Starting /dee2/code/volunteer_pipeline.sh SRR6941548 current disk space = 1551444885504 free memory = 1602296744 SRR6941548 SRAfilesize f92012744d6d907e659d4791722df8c5 SRR6941548.sra SRR6941548.sra file validated SRR6941548 is paired end SRR6941548 is conventional basespace SRR6941548 read1 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR6941548_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 34.621 35.0 35.0 35.0 35.0 35.0 2 34.629 35.0 35.0 35.0 35.0 35.0 3 34.633 35.0 35.0 35.0 35.0 35.0 4 34.6145 35.0 35.0 35.0 35.0 35.0 5 34.58075 35.0 35.0 35.0 35.0 35.0 6 39.44475 40.0 40.0 40.0 39.0 40.0 7 39.49975 40.0 40.0 40.0 39.0 40.0 8 39.263 40.0 40.0 40.0 39.0 40.0 9 39.4195 40.0 40.0 40.0 39.0 40.0 10-14 39.445350000000005 40.0 40.0 40.0 39.0 40.0 15-19 39.4628 40.0 40.0 40.0 39.0 40.0 20-24 39.368700000000004 40.0 40.0 40.0 38.8 40.0 25-29 39.4037 40.0 40.0 40.0 39.0 40.0 30-34 39.3749 40.0 40.0 40.0 39.0 40.0 35-39 39.345299999999995 40.0 40.0 40.0 39.0 40.0 40-44 39.3763 40.0 40.0 40.0 39.0 40.0 45-49 39.31615000000001 40.0 40.0 40.0 39.0 40.0 50-54 39.341049999999996 40.0 40.0 40.0 39.0 40.0 55-59 39.3146 40.0 40.0 40.0 39.0 40.0 60-64 39.292350000000006 40.0 40.0 40.0 39.0 40.0 65-69 39.29600000000001 40.0 40.0 40.0 39.0 40.0 70-74 39.2688 40.0 40.0 40.0 39.0 40.0 75-79 39.2586 40.0 40.0 40.0 38.8 40.0 80-84 39.18485 40.0 40.0 40.0 38.6 40.0 85-89 39.20989999999999 40.0 40.0 40.0 39.0 40.0 90-94 39.221500000000006 40.0 40.0 40.0 39.0 40.0 95-99 39.05275 40.0 39.8 40.0 38.2 40.0 100-104 38.33505 39.4 38.6 39.6 36.8 39.8 105-109 39.05375 40.0 39.6 40.0 38.0 40.0 110-114 39.18255 40.0 40.0 40.0 38.6 40.0 115-119 39.2076 40.0 40.0 40.0 39.0 40.0 120-124 39.165 40.0 40.0 40.0 38.4 40.0 125-129 39.045950000000005 40.0 39.8 40.0 38.0 40.0 130-134 39.045550000000006 40.0 39.6 40.0 37.8 40.0 135-139 38.87245 40.0 39.0 40.0 37.6 40.0 140-144 38.90220000000001 40.0 39.0 40.0 37.6 40.0 145-149 38.7695 40.0 39.0 40.0 37.2 40.0 150-151 37.349999999999994 39.5 37.5 40.0 33.5 40.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 19 1.0 20 0.0 21 0.0 22 0.0 23 0.0 24 2.0 25 1.0 26 2.0 27 8.0 28 9.0 29 17.0 30 19.0 31 22.0 32 31.0 33 33.0 34 50.0 35 78.0 36 95.0 37 156.0 38 242.0 39 3234.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 60.90338770388959 9.811794228356337 3.8393977415307403 25.44542032622334 2 24.25 10.325 29.675 35.75 3 24.675 19.525000000000002 25.4 30.4 4 27.3 25.025 21.349999999999998 26.325 5 25.124999999999996 33.225 23.075000000000003 18.575 6 19.900000000000002 37.675 20.075000000000003 22.35 7 14.124999999999998 32.550000000000004 38.375 14.95 8 17.875 27.450000000000003 31.3 23.375 9 18.075 23.75 34.599999999999994 23.575 10-14 20.165 33.645 25.295 20.895 15-19 20.68 31.545 24.4 23.375 20-24 18.316831683168317 31.48314831483148 26.61766176617662 23.582358235823584 25-29 21.145 31.225 26.68 20.95 30-34 21.654999999999998 33.205 24.435000000000002 20.705000000000002 35-39 21.715 30.17 26.41 21.705 40-44 19.28578573572072 30.844253275982798 25.767730319095726 24.10223066920076 45-49 20.345 29.37 28.925 21.36 50-54 21.845 30.959999999999997 24.959999999999997 22.235 55-59 20.06 30.005 26.105 23.830000000000002 60-64 19.18287743161474 30.164524678701802 26.814022103315498 23.838575786367954 65-69 19.685 31.89 25.56 22.865 70-74 20.13 29.9 24.6 25.369999999999997 75-79 20.765 29.235 27.150000000000002 22.85 80-84 21.575 29.959999999999997 25.435000000000002 23.03 85-89 21.33 29.78 26.31 22.58 90-94 18.195458637591276 31.544463339001698 26.29288786635991 23.967190157047114 95-99 20.465 31.61 23.810000000000002 24.115000000000002 100-104 20.36 32.45 23.905 23.285 105-109 20.285 31.674999999999997 24.97 23.07 110-114 21.62 29.17 24.315 24.895 115-119 20.345 32.550000000000004 23.315 23.79 120-124 21.98 30.525000000000002 22.48 25.014999999999997 125-129 20.815 30.705 23.835 24.645 130-134 21.38 31.35 23.455000000000002 23.815 135-139 21.555 31.080000000000002 23.94 23.425 140-144 22.34 30.005 25.0 22.655 145-149 20.419999999999998 31.185000000000002 23.830000000000002 24.565 150-151 21.3875 31.8125 22.112499999999997 24.6875 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.5 19 1.5 20 2.0 21 2.0 22 4.5 23 4.5 24 2.5 25 2.5 26 6.0 27 9.5 28 10.5 29 12.0 30 16.5 31 22.0 32 25.0 33 31.0 34 35.0 35 42.0 36 92.0 37 188.5 38 259.0 39 297.5 40 303.0 41 314.5 42 260.5 43 215.0 44 222.5 45 205.5 46 177.5 47 148.5 48 146.0 49 108.0 50 84.0 51 69.5 52 46.5 53 49.0 54 69.0 55 89.0 56 96.0 57 68.5 58 41.5 59 42.0 60 41.0 61 28.0 62 16.0 63 15.5 64 20.5 65 14.5 66 6.0 67 5.5 68 6.0 69 7.0 70 4.5 71 1.5 72 1.5 73 3.0 74 2.0 75 1.5 76 2.5 77 1.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.375 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.01 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.03 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.015 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.03 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.025000000000006 #Duplication Level Percentage of deduplicated Percentage of total 1 83.16527838667251 47.425 2 7.1021481806225335 8.1 3 3.3318719859710653 5.7 4 1.6220955721174923 3.6999999999999997 5 0.920648838228847 2.625 6 0.8329679964927663 2.85 7 0.7014467338886453 2.8000000000000003 8 0.17536168347216133 0.8 9 0.3068829460762823 1.575 >10 1.709776413853573 19.075 >50 0.131521262604121 5.35 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTGCTCTGCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTCCTA 77 1.925 No Hit GCGGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTACG 75 1.875 No Hit GGCCAAAATAACCATGAGCAGCCACAATATTATAAGTCTCTTCCTCTTGA 62 1.55 No Hit GCTCTGCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTCCTAAA 40 1.0 No Hit GGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTACGTT 36 0.8999999999999999 No Hit GCCGAAAACACCAGCTACACCTAACATGTGAAATGGATGCATAAGGATGT 33 0.8250000000000001 No Hit GGATGCATAAGGATGTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAA 33 0.8250000000000001 No Hit GCCAAAATAACCATGAGCAGCCACAATATTATAAGTCTCTTCCTCTTGAC 33 0.8250000000000001 No Hit GCTTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCA 32 0.8 No Hit GCGGAAAAACCTTATCCATTAATAGCGGGAACTTCAAGAGCAGCTAGATC 32 0.8 No Hit GTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTC 30 0.75 No Hit GTTGAAACTAGCATATTGGAAGATTAATCGGCCAAAATAACCATGAGCAG 27 0.675 No Hit GTGAGCATTACGTTCGTGCATTACTTCCATACCAAGATTAGCACGGTTGA 25 0.625 No Hit GCGACCTTGACTATCAACTACAGATTGGTTGAAATTGAATCCATTTAGGT 24 0.6 No Hit CTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCATTAAT 23 0.575 No Hit CCACAATATTATAAGTCTCTTCCTCTTGACCAAATTTGTAACCCTCATTA 23 0.575 No Hit CTCTGCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTCCTAAAG 22 0.5499999999999999 No Hit GCACGGTTGATGATATCAGCCCAAGTATTAATAACGCGACCTTGACTATC 20 0.5 No Hit GCATAAGGATGTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAAAGTA 20 0.5 No Hit GCGGTCAATAAGGTAGGGATCATCAAAACACCGAACCATCCGATGTAAAG 19 0.475 No Hit GGCGATAATAAATACAGAAGTTGCGGTCAATAAGGTAGGGATCATCAAAA 19 0.475 No Hit CCCAAGTATTAATAACGCGACCTTGACTATCAACTACAGATTGGTTGAAA 18 0.44999999999999996 No Hit GTTAGCGGAAAAACCTTATCCATTAATAGCGGGAACTTCAAGAGCAGCTA 18 0.44999999999999996 No Hit GCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTCCTAAAGGCAT 18 0.44999999999999996 No Hit GATGCATAAGGATGTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAAA 15 0.375 No Hit GCCAAGAAGAAGTGTAAAGAACGAGAGTTGTTGAAACTAGCATATTGGAA 15 0.375 No Hit GGCATGATGACTTGGCCTCATCCTCTCCTTCCTCCGGCTTAACACCGGCG 14 0.35000000000000003 No Hit CCCTCATTAGCAGATTCATTTTCAGTAGTTTCCCTGATCAAACTAGAGGT 14 0.35000000000000003 No Hit GGAGGGGCTGCGATGAAGGCGATAATAAATACAGAAGTTGCGGTCAATAA 13 0.325 No Hit GCATTACGTTCGTGCATTACTTCCATACCAAGATTAGCACGGTTGATGAT 13 0.325 No Hit GCTGCGATGAAGGCGATAATAAATACAGAAGTTGCGGTCAATAAGGTAGG 13 0.325 No Hit GGGGCTGCGATGAAGGCGATAATAAATACAGAAGTTGCGGTCAATAAGGT 13 0.325 No Hit GCTAGTTATCCAGTTGCAGAAGCGACCCCACAGGCTTGTACTTTCGCGTC 12 0.3 No Hit ACGCGACCTTGACTATCAACTACAGATTGGTTGAAATTGAATCCATTTAG 12 0.3 No Hit GCCCAAGTATTAATAACGCGACCTTGACTATCAACTACAGATTGGTTGAA 12 0.3 No Hit CGGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTACGT 11 0.27499999999999997 No Hit GTAGGGATCATCAAAACACCGAACCATCCGATGTAAAGACGATTTTCAGT 11 0.27499999999999997 No Hit GGTCAATAAGGTAGGGATCATCAAAACACCGAACCATCCGATGTAAAGAC 10 0.25 No Hit GTTGATGATATCAGCCCAAGTATTAATAACGCGACCTTGACTATCAACTA 10 0.25 No Hit CTCATAAGGACCACCATTGTATAACCATTCATCAACGGATGCAGCTTCCC 10 0.25 No Hit CTTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCAT 10 0.25 No Hit CCATGAGCAGCCACAATATTATAAGTCTCTTCCTCTTGACCAAATTTGTA 10 0.25 No Hit GTCTCTTCCTCTTGACCAAATTTGTAACCCTCATTAGCAGATTCATTTTC 9 0.22499999999999998 No Hit CCAGATTCCTACTACAGGCCAAGCAGCCAAGAAGAAGTGTAAAGAACGAG 9 0.22499999999999998 No Hit GTAAAGAACGAGAGTTGTTGAAACTAGCATATTGGAAGATTAATCGGCCA 9 0.22499999999999998 No Hit GCTAGATCTAGAGGGAAGTTGTGAGCATTACGTTCGTGCATTACTTCCAT 9 0.22499999999999998 No Hit CTCTTGACCAAATTTGTAACCCTCATTAGCAGATTCATTTTCAGTAGTTT 9 0.22499999999999998 No Hit GACCCATATAACAAGCTACACCAAGTAAGAAGTGTAGAACAATTAGCTCA 9 0.22499999999999998 No Hit GAGCATTACGTTCGTGCATTACTTCCATACCAAGATTAGCACGGTTGATG 9 0.22499999999999998 No Hit GGTTGATGATATCAGCCCAAGTATTAATAACGCGACCTTGACTATCAACT 8 0.2 No Hit GGGAAGTTGTGAGCATTACGTTCGTGCATTACTTCCATACCAAGATTAGC 8 0.2 No Hit CGGAAAAACCTTATCCATTAATAGCGGGAACTTCAAGAGCAGCTAGATCT 8 0.2 No Hit GTTCGCTGGAGTAGGCTGCGAGGCCCAAGCGGCCAAGGAGTGCAGCGGCC 8 0.2 No Hit GCGATAATAAATACAGAAGTTGCGGTCAATAAGGTAGGGATCATCAAAAC 7 0.17500000000000002 No Hit GCGATGAAGGCGATAATAAATACAGAAGTTGCGGTCAATAAGGTAGGGAT 7 0.17500000000000002 No Hit GGAAAAACCTTATCCATTAATAGCGGGAACTTCAAGAGCAGCTAGATCTA 7 0.17500000000000002 No Hit GGGGCATGATGACTTGGCCTCATCCTCTCCTTCCTCCGGCTTAACACCGG 7 0.17500000000000002 No Hit TTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCATT 7 0.17500000000000002 No Hit GATGATATCAGCCCAAGTATTAATAACGCGACCTTGACTATCAACTACAG 7 0.17500000000000002 No Hit CATATAACAAGCTACACCAAGTAAGAAGTGTAGAACAATTAGCTCATAAG 7 0.17500000000000002 No Hit TGAGCATTACGTTCGTGCATTACTTCCATACCAAGATTAGCACGGTTGAT 7 0.17500000000000002 No Hit GTTTCCATAAAGTAAAGAACCAGAAACAGGCTCGCGAATACCATCAATAT 7 0.17500000000000002 No Hit GGCCAAGCAGCCAAGAAGAAGTGTAAAGAACGAGAGTTGTTGAAACTAGC 7 0.17500000000000002 No Hit GTTGAAATTGAATCCATTTAGGTTGAACGCCATAGTACTAATACCTAAAG 7 0.17500000000000002 No Hit CTCATTAGCAGATTCATTTTCAGTAGTTTCCCTGATCAAACTAGAGGTTA 7 0.17500000000000002 No Hit AGTTGTTGAAACTAGCATATTGGAAGATTAATCGGCCAAAATAACCATGA 7 0.17500000000000002 No Hit GTGGGCATTACGTTCGTGCATTACTTCCATACCAAGATTAGCACGGTTGA 7 0.17500000000000002 No Hit CCTCATTAGCAGATTCATTTTCAGTAGTTTCCCTGATCAAACTAGAGGTT 7 0.17500000000000002 No Hit CGCGACCTTGACTATCAACTACAGATTGGTTGAAATTGAATCCATTTAGG 7 0.17500000000000002 No Hit GTGCAATCCGATTGCCGCAGAAGTAGGAATAATAGCACCAGAGATAATAT 6 0.15 No Hit GGGCGCATACCCAAACGGAAACTAAGTTCCCACTCACGACCCATATAACA 6 0.15 No Hit ACCAGCTACACCTAACATGTGAAATGGATGCATAAGGATGTTGTGCTCTG 6 0.15 No Hit ACCATGAGCAGCCACAATATTATAAGTCTCTTCCTCTTGACCAAATTTGT 6 0.15 No Hit CTGCGATGAAGGCGATAATAAATACAGAAGTTGCGGTCAATAAGGTAGGG 6 0.15 No Hit CTTGACTATCAACTACAGATTGGTTGAAATTGAATCCATTTAGGTTGAAC 6 0.15 No Hit CGAGAGTTGTTGAAACTAGCATATTGGAAGATTAATCGGCCAAAATAACC 6 0.15 No Hit GCTGAATATGCAACAGCAATCCAAGGGCGCATACCCAAACGGAAACTAAG 6 0.15 No Hit TCTGCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTCCTAAAGG 6 0.15 No Hit GTTGCGGTCAATAAGGTAGGGATCATCAAAACACCGAACCATCCGATGTA 6 0.15 No Hit AACGAGAGTTGTTGAAACTAGCATATTGGAAGATTAATCGGCCAAAATAA 6 0.15 No Hit GCATATTGGAAGATTAATCGGCCAAAATAACCATGAGCAGCCACAATATT 6 0.15 No Hit GGGATCATCAAAACACCGAACCATCCGATGTAAAGACGATTTTCAGTGCT 6 0.15 No Hit GGATGTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAAAGTACCAGAT 6 0.15 No Hit CATAAGGATGTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAAAGTAC 6 0.15 No Hit GTCCATGTACCAGTAGAAGATTCGGCAGCTACTGCAGCCCCTGCTTCTTC 6 0.15 No Hit GTAGGAATAATAGCACCAGAGATAATATTGTTTCCATAAAGTAAAGAACC 6 0.15 No Hit GGTAAATCAAGAAAACAGCAGTCGCAGCTGCAACAGGAGCTGAATATGCA 6 0.15 No Hit CCCCACTGCTGCCTCCCGTAGGAGTCTGGGCCGTGTCTCAGTCCCAGTGT 6 0.15 No Hit GTCATTGTTTCTTCTCCGAGAAAAGAAGTTGACGACCCGTAGGCCTTCCA 5 0.125 No Hit ATTACGTTCGTGCATTACTTCCATACCAAGATTAGCACGGTTGATGATAT 5 0.125 No Hit ACCAGATATTCCTAAAGGCATACCATCAGAGAAGCTTCCTTGACCAATAG 5 0.125 No Hit GAGGGAAGTTGTGAGCATTACGTTCGTGCATTACTTCCATACCAAGATTA 5 0.125 No Hit CCATATAACAAGCTACACCAAGTAAGAAGTGTAGAACAATTAGCTCATAA 5 0.125 No Hit CCACCATTGTATAACCATTCATCAACGGATGCAGCTTCCCAAATTGGGTA 5 0.125 No Hit GCCTTATCCATTAATAGCGGGAACTTCAAGAGCAGCTAGATCTAGAGGGA 5 0.125 No Hit GTCAATTAGAAGAATAAAGAAGAATTACTGCATTTCGTAACTTATTTTTT 5 0.125 No Hit GAGTTGTTGAAACTAGCATATTGGAAGATTAATCGGCCAAAATAACCATG 5 0.125 No Hit GCTACACCTAACATGTGAAATGGATGCATAAGGATGTTGTGCTCTGCCTG 5 0.125 No Hit AGTACTAATACCTAAAGCAGTGAACCAGATTCCTACTACAGGCCAAGCAG 5 0.125 No Hit CCCGTCAATTCCTTTGAGTTTCATTCTTGCGAACGTACTCCCCAGGCGGG 5 0.125 No Hit CATTAGCAGATTCATTTTCAGTAGTTTCCCTGATCAAACTAGAGGTTACC 5 0.125 No Hit ATCCAGTTGCAGAAGCGACCCCACAGGCTTGTACTTTCGCGTCTCTCTAA 5 0.125 No Hit AGCGGAAAAACCTTATCCATTAATAGCGGGAACTTCAAGAGCAGCTAGAT 5 0.125 No Hit GGTCGTTCGAGCTTTTCCTGGGAGTATGGCATCGGTTACATACTTCAGTG 5 0.125 No Hit GAAGGCGATAATAAATACAGAAGTTGCGGTCAATAAGGTAGGGATCATCA 5 0.125 No Hit CCTGTGTCGGTTTCGGGTACAGGTACCCTTTTGTTGAAGGTCGTTCGAGC 5 0.125 No Hit CCGGGAACGGATTCACCGCCGTATGGCTGACCGGCGATTACTAGCGATTC 5 0.125 No Hit GTCAATAAGGTAGGGATCATCAAAACACCGAACCATCCGATGTAAAGACG 5 0.125 No Hit GCTGCGGTTAAGAAATTACAACCTTCCAAATAGGAACTAGCCAATCCATG 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.025 0.0 0.0 0.0 0.0 58-59 0.025 0.0 0.0 0.0 0.0 60-61 0.025 0.0 0.0 0.0 0.0 62-63 0.025 0.0 0.0 0.0 0.0 64-65 0.025 0.0 0.0 0.0 0.0 66-67 0.05 0.0 0.0 0.0 0.0 68-69 0.05 0.0 0.0 0.0 0.0 70-71 0.1125 0.0 0.0 0.0 0.0 72-73 0.175 0.0 0.0 0.0 0.0 74-75 0.225 0.0 0.0 0.0 0.0 76-77 0.325 0.0 0.0 0.0 0.0 78-79 0.4375 0.0 0.0 0.0 0.0 80-81 0.475 0.0 0.0 0.0 0.0 82-83 0.575 0.0 0.0 0.0 0.0 84-85 0.675 0.0 0.0 0.0 0.0 86-87 0.8 0.0 0.0 0.0 0.0 88-89 0.9125000000000001 0.0 0.0 0.0 0.0 90-91 1.1375 0.0 0.0 0.0 0.0 92-93 1.4249999999999998 0.0 0.0 0.0 0.0 94-95 1.725 0.0 0.0 0.0 0.0 96-97 2.025 0.0 0.0 0.0 0.0 98-99 2.3125 0.0 0.0 0.0 0.0 100-101 2.5875 0.0 0.0 0.0 0.0 102-103 2.925 0.0 0.0 0.0 0.0 104-105 3.175 0.0 0.0 0.0 0.0 106-107 3.625 0.0 0.0 0.0 0.0 108-109 4.0125 0.0 0.0 0.0 0.0 110-111 4.425 0.0 0.0 0.0 0.0 112-113 4.887499999999999 0.0 0.0 0.0 0.0 114-115 5.199999999999999 0.0 0.0 0.0 0.0 116-117 5.8 0.0 0.0 0.0 0.0 118-119 6.3125 0.0 0.0 0.0 0.0 120-121 6.762499999999999 0.0 0.0 0.0 0.0 122-123 7.137499999999999 0.0 0.0 0.0 0.0 124-125 7.6 0.0 0.0 0.0 0.0 126-127 8.25 0.0 0.0 0.0 0.0 128-129 8.6875 0.0 0.0 0.0 0.0 130-131 9.1375 0.0 0.0 0.0 0.0 132-133 9.6875 0.0 0.0 0.0 0.0 134-135 10.2875 0.0 0.0 0.0 0.0 136-137 10.8375 0.0 0.0 0.0 0.0 138-139 11.425 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATAGCCC 10 0.006830828 145.0 9 CCCCCCC 20 0.00593511 29.0 140-144 >>END_MODULE SRR6941548 read2 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR6941548_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 34.22025 35.0 35.0 35.0 33.0 35.0 2 34.1335 35.0 35.0 35.0 33.0 35.0 3 33.923 35.0 35.0 35.0 32.0 35.0 4 34.25275 35.0 35.0 35.0 34.0 35.0 5 34.27375 35.0 35.0 35.0 33.0 35.0 6 38.83525 40.0 40.0 40.0 38.0 40.0 7 39.03275 40.0 40.0 40.0 39.0 40.0 8 39.0365 40.0 40.0 40.0 39.0 40.0 9 39.095 40.0 40.0 40.0 39.0 40.0 10-14 38.965 40.0 40.0 40.0 38.8 40.0 15-19 38.72685 40.0 40.0 40.0 37.8 40.0 20-24 39.0262 40.0 40.0 40.0 38.8 40.0 25-29 38.9704 40.0 40.0 40.0 38.8 40.0 30-34 38.9461 40.0 40.0 40.0 38.4 40.0 35-39 38.9157 40.0 40.0 40.0 38.6 40.0 40-44 39.01075 40.0 40.0 40.0 38.8 40.0 45-49 38.7616 40.0 39.8 40.0 37.6 40.0 50-54 38.88765 40.0 40.0 40.0 38.4 40.0 55-59 38.8072 40.0 40.0 40.0 38.0 40.0 60-64 38.9646 40.0 40.0 40.0 38.8 40.0 65-69 38.8486 40.0 39.8 40.0 38.2 40.0 70-74 38.6309 40.0 39.4 40.0 37.0 40.0 75-79 38.8007 40.0 39.8 40.0 37.8 40.0 80-84 38.82715 40.0 40.0 40.0 38.0 40.0 85-89 38.59505 40.0 39.0 40.0 37.0 40.0 90-94 38.5697 40.0 39.0 40.0 36.6 40.0 95-99 38.4437 40.0 39.0 40.0 36.2 40.0 100-104 37.275549999999996 38.8 37.8 39.4 33.6 39.6 105-109 38.11475 40.0 39.0 40.0 35.8 40.0 110-114 38.0876 40.0 39.0 40.0 35.6 40.0 115-119 37.880449999999996 40.0 39.0 40.0 34.4 40.0 120-124 37.945299999999996 40.0 39.0 40.0 35.0 40.0 125-129 37.6209 40.0 38.8 40.0 33.6 40.0 130-134 37.9906 40.0 39.0 40.0 35.6 40.0 135-139 37.69715000000001 40.0 39.0 40.0 34.8 40.0 140-144 37.4333 40.0 39.0 40.0 34.0 40.0 145-149 36.9491 40.0 39.0 40.0 32.0 40.0 150-151 34.926249999999996 38.5 35.0 39.5 25.0 40.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 15.0 3 2.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 1.0 11 2.0 12 1.0 13 2.0 14 2.0 15 0.0 16 0.0 17 2.0 18 6.0 19 3.0 20 3.0 21 10.0 22 7.0 23 10.0 24 8.0 25 19.0 26 14.0 27 17.0 28 16.0 29 10.0 30 27.0 31 33.0 32 40.0 33 55.0 34 70.0 35 78.0 36 111.0 37 180.0 38 358.0 39 2898.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 50.76845553036029 19.501133786848072 10.38044847568657 19.349962207105065 2 25.251256281407038 19.57286432160804 35.678391959798994 19.497487437185928 3 19.704186512910503 23.414389571321134 40.43619954875909 16.445224367009274 4 24.163100931286184 29.80115781525296 27.485527309338032 18.550213944122827 5 26.95106649937265 31.16687578419072 24.391468005018822 17.49058971141782 6 20.070334086912837 34.94096960562673 27.32981662898769 17.658879678472744 7 17.951292995229725 20.738137082601053 42.957569671102185 18.353000251067034 8 20.336008024072218 25.02507522567703 29.81444332998997 24.82447342026078 9 21.690070210631895 20.536609829488466 34.70411233701103 23.069207622868607 10-14 23.355246650273497 25.683745671701715 31.29924223415467 19.661765443870127 15-19 23.25733226195259 24.22659702691844 31.518682201687426 20.997388509441542 20-24 22.791351025936887 24.85325841569257 32.67445943912106 19.680931119249486 25-29 22.970396387355745 25.37380832915203 31.038635223281485 20.617160060210736 30-34 24.358074222668005 24.24272818455366 32.01103309929789 19.388164493480442 35-39 24.670178078755956 23.340857787810386 31.998996739403058 19.9899673940306 40-44 23.864947574374153 24.53719961872272 31.329955350423923 20.267897456479204 45-49 23.64302197250928 25.373733319955853 31.042440052172164 19.940804655362694 50-54 23.80713461441975 24.97115046911846 31.042095228538457 20.179619687923335 55-59 22.85886307761778 26.616828056795942 29.677386985098593 20.84692188048768 60-64 23.303405728043337 24.8081456588253 31.19827456487937 20.690174048251993 65-69 23.169936280166574 25.186894787015202 30.580502734433797 21.062666198384424 70-74 24.130838308332915 25.24456930717905 30.622585661967594 20.002006722520445 75-79 23.939986953685583 24.702694565708263 30.523357920618192 20.833960559987958 80-84 24.329204072420886 24.274035809218113 32.14303626059481 19.253723857766186 85-89 25.215646940822467 24.889669007021062 29.61885656970913 20.275827482447344 90-94 23.886436597110755 23.986757624398074 31.06440609951846 21.062399678972714 95-99 23.849162571457224 24.56122755992378 30.729114431852373 20.860495436766623 100-104 23.57846092023307 25.502310628892904 30.575647980711274 20.34358047016275 105-109 24.908148371835523 24.344456188031607 30.88227892697166 19.865116513161205 110-114 23.987523268098805 25.01383508577753 30.42209588972179 20.57654575640187 115-119 25.070380052282324 24.929619947717676 30.19806957570883 19.80193042429117 120-124 24.866498740554157 25.75818639798489 28.98740554156171 20.387909319899244 125-129 24.937217478653942 26.08739326971371 28.794575590155702 20.180813661476645 130-134 24.514733983707128 25.636125917731068 29.598712662174393 20.250427436387408 135-139 24.86404833836858 25.327291037260824 30.26183282980866 19.54682779456193 140-144 25.508969965732714 25.559363031646846 29.938520459584762 18.993146543035678 145-149 24.734537768607517 25.031452871018068 30.793618841527852 19.44039051884656 150-151 23.83706311289917 23.925069147598695 32.85139552426452 19.386472215237617 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 10.0 1 5.5 2 0.5 3 0.0 4 0.5 5 0.5 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.5 20 0.5 21 1.0 22 1.0 23 3.0 24 5.0 25 6.0 26 6.5 27 9.5 28 13.0 29 15.0 30 23.5 31 31.5 32 30.5 33 46.0 34 72.0 35 79.5 36 99.0 37 161.5 38 198.0 39 204.5 40 241.5 41 255.0 42 224.0 43 238.5 44 272.5 45 237.0 46 219.5 47 188.5 48 129.5 49 100.0 50 85.0 51 92.5 52 70.5 53 53.0 54 76.0 55 81.0 56 68.5 57 55.0 58 40.0 59 43.0 60 47.5 61 37.0 62 30.5 63 23.0 64 14.5 65 11.5 66 7.0 67 5.5 68 7.0 69 7.5 70 4.5 71 1.5 72 0.5 73 1.5 74 4.5 75 3.0 76 1.0 77 1.0 78 1.0 79 1.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.775 2 0.5 3 0.27499999999999997 4 0.675 5 0.375 6 0.475 7 0.42500000000000004 8 0.3 9 0.3 10-14 0.365 15-19 0.44 20-24 0.335 25-29 0.35000000000000003 30-34 0.3 35-39 0.325 40-44 0.335 45-49 0.33 50-54 0.345 55-59 0.345 60-64 0.315 65-69 0.345 70-74 0.335 75-79 0.35500000000000004 80-84 0.305 85-89 0.3 90-94 0.32 95-99 0.29 100-104 0.45999999999999996 105-109 0.655 110-114 0.615 115-119 0.54 120-124 0.75 125-129 0.44999999999999996 130-134 0.5700000000000001 135-139 0.7000000000000001 140-144 0.7799999999999999 145-149 0.645 150-151 0.575 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.650000000000006 #Duplication Level Percentage of deduplicated Percentage of total 1 81.99513381995133 50.55 2 7.542579075425791 9.3 3 3.7712895377128954 6.9750000000000005 4 1.7842660178426604 4.3999999999999995 5 1.1759935117599352 3.6249999999999996 6 1.094890510948905 4.05 7 0.48661800486618007 2.1 8 0.40551500405515 2.0 9 0.16220600162206003 0.8999999999999999 >10 1.5815085158150852 16.1 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GCACAACATCCTTATGCATCCATTTCACATGTTAGGTGTAGCTGGTGTTT 37 0.9249999999999999 No Hit GCTTGTTATATGGGTCGTGAGTGGGAACTTAGTTTCCGTTTGGGTATGCG 32 0.8 No Hit ATTATCTATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAAT 28 0.7000000000000001 No Hit GTGGGAACTTAGTTTCCGTTTGGGTATGCGCCCTTGGATTGCTGTTGCAT 27 0.675 No Hit GCTAATTGTTCTACACTTCTTACTTGGTGTAGCTTGTTATATGGGTCGTG 26 0.65 No Hit GCTAGTTTCAACAACTCTCGTTCTTTACACTTCTTCTTGGCTGCTTGGCC 25 0.625 No Hit GTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGATCTTACCATGAC 24 0.6 No Hit GTTATTTTGGCCGATTAATCTTCCAATATGCTAGTTTCAACAACTCTCGT 22 0.5499999999999999 No Hit GTTGCATATTCAGCTCCTGTTGCAGCTGCGACTGCTGTTTTCTTGATTTA 22 0.5499999999999999 No Hit CTATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAA 19 0.475 No Hit ATCTATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTG 19 0.475 No Hit GTTTCAACAACTCTCGTTCTTTACACTTCTTCTTGGCTGCTTGGCCTGTA 18 0.44999999999999996 No Hit GTTCGATCCTGGCTCAGGATGAACGCTGGCGGCATGCTTAACACATGCAA 17 0.42500000000000004 No Hit TATCTATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTT 17 0.42500000000000004 No Hit GGAAGCTTCTCTGATGGTATGCCTTTAGGAATATCTGGTACTTTCAACTT 16 0.4 No Hit GTTCAACCTAAATGGATTCAATTTCAACCAATCTGTAGTTGATAGTCAAG 16 0.4 No Hit AGCACAACATCCTTATGCATCCATTTCACATGTTAGGTGTAGCTGGTGTT 15 0.375 No Hit ATCGGATTGCACTTTTACCCAATTTGGGAAGCTGCATCCGTTGATGAATG 14 0.35000000000000003 No Hit ATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAA 14 0.35000000000000003 No Hit GTTTTGATGATCCCTACCTTATTGACCGCAACTTCTGTATTTATTATCGC 14 0.35000000000000003 No Hit GCATGGTTCCTTGGTAACCTCTAGTTTGATCAGGGAAACTACTGAAAATG 14 0.35000000000000003 No Hit GTTATACAATGGTGGTCCTTATGAGCTAATTGTTCTACACTTCTTACTTG 14 0.35000000000000003 No Hit GGAAGAGACTTATAATATTGTGGCTGCTCATGGTTATTTTGGCCGATTAA 13 0.325 No Hit GTCGTGAGTGGGAACTTAGTTTCCGTTTGGGTATGCGCCCTTGGATTGCT 13 0.325 No Hit GGGAAACTACTGAAAATGAATCTGCTAATGAGGGTTACAAATTTGGTCAA 13 0.325 No Hit ATTCAGTGCTATGCATGGTTCCTTGGTAACCTCTAGTTTGATCAGGGAAA 13 0.325 No Hit GCTGCATCCGTTGATGAATGGTTATACAATGGTGGTCCTTATGAGCTAAT 13 0.325 No Hit GTTGATAGTCAAGGTCGCGTTATTAATACTTGGGCTGATATCATCAACCG 12 0.3 No Hit AGGGAAACTACTGAAAATGAATCTGCTAATGAGGGTTACAAATTTGGTCA 12 0.3 No Hit GTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGATCTTACCATGACTG 12 0.3 No Hit CATGACTGCAATTTTAGAGAGACGCGAAAGTACAAGCCTGTGGGGTCGCT 11 0.27499999999999997 No Hit GCCTGTAGTAGGAATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGT 11 0.27499999999999997 No Hit GCTGCGACTGCTGTTTTCTTGATTTACCCTATTGGTCAAGGAAGCTTCTC 11 0.27499999999999997 No Hit CTTAGTTTCCGTTTGGGTATGCGCCCTTGGATTGCTGTTGCATATTCAGC 10 0.25 No Hit CTAGCACTGAAAATCGTCTTTACATCGGATGGTTCGGTGTTTTGATGATC 10 0.25 No Hit GGAATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCTAAA 10 0.25 No Hit GTAGGAATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCT 10 0.25 No Hit CCTTACCAGGGCTTGACATGCCGCGAATCCTCTTGAAAGAGAGGGGTGCC 10 0.25 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 10 0.25 No Hit GGAAACTACTGAAAATGAATCTGCTAATGAGGGTTACAAATTTGGTCAAG 9 0.22499999999999998 No Hit ATTTGGTCAAGAGGAAGAGACTTATAATATTGTGGCTGCTCATGGTTATT 9 0.22499999999999998 No Hit CAATGGTGGTCCTTATGAGCTAATTGTTCTACACTTCTTACTTGGTGTAG 9 0.22499999999999998 No Hit GTTGATGAATGGTTATACAATGGTGGTCCTTATGAGCTAATTGTTCTACA 9 0.22499999999999998 No Hit GGTCGCTTCTGCAACTGGATAACTAGCACTGAAAATCGTCTTTACATCGG 8 0.2 No Hit GCGCCCTTGGATTGCTGTTGCATATTCAGCTCCTGTTGCAGCTGCGACTG 8 0.2 No Hit CAACTCTCGTTCTTTACACTTCTTCTTGGCTGCTTGGCCTGTAGTAGGAA 8 0.2 No Hit CCTAAATGGATTCAATTTCAACCAATCTGTAGTTGATAGTCAAGGTCGCG 8 0.2 No Hit TATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAA 8 0.2 No Hit GTACTATGGCGTTCAACCTAAATGGATTCAATTTCAACCAATCTGTAGTT 8 0.2 No Hit GGTTATACAATGGTGGTCCTTATGAGCTAATTGTTCTACACTTCTTACTT 8 0.2 No Hit GTTAGGTGTAGCTGGTGTTTTCGGCGGTTCCCTATTCAGTGCTATGCATG 8 0.2 No Hit TCTATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGA 8 0.2 No Hit GAATGGTTATACAATGGTGGTCCTTATGAGCTAATTGTTCTACACTTCTT 8 0.2 No Hit GTATGCGCCCTTGGATTGCTGTTGCATATTCAGCTCCTGTTGCAGCTGCG 7 0.17500000000000002 No Hit CTTTTACCCAATTTGGGAAGCTGCATCCGTTGATGAATGGTTATACAATG 7 0.17500000000000002 No Hit TGTAGCTTGTTATATGGGTCGTGAGTGGGAACTTAGTTTCCGTTTGGGTA 7 0.17500000000000002 No Hit GTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGATCTT 7 0.17500000000000002 No Hit GTGCTATGCATGGTTCCTTGGTAACCTCTAGTTTGATCAGGGAAACTACT 7 0.17500000000000002 No Hit GGGAACTTAGTTTCCGTTTGGGTATGCGCCCTTGGATTGCTGTTGCATAT 7 0.17500000000000002 No Hit CAGGGAAACTACTGAAAATGAATCTGCTAATGAGGGTTACAAATTTGGTC 7 0.17500000000000002 No Hit AACATCCTTATGCATCCATTTCACATGTTAGGTGTAGCTGGTGTTTTCGG 7 0.17500000000000002 No Hit GTTCTACACTTCTTACTTGGTGTAGCTTGTTATATGGGTCGTGAGTGGGA 7 0.17500000000000002 No Hit GGTACTTTCAACTTTATGATTGTATTCCAGGCAGAGCACAACATCCTTAT 7 0.17500000000000002 No Hit GGGTCGTGAGTGGGAACTTAGTTTCCGTTTGGGTATGCGCCCTTGGATTG 7 0.17500000000000002 No Hit GTTCTTTACTTTATGGAAACAATATTATCTCTGGTGCTATTATTCCTACT 7 0.17500000000000002 No Hit GTTTGGGTATGCGCCCTTGGATTGCTGTTGCATATTCAGCTCCTGTTGCA 6 0.15 No Hit ATTATTCCTACTTCTGCGGCAATCGGATTGCACTTTTACCCAATTTGGGA 6 0.15 No Hit GTCGCTTCTGCAACTGGATAACTAGCACTGAAAATCGTCTTTACATCGGA 6 0.15 No Hit GACGCGAAAGTACAAGCCTGTGGGGTCGCTTCTGCAACTGGATAACTAGC 6 0.15 No Hit GCTTGGGAGTCCTTGCAATTTGAATAAACCAAGATCTTACCATGACTGCA 6 0.15 No Hit GAATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCTAAAT 6 0.15 No Hit GTTCGGTGTTTTGATGATCCCTACCTTATTGACCGCAACTTCTGTATTTA 6 0.15 No Hit GAGGGTTACAAATTTGGTCAAGAGGAAGAGACTTATAATATTGTGGCTGC 6 0.15 No Hit GGAATATCTGGTACTTTCAACTTTATGATTGTATTCCAGGCAGAGCACAA 6 0.15 No Hit GCTAATGAGGGTTACAAATTTGGTCAAGAGGAAGAGACTTATAATATTGT 6 0.15 No Hit GTTACAAATTTGGTCAAGAGGAAGAGACTTATAATATTGTGGCTGCTCAT 6 0.15 No Hit CTTGCAATTTGAATAAACCAAGATCTTACCATGACTGCAATTTTAGAGAG 6 0.15 No Hit ATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATA 6 0.15 No Hit ATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCTAAATGG 6 0.15 No Hit ATGCGCCCTTGGATTGCTGTTGCATATTCAGCTCCTGTTGCAGCTGCGAC 6 0.15 No Hit GTAGCTTGTTATATGGGTCGTGAGTGGGAACTTAGTTTCCGTTTGGGTAT 6 0.15 No Hit GGAAACAATATTATCTCTGGTGCTATTATTCCTACTTCTGCGGCAATCGG 6 0.15 No Hit TATACAATGGTGGTCCTTATGAGCTAATTGTTCTACACTTCTTACTTGGT 6 0.15 No Hit CAGTGCTATGCATGGTTCCTTGGTAACCTCTAGTTTGATCAGGGAAACTA 6 0.15 No Hit GGTCAAGGAAGCTTCTCTGATGGTATGCCTTTAGGAATATCTGGTACTTT 6 0.15 No Hit GAAGAGACTTATAATATTGTGGCTGCTCATGGTTATTTTGGCCGATTAAT 6 0.15 No Hit GTGTTTTCGGCGGTTCCCTATTCAGTGCTATGCATGGTTCCTTGGTAACC 6 0.15 No Hit GTTTGATCAGGGAAACTACTGAAAATGAATCTGCTAATGAGGGTTACAAA 6 0.15 No Hit CTCATGGTTATTTTGGCCGATTAATCTTCCAATATGCTAGTTTCAACAAC 6 0.15 No Hit GAGCACAACATCCTTATGCATCCATTTCACATGTTAGGTGTAGCTGGTGT 6 0.15 No Hit GGAAGCTGCATCCGTTGATGAATGGTTATACAATGGTGGTCCTTATGAGC 6 0.15 No Hit CAACATCCTTATGCATCCATTTCACATGTTAGGTGTAGCTGGTGTTTTCG 6 0.15 No Hit ATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGATCTTACCATG 5 0.125 No Hit GTTTTCGGCGGTTCCCTATTCAGTGCTATGCATGGTTCCTTGGTAACCTC 5 0.125 No Hit GCTTCTCTGATGGTATGCCTTTAGGAATATCTGGTACTTTCAACTTTATG 5 0.125 No Hit CTGCATCCGTTGATGAATGGTTATACAATGGTGGTCCTTATGAGCTAATT 5 0.125 No Hit AAATTTGGTCAAGAGGAAGAGACTTATAATATTGTGGCTGCTCATGGTTA 5 0.125 No Hit ACTTAGTTTCCGTTTGGGTATGCGCCCTTGGATTGCTGTTGCATATTCAG 5 0.125 No Hit GTTCCTTGGTAACCTCTAGTTTGATCAGGGAAACTACTGAAAATGAATCT 5 0.125 No Hit GAGACTTATAATATTGTGGCTGCTCATGGTTATTTTGGCCGATTAATCTT 5 0.125 No Hit GTGAGTGGGAACTTAGTTTCCGTTTGGGTATGCGCCCTTGGATTGCTGTT 5 0.125 No Hit GGCCTGTAGTAGGAATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCG 5 0.125 No Hit AATGAATCTGCTAATGAGGGTTACAAATTTGGTCAAGAGGAAGAGACTTA 5 0.125 No Hit ATTTATTATCGCCTTCATCGCAGCCCCTCCAGTAGATATTGATGGTATTC 5 0.125 No Hit GCTGTTTTCTTGATTTACCCTATTGGTCAAGGAAGCTTCTCTGATGGTAT 5 0.125 No Hit GTGGTCCTTATGAGCTAATTGTTCTACACTTCTTACTTGGTGTAGCTTGT 5 0.125 No Hit GGTCCTTATGAGCTAATTGTTCTACACTTCTTACTTGGTGTAGCTTGTTA 5 0.125 No Hit TTCCAATATGCTAGTTTCAACAACTCTCGTTCTTTACACTTCTTCTTGGC 5 0.125 No Hit GCCTTTAGGAATATCTGGTACTTTCAACTTTATGATTGTATTCCAGGCAG 5 0.125 No Hit GGATTGCACTTTTACCCAATTTGGGAAGCTGCATCCGTTGATGAATGGTT 5 0.125 No Hit ATTCAGCTCCTGTTGCAGCTGCGACTGCTGTTTTCTTGATTTACCCTATT 5 0.125 No Hit TGTAGCTGGTGTTTTCGGCGGTTCCCTATTCAGTGCTATGCATGGTTCCT 5 0.125 No Hit AGCTGGTGTTTTCGGCGGTTCCCTATTCAGTGCTATGCATGGTTCCTTGG 5 0.125 No Hit ATTGTATTCCAGGCAGAGCACAACATCCTTATGCATCCATTTCACATGTT 5 0.125 No Hit CGGATGGTTCGGTGTTTTGATGATCCCTACCTTATTGACCGCAACTTCTG 5 0.125 No Hit GTCAAGGTCGCGTTATTAATACTTGGGCTGATATCATCAACCGTGCTAAT 5 0.125 No Hit GGTTCTTTACTTTATGGAAACAATATTATCTCTGGTGCTATTATTCCTAC 5 0.125 No Hit GGATAACTAGCACTGAAAATCGTCTTTACATCGGATGGTTCGGTGTTTTG 5 0.125 No Hit GTACAAGCCTGTGGGGTCGCTTCTGCAACTGGATAACTAGCACTGAAAAT 5 0.125 No Hit AGAACCTTACCAGGGCTTGACATGCCGCGAATCCTCTTGAAAGAGAGGGG 5 0.125 No Hit AGTAGATATTGATGGTATTCGCGAGCCTGTTTCTGGTTCTTTACTTTATG 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.025 0.0 0.0 0.0 0.0 58-59 0.025 0.0 0.0 0.0 0.0 60-61 0.025 0.0 0.0 0.0 0.0 62-63 0.025 0.0 0.0 0.0 0.0 64-65 0.025 0.0 0.0 0.0 0.0 66-67 0.05 0.0 0.0 0.0 0.0 68-69 0.05 0.0 0.0 0.0 0.0 70-71 0.1125 0.0 0.0 0.0 0.0 72-73 0.175 0.0 0.0 0.0 0.0 74-75 0.25 0.0 0.0 0.0 0.0 76-77 0.35 0.0 0.0 0.0 0.0 78-79 0.4625 0.0 0.0 0.0 0.0 80-81 0.5 0.0 0.0 0.0 0.0 82-83 0.5874999999999999 0.0 0.0 0.0 0.0 84-85 0.675 0.0 0.0 0.0 0.0 86-87 0.8 0.0 0.0 0.0 0.0 88-89 0.9375 0.0 0.0 0.0 0.0 90-91 1.1 0.0 0.0 0.0 0.0 92-93 1.375 0.0 0.0 0.0 0.0 94-95 1.7000000000000002 0.0 0.0 0.0 0.0 96-97 2.0 0.0 0.0 0.0 0.0 98-99 2.3 0.0 0.0 0.0 0.0 100-101 2.6125 0.0 0.0 0.0 0.0 102-103 2.95 0.0 0.0 0.0 0.0 104-105 3.2 0.0 0.0 0.0 0.0 106-107 3.6500000000000004 0.0 0.0 0.0 0.0 108-109 4.0375 0.0 0.0 0.0 0.0 110-111 4.45 0.0 0.0 0.0 0.0 112-113 4.887499999999999 0.0 0.0 0.0 0.0 114-115 5.2125 0.0 0.0 0.0 0.0 116-117 5.825 0.0 0.0 0.0 0.0 118-119 6.3625 0.0 0.0 0.0 0.0 120-121 6.8125 0.0 0.0 0.0 0.0 122-123 7.175 0.0 0.0 0.0 0.0 124-125 7.6 0.0 0.0 0.0 0.0 126-127 8.275 0.0 0.0 0.0 0.0 128-129 8.7125 0.0 0.0 0.0 0.0 130-131 9.15 0.0 0.0 0.0 0.0 132-133 9.675 0.0 0.0 0.0 0.0 134-135 10.2625 0.0 0.0 0.0 0.0 136-137 10.774999999999999 0.0 0.0 0.0 0.0 138-139 11.3125 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content pass >>END_MODULE Read 1335677 spots for SRR6941548.sra Written 1335677 spots for SRR6941548.sra Read 1335677 spots for SRR6941548.sra Written 1335677 spots for SRR6941548.sra Read 1335677 spots for SRR6941548.sra Written 1335677 spots for SRR6941548.sra Read 1335677 spots for SRR6941548.sra Written 1335677 spots for SRR6941548.sra Read 1335677 spots for SRR6941548.sra Written 1335677 spots for SRR6941548.sra Read 1335677 spots for SRR6941548.sra Written 1335677 spots for SRR6941548.sra Read 1335677 spots for SRR6941548.sra Written 1335677 spots for SRR6941548.sra Read 1335677 spots for SRR6941548.sra Written 1335677 spots for SRR6941548.sra Read 1335687 spots for SRR6941548.sra Written 1335687 spots for SRR6941548.sra Read 1335677 spots for SRR6941548.sra Written 1335677 spots for SRR6941548.sra Read 1335677 spots for SRR6941548.sra Written 1335677 spots for SRR6941548.sra Read 1335677 spots for SRR6941548.sra Written 1335677 spots for SRR6941548.sra Read 1335677 spots for SRR6941548.sra Written 1335677 spots for SRR6941548.sra Read 1335677 spots for SRR6941548.sra Written 1335677 spots for SRR6941548.sra Read 1335677 spots for SRR6941548.sra Written 1335677 spots for SRR6941548.sra Read 1335677 spots for SRR6941548.sra Written 1335677 spots for SRR6941548.sra Read 1335677 spots for SRR6941548.sra Written 1335677 spots for SRR6941548.sra Read 1335677 spots for SRR6941548.sra Written 1335677 spots for SRR6941548.sra Read 1335677 spots for SRR6941548.sra Written 1335677 spots for SRR6941548.sra Read 1335677 spots for SRR6941548.sra Written 1335677 spots for SRR6941548.sra SRR ids: ['SRR6941548.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_90uhuwl0 SRR6941548.sra spots: 26713550 blocks: [[1, 1335677], [1335678, 2671354], [2671355, 4007031], [4007032, 5342708], [5342709, 6678385], [6678386, 8014062], [8014063, 9349739], [9349740, 10685416], [10685417, 12021093], [12021094, 13356770], [13356771, 14692447], [14692448, 16028124], [16028125, 17363801], [17363802, 18699478], [18699479, 20035155], [20035156, 21370832], [21370833, 22706509], [22706510, 24042186], [24042187, 25377863], [25377864, 26713550]] SRR6941548 file size 9030645 SRR6941548 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR6941548 SRR6941548_1.fastq SRR6941548_2.fastq Input file: SRR6941548_1.fastq Paired file: SRR6941548_2.fastq trimmed: SRR6941548-trimmed-pair1.fastq, SRR6941548-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Fri Dec 6 11:00:59 2024 >> started Fri Dec 6 11:01:30 2024 >> done (30.881s) 26713550 read pairs processed; of these: 13242 ( 0.05%) short read pairs filtered out after trimming by size control 32132 ( 0.12%) empty read pairs filtered out after trimming by size control 26668176 (99.83%) read pairs available; of these: 5574009 (20.90%) trimmed read pairs available after processing 21094167 (79.10%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 19 1 0.00% 20 1 0.00% 21 4 0.00% 22 8 0.00% 23 2 0.00% 24 3 0.00% 25 4 0.00% 26 7 0.00% 27 10 0.00% 28 6 0.00% 29 14 0.00% 30 21 0.00% 31 27 0.00% 32 29 0.00% 33 26 0.00% 34 44 0.00% 35 47 0.00% 36 43 0.00% 37 61 0.00% 38 83 0.00% 39 132 0.00% 40 127 0.00% 41 188 0.00% 42 197 0.00% 43 206 0.00% 44 263 0.00% 45 252 0.00% 46 323 0.00% 47 344 0.00% 48 468 0.00% 49 542 0.00% 50 643 0.00% 51 797 0.00% 52 917 0.00% 53 938 0.00% 54 1019 0.00% 55 1333 0.00% 56 1431 0.01% 57 1780 0.01% 58 1951 0.01% 59 2146 0.01% 60 2383 0.01% 61 2869 0.01% 62 3633 0.01% 63 3806 0.01% 64 4504 0.02% 65 4982 0.02% 66 5569 0.02% 67 5729 0.02% 68 6726 0.03% 69 7920 0.03% 70 8846 0.03% 71 10190 0.04% 72 11866 0.04% 73 12929 0.05% 74 12665 0.05% 75 15362 0.06% 76 13901 0.05% 77 16738 0.06% 78 15822 0.06% 79 16442 0.06% 80 18443 0.07% 81 19980 0.07% 82 21854 0.08% 83 21917 0.08% 84 23443 0.09% 85 27667 0.10% 86 27523 0.10% 87 28891 0.11% 88 32583 0.12% 89 31771 0.12% 90 37149 0.14% 91 34587 0.13% 92 40429 0.15% 93 39230 0.15% 94 41881 0.16% 95 44734 0.17% 96 40356 0.15% 97 39775 0.15% 98 38048 0.14% 99 39012 0.15% 100 40124 0.15% 101 42696 0.16% 102 47066 0.18% 103 47710 0.18% 104 47987 0.18% 105 50924 0.19% 106 48905 0.18% 107 49146 0.18% 108 50461 0.19% 109 53245 0.20% 110 55993 0.21% 111 62059 0.23% 112 62699 0.24% 113 58740 0.22% 114 68342 0.26% 115 54928 0.21% 116 57191 0.21% 117 57286 0.21% 118 56725 0.21% 119 55155 0.21% 120 55872 0.21% 121 55734 0.21% 122 70345 0.26% 123 70686 0.27% 124 76871 0.29% 125 75928 0.28% 126 67204 0.25% 127 66775 0.25% 128 68366 0.26% 129 74792 0.28% 130 73123 0.27% 131 81422 0.31% 132 76724 0.29% 133 66934 0.25% 134 76738 0.29% 135 75321 0.28% 136 80059 0.30% 137 75855 0.28% 138 85654 0.32% 139 84696 0.32% 140 77342 0.29% 141 97764 0.37% 142 80829 0.30% 143 88108 0.33% 144 84719 0.32% 145 93686 0.35% 146 105198 0.39% 147 112784 0.42% 148 138533 0.52% 149 189378 0.71% 150 1130594 4.24% 151 21094167 79.10% 26668176 reads passed initial QC criterion=sequence-density sequence-density=0.26 sequence-density-rank=1 fanout-score=1.95 fanout-score-rank=28 prefix-density=0.26 prefix-fanout=1.9 sequence=AATATACCCAATG criterion=fanout-score sequence-density=0.02 sequence-density-rank=28 fanout-score=34.75 fanout-score-rank=1 prefix-density=0.62 prefix-fanout=1.0 sequence=GCTGCTGGCACCAGACTTGCCCTCCAATGGATCCTCGTTAAGGGATTTAGATTGTACTCATTCCAATTACCAGACACTAATGTGCCCGGTATTGTTATTTATTGTCACTACCTCCCCGTGTCAGGATTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCTAATTCTCCGTCACCCGTCACCACCATGGTAGGCCCCTATCCTACCATCGAAAGTTGATAGGGCAGAAATTTGAATGATGCGTCGCCGGCACGAGGGCCGTGCGATCCGTCGAGTTATCATGAATCATCGGATCAGCGAGCAAAGCCCGCGTCAGCCTTTTATCTAATAAATGCGCCCCTCCCAGAAGTCGGGGTTTGTTGCACGTATTAGCTCTAGAATTACT criterion=sequence-density sequence-density=0.28 sequence-density-rank=1 fanout-score=1.98 fanout-score-rank=29 prefix-density=0.28 prefix-fanout=2.0 sequence=TTCACAGAGCAGCGACAACTGCCCGCTT criterion=fanout-score sequence-density=0.03 sequence-density-rank=27 fanout-score=34.11 fanout-score-rank=1 prefix-density=0.92 prefix-fanout=1.1 sequence=TGGTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGTTAACGAACGAGACCTCAGCCTGCTAACTAGCTATGCGGAGCCATCCCTCCGCAGCTAGCTTCTTAGAGGGACTATCGCCGTTTAGGCGACGGAAGTTTGAGGCAATAACAGGTCTGTGATGCCCTTAGATGTTCTGGGCCGCACGCGCGCTACACTGATGTATTCAACGAGTATATAGCCTTGGCCGACAGGCCCGGGTAATCTTGGGAAATTTCATCGTGATGGGGATAGATCATTGCAATTGTTGGTCTTCAACGAGGAATGCCTAGTAAGCGCGAGTCATCAGCTCGCGTTGACTACGTCCCTGCCCTTTGTACACACC SRR6941548 testing PE reads STAR mapping to Ensembl genome Started job on | Dec 06 11:02:06 Started mapping on | Dec 06 11:02:06 Finished on | Dec 06 11:04:03 Mapping speed, Million of reads per hour | 820.56 Number of input reads | 26668176 Average input read length | 291 UNIQUE READS: Uniquely mapped reads number | 15735160 Uniquely mapped reads % | 59.00% Average mapped length | 293.45 Number of splices: Total | 3231571 Number of splices: Annotated (sjdb) | 2817840 Number of splices: GT/AG | 2964986 Number of splices: GC/AG | 38992 Number of splices: AT/AC | 13542 Number of splices: Non-canonical | 214051 Mismatch rate per base, % | 0.23% Deletion rate per base | 0.01% Deletion average length | 2.01 Insertion rate per base | 0.01% Insertion average length | 2.61 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 8879002 % of reads mapped to multiple loci | 33.29% Number of reads mapped to too many loci | 67165 % of reads mapped to too many loci | 0.25% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 6.45% % of reads unmapped: other | 1.00% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 2063977 2063977 2063977 N_multimapping 8879002 8879002 8879002 N_noFeature 7487662 15242257 7731683 N_ambiguous 476253 8053 230737 UnstrandedReadsAssigned:7771245 PositiveStrandReadsAssigned:484850 NegativeStrandReadsAssigned:7772740 Dataset is classified negative stranded MeadianReadLen=151 20thPercentileLength=151 echo kmer=147 SRR6941548 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,972 [index] number of k-mers: 66,720,672 [index] number of equivalence classes: 111,837 [quant] running in paired-end mode [quant] will process pair 1: SRR6941548-trimmed-pair1.fastq SRR6941548-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 26,668,176 reads, 14,014,209 reads pseudoaligned [quant] estimated average fragment length: 217.592 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,011 rounds 52973 SRR6941548.ke.tsv 35125 SRR6941548.se.tsv 88098 total ==> SRR6941548.ke.tsv <== target_id length eff_length est_counts tpm PNS24245 936 719.832 0 0 PNS24247 1044 827.408 7.51153 0.5835 PNS24249 1928 1711.41 5.57168 0.20925 PNS24246 1044 827.408 7.51153 0.5835 PNS24248 1044 827.408 7.51153 0.5835 PNS24244 1471 1254.41 31.8937 1.63418 PNS24243 293 108.449 0 0 KQK14069 1603 1386.41 764.067 35.422 KQK14071 474 262.991 19.3367 4.72579 ==> SRR6941548.se.tsv <== BRADI_1g14170v3 1028 BRADI_1g53295v3 26 BRADI_1g59795v3 16 BRADI_1g07683v3 0 BRADI_1g00485v3 0 BRADI_1g20270v3 70 BRADI_1g74790v3 19 BRADI_1g09890v3 0 BRADI_1g77505v3 54 BRADI_1g48960v3 0 SRR6941548 completed mapping pipeline successfully