Starting /dee2/code/volunteer_pipeline.sh SRR6941546 current disk space = 1551299571712 free memory = 1308106872 SRR6941546 SRAfilesize 38e0b6782e20b1356a684e7759f2d3d0 SRR6941546.sra SRR6941546.sra file validated SRR6941546 is paired end SRR6941546 is conventional basespace SRR6941546 read1 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR6941546_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 54 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 34.60925 35.0 35.0 35.0 35.0 35.0 2 34.52525 35.0 35.0 35.0 33.0 35.0 3 34.63875 35.0 35.0 35.0 35.0 35.0 4 34.693 35.0 35.0 35.0 35.0 35.0 5 34.678 35.0 35.0 35.0 35.0 35.0 6 39.5035 40.0 40.0 40.0 39.0 40.0 7 39.5 40.0 40.0 40.0 39.0 40.0 8 39.49175 40.0 40.0 40.0 39.0 40.0 9 39.4605 40.0 40.0 40.0 39.0 40.0 10-14 39.44235 40.0 40.0 40.0 39.0 40.0 15-19 39.366600000000005 40.0 40.0 40.0 39.0 40.0 20-24 39.39465 40.0 40.0 40.0 39.0 40.0 25-29 39.42415 40.0 40.0 40.0 39.0 40.0 30-34 39.25365 40.0 40.0 40.0 38.8 40.0 35-39 39.364050000000006 40.0 40.0 40.0 39.0 40.0 40-44 39.36775 40.0 40.0 40.0 39.0 40.0 45-49 39.34525 40.0 40.0 40.0 39.0 40.0 50-54 39.23435 40.0 40.0 40.0 39.0 40.0 55-59 39.231550000000006 40.0 40.0 40.0 39.0 40.0 60-64 39.239349999999995 40.0 40.0 40.0 39.0 40.0 65-69 39.11105 40.0 40.0 40.0 38.4 40.0 70-74 39.11875 40.0 39.8 40.0 38.4 40.0 75-79 39.05185 40.0 40.0 40.0 38.0 40.0 80-84 39.1619 40.0 40.0 40.0 38.6 40.0 85-89 39.01205 40.0 39.6 40.0 38.0 40.0 90-94 38.90315 40.0 39.2 40.0 37.2 40.0 95-99 38.92405 40.0 39.2 40.0 37.4 40.0 100-104 37.7685 39.2 38.0 39.4 34.0 39.8 105-109 38.98205 40.0 39.2 40.0 37.8 40.0 110-114 38.9626 40.0 39.6 40.0 37.6 40.0 115-119 39.0898 40.0 40.0 40.0 38.4 40.0 120-124 38.9311 40.0 39.0 40.0 37.8 40.0 125-129 38.7483 40.0 39.0 40.0 37.0 40.0 130-134 38.71040000000001 40.0 39.0 40.0 36.4 40.0 135-139 38.49185 40.0 39.0 40.0 36.0 40.0 140-144 38.1515 40.0 39.0 40.0 35.4 40.0 145-149 38.07845 40.0 39.0 40.0 35.8 40.0 150-151 35.872625 39.0 36.5 39.5 30.5 40.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 20 1.0 21 0.0 22 2.0 23 2.0 24 6.0 25 6.0 26 2.0 27 14.0 28 13.0 29 14.0 30 22.0 31 26.0 32 42.0 33 37.0 34 58.0 35 68.0 36 112.0 37 171.0 38 346.0 39 3058.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 37.73159739609414 7.886830245368053 3.355032548823235 51.02653980971458 2 19.25 8.924999999999999 33.95 37.875 3 16.575 9.375 24.75 49.3 4 23.625 19.35 18.875 38.15 5 26.200000000000003 21.5 23.849999999999998 28.449999999999996 6 26.025 23.7 25.85 24.425 7 18.775 21.2 39.375 20.65 8 20.1 17.4 32.45 30.049999999999997 9 21.025 17.95 34.125 26.900000000000002 10-14 23.52 20.669999999999998 26.41 29.4 15-19 25.09 19.765 25.405 29.74 20-24 24.538680802120318 21.823273491023652 25.05375806370956 28.58428764314647 25-29 25.352535253525353 19.34193419341934 25.992599259925992 29.312931293129314 30-34 24.52 19.165 24.67 31.645 35-39 23.455000000000002 20.29 26.51 29.744999999999997 40-44 24.36 20.97 24.535 30.135 45-49 25.619999999999997 18.485 24.92 30.975 50-54 24.435000000000002 19.35 26.545 29.67 55-59 23.189999999999998 21.095 26.169999999999998 29.544999999999998 60-64 25.72 18.47 26.534999999999997 29.275000000000002 65-69 24.349999999999998 21.4 24.62 29.630000000000003 70-74 24.315 21.135 24.805 29.744999999999997 75-79 24.805 21.66 24.115000000000002 29.42 80-84 23.119999999999997 22.715 25.019999999999996 29.145 85-89 24.92 21.665 23.375 30.04 90-94 26.26 21.385 22.945 29.409999999999997 95-99 25.629999999999995 21.26 23.799999999999997 29.310000000000002 100-104 24.795 20.64 24.95 29.615000000000002 105-109 22.875 21.725 24.615000000000002 30.785 110-114 23.07 23.189999999999998 25.515 28.225 115-119 23.385 22.195 24.709999999999997 29.709999999999997 120-124 24.139655862344938 24.719887955182074 22.18387354941977 28.956582633053223 125-129 23.118467770165523 23.008451267690152 23.293494024103616 30.579586938040705 130-134 24.63 21.029999999999998 22.75 31.59 135-139 23.715 22.34 24.44 29.505 140-144 25.195 22.785 23.175 28.845 145-149 22.189999999999998 23.66 25.355 28.794999999999998 150-151 22.075 23.275000000000002 24.55 30.099999999999998 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.5 8 0.5 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.5 23 1.0 24 0.5 25 0.0 26 1.0 27 1.0 28 0.0 29 0.0 30 1.0 31 3.0 32 5.5 33 5.5 34 5.5 35 6.5 36 12.0 37 19.0 38 22.5 39 22.0 40 19.0 41 20.0 42 28.5 43 35.5 44 34.5 45 52.5 46 80.5 47 83.5 48 68.5 49 86.0 50 113.5 51 139.5 52 155.5 53 198.0 54 265.0 55 415.0 56 483.5 57 348.5 58 281.5 59 276.0 60 197.0 61 111.5 62 76.0 63 67.5 64 52.5 65 30.0 66 23.5 67 15.5 68 15.5 69 9.0 70 10.5 71 16.0 72 26.0 73 24.5 74 9.5 75 8.5 76 9.0 77 3.5 78 1.0 79 0.5 80 0.5 81 0.5 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.15 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.015 25-29 0.01 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.04 125-129 0.015 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.225 #Duplication Level Percentage of deduplicated Percentage of total 1 72.47534053546266 38.574999999999996 2 12.30624706434946 13.100000000000001 3 5.542508219821513 8.85 4 2.8182245185533117 6.0 5 2.0666979802724286 5.5 6 1.2212306247064348 3.9 7 0.7984969469234382 2.9749999999999996 8 0.7045561296383279 3.0 9 0.23485204321277595 1.125 >10 1.737905119774542 13.775 >50 0.09394081728511039 3.2 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GGCATGATGACTTGGCCTCATCCTCTCCTTCCTCCGGCTTAACACCGGCG 70 1.7500000000000002 No Hit CCCCACTGCTGCCTCCCGTAGGAGTCTGGGCCGTGTCTCAGTCCCAGTGT 58 1.4500000000000002 No Hit CCCACTGCTGCCTCCCGTAGGAGTCTGGGCCGTGTCTCAGTCCCAGTGTG 43 1.075 No Hit CCCGTCAATTCCTTTGAGTTTCATTCTTGCGAACGTACTCCCCAGGCGGG 31 0.775 No Hit CCTCAGCCTACGGGGTATTAGCAACCGTTTCCAGTTGTTGTTCCCCTCCC 24 0.6 No Hit CTCCTTTTGCTCCTCAGCCTACGGGGTATTAGCAACCGTTTCCAGTTGTT 21 0.525 No Hit CCGGGAACGGATTCACCGCCGTATGGCTGACCGGCGATTACTAGCGATTC 21 0.525 No Hit GTCCAGTCAACTGCTGCGCCTCAACGCATTTCGGGGAGAACCAGCTAGCT 19 0.475 No Hit CCATCGTTTACGGCTAGGACTACTGGGGTCTCTAATCCCATTTGCTCCCC 18 0.44999999999999996 No Hit GTTCGAGCTTTTCCTGGGAGTATGGCATCGGTTACATACTTCAGTGCCGT 18 0.44999999999999996 No Hit CTCCACGCGGCATTGCTCCGTCAGGCTTTCGCCCATTGCGGAAAATTCCC 18 0.44999999999999996 No Hit GGGGCATGATGACTTGGCCTCATCCTCTCCTTCCTCCGGCTTAACACCGG 16 0.4 No Hit GTCCCAGTGTGGCTGATCATCCTCTCGGACCAGCTACTGATCATCGCCTT 16 0.4 No Hit CCGTCAATTCCTTTGAGTTTCATTCTTGCGAACGTACTCCCCAGGCGGGA 15 0.375 No Hit GTTCGTTAACGGAATTAACCAGACAAATCGCTCCACCAACTAAGAACGGC 15 0.375 No Hit CTTTCTTTTCCTCTGGCTACTAAGATGTTTCAGTTCGCCAGGTTGTCTCT 15 0.375 No Hit CCTAGATGTCCAGTCAACTGCTGCGCCTCAACGCATTTCGGGGAGAACCA 14 0.35000000000000003 No Hit GTCGGTTTCGGGTACAGGTACCCTTTTGTTGAAGGTCGTTCGAGCTTTTC 14 0.35000000000000003 No Hit GTCCCTATTAATCATTACTCCGATCCCGAAGGCCAACACAATAGGACCGG 14 0.35000000000000003 No Hit CCCATCACGATGAAATTTCCCAAGATTACCCGGGCCTGTCGGCCAAGGCT 14 0.35000000000000003 No Hit CTCCTCAGCCTACGGGGTATTAGCAACCGTTTCCAGTTGTTGTTCCCCTC 13 0.325 No Hit GCTCCTCAGCCTACGGGGTATTAGCAACCGTTTCCAGTTGTTGTTCCCCT 13 0.325 No Hit GCTTTCTTTTCCTCTGGCTACTAAGATGTTTCAGTTCGCCAGGTTGTCTC 12 0.3 No Hit GGTCGTTCGAGCTTTTCCTGGGAGTATGGCATCGGTTACATACTTCAGTG 12 0.3 No Hit CTCCTTTATCACTGAGCGGTCATTTAGGGGCCTTAGCTGGTGATCCGGGC 11 0.27499999999999997 No Hit GTCGGGGCAGGCGGCGGGCGCAGGCGCCGCTTGCTAGCTTGGATTCTGAC 11 0.27499999999999997 No Hit GTTCCCTTAACCAAGCCACTGCCTATGAGTCGCCGGCTCATTCTTCAACA 11 0.27499999999999997 No Hit CTCAGATACCGTCATTGTTTCTTCTCCGAGAAAAGAAGTTGACGACCCGT 11 0.27499999999999997 No Hit AGCACGTGTGTCGCCCAGGGCATAAGGGGCATGATGACTTGGCCTCATCC 11 0.27499999999999997 No Hit CTCCTACTCATCGGGGCATGGCGCTCGCCCAGATGGCCGGGTGTGGGTCG 10 0.25 No Hit CTCCCCTAGCTTTCGTCTCTCAGTGTCAGTGTCGGCCCAGCAGAGTGCTT 10 0.25 No Hit CCTCGCGGTACTTGTTCGCTATCGGTCTCTCGCCTGTATTTAGCCTTGGA 10 0.25 No Hit GTCCATCGACTACGCCTTTCGGCCTGATCTTAGGCCCTGACTCACCCTCC 10 0.25 No Hit GTTCAGGGTTCCAAACTCATAGTGGCAACTAAACACGAGGGTTGCGCTCG 10 0.25 No Hit GTGTCCTTAAACCTATAACCATCTTTCGGCTAACCTAGCCTCCTCCGTCC 10 0.25 No Hit GGCAACTAAACACGAGGGTTGCGCTCGTTGCGAGACTTAACCCAACACCT 10 0.25 No Hit CCCTAGAGTAACTTTTATCCGTTGAGCGACGGCCCTTCCACTCGGCACCG 10 0.25 No Hit CTCTGCCCCTACCGTACTCCAGCTTGGTAGTTTCCACCGCCTGTCCAGGG 10 0.25 No Hit GGGGAAGGGAGCTTCGAGGCGGCCGGACGCGGCTCGTCGGCCGGAACGGC 10 0.25 No Hit ATCGACTACGCCTTTCGGCCTGATCTTAGGCCCTGACTCACCCTCCGTGG 9 0.22499999999999998 No Hit CCTAGAGTAACTTTTATCCGTTGAGCGACGGCCCTTCCACTCGGCACCGT 9 0.22499999999999998 No Hit GTGGCAACTAAACACGAGGGTTGCGCTCGTTGCGAGACTTAACCCAACAC 9 0.22499999999999998 No Hit CCCGGAACCCAAAGACTTTGATTTCTCATAAGGTGCCGGCGGAGTCCTAT 9 0.22499999999999998 No Hit GTCCTCTCAATGCTCTAACGCCCACACCGGATATGGACCGAACTGTCTCA 9 0.22499999999999998 No Hit CTCCAGACTACAATTCGGACGGCACGGCCGCCCGATTCTCAAGCTGGGCT 8 0.2 No Hit CCCGAAGTTACGGATCCGTTTTGCCGACTTCCCTTGCCTACATTGTTCCA 8 0.2 No Hit GTCATTGTTTCTTCTCCGAGAAAAGAAGTTGACGACCCGTAGGCCTTCCA 8 0.2 No Hit CCCCGGAACCCAAAGACTTTGATTTCTCATAAGGTGCCGGCGGAGTCCTA 8 0.2 No Hit GTACAAAGGGCAGGGACGTAGTCAACGCGAGCTGATGACTCGCGCTTACT 8 0.2 No Hit CTCACGTACCGCATTAATGGGCGAACAGCCCAACCCTTGGAACCACCTAC 8 0.2 No Hit GCTCCGTCAGGCTTTCGCCCATTGCGGAAAATTCCCCACTGCTGCCTCCC 8 0.2 No Hit CCTCACGGTACTACTTCGCTATCGGTCACCCAGGAGTATTTAGCCTTGCA 8 0.2 No Hit CCCCCGTCAATTCCTTTGAGTTTCATTCTTGCGAACGTACTCCCCAGGCG 8 0.2 No Hit CCCCGTTCATCTTCAGCGCAAGGGCGCTCGATCAGTGAGCTATTACGCAC 8 0.2 No Hit CCTCCACGCGGCATTGCTCCGTCAGGCTTTCGCCCATTGCGGAAAATTCC 8 0.2 No Hit CTAGCTTTCGTCTCTCAGTGTCAGTGTCGGCCCAGCAGAGTGCTTTCGCC 8 0.2 No Hit CCCCTACCGTACTCCAGCTTGGTAGTTTCCACCGCCTGTCCAGGGTTGAG 8 0.2 No Hit CCTAGCTTTCGTCTCTCAGTGTCAGTGTCGGCCCAGCAGAGTGCTTTCGC 8 0.2 No Hit CCTGTGTCGGTTTCGGGTACAGGTACCCTTTTGTTGAAGGTCGTTCGAGC 8 0.2 No Hit CCCAATCATTCCGGATAACGCTTGCATCCTCTGTCTTACCGCGGCTGCTG 7 0.17500000000000002 No Hit GTGTCGCCCAGGGCATAAGGGGCATGATGACTTGGCCTCATCCTCTCCTT 7 0.17500000000000002 No Hit CTCCACCGCTTGTGCGGGCCCCCGTCAATTCCTTTGAGTTTCATTCTTGC 7 0.17500000000000002 No Hit GTTCTATTTCACTACCCACTGGGGGTTCTTTTCACCTTTCCCTCACGGTA 7 0.17500000000000002 No Hit CGTTGAGCGACGGCCCTTCCACTCGGCACCGTCGGATCACTAAGGCCGAC 7 0.17500000000000002 No Hit GGGAAGGGAGCTTCGAGGCGGCCGGACGCGGCTCGTCGGCCGGAACGGCT 7 0.17500000000000002 No Hit CCCGGGAACGGATTCACCGCCGTATGGCTGACCGGCGATTACTAGCGATT 7 0.17500000000000002 No Hit CTCCCATTTCGCTCGCCGCTACTACGGGAATCGCTTTTGCTTTCTTTTCC 7 0.17500000000000002 No Hit GCCACCTACAGACGCTTTACGCCCAATCATTCCGGATAACGCTTGCATCC 7 0.17500000000000002 No Hit CCTATTTGGGAATCTCCGGATCTATGCTTATTTTCAACTCCCCGAAGCAT 7 0.17500000000000002 No Hit CCTGCTTCATGCAGGCGAGTTGCAGCCTGCAATCCGAACTGAGGACGGGT 7 0.17500000000000002 No Hit CCTGGGAGTATGGCATCGGTTACATACTTCAGTGCCGTAGCGCCTGGTAT 7 0.17500000000000002 No Hit CATCGTTTACGGCTAGGACTACTGGGGTCTCTAATCCCATTTGCTCCCCT 7 0.17500000000000002 No Hit CTCAGTGTCAGTGTCGGCCCAGCAGAGTGCTTTCGCCGTTGGTGTTCTTT 7 0.17500000000000002 No Hit CTGTTGTCCATCGACTACGCCTTTCGGCCTGATCTTAGGCCCTGACTCAC 7 0.17500000000000002 No Hit CCCCTACCGATGCATTTTGACATCCCACAGCTTCGGCAGATCGCTTAGCC 7 0.17500000000000002 No Hit GCTCATTCTTCAACAGGCACGCGGTCAGAGATCACTTTCCCCTCCCACTG 7 0.17500000000000002 No Hit CCCGATTCCATGGCGCGGCTCACCGGAGCAGCCGCGCCGTCCTACCTATT 6 0.15 No Hit CACCGCTTGTGCGGGCCCCCGTCAATTCCTTTGAGTTTCATTCTTGCGAA 6 0.15 No Hit GCATCGTTTATGGTTGAGACTAGGACGGTATCTGATCGTCTTCGAGCCCC 6 0.15 No Hit CGTGGGTCGGGGCAGGCGGCGGGCGCAGGCGCCGCTTGCTAGCTTGGATT 6 0.15 No Hit ATCCTCTCCGCACTTGGCTACCCAGCGTTTACCGTAGGCACGATAACTGG 6 0.15 No Hit GTCCCTCTAAGAAGCTAGCTGCGGAGGGATGGCTCCGCATAGCTAGTTAG 6 0.15 No Hit CACCGCTCCACCGGAAATTCCCTCTGCCCCTACCGTACTCCAGCTTGGTA 6 0.15 No Hit CCCCAACTTTCGTTCTTGATTAATGAAAACATCCTTGGCAAATGCTTTCG 6 0.15 No Hit GTGTCGGTTTCGGGTACAGGTACCCTTTTGTTGAAGGTCGTTCGAGCTTT 6 0.15 No Hit GACCTATTTGGGAATCTCCGGATCTATGCTTATTTTCAACTCCCCGAAGC 6 0.15 No Hit GGGCATGATGACTTGGCCTCATCCTCTCCTTCCTCCGGCTTAACACCGGC 6 0.15 No Hit CCTCCGTCCCTCCGTACCAACAAGGGGTAGTACAGGAATATTGACCTGTT 6 0.15 No Hit CGTCTCTCAGTGTCAGTGTCGGCCCAGCAGAGTGCTTTCGCCGTTGGTGT 6 0.15 No Hit CGTCGAGTTATCATGAATCATCGGATCAGCGAGCAAAGCCCGCGTCAGCC 6 0.15 No Hit CGTTAACGGAATTAACCAGACAAATCGCTCCACCAACTAAGAACGGCCAT 6 0.15 No Hit GTACAAGGCCCGGGAACGGATTCACCGCCGTATGGCTGACCGGCGATTAC 6 0.15 No Hit CCTCCTTGGGCGGATTTCTCCTTTTGCTCCTCAGCCTACGGGGTATTAGC 6 0.15 No Hit CAGGGTTCCAAACTCATAGTGGCAACTAAACACGAGGGTTGCGCTCGTTG 6 0.15 No Hit GGTGTGTACAAGGCCCGGGAACGGATTCACCGCCGTATGGCTGACCGGCG 6 0.15 No Hit GTTCCAAACTCATAGTGGCAACTAAACACGAGGGTTGCGCTCGTTGCGAG 6 0.15 No Hit GTCGTCTGCAAAGGATTCAGCCCGCCGCCCGTGGGGAAGGGAGCTTCGAG 6 0.15 No Hit CCCTATTAATCATTACTCCGATCCCGAAGGCCAACACAATAGGACCGGAA 6 0.15 No Hit CCAACACCTTACGGCACGAGCTGACGACAGCCATGCACCACCTGTGTCCG 6 0.15 No Hit ATGATGACTTGGCCTCATCCTCTCCTTCCTCCGGCTTAACACCGGCGGTC 6 0.15 No Hit GTCCTTAAACCTATAACCATCTTTCGGCTAACCTAGCCTCCTCCGTCCCT 6 0.15 No Hit CCGGGCTGTTTCCCTCTCGACGATGAAGCTTATCCCCCATCGTCTCACTG 6 0.15 No Hit CTCTGTCTTACCGCGGCTGCTGGCACAGAGTTAGCCGATGCTTATTCCTC 5 0.125 No Hit CTTTTGTTCCACACGAGATTTCTGTTCTCGTTGAGCTCATCTTAGGACAC 5 0.125 No Hit CCACTGCTGCCTCCCGTAGGAGTCTGGGCCGTGTCTCAGTCCCAGTGTGG 5 0.125 No Hit GCCCAGGCAGGCGTGCCCTCGACCGGGTGGCCTCGGGCGCAACTTGCGTT 5 0.125 No Hit GCCCCGTTCATCTTCAGCGCAAGGGCGCTCGATCAGTGAGCTATTACGCA 5 0.125 No Hit GTGTGTACAAAGGGCAGGGACGTAGTCAACGCGAGCTGATGACTCGCGCT 5 0.125 No Hit GGAGGGGCTGCGATGAAGGCGATAATAAATACAGAAGTTGCGGTCAATAA 5 0.125 No Hit CCCGCTTCCGACCCACGGAATAAGTAAAATAACGTTAAAAGTAGTGGTAT 5 0.125 No Hit CCCGGCTTCCGGTTCATCCCGCATCGCCAGTTCTGCTTACCAAAAATGGC 5 0.125 No Hit GGCATCACAGACCTGTTATTGCCTCAAACTTCCGTCGCCTAAACGGCGAT 5 0.125 No Hit CCCAGGTTCGGGTCCATAAGCAGTGACAATCGCCCTATGAAGACTCGCTT 5 0.125 No Hit CTCTCCCACAACCCCGTTTTCACGGTTTAGGCTGCTCCCATTTCGCTCGC 5 0.125 No Hit ACATGCTCCACCGCTTGTGCGGGCCCCCGTCAATTCCTTTGAGTTTCATT 5 0.125 No Hit GTCTCTCAGTGTCAGTGTCGGCCCAGCAGAGTGCTTTCGCCGTTGGTGTT 5 0.125 No Hit CTGGGAGTATGGCATCGGTTACATACTTCAGTGCCGTAGCGCCTGGTATG 5 0.125 No Hit CCTCAACGCATTTCGGGGAGAACCAGCTAGCTCTGGGTTCGAGTGGCATT 5 0.125 No Hit CGTGTGTCGCCCAGGGCATAAGGGGCATGATGACTTGGCCTCATCCTCTC 5 0.125 No Hit GCTGTGGTTTCGCTGGATAGTAGACAGGGACAGTGGGAATCTCGTTAATC 5 0.125 No Hit GACCTGTTGTCCATCGACTACGCCTTTCGGCCTGATCTTAGGCCCTGACT 5 0.125 No Hit CGGCTAACCTAGCCTCCTCCGTCCCTCCGTACCAACAAGGGGTAGTACAG 5 0.125 No Hit CCTAATTCTCCGTCACCCGTCACCACCATGGTAGGCCCCTATCCTACCAT 5 0.125 No Hit CTTAAACCTATAACCATCTTTCGGCTAACCTAGCCTCCTCCGTCCCTCCG 5 0.125 No Hit CCCCGCTGCTGCCTCCCGTAGGAGTCTGGGCCGTGTCTCAGTCCCAGTGT 5 0.125 No Hit CACCTCCACGCGGCATTGCTCCGTCAGGCTTTCGCCCATTGCGGAAAATT 5 0.125 No Hit CCTCAGATACCGTCATTGTTTCTTCTCCGAGAAAAGAAGTTGACGACCCG 5 0.125 No Hit CCGGCGATTACTAGCGATTCCTGCTTCATGCAGGCGAGTTGCAGCCTGCA 5 0.125 No Hit GCCCAATCATTCCGGATAACGCTTGCATCCTCTGTCTTACCGCGGCTGCT 5 0.125 No Hit GCCCTATGAAGACTCGCTTTCGCTACGGCTCCGGTGGGTTCCGTTCCCTT 5 0.125 No Hit CCGTCATTGTTTCTTCTCCGAGAAAAGAAGTTGACGACCCGTAGGCCTTC 5 0.125 No Hit CTTCGCTATCGGTCACCCAGGAGTATTTAGCCTTGCAAGGTGGTCCTTGC 5 0.125 No Hit CGCTATCGGTCACCCAGGAGTATTTAGCCTTGCAAGGTGGTCCTTGCTGA 5 0.125 No Hit CGCCCAATCATTCCGGATAACGCTTGCATCCTCTGTCTTACCGCGGCTGC 5 0.125 No Hit CCCTAGCTTTCGTCTCTCAGTGTCAGTGTCGGCCCAGCAGAGTGCTTTCG 5 0.125 No Hit GTCCCGCCCATTGTAGCACGTGTGTCGCCCAGGGCATAAGGGGCATGATG 5 0.125 No Hit CTCCGTACCAACAAGGGGTAGTACAGGAATATTGACCTGTTGTCCATCGA 5 0.125 No Hit CCCCGTCAATTCCTTTGAGTTTCATTCTTGCGAACGTACTCCCCAGGCGG 5 0.125 No Hit CACGGGATTCCACGTGCCCCATGCTACTCGGGTCAGAGCGTAAGCTAGTG 5 0.125 No Hit GTCCCGACAGGCGTGCTCCAACTCGAACCCTTCACAGAAGATCAGGGTCG 5 0.125 No Hit CTCGTTGAATACATCAGTGTAGCGCGCGTGCGGCCCAGAACATCTAAGGG 5 0.125 No Hit ATTAATCATTACTCCGATCCCGAAGGCCAACACAATAGGACCGGAATCCT 5 0.125 No Hit CACTGCTGCCTCCCGTAGGAGTCTGGGCCGTGTCTCAGTCCCAGTGTGGC 5 0.125 No Hit CCCTAGATGTCCAGTCAACTGCTGCGCCTCAACGCATTTCGGGGAGAACC 5 0.125 No Hit CTCAGCCTACGGGGTATTAGCAACCGTTTCCAGTTGTTGTTCCCCTCCCA 5 0.125 No Hit GGGTCTTTCTGTCCAGGTGCAGGTAGTCCGCATCTTCACAGACATGTCTA 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.025 0.0 0.0 0.0 0.0 2 0.025 0.0 0.0 0.0 0.0 3 0.025 0.0 0.0 0.0 0.0 4 0.025 0.0 0.0 0.0 0.0 5 0.025 0.0 0.0 0.0 0.0 6 0.025 0.0 0.0 0.0 0.0 7 0.025 0.0 0.0 0.0 0.0 8 0.025 0.0 0.0 0.0 0.0 9 0.025 0.0 0.0 0.0 0.0 10-11 0.025 0.0 0.0 0.0 0.0 12-13 0.025 0.0 0.0 0.0 0.0 14-15 0.025 0.0 0.0 0.0 0.0 16-17 0.025 0.0 0.0 0.0 0.0 18-19 0.025 0.0 0.0 0.0 0.0 20-21 0.025 0.0 0.0 0.0 0.0 22-23 0.025 0.0 0.0 0.0 0.0 24-25 0.025 0.0 0.0 0.0 0.0 26-27 0.025 0.0 0.0 0.0 0.0 28-29 0.05 0.0 0.0 0.0 0.0 30-31 0.075 0.0 0.0 0.0 0.0 32-33 0.075 0.0 0.0 0.0 0.0 34-35 0.075 0.0 0.0 0.0 0.0 36-37 0.075 0.0 0.0 0.0 0.0 38-39 0.075 0.0 0.0 0.0 0.0 40-41 0.0875 0.0 0.0 0.0 0.0 42-43 0.1125 0.0 0.0 0.0 0.0 44-45 0.125 0.0 0.0 0.0 0.0 46-47 0.1375 0.0 0.0 0.0 0.0 48-49 0.175 0.0 0.0 0.0 0.0 50-51 0.2 0.0 0.0 0.0 0.0 52-53 0.2 0.0 0.0 0.0 0.0 54-55 0.2375 0.0 0.0 0.0 0.0 56-57 0.3 0.0 0.0 0.0 0.0 58-59 0.35 0.0 0.0 0.0 0.0 60-61 0.4 0.0 0.0 0.0 0.0 62-63 0.48750000000000004 0.0 0.0 0.0 0.0 64-65 0.675 0.0 0.0 0.0 0.0 66-67 0.8125 0.0 0.0 0.0 0.0 68-69 0.8625 0.0 0.0 0.0 0.0 70-71 1.025 0.0 0.0 0.0 0.0 72-73 1.2 0.0 0.0 0.0 0.0 74-75 1.4249999999999998 0.0 0.0 0.0 0.0 76-77 1.675 0.0 0.0 0.0 0.0 78-79 1.9500000000000002 0.0 0.0 0.0 0.0 80-81 2.225 0.0 0.0 0.0 0.0 82-83 2.6125 0.0 0.0 0.0 0.0 84-85 2.9875 0.0 0.0 0.0 0.0 86-87 3.45 0.0 0.0 0.0 0.0 88-89 4.15 0.0 0.0 0.0 0.0 90-91 4.975 0.0 0.0 0.0 0.0 92-93 5.3875 0.0 0.0 0.0 0.0 94-95 6.075 0.0 0.0 0.0 0.0 96-97 6.7375 0.0 0.0 0.0 0.0 98-99 7.487500000000001 0.0 0.0 0.0 0.0 100-101 8.275 0.0 0.0 0.0 0.0 102-103 9.1125 0.0 0.0 0.0 0.0 104-105 10.075 0.0 0.0 0.0 0.0 106-107 10.962499999999999 0.0 0.0 0.0 0.0 108-109 11.7375 0.0 0.0 0.0 0.0 110-111 12.925 0.0 0.0 0.0 0.0 112-113 14.15 0.0 0.0 0.0 0.0 114-115 15.2375 0.0 0.0 0.0 0.0 116-117 16.075 0.0 0.0 0.0 0.0 118-119 17.05 0.0 0.0 0.0 0.0 120-121 17.8125 0.0 0.0 0.0 0.0 122-123 18.65 0.0 0.0 0.0 0.0 124-125 19.675 0.0 0.0 0.0 0.0 126-127 20.85 0.0 0.0 0.0 0.0 128-129 21.8125 0.0 0.0 0.0 0.0 130-131 22.9125 0.0 0.0 0.0 0.0 132-133 23.799999999999997 0.0 0.0 0.0 0.0 134-135 24.9125 0.0 0.0 0.0 0.0 136-137 26.225 0.0 0.0 0.0 0.0 138-139 27.2375 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCCTCGA 10 0.006830828 145.0 145 GAACGCT 10 0.006830828 145.0 9 GTCACAT 75 0.0012377208 13.533334 140-144 CTCCAGT 85 0.0031733946 11.941176 135-139 >>END_MODULE SRR6941546 read2 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR6941546_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 54 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 34.02125 35.0 35.0 35.0 32.0 35.0 2 34.37125 35.0 35.0 35.0 33.0 35.0 3 34.49325 35.0 35.0 35.0 34.0 35.0 4 34.34 35.0 35.0 35.0 33.0 35.0 5 34.3995 35.0 35.0 35.0 33.0 35.0 6 39.07025 40.0 40.0 40.0 38.0 40.0 7 38.99125 40.0 39.0 40.0 38.0 40.0 8 39.0625 40.0 40.0 40.0 39.0 40.0 9 39.20025 40.0 40.0 40.0 39.0 40.0 10-14 39.2509 40.0 40.0 40.0 39.0 40.0 15-19 39.2798 40.0 40.0 40.0 39.0 40.0 20-24 39.3082 40.0 40.0 40.0 39.0 40.0 25-29 39.21745 40.0 40.0 40.0 38.8 40.0 30-34 39.212250000000004 40.0 40.0 40.0 39.0 40.0 35-39 39.0465 40.0 39.6 40.0 38.0 40.0 40-44 39.09565 40.0 39.6 40.0 38.6 40.0 45-49 39.114850000000004 40.0 39.6 40.0 38.2 40.0 50-54 38.879 40.0 39.0 40.0 37.8 40.0 55-59 38.782149999999994 40.0 39.0 40.0 37.0 40.0 60-64 38.828649999999996 40.0 39.0 40.0 37.2 40.0 65-69 38.85295 40.0 39.2 40.0 37.2 40.0 70-74 38.57505 40.0 39.0 40.0 36.2 40.0 75-79 38.56125 40.0 39.0 40.0 36.2 40.0 80-84 38.68975 40.0 39.0 40.0 36.8 40.0 85-89 38.814499999999995 40.0 39.0 40.0 36.8 40.0 90-94 38.76235 40.0 39.0 40.0 36.8 40.0 95-99 38.36625 40.0 39.0 40.0 35.8 40.0 100-104 36.94615 38.6 37.4 39.2 33.0 39.4 105-109 38.29135 40.0 39.0 40.0 35.6 40.0 110-114 38.3425 40.0 39.0 40.0 35.6 40.0 115-119 38.0647 40.0 39.0 40.0 35.0 40.0 120-124 37.8147 40.0 39.0 40.0 34.4 40.0 125-129 37.670249999999996 40.0 39.0 40.0 34.2 40.0 130-134 37.104499999999994 40.0 38.8 40.0 32.8 40.0 135-139 36.4949 40.0 38.2 40.0 30.4 40.0 140-144 35.50320000000001 39.0 37.4 40.0 23.0 40.0 145-149 34.298950000000005 39.0 36.2 40.0 9.6 40.0 150-151 29.86975 36.5 19.0 39.5 2.0 40.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 1.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 1.0 12 0.0 13 0.0 14 0.0 15 0.0 16 1.0 17 0.0 18 1.0 19 2.0 20 2.0 21 6.0 22 9.0 23 12.0 24 12.0 25 9.0 26 13.0 27 21.0 28 26.0 29 35.0 30 34.0 31 41.0 32 51.0 33 80.0 34 100.0 35 115.0 36 160.0 37 300.0 38 486.0 39 2482.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.07591754650578 17.92357968828557 5.027652086475616 35.97285067873303 2 31.505133984472828 23.741547708489858 25.66992236413724 19.083395942900076 3 24.425 24.5 26.025 25.05 4 30.3 28.875 20.225 20.599999999999998 5 31.924999999999997 28.549999999999997 18.025 21.5 6 27.3 35.075 14.7 22.925 7 23.724999999999998 22.725 29.075 24.474999999999998 8 25.85 22.45 23.3 28.4 9 31.25 20.7 23.425 24.625 10-14 30.335 24.79 20.24 24.635 15-19 30.4 25.155 20.64 23.805 20-24 29.5 25.509999999999998 20.615 24.375 25-29 29.794999999999998 25.319999999999997 20.605 24.279999999999998 30-34 29.520000000000003 25.724999999999998 20.565 24.19 35-39 30.159999999999997 27.034999999999997 19.88 22.925 40-44 31.014999999999997 27.195000000000004 19.41 22.38 45-49 30.385 26.47 19.580000000000002 23.565 50-54 30.535 25.05 20.305 24.11 55-59 29.970000000000002 26.095000000000002 20.65 23.285 60-64 31.435000000000002 24.529999999999998 20.65 23.385 65-69 32.105 25.380000000000003 19.615 22.900000000000002 70-74 31.895 24.67 20.435 23.0 75-79 30.570000000000004 24.52 20.865000000000002 24.044999999999998 80-84 30.955 24.93 20.75 23.365 85-89 30.59 24.68 20.974999999999998 23.755000000000003 90-94 30.91 25.380000000000003 19.965 23.745 95-99 31.724999999999998 24.865000000000002 20.369999999999997 23.04 100-104 31.31 27.6 19.155 21.935 105-109 31.014999999999997 24.765 21.435000000000002 22.785 110-114 32.095 25.374999999999996 20.845 21.685 115-119 32.095 25.805 20.3 21.8 120-124 31.540000000000003 25.745 19.655 23.06 125-129 31.755 26.33 18.98 22.935 130-134 32.17 25.435000000000002 19.33 23.064999999999998 135-139 31.874999999999996 25.259999999999998 19.485 23.380000000000003 140-144 33.48 24.875 21.060000000000002 20.585 145-149 32.925 25.324999999999996 20.685000000000002 21.065 150-151 33.225 26.650000000000002 17.849999999999998 22.275 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1.0 1 0.5 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.5 22 0.5 23 0.0 24 0.5 25 1.5 26 1.0 27 0.5 28 1.0 29 1.0 30 1.5 31 2.5 32 3.5 33 5.5 34 6.0 35 10.0 36 16.0 37 14.5 38 16.5 39 33.0 40 34.0 41 22.0 42 24.5 43 34.0 44 42.0 45 50.0 46 57.5 47 78.5 48 88.5 49 105.5 50 139.5 51 161.5 52 170.5 53 240.5 54 348.5 55 428.5 56 408.0 57 291.5 58 236.0 59 212.0 60 171.0 61 127.5 62 108.0 63 70.0 64 27.5 65 18.0 66 15.0 67 25.0 68 31.5 69 23.5 70 18.0 71 13.0 72 7.5 73 11.5 74 10.5 75 8.0 76 8.5 77 6.0 78 6.0 79 3.5 80 0.0 81 0.0 82 0.0 83 0.5 84 0.5 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.5499999999999999 2 0.17500000000000002 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.25000000000001 #Duplication Level Percentage of deduplicated Percentage of total 1 73.0204081632653 44.725 2 15.591836734693878 19.1 3 5.061224489795919 9.3 4 2.571428571428571 6.3 5 1.5102040816326532 4.625 6 0.36734693877551017 1.35 7 0.5714285714285714 2.45 8 0.2857142857142857 1.4000000000000001 9 0.20408163265306123 1.125 >10 0.7346938775510203 6.9750000000000005 >50 0.0816326530612245 2.65 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTTCGATCCTGGCTCAGGATGAACGCTGGCGGCATGCTTAACACATGCAA 55 1.375 No Hit CCTTACCAGGGCTTGACATGCCGCGAATCCTCTTGAAAGAGAGGGGTGCC 51 1.275 No Hit GGCTTGCGGTGGATACCTAGGCACCCAGAGACGAGGAAGGGCGTAGCAAG 34 0.8500000000000001 No Hit CAGTGACCAGGCCCGGGCGACTGTTTACCAAAAACACAGGTCTCCGCAAA 24 0.6 No Hit GAACCTTACCAGGGCTTGACATGCCGCGAATCCTCTTGAAAGAGAGGGGT 21 0.525 No Hit AGAACCTTACCAGGGCTTGACATGCCGCGAATCCTCTTGAAAGAGAGGGG 19 0.475 No Hit CTTACCAGGTCCAGACATAGCAAGGATTGACAGACTGAGAGCTCTTTCTT 18 0.44999999999999996 No Hit CTTACCAGGGCTTGACATGCCGCGAATCCTCTTGAAAGAGAGGGGTGCCC 16 0.4 No Hit GGATGAACGCTGGCGGCATGCTTAACACATGCAAGTCGAACGGGAAGTGG 15 0.375 No Hit CCTGCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGCAA 15 0.375 No Hit CGGGTGAGTAACGCGTAAGAACCTGCCCTTGGGAGGGGAACAACAACTGG 15 0.375 No Hit ACCTTACCAGGGCTTGACATGCCGCGAATCCTCTTGAAAGAGAGGGGTGC 14 0.35000000000000003 No Hit CAGGGCTTGACATGCCGCGAATCCTCTTGAAAGAGAGGGGTGCCCTCGGG 12 0.3 No Hit CAACAACTGGAAACGGTTGCTAATACCCCGTAGGCTGAGGAGCAAAAGGA 12 0.3 No Hit CCTGGCTCAGGATGAACGCTGGCGGCATGCTTAACACATGCAAGTCGAAC 11 0.27499999999999997 No Hit CTTGCGGTGGATACCTAGGCACCCAGAGACGAGGAAGGGCGTAGCAAGCG 11 0.27499999999999997 No Hit GGCGGGACTACCCGCTGAGTTTAAGCATATAAATAAGCGGAGGAGAAGAA 11 0.27499999999999997 No Hit CGATCCTGGCTCAGGATGAACGCTGGCGGCATGCTTAACACATGCAAGTC 11 0.27499999999999997 No Hit GGGAATTTCCGGTGGAGCGGTGAAATGCATTGAGATCGGAAAGAACACCA 10 0.25 No Hit GATGAACGCTGGCGGCATGCTTAACACATGCAAGTCGAACGGGAAGTGGT 10 0.25 No Hit GGAACAACAACTGGAAACGGTTGCTAATACCCCGTAGGCTGAGGAGCAAA 9 0.22499999999999998 No Hit TGAACGCTGGCGGCATGCTTAACACATGCAAGTCGAACGGGAAGTGGTGT 9 0.22499999999999998 No Hit AAGAACCTTACCAGGGCTTGACATGCCGCGAATCCTCTTGAAAGAGAGGG 9 0.22499999999999998 No Hit GATCCTGGCTCAGGATGAACGCTGGCGGCATGCTTAACACATGCAAGTCG 9 0.22499999999999998 No Hit CGAAAACATTGGTGAGAATCCAATGCCCCGAAAACCCAAGGTTTCCTCCG 9 0.22499999999999998 No Hit ACCAGGTCCAGACATAGCAAGGATTGACAGACTGAGAGCTCTTTCTTGAT 8 0.2 No Hit CATGCCGCGAATCCTCTTGAAAGAGAGGGGTGCCCTCGGGAACGCGGACA 8 0.2 No Hit GTTTAAGGACACAAGGTGACCCTGCTTTTTCAGGGTAAGAAGGGGTAGAG 8 0.2 No Hit GGAAGGCCTACGGGTCGTCAACTTCTTTTCTCGGAGAAGAAACAATGACG 8 0.2 No Hit CTCGCAACGAGCGCAACCCTCGTGTTTAGTTGCCACTATGAGTTTGGAAC 8 0.2 No Hit AAGGCTTGCGGTGGATACCTAGGCACCCAGAGACGAGGAAGGGCGTAGCA 8 0.2 No Hit AGAGTTCGATCCTGGCTCAGGATGAACGCTGGCGGCATGCTTAACACATG 8 0.2 No Hit CGGATATCTCGGCTCTCGCATCGATGAAGAACGTAGCGAAATGCGATACC 7 0.17500000000000002 No Hit CTCAGGATGAACGCTGGCGGCATGCTTAACACATGCAAGTCGAACGGGAA 7 0.17500000000000002 No Hit GCCTGACGGAGCAATGCCGCGTGGAGGTGGAAGGCCTACGGGTCGTCAAC 7 0.17500000000000002 No Hit GGGAAGCAACCGCGAAAGCGGGGGTCGACGAAGCGGAAGCGAGAATGTCG 7 0.17500000000000002 No Hit GTTGAAGAATGAGCCGGCGACTCATAGGCAGTGGCTTGGTTAAGGGAACG 7 0.17500000000000002 No Hit GAACGCTGGCGGCATGCTTAACACATGCAAGTCGAACGGGAAGTGGTGTT 7 0.17500000000000002 No Hit GCCGCGAATCCTCTTGAAAGAGAGGGGTGCCCTCGGGAACGCGGACACAG 7 0.17500000000000002 No Hit CGGACATTGGTCCTCGAGTGCAAAGGCAGAAGGGAGCTTGACTGCAAGAC 7 0.17500000000000002 No Hit CCCAGGGCTCAACCCTGGACAGGCGGTGGAAACTACCAAGCTGGAGTACG 7 0.17500000000000002 No Hit CTCGTGTTTAGTTGCCACTATGAGTTTGGAACCCTGAACAGACCGCCGGT 7 0.17500000000000002 No Hit CCCAAATAGGTCAACCTTTTAAACTGCCTGCTGAATCCATGAGCAGGCAA 7 0.17500000000000002 No Hit GAAGAAACTTACAAGGATTCCCCTAGTAACGGCGAGCGAACCGGGAGCAG 7 0.17500000000000002 No Hit AGGAGTCTGACATGCGTGCGAGTCGACGGGTTCTGAAACCTGGGATGCGC 7 0.17500000000000002 No Hit GAAACAATGACGGTATCTGAGGAATAAGCATCGGCTAACTCTGTGCCAGC 7 0.17500000000000002 No Hit ATCCTCTTGAAAGAGAGGGGTGCCCTCGGGAACGCGGACACAGGTGGTGC 6 0.15 No Hit GTTGCTAATACCCCGTAGGCTGAGGAGCAAAAGGAGAAATCCGCCCAAGG 6 0.15 No Hit CCTAGCCGTAAACGATGGATACTAGGTGCTGTGCGACTCGACCCGTGCAG 6 0.15 No Hit GTTTGCCTAGAAGCAGCCACCCTTTAAAGAGTGCGTAATAGCTCACTGAT 6 0.15 No Hit CCTAAACCGTGAAAACGGGGTTGTGGGAGAGCAATACAAGCGTTGTGCTG 6 0.15 No Hit ATTGGGCGTAAAGCGTCTGTAGGTGGCTTTTCAAGTCCGCCGTCAAATCC 6 0.15 No Hit CGCGAATCCTCTTGAAAGAGAGGGGTGCCCTCGGGAACGCGGACACAGGT 6 0.15 No Hit GAGTTCGATCCTGGCTCAGGATGAACGCTGGCGGCATGCTTAACACATGC 6 0.15 No Hit CCCAGCTTGAGAATCGGGCGGCCGTGCCGTCCGAATTGTAGTCTGGAGAG 6 0.15 No Hit GATCAGTAGCTGGTCCGAGAGGATGATCAGCCACACTGGGACTGAGACAC 5 0.125 No Hit GGAATTTCCGGTGGAGCGGTGAAATGCATTGAGATCGGAAAGAACACCAA 5 0.125 No Hit ATGGGATTAGAGACCCCAGTAGTCCTAGCCGTAAACGATGGATACTAGGT 5 0.125 No Hit GCTTCATCGTCGAGAGGGAAACAGCCCGGATCACCAGCTAAGGCCCCTAA 5 0.125 No Hit GGAAAGAACACCAACGGCGAAAGCACTCTGCTGGGCCGACACTGACACTG 5 0.125 No Hit CTGAGGAATAAGCATCGGCTAACTCTGTGCCAGCAGCCGCGGTAAGACAG 5 0.125 No Hit GTTTGGAACCCTGAACAGACCGCCGGTGTTAAGCCGGAGGAAGGAGAGGA 5 0.125 No Hit GGTTAAGTCTCGCAACGAGCGCAACCCTCGTGTTTAGTTGCCACTATGAG 5 0.125 No Hit AAACTACCAAGCTGGAGTACGGTAGGGGCAGAGGGAATTTCCGGTGGAGC 5 0.125 No Hit AGCTTACCAAGGCGATGATCAGTAGCTGGTCCGAGAGGATGATCAGCCAC 5 0.125 No Hit CTCGGGAACGCGGACACAGGTGGTGCATGGCTGTCGTCAGCTCGTGCCGT 5 0.125 No Hit CTGACACTGAGAGACGAAAGCTAGGGGAGCAAATGGGATTAGAGACCCCA 5 0.125 No Hit AGGGAATTTCCGGTGGAGCGGTGAAATGCATTGAGATCGGAAAGAACACC 5 0.125 No Hit GTTTGAGGCAATAACAGGTCTGTGATGCCCTTAGATGTTCTGGGCCGCAC 5 0.125 No Hit GTCTGACATGCGTGCGAGTCGACGGGTTCTGAAACCTGGGATGCGCAAGG 5 0.125 No Hit GTGAAAACGGGGTTGTGGGAGAGCAATACAAGCGTTGTGCTGCTAGGCGA 5 0.125 No Hit GGAGTCTGACATGCGTGCGAGTCGACGGGTTCTGAAACCTGGGATGCGCA 5 0.125 No Hit GACAGGTTAGTTTTACCCTACTGATGACCGTGCCGCGATAGTAATTCAAC 5 0.125 No Hit AAGCACTCTGCTGGGCCGACACTGACACTGAGAGACGAAAGCTAGGGGAG 5 0.125 No Hit GATGGTTATAGGTTTAAGGACACAAGGTGACCCTGCTTTTTCAGGGTAAG 5 0.125 No Hit GTTGGGTTAAGTCTCGCAACGAGCGCAACCCTCGTGTTTAGTTGCCACTA 5 0.125 No Hit CCGGGAGCAGCCCAGCTTGAGAATCGGGCGGCCGTGCCGTCCGAATTGTA 5 0.125 No Hit AGTGACCAGGCCCGGGCGACTGTTTACCAAAAACACAGGTCTCCGCAAAG 5 0.125 No Hit GTTAGTTTTACCCTACTGATGACCGTGCCGCGATAGTAATTCAACCTAGT 5 0.125 No Hit GGATGATCAGCCACACTGGGACTGAGACACGGCCCAGACTCCTACGGGAG 5 0.125 No Hit GTTTTACCCTACTGATGACCGTGCCGCGATAGTAATTCAACCTAGTACGA 5 0.125 No Hit CCTGAACAGACCGCCGGTGTTAAGCCGGAGGAAGGAGAGGATGAGGCCAA 5 0.125 No Hit GTGGGATGTCAAAATGCATCGGTAGGGGAGCGTTCCGCCTTAGAGGGAAG 5 0.125 No Hit GTGAAATAGAACGTGAAACCGTGCTGAGCTCCCAAGCAGTGGGAGGGGAA 5 0.125 No Hit GTTGAAGAATCAGCGGATGAGTTGTGGTTAGGGGTGAAATGCCACTCGAA 5 0.125 No Hit GTGCGTAATAGCTCACTGATCGAGCGCCCTTGCGCTGAAGATGAACGGGG 5 0.125 No Hit CCGATAGCGAAGTAGTACCGTGAGGGAAAGGTGAAAAGAACCCCCAGTGG 5 0.125 No Hit GTGGCTTTTCAAGTCCGCCGTCAAATCCCAGGGCTCAACCCTGGACAGGC 5 0.125 No Hit AACCTTACCAGGGCTTGACATGCCGCGAATCCTCTTGAAAGAGAGGGGTG 5 0.125 No Hit CGGGGGTCGACGAAGCGGAAGCGAGAATGTCGGCTTGAGTAACGAAAACA 5 0.125 No Hit CGGGTCGTCAACTTCTTTTCTCGGAGAAGAAACAATGACGGTATCTGAGG 5 0.125 No Hit GTCAGCTCGTGCCGTAAGGTGTTGGGTTAAGTCTCGCAACGAGCGCAACC 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.025 0.0 0.0 0.0 0.0 30-31 0.05 0.0 0.0 0.0 0.0 32-33 0.05 0.0 0.0 0.0 0.0 34-35 0.05 0.0 0.0 0.0 0.0 36-37 0.05 0.0 0.0 0.0 0.0 38-39 0.05 0.0 0.0 0.0 0.0 40-41 0.0625 0.0 0.0 0.0 0.0 42-43 0.1 0.0 0.0 0.0 0.0 44-45 0.125 0.0 0.0 0.0 0.0 46-47 0.1375 0.0 0.0 0.0 0.0 48-49 0.175 0.0 0.0 0.0 0.0 50-51 0.2 0.0 0.0 0.0 0.0 52-53 0.2 0.0 0.0 0.0 0.0 54-55 0.2375 0.0 0.0 0.0 0.0 56-57 0.3 0.0 0.0 0.0 0.0 58-59 0.35 0.0 0.0 0.0 0.0 60-61 0.4125 0.0 0.0 0.0 0.0 62-63 0.5125 0.0 0.0 0.0 0.0 64-65 0.7 0.0 0.0 0.0 0.0 66-67 0.8374999999999999 0.0 0.0 0.0 0.0 68-69 0.8875 0.0 0.0 0.0 0.0 70-71 1.05 0.0 0.0 0.0 0.0 72-73 1.225 0.0 0.0 0.0 0.0 74-75 1.4500000000000002 0.0 0.0 0.0 0.0 76-77 1.6875 0.0 0.0 0.0 0.0 78-79 1.9249999999999998 0.0 0.0 0.0 0.0 80-81 2.2125000000000004 0.0 0.0 0.0 0.0 82-83 2.5875 0.0 0.0 0.0 0.0 84-85 2.975 0.0 0.0 0.0 0.0 86-87 3.45 0.0 0.0 0.0 0.0 88-89 4.125 0.0 0.0 0.0 0.0 90-91 4.9375 0.0 0.0 0.0 0.0 92-93 5.3625 0.0 0.0 0.0 0.0 94-95 6.05 0.0 0.0 0.0 0.0 96-97 6.7125 0.0 0.0 0.0 0.0 98-99 7.4625 0.0 0.0 0.0 0.0 100-101 8.225 0.0 0.0 0.0 0.0 102-103 9.075 0.0 0.0 0.0 0.0 104-105 10.05 0.0 0.0 0.0 0.0 106-107 10.962499999999999 0.0 0.0 0.0 0.0 108-109 11.75 0.0 0.0 0.0 0.0 110-111 12.975000000000001 0.0 0.0 0.0 0.0 112-113 14.2 0.0 0.0 0.0 0.0 114-115 15.2625 0.0 0.0 0.0 0.0 116-117 16.0875 0.0 0.0 0.0 0.0 118-119 17.075 0.0 0.0 0.0 0.0 120-121 17.85 0.0 0.0 0.0 0.0 122-123 18.675 0.0 0.0 0.0 0.0 124-125 19.7 0.0 0.0 0.0 0.0 126-127 20.875 0.0 0.0 0.0 0.0 128-129 21.8125 0.0 0.0 0.0 0.0 130-131 22.8875 0.0 0.0 0.0 0.0 132-133 23.775 0.0 0.0 0.0 0.0 134-135 24.887500000000003 0.0 0.0 0.0 0.0 136-137 26.1875 0.0 0.0 0.0 0.0 138-139 27.174999999999997 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGTGCTA 10 0.006832588 144.9875 5 >>END_MODULE Read 840028 spots for SRR6941546.sra Written 840028 spots for SRR6941546.sra Read 840028 spots for SRR6941546.sra Written 840028 spots for SRR6941546.sra Read 840028 spots for SRR6941546.sra Written 840028 spots for SRR6941546.sra Read 840028 spots for SRR6941546.sra Written 840028 spots for SRR6941546.sra Read 840028 spots for SRR6941546.sra Written 840028 spots for SRR6941546.sra Read 840028 spots for SRR6941546.sra Written 840028 spots for SRR6941546.sra Read 840032 spots for SRR6941546.sra Written 840032 spots for SRR6941546.sra Read 840028 spots for SRR6941546.sra Written 840028 spots for SRR6941546.sra Read 840028 spots for SRR6941546.sra Written 840028 spots for SRR6941546.sra Read 840028 spots for SRR6941546.sra Written 840028 spots for SRR6941546.sra Read 840028 spots for SRR6941546.sra Written 840028 spots for SRR6941546.sra Read 840028 spots for SRR6941546.sra Written 840028 spots for SRR6941546.sra Read 840028 spots for SRR6941546.sra Written 840028 spots for SRR6941546.sra Read 840028 spots for SRR6941546.sra Written 840028 spots for SRR6941546.sra Read 840028 spots for SRR6941546.sra Written 840028 spots for SRR6941546.sra Read 840028 spots for SRR6941546.sra Written 840028 spots for SRR6941546.sra Read 840028 spots for SRR6941546.sra Written 840028 spots for SRR6941546.sra Read 840028 spots for SRR6941546.sra Written 840028 spots for SRR6941546.sra Read 840028 spots for SRR6941546.sra Written 840028 spots for SRR6941546.sra Read 840028 spots for SRR6941546.sra Written 840028 spots for SRR6941546.sra SRR ids: ['SRR6941546.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_0h9sgc25 SRR6941546.sra spots: 16800564 blocks: [[1, 840028], [840029, 1680056], [1680057, 2520084], [2520085, 3360112], [3360113, 4200140], [4200141, 5040168], [5040169, 5880196], [5880197, 6720224], [6720225, 7560252], [7560253, 8400280], [8400281, 9240308], [9240309, 10080336], [10080337, 10920364], [10920365, 11760392], [11760393, 12600420], [12600421, 13440448], [13440449, 14280476], [14280477, 15120504], [15120505, 15960532], [15960533, 16800564]] SRR6941546 file size 5671459 SRR6941546 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR6941546 SRR6941546_1.fastq SRR6941546_2.fastq Input file: SRR6941546_1.fastq Paired file: SRR6941546_2.fastq trimmed: SRR6941546-trimmed-pair1.fastq, SRR6941546-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Fri Dec 6 10:51:14 2024 >> started Fri Dec 6 10:51:34 2024 >> done (20.131s) 16800564 read pairs processed; of these: 4226 ( 0.03%) short read pairs filtered out after trimming by size control 6937 ( 0.04%) empty read pairs filtered out after trimming by size control 16789401 (99.93%) read pairs available; of these: 8076265 (48.10%) trimmed read pairs available after processing 8713136 (51.90%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 56 0.00% 19 107 0.00% 20 56 0.00% 21 104 0.00% 22 100 0.00% 23 115 0.00% 24 139 0.00% 25 173 0.00% 26 230 0.00% 27 248 0.00% 28 301 0.00% 29 370 0.00% 30 418 0.00% 31 483 0.00% 32 595 0.00% 33 630 0.00% 34 648 0.00% 35 755 0.00% 36 825 0.00% 37 777 0.00% 38 926 0.01% 39 1048 0.01% 40 1159 0.01% 41 1283 0.01% 42 1283 0.01% 43 1413 0.01% 44 1506 0.01% 45 1637 0.01% 46 1671 0.01% 47 1796 0.01% 48 1841 0.01% 49 2182 0.01% 50 2330 0.01% 51 2481 0.01% 52 2883 0.02% 53 3222 0.02% 54 3661 0.02% 55 3697 0.02% 56 3831 0.02% 57 4372 0.03% 58 5317 0.03% 59 5318 0.03% 60 5522 0.03% 61 6918 0.04% 62 8089 0.05% 63 7908 0.05% 64 9098 0.05% 65 9981 0.06% 66 10572 0.06% 67 10712 0.06% 68 13308 0.08% 69 14057 0.08% 70 15722 0.09% 71 14917 0.09% 72 19619 0.12% 73 20553 0.12% 74 18760 0.11% 75 20666 0.12% 76 22403 0.13% 77 23988 0.14% 78 25141 0.15% 79 28211 0.17% 80 31141 0.19% 81 31894 0.19% 82 35436 0.21% 83 35931 0.21% 84 37108 0.22% 85 45588 0.27% 86 48857 0.29% 87 50466 0.30% 88 60678 0.36% 89 50377 0.30% 90 51567 0.31% 91 55694 0.33% 92 57921 0.34% 93 62938 0.37% 94 64436 0.38% 95 63378 0.38% 96 64289 0.38% 97 69340 0.41% 98 69917 0.42% 99 72347 0.43% 100 71390 0.43% 101 78166 0.47% 102 83010 0.49% 103 79318 0.47% 104 89324 0.53% 105 80469 0.48% 106 84416 0.50% 107 86954 0.52% 108 82667 0.49% 109 104207 0.62% 110 93611 0.56% 111 97982 0.58% 112 102198 0.61% 113 88032 0.52% 114 86137 0.51% 115 92074 0.55% 116 82790 0.49% 117 86624 0.52% 118 80732 0.48% 119 80885 0.48% 120 94364 0.56% 121 81033 0.48% 122 81888 0.49% 123 103303 0.62% 124 92193 0.55% 125 96909 0.58% 126 84782 0.50% 127 80807 0.48% 128 88134 0.52% 129 85868 0.51% 130 84800 0.51% 131 86485 0.52% 132 88770 0.53% 133 86503 0.52% 134 87547 0.52% 135 88884 0.53% 136 93267 0.56% 137 94664 0.56% 138 95516 0.57% 139 95759 0.57% 140 94210 0.56% 141 93295 0.56% 142 104743 0.62% 143 100778 0.60% 144 106804 0.64% 145 115785 0.69% 146 122773 0.73% 147 139973 0.83% 148 155997 0.93% 149 220966 1.32% 150 1871044 11.14% 151 8713136 51.90% 16789401 reads passed initial QC criterion=sequence-density sequence-density=3.93 sequence-density-rank=1 fanout-score=2.00 fanout-score-rank=27 prefix-density=3.87 prefix-fanout=2.0 sequence=GTATTTAGCCTTG criterion=fanout-score sequence-density=0.01 sequence-density-rank=40 fanout-score=143.50 fanout-score-rank=1 prefix-density=0.91 prefix-fanout=1.0 sequence=CTCGCTTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCATTAATAGCGGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTACGTTCGTGCATTACTTCCATACCAAGATTAGCACGGTTGATGATATCAGCCCAAGTATTAATAACGCGACCTTGACTATCAACTACAGATTGGTTGAAATTGAATCCATTTAGGTTGAACGCCATAGTACTAATACCTAAAGCAGTGAACCAGATTCCTACTACAGGCCAAGCAGCCAAGAAGAAGTGTAAAGAACGAGAGTTGTTGAAACTAGCATATTGGAAGATTAATCGGCCAAAATAACCATGAGCAGCCACAATATTATAAGTCTCTTCCTCTTGACCAAATTTGTAACCCTCATTAGCAGATTCATTTTCAGTAGTTTCCCTGATCAAACTAGAGGTTACCAAGGAACCATGCATAGCACTGAATAGGGAACCGCCGAAAACACCAGCTACACCTAA criterion=sequence-density sequence-density=1.65 sequence-density-rank=1 fanout-score=2.02 fanout-score-rank=26 prefix-density=1.67 prefix-fanout=2.0 sequence=CCTAGTACGAGAGGA criterion=fanout-score sequence-density=0.07 sequence-density-rank=30 fanout-score=30.55 fanout-score-rank=1 prefix-density=2.06 prefix-fanout=1.0 sequence=GCTGCAGCGCTTGGTACTCGGACCTCGGCTCGAGGCATTTTCTCTACCCCTTCTTACCCTGAAAAAGCAGGGTCACCTTGTGTCCTTAAACCTATAACCATCTTTCGGCTAACCTAGCCTCCTCCGTCCCTCCGTACCAACAAGGGGTAGTACAGGAATATTGACCTGTTGTCCATCGACTACGCCTTTCGGCCTGATCTTAGGCCCTGACTCACCCTCCGTGGACGAACCTTGCGGAGGAAACCTTGGGTTTTCGGGGCATTGGATTCTCACCAATGTTTTCGTTACTCAAGCCGACATTCTCGCTTCCGCTTCGTCGACCCCCGCTTTCGCGGTTGCTTCCCTCTAAGGCGGAACGCTCCCCTACCGATGCATTTTGACATCCCACAGCTTCGGCAGATCGCTTAGCCCCGTTCATCTTCAGCGCAAGGGCGCTCGATCAGTGAGCTATTACGCACTCTTTAAAGGGTGGCTGCTTCTAGGCAAACCTCCTGGCTGTCTTTGCACCCCC Potential 3prime adapter identified. Now checking if in reference sequence /dee2/code/volunteer_pipeline.sh: line 905: -f: command not found /dee2/code/volunteer_pipeline.sh: line 906: -f: command not found Adapter seq not found in reference. Now shuffling file before clipping skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -t 20 -x GTATTTAGCCTTG -y CCTAGTACGAGAGGA -o SRR6941546 SRR6941546_1.fastq SRR6941546_2.fastq Input file: SRR6941546_1.fastq Paired file: SRR6941546_2.fastq trimmed: SRR6941546-trimmed-pair1.fastq, SRR6941546-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): GTATTTAGCCTTG -- paired 3' end adapter sequence (-y): CCTAGTACGAGAGGA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Fri Dec 6 10:53:48 2024 >> started Fri Dec 6 10:53:54 2024 >> done (6.736s) 5596467 read pairs processed; of these: 331 ( 0.01%) short read pairs filtered out after trimming by size control 661 ( 0.01%) empty read pairs filtered out after trimming by size control 5595475 (99.98%) read pairs available; of these: 591 ( 0.01%) trimmed read pairs available after processing 5594884 (99.99%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 27 0.00% 19 27 0.00% 20 13 0.00% 21 34 0.00% 22 35 0.00% 23 31 0.00% 24 45 0.00% 25 52 0.00% 26 71 0.00% 27 70 0.00% 28 94 0.00% 29 111 0.00% 30 149 0.00% 31 155 0.00% 32 180 0.00% 33 213 0.00% 34 222 0.00% 35 257 0.00% 36 283 0.01% 37 261 0.00% 38 300 0.01% 39 345 0.01% 40 357 0.01% 41 441 0.01% 42 422 0.01% 43 474 0.01% 44 513 0.01% 45 546 0.01% 46 549 0.01% 47 556 0.01% 48 625 0.01% 49 750 0.01% 50 781 0.01% 51 834 0.01% 52 951 0.02% 53 1089 0.02% 54 1234 0.02% 55 1232 0.02% 56 1275 0.02% 57 1419 0.03% 58 1767 0.03% 59 1793 0.03% 60 1852 0.03% 61 2305 0.04% 62 2679 0.05% 63 2678 0.05% 64 3047 0.05% 65 3378 0.06% 66 3424 0.06% 67 3589 0.06% 68 4555 0.08% 69 4721 0.08% 70 5158 0.09% 71 4930 0.09% 72 6513 0.12% 73 6875 0.12% 74 6193 0.11% 75 6952 0.12% 76 7430 0.13% 77 8022 0.14% 78 8489 0.15% 79 9291 0.17% 80 10346 0.18% 81 10593 0.19% 82 11836 0.21% 83 11747 0.21% 84 12269 0.22% 85 15203 0.27% 86 16172 0.29% 87 16929 0.30% 88 20416 0.36% 89 16718 0.30% 90 17055 0.30% 91 18670 0.33% 92 19394 0.35% 93 21062 0.38% 94 21557 0.39% 95 21179 0.38% 96 21375 0.38% 97 23084 0.41% 98 23164 0.41% 99 24109 0.43% 100 23804 0.43% 101 25967 0.46% 102 27819 0.50% 103 26366 0.47% 104 29890 0.53% 105 26920 0.48% 106 28095 0.50% 107 28902 0.52% 108 27547 0.49% 109 34644 0.62% 110 31322 0.56% 111 32942 0.59% 112 34143 0.61% 113 29378 0.53% 114 28747 0.51% 115 30966 0.55% 116 27398 0.49% 117 28792 0.51% 118 27014 0.48% 119 26635 0.48% 120 31464 0.56% 121 26852 0.48% 122 27379 0.49% 123 34607 0.62% 124 30564 0.55% 125 32150 0.57% 126 28462 0.51% 127 26827 0.48% 128 29769 0.53% 129 28705 0.51% 130 28301 0.51% 131 28596 0.51% 132 29627 0.53% 133 28717 0.51% 134 29225 0.52% 135 29645 0.53% 136 31023 0.55% 137 31616 0.57% 138 31627 0.57% 139 31926 0.57% 140 31526 0.56% 141 30987 0.55% 142 34784 0.62% 143 33535 0.60% 144 35675 0.64% 145 38562 0.69% 146 40672 0.73% 147 46856 0.84% 148 51908 0.93% 149 73623 1.32% 150 622791 11.13% 151 2904641 51.91% criterion=sequence-density sequence-density=3.92 sequence-density-rank=1 fanout-score=2.00 fanout-score-rank=28 prefix-density=3.87 prefix-fanout=2.0 sequence=GTATTTAGCCTTG criterion=fanout-score sequence-density=0.01 sequence-density-rank=40 fanout-score=139.59 fanout-score-rank=1 prefix-density=0.89 prefix-fanout=1.0 sequence=CTCGCTTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCATTAATAGCGGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTACGTTCGTGCATTACTTCCATACCAAGATTAGCACGGTTGATGATATCAGCCCAAGTATTAATAACGCGACCTTGACTATCAACTACAGATTGGTTGAAATTGAATCCATTTAGGTTGAACGCCATAGTACTAATACCTAAAGCAGTGAACCAGATTCCTACTACAGGCCAAGCAGCCAAGAAGAAGTGTAAAGAACGAGAGTTGTTGAAACTAGCATATTGGAAGATTAATCGGCCAAAATAACCATGAGCAGCCACAATATTATAAGTCTCTTCCTCTTGACCAAATTTGTAACCCTCATTAGCAGATTCATTTTCAGTAGTTTCCCTGATCAAACTAGAGGTTACCAAGGAACCATGCATAGCACTGAATAGGGAACCGCCGAAAACACCAGCTACACCTAA criterion=sequence-density sequence-density=1.64 sequence-density-rank=1 fanout-score=2.03 fanout-score-rank=26 prefix-density=1.67 prefix-fanout=2.0 sequence=CCTAGTACGAGAGGA criterion=fanout-score sequence-density=0.07 sequence-density-rank=29 fanout-score=30.41 fanout-score-rank=1 prefix-density=2.05 prefix-fanout=1.0 sequence=GCTGCAGCGCTTGGTACTCGGACCTCGGCTCGAGGCATTTTCTCTACCCCTTCTTACCCTGAAAAAGCAGGGTCACCTTGTGTCCTTAAACCTATAACCATCTTTCGGCTAACCTAGCCTCCTCCGTCCCTCCGTACCAACAAGGGGTAGTACAGGAATATTGACCTGTTGTCCATCGACTACGCCTTTCGGCCTGATCTTAGGCCCTGACTCACCCTCCGTGGACGAACCTTGCGGAGGAAACCTTGGGTTTTCGGGGCATTGGATTCTCACCAATGTTTTCGTTACTCAAGCCGACATTCTCGCTTCCGCTTCGTCGACCCCCGCTTTCGCGGTTGCTTCCCTCTAAGGCGGAACGCTCCCCTACCGATGCATTTTGACATCCCACAGCTTCGGCAGATCGCTTAGCCCCGTTCATCTTCAGCGCAAGGGCGCTCGATCAGTGAGCTATTACGCACTCTTTAAAGGGTGGCTGCTTCTAGGCAAACCTCCTGGCTGTCTTTGCACCCCC SRR6941546 testing PE reads STAR mapping to Ensembl genome Started job on | Dec 06 10:54:43 Started mapping on | Dec 06 10:54:43 Finished on | Dec 06 10:56:05 Mapping speed, Million of reads per hour | 737.05 Number of input reads | 16788409 Average input read length | 276 UNIQUE READS: Uniquely mapped reads number | 4439738 Uniquely mapped reads % | 26.45% Average mapped length | 290.15 Number of splices: Total | 336653 Number of splices: Annotated (sjdb) | 266089 Number of splices: GT/AG | 285168 Number of splices: GC/AG | 4763 Number of splices: AT/AC | 868 Number of splices: Non-canonical | 45854 Mismatch rate per base, % | 0.09% Deletion rate per base | 0.00% Deletion average length | 1.53 Insertion rate per base | 0.00% Insertion average length | 1.65 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 7534037 % of reads mapped to multiple loci | 44.88% Number of reads mapped to too many loci | 1058577 % of reads mapped to too many loci | 6.31% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 2.14% % of reads unmapped: other | 20.23% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 4816726 4816726 4816726 N_multimapping 7534037 7534037 7534037 N_noFeature 3712203 4373067 3759483 N_ambiguous 45504 2797 24643 UnstrandedReadsAssigned:682031 PositiveStrandReadsAssigned:63874 NegativeStrandReadsAssigned:655612 Dataset is classified negative stranded MeadianReadLen=151 20thPercentileLength=122 echo kmer=117 SRR6941546 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,972 [index] number of k-mers: 66,720,672 [index] number of equivalence classes: 111,837 [quant] running in paired-end mode [quant] will process pair 1: SRR6941546-trimmed-pair1.fastq SRR6941546-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 16,788,409 reads, 3,031,517 reads pseudoaligned [quant] estimated average fragment length: 166.519 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 889 rounds 52973 SRR6941546.ke.tsv 35125 SRR6941546.se.tsv 88098 total ==> SRR6941546.ke.tsv <== target_id length eff_length est_counts tpm PNS24245 936 770.598 0.446191 0.0426311 PNS24247 1044 878.481 0 0 PNS24249 1928 1762.48 3.55381 0.148458 PNS24246 1044 878.481 0 0 PNS24248 1044 878.481 0 0 PNS24244 1471 1305.48 0 0 PNS24243 293 133.169 0 0 KQK14069 1603 1437.48 193.526 9.91223 KQK14071 474 309.222 5.1444 1.22489 ==> SRR6941546.se.tsv <== BRADI_1g14170v3 231 BRADI_1g53295v3 2 BRADI_1g59795v3 2 BRADI_1g07683v3 0 BRADI_1g00485v3 0 BRADI_1g20270v3 13 BRADI_1g74790v3 4 BRADI_1g09890v3 0 BRADI_1g77505v3 1 BRADI_1g48960v3 0 SRR6941546 completed mapping pipeline successfully