Starting /dee2/code/volunteer_pipeline.sh SRR6941547 current disk space = 1550894288896 free memory = 1597659956 SRR6941547 SRAfilesize 6cf0e990e6c6e6736fc53b0c0c842eef SRR6941547.sra SRR6941547.sra file validated SRR6941547 is paired end SRR6941547 is conventional basespace SRR6941547 read1 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR6941547_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 34.64525 35.0 35.0 35.0 35.0 35.0 2 34.611 35.0 35.0 35.0 35.0 35.0 3 34.638 35.0 35.0 35.0 35.0 35.0 4 34.5625 35.0 35.0 35.0 34.0 35.0 5 34.61625 35.0 35.0 35.0 35.0 35.0 6 39.41675 40.0 40.0 40.0 39.0 40.0 7 39.424 40.0 40.0 40.0 39.0 40.0 8 39.18525 40.0 40.0 40.0 39.0 40.0 9 39.41725 40.0 40.0 40.0 39.0 40.0 10-14 39.42110000000001 40.0 40.0 40.0 39.0 40.0 15-19 39.4272 40.0 40.0 40.0 39.0 40.0 20-24 39.34095000000001 40.0 40.0 40.0 39.0 40.0 25-29 39.34310000000001 40.0 40.0 40.0 39.0 40.0 30-34 39.37445 40.0 40.0 40.0 39.0 40.0 35-39 39.33135 40.0 40.0 40.0 39.0 40.0 40-44 39.37015 40.0 40.0 40.0 39.0 40.0 45-49 39.3386 40.0 40.0 40.0 39.0 40.0 50-54 39.2881 40.0 40.0 40.0 39.0 40.0 55-59 39.269149999999996 40.0 40.0 40.0 39.0 40.0 60-64 39.3113 40.0 40.0 40.0 39.0 40.0 65-69 39.26735 40.0 40.0 40.0 39.0 40.0 70-74 39.25655 40.0 40.0 40.0 39.0 40.0 75-79 39.1936 40.0 40.0 40.0 38.8 40.0 80-84 39.18605 40.0 40.0 40.0 38.8 40.0 85-89 39.136849999999995 40.0 40.0 40.0 39.0 40.0 90-94 39.2057 40.0 40.0 40.0 39.0 40.0 95-99 39.023450000000004 40.0 39.8 40.0 38.4 40.0 100-104 38.2729 39.4 38.6 39.6 36.6 39.8 105-109 38.99585 40.0 39.6 40.0 38.2 40.0 110-114 39.1276 40.0 40.0 40.0 38.8 40.0 115-119 39.17095 40.0 40.0 40.0 39.0 40.0 120-124 39.095200000000006 40.0 40.0 40.0 38.6 40.0 125-129 38.989250000000006 40.0 40.0 40.0 38.0 40.0 130-134 38.9416 40.0 39.8 40.0 37.8 40.0 135-139 38.7077 40.0 39.0 40.0 36.8 40.0 140-144 38.7382 40.0 39.0 40.0 37.4 40.0 145-149 38.61985 40.0 39.0 40.0 36.8 40.0 150-151 37.320375 39.5 37.5 40.0 33.5 40.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 18 1.0 19 2.0 20 1.0 21 0.0 22 1.0 23 0.0 24 1.0 25 2.0 26 6.0 27 12.0 28 13.0 29 15.0 30 22.0 31 28.0 32 29.0 33 40.0 34 52.0 35 58.0 36 93.0 37 130.0 38 272.0 39 3222.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 57.51503006012024 11.047094188376754 4.584168336673346 26.853707414829657 2 22.15 12.85 30.825000000000003 34.175 3 22.175 19.950000000000003 26.85 31.025000000000002 4 25.8 26.150000000000002 22.15 25.900000000000002 5 24.525 34.625 21.825 19.025 6 19.75 38.025 21.325 20.9 7 14.75 31.025000000000002 39.45 14.774999999999999 8 16.8 28.275 30.825000000000003 24.099999999999998 9 16.75 23.9 37.175000000000004 22.175 10-14 19.475 34.185 24.815 21.525 15-19 20.25 31.525 25.165 23.06 20-24 18.292743911586737 31.80477071560734 27.234085112766916 22.668400260039007 25-29 21.52 31.580000000000002 26.69 20.21 30-34 22.195 32.68 24.515 20.61 35-39 21.375 30.049999999999997 26.875 21.7 40-44 19.21884376875375 30.441088217643532 26.6753350670134 23.664732946589318 45-49 19.27 30.235 28.375 22.12 50-54 20.94 30.675 26.025 22.36 55-59 19.975 29.925 26.900000000000002 23.200000000000003 60-64 18.96 30.36 27.034999999999997 23.645 65-69 19.564999999999998 31.09 26.150000000000002 23.195 70-74 20.044999999999998 31.09 24.310000000000002 24.555 75-79 20.625 29.854999999999997 26.88 22.64 80-84 21.665 30.009999999999998 25.729999999999997 22.595000000000002 85-89 21.98 29.48 27.0 21.54 90-94 19.009999999999998 31.175000000000004 26.3 23.515 95-99 20.235 31.41 23.995 24.36 100-104 19.975 32.629999999999995 24.785 22.61 105-109 19.53 31.319999999999997 26.724999999999998 22.425 110-114 21.01 29.505 25.75 23.735 115-119 19.935 32.035000000000004 23.68 24.349999999999998 120-124 20.330000000000002 30.099999999999998 23.974999999999998 25.595000000000002 125-129 19.875 30.490000000000002 25.255 24.38 130-134 21.385 31.405 24.13 23.080000000000002 135-139 22.075 30.785 25.05 22.09 140-144 22.955000000000002 29.82 25.669999999999998 21.555 145-149 20.195 30.945 25.230000000000004 23.630000000000003 150-151 20.1 32.8375 22.662499999999998 24.4 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.5 4 1.0 5 1.0 6 0.5 7 0.0 8 0.0 9 0.5 10 0.5 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 1.0 18 1.0 19 0.0 20 1.0 21 2.5 22 4.0 23 5.0 24 5.5 25 3.5 26 4.5 27 11.0 28 15.5 29 18.5 30 22.5 31 23.0 32 22.0 33 30.5 34 40.0 35 46.5 36 98.0 37 203.5 38 264.0 39 276.0 40 285.0 41 310.0 42 260.0 43 239.0 44 252.0 45 225.0 46 197.5 47 161.5 48 142.0 49 92.5 50 64.0 51 56.5 52 49.5 53 51.5 54 59.5 55 75.5 56 76.5 57 51.0 58 49.0 59 43.0 60 33.0 61 34.0 62 22.0 63 12.5 64 12.0 65 11.5 66 9.0 67 6.0 68 3.5 69 4.5 70 3.5 71 2.0 72 1.0 73 0.0 74 0.5 75 0.5 76 0.0 77 0.0 78 0.0 79 0.0 80 0.5 81 0.5 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.015 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.02 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.62499999999999 #Duplication Level Percentage of deduplicated Percentage of total 1 82.96907216494846 50.3 2 8.206185567010309 9.950000000000001 3 3.0927835051546393 5.625 4 1.402061855670103 3.4000000000000004 5 1.1958762886597938 3.6249999999999996 6 0.49484536082474223 1.7999999999999998 7 0.4536082474226804 1.925 8 0.2061855670103093 1.0 9 0.3711340206185567 2.025 >10 1.5257731958762888 17.075000000000003 >50 0.08247422680412371 3.2750000000000004 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GCGGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTACG 72 1.7999999999999998 No Hit GGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTACGTT 59 1.4749999999999999 No Hit GGCCAAAATAACCATGAGCAGCCACAATATTATAAGTCTCTTCCTCTTGA 45 1.125 No Hit GCTTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCA 39 0.975 No Hit GCTCTGCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTCCTAAA 34 0.8500000000000001 No Hit GTGCTCTGCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTCCTA 33 0.8250000000000001 No Hit GCCAAAATAACCATGAGCAGCCACAATATTATAAGTCTCTTCCTCTTGAC 31 0.775 No Hit GCGGAAAAACCTTATCCATTAATAGCGGGAACTTCAAGAGCAGCTAGATC 27 0.675 No Hit GTTGAAACTAGCATATTGGAAGATTAATCGGCCAAAATAACCATGAGCAG 26 0.65 No Hit CTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCATTAAT 24 0.6 No Hit GCCGAAAACACCAGCTACACCTAACATGTGAAATGGATGCATAAGGATGT 24 0.6 No Hit GTTAGCGGAAAAACCTTATCCATTAATAGCGGGAACTTCAAGAGCAGCTA 23 0.575 No Hit GCGACCTTGACTATCAACTACAGATTGGTTGAAATTGAATCCATTTAGGT 20 0.5 No Hit CTTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCAT 20 0.5 No Hit GCATAAGGATGTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAAAGTA 18 0.44999999999999996 No Hit CGGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTACGT 17 0.42500000000000004 No Hit CTCATAAGGACCACCATTGTATAACCATTCATCAACGGATGCAGCTTCCC 17 0.42500000000000004 No Hit GGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTACGTTC 17 0.42500000000000004 No Hit GTTGATGATATCAGCCCAAGTATTAATAACGCGACCTTGACTATCAACTA 16 0.4 No Hit GCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTCCTAAAGGCAT 16 0.4 No Hit GTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTC 15 0.375 No Hit GGTCAATAAGGTAGGGATCATCAAAACACCGAACCATCCGATGTAAAGAC 15 0.375 No Hit GTTGAAATTGAATCCATTTAGGTTGAACGCCATAGTACTAATACCTAAAG 15 0.375 No Hit GCGGTCAATAAGGTAGGGATCATCAAAACACCGAACCATCCGATGTAAAG 14 0.35000000000000003 No Hit GGATGCATAAGGATGTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAA 14 0.35000000000000003 No Hit GGCGATAATAAATACAGAAGTTGCGGTCAATAAGGTAGGGATCATCAAAA 13 0.325 No Hit GTGAGCATTACGTTCGTGCATTACTTCCATACCAAGATTAGCACGGTTGA 13 0.325 No Hit CCACAATATTATAAGTCTCTTCCTCTTGACCAAATTTGTAACCCTCATTA 13 0.325 No Hit GCTAGATCTAGAGGGAAGTTGTGAGCATTACGTTCGTGCATTACTTCCAT 13 0.325 No Hit GGAAAAACCTTATCCATTAATAGCGGGAACTTCAAGAGCAGCTAGATCTA 12 0.3 No Hit GGTAAATCAAGAAAACAGCAGTCGCAGCTGCAACAGGAGCTGAATATGCA 12 0.3 No Hit GGGGCTGCGATGAAGGCGATAATAAATACAGAAGTTGCGGTCAATAAGGT 12 0.3 No Hit GCCAAGAAGAAGTGTAAAGAACGAGAGTTGTTGAAACTAGCATATTGGAA 12 0.3 No Hit GCACGGTTGATGATATCAGCCCAAGTATTAATAACGCGACCTTGACTATC 11 0.27499999999999997 No Hit GGAGGGGCTGCGATGAAGGCGATAATAAATACAGAAGTTGCGGTCAATAA 11 0.27499999999999997 No Hit CTCTTGACCAAATTTGTAACCCTCATTAGCAGATTCATTTTCAGTAGTTT 11 0.27499999999999997 No Hit CCACCATTGTATAACCATTCATCAACGGATGCAGCTTCCCAAATTGGGTA 10 0.25 No Hit ACGCGACCTTGACTATCAACTACAGATTGGTTGAAATTGAATCCATTTAG 10 0.25 No Hit GATGCATAAGGATGTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAAA 10 0.25 No Hit GGTTGATGATATCAGCCCAAGTATTAATAACGCGACCTTGACTATCAACT 9 0.22499999999999998 No Hit TTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCATT 9 0.22499999999999998 No Hit CCCAAGTATTAATAACGCGACCTTGACTATCAACTACAGATTGGTTGAAA 9 0.22499999999999998 No Hit CCAGATTCCTACTACAGGCCAAGCAGCCAAGAAGAAGTGTAAAGAACGAG 9 0.22499999999999998 No Hit GCTAGTTATCCAGTTGCAGAAGCGACCCCACAGGCTTGTACTTTCGCGTC 9 0.22499999999999998 No Hit GCTGCGATGAAGGCGATAATAAATACAGAAGTTGCGGTCAATAAGGTAGG 9 0.22499999999999998 No Hit CTCTGCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTCCTAAAG 9 0.22499999999999998 No Hit CACCATTGTATAACCATTCATCAACGGATGCAGCTTCCCAAATTGGGTAA 9 0.22499999999999998 No Hit CCTCATTAGCAGATTCATTTTCAGTAGTTTCCCTGATCAAACTAGAGGTT 9 0.22499999999999998 No Hit CTGCGATGAAGGCGATAATAAATACAGAAGTTGCGGTCAATAAGGTAGGG 8 0.2 No Hit CTTGACTATCAACTACAGATTGGTTGAAATTGAATCCATTTAGGTTGAAC 8 0.2 No Hit ACCAGATATTCCTAAAGGCATACCATCAGAGAAGCTTCCTTGACCAATAG 8 0.2 No Hit GTCGCAGCTGCAACAGGAGCTGAATATGCAACAGCAATCCAAGGGCGCAT 8 0.2 No Hit GAGTTGTTGAAACTAGCATATTGGAAGATTAATCGGCCAAAATAACCATG 8 0.2 No Hit GGCGCATACCCAAACGGAAACTAAGTTCCCACTCACGACCCATATAACAA 7 0.17500000000000002 No Hit GTCTCTTCCTCTTGACCAAATTTGTAACCCTCATTAGCAGATTCATTTTC 7 0.17500000000000002 No Hit GGGAAGTTGTGAGCATTACGTTCGTGCATTACTTCCATACCAAGATTAGC 7 0.17500000000000002 No Hit GCATTACGTTCGTGCATTACTTCCATACCAAGATTAGCACGGTTGATGAT 7 0.17500000000000002 No Hit GTAGGGATCATCAAAACACCGAACCATCCGATGTAAAGACGATTTTCAGT 7 0.17500000000000002 No Hit GTAAAGAACGAGAGTTGTTGAAACTAGCATATTGGAAGATTAATCGGCCA 7 0.17500000000000002 No Hit CTAGCATATTGGAAGATTAATCGGCCAAAATAACCATGAGCAGCCACAAT 7 0.17500000000000002 No Hit ATATAACAAGCTACACCAAGTAAGAAGTGTAGAACAATTAGCTCATAAGG 7 0.17500000000000002 No Hit GCCCAAGTATTAATAACGCGACCTTGACTATCAACTACAGATTGGTTGAA 7 0.17500000000000002 No Hit GTCCATGTACCAGTAGAAGATTCGGCAGCTACTGCAGCCCCTGCTTCTTC 7 0.17500000000000002 No Hit GACCCATATAACAAGCTACACCAAGTAAGAAGTGTAGAACAATTAGCTCA 7 0.17500000000000002 No Hit GCGATGAAGGCGATAATAAATACAGAAGTTGCGGTCAATAAGGTAGGGAT 6 0.15 No Hit GACCACCATTGTATAACCATTCATCAACGGATGCAGCTTCCCAAATTGGG 6 0.15 No Hit CAGTGAACCAGATTCCTACTACAGGCCAAGCAGCCAAGAAGAAGTGTAAA 6 0.15 No Hit CAAGTATTAATAACGCGACCTTGACTATCAACTACAGATTGGTTGAAATT 6 0.15 No Hit TGATGATATCAGCCCAAGTATTAATAACGCGACCTTGACTATCAACTACA 6 0.15 No Hit GGGCTGCGATGAAGGCGATAATAAATACAGAAGTTGCGGTCAATAAGGTA 6 0.15 No Hit CTACAGGCCAAGCAGCCAAGAAGAAGTGTAAAGAACGAGAGTTGTTGAAA 6 0.15 No Hit GGCCAAGCAGCCAAGAAGAAGTGTAAAGAACGAGAGTTGTTGAAACTAGC 6 0.15 No Hit GCTTTCTTTTCCTCTGGCTACTAAGATGTTTCAGTTCGCCAGGTTGTCTC 6 0.15 No Hit CATAAGGATGTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAAAGTAC 6 0.15 No Hit CTCATTAGCAGATTCATTTTCAGTAGTTTCCCTGATCAAACTAGAGGTTA 6 0.15 No Hit CGCGACCTTGACTATCAACTACAGATTGGTTGAAATTGAATCCATTTAGG 6 0.15 No Hit AGAACAATTAGCTCATAAGGACCACCATTGTATAACCATTCATCAACGGA 5 0.125 No Hit GGGCGCATACCCAAACGGAAACTAAGTTCCCACTCACGACCCATATAACA 5 0.125 No Hit ACCAGCTACACCTAACATGTGAAATGGATGCATAAGGATGTTGTGCTCTG 5 0.125 No Hit AAAAATTCTTATATGTTAGCGGAAAAACCTTATCCATTAATAGCGGGAAC 5 0.125 No Hit ACCATGAGCAGCCACAATATTATAAGTCTCTTCCTCTTGACCAAATTTGT 5 0.125 No Hit GGCATGATGACTTGGCCTCATCCTCTCCTTCCTCCGGCTTAACACCGGCG 5 0.125 No Hit GCTGAATATGCAACAGCAATCCAAGGGCGCATACCCAAACGGAAACTAAG 5 0.125 No Hit TCTGCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTCCTAAAGG 5 0.125 No Hit ATAAGGACCACCATTGTATAACCATTCATCAACGGATGCAGCTTCCCAAA 5 0.125 No Hit GTTGCGGTCAATAAGGTAGGGATCATCAAAACACCGAACCATCCGATGTA 5 0.125 No Hit GCCATAGTACTAATACCTAAAGCAGTGAACCAGATTCCTACTACAGGCCA 5 0.125 No Hit GCACTGAATAGGGAACCGCCGAAAACACCAGCTACACCTAACATGTGAAA 5 0.125 No Hit GATGATATCAGCCCAAGTATTAATAACGCGACCTTGACTATCAACTACAG 5 0.125 No Hit AGGAATAATAGCACCAGAGATAATATTGTTTCCATAAAGTAAAGAACCAG 5 0.125 No Hit GTTGTTGAAACTAGCATATTGGAAGATTAATCGGCCAAAATAACCATGAG 5 0.125 No Hit CCCTCATTAGCAGATTCATTTTCAGTAGTTTCCCTGATCAAACTAGAGGT 5 0.125 No Hit GGATGTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAAAGTACCAGAT 5 0.125 No Hit TAGGAATAATAGCACCAGAGATAATATTGTTTCCATAAAGTAAAGAACCA 5 0.125 No Hit CCAAGTATTAATAACGCGACCTTGACTATCAACTACAGATTGGTTGAAAT 5 0.125 No Hit GTTTCCATAAAGTAAAGAACCAGAAACAGGCTCGCGAATACCATCAATAT 5 0.125 No Hit ACCAGAGATAATATTGTTTCCATAAAGTAAAGAACCAGAAACAGGCTCGC 5 0.125 No Hit GTGCTAGTTATCCAGTTGCAGAAGCGACCCCACAGGCTTGTACTTTCGCG 5 0.125 No Hit GTAGGAATAATAGCACCAGAGATAATATTGTTTCCATAAAGTAAAGAACC 5 0.125 No Hit AGTTGTTGAAACTAGCATATTGGAAGATTAATCGGCCAAAATAACCATGA 5 0.125 No Hit GTTCGTGCATTACTTCCATACCAAGATTAGCACGGTTGATGATATCAGCC 5 0.125 No Hit CCACAGGCTTGTACTTTCGCGTCTCTCTAAAATTGCAGTCATGGTAAGAT 5 0.125 No Hit GTCAATAAGGTAGGGATCATCAAAACACCGAACCATCCGATGTAAAGACG 5 0.125 No Hit CCCCACTGCTGCCTCCCGTAGGAGTCTGGGCCGTGTCTCAGTCCCAGTGT 5 0.125 No Hit CTCGCGAATACCATCAATATCTACTGGAGGGGCTGCGATGAAGGCGATAA 5 0.125 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0125 0.0 0.0 0.0 0.0 44-45 0.025 0.0 0.0 0.0 0.0 46-47 0.025 0.0 0.0 0.0 0.0 48-49 0.025 0.0 0.0 0.0 0.0 50-51 0.025 0.0 0.0 0.0 0.0 52-53 0.025 0.0 0.0 0.0 0.0 54-55 0.025 0.0 0.0 0.0 0.0 56-57 0.025 0.0 0.0 0.0 0.0 58-59 0.1125 0.0 0.0 0.0 0.0 60-61 0.125 0.0 0.0 0.0 0.0 62-63 0.125 0.0 0.0 0.0 0.0 64-65 0.1375 0.0 0.0 0.0 0.0 66-67 0.175 0.0 0.0 0.0 0.0 68-69 0.21250000000000002 0.0 0.0 0.0 0.0 70-71 0.275 0.0 0.0 0.0 0.0 72-73 0.325 0.0 0.0 0.0 0.0 74-75 0.375 0.0 0.0 0.0 0.0 76-77 0.4125 0.0 0.0 0.0 0.0 78-79 0.4375 0.0 0.0 0.0 0.0 80-81 0.5625 0.0 0.0 0.0 0.0 82-83 0.6375 0.0 0.0 0.0 0.0 84-85 0.7625 0.0 0.0 0.0 0.0 86-87 0.8625 0.0 0.0 0.0 0.0 88-89 0.925 0.0 0.0 0.0 0.0 90-91 1.1375000000000002 0.0 0.0 0.0 0.0 92-93 1.325 0.0 0.0 0.0 0.0 94-95 1.5375 0.0 0.0 0.0 0.0 96-97 1.8 0.0 0.0 0.0 0.0 98-99 1.9625 0.0 0.0 0.0 0.0 100-101 2.1625 0.0 0.0 0.0 0.0 102-103 2.4375 0.0 0.0 0.0 0.0 104-105 2.7249999999999996 0.0 0.0 0.0 0.0 106-107 3.0625 0.0 0.0 0.0 0.0 108-109 3.4749999999999996 0.0 0.0 0.0 0.0 110-111 3.8125 0.0 0.0 0.0 0.0 112-113 4.1375 0.0 0.0 0.0 0.0 114-115 4.575 0.0 0.0 0.0 0.0 116-117 4.85 0.0 0.0 0.0 0.0 118-119 5.15 0.0 0.0 0.0 0.0 120-121 5.475 0.0 0.0 0.0 0.0 122-123 5.7875 0.0 0.0 0.0 0.0 124-125 6.137499999999999 0.0 0.0 0.0 0.0 126-127 6.525 0.0 0.0 0.0 0.0 128-129 6.8875 0.0 0.0 0.0 0.0 130-131 7.2875 0.0 0.0 0.0 0.0 132-133 7.7875 0.0 0.0 0.0 0.0 134-135 8.075 0.0 0.0 0.0 0.0 136-137 8.537500000000001 0.0 0.0 0.0 0.0 138-139 9.024999999999999 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content pass >>END_MODULE SRR6941547 read2 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR6941547_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 34.15425 35.0 35.0 35.0 33.0 35.0 2 34.1255 35.0 35.0 35.0 33.0 35.0 3 33.88275 35.0 35.0 35.0 32.0 35.0 4 34.2005 35.0 35.0 35.0 33.0 35.0 5 34.2415 35.0 35.0 35.0 33.0 35.0 6 38.742 40.0 40.0 40.0 38.0 40.0 7 38.9965 40.0 40.0 40.0 39.0 40.0 8 38.95525 40.0 40.0 40.0 39.0 40.0 9 39.00375 40.0 40.0 40.0 39.0 40.0 10-14 38.8976 40.0 40.0 40.0 38.0 40.0 15-19 38.626799999999996 40.0 39.8 40.0 36.8 40.0 20-24 38.961749999999995 40.0 40.0 40.0 38.6 40.0 25-29 38.91109999999999 40.0 40.0 40.0 38.4 40.0 30-34 38.8668 40.0 40.0 40.0 38.2 40.0 35-39 38.82465 40.0 40.0 40.0 37.8 40.0 40-44 38.93435 40.0 40.0 40.0 38.4 40.0 45-49 38.6967 40.0 39.8 40.0 37.2 40.0 50-54 38.7824 40.0 40.0 40.0 37.6 40.0 55-59 38.737649999999995 40.0 39.6 40.0 37.2 40.0 60-64 38.8071 40.0 40.0 40.0 37.8 40.0 65-69 38.7353 40.0 39.8 40.0 37.4 40.0 70-74 38.516000000000005 40.0 39.4 40.0 36.6 40.0 75-79 38.7439 40.0 39.8 40.0 37.6 40.0 80-84 38.7329 40.0 40.0 40.0 37.6 40.0 85-89 38.4305 40.0 39.0 40.0 36.4 40.0 90-94 38.423350000000006 40.0 39.0 40.0 36.0 40.0 95-99 38.25695 40.0 39.0 40.0 35.8 40.0 100-104 37.1367 38.6 37.8 39.4 33.4 39.6 105-109 38.0439 40.0 39.0 40.0 35.4 40.0 110-114 38.0309 40.0 39.0 40.0 35.4 40.0 115-119 37.82695 40.0 39.0 40.0 34.2 40.0 120-124 37.84975000000001 40.0 39.0 40.0 34.8 40.0 125-129 37.5244 40.0 38.8 40.0 33.6 40.0 130-134 37.839 40.0 39.0 40.0 34.8 40.0 135-139 37.55989999999999 40.0 39.0 40.0 33.8 40.0 140-144 37.381299999999996 40.0 39.0 40.0 34.0 40.0 145-149 36.72995 40.0 38.8 40.0 30.8 40.0 150-151 34.660375 38.5 35.0 39.5 23.5 40.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 8.0 3 0.0 4 2.0 5 2.0 6 0.0 7 0.0 8 0.0 9 2.0 10 0.0 11 1.0 12 1.0 13 1.0 14 3.0 15 3.0 16 4.0 17 4.0 18 1.0 19 3.0 20 7.0 21 7.0 22 5.0 23 7.0 24 12.0 25 12.0 26 18.0 27 27.0 28 18.0 29 37.0 30 33.0 31 39.0 32 41.0 33 49.0 34 81.0 35 81.0 36 106.0 37 181.0 38 347.0 39 2857.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 49.761126477244154 20.995725421171738 10.56072416394267 18.68242393764144 2 24.818250188017046 21.559288042115817 34.695412384056155 18.927049385810978 3 19.344344344344343 23.773773773773772 40.590590590590594 16.29129129129129 4 22.685069008782936 29.661229611041406 27.40276035131744 20.250941028858218 5 26.177354709418836 31.012024048096194 26.077154308617235 16.733466933867735 6 20.701754385964914 35.31328320802005 27.142857142857142 16.842105263157894 7 16.808617234468937 21.54308617234469 43.48697394789579 18.161322645290582 8 20.295295295295297 24.724724724724727 30.53053053053053 24.44944944944945 9 22.64764764764765 21.896896896896898 33.48348348348348 21.97197197197197 10-14 23.321484003404596 26.09022179942923 31.502528413358032 19.085765783808142 15-19 22.403166808638574 25.269329057473566 32.12406674349852 20.203437390389336 20-24 23.000300330363398 24.536990689758735 32.670938031835014 19.791770948042846 25-29 22.847416900280336 26.331597917501 31.032238686423707 19.788746495794953 30-34 23.473473473473476 25.205205205205207 31.801801801801805 19.51951951951952 35-39 24.216638302132345 24.041445590149163 31.57973771148263 20.16217839623586 40-44 24.026429071979177 25.167684452898186 30.688757633396733 20.117128841725897 45-49 22.768907352720355 25.701987086440763 31.548125531808395 19.980980029030484 50-54 23.17665315112379 25.108875206487465 31.581318516293738 20.13315312609501 55-59 22.78392311927524 26.61294359077031 29.9914910656189 20.61164222433555 60-64 22.96796796796797 25.22022022022022 31.076076076076077 20.735735735735737 65-69 22.87787787787788 25.335335335335333 30.57057057057057 21.216216216216218 70-74 23.60860860860861 25.25025025025025 31.156156156156158 19.984984984984987 75-79 23.6910601661828 25.608168985884472 30.473520872960258 20.22724997497247 80-84 23.67867867867868 24.67967967967968 32.47747747747748 19.164164164164163 85-89 24.83983983983984 25.585585585585584 29.93993993993994 19.634634634634633 90-94 23.783540248297957 25.575690828994794 30.15118141770124 20.489587505006007 95-99 23.52617355620058 24.712241016915222 31.17305575017516 20.58852967670904 100-104 23.847233360064156 26.082598235765836 30.63352044907779 19.43664795509222 105-109 24.40004016467517 24.229340295210363 31.398734812732204 19.971884727382267 110-114 24.416562107904642 25.1693851944793 30.925972396486827 19.488080301129234 115-119 23.97311800993029 25.442599929785846 30.949395656753097 19.634886403530768 120-124 23.734177215189874 26.476793248945146 28.94816154309825 20.840867992766725 125-129 24.209312816400182 26.43476517467796 29.647636709939352 19.708285298982506 130-134 23.54741595584546 26.537882589061716 29.86954340190667 20.045158053186153 135-139 23.82363280269171 25.952895093657407 30.517752222166422 19.705719881484455 140-144 25.09423531185606 26.265266120520682 29.883902095793335 18.756596471829923 145-149 24.673694779116463 25.411646586345384 31.09437751004016 18.820281124497992 150-151 24.698946312092325 25.614651279478174 31.13396889111892 18.552433517310586 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 3.0 1 2.0 2 1.0 3 0.5 4 0.0 5 0.0 6 0.0 7 0.5 8 0.5 9 0.0 10 0.0 11 0.5 12 1.0 13 0.5 14 0.0 15 0.0 16 0.0 17 0.0 18 0.5 19 1.0 20 1.0 21 1.5 22 3.5 23 3.0 24 3.5 25 10.5 26 14.0 27 16.0 28 23.5 29 28.0 30 30.0 31 31.5 32 31.5 33 44.5 34 69.5 35 85.5 36 109.0 37 168.0 38 210.5 39 225.5 40 257.0 41 270.5 42 224.0 43 228.0 44 267.0 45 230.5 46 206.0 47 171.5 48 128.0 49 98.5 50 68.0 51 70.5 52 61.0 53 53.5 54 69.0 55 77.5 56 74.0 57 59.0 58 47.0 59 39.5 60 35.5 61 34.5 62 27.5 63 18.5 64 13.5 65 11.0 66 6.0 67 7.0 68 8.5 69 8.5 70 6.0 71 1.0 72 1.0 73 1.0 74 0.5 75 0.5 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.575 2 0.27499999999999997 3 0.1 4 0.375 5 0.2 6 0.25 7 0.2 8 0.1 9 0.1 10-14 0.135 15-19 0.215 20-24 0.11 25-29 0.12 30-34 0.1 35-39 0.11 40-44 0.11 45-49 0.105 50-54 0.11499999999999999 55-59 0.105 60-64 0.1 65-69 0.1 70-74 0.1 75-79 0.11 80-84 0.1 85-89 0.1 90-94 0.12 95-99 0.09 100-104 0.24 105-109 0.41000000000000003 110-114 0.375 115-119 0.305 120-124 0.45999999999999996 125-129 0.245 130-134 0.35000000000000003 135-139 0.43499999999999994 140-144 0.515 145-149 0.4 150-151 0.35000000000000003 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 63.575 #Duplication Level Percentage of deduplicated Percentage of total 1 82.22571765631145 52.275000000000006 2 8.061344868265827 10.25 3 3.3031852143138023 6.3 4 1.730239874164373 4.3999999999999995 5 1.376327172630751 4.375 6 0.7864726700747149 3.0 7 0.47188360204482893 2.1 8 0.3539127015336217 1.7999999999999998 9 0.15729453401494298 0.8999999999999999 >10 1.533621706645694 14.6 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GCTAGTTTCAACAACTCTCGTTCTTTACACTTCTTCTTGGCTGCTTGGCC 29 0.7250000000000001 No Hit GCTTGTTATATGGGTCGTGAGTGGGAACTTAGTTTCCGTTTGGGTATGCG 26 0.65 No Hit ATTATCTATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAAT 24 0.6 No Hit GCACAACATCCTTATGCATCCATTTCACATGTTAGGTGTAGCTGGTGTTT 23 0.575 No Hit GTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGATCTTACCATGAC 22 0.5499999999999999 No Hit GTTTCAACAACTCTCGTTCTTTACACTTCTTCTTGGCTGCTTGGCCTGTA 20 0.5 No Hit GTTATTTTGGCCGATTAATCTTCCAATATGCTAGTTTCAACAACTCTCGT 19 0.475 No Hit GCTGCGACTGCTGTTTTCTTGATTTACCCTATTGGTCAAGGAAGCTTCTC 19 0.475 No Hit TCTATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGA 19 0.475 No Hit GTTTTGATGATCCCTACCTTATTGACCGCAACTTCTGTATTTATTATCGC 16 0.4 No Hit GTTATACAATGGTGGTCCTTATGAGCTAATTGTTCTACACTTCTTACTTG 16 0.4 No Hit GTGGGAACTTAGTTTCCGTTTGGGTATGCGCCCTTGGATTGCTGTTGCAT 16 0.4 No Hit ATCTATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTG 16 0.4 No Hit AGCACAACATCCTTATGCATCCATTTCACATGTTAGGTGTAGCTGGTGTT 15 0.375 No Hit ATTTGGTCAAGAGGAAGAGACTTATAATATTGTGGCTGCTCATGGTTATT 15 0.375 No Hit CTATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAA 15 0.375 No Hit GTTCAACCTAAATGGATTCAATTTCAACCAATCTGTAGTTGATAGTCAAG 15 0.375 No Hit TATCTATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTT 15 0.375 No Hit GTCGTGAGTGGGAACTTAGTTTCCGTTTGGGTATGCGCCCTTGGATTGCT 14 0.35000000000000003 No Hit GGGAAACTACTGAAAATGAATCTGCTAATGAGGGTTACAAATTTGGTCAA 14 0.35000000000000003 No Hit GGAAGCTTCTCTGATGGTATGCCTTTAGGAATATCTGGTACTTTCAACTT 14 0.35000000000000003 No Hit GCTAATTGTTCTACACTTCTTACTTGGTGTAGCTTGTTATATGGGTCGTG 13 0.325 No Hit ATCGGATTGCACTTTTACCCAATTTGGGAAGCTGCATCCGTTGATGAATG 13 0.325 No Hit GTTAGGTGTAGCTGGTGTTTTCGGCGGTTCCCTATTCAGTGCTATGCATG 13 0.325 No Hit GTAGGAATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCT 12 0.3 No Hit GAGGAAGAGACTTATAATATTGTGGCTGCTCATGGTTATTTTGGCCGATT 12 0.3 No Hit GGCCGATTAATCTTCCAATATGCTAGTTTCAACAACTCTCGTTCTTTACA 12 0.3 No Hit GTTTGATCAGGGAAACTACTGAAAATGAATCTGCTAATGAGGGTTACAAA 12 0.3 No Hit GGAAGAGACTTATAATATTGTGGCTGCTCATGGTTATTTTGGCCGATTAA 11 0.27499999999999997 No Hit ATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAA 11 0.27499999999999997 No Hit GTTCGATCCTGGCTCAGGATGAACGCTGGCGGCATGCTTAACACATGCAA 11 0.27499999999999997 No Hit GGAATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCTAAA 11 0.27499999999999997 No Hit GCTGCATCCGTTGATGAATGGTTATACAATGGTGGTCCTTATGAGCTAAT 11 0.27499999999999997 No Hit GGTCGCTTCTGCAACTGGATAACTAGCACTGAAAATCGTCTTTACATCGG 10 0.25 No Hit GGAAACTACTGAAAATGAATCTGCTAATGAGGGTTACAAATTTGGTCAAG 10 0.25 No Hit GTTCCTTGGTAACCTCTAGTTTGATCAGGGAAACTACTGAAAATGAATCT 10 0.25 No Hit GTTGCATATTCAGCTCCTGTTGCAGCTGCGACTGCTGTTTTCTTGATTTA 10 0.25 No Hit ATTCAGTGCTATGCATGGTTCCTTGGTAACCTCTAGTTTGATCAGGGAAA 10 0.25 No Hit AGGGAAACTACTGAAAATGAATCTGCTAATGAGGGTTACAAATTTGGTCA 10 0.25 No Hit GGTAACCTCTAGTTTGATCAGGGAAACTACTGAAAATGAATCTGCTAATG 9 0.22499999999999998 No Hit GGTGTTTTGATGATCCCTACCTTATTGACCGCAACTTCTGTATTTATTAT 9 0.22499999999999998 No Hit GGCTTGCGGTGGATACCTAGGCACCCAGAGACGAGGAAGGGCGTAGCAAG 9 0.22499999999999998 No Hit GTTATTGTGAGAATTCTTAATTCAAGAGTTGTAAGGAGGGACTTATGTCA 9 0.22499999999999998 No Hit GACTTATAATATTGTGGCTGCTCATGGTTATTTTGGCCGATTAATCTTCC 8 0.2 No Hit AAACAATATTATCTCTGGTGCTATTATTCCTACTTCTGCGGCAATCGGAT 8 0.2 No Hit CTTAGTTTCCGTTTGGGTATGCGCCCTTGGATTGCTGTTGCATATTCAGC 8 0.2 No Hit ATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCA 8 0.2 No Hit CAGTGCTATGCATGGTTCCTTGGTAACCTCTAGTTTGATCAGGGAAACTA 8 0.2 No Hit ATTCAGCTCCTGTTGCAGCTGCGACTGCTGTTTTCTTGATTTACCCTATT 8 0.2 No Hit GGTCAAGGAAGCTTCTCTGATGGTATGCCTTTAGGAATATCTGGTACTTT 8 0.2 No Hit GAATGGTTATACAATGGTGGTCCTTATGAGCTAATTGTTCTACACTTCTT 8 0.2 No Hit CTGAAAATGAATCTGCTAATGAGGGTTACAAATTTGGTCAAGAGGAAGAG 8 0.2 No Hit GTATGCGCCCTTGGATTGCTGTTGCATATTCAGCTCCTGTTGCAGCTGCG 7 0.17500000000000002 No Hit GATTAATCTTCCAATATGCTAGTTTCAACAACTCTCGTTCTTTACACTTC 7 0.17500000000000002 No Hit GGCCTGTAGTAGGAATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCG 7 0.17500000000000002 No Hit AGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGATCT 7 0.17500000000000002 No Hit GTTACAAATTTGGTCAAGAGGAAGAGACTTATAATATTGTGGCTGCTCAT 7 0.17500000000000002 No Hit ATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCTAAATGG 7 0.17500000000000002 No Hit GCCTTTAGGAATATCTGGTACTTTCAACTTTATGATTGTATTCCAGGCAG 7 0.17500000000000002 No Hit GGGAACTTAGTTTCCGTTTGGGTATGCGCCCTTGGATTGCTGTTGCATAT 7 0.17500000000000002 No Hit GTACAAGCCTGTGGGGTCGCTTCTGCAACTGGATAACTAGCACTGAAAAT 7 0.17500000000000002 No Hit GGTACTTTCAACTTTATGATTGTATTCCAGGCAGAGCACAACATCCTTAT 7 0.17500000000000002 No Hit AGTAGATATTGATGGTATTCGCGAGCCTGTTTCTGGTTCTTTACTTTATG 7 0.17500000000000002 No Hit GTTCTTTACTTTATGGAAACAATATTATCTCTGGTGCTATTATTCCTACT 7 0.17500000000000002 No Hit CTTCTGCAACTGGATAACTAGCACTGAAAATCGTCTTTACATCGGATGGT 6 0.15 No Hit GTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCTAAATGGATTCA 6 0.15 No Hit GAATCTGCTAATGAGGGTTACAAATTTGGTCAAGAGGAAGAGACTTATAA 6 0.15 No Hit CTGGTACTTTCAACTTTATGATTGTATTCCAGGCAGAGCACAACATCCTT 6 0.15 No Hit ATTTCAACCAATCTGTAGTTGATAGTCAAGGTCGCGTTATTAATACTTGG 6 0.15 No Hit GTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGATCTT 6 0.15 No Hit GTACTATGGCGTTCAACCTAAATGGATTCAATTTCAACCAATCTGTAGTT 6 0.15 No Hit GCTAATGAGGGTTACAAATTTGGTCAAGAGGAAGAGACTTATAATATTGT 6 0.15 No Hit GCAATTTGAATAAACCAAGATCTTACCATGACTGCAATTTTAGAGAGACG 6 0.15 No Hit GTTATATGGGTCGTGAGTGGGAACTTAGTTTCCGTTTGGGTATGCGCCCT 6 0.15 No Hit GCATGGTTCCTTGGTAACCTCTAGTTTGATCAGGGAAACTACTGAAAATG 6 0.15 No Hit CTTACCAGGGCTTGACATGCCGCGAATCCTCTTGAAAGAGAGGGGTGCCC 6 0.15 No Hit GTGCTATGCATGGTTCCTTGGTAACCTCTAGTTTGATCAGGGAAACTACT 6 0.15 No Hit GTTGATAGTCAAGGTCGCGTTATTAATACTTGGGCTGATATCATCAACCG 6 0.15 No Hit GCCTGTAGTAGGAATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGT 6 0.15 No Hit GTTTCTGGTTCTTTACTTTATGGAAACAATATTATCTCTGGTGCTATTAT 6 0.15 No Hit CGGATGGTTCGGTGTTTTGATGATCCCTACCTTATTGACCGCAACTTCTG 6 0.15 No Hit GCTTTAGGTATTAGTACTATGGCGTTCAACCTAAATGGATTCAATTTCAA 6 0.15 No Hit GTTCTACACTTCTTACTTGGTGTAGCTTGTTATATGGGTCGTGAGTGGGA 6 0.15 No Hit CAACATCCTTATGCATCCATTTCACATGTTAGGTGTAGCTGGTGTTTTCG 6 0.15 No Hit GCTCATGGTTATTTTGGCCGATTAATCTTCCAATATGCTAGTTTCAACAA 5 0.125 No Hit GAAAGTACAAGCCTGTGGGGTCGCTTCTGCAACTGGATAACTAGCACTGA 5 0.125 No Hit TTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAA 5 0.125 No Hit CTTTTACCCAATTTGGGAAGCTGCATCCGTTGATGAATGGTTATACAATG 5 0.125 No Hit GTTTTCTTGATTTACCCTATTGGTCAAGGAAGCTTCTCTGATGGTATGCC 5 0.125 No Hit GCTTCTGCAACTGGATAACTAGCACTGAAAATCGTCTTTACATCGGATGG 5 0.125 No Hit GTCTTTACATCGGATGGTTCGGTGTTTTGATGATCCCTACCTTATTGACC 5 0.125 No Hit CAACTCTCGTTCTTTACACTTCTTCTTGGCTGCTTGGCCTGTAGTAGGAA 5 0.125 No Hit ATATCATCAACCGTGCTAATCTTGGTATGGAAGTAATGCACGAACGTAAT 5 0.125 No Hit GTTCGGTGTTTTGATGATCCCTACCTTATTGACCGCAACTTCTGTATTTA 5 0.125 No Hit TAGGAATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCTA 5 0.125 No Hit GTACTTTCAACTTTATGATTGTATTCCAGGCAGAGCACAACATCCTTATG 5 0.125 No Hit GGCTGCTTGGCCTGTAGTAGGAATCTGGTTCACTGCTTTAGGTATTAGTA 5 0.125 No Hit AATTTGGTCAAGAGGAAGAGACTTATAATATTGTGGCTGCTCATGGTTAT 5 0.125 No Hit GAGGGTTACAAATTTGGTCAAGAGGAAGAGACTTATAATATTGTGGCTGC 5 0.125 No Hit CGTTGATGAATGGTTATACAATGGTGGTCCTTATGAGCTAATTGTTCTAC 5 0.125 No Hit CGCAGCCCCTCCAGTAGATATTGATGGTATTCGCGAGCCTGTTTCTGGTT 5 0.125 No Hit CATGACTGCAATTTTAGAGAGACGCGAAAGTACAAGCCTGTGGGGTCGCT 5 0.125 No Hit GCTTGCGGTGGATACCTAGGCACCCAGAGACGAGGAAGGGCGTAGCAAGC 5 0.125 No Hit ATCTGGTACTTTCAACTTTATGATTGTATTCCAGGCAGAGCACAACATCC 5 0.125 No Hit ATTAGTACTATGGCGTTCAACCTAAATGGATTCAATTTCAACCAATCTGT 5 0.125 No Hit GTAGCTTGTTATATGGGTCGTGAGTGGGAACTTAGTTTCCGTTTGGGTAT 5 0.125 No Hit GGAAACAATATTATCTCTGGTGCTATTATTCCTACTTCTGCGGCAATCGG 5 0.125 No Hit GTTATTAATACTTGGGCTGATATCATCAACCGTGCTAATCTTGGTATGGA 5 0.125 No Hit AGCTGGTGTTTTCGGCGGTTCCCTATTCAGTGCTATGCATGGTTCCTTGG 5 0.125 No Hit ATGGCGTTCAACCTAAATGGATTCAATTTCAACCAATCTGTAGTTGATAG 5 0.125 No Hit GTTGATGAATGGTTATACAATGGTGGTCCTTATGAGCTAATTGTTCTACA 5 0.125 No Hit CACATGTTAGGTGTAGCTGGTGTTTTCGGCGGTTCCCTATTCAGTGCTAT 5 0.125 No Hit CCTTACCAGGGCTTGACATGCCGCGAATCCTCTTGAAAGAGAGGGGTGCC 5 0.125 No Hit TCTGTATTTATTATCGCCTTCATCGCAGCCCCTCCAGTAGATATTGATGG 5 0.125 No Hit CAGAGCACAACATCCTTATGCATCCATTTCACATGTTAGGTGTAGCTGGT 5 0.125 No Hit GAAACAATGACGGTATCTGAGGAATAAGCATCGGCTAACTCTGTGCCAGC 5 0.125 No Hit CTTTATGATTGTATTCCAGGCAGAGCACAACATCCTTATGCATCCATTTC 5 0.125 No Hit GCCGATTAATCTTCCAATATGCTAGTTTCAACAACTCTCGTTCTTTACAC 5 0.125 No Hit GTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGATCTTACCATGACTG 5 0.125 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0125 0.0 0.0 0.0 0.0 44-45 0.025 0.0 0.0 0.0 0.0 46-47 0.025 0.0 0.0 0.0 0.0 48-49 0.025 0.0 0.0 0.0 0.0 50-51 0.025 0.0 0.0 0.0 0.0 52-53 0.025 0.0 0.0 0.0 0.0 54-55 0.025 0.0 0.0 0.0 0.0 56-57 0.025 0.0 0.0 0.0 0.0 58-59 0.1125 0.0 0.0 0.0 0.0 60-61 0.125 0.0 0.0 0.0 0.0 62-63 0.125 0.0 0.0 0.0 0.0 64-65 0.1375 0.0 0.0 0.0 0.0 66-67 0.175 0.0 0.0 0.0 0.0 68-69 0.225 0.0 0.0 0.0 0.0 70-71 0.3 0.0 0.0 0.0 0.0 72-73 0.35 0.0 0.0 0.0 0.0 74-75 0.4 0.0 0.0 0.0 0.0 76-77 0.4375 0.0 0.0 0.0 0.0 78-79 0.4625 0.0 0.0 0.0 0.0 80-81 0.5874999999999999 0.0 0.0 0.0 0.0 82-83 0.6625000000000001 0.0 0.0 0.0 0.0 84-85 0.7625 0.0 0.0 0.0 0.0 86-87 0.8625 0.0 0.0 0.0 0.0 88-89 0.925 0.0 0.0 0.0 0.0 90-91 1.1375000000000002 0.0 0.0 0.0 0.0 92-93 1.325 0.0 0.0 0.0 0.0 94-95 1.5375 0.0 0.0 0.0 0.0 96-97 1.8 0.0 0.0 0.0 0.0 98-99 1.9749999999999999 0.0 0.0 0.0 0.0 100-101 2.2125 0.0 0.0 0.0 0.0 102-103 2.4875 0.0 0.0 0.0 0.0 104-105 2.7750000000000004 0.0 0.0 0.0 0.0 106-107 3.1125 0.0 0.0 0.0 0.0 108-109 3.5125 0.0 0.0 0.0 0.0 110-111 3.8125 0.0 0.0 0.0 0.0 112-113 4.15 0.0 0.0 0.0 0.0 114-115 4.575 0.0 0.0 0.0 0.0 116-117 4.85 0.0 0.0 0.0 0.0 118-119 5.137499999999999 0.0 0.0 0.0 0.0 120-121 5.45 0.0 0.0 0.0 0.0 122-123 5.7375 0.0 0.0 0.0 0.0 124-125 6.075 0.0 0.0 0.0 0.0 126-127 6.45 0.0 0.0 0.0 0.0 128-129 6.7375 0.0 0.0 0.0 0.0 130-131 7.1375 0.0 0.0 0.0 0.0 132-133 7.6375 0.0 0.0 0.0 0.0 134-135 7.9375 0.0 0.0 0.0 0.0 136-137 8.325 0.0 0.0 0.0 0.0 138-139 8.8 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GATGATC 35 0.0032537475 62.40868 9 TGATGAT 40 0.0055219056 54.607594 8 >>END_MODULE Read 1525691 spots for SRR6941547.sra Written 1525691 spots for SRR6941547.sra Read 1525691 spots for SRR6941547.sra Written 1525691 spots for SRR6941547.sra Read 1525691 spots for SRR6941547.sra Written 1525691 spots for SRR6941547.sra Read 1525691 spots for SRR6941547.sra Written 1525691 spots for SRR6941547.sra Read 1525691 spots for SRR6941547.sra Written 1525691 spots for SRR6941547.sra Read 1525691 spots for SRR6941547.sra Written 1525691 spots for SRR6941547.sra Read 1525691 spots for SRR6941547.sra Written 1525691 spots for SRR6941547.sra Read 1525691 spots for SRR6941547.sra Written 1525691 spots for SRR6941547.sra Read 1525691 spots for SRR6941547.sra Written 1525691 spots for SRR6941547.sra Read 1525691 spots for SRR6941547.sra Written 1525691 spots for SRR6941547.sra Read 1525693 spots for SRR6941547.sra Written 1525693 spots for SRR6941547.sra Read 1525691 spots for SRR6941547.sra Written 1525691 spots for SRR6941547.sra Read 1525691 spots for SRR6941547.sra Written 1525691 spots for SRR6941547.sra Read 1525691 spots for SRR6941547.sra Written 1525691 spots for SRR6941547.sra Read 1525691 spots for SRR6941547.sra Written 1525691 spots for SRR6941547.sra Read 1525691 spots for SRR6941547.sra Written 1525691 spots for SRR6941547.sra Read 1525691 spots for SRR6941547.sra Written 1525691 spots for SRR6941547.sra Read 1525691 spots for SRR6941547.sra Written 1525691 spots for SRR6941547.sra Read 1525691 spots for SRR6941547.sra Written 1525691 spots for SRR6941547.sra Read 1525691 spots for SRR6941547.sra Written 1525691 spots for SRR6941547.sra SRR ids: ['SRR6941547.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_tv4qkhrs SRR6941547.sra spots: 30513822 blocks: [[1, 1525691], [1525692, 3051382], [3051383, 4577073], [4577074, 6102764], [6102765, 7628455], [7628456, 9154146], [9154147, 10679837], [10679838, 12205528], [12205529, 13731219], [13731220, 15256910], [15256911, 16782601], [16782602, 18308292], [18308293, 19833983], [19833984, 21359674], [21359675, 22885365], [22885366, 24411056], [24411057, 25936747], [25936748, 27462438], [27462439, 28988129], [28988130, 30513822]] SRR6941547 file size 10318432 SRR6941547 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR6941547 SRR6941547_1.fastq SRR6941547_2.fastq Input file: SRR6941547_1.fastq Paired file: SRR6941547_2.fastq trimmed: SRR6941547-trimmed-pair1.fastq, SRR6941547-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Fri Dec 6 10:58:01 2024 >> started Fri Dec 6 10:58:39 2024 >> done (37.957s) 30513822 read pairs processed; of these: 16840 ( 0.06%) short read pairs filtered out after trimming by size control 75689 ( 0.25%) empty read pairs filtered out after trimming by size control 30421293 (99.70%) read pairs available; of these: 5887646 (19.35%) trimmed read pairs available after processing 24533647 (80.65%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 3 0.00% 19 4 0.00% 20 3 0.00% 21 7 0.00% 22 8 0.00% 23 8 0.00% 24 6 0.00% 25 10 0.00% 26 9 0.00% 27 23 0.00% 28 14 0.00% 29 28 0.00% 30 26 0.00% 31 27 0.00% 32 38 0.00% 33 39 0.00% 34 52 0.00% 35 60 0.00% 36 48 0.00% 37 72 0.00% 38 115 0.00% 39 112 0.00% 40 114 0.00% 41 159 0.00% 42 186 0.00% 43 212 0.00% 44 223 0.00% 45 229 0.00% 46 292 0.00% 47 336 0.00% 48 362 0.00% 49 461 0.00% 50 570 0.00% 51 643 0.00% 52 729 0.00% 53 758 0.00% 54 797 0.00% 55 1058 0.00% 56 1024 0.00% 57 1365 0.00% 58 1396 0.00% 59 1483 0.00% 60 1855 0.01% 61 2110 0.01% 62 2682 0.01% 63 3006 0.01% 64 3428 0.01% 65 3697 0.01% 66 3998 0.01% 67 4265 0.01% 68 4890 0.02% 69 5847 0.02% 70 6500 0.02% 71 7744 0.03% 72 8883 0.03% 73 9681 0.03% 74 9318 0.03% 75 10902 0.04% 76 10518 0.03% 77 12750 0.04% 78 12408 0.04% 79 13270 0.04% 80 14943 0.05% 81 16341 0.05% 82 18822 0.06% 83 18720 0.06% 84 20476 0.07% 85 24217 0.08% 86 24934 0.08% 87 25845 0.08% 88 29483 0.10% 89 28844 0.09% 90 33300 0.11% 91 33033 0.11% 92 38285 0.13% 93 37627 0.12% 94 39791 0.13% 95 42520 0.14% 96 40327 0.13% 97 39234 0.13% 98 38506 0.13% 99 39993 0.13% 100 41556 0.14% 101 44574 0.15% 102 49798 0.16% 103 48833 0.16% 104 50525 0.17% 105 53059 0.17% 106 51311 0.17% 107 52398 0.17% 108 53038 0.17% 109 56279 0.18% 110 58387 0.19% 111 63714 0.21% 112 65090 0.21% 113 62662 0.21% 114 68074 0.22% 115 61395 0.20% 116 63212 0.21% 117 59914 0.20% 118 60422 0.20% 119 59789 0.20% 120 61497 0.20% 121 63119 0.21% 122 75906 0.25% 123 75998 0.25% 124 78180 0.26% 125 78398 0.26% 126 73158 0.24% 127 73384 0.24% 128 72046 0.24% 129 80087 0.26% 130 75772 0.25% 131 83155 0.27% 132 81602 0.27% 133 72025 0.24% 134 83034 0.27% 135 78501 0.26% 136 84948 0.28% 137 80685 0.27% 138 91180 0.30% 139 90137 0.30% 140 84066 0.28% 141 102906 0.34% 142 88032 0.29% 143 95024 0.31% 144 92384 0.30% 145 106664 0.35% 146 115845 0.38% 147 122450 0.40% 148 152548 0.50% 149 213388 0.70% 150 1281395 4.21% 151 24533647 80.65% 30421293 reads passed initial QC criterion=sequence-density sequence-density=0.28 sequence-density-rank=1 fanout-score=1.99 fanout-score-rank=29 prefix-density=0.28 prefix-fanout=2.0 sequence=AATATACCCAATG criterion=fanout-score sequence-density=0.01 sequence-density-rank=35 fanout-score=47.60 fanout-score-rank=1 prefix-density=0.07 prefix-fanout=5.6 sequence=AAAAAAAAAGGGGGGTAAGGACCCGCTAAGCTCCTACTTTTTCATGTTTCCAATCCGATCCCTCCGATTACTATAGAGATGAACCCAATCCAGAATATGAACCATAAAAGAAAACACCTACTAAACCAATCACAAGAATACCAGTTACCGTACCTATCAGCCAAAGAGGAATTCTTCCAGTAGTATCGGCCATTTCCCCTACTTTCCTCCACATTTTATCAAGTGGTCATGCTAGAGACAAAAACAGTCATGGATAGTTATGTTATAAGGATGGTATCCTTCCAAATGGGATAAGAGAGTTCTTACTACTCTCTTCTTTTCTCTCAATTAAAGAAGTAATTGGAAAACAAAACAGCAAGTACAAAAATGAGTAATAAACCCCAGTATAGACTGGTACGATTCAATTCAACATTTTGTTCATTCGGGTTTGATTGTGTCATAGTTCTATAGTTGGAATTTAGTTTATCGTTGGATGAACTGCATTGCTGATATTGATCCCAAGAAAAAAACA criterion=sequence-density sequence-density=0.37 sequence-density-rank=1 fanout-score=1.97 fanout-score-rank=28 prefix-density=0.37 prefix-fanout=2.0 sequence=GCGTGAGGCTGG criterion=fanout-score sequence-density=0.02 sequence-density-rank=32 fanout-score=44.27 fanout-score-rank=1 prefix-density=0.84 prefix-fanout=1.0 sequence=TGGTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGTTAACGAACGAGACCTCAGCCTGCTAACTAGCTATGCGGAGCCATCCCTCCGCAGCTAGCTTCTTAGAGGGACTATCGCCGTTTAGGCGACGGAAGTTTGAGGCAATAACAGGTCTGTGATGCCCTTAGATGTTCTGGGCCGCACGCGCGCTACACTGATGTATTCAACGAGTATATAGCCTTGGCCGACAGGCCCGGGTAATCTTGGGAAATTTCATCGTGATGGGGATAGATCATTGCAATTGTTGGTCTTCAACGAGGAATGCCTAGTAAGCGCGAGTCATCAGCTCGCGTTGACTACGTCCCTGCCCTTTGTACACACC SRR6941547 testing PE reads STAR mapping to Ensembl genome Started job on | Dec 06 10:59:11 Started mapping on | Dec 06 10:59:11 Finished on | Dec 06 11:01:36 Mapping speed, Million of reads per hour | 755.29 Number of input reads | 30421293 Average input read length | 293 UNIQUE READS: Uniquely mapped reads number | 17810339 Uniquely mapped reads % | 58.55% Average mapped length | 294.43 Number of splices: Total | 3435664 Number of splices: Annotated (sjdb) | 3003581 Number of splices: GT/AG | 3164349 Number of splices: GC/AG | 39882 Number of splices: AT/AC | 16354 Number of splices: Non-canonical | 215079 Mismatch rate per base, % | 0.25% Deletion rate per base | 0.02% Deletion average length | 1.98 Insertion rate per base | 0.02% Insertion average length | 2.66 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 10488908 % of reads mapped to multiple loci | 34.48% Number of reads mapped to too many loci | 53142 % of reads mapped to too many loci | 0.17% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 6.05% % of reads unmapped: other | 0.75% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 2135326 2135326 2135326 N_multimapping 10488908 10488908 10488908 N_noFeature 7844651 17155379 8141980 N_ambiguous 667469 10047 318560 UnstrandedReadsAssigned:9298219 PositiveStrandReadsAssigned:644913 NegativeStrandReadsAssigned:9349799 Dataset is classified negative stranded MeadianReadLen=151 20thPercentileLength=151 echo kmer=147 SRR6941547 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,972 [index] number of k-mers: 66,720,672 [index] number of equivalence classes: 111,837 [quant] running in paired-end mode [quant] will process pair 1: SRR6941547-trimmed-pair1.fastq SRR6941547-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 30,421,293 reads, 16,564,645 reads pseudoaligned [quant] estimated average fragment length: 230.604 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,035 rounds 52973 SRR6941547.ke.tsv 35125 SRR6941547.se.tsv 88098 total ==> SRR6941547.ke.tsv <== target_id length eff_length est_counts tpm PNS24245 936 706.781 0 0 PNS24247 1044 814.396 8.33586 0.574818 PNS24249 1928 1698.4 9.13065 0.30191 PNS24246 1044 814.396 8.33586 0.574818 PNS24248 1044 814.396 8.33586 0.574818 PNS24244 1471 1241.4 36.8618 1.66756 PNS24243 293 104.566 0 0 KQK14069 1603 1373.4 530.362 21.6866 KQK14071 474 254.09 8.65547 1.91302 ==> SRR6941547.se.tsv <== BRADI_1g14170v3 707 BRADI_1g53295v3 40 BRADI_1g59795v3 16 BRADI_1g07683v3 0 BRADI_1g00485v3 0 BRADI_1g20270v3 98 BRADI_1g74790v3 24 BRADI_1g09890v3 0 BRADI_1g77505v3 46 BRADI_1g48960v3 0 SRR6941547 completed mapping pipeline successfully