Starting /dee2/code/volunteer_pipeline.sh SRR6941542 current disk space = 1551468597248 free memory = 1600853412 SRR6941542 SRAfilesize 6dcc2a1e8887cacb16c797e31da63ea6 SRR6941542.sra SRR6941542.sra file validated SRR6941542 is paired end SRR6941542 is conventional basespace SRR6941542 read1 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR6941542_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 34.6775 35.0 35.0 35.0 35.0 35.0 2 34.627 35.0 35.0 35.0 35.0 35.0 3 34.703 35.0 35.0 35.0 35.0 35.0 4 34.7765 35.0 35.0 35.0 35.0 35.0 5 34.70025 35.0 35.0 35.0 35.0 35.0 6 39.63775 40.0 40.0 40.0 39.0 40.0 7 39.589 40.0 40.0 40.0 39.0 40.0 8 39.55725 40.0 40.0 40.0 39.0 40.0 9 39.59425 40.0 40.0 40.0 39.0 40.0 10-14 39.56635 40.0 40.0 40.0 39.0 40.0 15-19 39.5305 40.0 40.0 40.0 39.0 40.0 20-24 39.546049999999994 40.0 40.0 40.0 39.0 40.0 25-29 39.54025 40.0 40.0 40.0 39.0 40.0 30-34 39.439099999999996 40.0 40.0 40.0 39.0 40.0 35-39 39.5687 40.0 40.0 40.0 39.0 40.0 40-44 39.550399999999996 40.0 40.0 40.0 39.0 40.0 45-49 39.52825 40.0 40.0 40.0 39.0 40.0 50-54 39.4283 40.0 40.0 40.0 39.0 40.0 55-59 39.42909999999999 40.0 40.0 40.0 39.0 40.0 60-64 39.459250000000004 40.0 40.0 40.0 39.0 40.0 65-69 39.415299999999995 40.0 40.0 40.0 39.0 40.0 70-74 39.362300000000005 40.0 40.0 40.0 38.8 40.0 75-79 39.2365 40.0 40.0 40.0 38.8 40.0 80-84 39.311099999999996 40.0 40.0 40.0 39.0 40.0 85-89 39.29260000000001 40.0 40.0 40.0 39.0 40.0 90-94 39.2361 40.0 40.0 40.0 38.8 40.0 95-99 39.2086 40.0 40.0 40.0 38.8 40.0 100-104 38.30945 39.2 38.6 39.4 36.8 39.8 105-109 39.1892 40.0 40.0 40.0 38.8 40.0 110-114 39.26145 40.0 40.0 40.0 38.8 40.0 115-119 39.2894 40.0 40.0 40.0 39.0 40.0 120-124 39.1175 40.0 40.0 40.0 38.8 40.0 125-129 39.0331 40.0 39.4 40.0 38.4 40.0 130-134 39.0557 40.0 39.2 40.0 38.2 40.0 135-139 38.94775 40.0 39.0 40.0 37.8 40.0 140-144 38.7453 40.0 39.0 40.0 36.8 40.0 145-149 38.83135 40.0 39.0 40.0 37.6 40.0 150-151 37.225625 39.5 37.5 40.0 33.5 40.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 19 1.0 20 1.0 21 0.0 22 0.0 23 0.0 24 0.0 25 1.0 26 6.0 27 2.0 28 7.0 29 8.0 30 16.0 31 27.0 32 22.0 33 34.0 34 46.0 35 62.0 36 78.0 37 116.0 38 291.0 39 3282.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 57.754948634427464 10.298170884490103 4.810824354798296 27.136056126284142 2 21.975 12.325 30.925000000000004 34.775 3 22.05 20.674999999999997 26.5 30.775000000000002 4 25.124999999999996 26.825 21.075 26.974999999999998 5 25.169045830202858 33.88429752066116 22.364137240170297 18.582519408965688 6 19.2 38.15 21.95 20.7 7 14.549999999999999 30.599999999999998 39.5 15.35 8 17.224999999999998 28.275 30.425 24.075 9 16.55 25.424999999999997 35.449999999999996 22.575 10-14 20.485 33.105000000000004 24.9 21.51 15-19 21.63 30.819999999999997 25.174999999999997 22.375 20-24 18.78687868786879 31.63816381638164 26.387638763876385 23.187318731873187 25-29 22.23 30.975 26.13 20.665 30-34 21.815 33.050000000000004 23.974999999999998 21.16 35-39 21.115000000000002 30.925000000000004 26.035000000000004 21.925 40-44 18.845 30.904999999999998 26.695 23.555 45-49 19.950000000000003 30.385 27.73 21.935 50-54 21.065 31.009999999999998 25.72 22.205 55-59 20.715 30.275000000000002 25.974999999999998 23.035 60-64 18.335 30.930000000000003 26.979999999999997 23.755000000000003 65-69 20.13 31.655 25.974999999999998 22.24 70-74 20.375 30.435000000000002 25.064999999999998 24.125 75-79 20.43 29.425 26.865 23.28 80-84 21.63 29.99 25.8 22.58 85-89 21.65 29.145 26.63 22.575 90-94 18.575 30.955 26.784999999999997 23.685000000000002 95-99 20.025000000000002 31.240000000000002 24.15 24.585 100-104 20.115 32.26 24.855 22.770000000000003 105-109 20.035 31.230000000000004 25.53 23.205000000000002 110-114 21.12 29.535 25.480000000000004 23.865 115-119 20.419999999999998 31.52 24.04 24.02 120-124 20.387426168785662 30.919010912003202 23.99139052958254 24.70217238962859 125-129 20.23202320232023 31.113111311131114 24.137413741374136 24.51745174517452 130-134 21.37 31.619999999999997 23.77 23.24 135-139 22.08 31.415 23.925 22.58 140-144 22.935 29.69 25.515 21.86 145-149 20.555 31.225 24.279999999999998 23.94 150-151 21.109302616752224 32.352572931012894 22.161011643921373 24.37711280831351 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.5 18 0.5 19 1.5 20 1.5 21 1.0 22 4.5 23 5.0 24 4.5 25 5.0 26 7.0 27 8.5 28 12.0 29 20.0 30 24.5 31 26.5 32 26.5 33 28.0 34 33.0 35 45.0 36 101.0 37 214.5 38 267.5 39 245.5 40 267.5 41 300.5 42 252.0 43 228.0 44 242.0 45 233.0 46 194.0 47 162.5 48 150.0 49 101.5 50 79.0 51 73.5 52 63.5 53 54.5 54 52.0 55 65.0 56 73.0 57 60.5 58 47.5 59 44.0 60 41.0 61 28.5 62 14.0 63 15.0 64 20.5 65 19.5 66 12.0 67 5.0 68 3.0 69 3.5 70 3.5 71 2.0 72 1.0 73 0.5 74 1.5 75 1.5 76 0.5 77 0.5 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.22499999999999998 2 0.0 3 0.0 4 0.0 5 0.17500000000000002 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.01 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.11 125-129 0.01 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.1625 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.675000000000004 #Duplication Level Percentage of deduplicated Percentage of total 1 83.17794892582083 51.300000000000004 2 8.593433319821646 10.6 3 2.7563842723956222 5.1 4 1.2971220105391164 3.2 5 0.9728415079043373 3.0 6 0.8512363194162952 3.15 7 0.24321037697608433 1.05 8 0.3242805026347791 1.6 9 0.12160518848804217 0.675 >10 1.6214025131738954 18.375 >50 0.040535062829347386 1.95 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GCGGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTACG 78 1.95 No Hit GTGCTCTGCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTCCTA 43 1.075 No Hit GGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTACGTT 42 1.05 No Hit GGCCAAAATAACCATGAGCAGCCACAATATTATAAGTCTCTTCCTCTTGA 38 0.95 No Hit GCTTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCA 38 0.95 No Hit CTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCATTAAT 28 0.7000000000000001 No Hit GCCAAAATAACCATGAGCAGCCACAATATTATAAGTCTCTTCCTCTTGAC 28 0.7000000000000001 No Hit GTTGAAACTAGCATATTGGAAGATTAATCGGCCAAAATAACCATGAGCAG 26 0.65 No Hit GTTAGCGGAAAAACCTTATCCATTAATAGCGGGAACTTCAAGAGCAGCTA 24 0.6 No Hit CTTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCAT 24 0.6 No Hit GCGGAAAAACCTTATCCATTAATAGCGGGAACTTCAAGAGCAGCTAGATC 24 0.6 No Hit GGATGCATAAGGATGTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAA 22 0.5499999999999999 No Hit GTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTC 21 0.525 No Hit GGTCAATAAGGTAGGGATCATCAAAACACCGAACCATCCGATGTAAAGAC 20 0.5 No Hit GCTCTGCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTCCTAAA 20 0.5 No Hit GCATAAGGATGTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAAAGTA 19 0.475 No Hit GCACGGTTGATGATATCAGCCCAAGTATTAATAACGCGACCTTGACTATC 18 0.44999999999999996 No Hit GCCGAAAACACCAGCTACACCTAACATGTGAAATGGATGCATAAGGATGT 17 0.42500000000000004 No Hit GTTGATGATATCAGCCCAAGTATTAATAACGCGACCTTGACTATCAACTA 15 0.375 No Hit GATGCATAAGGATGTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAAA 15 0.375 No Hit GCCAAGAAGAAGTGTAAAGAACGAGAGTTGTTGAAACTAGCATATTGGAA 15 0.375 No Hit GTGAGCATTACGTTCGTGCATTACTTCCATACCAAGATTAGCACGGTTGA 14 0.35000000000000003 No Hit CCACAATATTATAAGTCTCTTCCTCTTGACCAAATTTGTAACCCTCATTA 14 0.35000000000000003 No Hit GCGGTCAATAAGGTAGGGATCATCAAAACACCGAACCATCCGATGTAAAG 13 0.325 No Hit GGAGGGGCTGCGATGAAGGCGATAATAAATACAGAAGTTGCGGTCAATAA 13 0.325 No Hit GGTTGATGATATCAGCCCAAGTATTAATAACGCGACCTTGACTATCAACT 13 0.325 No Hit GCGACCTTGACTATCAACTACAGATTGGTTGAAATTGAATCCATTTAGGT 13 0.325 No Hit GCTAGTTATCCAGTTGCAGAAGCGACCCCACAGGCTTGTACTTTCGCGTC 13 0.325 No Hit CTCTGCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTCCTAAAG 13 0.325 No Hit GGCGATAATAAATACAGAAGTTGCGGTCAATAAGGTAGGGATCATCAAAA 12 0.3 No Hit CCCAAGTATTAATAACGCGACCTTGACTATCAACTACAGATTGGTTGAAA 12 0.3 No Hit CCAGATTCCTACTACAGGCCAAGCAGCCAAGAAGAAGTGTAAAGAACGAG 12 0.3 No Hit GAGTTGTTGAAACTAGCATATTGGAAGATTAATCGGCCAAAATAACCATG 12 0.3 No Hit GTCGCAGCTGCAACAGGAGCTGAATATGCAACAGCAATCCAAGGGCGCAT 11 0.27499999999999997 No Hit CTCTTGACCAAATTTGTAACCCTCATTAGCAGATTCATTTTCAGTAGTTT 11 0.27499999999999997 No Hit GCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTCCTAAAGGCAT 11 0.27499999999999997 No Hit GGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTACGTTC 11 0.27499999999999997 No Hit GTTGCGGTCAATAAGGTAGGGATCATCAAAACACCGAACCATCCGATGTA 10 0.25 No Hit GTAAAGAACGAGAGTTGTTGAAACTAGCATATTGGAAGATTAATCGGCCA 10 0.25 No Hit GGCCAAGCAGCCAAGAAGAAGTGTAAAGAACGAGAGTTGTTGAAACTAGC 10 0.25 No Hit GGGGCTGCGATGAAGGCGATAATAAATACAGAAGTTGCGGTCAATAAGGT 10 0.25 No Hit CCCTCATTAGCAGATTCATTTTCAGTAGTTTCCCTGATCAAACTAGAGGT 9 0.22499999999999998 No Hit CTCATAAGGACCACCATTGTATAACCATTCATCAACGGATGCAGCTTCCC 9 0.22499999999999998 No Hit CATCAATATCTACTGGAGGGGCTGCGATGAAGGCGATAATAAATACAGAA 9 0.22499999999999998 No Hit CGGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTACGT 8 0.2 No Hit CCACCATTGTATAACCATTCATCAACGGATGCAGCTTCCCAAATTGGGTA 8 0.2 No Hit GTAGGGATCATCAAAACACCGAACCATCCGATGTAAAGACGATTTTCAGT 8 0.2 No Hit GGGAATTCGTAGATCCTCCAGACGTAGAGCACGTAGGGCTTTGAAACCAA 8 0.2 No Hit AGCGGAAAAACCTTATCCATTAATAGCGGGAACTTCAAGAGCAGCTAGAT 8 0.2 No Hit GCTAGATCTAGAGGGAAGTTGTGAGCATTACGTTCGTGCATTACTTCCAT 8 0.2 No Hit GTGAAATGGATGCATAAGGATGTTGTGCTCTGCCTGGAATACAATCATAA 8 0.2 No Hit CTCGCGAATACCATCAATATCTACTGGAGGGGCTGCGATGAAGGCGATAA 8 0.2 No Hit GGAAGATTAATCGGCCAAAATAACCATGAGCAGCCACAATATTATAAGTC 7 0.17500000000000002 No Hit GGAAAAACCTTATCCATTAATAGCGGGAACTTCAAGAGCAGCTAGATCTA 7 0.17500000000000002 No Hit TTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCATT 7 0.17500000000000002 No Hit ACGCGACCTTGACTATCAACTACAGATTGGTTGAAATTGAATCCATTTAG 7 0.17500000000000002 No Hit GCATACCCAAACGGAAACTAAGTTCCCACTCACGACCCATATAACAAGCT 7 0.17500000000000002 No Hit GTCCATGTACCAGTAGAAGATTCGGCAGCTACTGCAGCCCCTGCTTCTTC 7 0.17500000000000002 No Hit CGCGAATACCATCAATATCTACTGGAGGGGCTGCGATGAAGGCGATAATA 6 0.15 No Hit GTGCAATCCGATTGCCGCAGAAGTAGGAATAATAGCACCAGAGATAATAT 6 0.15 No Hit ACCAGCTACACCTAACATGTGAAATGGATGCATAAGGATGTTGTGCTCTG 6 0.15 No Hit GCGATAATAAATACAGAAGTTGCGGTCAATAAGGTAGGGATCATCAAAAC 6 0.15 No Hit CTGCGATGAAGGCGATAATAAATACAGAAGTTGCGGTCAATAAGGTAGGG 6 0.15 No Hit CCTCAGCCTACGGGGTATTAGCAACCGTTTCCAGTTGTTGTTCCCCTCCC 6 0.15 No Hit ACCAGATATTCCTAAAGGCATACCATCAGAGAAGCTTCCTTGACCAATAG 6 0.15 No Hit GCCGCAGAAGTAGGAATAATAGCACCAGAGATAATATTGTTTCCATAAAG 6 0.15 No Hit GGCATGATGACTTGGCCTCATCCTCTCCTTCCTCCGGCTTAACACCGGCG 6 0.15 No Hit CAAGTATTAATAACGCGACCTTGACTATCAACTACAGATTGGTTGAAATT 6 0.15 No Hit TGATGATATCAGCCCAAGTATTAATAACGCGACCTTGACTATCAACTACA 6 0.15 No Hit GGGAAGTTGTGAGCATTACGTTCGTGCATTACTTCCATACCAAGATTAGC 6 0.15 No Hit GTTGTTGAAACTAGCATATTGGAAGATTAATCGGCCAAAATAACCATGAG 6 0.15 No Hit GCTGCGATGAAGGCGATAATAAATACAGAAGTTGCGGTCAATAAGGTAGG 6 0.15 No Hit GGCCCATATAACAAGCTACACCAAGTAAGAAGTGTAGAACAATTAGCTCA 6 0.15 No Hit GTGCTAGTTATCCAGTTGCAGAAGCGACCCCACAGGCTTGTACTTTCGCG 6 0.15 No Hit GCCCAAGTATTAATAACGCGACCTTGACTATCAACTACAGATTGGTTGAA 6 0.15 No Hit GTAGGAATAATAGCACCAGAGATAATATTGTTTCCATAAAGTAAAGAACC 6 0.15 No Hit GGAATAATAGCACCAGAGATAATATTGTTTCCATAAAGTAAAGAACCAGA 6 0.15 No Hit CGCGACCTTGACTATCAACTACAGATTGGTTGAAATTGAATCCATTTAGG 6 0.15 No Hit GTCAATAAGGTAGGGATCATCAAAACACCGAACCATCCGATGTAAAGACG 6 0.15 No Hit GCGATGAAGGCGATAATAAATACAGAAGTTGCGGTCAATAAGGTAGGGAT 5 0.125 No Hit CTTGACTATCAACTACAGATTGGTTGAAATTGAATCCATTTAGGTTGAAC 5 0.125 No Hit GCAGAAGTAGGAATAATAGCACCAGAGATAATATTGTTTCCATAAAGTAA 5 0.125 No Hit GCATAGCACTGAATAGGGAACCGCCGAAAACACCAGCTACACCTAACATG 5 0.125 No Hit GTCTCTTCCTCTTGACCAAATTTGTAACCCTCATTAGCAGATTCATTTTC 5 0.125 No Hit GAACCAGATTCCTACTACAGGCCAAGCAGCCAAGAAGAAGTGTAAAGAAC 5 0.125 No Hit AACGAGAGTTGTTGAAACTAGCATATTGGAAGATTAATCGGCCAAAATAA 5 0.125 No Hit GCACTGAATAGGGAACCGCCGAAAACACCAGCTACACCTAACATGTGAAA 5 0.125 No Hit ATAAGGATGTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAAAGTACC 5 0.125 No Hit GGATCATCAAAACACCGAACCATCCGATGTAAAGACGATTTTCAGTGCTA 5 0.125 No Hit GCATATTGGAAGATTAATCGGCCAAAATAACCATGAGCAGCCACAATATT 5 0.125 No Hit AGGAATAATAGCACCAGAGATAATATTGTTTCCATAAAGTAAAGAACCAG 5 0.125 No Hit CGGAAAAACCTTATCCATTAATAGCGGGAACTTCAAGAGCAGCTAGATCT 5 0.125 No Hit GGAAGTTGTGAGCATTACGTTCGTGCATTACTTCCATACCAAGATTAGCA 5 0.125 No Hit CATCAGAGAAGCTTCCTTGACCAATAGGGTAAATCAAGAAAACAGCAGTC 5 0.125 No Hit GCTCCTACTTTTTCATGTTTCCAATCCGATCCCTCCGATTACTATAGAGA 5 0.125 No Hit CAAAGATTTCGGTCAGAGCTGGCATATGCCAAACATGAATACCACCTGAA 5 0.125 No Hit GTGACGGGCGGTGTGTACAAGGCCCGGGAACGGATTCACCGCCGTATGGC 5 0.125 No Hit ATCAATATCTACTGGAGGGGCTGCGATGAAGGCGATAATAAATACAGAAG 5 0.125 No Hit CTCATTAGCAGATTCATTTTCAGTAGTTTCCCTGATCAAACTAGAGGTTA 5 0.125 No Hit CTCCACGCGGCATTGCTCCGTCAGGCTTTCGCCCATTGCGGAAAATTCCC 5 0.125 No Hit GTTCGTGCATTACTTCCATACCAAGATTAGCACGGTTGATGATATCAGCC 5 0.125 No Hit CTGGAATACAATCATAAAGTTGAAAGTACCAGATATTCCTAAAGGCATAC 5 0.125 No Hit GACCCATATAACAAGCTACACCAAGTAAGAAGTGTAGAACAATTAGCTCA 5 0.125 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0125 0.0 0.0 0.0 0.0 58-59 0.025 0.0 0.0 0.0 0.0 60-61 0.025 0.0 0.0 0.0 0.0 62-63 0.025 0.0 0.0 0.0 0.0 64-65 0.037500000000000006 0.0 0.0 0.0 0.0 66-67 0.05 0.0 0.0 0.0 0.0 68-69 0.05 0.0 0.0 0.0 0.0 70-71 0.075 0.0 0.0 0.0 0.0 72-73 0.125 0.0 0.0 0.0 0.0 74-75 0.175 0.0 0.0 0.0 0.0 76-77 0.2625 0.0 0.0 0.0 0.0 78-79 0.3625 0.0 0.0 0.0 0.0 80-81 0.4375 0.0 0.0 0.0 0.0 82-83 0.4875 0.0 0.0 0.0 0.0 84-85 0.55 0.0 0.0 0.0 0.0 86-87 0.6625000000000001 0.0 0.0 0.0 0.0 88-89 0.7124999999999999 0.0 0.0 0.0 0.0 90-91 0.8 0.0 0.0 0.0 0.0 92-93 0.8875 0.0 0.0 0.0 0.0 94-95 1.0 0.0 0.0 0.0 0.0 96-97 1.25 0.0 0.0 0.0 0.0 98-99 1.4 0.0 0.0 0.0 0.0 100-101 1.5125 0.0 0.0 0.0 0.0 102-103 1.725 0.0 0.0 0.0 0.0 104-105 2.0125 0.0 0.0 0.0 0.0 106-107 2.325 0.0 0.0 0.0 0.0 108-109 2.6500000000000004 0.0 0.0 0.0 0.0 110-111 2.875 0.0 0.0 0.0 0.0 112-113 3.2 0.0 0.0 0.0 0.0 114-115 3.7249999999999996 0.0 0.0 0.0 0.0 116-117 4.05 0.0 0.0 0.0 0.0 118-119 4.375 0.0 0.0 0.0 0.0 120-121 4.675000000000001 0.0 0.0 0.0 0.0 122-123 5.050000000000001 0.0 0.0 0.0 0.0 124-125 5.5 0.0 0.0 0.0 0.0 126-127 5.925 0.0 0.0 0.0 0.0 128-129 6.4125 0.0 0.0 0.0 0.0 130-131 6.9375 0.0 0.0 0.0 0.0 132-133 7.300000000000001 0.0 0.0 0.0 0.0 134-135 7.625 0.0 0.0 0.0 0.0 136-137 8.1 0.0 0.0 0.0 0.0 138-139 8.5625 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CAAAATA 25 8.7342097E-4 86.9475 3 AAAATAA 30 0.0018016598 72.45625 4 >>END_MODULE SRR6941542 read2 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR6941542_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 33.76125 35.0 35.0 35.0 32.0 35.0 2 33.9805 35.0 35.0 35.0 32.0 35.0 3 34.26975 35.0 35.0 35.0 33.0 35.0 4 33.9415 35.0 35.0 35.0 32.0 35.0 5 33.9945 35.0 34.0 35.0 32.0 35.0 6 38.878 40.0 39.0 40.0 38.0 40.0 7 38.79325 40.0 39.0 40.0 37.0 40.0 8 39.1285 40.0 40.0 40.0 39.0 40.0 9 39.2495 40.0 40.0 40.0 39.0 40.0 10-14 39.165949999999995 40.0 40.0 40.0 38.8 40.0 15-19 39.23245 40.0 40.0 40.0 39.0 40.0 20-24 39.2227 40.0 40.0 40.0 39.0 40.0 25-29 39.20285 40.0 40.0 40.0 39.0 40.0 30-34 39.1353 40.0 40.0 40.0 38.8 40.0 35-39 39.013 40.0 39.8 40.0 38.2 40.0 40-44 39.1053 40.0 40.0 40.0 38.8 40.0 45-49 39.08135 40.0 40.0 40.0 38.8 40.0 50-54 38.851150000000004 40.0 39.2 40.0 37.6 40.0 55-59 38.8086 40.0 39.2 40.0 37.8 40.0 60-64 38.789699999999996 40.0 39.2 40.0 37.2 40.0 65-69 38.8108 40.0 39.2 40.0 37.4 40.0 70-74 38.678200000000004 40.0 39.0 40.0 36.6 40.0 75-79 38.701750000000004 40.0 39.0 40.0 37.0 40.0 80-84 38.728699999999996 40.0 39.0 40.0 37.0 40.0 85-89 38.8331 40.0 39.0 40.0 37.4 40.0 90-94 38.771950000000004 40.0 39.0 40.0 37.2 40.0 95-99 38.50535000000001 40.0 39.0 40.0 36.2 40.0 100-104 37.1728 38.6 37.6 39.2 33.6 39.4 105-109 38.5171 40.0 39.0 40.0 36.4 40.0 110-114 38.614999999999995 40.0 39.0 40.0 36.8 40.0 115-119 38.435300000000005 40.0 39.0 40.0 36.0 40.0 120-124 38.322050000000004 40.0 39.0 40.0 35.6 40.0 125-129 38.46124999999999 40.0 39.0 40.0 36.2 40.0 130-134 38.2876 40.0 39.0 40.0 36.0 40.0 135-139 38.25484999999999 40.0 39.0 40.0 36.2 40.0 140-144 37.849149999999995 40.0 39.0 40.0 35.2 40.0 145-149 37.47095 40.0 38.8 40.0 33.8 40.0 150-151 34.51575 38.0 34.5 39.5 24.5 40.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 2.0 12 1.0 13 4.0 14 0.0 15 0.0 16 0.0 17 4.0 18 1.0 19 0.0 20 3.0 21 5.0 22 7.0 23 9.0 24 7.0 25 13.0 26 11.0 27 16.0 28 17.0 29 24.0 30 24.0 31 40.0 32 41.0 33 32.0 34 60.0 35 73.0 36 112.0 37 167.0 38 438.0 39 2887.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 49.418310571573095 20.763783510369247 11.229135053110774 18.588770864946888 2 23.587038432554635 21.50213514192414 35.995980909319265 18.914845516201957 3 19.975 24.625 39.975 15.425 4 23.025000000000002 30.55 27.224999999999998 19.2 5 25.4 32.300000000000004 25.374999999999996 16.925 6 20.974999999999998 34.075 27.750000000000004 17.2 7 18.975 21.099999999999998 41.475 18.45 8 20.375 24.025 30.75 24.85 9 20.674999999999997 21.5 36.15 21.675 10-14 23.47 26.0 30.875000000000004 19.655 15-19 23.244999999999997 24.695 32.235 19.825 20-24 23.28 24.69 32.800000000000004 19.23 25-29 22.830000000000002 25.535000000000004 31.019999999999996 20.615 30-34 24.34 24.95 31.545 19.165 35-39 23.93 24.505 31.805 19.759999999999998 40-44 23.765 25.509999999999998 30.630000000000003 20.095 45-49 22.685 26.61 30.654999999999998 20.05 50-54 23.765 25.16 31.485000000000003 19.59 55-59 22.81 26.83 29.625 20.735 60-64 22.795 24.725 31.595000000000002 20.885 65-69 23.35 25.074999999999996 31.045 20.53 70-74 23.669999999999998 25.56 30.464999999999996 20.305 75-79 23.419999999999998 24.82 31.2 20.560000000000002 80-84 23.73 24.19 32.765 19.314999999999998 85-89 24.884999999999998 25.445 29.709999999999997 19.96 90-94 23.765 25.505 29.830000000000002 20.9 95-99 23.06 25.2 30.740000000000002 21.0 100-104 23.735 25.259999999999998 30.95 20.055 105-109 25.115 24.240000000000002 30.64 20.005 110-114 24.095 25.455 30.275000000000002 20.175 115-119 24.709999999999997 24.815 31.025000000000002 19.45 120-124 24.05 26.135 29.285 20.53 125-129 24.145 25.835 29.39 20.630000000000003 130-134 24.21 26.105 29.38 20.305 135-139 23.79 26.075 30.709999999999997 19.425 140-144 24.815 25.665 30.735 18.785 145-149 24.375 24.985 31.130000000000003 19.509999999999998 150-151 23.233967935871743 25.150300601202403 32.37725450901804 19.238476953907817 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.5 15 0.5 16 0.0 17 0.0 18 1.0 19 1.0 20 1.0 21 3.5 22 3.5 23 4.0 24 9.0 25 10.5 26 11.0 27 13.0 28 16.0 29 24.5 30 35.5 31 34.5 32 33.5 33 53.0 34 85.5 35 91.5 36 102.5 37 168.5 38 201.0 39 210.0 40 236.0 41 249.5 42 229.5 43 245.5 44 277.0 45 225.5 46 192.0 47 179.5 48 138.0 49 92.5 50 78.0 51 81.0 52 57.0 53 49.0 54 64.5 55 73.0 56 70.5 57 52.5 58 39.0 59 38.5 60 40.0 61 41.0 62 29.5 63 21.5 64 20.5 65 17.5 66 14.5 67 10.0 68 8.0 69 4.5 70 3.5 71 2.5 72 1.0 73 0.5 74 0.0 75 1.0 76 1.0 77 0.0 78 0.5 79 0.5 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 1.15 2 0.475 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.2 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 64.5 #Duplication Level Percentage of deduplicated Percentage of total 1 81.89922480620156 52.825 2 8.837209302325581 11.4 3 2.9069767441860463 5.625 4 2.0155038759689923 5.2 5 1.4728682170542635 4.75 6 0.5813953488372093 2.25 7 0.46511627906976744 2.1 8 0.23255813953488372 1.2 9 0.1937984496124031 1.125 >10 1.3953488372093024 13.525 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GCACAACATCCTTATGCATCCATTTCACATGTTAGGTGTAGCTGGTGTTT 30 0.75 No Hit ATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAA 27 0.675 No Hit GTGGGAACTTAGTTTCCGTTTGGGTATGCGCCCTTGGATTGCTGTTGCAT 24 0.6 No Hit ATTATCTATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAAT 23 0.575 No Hit GCTAGTTTCAACAACTCTCGTTCTTTACACTTCTTCTTGGCTGCTTGGCC 22 0.5499999999999999 No Hit GCTTGTTATATGGGTCGTGAGTGGGAACTTAGTTTCCGTTTGGGTATGCG 21 0.525 No Hit GTTATTTTGGCCGATTAATCTTCCAATATGCTAGTTTCAACAACTCTCGT 21 0.525 No Hit TATCTATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTT 19 0.475 No Hit GGGAAACTACTGAAAATGAATCTGCTAATGAGGGTTACAAATTTGGTCAA 18 0.44999999999999996 No Hit ATCTATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTG 18 0.44999999999999996 No Hit GTTTCAACAACTCTCGTTCTTTACACTTCTTCTTGGCTGCTTGGCCTGTA 17 0.42500000000000004 No Hit GTTAGGTGTAGCTGGTGTTTTCGGCGGTTCCCTATTCAGTGCTATGCATG 16 0.4 No Hit GGGAACTTAGTTTCCGTTTGGGTATGCGCCCTTGGATTGCTGTTGCATAT 15 0.375 No Hit GTTCAACCTAAATGGATTCAATTTCAACCAATCTGTAGTTGATAGTCAAG 15 0.375 No Hit GCTGCGACTGCTGTTTTCTTGATTTACCCTATTGGTCAAGGAAGCTTCTC 14 0.35000000000000003 No Hit GCTGCATCCGTTGATGAATGGTTATACAATGGTGGTCCTTATGAGCTAAT 14 0.35000000000000003 No Hit GTCAAGGTCGCGTTATTAATACTTGGGCTGATATCATCAACCGTGCTAAT 14 0.35000000000000003 No Hit GTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGATCTT 13 0.325 No Hit ATTTGGTCAAGAGGAAGAGACTTATAATATTGTGGCTGCTCATGGTTATT 13 0.325 No Hit GTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGATCTTACCATGAC 13 0.325 No Hit GCTAATTGTTCTACACTTCTTACTTGGTGTAGCTTGTTATATGGGTCGTG 12 0.3 No Hit GTTATACAATGGTGGTCCTTATGAGCTAATTGTTCTACACTTCTTACTTG 12 0.3 No Hit GGAAGCTTCTCTGATGGTATGCCTTTAGGAATATCTGGTACTTTCAACTT 12 0.3 No Hit GTTTCTGGTTCTTTACTTTATGGAAACAATATTATCTCTGGTGCTATTAT 12 0.3 No Hit AGCACAACATCCTTATGCATCCATTTCACATGTTAGGTGTAGCTGGTGTT 11 0.27499999999999997 No Hit GGTCGCTTCTGCAACTGGATAACTAGCACTGAAAATCGTCTTTACATCGG 11 0.27499999999999997 No Hit CTATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAA 11 0.27499999999999997 No Hit GTAGCTTGTTATATGGGTCGTGAGTGGGAACTTAGTTTCCGTTTGGGTAT 11 0.27499999999999997 No Hit GTACAAGCCTGTGGGGTCGCTTCTGCAACTGGATAACTAGCACTGAAAAT 11 0.27499999999999997 No Hit GTTTGATCAGGGAAACTACTGAAAATGAATCTGCTAATGAGGGTTACAAA 11 0.27499999999999997 No Hit CCAAGATCTTACCATGACTGCAATTTTAGAGAGACGCGAAAGTACAAGCC 10 0.25 No Hit GTTCGATCCTGGCTCAGGATGAACGCTGGCGGCATGCTTAACACATGCAA 10 0.25 No Hit GTTGCATATTCAGCTCCTGTTGCAGCTGCGACTGCTGTTTTCTTGATTTA 10 0.25 No Hit CGGATGGTTCGGTGTTTTGATGATCCCTACCTTATTGACCGCAACTTCTG 10 0.25 No Hit GAAGAGACTTATAATATTGTGGCTGCTCATGGTTATTTTGGCCGATTAAT 10 0.25 No Hit GGTACTTTCAACTTTATGATTGTATTCCAGGCAGAGCACAACATCCTTAT 10 0.25 No Hit GTTCCTTGGTAACCTCTAGTTTGATCAGGGAAACTACTGAAAATGAATCT 9 0.22499999999999998 No Hit CTTTAGGAATATCTGGTACTTTCAACTTTATGATTGTATTCCAGGCAGAG 9 0.22499999999999998 No Hit GTTATATGGGTCGTGAGTGGGAACTTAGTTTCCGTTTGGGTATGCGCCCT 9 0.22499999999999998 No Hit AGGGAAACTACTGAAAATGAATCTGCTAATGAGGGTTACAAATTTGGTCA 9 0.22499999999999998 No Hit GTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGATCTTACCATGACTG 9 0.22499999999999998 No Hit GCTCATGGTTATTTTGGCCGATTAATCTTCCAATATGCTAGTTTCAACAA 8 0.2 No Hit GCTTGGGAGTCCTTGCAATTTGAATAAACCAAGATCTTACCATGACTGCA 8 0.2 No Hit GCTAATGAGGGTTACAAATTTGGTCAAGAGGAAGAGACTTATAATATTGT 8 0.2 No Hit GCTGTTTTCTTGATTTACCCTATTGGTCAAGGAAGCTTCTCTGATGGTAT 8 0.2 No Hit GCATGGTTCCTTGGTAACCTCTAGTTTGATCAGGGAAACTACTGAAAATG 8 0.2 No Hit GTAGGAATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCT 8 0.2 No Hit GGAAGAGACTTATAATATTGTGGCTGCTCATGGTTATTTTGGCCGATTAA 7 0.17500000000000002 No Hit GTTTGGGTATGCGCCCTTGGATTGCTGTTGCATATTCAGCTCCTGTTGCA 7 0.17500000000000002 No Hit ATCGGATTGCACTTTTACCCAATTTGGGAAGCTGCATCCGTTGATGAATG 7 0.17500000000000002 No Hit CAACTCTCGTTCTTTACACTTCTTCTTGGCTGCTTGGCCTGTAGTAGGAA 7 0.17500000000000002 No Hit GATGGTATTCGCGAGCCTGTTTCTGGTTCTTTACTTTATGGAAACAATAT 7 0.17500000000000002 No Hit GGAATATCTGGTACTTTCAACTTTATGATTGTATTCCAGGCAGAGCACAA 7 0.17500000000000002 No Hit GTTTTGATGATCCCTACCTTATTGACCGCAACTTCTGTATTTATTATCGC 7 0.17500000000000002 No Hit ATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATA 7 0.17500000000000002 No Hit GCCTTTAGGAATATCTGGTACTTTCAACTTTATGATTGTATTCCAGGCAG 7 0.17500000000000002 No Hit TCTATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGA 7 0.17500000000000002 No Hit CAGAGCACAACATCCTTATGCATCCATTTCACATGTTAGGTGTAGCTGGT 7 0.17500000000000002 No Hit GAATAAACCAAGATCTTACCATGACTGCAATTTTAGAGAGACGCGAAAGT 7 0.17500000000000002 No Hit GTCGCTTCTGCAACTGGATAACTAGCACTGAAAATCGTCTTTACATCGGA 6 0.15 No Hit CTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGAT 6 0.15 No Hit CCTAAATGGATTCAATTTCAACCAATCTGTAGTTGATAGTCAAGGTCGCG 6 0.15 No Hit GCTCAGTTGGTAGAGCTCCGCTCTTGCAATTGGGTCGTTGCGATTACGGG 6 0.15 No Hit CTCTAGTTTGATCAGGGAAACTACTGAAAATGAATCTGCTAATGAGGGTT 6 0.15 No Hit ATTAGTACTATGGCGTTCAACCTAAATGGATTCAATTTCAACCAATCTGT 6 0.15 No Hit GTGCTATGCATGGTTCCTTGGTAACCTCTAGTTTGATCAGGGAAACTACT 6 0.15 No Hit GTTGATAGTCAAGGTCGCGTTATTAATACTTGGGCTGATATCATCAACCG 6 0.15 No Hit GGAATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCTAAA 6 0.15 No Hit AGTCAAGGTCGCGTTATTAATACTTGGGCTGATATCATCAACCGTGCTAA 6 0.15 No Hit ATTCAGTGCTATGCATGGTTCCTTGGTAACCTCTAGTTTGATCAGGGAAA 6 0.15 No Hit ATTCAGCTCCTGTTGCAGCTGCGACTGCTGTTTTCTTGATTTACCCTATT 6 0.15 No Hit GAGGAAGAGACTTATAATATTGTGGCTGCTCATGGTTATTTTGGCCGATT 6 0.15 No Hit CTTCCAATATGCTAGTTTCAACAACTCTCGTTCTTTACACTTCTTCTTGG 6 0.15 No Hit AGAACCTTACCAGGGCTTGACATGCCGCGAATCCTCTTGAAAGAGAGGGG 6 0.15 No Hit AAGAGACTTATAATATTGTGGCTGCTCATGGTTATTTTGGCCGATTAATC 5 0.125 No Hit GTTTTCGGCGGTTCCCTATTCAGTGCTATGCATGGTTCCTTGGTAACCTC 5 0.125 No Hit CATGGTTATTTTGGCCGATTAATCTTCCAATATGCTAGTTTCAACAACTC 5 0.125 No Hit GCGCCCTTGGATTGCTGTTGCATATTCAGCTCCTGTTGCAGCTGCGACTG 5 0.125 No Hit GGTAACCTCTAGTTTGATCAGGGAAACTACTGAAAATGAATCTGCTAATG 5 0.125 No Hit CCTTATGAGCTAATTGTTCTACACTTCTTACTTGGTGTAGCTTGTTATAT 5 0.125 No Hit CCTGTTGCAGCTGCGACTGCTGTTTTCTTGATTTACCCTATTGGTCAAGG 5 0.125 No Hit GAATCTGCTAATGAGGGTTACAAATTTGGTCAAGAGGAAGAGACTTATAA 5 0.125 No Hit CTGGTACTTTCAACTTTATGATTGTATTCCAGGCAGAGCACAACATCCTT 5 0.125 No Hit GTCGTGAGTGGGAACTTAGTTTCCGTTTGGGTATGCGCCCTTGGATTGCT 5 0.125 No Hit CTCTCGTTCTTTACACTTCTTCTTGGCTGCTTGGCCTGTAGTAGGAATCT 5 0.125 No Hit ATTTCAACCAATCTGTAGTTGATAGTCAAGGTCGCGTTATTAATACTTGG 5 0.125 No Hit TATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAA 5 0.125 No Hit GCGAGAGCTTGTAACCCGAGTGGGGGCTTTAAGGGTGGTGTGGACGTTGC 5 0.125 No Hit GGTTATTTTGGCCGATTAATCTTCCAATATGCTAGTTTCAACAACTCTCG 5 0.125 No Hit GGTATTCGCGAGCCTGTTTCTGGTTCTTTACTTTATGGAAACAATATTAT 5 0.125 No Hit TTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAG 5 0.125 No Hit AAACAATATTATCTCTGGTGCTATTATTCCTACTTCTGCGGCAATCGGAT 5 0.125 No Hit ATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCTAAATGG 5 0.125 No Hit GGATGGTTCGGTGTTTTGATGATCCCTACCTTATTGACCGCAACTTCTGT 5 0.125 No Hit ATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCA 5 0.125 No Hit GTTATTAATACTTGGGCTGATATCATCAACCGTGCTAATCTTGGTATGGA 5 0.125 No Hit CATCAACCGTGCTAATCTTGGTATGGAAGTAATGCACGAACGTAATGCTC 5 0.125 No Hit TGTAGCTGGTGTTTTCGGCGGTTCCCTATTCAGTGCTATGCATGGTTCCT 5 0.125 No Hit AGCTGGTGTTTTCGGCGGTTCCCTATTCAGTGCTATGCATGGTTCCTTGG 5 0.125 No Hit GTGAGAATTCTTAATTCAAGAGTTGTAAGGAGGGACTTATGTCACCACAA 5 0.125 No Hit GTTGATGAATGGTTATACAATGGTGGTCCTTATGAGCTAATTGTTCTACA 5 0.125 No Hit CAGGGAAACTACTGAAAATGAATCTGCTAATGAGGGTTACAAATTTGGTC 5 0.125 No Hit AACATCCTTATGCATCCATTTCACATGTTAGGTGTAGCTGGTGTTTTCGG 5 0.125 No Hit CCTGAACAGACCGCCGGTGTTAAGCCGGAGGAAGGAGAGGATGAGGCCAA 5 0.125 No Hit GCTTTAGGTATTAGTACTATGGCGTTCAACCTAAATGGATTCAATTTCAA 5 0.125 No Hit GAATGGTTATACAATGGTGGTCCTTATGAGCTAATTGTTCTACACTTCTT 5 0.125 No Hit AGTAGATATTGATGGTATTCGCGAGCCTGTTTCTGGTTCTTTACTTTATG 5 0.125 No Hit ATTGATGGTATTCGCGAGCCTGTTTCTGGTTCTTTACTTTATGGAAACAA 5 0.125 No Hit GCTAACTCCAAAAACCCGTCCTCAGTTCGGATTGCAGGCTGCAACTCGCC 5 0.125 No Hit GTTTAGTGGTAAAAGTGTGATTCGTTCTATTAATAACTGAATTTAAAATG 5 0.125 No Hit TGATAGTCAAGGTCGCGTTATTAATACTTGGGCTGATATCATCAACCGTG 5 0.125 No Hit AGCTTGTTATATGGGTCGTGAGTGGGAACTTAGTTTCCGTTTGGGTATGC 5 0.125 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0125 0.0 0.0 0.0 0.0 58-59 0.025 0.0 0.0 0.0 0.0 60-61 0.025 0.0 0.0 0.0 0.0 62-63 0.025 0.0 0.0 0.0 0.0 64-65 0.037500000000000006 0.0 0.0 0.0 0.0 66-67 0.05 0.0 0.0 0.0 0.0 68-69 0.05 0.0 0.0 0.0 0.0 70-71 0.075 0.0 0.0 0.0 0.0 72-73 0.1375 0.0 0.0 0.0 0.0 74-75 0.2 0.0 0.0 0.0 0.0 76-77 0.3125 0.0 0.0 0.0 0.0 78-79 0.4125 0.0 0.0 0.0 0.0 80-81 0.48750000000000004 0.0 0.0 0.0 0.0 82-83 0.5375000000000001 0.0 0.0 0.0 0.0 84-85 0.6 0.0 0.0 0.0 0.0 86-87 0.7124999999999999 0.0 0.0 0.0 0.0 88-89 0.7625 0.0 0.0 0.0 0.0 90-91 0.85 0.0 0.0 0.0 0.0 92-93 0.9375 0.0 0.0 0.0 0.0 94-95 1.0375 0.0 0.0 0.0 0.0 96-97 1.25 0.0 0.0 0.0 0.0 98-99 1.375 0.0 0.0 0.0 0.0 100-101 1.4875 0.0 0.0 0.0 0.0 102-103 1.7 0.0 0.0 0.0 0.0 104-105 1.9874999999999998 0.0 0.0 0.0 0.0 106-107 2.3 0.0 0.0 0.0 0.0 108-109 2.625 0.0 0.0 0.0 0.0 110-111 2.8625 0.0 0.0 0.0 0.0 112-113 3.2 0.0 0.0 0.0 0.0 114-115 3.7249999999999996 0.0 0.0 0.0 0.0 116-117 4.0375 0.0 0.0 0.0 0.0 118-119 4.325 0.0 0.0 0.0 0.0 120-121 4.625 0.0 0.0 0.0 0.0 122-123 5.0 0.0 0.0 0.0 0.0 124-125 5.449999999999999 0.0 0.0 0.0 0.0 126-127 5.85 0.0 0.0 0.0 0.0 128-129 6.3375 0.0 0.0 0.0 0.0 130-131 6.8875 0.0 0.0 0.0 0.0 132-133 7.25 0.0 0.0 0.0 0.0 134-135 7.574999999999999 0.0 0.0 0.0 0.0 136-137 8.075 0.0 0.0 0.0 0.0 138-139 8.537500000000001 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TTATTTT 35 0.003150469 62.918625 2 TTTGGCC 35 0.0033146844 62.13214 6 ATTTTGG 35 0.0033146844 62.13214 4 TTTTGGC 40 0.0056251725 54.365623 5 GGCCGAT 40 0.0056251725 54.365623 9 TATTTTG 40 0.0056251725 54.365623 3 GTTATTT 45 0.008520494 48.93671 1 TTGGCCG 45 0.008963385 48.325 7 ATTTCAA 45 0.008963385 48.325 145 TGGCCGA 45 0.008963385 48.325 8 >>END_MODULE Read 1081907 spots for SRR6941542.sra Written 1081907 spots for SRR6941542.sra Read 1081907 spots for SRR6941542.sra Written 1081907 spots for SRR6941542.sra Read 1081907 spots for SRR6941542.sra Written 1081907 spots for SRR6941542.sra Read 1081907 spots for SRR6941542.sra Written 1081907 spots for SRR6941542.sra Read 1081907 spots for SRR6941542.sra Written 1081907 spots for SRR6941542.sra Read 1081907 spots for SRR6941542.sra Written 1081907 spots for SRR6941542.sra Read 1081907 spots for SRR6941542.sra Written 1081907 spots for SRR6941542.sra Read 1081907 spots for SRR6941542.sra Written 1081907 spots for SRR6941542.sra Read 1081907 spots for SRR6941542.sra Written 1081907 spots for SRR6941542.sra Read 1081907 spots for SRR6941542.sra Written 1081907 spots for SRR6941542.sra Read 1081907 spots for SRR6941542.sra Written 1081907 spots for SRR6941542.sra Read 1081907 spots for SRR6941542.sra Written 1081907 spots for SRR6941542.sra Read 1081907 spots for SRR6941542.sra Written 1081907 spots for SRR6941542.sra Read 1081907 spots for SRR6941542.sra Written 1081907 spots for SRR6941542.sra Read 1081907 spots for SRR6941542.sra Written 1081907 spots for SRR6941542.sra Read 1081907 spots for SRR6941542.sra Written 1081907 spots for SRR6941542.sra Read 1081907 spots for SRR6941542.sra Written 1081907 spots for SRR6941542.sra Read 1081907 spots for SRR6941542.sra Written 1081907 spots for SRR6941542.sra Read 1081925 spots for SRR6941542.sra Written 1081925 spots for SRR6941542.sra Read 1081907 spots for SRR6941542.sra Written 1081907 spots for SRR6941542.sra SRR ids: ['SRR6941542.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_8gzqia09 SRR6941542.sra spots: 21638158 blocks: [[1, 1081907], [1081908, 2163814], [2163815, 3245721], [3245722, 4327628], [4327629, 5409535], [5409536, 6491442], [6491443, 7573349], [7573350, 8655256], [8655257, 9737163], [9737164, 10819070], [10819071, 11900977], [11900978, 12982884], [12982885, 14064791], [14064792, 15146698], [15146699, 16228605], [16228606, 17310512], [17310513, 18392419], [18392420, 19474326], [19474327, 20556233], [20556234, 21638158]] SRR6941542 file size 7310761 SRR6941542 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR6941542 SRR6941542_1.fastq SRR6941542_2.fastq Input file: SRR6941542_1.fastq Paired file: SRR6941542_2.fastq trimmed: SRR6941542-trimmed-pair1.fastq, SRR6941542-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Fri Dec 6 10:49:05 2024 >> started Fri Dec 6 10:49:29 2024 >> done (23.141s) 21638158 read pairs processed; of these: 9588 ( 0.04%) short read pairs filtered out after trimming by size control 11764 ( 0.05%) empty read pairs filtered out after trimming by size control 21616806 (99.90%) read pairs available; of these: 4246315 (19.64%) trimmed read pairs available after processing 17370491 (80.36%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 2 0.00% 19 0 0.00% 20 2 0.00% 21 2 0.00% 22 2 0.00% 23 4 0.00% 24 5 0.00% 25 8 0.00% 26 8 0.00% 27 12 0.00% 28 12 0.00% 29 8 0.00% 30 13 0.00% 31 14 0.00% 32 21 0.00% 33 25 0.00% 34 23 0.00% 35 29 0.00% 36 40 0.00% 37 40 0.00% 38 44 0.00% 39 80 0.00% 40 86 0.00% 41 89 0.00% 42 116 0.00% 43 106 0.00% 44 134 0.00% 45 144 0.00% 46 172 0.00% 47 190 0.00% 48 213 0.00% 49 285 0.00% 50 307 0.00% 51 370 0.00% 52 452 0.00% 53 461 0.00% 54 511 0.00% 55 580 0.00% 56 585 0.00% 57 728 0.00% 58 815 0.00% 59 905 0.00% 60 1115 0.01% 61 1181 0.01% 62 1516 0.01% 63 1613 0.01% 64 1883 0.01% 65 2027 0.01% 66 2259 0.01% 67 2274 0.01% 68 2854 0.01% 69 3260 0.02% 70 3555 0.02% 71 4154 0.02% 72 4700 0.02% 73 5297 0.02% 74 5071 0.02% 75 5891 0.03% 76 5940 0.03% 77 6732 0.03% 78 7027 0.03% 79 7485 0.03% 80 8529 0.04% 81 9340 0.04% 82 10669 0.05% 83 10630 0.05% 84 11512 0.05% 85 13613 0.06% 86 14035 0.06% 87 14736 0.07% 88 16413 0.08% 89 16755 0.08% 90 19012 0.09% 91 18999 0.09% 92 22152 0.10% 93 22079 0.10% 94 23268 0.11% 95 24638 0.11% 96 23502 0.11% 97 22907 0.11% 98 23093 0.11% 99 23888 0.11% 100 24698 0.11% 101 27321 0.13% 102 29917 0.14% 103 29713 0.14% 104 30922 0.14% 105 32630 0.15% 106 32361 0.15% 107 32311 0.15% 108 32979 0.15% 109 35182 0.16% 110 36422 0.17% 111 39669 0.18% 112 41335 0.19% 113 39827 0.18% 114 43351 0.20% 115 39351 0.18% 116 40500 0.19% 117 38769 0.18% 118 39151 0.18% 119 39451 0.18% 120 40388 0.19% 121 41767 0.19% 122 49653 0.23% 123 49376 0.23% 124 51764 0.24% 125 51629 0.24% 126 48608 0.22% 127 48581 0.22% 128 48679 0.23% 129 52549 0.24% 130 52053 0.24% 131 56111 0.26% 132 56412 0.26% 133 50149 0.23% 134 57841 0.27% 135 55312 0.26% 136 59329 0.27% 137 56945 0.26% 138 62940 0.29% 139 62913 0.29% 140 60322 0.28% 141 72098 0.33% 142 63606 0.29% 143 68447 0.32% 144 67179 0.31% 145 77674 0.36% 146 83650 0.39% 147 89245 0.41% 148 112011 0.52% 149 162889 0.75% 150 1197088 5.54% 151 17370491 80.36% 21616806 reads passed initial QC criterion=sequence-density sequence-density=0.31 sequence-density-rank=1 fanout-score=1.97 fanout-score-rank=32 prefix-density=0.31 prefix-fanout=2.0 sequence=AATATACCCAATG criterion=fanout-score sequence-density=0.02 sequence-density-rank=34 fanout-score=57.12 fanout-score-rank=1 prefix-density=0.19 prefix-fanout=4.9 sequence=ATAAAAAAAAGGGGGGGTAAGGACCCGCTAAGCTCCTACTTTTTCATGTTTCCAATCCGATCCCTCCGATTACTATAGAGATGAACCCAATCCAGAATATGAACCATAAAAGAAAACACCTACTAAACCAATCACAAGAATACCAGTTACCGTACCTATCAGCCAAAGAGGAATTCTTCCAGTAGTATCGGCCATTTCCCCTACTTTCCTCCACATTTTATCAAGTGGTCATGCTAGAGACAAAAACAGTCATGGATAGTTATGTTATAAGGATGGTATCCTTCCAAATGGGATAAGAGAGTTCTTACTACTCTCTTCTTTTCTCTCAATTAAAGAAGTAATTGGAAAACAAAACAGCAAGTACAAAAATGAGTAATAAACCCCAGTATAGACTGGTACGATTCAATTCAACATTTTGTTCATTCGGGTTTGATTGTGTCATAGTTCTATAGTTGGAATTTAGTTTATCGTTGGATGAACTGCATTGCTGATATTGATCCCAAGAAAAAAA criterion=sequence-density sequence-density=0.32 sequence-density-rank=1 fanout-score=2.28 fanout-score-rank=25 prefix-density=0.33 prefix-fanout=2.2 sequence=ACTGCCCGCTTCCAACGGTGGAAGGATAACGGGCCGCTGCA criterion=fanout-score sequence-density=0.01 sequence-density-rank=38 fanout-score=17.63 fanout-score-rank=1 prefix-density=0.07 prefix-fanout=1.5 sequence=GATATAGCTCAGTTGGTAGAGCTCCGCTCTTGCAATTGGGTCGTTGCGATTACGGGTTGGCTGTCTAATTGTCCAGGCGGTAATGATAGTATCTTGTACCTGAACCGGTGGCTCACTTTTTCTAAGTAATGGGGAAGAGGACTGAAACATGCCACTGAAAGACTCTACTGAGACAAAAAGATGGGCTGTCAAAAAGGTAGAGGAGGTAGGATGGGCAGTTGGTCAGATCTAGTATGGATCGTACATGGACGATAGTTGGAGTCGGCGGCTCTCCT SRR6941542 testing PE reads STAR mapping to Ensembl genome Started job on | Dec 06 10:50:04 Started mapping on | Dec 06 10:50:05 Finished on | Dec 06 10:51:31 Mapping speed, Million of reads per hour | 904.89 Number of input reads | 21616806 Average input read length | 294 UNIQUE READS: Uniquely mapped reads number | 12620532 Uniquely mapped reads % | 58.38% Average mapped length | 295.44 Number of splices: Total | 2417856 Number of splices: Annotated (sjdb) | 2122791 Number of splices: GT/AG | 2244322 Number of splices: GC/AG | 28273 Number of splices: AT/AC | 11350 Number of splices: Non-canonical | 133911 Mismatch rate per base, % | 0.14% Deletion rate per base | 0.01% Deletion average length | 1.50 Insertion rate per base | 0.01% Insertion average length | 2.06 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 7646890 % of reads mapped to multiple loci | 35.37% Number of reads mapped to too many loci | 22532 % of reads mapped to too many loci | 0.10% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 5.61% % of reads unmapped: other | 0.52% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 1358958 1358958 1358958 N_multimapping 7646890 7646890 7646890 N_noFeature 5423575 12154837 5622828 N_ambiguous 509823 9232 249916 UnstrandedReadsAssigned:6687134 PositiveStrandReadsAssigned:456463 NegativeStrandReadsAssigned:6747788 Dataset is classified negative stranded MeadianReadLen=151 20thPercentileLength=151 echo kmer=147 SRR6941542 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,972 [index] number of k-mers: 66,720,672 [index] number of equivalence classes: 111,837 [quant] running in paired-end mode [quant] will process pair 1: SRR6941542-trimmed-pair1.fastq SRR6941542-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 21,616,806 reads, 12,012,913 reads pseudoaligned [quant] estimated average fragment length: 229.383 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,055 rounds 52973 SRR6941542.ke.tsv 35125 SRR6941542.se.tsv 88098 total ==> SRR6941542.ke.tsv <== target_id length eff_length est_counts tpm PNS24245 936 708.107 0 0 PNS24247 1044 815.617 3.05233 0.288764 PNS24249 1928 1699.62 7.7051 0.349803 PNS24246 1044 815.617 3.05233 0.288764 PNS24248 1044 815.617 3.05233 0.288764 PNS24244 1471 1242.62 12.1379 0.753708 PNS24243 293 102.257 0 0 KQK14069 1603 1374.62 505.088 28.3519 KQK14071 474 254.547 5.74945 1.74283 ==> SRR6941542.se.tsv <== BRADI_1g14170v3 668 BRADI_1g53295v3 10 BRADI_1g59795v3 21 BRADI_1g07683v3 0 BRADI_1g00485v3 0 BRADI_1g20270v3 46 BRADI_1g74790v3 14 BRADI_1g09890v3 0 BRADI_1g77505v3 24 BRADI_1g48960v3 0 SRR6941542 completed mapping pipeline successfully