999999998 No Hit CCCCACTGCTGCCTCCCGTAGGAGTCTGGGCCGTGTCTCAGTCCCAGTGT 56 1.4000000000000001 No Hit CCCGTCAATTCCTTTGAGTTTCATTCTTGCGAACGTACTCCCCAGGCGGG 38 0.95 No Hit GTGCTCTGCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTCCTA 35 0.8750000000000001 No Hit GTCCAGTCAACTGCTGCGCCTCAACGCATTTCGGGGAGAACCAGCTAGCT 29 0.7250000000000001 No Hit CCCACTGCTGCCTCCCGTAGGAGTCTGGGCCGTGTCTCAGTCCCAGTGTG 27 0.675 No Hit CCGGGAACGGATTCACCGCCGTATGGCTGACCGGCGATTACTAGCGATTC 20 0.5 No Hit GGGGCATGATGACTTGGCCTCATCCTCTCCTTCCTCCGGCTTAACACCGG 18 0.44999999999999996 No Hit CCTAGCTTTCGTCTCTCAGTGTCAGTGTCGGCCCAGCAGAGTGCTTTCGC 17 0.42500000000000004 No Hit CCTCGCGGTACTTGTTCGCTATCGGTCTCTCGCCTGTATTTAGCCTTGGA 16 0.4 No Hit CTCCACGCGGCATTGCTCCGTCAGGCTTTCGCCCATTGCGGAAAATTCCC 16 0.4 No Hit GTCGGTTTCGGGTACAGGTACCCTTTTGTTGAAGGTCGTTCGAGCTTTTC 15 0.375 No Hit GTTCGAGCTTTTCCTGGGAGTATGGCATCGGTTACATACTTCAGTGCCGT 14 0.35000000000000003 No Hit GCCACCTACAGACGCTTTACGCCCAATCATTCCGGATAACGCTTGCATCC 14 0.35000000000000003 No Hit GTCCCTATTAATCATTACTCCGATCCCGAAGGCCAACACAATAGGACCGG 14 0.35000000000000003 No Hit GCTCCTCAGCCTACGGGGTATTAGCAACCGTTTCCAGTTGTTGTTCCCCT 14 0.35000000000000003 No Hit CCTAGATGTCCAGTCAACTGCTGCGCCTCAACGCATTTCGGGGAGAACCA 13 0.325 No Hit GGCGATAATAAATACAGAAGTTGCGGTCAATAAGGTAGGGATCATCAAAA 13 0.325 No Hit CCCACCTGTGTCGGTTTCGGGTACAGGTACCCTTTTGTTGAAGGTCGTTC 13 0.325 No Hit GACCTGTTGTCCATCGACTACGCCTTTCGGCCTGATCTTAGGCCCTGACT 13 0.325 No Hit CCCTACCGTACTCCAGCTTGGTAGTTTCCACCGCCTGTCCAGGGTTGAGC 13 0.325 No Hit CCGTCAATTCCTTTGAGTTTCATTCTTGCGAACGTACTCCCCAGGCGGGA 12 0.3 No Hit GTGTCCTTAAACCTATAACCATCTTTCGGCTAACCTAGCCTCCTCCGTCC 12 0.3 No Hit CTCTGCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTCCTAAAG 12 0.3 No Hit CTCCAGACTACAATTCGGACGGCACGGCCGCCCGATTCTCAAGCTGGGCT 11 0.27499999999999997 No Hit CTCCTTTATCACTGAGCGGTCATTTAGGGGCCTTAGCTGGTGATCCGGGC 11 0.27499999999999997 No Hit GCTCCCCTAGCTTTCGTCTCTCAGTGTCAGTGTCGGCCCAGCAGAGTGCT 11 0.27499999999999997 No Hit CTTTCTTTTCCTCTGGCTACTAAGATGTTTCAGTTCGCCAGGTTGTCTCT 11 0.27499999999999997 No Hit CCTTAAACCTATAACCATCTTTCGGCTAACCTAGCCTCCTCCGTCCCTCC 10 0.25 No Hit GCCCAATCATTCCGGATAACGCTTGCATCCTCTGTCTTACCGCGGCTGCT 10 0.25 No Hit CCGTCATTGTTTCTTCTCCGAGAAAAGAAGTTGACGACCCGTAGGCCTTC 10 0.25 No Hit GGTCGTTCGAGCTTTTCCTGGGAGTATGGCATCGGTTACATACTTCAGTG 10 0.25 No Hit CCCTAGAGTAACTTTTATCCGTTGAGCGACGGCCCTTCCACTCGGCACCG 10 0.25 No Hit CCACTGCTGCCTCCCGTAGGAGTCTGGGCCGTGTCTCAGTCCCAGTGTGG 9 0.22499999999999998 No Hit GTGTCGGTTTCGGGTACAGGTACCCTTTTGTTGAAGGTCGTTCGAGCTTT 9 0.22499999999999998 No Hit CCTCACGGTACTACTTCGCTATCGGTCACCCAGGAGTATTTAGCCTTGCA 9 0.22499999999999998 No Hit GTCGGCATCGTTTATGGTTGAGACTAGGACGGTATCTGATCGTCTTCGAG 9 0.22499999999999998 No Hit CTCCTTTTGCTCCTCAGCCTACGGGGTATTAGCAACCGTTTCCAGTTGTT 9 0.22499999999999998 No Hit CCCATCACGATGAAATTTCCCAAGATTACCCGGGCCTGTCGGCCAAGGCT 9 0.22499999999999998 No Hit GCTTTCTTTTCCTCTGGCTACTAAGATGTTTCAGTTCGCCAGGTTGTCTC 9 0.22499999999999998 No Hit CTCTGCCCCTACCGTACTCCAGCTTGGTAGTTTCCACCGCCTGTCCAGGG 9 0.22499999999999998 No Hit GTTCTATTTCACTACCCACTGGGGGTTCTTTTCACCTTTCCCTCACGGTA 8 0.2 No Hit GTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTC 8 0.2 No Hit CCTCAGCCTACGGGGTATTAGCAACCGTTTCCAGTTGTTGTTCCCCTCCC 8 0.2 No Hit CCTGTATTTAGCCTTGGACGGAGTCTACCGCCCGATTTGGGCTGCATTCC 8 0.2 No Hit CTCACGTACCGCATTAATGGGCGAACAGCCCAACCCTTGGAACCACCTAC 8 0.2 No Hit CCCGGGAACGGATTCACCGCCGTATGGCTGACCGGCGATTACTAGCGATT 8 0.2 No Hit GTGAGCATTACGTTCGTGCATTACTTCCATACCAAGATTAGCACGGTTGA 8 0.2 No Hit GCATGATGACTTGGCCTCATCCTCTCCTTCCTCCGGCTTAACACCGGCGG 8 0.2 No Hit GTCCCAGTGTGGCTGATCATCCTCTCGGACCAGCTACTGATCATCGCCTT 8 0.2 No Hit AGCACGTGTGTCGCCCAGGGCATAAGGGGCATGATGACTTGGCCTCATCC 8 0.2 No Hit GTCGGTTCGGACCTCTGCTTAGTTTCATCCAAGCTTCATCCTGGTCATGG 8 0.2 No Hit CTTGTGTCCTTAAACCTATAACCATCTTTCGGCTAACCTAGCCTCCTCCG 7 0.17500000000000002 No Hit CTCCTCAGCCTACGGGGTATTAGCAACCGTTTCCAGTTGTTGTTCCCCTC 7 0.17500000000000002 No Hit GCTCCGTCAGGCTTTCGCCCATTGCGGAAAATTCCCCACTGCTGCCTCCC 7 0.17500000000000002 No Hit CTCCCATTTCGCTCGCCGCTACTACGGGAATCGCTTTTGCTTTCTTTTCC 7 0.17500000000000002 No Hit CGGGAACGGATTCACCGCCGTATGGCTGACCGGCGATTACTAGCGATTCC 7 0.17500000000000002 No Hit GCCCCATGCTACTCGGGTCAGAGCGTAAGCTAGTGATGCTTTCGGCTACT 7 0.17500000000000002 No Hit ATCGTTTACGGCTAGGACTACTGGGGTCTCTAATCCCATTTGCTCCCCTA 7 0.17500000000000002 No Hit GCGGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTACG 7 0.17500000000000002 No Hit CATCGTTTACGGCTAGGACTACTGGGGTCTCTAATCCCATTTGCTCCCCT 7 0.17500000000000002 No Hit GTTCCGTTCCCTTAACCAAGCCACTGCCTATGAGTCGCCGGCTCATTCTT 7 0.17500000000000002 No Hit CTTCGCTATCGGTCACCCAGGAGTATTTAGCCTTGCAAGGTGGTCCTTGC 7 0.17500000000000002 No Hit CCGCATTAATGGGCGAACAGCCCAACCCTTGGAACCACCTACAGCTCCAG 7 0.17500000000000002 No Hit GTCCTTAAACCTATAACCATCTTTCGGCTAACCTAGCCTCCTCCGTCCCT 7 0.17500000000000002 No Hit GTCCAGGTGCAGGTAGTCCGCATCTTCACAGACATGTCTATTTCACCGAG 6 0.15 No Hit GCCCCGTTCATCTTCAGCGCAAGGGCGCTCGATCAGTGAGCTATTACGCA 6 0.15 No Hit GGAGGGGCTGCGATGAAGGCGATAATAAATACAGAAGTTGCGGTCAATAA 6 0.15 No Hit GTCTGACACAAGGTTAGAATCCGAGCTCTTCCAGAGTGGTATCTCACTGA 6 0.15 No Hit CTTGTCCGTACCAGTTCTGAGTCGACTGTTCAGCGCTCGGGGAAAGCCCC 6 0.15 No Hit CCTCTGCCCCTACCGTACTCCAGCTTGGTAGTTTCCACCGCCTGTCCAGG 6 0.15 No Hit GTCTCTCAGTGTCAGTGTCGGCCCAGCAGAGTGCTTTCGCCGTTGGTGTT 6 0.15 No Hit GCTTGTATTGCTCTCCCACAACCCCGTTTTCACGGTTTAGGCTGCTCCCA 6 0.15 No Hit GTTCGTTAACGGAATTAACCAGACAAATCGCTCCACCAACTAAGAACGGC 6 0.15 No Hit CCTCTCCGCACTTGGCTACCCAGCGTTTACCGTAGGCACGATAACTGGTA 6 0.15 No Hit GTCGTTCGAGCTTTTCCTGGGAGTATGGCATCGGTTACATACTTCAGTGC 6 0.15 No Hit GTTAGCGGAAAAACCTTATCCATTAATAGCGGGAACTTCAAGAGCAGCTA 6 0.15 No Hit CTCAGATACCGTCATTGTTTCTTCTCCGAGAAAAGAAGTTGACGACCCGT 6 0.15 No Hit ACCTGTTGTCCATCGACTACGCCTTTCGGCCTGATCTTAGGCCCTGACTC 6 0.15 No Hit CTCTAAGGCGGAACGCTCCCCTACCGATGCATTTTGACATCCCACAGCTT 6 0.15 No Hit ATCACGATGAAATTTCCCAAGATTACCCGGGCCTGTCGGCCAAGGCTATA 6 0.15 No Hit GCCTCACCAACTAGCTAATCAGACGCGAGCCCCTCCTTGGGCGGATTTCT 6 0.15 No Hit CCCAGCTCACGTACCGCATTAATGGGCGAACAGCCCAACCCTTGGAACCA 6 0.15 No Hit GCTCTGCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTCCTAAA 6 0.15 No Hit CACCGCTTGTGCGGGCCCCCGTCAATTCCTTTGAGTTTCATTCTTGCGAA 5 0.125 No Hit CCTGGCTGTCTTTGCACCCCCACCTCCTTTATCACTGAGCGGTCATTTAG 5 0.125 No Hit CGCAGAAGTAGGAATAATAGCACCAGAGATAATATTGTTTCCATAAAGTA 5 0.125 No Hit GTGTGTACAAAGGGCAGGGACGTAGTCAACGCGAGCTGATGACTCGCGCT 5 0.125 No Hit ATCGACTACGCCTTTCGGCCTGATCTTAGGCCCTGACTCACCCTCCGTGG 5 0.125 No Hit CTCGCCTGTATTTAGCCTTGGACGGAGTCTACCGCCCGATTTGGGCTGCA 5 0.125 No Hit CGTCAATTCCTTTGAGTTTCATTCTTGCGAACGTACTCCCCAGGCGGGAT 5 0.125 No Hit GTCATTGTTTCTTCTCCGAGAAAAGAAGTTGACGACCCGTAGGCCTTCCA 5 0.125 No Hit GGCATAAGGGGCATGATGACTTGGCCTCATCCTCTCCTTCCTCCGGCTTA 5 0.125 No Hit CCCCGGAACCCAAAGACTTTGATTTCTCATAAGGTGCCGGCGGAGTCCTA 5 0.125 No Hit CCCACAACCCCGTTTTCACGGTTTAGGCTGCTCCCATTTCGCTCGCCGCT 5 0.125 No Hit GTACAAAGGGCAGGGACGTAGTCAACGCGAGCTGATGACTCGCGCTTACT 5 0.125 No Hit GTCGGGGCAGGCGGCGGGCGCAGGCGCCGCTTGCTAGCTTGGATTCTGAC 5 0.125 No Hit GACCTATTTGGGAATCTCCGGATCTATGCTTATTTTCAACTCCCCGAAGC 5 0.125 No Hit GTACCGCATTAATGGGCGAACAGCCCAACCCTTGGAACCACCTACAGCTC 5 0.125 No Hit CCAGTCAACTGCTGCGCCTCAACGCATTTCGGGGAGAACCAGCTAGCTCT 5 0.125 No Hit GGGCATGATGACTTGGCCTCATCCTCTCCTTCCTCCGGCTTAACACCGGC 5 0.125 No Hit CCTATTTGGGAATCTCCGGATCTATGCTTATTTTCAACTCCCCGAAGCAT 5 0.125 No Hit GCCCCCGTCAATTCCTTTGAGTTTCATTCTTGCGAACGTACTCCCCAGGC 5 0.125 No Hit GCTAGTTATCCAGTTGCAGAAGCGACCCCACAGGCTTGTACTTTCGCGTC 5 0.125 No Hit GTCCGTCTGGCCCGAGGAAACCTTTGCACGCCTCCGTTACCTTTTGGGAG 5 0.125 No Hit CCCCGTTCATCTTCAGCGCAAGGGCGCTCGATCAGTGAGCTATTACGCAC 5 0.125 No Hit GGCAGAAATTTGAATGATGCGTCGCCGGCACGAGGGCCGTGCGATCCGTC 5 0.125 No Hit GTCGAGTTATCATGAATCATCGGATCAGCGAGCAAAGCCCGCGTCAGCCT 5 0.125 No Hit GTCCATCGACTACGCCTTTCGGCCTGATCTTAGGCCCTGACTCACCCTCC 5 0.125 No Hit CCACATGCTCCACCGCTTGTGCGGGCCCCCGTCAATTCCTTTGAGTTTCA 5 0.125 No Hit CCTCAGATACCGTCATTGTTTCTTCTCCGAGAAAAGAAGTTGACGACCCG 5 0.125 No Hit CCTGGGAGTATGGCATCGGTTACATACTTCAGTGCCGTAGCGCCTGGTAT 5 0.125 No Hit CCCCTACCGTACTCCAGCTTGGTAGTTTCCACCGCCTGTCCAGGGTTGAG 5 0.125 No Hit GGCTCATTCTTCAACAGGCACGCGGTCAGAGATCACTTTCCCCTCCCACT 5 0.125 No Hit CCGGAACCCAAAGACTTTGATTTCTCATAAGGTGCCGGCGGAGTCCTATA 5 0.125 No Hit CACTGCTGCCTCCCGTAGGAGTCTGGGCCGTGTCTCAGTCCCAGTGTGGC 5 0.125 No Hit CCTGTGTCGGTTTCGGGTACAGGTACCCTTTTGTTGAAGGTCGTTCGAGC 5 0.125 No Hit GCTCATTCTTCAACAGGCACGCGGTCAGAGATCACTTTCCCCTCCCACTG 5 0.125 No Hit GCCTGTATTTAGCCTTGGACGGAGTCTACCGCCCGATTTGGGCTGCATTC 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.025 0.0 0.0 0.0 0.0 2 0.025 0.0 0.0 0.0 0.0 3 0.025 0.0 0.0 0.0 0.0 4 0.025 0.0 0.0 0.0 0.0 5 0.025 0.0 0.0 0.0 0.0 6 0.025 0.0 0.0 0.0 0.0 7 0.025 0.0 0.0 0.0 0.0 8 0.025 0.0 0.0 0.0 0.0 9 0.025 0.0 0.0 0.0 0.0 10-11 0.025 0.0 0.0 0.0 0.0 12-13 0.025 0.0 0.0 0.0 0.0 14-15 0.025 0.0 0.0 0.0 0.0 16-17 0.025 0.0 0.0 0.0 0.0 18-19 0.025 0.0 0.0 0.0 0.0 20-21 0.025 0.0 0.0 0.0 0.0 22-23 0.025 0.0 0.0 0.0 0.0 24-25 0.025 0.0 0.0 0.0 0.0 26-27 0.025 0.0 0.0 0.0 0.0 28-29 0.025 0.0 0.0 0.0 0.0 30-31 0.025 0.0 0.0 0.0 0.0 32-33 0.025 0.0 0.0 0.0 0.0 34-35 0.025 0.0 0.0 0.0 0.0 36-37 0.025 0.0 0.0 0.0 0.0 38-39 0.05 0.0 0.0 0.0 0.0 40-41 0.05 0.0 0.0 0.0 0.0 42-43 0.05 0.0 0.0 0.0 0.0 44-45 0.05 0.0 0.0 0.0 0.0 46-47 0.0625 0.0 0.0 0.0 0.0 48-49 0.1 0.0 0.0 0.0 0.0 50-51 0.125 0.0 0.0 0.0 0.0 52-53 0.1375 0.0 0.0 0.0 0.0 54-55 0.2 0.0 0.0 0.0 0.0 56-57 0.2375 0.0 0.0 0.0 0.0 58-59 0.3125 0.0 0.0 0.0 0.0 60-61 0.375 0.0 0.0 0.0 0.0 62-63 0.5 0.0 0.0 0.0 0.0 64-65 0.55 0.0 0.0 0.0 0.0 66-67 0.6125 0.0 0.0 0.0 0.0 68-69 0.7375 0.0 0.0 0.0 0.0 70-71 0.8 0.0 0.0 0.0 0.0 72-73 0.8875 0.0 0.0 0.0 0.0 74-75 1.125 0.0 0.0 0.0 0.0 76-77 1.375 0.0 0.0 0.0 0.0 78-79 1.6 0.0 0.0 0.0 0.0 80-81 1.8875000000000002 0.0 0.0 0.0 0.0 82-83 2.1125 0.0 0.0 0.0 0.0 84-85 2.325 0.0 0.0 0.0 0.0 86-87 2.7 0.0 0.0 0.0 0.0 88-89 3.0875000000000004 0.0 0.0 0.0 0.0 90-91 3.6375 0.0 0.0 0.0 0.0 92-93 4.0875 0.0 0.0 0.0 0.0 94-95 4.825 0.0 0.0 0.0 0.0 96-97 5.425 0.0 0.0 0.0 0.0 98-99 6.1 0.0 0.0 0.0 0.0 100-101 6.7375 0.0 0.0 0.0 0.0 102-103 7.225 0.0 0.0 0.0 0.0 104-105 7.762499999999999 0.0 0.0 0.0 0.0 106-107 8.3625 0.0 0.0 0.0 0.0 108-109 8.9875 0.0 0.0 0.0 0.0 110-111 9.5125 0.0 0.0 0.0 0.0 112-113 9.9125 0.0 0.0 0.0 0.0 114-115 10.524999999999999 0.0 0.0 0.0 0.0 116-117 11.212499999999999 0.0 0.0 0.0 0.0 118-119 11.825 0.0 0.0 0.0 0.0 120-121 12.325 0.0 0.0 0.0 0.0 122-123 12.912500000000001 0.0 0.0 0.0 0.0 124-125 13.7 0.0 0.0 0.0 0.0 126-127 14.5125 0.0 0.0 0.0 0.0 128-129 15.175 0.0 0.0 0.0 0.0 130-131 15.8 0.0 0.0 0.0 0.0 132-133 16.575000000000003 0.0 0.0 0.0 0.0 134-135 17.2625 0.0 0.0 0.0 0.0 136-137 17.825 0.0 0.0 0.0 0.0 138-139 18.4875 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGCGAAT 10 0.006830828 145.0 3 AGCTTCG 10 0.006830828 145.0 6 CCATCTT 10 0.006830828 145.0 3 CCACAGC 10 0.006830828 145.0 2 CTTCGGC 10 0.006830828 145.0 8 CCCCACT 10 0.006830828 145.0 1 CACAGCT 10 0.006830828 145.0 3 ACAGCTT 10 0.006830828 145.0 4 AACCGTT 20 0.00593511 29.0 25-29 >>END_MODULE SRR6941541 read2 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR6941541_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 51 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 34.314 35.0 35.0 35.0 33.0 35.0 2 34.47075 35.0 35.0 35.0 33.0 35.0 3 34.4785 35.0 35.0 35.0 33.0 35.0 4 34.36725 35.0 35.0 35.0 33.0 35.0 5 34.4785 35.0 35.0 35.0 33.0 35.0 6 39.25025 40.0 40.0 40.0 39.0 40.0 7 39.2105 40.0 40.0 40.0 39.0 40.0 8 39.28925 40.0 40.0 40.0 39.0 40.0 9 39.3025 40.0 40.0 40.0 39.0 40.0 10-14 39.31034999999999 40.0 40.0 40.0 39.0 40.0 15-19 39.32195 40.0 40.0 40.0 39.0 40.0 20-24 39.337 40.0 40.0 40.0 39.0 40.0 25-29 39.29115 40.0 40.0 40.0 39.0 40.0 30-34 39.274249999999995 40.0 40.0 40.0 38.8 40.0 35-39 39.3038 40.0 40.0 40.0 39.0 40.0 40-44 39.192600000000006 40.0 40.0 40.0 38.6 40.0 45-49 39.0484 40.0 40.0 40.0 38.4 40.0 50-54 38.98635 40.0 39.4 40.0 38.0 40.0 55-59 39.0888 40.0 39.8 40.0 38.2 40.0 60-64 39.04425 40.0 39.6 40.0 38.0 40.0 65-69 38.92005 40.0 39.0 40.0 37.6 40.0 70-74 38.918099999999995 40.0 39.0 40.0 37.6 40.0 75-79 38.74865 40.0 39.0 40.0 36.6 40.0 80-84 38.79995 40.0 39.0 40.0 37.2 40.0 85-89 38.713750000000005 40.0 39.0 40.0 36.6 40.0 90-94 38.67815 40.0 39.0 40.0 36.6 40.0 95-99 38.5608 40.0 39.0 40.0 36.2 40.0 100-104 37.6767 39.0 38.0 39.6 34.4 39.8 105-109 38.3331 40.0 39.0 40.0 35.6 40.0 110-114 35.06475 36.4 34.8 38.4 30.2 38.6 115-119 19.015050000000002 16.6 16.0 23.4 13.4 30.0 120-124 2.0 2.0 2.0 2.0 2.0 2.0 125-129 2.0 2.0 2.0 2.0 2.0 2.0 130-134 2.0 2.0 2.0 2.0 2.0 2.0 135-139 2.0 2.0 2.0 2.0 2.0 2.0 140-144 2.0 2.0 2.0 2.0 2.0 2.0 145-149 2.0 2.0 2.0 2.0 2.0 2.0 150-151 2.0 2.0 2.0 2.0 2.0 2.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 0.0 4 0.0 5 0.0 6 1.0 7 0.0 8 1.0 9 0.0 10 1.0 11 1.0 12 0.0 13 1.0 14 1.0 15 0.0 16 0.0 17 2.0 18 6.0 19 7.0 20 11.0 21 17.0 22 12.0 23 36.0 24 36.0 25 57.0 26 54.0 27 108.0 28 146.0 29 292.0 30 2032.0 31 1177.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 46.36955433149725 15.498247371056584 7.436154231347021 30.696044066099148 2 32.191095547773884 22.18609304652326 25.962981490745374 19.65982991495748 3 24.73736868434217 23.66183091545773 30.06503251625813 21.53576788394197 4 29.664832416208103 29.514757378689342 21.435717858929465 19.384692346173086 5 31.065532766383193 30.515257628814407 18.684342171085543 19.734867433716857 6 26.474999999999998 35.675000000000004 17.275 20.575 7 23.5 23.5 30.349999999999998 22.650000000000002 8 25.074999999999996 23.05 25.4 26.474999999999998 9 29.849999999999998 21.224999999999998 25.224999999999998 23.7 10-14 28.685 25.94 22.115000000000002 23.26 15-19 29.2 25.285000000000004 22.66 22.855 20-24 29.415000000000003 25.869999999999997 21.985 22.73 25-29 29.975 25.35 22.27 22.405 30-34 29.325000000000003 26.02 21.88 22.775000000000002 35-39 29.759999999999998 27.125 21.415 21.7 40-44 29.345 26.77 21.5 22.384999999999998 45-49 28.59645610171188 27.009710681749926 21.178296125738314 23.215537090799877 50-54 29.422942294229422 25.657565756575657 22.29222922292229 22.627262726272626 55-59 28.463539061718517 26.377913374012202 22.64179253776133 22.516755026507955 60-64 29.296464823241163 25.351267563378173 22.78113905695285 22.571128556427823 65-69 30.320000000000004 25.545 21.64 22.495 70-74 29.54 26.515 22.32 21.625 75-79 29.82 25.009999999999998 22.025 23.145 80-84 30.19103820764153 25.745149029805965 22.854570914182837 21.209241848369672 85-89 29.93 25.509999999999998 22.17 22.39 90-94 29.409999999999997 26.355 21.16 23.075000000000003 95-99 29.99 25.39 22.415 22.205 100-104 29.73 26.985 21.555 21.73 105-109 29.909999999999997 25.130000000000003 23.35 21.61 110-114 30.114148392910785 25.458095524181434 22.969860819064785 21.457895263842996 115-119 30.39879608728367 26.768246802106848 22.18459994983697 20.64835716077251 120-124 27.373487871302803 41.11723351700551 14.218759896130218 17.29051871556147 125-129 NaN NaN NaN NaN 130-134 29.9598695761224 27.689992475545523 20.855279658891394 21.494858289440682 135-139 29.440072184069376 26.808361321369496 21.971026116597322 21.78054037796381 140-144 30.60762360045289 26.129072839350865 22.90854195496289 20.354761605233364 145-149 29.599999999999998 26.875 23.1 20.424999999999997 150-151 30.910683012259195 28.171128346259692 20.678008506379786 20.240180135101326 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.5 23 1.0 24 0.5 25 1.5 26 2.5 27 2.5 28 4.0 29 8.5 30 10.0 31 8.0 32 6.5 33 13.5 34 23.0 35 25.0 36 29.5 37 38.5 38 49.0 39 70.5 40 71.5 41 72.0 42 78.5 43 78.0 44 84.5 45 77.5 46 77.5 47 84.0 48 90.0 49 111.0 50 138.0 51 169.0 52 172.5 53 206.0 54 313.5 55 386.0 56 343.0 57 234.5 58 189.0 59 168.5 60 133.0 61 110.5 62 90.0 63 52.0 64 23.5 65 16.5 66 11.0 67 15.0 68 21.5 69 25.0 70 17.0 71 10.5 72 6.5 73 2.5 74 5.0 75 7.5 76 6.0 77 3.0 78 3.0 79 2.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 0.15 2 0.05 3 0.05 4 0.05 5 0.05 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.11 50-54 0.01 55-59 0.03 60-64 0.005 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.02 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.13 115-119 20.26 120-124 21.055 125-129 100.0 130-134 60.129999999999995 135-139 0.255 140-144 20.51 145-149 80.0 150-151 0.075 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 64.625 #Duplication Level Percentage of deduplicated Percentage of total 1 75.43520309477756 48.75 2 14.042553191489363 18.15 3 5.029013539651837 9.75 4 2.1663442940038684 5.6000000000000005 5 1.0831721470019342 3.5000000000000004 6 0.6963249516441006 2.7 7 0.425531914893617 1.925 8 0.23210831721470018 1.2 9 0.11605415860735009 0.675 >10 0.7736943907156674 7.75 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTTCGATCCTGGCTCAGGATGAACGCTGGCGGCATGCTTAACACATGCAA 44 1.0999999999999999 No Hit CCTTACCAGGGCTTGACATGCCGCGAATCCTCTTGAAAGAGAGGGGTGCC 29 0.7250000000000001 No Hit GGCTTGCGGTGGATACCTAGGCACCCAGAGACGAGGAAGGGCGTAGCAAG 26 0.65 No Hit CCTGCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGCAA 22 0.5499999999999999 No Hit GAACCTTACCAGGGCTTGACATGCCGCGAATCCTCTTGAAAGAGAGGGGT 16 0.4 No Hit GCCTGACGGAGCAATGCCGCGTGGAGGTGGAAGGCCTACGGGTCGTCAAC 15 0.375 No Hit AGAACCTTACCAGGGCTTGACATGCCGCGAATCCTCTTGAAAGAGAGGGG 15 0.375 No Hit CCTAGGCACCCAGAGACGAGGAAGGGCGTAGCAAGCGACGAAATGCTTCG 13 0.325 No Hit CTTGCGGTGGATACCTAGGCACCCAGAGACGAGGAAGGGCGTAGCAAGCG 12 0.3 No Hit GCATCGGCTAACTCTGTGCCAGCAGCCGCGGTAAGACAGAGGATGCAAGC 12 0.3 No Hit ATTATCTATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAAT 12 0.3 No Hit CGGGTGAGTAACGCGTAAGAACCTGCCCTTGGGAGGGGAACAACAACTGG 12 0.3 No Hit CAGTGACCAGGCCCGGGCGACTGTTTACCAAAAACACAGGTCTCCGCAAA 11 0.27499999999999997 No Hit GAAACAATGACGGTATCTGAGGAATAAGCATCGGCTAACTCTGTGCCAGC 11 0.27499999999999997 No Hit GGGAAACAGCCCGGATCACCAGCTAAGGCCCCTAAATGACCGCTCAGTGA 10 0.25 No Hit GGATGAACGCTGGCGGCATGCTTAACACATGCAAGTCGAACGGGAAGTGG 10 0.25 No Hit GGGAATTTCCGGTGGAGCGGTGAAATGCATTGAGATCGGAAAGAACACCA 10 0.25 No Hit GATGAACGCTGGCGGCATGCTTAACACATGCAAGTCGAACGGGAAGTGGT 10 0.25 No Hit CTTACCAGGTCCAGACATAGCAAGGATTGACAGACTGAGAGCTCTTTCTT 10 0.25 No Hit TATCTATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTT 10 0.25 No Hit GGCGGGACTACCCGCTGAGTTTAAGCATATAAATAAGCGGAGGAGAAGAA 9 0.22499999999999998 No Hit CTGACACTGAGAGACGAAAGCTAGGGGAGCAAATGGGATTAGAGACCCCA 9 0.22499999999999998 No Hit GCTTAACACATGCAAGTCGAACGGGAAGTGGTGTTTCCAGTGGCGAACGG 9 0.22499999999999998 No Hit CAGGGCTTGACATGCCGCGAATCCTCTTGAAAGAGAGGGGTGCCCTCGGG 8 0.2 No Hit CCTGGCTCAGGATGAACGCTGGCGGCATGCTTAACACATGCAAGTCGAAC 8 0.2 No Hit GTTAGCCGAAAGATGGTTATAGGTTTAAGGACACAAGGTGACCCTGCTTT 8 0.2 No Hit GGAAGGCCTACGGGTCGTCAACTTCTTTTCTCGGAGAAGAAACAATGACG 8 0.2 No Hit CTTACCAGGGCTTGACATGCCGCGAATCCTCTTGAAAGAGAGGGGTGCCC 8 0.2 No Hit CGATCCTGGCTCAGGATGAACGCTGGCGGCATGCTTAACACATGCAAGTC 8 0.2 No Hit GTTGCTAATACCCCGTAGGCTGAGGAGCAAAAGGAGAAATCCGCCCAAGG 7 0.17500000000000002 No Hit CTCAGGATGAACGCTGGCGGCATGCTTAACACATGCAAGTCGAACGGGAA 7 0.17500000000000002 No Hit GTTGAAGAATGAGCCGGCGACTCATAGGCAGTGGCTTGGTTAAGGGAACG 7 0.17500000000000002 No Hit GCCGCGAATCCTCTTGAAAGAGAGGGGTGCCCTCGGGAACGCGGACACAG 7 0.17500000000000002 No Hit GCTTGCGGTGGATACCTAGGCACCCAGAGACGAGGAAGGGCGTAGCAAGC 7 0.17500000000000002 No Hit GTGGGAACTTAGTTTCCGTTTGGGTATGCGCCCTTGGATTGCTGTTGCAT 7 0.17500000000000002 No Hit GATCCTGGCTCAGGATGAACGCTGGCGGCATGCTTAACACATGCAAGTCG 7 0.17500000000000002 No Hit GGTGACCCTGCTTTTTCAGGGTAAGAAGGGGTAGAGAAAATGCCTCGAGC 7 0.17500000000000002 No Hit CTCGTGTTTAGTTGCCACTATGAGTTTGGAACCCTGAACAGACCGCCGGT 7 0.17500000000000002 No Hit ACCTTACCAGGGCTTGACATGCCGCGAATCCTCTTGAAAGAGAGGGGTGC 7 0.17500000000000002 No Hit GGAAAAGAAAGCAAAAGCGATTCCCGTAGTAGCGGCGAGCGAAATGGGAG 7 0.17500000000000002 No Hit GGCTGTCGTCAGCTCGTGCCGTAAGGTGTTGGGTTAAGTCTCGCAACGAG 6 0.15 No Hit GTCTGGTGCCAGCAGCCGCGGTAATTCCAGCTCCAATAGCGTATATTTAA 6 0.15 No Hit CACTGACACTGAGAGACGAAAGCTAGGGGAGCAAATGGGATTAGAGACCC 6 0.15 No Hit GTTAAGTCTCGCAACGAGCGCAACCCTCGTGTTTAGTTGCCACTATGAGT 6 0.15 No Hit GGAAAGAACACCAACGGCGAAAGCACTCTGCTGGGCCGACACTGACACTG 6 0.15 No Hit CATGCCGCGAATCCTCTTGAAAGAGAGGGGTGCCCTCGGGAACGCGGACA 6 0.15 No Hit GGTCAGGCGGGACTACCCGCTGAGTTTAAGCATATAAATAAGCGGAGGAG 6 0.15 No Hit GGCCTCCCAAAAGGTAACGGAGGCGTGCAAAGGTTTCCTCGGGCCAGACG 6 0.15 No Hit GTTATTTTGGCCGATTAATCTTCCAATATGCTAGTTTCAACAACTCTCGT 6 0.15 No Hit GGTGAAATGCATTGAGATCGGAAAGAACACCAACGGCGAAAGCACTCTGC 6 0.15 No Hit CAATAGCTTACCAAGGCGATGATCAGTAGCTGGTCCGAGAGGATGATCAG 6 0.15 No Hit TGAACGCTGGCGGCATGCTTAACACATGCAAGTCGAACGGGAAGTGGTGT 6 0.15 No Hit GACAGAGGATGCAAGCGTTATCCGGAATGATTGGGCGTAAAGCGTCTGTA 6 0.15 No Hit CTTTGGGCCGGGTCGGCCGGTCCGCCTCACGGCGAGCACCGACCTACTCG 6 0.15 No Hit GGTAGGGGCAGAGGGAATTTCCGGTGGAGCGGTGAAATGCATTGAGATCG 6 0.15 No Hit AGTGACCAGGCCCGGGCGACTGTTTACCAAAAACACAGGTCTCCGCAAAG 6 0.15 No Hit CGAAAACATTGGTGAGAATCCAATGCCCCGAAAACCCAAGGTTTCCTCCG 6 0.15 No Hit GTGAAATAGAACGTGAAACCGTGCTGAGCTCCCAAGCAGTGGGAGGGGAA 6 0.15 No Hit AAGCGTCTGTAGGTGGCTTTTCAAGTCCGCCGTCAAATCCCAGGGCTCAA 5 0.125 No Hit GGTTAGCCGAAAGATGGTTATAGGTTTAAGGACACAAGGTGACCCTGCTT 5 0.125 No Hit GGTGAAATGCCACTCGAACCCAGAGCTAGCTGGTTCTCCCCGAAATGCGT 5 0.125 No Hit CGGGAAGTGGTGTTTCCAGTGGCGAACGGGTGAGTAACGCGTAAGAACCT 5 0.125 No Hit GGAAAGGTGAAAAGAACCCCCAGTGGGTAGTGAAATAGAACGTGAAACCG 5 0.125 No Hit CCTCTTGAAAGAGAGGGGTGCCCTCGGGAACGCGGACACAGGTGGTGCAT 5 0.125 No Hit GGCTTGACATGCCGCGAATCCTCTTGAAAGAGAGGGGTGCCCTCGGGAAC 5 0.125 No Hit CTGGAAAGGGGCGCCTGGGAGGGTGAGAGCCCCGTCCGGCCCGGACCCTG 5 0.125 No Hit ACCAGGTCCAGACATAGCAAGGATTGACAGACTGAGAGCTCTTTCTTGAT 5 0.125 No Hit CTGCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGCAAG 5 0.125 No Hit GTTTAAGGACACAAGGTGACCCTGCTTTTTCAGGGTAAGAAGGGGTAGAG 5 0.125 No Hit GTACGGTAGGGGCAGAGGGAATTTCCGGTGGAGCGGTGAAATGCATTGAG 5 0.125 No Hit GCTTGTTATATGGGTCGTGAGTGGGAACTTAGTTTCCGTTTGGGTATGCG 5 0.125 No Hit CTCGGGAACGCGGACACAGGTGGTGCATGGCTGTCGTCAGCTCGTGCCGT 5 0.125 No Hit AGGGAATTTCCGGTGGAGCGGTGAAATGCATTGAGATCGGAAAGAACACC 5 0.125 No Hit GTCAAAATGCATCGGTAGGGGAGCGTTCCGCCTTAGAGGGAAGCAACCGC 5 0.125 No Hit GCCTCCCAAAAGGTAACGGAGGCGTGCAAAGGTTTCCTCGGGCCAGACGG 5 0.125 No Hit CATCGGCTAACTCTGTGCCAGCAGCCGCGGTAAGACAGAGGATGCAAGCG 5 0.125 No Hit GACAAAGGGTCGCGATCTCGCGAGGGTGAGCTAACTCCAAAAACCCGTCC 5 0.125 No Hit GAACAGTCGACTCAGAACTGGTACGGACAAGGGGAATCCGACTGTTTAAT 5 0.125 No Hit CACACGTGCTACAATGGGCGGGACAAAGGGTCGCGATCTCGCGAGGGTGA 5 0.125 No Hit TGACATGCCGCGAATCCTCTTGAAAGAGAGGGGTGCCCTCGGGAACGCGG 5 0.125 No Hit GTTGGGTTAAGTCTCGCAACGAGCGCAACCCTCGTGTTTAGTTGCCACTA 5 0.125 No Hit CCCAAATAGGTCAACCTTTTAAACTGCCTGCTGAATCCATGAGCAGGCAA 5 0.125 No Hit GAGTTCGATCCTGGCTCAGGATGAACGCTGGCGGCATGCTTAACACATGC 5 0.125 No Hit AATGCATTGAGATCGGAAAGAACACCAACGGCGAAAGCACTCTGCTGGGC 5 0.125 No Hit CTCATAGGCAGTGGCTTGGTTAAGGGAACGGAACCCACCGGAGCCGTAGC 5 0.125 No Hit CGGGTCGTCAACTTCTTTTCTCGGAGAAGAAACAATGACGGTATCTGAGG 5 0.125 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.025 0.0 0.0 0.0 0.0 2 0.025 0.0 0.0 0.0 0.0 3 0.025 0.0 0.0 0.0 0.0 4 0.025 0.0 0.0 0.0 0.0 5 0.025 0.0 0.0 0.0 0.0 6 0.025 0.0 0.0 0.0 0.0 7 0.025 0.0 0.0 0.0 0.0 8 0.025 0.0 0.0 0.0 0.0 9 0.025 0.0 0.0 0.0 0.0 10-11 0.025 0.0 0.0 0.0 0.0 12-13 0.025 0.0 0.0 0.0 0.0 14-15 0.025 0.0 0.0 0.0 0.0 16-17 0.025 0.0 0.0 0.0 0.0 18-19 0.025 0.0 0.0 0.0 0.0 20-21 0.025 0.0 0.0 0.0 0.0 22-23 0.025 0.0 0.0 0.0 0.0 24-25 0.025 0.0 0.0 0.0 0.0 26-27 0.025 0.0 0.0 0.0 0.0 28-29 0.025 0.0 0.0 0.0 0.0 30-31 0.025 0.0 0.0 0.0 0.0 32-33 0.025 0.0 0.0 0.0 0.0 34-35 0.025 0.0 0.0 0.0 0.0 36-37 0.025 0.0 0.0 0.0 0.0 38-39 0.05 0.0 0.0 0.0 0.0 40-41 0.05 0.0 0.0 0.0 0.0 42-43 0.05 0.0 0.0 0.0 0.0 44-45 0.05 0.0 0.0 0.0 0.0 46-47 0.0625 0.0 0.0 0.0 0.0 48-49 0.1 0.0 0.0 0.0 0.0 50-51 0.125 0.0 0.0 0.0 0.0 52-53 0.1375 0.0 0.0 0.0 0.0 54-55 0.2 0.0 0.0 0.0 0.0 56-57 0.2375 0.0 0.0 0.0 0.0 58-59 0.3125 0.0 0.0 0.0 0.0 60-61 0.375 0.0 0.0 0.0 0.0 62-63 0.5 0.0 0.0 0.0 0.0 64-65 0.55 0.0 0.0 0.0 0.0 66-67 0.6125 0.0 0.0 0.0 0.0 68-69 0.7375 0.0 0.0 0.0 0.0 70-71 0.8 0.0 0.0 0.0 0.0 72-73 0.8875 0.0 0.0 0.0 0.0 74-75 1.15 0.0 0.0 0.0 0.0 76-77 1.4 0.0 0.0 0.0 0.0 78-79 1.625 0.0 0.0 0.0 0.0 80-81 1.9125 0.0 0.0 0.0 0.0 82-83 2.1375 0.0 0.0 0.0 0.0 84-85 2.3499999999999996 0.0 0.0 0.0 0.0 86-87 2.725 0.0 0.0 0.0 0.0 88-89 3.125 0.0 0.0 0.0 0.0 90-91 3.7 0.0 0.0 0.0 0.0 92-93 4.15 0.0 0.0 0.0 0.0 94-95 4.85 0.0 0.0 0.0 0.0 96-97 5.425 0.0 0.0 0.0 0.0 98-99 6.112500000000001 0.0 0.0 0.0 0.0 100-101 6.7625 0.0 0.0 0.0 0.0 102-103 7.2625 0.0 0.0 0.0 0.0 104-105 7.824999999999999 0.0 0.0 0.0 0.0 106-107 8.4375 0.0 0.0 0.0 0.0 108-109 8.575 0.0 0.0 0.0 0.0 110-111 8.575 0.0 0.0 0.0 0.0 112-113 8.575 0.0 0.0 0.0 0.0 114-115 8.575 0.0 0.0 0.0 0.0 116-117 8.575 0.0 0.0 0.0 0.0 118-119 8.575 0.0 0.0 0.0 0.0 120-121 8.575 0.0 0.0 0.0 0.0 122-123 8.575 0.0 0.0 0.0 0.0 124-125 8.575 0.0 0.0 0.0 0.0 126-127 8.575 0.0 0.0 0.0 0.0 128-129 8.575 0.0 0.0 0.0 0.0 130-131 8.575 0.0 0.0 0.0 0.0 132-133 8.575 0.0 0.0 0.0 0.0 134-135 8.575 0.0 0.0 0.0 0.0 136-137 8.575 0.0 0.0 0.0 0.0 138-139 8.575 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATCCTGG 15 3.0800808E-4 113.0 6 GATCCTG 15 3.0800808E-4 113.0 5 TCCTGGC 15 3.0800808E-4 113.0 7 CCTGGCT 15 3.0800808E-4 113.0 8 CTGGCTC 20 9.6693676E-4 84.75 9 >>END_MODULE Read 1253180 spots for SRR6941541.sra Written 1253180 spots for SRR6941541.sra Read 1253180 spots for SRR6941541.sra Written 1253180 spots for SRR6941541.sra Read 1253180 spots for SRR6941541.sra Written 1253180 spots for SRR6941541.sra Read 1253180 spots for SRR6941541.sra Written 1253180 spots for SRR6941541.sra Read 1253180 spots for SRR6941541.sra Written 1253180 spots for SRR6941541.sra Read 1253180 spots for SRR6941541.sra Written 1253180 spots for SRR6941541.sra Read 1253180 spots for SRR6941541.sra Written 1253180 spots for SRR6941541.sra Read 1253180 spots for SRR6941541.sra Written 1253180 spots for SRR6941541.sra Read 1253180 spots for SRR6941541.sra Written 1253180 spots for SRR6941541.sra Read 1253180 spots for SRR6941541.sra Written 1253180 spots for SRR6941541.sra Read 1253180 spots for SRR6941541.sra Written 1253180 spots for SRR6941541.sra Read 1253180 spots for SRR6941541.sra Written 1253180 spots for SRR6941541.sra Read 1253180 spots for SRR6941541.sra Written 1253180 spots for SRR6941541.sra Read 1253180 spots for SRR6941541.sra Written 1253180 spots for SRR6941541.sra Read 1253180 spots for SRR6941541.sra Written 1253180 spots for SRR6941541.sra Read 1253180 spots for SRR6941541.sra Written 1253180 spots for SRR6941541.sra Read 1253180 spots for SRR6941541.sra Written 1253180 spots for SRR6941541.sra Read 1253180 spots for SRR6941541.sra Written 1253180 spots for SRR6941541.sra Read 1253180 spots for SRR6941541.sra Written 1253180 spots for SRR6941541.sra Read 1253180 spots for SRR6941541.sra Written 1253180 spots for SRR6941541.sra SRR ids: ['SRR6941541.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_zde64pj5 SRR6941541.sra spots: 25063600 blocks: [[1, 1253180], [1253181, 2506360], [2506361, 3759540], [3759541, 5012720], [5012721, 6265900], [6265901, 7519080], [7519081, 8772260], [8772261, 10025440], [10025441, 11278620], [11278621, 12531800], [12531801, 13784980], [13784981, 15038160], [15038161, 16291340], [16291341, 17544520], [17544521, 18797700], [18797701, 20050880], [20050881, 21304060], [21304061, 22557240], [22557241, 23810420], [23810421, 25063600]] SRR6941541 file size 8471531 SRR6941541 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR6941541 SRR6941541_1.fastq SRR6941541_2.fastq Input file: SRR6941541_1.fastq Paired file: SRR6941541_2.fastq trimmed: SRR6941541-trimmed-pair1.fastq, SRR6941541-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Fri Dec 6 10:50:28 2024 >> started Fri Dec 6 10:50:58 2024 >> done (29.878s) 25063600 read pairs processed; of these: 24583 ( 0.10%) short read pairs filtered out after trimming by size control 19903 ( 0.08%) empty read pairs filtered out after trimming by size control 25019114 (99.82%) read pairs available; of these: 21315864 (85.20%) trimmed read pairs available after processing 3703250 (14.80%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 12 0.00% 19 19 0.00% 20 12 0.00% 21 16 0.00% 22 26 0.00% 23 24 0.00% 24 30 0.00% 25 35 0.00% 26 61 0.00% 27 79 0.00% 28 95 0.00% 29 123 0.00% 30 145 0.00% 31 162 0.00% 32 202 0.00% 33 194 0.00% 34 243 0.00% 35 295 0.00% 36 333 0.00% 37 336 0.00% 38 392 0.00% 39 473 0.00% 40 517 0.00% 41 627 0.00% 42 609 0.00% 43 722 0.00% 44 800 0.00% 45 865 0.00% 46 969 0.00% 47 1024 0.00% 48 1144 0.00% 49 1379 0.01% 50 1508 0.01% 51 1773 0.01% 52 2133 0.01% 53 2404 0.01% 54 2661 0.01% 55 2786 0.01% 56 2976 0.01% 57 3466 0.01% 58 4063 0.02% 59 4377 0.02% 60 4794 0.02% 61 6519 0.03% 62 7569 0.03% 63 7491 0.03% 64 8366 0.03% 65 9564 0.04% 66 10046 0.04% 67 10627 0.04% 68 13183 0.05% 69 14543 0.06% 70 16470 0.07% 71 17055 0.07% 72 20857 0.08% 73 22655 0.09% 74 21547 0.09% 75 24219 0.10% 76 26005 0.10% 77 29020 0.12% 78 29836 0.12% 79 33346 0.13% 80 36090 0.14% 81 37924 0.15% 82 41152 0.16% 83 42573 0.17% 84 44609 0.18% 85 54384 0.22% 86 59240 0.24% 87 59397 0.24% 88 67813 0.27% 89 60478 0.24% 90 60983 0.24% 91 65223 0.26% 92 68141 0.27% 93 74746 0.30% 94 81753 0.33% 95 72844 0.29% 96 70274 0.28% 97 75239 0.30% 98 73141 0.29% 99 73983 0.30% 100 75185 0.30% 101 78521 0.31% 102 84611 0.34% 103 83297 0.33% 104 91047 0.36% 105 86471 0.35% 106 89567 0.36% 107 90797 0.36% 108 88129 0.35% 109 112101 0.45% 110 91679 0.37% 111 99627 0.40% 112 120591 0.48% 113 85647 0.34% 114 99525 0.40% 115 96763 0.39% 116 121379 0.49% 117 120632 0.48% 118 116354 0.47% 119 135903 0.54% 120 145369 0.58% 121 140611 0.56% 122 139318 0.56% 123 152384 0.61% 124 153028 0.61% 125 159998 0.64% 126 160854 0.64% 127 171132 0.68% 128 193570 0.77% 129 257661 1.03% 130 453630 1.81% 131 882553 3.53% 132 700483 2.80% 133 3531565 14.12% 134 4413125 17.64% 135 87523 0.35% 136 72141 0.29% 137 75579 0.30% 138 85804 0.34% 139 119635 0.48% 140 528991 2.11% 141 1350164 5.40% 142 239705 0.96% 143 1731516 6.92% 144 127334 0.51% 145 525473 2.10% 146 42258 0.17% 147 51926 0.21% 148 63960 0.26% 149 102748 0.41% 150 894191 3.57% 151 3703250 14.80% 25019114 reads passed initial QC criterion=sequence-density sequence-density=3.83 sequence-density-rank=1 fanout-score=1.92 fanout-score-rank=30 prefix-density=3.77 prefix-fanout=1.9 sequence=GTATTTAGCCTTG criterion=fanout-score sequence-density=0.07 sequence-density-rank=22 fanout-score=70.94 fanout-score-rank=1 prefix-density=5.28 prefix-fanout=1.0 sequence=GCTGATCATCCGAAAAGACCAGCTAAGCATCATTGGCTTGGTCAGCCTTTACCTGACCAACTACCTAATACTACGCAGGCTCATCAAACAGCGCTTTTGAGCTTTCTTCAGGATTTGGCCCGAACTGTTCGGCAGATTCCCACGCGTTACGCACCCGTTCGC criterion=sequence-density sequence-density=0.89 sequence-density-rank=1 fanout-score=7.63 fanout-score-rank=12 prefix-density=4.72 prefix-fanout=1.4 sequence=TGGTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGTTAACGAACGAGACCTCAGCCTGCTAACTAGCTATGCGGAGCCATCCCTCCGCAGCTAGCTTCTTAGAGGGACTATCGCCGTTTAGGCGACGGAAGTTTGAGGCAATAACAGGTCTGTGATGCCCTTAGATGTTCTGGGCCGCACGCGCGCTACACTGATGTATTCAACGAGTATATAGCCTTGGCCGACAGGCCCGGGTAATCTTGGGAAATTTCATCGTGATGGGGATAGATCATTGCAATTGTTGGTCTTCAACGAGGAATGCCTAGTAAGCGCGAGTCATCAGCTCGCGTTGACTACGTCCCTGCCCTTTGTACACACC criterion=fanout-score sequence-density=0.02 sequence-density-rank=33 fanout-score=116.64 fanout-score-rank=1 prefix-density=2.47 prefix-fanout=1.0 sequence=CGCGGTAAGACGGGGGGGGCAAGTGTTCTTCGGAATGACTGGGCGTAAAGGGCACGTAGGCGGTGAATCGGGTTGAAAGTGAAAGTCGCCAAAAAGTGGCGGAATGCTCTCGAAACCAATTCACTTGAGTGAGACAGAGGAGAGTGGAATTTCGTGTGTAGGGGTGAAA Potential 3prime adapter identified. Now checking if in reference sequence /dee2/code/volunteer_pipeline.sh: line 905: -f: command not found /dee2/code/volunteer_pipeline.sh: line 906: -f: command not found Adapter seq not found in reference. Now shuffling file before clipping skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -t 20 -x GTATTTAGCCTTG -y TGGTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGTTAACGAACGAGACCTCAGCCTGCTAACTAGCTATGCGGAGCCATCCCTCCGCAGCTAGCTTCTTAGAGGGACTATCGCCGTTTAGGCGACGGAAGTTTGAGGCAATAACAGGTCTGTGATGCCCTTAGATGTTCTGGGCCGCACGCGCGCTACACTGATGTATTCAACGAGTATATAGCCTTGGCCGACAGGCCCGGGTAATCTTGGGAAATTTCATCGTGATGGGGATAGATCATTGCAATTGTTGGTCTTCAACGAGGAATGCCTAGTAAGCGCGAGTCATCAGCTCGCGTTGACTACGTCCCTGCCCTTTGTACACACC -o SRR6941541 SRR6941541_1.fastq SRR6941541_2.fastq Input file: SRR6941541_1.fastq Paired file: SRR6941541_2.fastq trimmed: SRR6941541-trimmed-pair1.fastq, SRR6941541-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): GTATTTAGCCTTG -- paired 3' end adapter sequence (-y): TGGTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGTTAACGAACGAGAC -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Fri Dec 6 10:54:13 2024 >> started Fri Dec 6 10:54:22 2024 >> done (9.394s) 8339705 read pairs processed; of these: 342 ( 0.00%) short read pairs filtered out after trimming by size control 591 ( 0.01%) empty read pairs filtered out after trimming by size control 8338772 (99.99%) read pairs available; of these: 9411 ( 0.11%) trimmed read pairs available after processing 8329361 (99.89%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 4 0.00% 19 6 0.00% 20 6 0.00% 21 6 0.00% 22 10 0.00% 23 9 0.00% 24 11 0.00% 25 11 0.00% 26 23 0.00% 27 20 0.00% 28 28 0.00% 29 46 0.00% 30 44 0.00% 31 40 0.00% 32 68 0.00% 33 47 0.00% 34 73 0.00% 35 117 0.00% 36 125 0.00% 37 111 0.00% 38 138 0.00% 39 155 0.00% 40 174 0.00% 41 198 0.00% 42 228 0.00% 43 224 0.00% 44 257 0.00% 45 295 0.00% 46 316 0.00% 47 346 0.00% 48 382 0.00% 49 470 0.01% 50 515 0.01% 51 605 0.01% 52 745 0.01% 53 799 0.01% 54 908 0.01% 55 880 0.01% 56 1000 0.01% 57 1149 0.01% 58 1352 0.02% 59 1488 0.02% 60 1577 0.02% 61 2125 0.03% 62 2558 0.03% 63 2520 0.03% 64 2758 0.03% 65 3121 0.04% 66 3308 0.04% 67 3446 0.04% 68 4361 0.05% 69 4910 0.06% 70 5598 0.07% 71 5603 0.07% 72 6928 0.08% 73 7431 0.09% 74 7162 0.09% 75 8212 0.10% 76 8645 0.10% 77 9591 0.12% 78 9841 0.12% 79 11036 0.13% 80 11795 0.14% 81 12609 0.15% 82 13731 0.16% 83 14098 0.17% 84 14847 0.18% 85 18218 0.22% 86 19635 0.24% 87 19527 0.23% 88 22420 0.27% 89 20280 0.24% 90 20194 0.24% 91 21730 0.26% 92 22712 0.27% 93 24840 0.30% 94 27221 0.33% 95 24002 0.29% 96 23326 0.28% 97 24721 0.30% 98 24511 0.29% 99 24676 0.30% 100 24704 0.30% 101 26002 0.31% 102 28384 0.34% 103 27813 0.33% 104 30288 0.36% 105 28943 0.35% 106 29927 0.36% 107 30357 0.36% 108 29527 0.35% 109 37356 0.45% 110 30486 0.37% 111 33357 0.40% 112 40539 0.49% 113 28087 0.34% 114 33384 0.40% 115 31975 0.38% 116 43029 0.52% 117 38515 0.46% 118 40171 0.48% 119 43301 0.52% 120 48336 0.58% 121 46686 0.56% 122 46586 0.56% 123 50828 0.61% 124 51427 0.62% 125 53384 0.64% 126 53721 0.64% 127 56784 0.68% 128 64803 0.78% 129 85822 1.03% 130 150780 1.81% 131 294722 3.53% 132 233667 2.80% 133 1176545 14.11% 134 1471368 17.64% 135 30062 0.36% 136 23108 0.28% 137 25204 0.30% 138 28583 0.34% 139 40077 0.48% 140 176220 2.11% 141 450784 5.41% 142 79934 0.96% 143 576706 6.92% 144 42062 0.50% 145 175015 2.10% 146 14295 0.17% 147 17227 0.21% 148 21177 0.25% 149 34318 0.41% 150 297910 3.57% 151 1235233 14.81% criterion=sequence-density sequence-density=3.82 sequence-density-rank=1 fanout-score=1.92 fanout-score-rank=31 prefix-density=3.76 prefix-fanout=1.9 sequence=GTATTTAGCCTTG criterion=fanout-score sequence-density=0.07 sequence-density-rank=22 fanout-score=71.55 fanout-score-rank=1 prefix-density=5.28 prefix-fanout=1.0 sequence=GCTGATCATCCGAAAAGACCAGCTAAGCATCATTGGCTTGGTCAGCCTTTACCTGACCAACTACCTAATACTACGCAGGCTCATCAAACAGCGCTTTTGAGCTTTCTTCAGGATTTGGCCCGAACTGTTCGGCAGATTCCCACGCGTTACGCACCCGTTCGC criterion=sequence-density sequence-density=0.89 sequence-density-rank=1 fanout-score=7.67 fanout-score-rank=12 prefix-density=4.75 prefix-fanout=1.4 sequence=TGGTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGTTAACGAACGAGACCTCAGCCTGCTAACTAGCTATGCGGAGCCATCCCTCCGCAGCTAGCTTCTTAGAGGGACTATCGCCGTTTAGGCGACGGAAGTTTGAGGCAATAACAGGTCTGTGATGCCCTTAGATGTTCTGGGCCGCACGCGCGCTACACTGATGTATTCAACGAGTATATAGCCTTGGCCGACAGGCCCGGGTAATCTTGGGAAATTTCATCGTGATGGGGATAGATCATTGCAATTGTTGGTCTTCAACGAGGAATGCCTAGTAAGCGCGAGTCATCAGCTCGCGTTGACTACGTCCCTGCCCTTTGTACACACC criterion=fanout-score sequence-density=0.01 sequence-density-rank=44 fanout-score=263.66 fanout-score-rank=1 prefix-density=1.45 prefix-fanout=1.0 sequence=TAGCGGCGAGACGAGCCGTTTAAATAGGTGTCAAGTGGAAGTGCAGTGATGTATGCAGCTGAGGCATCCTAACGAACGAACG SRR6941541 testing PE reads STAR mapping to Ensembl genome Started job on | Dec 06 10:55:07 Started mapping on | Dec 06 10:55:07 Finished on | Dec 06 10:56:58 Mapping speed, Million of reads per hour | 811.40 Number of input reads | 25018181 Average input read length | 266 UNIQUE READS: Uniquely mapped reads number | 9150228 Uniquely mapped reads % | 36.57% Average mapped length | 273.15 Number of splices: Total | 1030009 Number of splices: Annotated (sjdb) | 778266 Number of splices: GT/AG | 834411 Number of splices: GC/AG | 12537 Number of splices: AT/AC | 3711 Number of splices: Non-canonical | 179350 Mismatch rate per base, % | 0.28% Deletion rate per base | 0.00% Deletion average length | 1.50 Insertion rate per base | 0.00% Insertion average length | 1.73 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 10204662 % of reads mapped to multiple loci | 40.79% Number of reads mapped to too many loci | 826066 % of reads mapped to too many loci | 3.30% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 3.67% % of reads unmapped: other | 15.66% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 5673407 5673407 5673407 N_multimapping 10204662 10204662 10204662 N_noFeature 6852004 9027250 6908127 N_ambiguous 157971 3465 91465 UnstrandedReadsAssigned:2140253 PositiveStrandReadsAssigned:119513 NegativeStrandReadsAssigned:2150636 Dataset is classified negative stranded MeadianReadLen=151 20thPercentileLength=138 echo kmer=133 SRR6941541 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,972 [index] number of k-mers: 66,720,672 [index] number of equivalence classes: 111,837 [quant] running in paired-end mode [quant] will process pair 1: SRR6941541-trimmed-pair1.fastq SRR6941541-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 25,018,181 reads, 6,184,759 reads pseudoaligned [quant] estimated average fragment length: 186.917 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 970 rounds 52973 SRR6941541.ke.tsv 35125 SRR6941541.se.tsv 88098 total ==> SRR6941541.ke.tsv <== target_id length eff_length est_counts tpm PNS24245 936 750.41 4.0047 0.320181 PNS24247 1044 858.083 0 0 PNS24249 1928 1742.08 6.06405 0.208842 PNS24246 1044 858.083 0 0 PNS24248 1044 858.083 0 0 PNS24244 1471 1285.08 10.9312 0.510343 PNS24243 293 118.956 0 0 KQK14069 1603 1417.08 564.363 23.8939 KQK14071 474 289.517 38.2573 7.92803 ==> SRR6941541.se.tsv <== BRADI_1g14170v3 680 BRADI_1g53295v3 10 BRADI_1g59795v3 5 BRADI_1g07683v3 0 BRADI_1g00485v3 0 BRADI_1g20270v3 6 BRADI_1g74790v3 9 BRADI_1g09890v3 0 BRADI_1g77505v3 8 BRADI_1g48960v3 0 SRR6941541 completed mapping pipeline successfully