Starting /dee2/code/volunteer_pipeline.sh SRR6941549 current disk space = 1551578361856 free memory = 1602288928 SRR6941549 SRAfilesize 1508e64797df4d0ae1031ce66ccc1a51 SRR6941549.sra SRR6941549.sra file validated SRR6941549 is paired end SRR6941549 is conventional basespace SRR6941549 read1 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR6941549_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 34.61225 35.0 35.0 35.0 35.0 35.0 2 34.584 35.0 35.0 35.0 34.0 35.0 3 34.65375 35.0 35.0 35.0 35.0 35.0 4 34.56675 35.0 35.0 35.0 34.0 35.0 5 34.6065 35.0 35.0 35.0 34.0 35.0 6 39.419 40.0 40.0 40.0 39.0 40.0 7 39.4475 40.0 40.0 40.0 39.0 40.0 8 39.202 40.0 40.0 40.0 39.0 40.0 9 39.409 40.0 40.0 40.0 39.0 40.0 10-14 39.4585 40.0 40.0 40.0 39.0 40.0 15-19 39.46965 40.0 40.0 40.0 39.0 40.0 20-24 39.353899999999996 40.0 40.0 40.0 38.8 40.0 25-29 39.3971 40.0 40.0 40.0 39.0 40.0 30-34 39.410399999999996 40.0 40.0 40.0 39.0 40.0 35-39 39.378699999999995 40.0 40.0 40.0 39.0 40.0 40-44 39.342749999999995 40.0 40.0 40.0 39.0 40.0 45-49 39.329750000000004 40.0 40.0 40.0 39.0 40.0 50-54 39.351150000000004 40.0 40.0 40.0 39.0 40.0 55-59 39.3178 40.0 40.0 40.0 39.0 40.0 60-64 39.3402 40.0 40.0 40.0 39.0 40.0 65-69 39.318349999999995 40.0 40.0 40.0 39.0 40.0 70-74 39.3083 40.0 40.0 40.0 39.0 40.0 75-79 39.2813 40.0 40.0 40.0 38.8 40.0 80-84 39.2215 40.0 40.0 40.0 38.8 40.0 85-89 39.2255 40.0 40.0 40.0 39.0 40.0 90-94 39.289899999999996 40.0 40.0 40.0 39.0 40.0 95-99 39.09155 40.0 39.8 40.0 38.4 40.0 100-104 38.346450000000004 39.4 38.6 39.6 36.6 39.8 105-109 39.07575 40.0 39.6 40.0 38.2 40.0 110-114 39.25545 40.0 40.0 40.0 39.0 40.0 115-119 39.29675 40.0 40.0 40.0 39.0 40.0 120-124 39.20665 40.0 40.0 40.0 39.0 40.0 125-129 39.0561 40.0 39.8 40.0 38.2 40.0 130-134 39.02485 40.0 39.8 40.0 38.0 40.0 135-139 38.8366 40.0 39.2 40.0 37.4 40.0 140-144 38.90220000000001 40.0 39.2 40.0 37.6 40.0 145-149 38.781949999999995 40.0 39.0 40.0 37.2 40.0 150-151 37.411500000000004 39.5 37.5 40.0 34.0 40.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 14 1.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 1.0 24 1.0 25 1.0 26 1.0 27 7.0 28 8.0 29 11.0 30 20.0 31 31.0 32 20.0 33 48.0 34 52.0 35 64.0 36 81.0 37 144.0 38 285.0 39 3224.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 51.51667084482327 10.328403108548509 5.0639257959388315 33.0910002506894 2 22.675 10.25 31.2 35.875 3 21.25 16.825000000000003 25.724999999999998 36.199999999999996 4 25.224999999999998 24.775 21.75 28.249999999999996 5 25.0 32.0 23.400000000000002 19.6 6 19.85 34.375 22.275 23.5 7 15.1 29.925 38.75 16.225 8 17.424999999999997 24.575 32.875 25.124999999999996 9 18.224999999999998 23.35 35.125 23.3 10-14 20.385 30.445 26.035000000000004 23.135 15-19 20.979999999999997 28.860000000000003 25.25 24.91 20-24 19.32789918487773 29.464419662949442 26.418962844426662 24.788718307746162 25-29 22.335 27.705000000000002 27.51 22.45 30-34 21.81 29.415000000000003 25.82 22.955000000000002 35-39 21.17 27.62 26.924999999999997 24.285 40-44 19.824912456228112 28.884442221110557 26.3831915957979 24.907453726863434 45-49 21.245 27.065 28.544999999999998 23.145 50-54 21.72 27.73 25.915 24.635 55-59 20.39 27.615000000000002 26.900000000000002 25.095 60-64 20.275000000000002 27.87 26.68 25.174999999999997 65-69 20.235 29.285 26.229999999999997 24.25 70-74 20.51 27.935 25.735000000000003 25.82 75-79 20.445 28.050000000000004 27.525 23.98 80-84 21.275 28.78 26.419999999999998 23.525 85-89 21.965 28.465 25.380000000000003 24.19 90-94 20.77207720772077 28.552855285528555 26.03260326032603 24.64246424642464 95-99 21.125 29.830000000000002 23.89 25.155 100-104 21.32 30.165 24.425 24.09 105-109 20.294999999999998 28.994999999999997 25.590000000000003 25.119999999999997 110-114 21.47 27.644999999999996 25.505 25.380000000000003 115-119 20.599999999999998 30.814999999999998 23.325000000000003 25.259999999999998 120-124 21.6 29.765000000000004 22.68 25.955000000000002 125-129 21.085 28.544999999999998 24.705 25.665 130-134 21.925 28.62 23.630000000000003 25.825 135-139 22.189999999999998 28.615000000000002 24.735 24.46 140-144 23.145 28.315 24.77 23.77 145-149 20.695 29.459999999999997 24.335 25.509999999999998 150-151 20.9375 30.0 23.225 25.837500000000002 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 1.0 6 1.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.5 13 0.5 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.5 20 1.0 21 1.5 22 3.5 23 4.0 24 4.0 25 3.5 26 5.0 27 9.5 28 11.0 29 13.0 30 11.5 31 13.5 32 22.0 33 31.0 34 37.5 35 36.0 36 64.0 37 143.0 38 208.0 39 243.5 40 247.5 41 254.0 42 228.5 43 202.0 44 192.5 45 188.0 46 174.5 47 130.0 48 108.5 49 88.0 50 85.5 51 86.5 52 79.0 53 90.0 54 105.0 55 149.0 56 168.0 57 113.5 58 100.0 59 115.5 60 82.5 61 37.5 62 27.5 63 27.0 64 17.0 65 7.0 66 5.0 67 4.5 68 4.5 69 2.0 70 1.0 71 1.5 72 2.5 73 1.5 74 0.0 75 1.5 76 2.0 77 0.5 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.27499999999999997 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.015 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.05 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.01 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.9 #Duplication Level Percentage of deduplicated Percentage of total 1 80.34223706176962 48.125 2 10.225375626043405 12.25 3 3.672787979966611 6.6000000000000005 4 1.7529215358931551 4.2 5 1.0434056761268782 3.125 6 0.2921535893155259 1.05 7 0.5843071786310517 2.45 8 0.3756260434056761 1.7999999999999998 9 0.12520868113522537 0.675 >10 1.5025041736227045 17.075000000000003 >50 0.08347245409015025 2.65 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GCGGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTACG 54 1.35 No Hit GTGCTCTGCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTCCTA 52 1.3 No Hit GGCCAAAATAACCATGAGCAGCCACAATATTATAAGTCTCTTCCTCTTGA 48 1.2 No Hit GCCAAAATAACCATGAGCAGCCACAATATTATAAGTCTCTTCCTCTTGAC 40 1.0 No Hit GGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTACGTT 37 0.9249999999999999 No Hit CCCCACTGCTGCCTCCCGTAGGAGTCTGGGCCGTGTCTCAGTCCCAGTGT 34 0.8500000000000001 No Hit GGCATGATGACTTGGCCTCATCCTCTCCTTCCTCCGGCTTAACACCGGCG 31 0.775 No Hit GTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTC 28 0.7000000000000001 No Hit GCTCTGCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTCCTAAA 28 0.7000000000000001 No Hit GGATGCATAAGGATGTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAA 27 0.675 No Hit CTCTGCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTCCTAAAG 26 0.65 No Hit GCCGAAAACACCAGCTACACCTAACATGTGAAATGGATGCATAAGGATGT 23 0.575 No Hit GTGAGCATTACGTTCGTGCATTACTTCCATACCAAGATTAGCACGGTTGA 20 0.5 No Hit GCGGAAAAACCTTATCCATTAATAGCGGGAACTTCAAGAGCAGCTAGATC 19 0.475 No Hit GTTAGCGGAAAAACCTTATCCATTAATAGCGGGAACTTCAAGAGCAGCTA 18 0.44999999999999996 No Hit GCATAAGGATGTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAAAGTA 18 0.44999999999999996 No Hit GCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTCCTAAAGGCAT 18 0.44999999999999996 No Hit GTTGAAACTAGCATATTGGAAGATTAATCGGCCAAAATAACCATGAGCAG 18 0.44999999999999996 No Hit GCGACCTTGACTATCAACTACAGATTGGTTGAAATTGAATCCATTTAGGT 17 0.42500000000000004 No Hit CTTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCAT 17 0.42500000000000004 No Hit CCGGGAACGGATTCACCGCCGTATGGCTGACCGGCGATTACTAGCGATTC 16 0.4 No Hit CCATGAGCAGCCACAATATTATAAGTCTCTTCCTCTTGACCAAATTTGTA 15 0.375 No Hit GGCGATAATAAATACAGAAGTTGCGGTCAATAAGGTAGGGATCATCAAAA 14 0.35000000000000003 No Hit ACGCGACCTTGACTATCAACTACAGATTGGTTGAAATTGAATCCATTTAG 14 0.35000000000000003 No Hit CTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCATTAAT 13 0.325 No Hit CCCACTGCTGCCTCCCGTAGGAGTCTGGGCCGTGTCTCAGTCCCAGTGTG 13 0.325 No Hit GTCGGTTTCGGGTACAGGTACCCTTTTGTTGAAGGTCGTTCGAGCTTTTC 12 0.3 No Hit CCCTCATTAGCAGATTCATTTTCAGTAGTTTCCCTGATCAAACTAGAGGT 12 0.3 No Hit CCACAATATTATAAGTCTCTTCCTCTTGACCAAATTTGTAACCCTCATTA 12 0.3 No Hit CGGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTACGT 11 0.27499999999999997 No Hit GCTTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCA 11 0.27499999999999997 No Hit GATGCATAAGGATGTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAAA 11 0.27499999999999997 No Hit GCTTTCTTTTCCTCTGGCTACTAAGATGTTTCAGTTCGCCAGGTTGTCTC 11 0.27499999999999997 No Hit CCTGTGTCGGTTTCGGGTACAGGTACCCTTTTGTTGAAGGTCGTTCGAGC 11 0.27499999999999997 No Hit GCACGGTTGATGATATCAGCCCAAGTATTAATAACGCGACCTTGACTATC 10 0.25 No Hit CCCAAGTATTAATAACGCGACCTTGACTATCAACTACAGATTGGTTGAAA 10 0.25 No Hit CGGAAAAACCTTATCCATTAATAGCGGGAACTTCAAGAGCAGCTAGATCT 10 0.25 No Hit CGCGACCTTGACTATCAACTACAGATTGGTTGAAATTGAATCCATTTAGG 10 0.25 No Hit GGTCAATAAGGTAGGGATCATCAAAACACCGAACCATCCGATGTAAAGAC 9 0.22499999999999998 No Hit CTCCACGCGGCATTGCTCCGTCAGGCTTTCGCCCATTGCGGAAAATTCCC 9 0.22499999999999998 No Hit GGGGCTGCGATGAAGGCGATAATAAATACAGAAGTTGCGGTCAATAAGGT 9 0.22499999999999998 No Hit GCGGTCAATAAGGTAGGGATCATCAAAACACCGAACCATCCGATGTAAAG 8 0.2 No Hit GTCATTGTTTCTTCTCCGAGAAAAGAAGTTGACGACCCGTAGGCCTTCCA 8 0.2 No Hit GGTTGATGATATCAGCCCAAGTATTAATAACGCGACCTTGACTATCAACT 8 0.2 No Hit GTCTCTTCCTCTTGACCAAATTTGTAACCCTCATTAGCAGATTCATTTTC 8 0.2 No Hit GGGCATGATGACTTGGCCTCATCCTCTCCTTCCTCCGGCTTAACACCGGC 8 0.2 No Hit GCTAGTTATCCAGTTGCAGAAGCGACCCCACAGGCTTGTACTTTCGCGTC 8 0.2 No Hit GCTGCGATGAAGGCGATAATAAATACAGAAGTTGCGGTCAATAAGGTAGG 8 0.2 No Hit CCCGTCAATTCCTTTGAGTTTCATTCTTGCGAACGTACTCCCCAGGCGGG 8 0.2 No Hit CCTAGCTTTCGTCTCTCAGTGTCAGTGTCGGCCCAGCAGAGTGCTTTCGC 8 0.2 No Hit GGAGGGGCTGCGATGAAGGCGATAATAAATACAGAAGTTGCGGTCAATAA 7 0.17500000000000002 No Hit CTGCGATGAAGGCGATAATAAATACAGAAGTTGCGGTCAATAAGGTAGGG 7 0.17500000000000002 No Hit ATCAGAGAAGCTTCCTTGACCAATAGGGTAAATCAAGAAAACAGCAGTCG 7 0.17500000000000002 No Hit CAGTGAACCAGATTCCTACTACAGGCCAAGCAGCCAAGAAGAAGTGTAAA 7 0.17500000000000002 No Hit GGGAAGTTGTGAGCATTACGTTCGTGCATTACTTCCATACCAAGATTAGC 7 0.17500000000000002 No Hit CCTCACGGTACTACTTCGCTATCGGTCACCCAGGAGTATTTAGCCTTGCA 7 0.17500000000000002 No Hit CCAAGAAGAAGTGTAAAGAACGAGAGTTGTTGAAACTAGCATATTGGAAG 7 0.17500000000000002 No Hit GATGACTTGGCCTCATCCTCTCCTTCCTCCGGCTTAACACCGGCGGTCTG 7 0.17500000000000002 No Hit GTTGATGATATCAGCCCAAGTATTAATAACGCGACCTTGACTATCAACTA 7 0.17500000000000002 No Hit AGCGGAAAAACCTTATCCATTAATAGCGGGAACTTCAAGAGCAGCTAGAT 7 0.17500000000000002 No Hit CTCATTAGCAGATTCATTTTCAGTAGTTTCCCTGATCAAACTAGAGGTTA 7 0.17500000000000002 No Hit CGGTCAATAAGGTAGGGATCATCAAAACACCGAACCATCCGATGTAAAGA 7 0.17500000000000002 No Hit AGCAGCCACAATATTATAAGTCTCTTCCTCTTGACCAAATTTGTAACCCT 7 0.17500000000000002 No Hit CCTCATTAGCAGATTCATTTTCAGTAGTTTCCCTGATCAAACTAGAGGTT 7 0.17500000000000002 No Hit CGTCAATTCCTTTGAGTTTCATTCTTGCGAACGTACTCCCCAGGCGGGAT 6 0.15 No Hit GTCAATTCCTTTGAGTTTCATTCTTGCGAACGTACTCCCCAGGCGGGATA 6 0.15 No Hit ACATGCTCCACCGCTTGTGCGGGCCCCCGTCAATTCCTTTGAGTTTCATT 6 0.15 No Hit CATATAACAAGCTACACCAAGTAAGAAGTGTAGAACAATTAGCTCATAAG 6 0.15 No Hit GTACAAGGCCCGGGAACGGATTCACCGCCGTATGGCTGACCGGCGATTAC 6 0.15 No Hit GGTCGTTCGAGCTTTTCCTGGGAGTATGGCATCGGTTACATACTTCAGTG 6 0.15 No Hit GAGCATTACGTTCGTGCATTACTTCCATACCAAGATTAGCACGGTTGATG 6 0.15 No Hit CGCCATAGTACTAATACCTAAAGCAGTGAACCAGATTCCTACTACAGGCC 5 0.125 No Hit CCGCAGAAGTAGGAATAATAGCACCAGAGATAATATTGTTTCCATAAAGT 5 0.125 No Hit CTTGACTATCAACTACAGATTGGTTGAAATTGAATCCATTTAGGTTGAAC 5 0.125 No Hit GGAAAAACCTTATCCATTAATAGCGGGAACTTCAAGAGCAGCTAGATCTA 5 0.125 No Hit CCCTCTTCAAATAGATCTAATGGATAAGCTACATAACAGATCCATTGACT 5 0.125 No Hit ACGAGAGTTGTTGAAACTAGCATATTGGAAGATTAATCGGCCAAAATAAC 5 0.125 No Hit GCCCTCATTAGCAGATTCATTTTCAGTAGTTTCCCTGATCAAACTAGAGG 5 0.125 No Hit GTGTCGGTTTCGGGTACAGGTACCCTTTTGTTGAAGGTCGTTCGAGCTTT 5 0.125 No Hit GCTGAATATGCAACAGCAATCCAAGGGCGCATACCCAAACGGAAACTAAG 5 0.125 No Hit TTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCATT 5 0.125 No Hit CAAGTATTAATAACGCGACCTTGACTATCAACTACAGATTGGTTGAAATT 5 0.125 No Hit TGATGATATCAGCCCAAGTATTAATAACGCGACCTTGACTATCAACTACA 5 0.125 No Hit TCTGCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTCCTAAAGG 5 0.125 No Hit GGCTGACCGGCGATTACTAGCGATTCCTGCTTCATGCAGGCGAGTTGCAG 5 0.125 No Hit GCATTACGTTCGTGCATTACTTCCATACCAAGATTAGCACGGTTGATGAT 5 0.125 No Hit ACCTTATCCATTAATAGCGGGAACTTCAAGAGCAGCTAGATCTAGAGGGA 5 0.125 No Hit CTCATAAGGACCACCATTGTATAACCATTCATCAACGGATGCAGCTTCCC 5 0.125 No Hit GTCCCAGTGTGGCTGATCATCCTCTCGGACCAGCTACTGATCATCGCCTT 5 0.125 No Hit GTCCATGTACCAGTAGAAGATTCGGCAGCTACTGCAGCCCCTGCTTCTTC 5 0.125 No Hit GGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTACGTTC 5 0.125 No Hit GTCCAGTCAACTGCTGCGCCTCAACGCATTTCGGGGAGAACCAGCTAGCT 5 0.125 No Hit GACCCATATAACAAGCTACACCAAGTAAGAAGTGTAGAACAATTAGCTCA 5 0.125 No Hit GTCAATAAGGTAGGGATCATCAAAACACCGAACCATCCGATGTAAAGACG 5 0.125 No Hit CTCTGCCCCTACCGTACTCCAGCTTGGTAGTTTCCACCGCCTGTCCAGGG 5 0.125 No Hit ACCACATGCTCCACCGCTTGTGCGGGCCCCCGTCAATTCCTTTGAGTTTC 5 0.125 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0125 0.0 0.0 0.0 0.0 46-47 0.025 0.0 0.0 0.0 0.0 48-49 0.025 0.0 0.0 0.0 0.0 50-51 0.025 0.0 0.0 0.0 0.0 52-53 0.025 0.0 0.0 0.0 0.0 54-55 0.025 0.0 0.0 0.0 0.0 56-57 0.025 0.0 0.0 0.0 0.0 58-59 0.025 0.0 0.0 0.0 0.0 60-61 0.037500000000000006 0.0 0.0 0.0 0.0 62-63 0.05 0.0 0.0 0.0 0.0 64-65 0.0625 0.0 0.0 0.0 0.0 66-67 0.075 0.0 0.0 0.0 0.0 68-69 0.1125 0.0 0.0 0.0 0.0 70-71 0.125 0.0 0.0 0.0 0.0 72-73 0.1625 0.0 0.0 0.0 0.0 74-75 0.21250000000000002 0.0 0.0 0.0 0.0 76-77 0.275 0.0 0.0 0.0 0.0 78-79 0.35 0.0 0.0 0.0 0.0 80-81 0.3875 0.0 0.0 0.0 0.0 82-83 0.4375 0.0 0.0 0.0 0.0 84-85 0.575 0.0 0.0 0.0 0.0 86-87 0.825 0.0 0.0 0.0 0.0 88-89 0.975 0.0 0.0 0.0 0.0 90-91 1.1 0.0 0.0 0.0 0.0 92-93 1.25 0.0 0.0 0.0 0.0 94-95 1.375 0.0 0.0 0.0 0.0 96-97 1.4375 0.0 0.0 0.0 0.0 98-99 1.6125 0.0 0.0 0.0 0.0 100-101 1.725 0.0 0.0 0.0 0.0 102-103 1.875 0.0 0.0 0.0 0.0 104-105 2.0875 0.0 0.0 0.0 0.0 106-107 2.3499999999999996 0.0 0.0 0.0 0.0 108-109 2.6625 0.0 0.0 0.0 0.0 110-111 2.9125 0.0 0.0 0.0 0.0 112-113 3.25 0.0 0.0 0.0 0.0 114-115 3.55 0.0 0.0 0.0 0.0 116-117 3.7875 0.0 0.0 0.0 0.0 118-119 4.075 0.0 0.0 0.0 0.0 120-121 4.3875 0.0 0.0 0.0 0.0 122-123 4.7 0.0 0.0 0.0 0.0 124-125 5.012499999999999 0.0 0.0 0.0 0.0 126-127 5.4125 0.0 0.0 0.0 0.0 128-129 5.875 0.0 0.0 0.0 0.0 130-131 6.325 0.0 0.0 0.0 0.0 132-133 6.6875 0.0 0.0 0.0 0.0 134-135 6.925 0.0 0.0 0.0 0.0 136-137 7.362500000000001 0.0 0.0 0.0 0.0 138-139 7.675 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content pass >>END_MODULE SRR6941549 read2 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR6941549_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 34.09075 35.0 35.0 35.0 33.0 35.0 2 34.15075 35.0 35.0 35.0 33.0 35.0 3 33.87425 35.0 35.0 35.0 32.0 35.0 4 34.23125 35.0 35.0 35.0 33.0 35.0 5 34.2625 35.0 35.0 35.0 33.0 35.0 6 38.73775 40.0 40.0 40.0 37.0 40.0 7 39.01125 40.0 40.0 40.0 39.0 40.0 8 39.07325 40.0 40.0 40.0 39.0 40.0 9 39.0455 40.0 40.0 40.0 39.0 40.0 10-14 38.9137 40.0 40.0 40.0 38.0 40.0 15-19 38.5848 40.0 39.8 40.0 36.8 40.0 20-24 38.99455 40.0 40.0 40.0 38.4 40.0 25-29 38.8951 40.0 40.0 40.0 37.8 40.0 30-34 38.91455 40.0 40.0 40.0 37.6 40.0 35-39 38.95035 40.0 39.8 40.0 38.0 40.0 40-44 38.9978 40.0 40.0 40.0 38.4 40.0 45-49 38.728899999999996 40.0 39.6 40.0 37.4 40.0 50-54 38.86435 40.0 40.0 40.0 37.8 40.0 55-59 38.8112 40.0 39.6 40.0 37.4 40.0 60-64 38.91145 40.0 40.0 40.0 37.8 40.0 65-69 38.80495 40.0 39.8 40.0 37.4 40.0 70-74 38.53535 40.0 39.0 40.0 36.4 40.0 75-79 38.76965 40.0 39.2 40.0 37.4 40.0 80-84 38.816700000000004 40.0 39.4 40.0 37.4 40.0 85-89 38.4317 40.0 39.0 40.0 35.8 40.0 90-94 38.37955000000001 40.0 39.0 40.0 36.0 40.0 95-99 38.211400000000005 40.0 39.0 40.0 35.4 40.0 100-104 37.07254999999999 38.6 37.8 39.4 33.2 39.6 105-109 38.0351 40.0 39.0 40.0 35.2 40.0 110-114 37.9601 40.0 39.0 40.0 35.0 40.0 115-119 37.70845 40.0 39.0 40.0 33.4 40.0 120-124 37.73045 40.0 39.0 40.0 34.0 40.0 125-129 37.3891 40.0 38.8 40.0 32.8 40.0 130-134 37.79455 40.0 39.0 40.0 34.6 40.0 135-139 37.50005 40.0 39.0 40.0 33.6 40.0 140-144 37.2741 40.0 39.0 40.0 33.6 40.0 145-149 36.62115000000001 40.0 38.2 40.0 29.8 40.0 150-151 34.42425 38.5 35.0 39.5 23.0 40.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 8.0 3 0.0 4 0.0 5 0.0 6 0.0 7 2.0 8 1.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 2.0 15 0.0 16 0.0 17 2.0 18 4.0 19 3.0 20 5.0 21 3.0 22 11.0 23 15.0 24 8.0 25 18.0 26 17.0 27 25.0 28 23.0 29 33.0 30 41.0 31 40.0 32 47.0 33 64.0 34 69.0 35 88.0 36 116.0 37 174.0 38 391.0 39 2790.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 48.70178976556592 17.217040584824804 11.520040332745147 22.56112931686413 2 27.17227523857358 20.467101958814666 32.571572074334505 19.789050728277246 3 20.35043804755945 22.377972465581976 40.42553191489361 16.846057571964955 4 25.06914759869248 31.25471460900176 24.64168971586623 19.034448076439528 5 27.600902481825017 31.311105540235644 23.66507896715969 17.422913010779645 6 21.66164658634538 36.571285140562246 24.748995983935743 17.018072289156628 7 18.936543767243542 20.71733132681214 40.88286932530725 19.46325558063707 8 22.13319979969955 24.41161742613921 28.267401101652478 25.187781672508763 9 24.13620430645969 21.231847771657485 32.098147220831244 22.533800701051575 10-14 24.656779236396435 25.37328389618198 29.837659084076563 20.132277783345025 15-19 25.11786538268633 25.368642792657237 28.74410673086568 20.769385093790753 20-24 24.991235538638755 25.341813993088596 29.583813291931687 20.083137176340962 25-29 25.105210420841683 25.736472945891787 28.582164328657317 20.57615230460922 30-34 25.938908362543817 24.877315973960943 28.993490235353033 20.190285428142214 35-39 26.307091346153843 24.62439903846154 29.061498397435898 20.007011217948715 40-44 24.88229990984674 25.92407092056496 28.35820895522388 20.83542021436442 45-49 24.58934294871795 26.211939102564102 28.786057692307693 20.412660256410255 50-54 25.994190123209453 24.286286687368527 29.219673444856255 20.49984974456576 55-59 24.28263808903801 26.916720917422005 28.138614853022187 20.662026140517803 60-64 24.62817366918724 24.993740297461066 29.630927938304374 20.747158095047325 65-69 25.12394211027092 25.26916720917422 28.76959286894687 20.837297811607993 70-74 25.473209814722082 25.543314972458685 28.79819729594392 20.185277916875314 75-79 25.33553685897436 25.085136217948715 28.495592948717945 21.083733974358974 80-84 24.907361041562343 24.516775162744118 30.83625438157236 19.73960941412118 85-89 26.678684091933302 25.27665114415903 28.125782384457466 19.918882379450203 90-94 25.393168386256637 25.27797255334068 27.97756185515376 21.351297205248922 95-99 25.04380914234216 24.392930456115756 29.089270515195516 21.473989886346565 100-104 24.50321156162184 25.822962665596144 28.96928944199117 20.704536330790845 105-109 25.97004422999598 23.854041013268997 29.18677121029353 20.989143546441497 110-114 25.475113122171944 25.052790346907994 28.612368024132728 20.85972850678733 115-119 25.834965596906233 25.071568479734818 28.582190748832303 20.511275174526645 120-124 25.509639100015104 25.590174661498967 27.553228972668244 21.346957265817686 125-129 24.957350727546412 25.67987957852484 27.94279979929754 21.419969894631212 130-134 24.969855305466236 25.20096463022508 28.3108922829582 21.51828778135048 135-139 25.2377358490566 25.469182389937107 28.357232704402513 20.93584905660377 140-144 26.203666398066076 25.201450443190975 29.069298952457697 19.525584206285256 145-149 25.378311799306218 25.549243376401385 28.319340405208386 20.75310441908401 150-151 25.03454339907047 24.444165305866097 29.30536364778294 21.21592764728049 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 4.0 1 2.5 2 0.5 3 0.0 4 0.0 5 0.5 6 0.5 7 0.0 8 0.0 9 0.5 10 0.5 11 0.0 12 0.5 13 0.5 14 0.0 15 0.5 16 0.5 17 0.0 18 1.0 19 2.0 20 1.5 21 0.5 22 1.5 23 2.0 24 2.5 25 5.0 26 5.0 27 9.0 28 14.5 29 16.5 30 21.5 31 23.5 32 24.0 33 37.0 34 58.5 35 69.0 36 80.0 37 134.5 38 167.0 39 169.0 40 207.5 41 224.0 42 193.0 43 196.0 44 216.5 45 193.5 46 173.0 47 154.5 48 122.5 49 96.0 50 90.5 51 103.5 52 94.0 53 108.0 54 152.5 55 165.0 56 149.0 57 108.5 58 79.0 59 73.5 60 67.0 61 49.0 62 38.5 63 31.0 64 16.0 65 10.0 66 8.5 67 8.0 68 7.0 69 3.5 70 2.0 71 1.5 72 0.5 73 1.0 74 0.5 75 0.0 76 0.5 77 1.0 78 0.5 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.8250000000000001 2 0.44999999999999996 3 0.125 4 0.575 5 0.27499999999999997 6 0.4 7 0.325 8 0.15 9 0.15 10-14 0.21 15-19 0.31 20-24 0.165 25-29 0.2 30-34 0.15 35-39 0.16 40-44 0.16999999999999998 45-49 0.16 50-54 0.16999999999999998 55-59 0.155 60-64 0.155 65-69 0.155 70-74 0.15 75-79 0.16 80-84 0.15 85-89 0.145 90-94 0.16999999999999998 95-99 0.135 100-104 0.36 105-109 0.52 110-114 0.5499999999999999 115-119 0.445 120-124 0.6649999999999999 125-129 0.35000000000000003 130-134 0.48 135-139 0.625 140-144 0.72 145-149 0.545 150-151 0.4875 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 65.3 #Duplication Level Percentage of deduplicated Percentage of total 1 80.09188361408881 52.300000000000004 2 10.145482388973965 13.25 3 3.9816232771822357 7.8 4 1.9525267993874427 5.1 5 1.0719754977029097 3.5000000000000004 6 0.6125574272588056 2.4 7 0.6508422664624809 2.9749999999999996 8 0.3445635528330781 1.7999999999999998 9 0.22970903522205208 1.35 >10 0.9188361408882083 9.525 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTTCGATCCTGGCTCAGGATGAACGCTGGCGGCATGCTTAACACATGCAA 39 0.975 No Hit GCACAACATCCTTATGCATCCATTTCACATGTTAGGTGTAGCTGGTGTTT 32 0.8 No Hit TATCTATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTT 27 0.675 No Hit GCTTGTTATATGGGTCGTGAGTGGGAACTTAGTTTCCGTTTGGGTATGCG 22 0.5499999999999999 No Hit GCTAGTTTCAACAACTCTCGTTCTTTACACTTCTTCTTGGCTGCTTGGCC 21 0.525 No Hit GTGGGAACTTAGTTTCCGTTTGGGTATGCGCCCTTGGATTGCTGTTGCAT 21 0.525 No Hit ATTATCTATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAAT 18 0.44999999999999996 No Hit GCTAATTGTTCTACACTTCTTACTTGGTGTAGCTTGTTATATGGGTCGTG 16 0.4 No Hit GGAAGCTTCTCTGATGGTATGCCTTTAGGAATATCTGGTACTTTCAACTT 16 0.4 No Hit GCTGCATCCGTTGATGAATGGTTATACAATGGTGGTCCTTATGAGCTAAT 16 0.4 No Hit GTTATTTTGGCCGATTAATCTTCCAATATGCTAGTTTCAACAACTCTCGT 14 0.35000000000000003 No Hit GGAAGAGACTTATAATATTGTGGCTGCTCATGGTTATTTTGGCCGATTAA 12 0.3 No Hit GGGAAACTACTGAAAATGAATCTGCTAATGAGGGTTACAAATTTGGTCAA 12 0.3 No Hit GTTATACAATGGTGGTCCTTATGAGCTAATTGTTCTACACTTCTTACTTG 11 0.27499999999999997 No Hit GGCTTGCGGTGGATACCTAGGCACCCAGAGACGAGGAAGGGCGTAGCAAG 11 0.27499999999999997 No Hit GTTTCAACAACTCTCGTTCTTTACACTTCTTCTTGGCTGCTTGGCCTGTA 11 0.27499999999999997 No Hit GCTGCGACTGCTGTTTTCTTGATTTACCCTATTGGTCAAGGAAGCTTCTC 11 0.27499999999999997 No Hit CCTTACCAGGGCTTGACATGCCGCGAATCCTCTTGAAAGAGAGGGGTGCC 11 0.27499999999999997 No Hit AGCACAACATCCTTATGCATCCATTTCACATGTTAGGTGTAGCTGGTGTT 10 0.25 No Hit GCCTGACGGAGCAATGCCGCGTGGAGGTGGAAGGCCTACGGGTCGTCAAC 10 0.25 No Hit CTAGCACTGAAAATCGTCTTTACATCGGATGGTTCGGTGTTTTGATGATC 10 0.25 No Hit GTTGCATATTCAGCTCCTGTTGCAGCTGCGACTGCTGTTTTCTTGATTTA 10 0.25 No Hit GTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGATCTTACCATGAC 10 0.25 No Hit ATCTATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTG 10 0.25 No Hit CTTTTACCCAATTTGGGAAGCTGCATCCGTTGATGAATGGTTATACAATG 9 0.22499999999999998 No Hit CTGGTACTTTCAACTTTATGATTGTATTCCAGGCAGAGCACAACATCCTT 9 0.22499999999999998 No Hit ATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAA 9 0.22499999999999998 No Hit GTAGCTTGTTATATGGGTCGTGAGTGGGAACTTAGTTTCCGTTTGGGTAT 9 0.22499999999999998 No Hit GTTAGGTGTAGCTGGTGTTTTCGGCGGTTCCCTATTCAGTGCTATGCATG 9 0.22499999999999998 No Hit GAATGGTTATACAATGGTGGTCCTTATGAGCTAATTGTTCTACACTTCTT 9 0.22499999999999998 No Hit GGAAACTACTGAAAATGAATCTGCTAATGAGGGTTACAAATTTGGTCAAG 8 0.2 No Hit CCTGGCTCAGGATGAACGCTGGCGGCATGCTTAACACATGCAAGTCGAAC 8 0.2 No Hit GCCGCGAATCCTCTTGAAAGAGAGGGGTGCCCTCGGGAACGCGGACACAG 8 0.2 No Hit CTATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAA 8 0.2 No Hit GTTTTGATGATCCCTACCTTATTGACCGCAACTTCTGTATTTATTATCGC 8 0.2 No Hit TCTAGTTTGATCAGGGAAACTACTGAAAATGAATCTGCTAATGAGGGTTA 8 0.2 No Hit TATGCCTTTAGGAATATCTGGTACTTTCAACTTTATGATTGTATTCCAGG 8 0.2 No Hit TCTATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGA 8 0.2 No Hit GTTCAACCTAAATGGATTCAATTTCAACCAATCTGTAGTTGATAGTCAAG 8 0.2 No Hit GCTCATGGTTATTTTGGCCGATTAATCTTCCAATATGCTAGTTTCAACAA 7 0.17500000000000002 No Hit GCGCCCTTGGATTGCTGTTGCATATTCAGCTCCTGTTGCAGCTGCGACTG 7 0.17500000000000002 No Hit ATCGGATTGCACTTTTACCCAATTTGGGAAGCTGCATCCGTTGATGAATG 7 0.17500000000000002 No Hit GTCGTGAGTGGGAACTTAGTTTCCGTTTGGGTATGCGCCCTTGGATTGCT 7 0.17500000000000002 No Hit TGGGAACTTAGTTTCCGTTTGGGTATGCGCCCTTGGATTGCTGTTGCATA 7 0.17500000000000002 No Hit CTTGCGGTGGATACCTAGGCACCCAGAGACGAGGAAGGGCGTAGCAAGCG 7 0.17500000000000002 No Hit GCTAATGAGGGTTACAAATTTGGTCAAGAGGAAGAGACTTATAATATTGT 7 0.17500000000000002 No Hit AGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGATCT 7 0.17500000000000002 No Hit GTTACAAATTTGGTCAAGAGGAAGAGACTTATAATATTGTGGCTGCTCAT 7 0.17500000000000002 No Hit GTTGATAGTCAAGGTCGCGTTATTAATACTTGGGCTGATATCATCAACCG 7 0.17500000000000002 No Hit ATTCAGCTCCTGTTGCAGCTGCGACTGCTGTTTTCTTGATTTACCCTATT 7 0.17500000000000002 No Hit GTTGGGTTAAGTCTCGCAACGAGCGCAACCCTCGTGTTTAGTTGCCACTA 7 0.17500000000000002 No Hit GAGGAAGAGACTTATAATATTGTGGCTGCTCATGGTTATTTTGGCCGATT 7 0.17500000000000002 No Hit GTTGATGAATGGTTATACAATGGTGGTCCTTATGAGCTAATTGTTCTACA 7 0.17500000000000002 No Hit AGGGAAACTACTGAAAATGAATCTGCTAATGAGGGTTACAAATTTGGTCA 7 0.17500000000000002 No Hit CGGCAATCGGATTGCACTTTTACCCAATTTGGGAAGCTGCATCCGTTGAT 7 0.17500000000000002 No Hit GTTTGATCAGGGAAACTACTGAAAATGAATCTGCTAATGAGGGTTACAAA 7 0.17500000000000002 No Hit GTTTGGGTATGCGCCCTTGGATTGCTGTTGCATATTCAGCTCCTGTTGCA 6 0.15 No Hit CATGGTTATTTTGGCCGATTAATCTTCCAATATGCTAGTTTCAACAACTC 6 0.15 No Hit CTTATGAGCTAATTGTTCTACACTTCTTACTTGGTGTAGCTTGTTATATG 6 0.15 No Hit GTTCCTTGGTAACCTCTAGTTTGATCAGGGAAACTACTGAAAATGAATCT 6 0.15 No Hit CGTTGATGAATGGTTATACAATGGTGGTCCTTATGAGCTAATTGTTCTAC 6 0.15 No Hit ATTTGGTCAAGAGGAAGAGACTTATAATATTGTGGCTGCTCATGGTTATT 6 0.15 No Hit GTTATATGGGTCGTGAGTGGGAACTTAGTTTCCGTTTGGGTATGCGCCCT 6 0.15 No Hit CTTAGTTTCCGTTTGGGTATGCGCCCTTGGATTGCTGTTGCATATTCAGC 6 0.15 No Hit GGCGAAAGCCTGACGGAGCAATGCCGCGTGGAGGTGGAAGGCCTACGGGT 6 0.15 No Hit GGAATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCTAAA 6 0.15 No Hit GCTTGACATGCCGCGAATCCTCTTGAAAGAGAGGGGTGCCCTCGGGAACG 6 0.15 No Hit ATTGTATTCCAGGCAGAGCACAACATCCTTATGCATCCATTTCACATGTT 6 0.15 No Hit CTTCCAATATGCTAGTTTCAACAACTCTCGTTCTTTACACTTCTTCTTGG 6 0.15 No Hit AGAACCTTACCAGGGCTTGACATGCCGCGAATCCTCTTGAAAGAGAGGGG 6 0.15 No Hit GGTACTTTCAACTTTATGATTGTATTCCAGGCAGAGCACAACATCCTTAT 6 0.15 No Hit GTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGATCTTACCATGACTG 6 0.15 No Hit GGCTGTCGTCAGCTCGTGCCGTAAGGTGTTGGGTTAAGTCTCGCAACGAG 5 0.125 No Hit GTATGCGCCCTTGGATTGCTGTTGCATATTCAGCTCCTGTTGCAGCTGCG 5 0.125 No Hit GGTCGCTTCTGCAACTGGATAACTAGCACTGAAAATCGTCTTTACATCGG 5 0.125 No Hit ATTTGGGAAGCTGCATCCGTTGATGAATGGTTATACAATGGTGGTCCTTA 5 0.125 No Hit GGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCTAAATGGATTC 5 0.125 No Hit CCTACTTCTGCGGCAATCGGATTGCACTTTTACCCAATTTGGGAAGCTGC 5 0.125 No Hit GTCCTTATGAGCTAATTGTTCTACACTTCTTACTTGGTGTAGCTTGTTAT 5 0.125 No Hit GTTAAGTCTCGCAACGAGCGCAACCCTCGTGTTTAGTTGCCACTATGAGT 5 0.125 No Hit GGTGTTTTGATGATCCCTACCTTATTGACCGCAACTTCTGTATTTATTAT 5 0.125 No Hit GGAATATCTGGTACTTTCAACTTTATGATTGTATTCCAGGCAGAGCACAA 5 0.125 No Hit GGCCTGTAGTAGGAATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCG 5 0.125 No Hit GGAAGGCCTACGGGTCGTCAACTTCTTTTCTCGGAGAAGAAACAATGACG 5 0.125 No Hit GCTGTTTTCTTGATTTACCCTATTGGTCAAGGAAGCTTCTCTGATGGTAT 5 0.125 No Hit GGTTATACAATGGTGGTCCTTATGAGCTAATTGTTCTACACTTCTTACTT 5 0.125 No Hit GCATGGTTCCTTGGTAACCTCTAGTTTGATCAGGGAAACTACTGAAAATG 5 0.125 No Hit GGTCCTTATGAGCTAATTGTTCTACACTTCTTACTTGGTGTAGCTTGTTA 5 0.125 No Hit TATTGGTCAAGGAAGCTTCTCTGATGGTATGCCTTTAGGAATATCTGGTA 5 0.125 No Hit AGTCAAGGTCGCGTTATTAATACTTGGGCTGATATCATCAACCGTGCTAA 5 0.125 No Hit TATACAATGGTGGTCCTTATGAGCTAATTGTTCTACACTTCTTACTTGGT 5 0.125 No Hit GGTTCCTTGGTAACCTCTAGTTTGATCAGGGAAACTACTGAAAATGAATC 5 0.125 No Hit GGTCAAGGAAGCTTCTCTGATGGTATGCCTTTAGGAATATCTGGTACTTT 5 0.125 No Hit CTTATAATATTGTGGCTGCTCATGGTTATTTTGGCCGATTAATCTTCCAA 5 0.125 No Hit CGCAACTTCTGTATTTATTATCGCCTTCATCGCAGCCCCTCCAGTAGATA 5 0.125 No Hit GATTTACCCTATTGGTCAAGGAAGCTTCTCTGATGGTATGCCTTTAGGAA 5 0.125 No Hit GTTCTACACTTCTTACTTGGTGTAGCTTGTTATATGGGTCGTGAGTGGGA 5 0.125 No Hit GAAGCTTCTCTGATGGTATGCCTTTAGGAATATCTGGTACTTTCAACTTT 5 0.125 No Hit GGCTTTTCAAGTCCGCCGTCAAATCCCAGGGCTCAACCCTGGACAGGCGG 5 0.125 No Hit GCTAACTCCAAAAACCCGTCCTCAGTTCGGATTGCAGGCTGCAACTCGCC 5 0.125 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0125 0.0 0.0 0.0 0.0 46-47 0.025 0.0 0.0 0.0 0.0 48-49 0.025 0.0 0.0 0.0 0.0 50-51 0.025 0.0 0.0 0.0 0.0 52-53 0.025 0.0 0.0 0.0 0.0 54-55 0.025 0.0 0.0 0.0 0.0 56-57 0.025 0.0 0.0 0.0 0.0 58-59 0.025 0.0 0.0 0.0 0.0 60-61 0.037500000000000006 0.0 0.0 0.0 0.0 62-63 0.05 0.0 0.0 0.0 0.0 64-65 0.0625 0.0 0.0 0.0 0.0 66-67 0.075 0.0 0.0 0.0 0.0 68-69 0.1125 0.0 0.0 0.0 0.0 70-71 0.125 0.0 0.0 0.0 0.0 72-73 0.1625 0.0 0.0 0.0 0.0 74-75 0.21250000000000002 0.0 0.0 0.0 0.0 76-77 0.275 0.0 0.0 0.0 0.0 78-79 0.35 0.0 0.0 0.0 0.0 80-81 0.4125 0.0 0.0 0.0 0.0 82-83 0.475 0.0 0.0 0.0 0.0 84-85 0.625 0.0 0.0 0.0 0.0 86-87 0.875 0.0 0.0 0.0 0.0 88-89 1.025 0.0 0.0 0.0 0.0 90-91 1.1375 0.0 0.0 0.0 0.0 92-93 1.2875 0.0 0.0 0.0 0.0 94-95 1.4 0.0 0.0 0.0 0.0 96-97 1.4625 0.0 0.0 0.0 0.0 98-99 1.6375 0.0 0.0 0.0 0.0 100-101 1.75 0.0 0.0 0.0 0.0 102-103 1.9 0.0 0.0 0.0 0.0 104-105 2.0875 0.0 0.0 0.0 0.0 106-107 2.3375 0.0 0.0 0.0 0.0 108-109 2.625 0.0 0.0 0.0 0.0 110-111 2.9 0.0 0.0 0.0 0.0 112-113 3.2375 0.0 0.0 0.0 0.0 114-115 3.525 0.0 0.0 0.0 0.0 116-117 3.7625 0.0 0.0 0.0 0.0 118-119 4.025 0.0 0.0 0.0 0.0 120-121 4.3375 0.0 0.0 0.0 0.0 122-123 4.65 0.0 0.0 0.0 0.0 124-125 4.925 0.0 0.0 0.0 0.0 126-127 5.3125 0.0 0.0 0.0 0.0 128-129 5.7625 0.0 0.0 0.0 0.0 130-131 6.1875 0.0 0.0 0.0 0.0 132-133 6.55 0.0 0.0 0.0 0.0 134-135 6.7625 0.0 0.0 0.0 0.0 136-137 7.1875 0.0 0.0 0.0 0.0 138-139 7.5 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTTTTT 10 0.0065434803 147.05128 145 AAAACGG 10 0.0068008867 145.18987 4 >>END_MODULE Read 1753726 spots for SRR6941549.sra Written 1753726 spots for SRR6941549.sra Read 1753726 spots for SRR6941549.sra Written 1753726 spots for SRR6941549.sra Read 1753726 spots for SRR6941549.sra Written 1753726 spots for SRR6941549.sra Read 1753726 spots for SRR6941549.sra Written 1753726 spots for SRR6941549.sra Read 1753726 spots for SRR6941549.sra Written 1753726 spots for SRR6941549.sra Read 1753726 spots for SRR6941549.sra Written 1753726 spots for SRR6941549.sra Read 1753726 spots for SRR6941549.sra Written 1753726 spots for SRR6941549.sra Read 1753726 spots for SRR6941549.sra Written 1753726 spots for SRR6941549.sra Read 1753726 spots for SRR6941549.sra Written 1753726 spots for SRR6941549.sra Read 1753726 spots for SRR6941549.sra Read 1753726 spots for SRR6941549.sra Written 1753726 spots for SRR6941549.sra Written 1753726 spots for SRR6941549.sra Read 1753726 spots for SRR6941549.sra Written 1753726 spots for SRR6941549.sra Read 1753726 spots for SRR6941549.sra Written 1753726 spots for SRR6941549.sra Read 1753726 spots for SRR6941549.sra Written 1753726 spots for SRR6941549.sra Read 1753726 spots for SRR6941549.sra Written 1753726 spots for SRR6941549.sra Read 1753745 spots for SRR6941549.sra Written 1753745 spots for SRR6941549.sra Read 1753726 spots for SRR6941549.sra Written 1753726 spots for SRR6941549.sra Read 1753726 spots for SRR6941549.sra Written 1753726 spots for SRR6941549.sra Read 1753726 spots for SRR6941549.sra Written 1753726 spots for SRR6941549.sra Read 1753726 spots for SRR6941549.sra Written 1753726 spots for SRR6941549.sra SRR ids: ['SRR6941549.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd__d294woh SRR6941549.sra spots: 35074539 blocks: [[1, 1753726], [1753727, 3507452], [3507453, 5261178], [5261179, 7014904], [7014905, 8768630], [8768631, 10522356], [10522357, 12276082], [12276083, 14029808], [14029809, 15783534], [15783535, 17537260], [17537261, 19290986], [19290987, 21044712], [21044713, 22798438], [22798439, 24552164], [24552165, 26305890], [26305891, 28059616], [28059617, 29813342], [29813343, 31567068], [31567069, 33320794], [33320795, 35074539]] SRR6941549 file size 11863910 SRR6941549 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR6941549 SRR6941549_1.fastq SRR6941549_2.fastq Input file: SRR6941549_1.fastq Paired file: SRR6941549_2.fastq trimmed: SRR6941549-trimmed-pair1.fastq, SRR6941549-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Fri Dec 6 11:03:52 2024 >> started Fri Dec 6 11:04:33 2024 >> done (41.201s) 35074539 read pairs processed; of these: 15932 ( 0.05%) short read pairs filtered out after trimming by size control 40385 ( 0.12%) empty read pairs filtered out after trimming by size control 35018222 (99.84%) read pairs available; of these: 7166645 (20.47%) trimmed read pairs available after processing 27851577 (79.53%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 1 0.00% 19 6 0.00% 20 3 0.00% 21 2 0.00% 22 0 0.00% 23 2 0.00% 24 3 0.00% 25 3 0.00% 26 10 0.00% 27 5 0.00% 28 6 0.00% 29 23 0.00% 30 26 0.00% 31 30 0.00% 32 33 0.00% 33 39 0.00% 34 49 0.00% 35 41 0.00% 36 54 0.00% 37 48 0.00% 38 87 0.00% 39 106 0.00% 40 130 0.00% 41 137 0.00% 42 164 0.00% 43 184 0.00% 44 208 0.00% 45 208 0.00% 46 249 0.00% 47 280 0.00% 48 341 0.00% 49 400 0.00% 50 464 0.00% 51 573 0.00% 52 676 0.00% 53 699 0.00% 54 736 0.00% 55 931 0.00% 56 1001 0.00% 57 1153 0.00% 58 1360 0.00% 59 1558 0.00% 60 1718 0.00% 61 2231 0.01% 62 2585 0.01% 63 2770 0.01% 64 3144 0.01% 65 3504 0.01% 66 3731 0.01% 67 4048 0.01% 68 4809 0.01% 69 5845 0.02% 70 6176 0.02% 71 7463 0.02% 72 8729 0.02% 73 9560 0.03% 74 9261 0.03% 75 10745 0.03% 76 10873 0.03% 77 12988 0.04% 78 12249 0.03% 79 13535 0.04% 80 15320 0.04% 81 16551 0.05% 82 18108 0.05% 83 19555 0.06% 84 20648 0.06% 85 24284 0.07% 86 25321 0.07% 87 26384 0.08% 88 29780 0.09% 89 28973 0.08% 90 32659 0.09% 91 32333 0.09% 92 37367 0.11% 93 38399 0.11% 94 39407 0.11% 95 41829 0.12% 96 39132 0.11% 97 40149 0.11% 98 38201 0.11% 99 40160 0.11% 100 42034 0.12% 101 45554 0.13% 102 49915 0.14% 103 51185 0.15% 104 52626 0.15% 105 53549 0.15% 106 52421 0.15% 107 52771 0.15% 108 55272 0.16% 109 61384 0.18% 110 60723 0.17% 111 66611 0.19% 112 68584 0.20% 113 63078 0.18% 114 70967 0.20% 115 62146 0.18% 116 62794 0.18% 117 63532 0.18% 118 62423 0.18% 119 61863 0.18% 120 64985 0.19% 121 64286 0.18% 122 74370 0.21% 123 80702 0.23% 124 82548 0.24% 125 83420 0.24% 126 76379 0.22% 127 75966 0.22% 128 77179 0.22% 129 83312 0.24% 130 83579 0.24% 131 89212 0.25% 132 86246 0.25% 133 79639 0.23% 134 90317 0.26% 135 91400 0.26% 136 95589 0.27% 137 94160 0.27% 138 102294 0.29% 139 102042 0.29% 140 97438 0.28% 141 115326 0.33% 142 107651 0.31% 143 115837 0.33% 144 115243 0.33% 145 131130 0.37% 146 146277 0.42% 147 162126 0.46% 148 204631 0.58% 149 300861 0.86% 150 2080515 5.94% 151 27851577 79.53% 35018222 reads passed initial QC criterion=sequence-density sequence-density=0.35 sequence-density-rank=1 fanout-score=1.99 fanout-score-rank=29 prefix-density=0.34 prefix-fanout=2.0 sequence=CCAGCCTCACGC criterion=fanout-score sequence-density=0.02 sequence-density-rank=30 fanout-score=33.98 fanout-score-rank=1 prefix-density=0.30 prefix-fanout=2.0 sequence=AAAAACAGTAGAAGTAGAACAGGTATAAATAAGAAAATCTTAGTTAAGAGGGTTCATGTAAAGAACAGGTTCTAAATCACGATCGATTCCCTTTTCAAAACCTGCTGCAGCAGCTCGGGCTCTTCCTGCATGCCACAAATGGCCCACAAAAAAGAAGAATCCTAGAACAAAATGAGAAGTCGATAACCAACTTCTAGGAGAGACATAATTAACTGCATTGATCTCGGTAGCTACGCCACCCACGGAATTTAAAGAGCCTAAAGGAGCATGGGTCATATATTCCGCTGAACGTCGTTCTTGCCAAGGTTGTATGTCTTTTTTCAACCTACTCAAGTCCAAACCGTTGGGCCCCCTTAGAGGTTCTAACCATGGAGCACGGAGGTCCCAAAAACGCATAGTTTCCCCTCCAAAGATAACCTCTCCCGTTGGGGAACGCATTAGATATTTACCTAAACCTGTGGGTCCTTGAGCAGATCCCACATTAGCTCCAAGACGCTGGTCTCTAACTAGA criterion=sequence-density sequence-density=0.25 sequence-density-rank=1 fanout-score=2.30 fanout-score-rank=22 prefix-density=0.24 prefix-fanout=2.3 sequence=ATAACGGTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGACCCGCACGAAAGGCGTAACGATCTGGGCACTGTCTCGGAGAGAGGCTCGGTGAAATAGACATGTCTGTGAAGATGCGGACTACCTGCACCTGGACAGAAAGACCCTATGAAGCTTTACTGTTCCCTGGGATTGGCTTTGGGCCTTTCCTGCGCAGCTTAGGTGGAAGGCGAAGAAGGCCCCCTTCCGGGGGGGCCCGAGCCATCAGTGAGATACCACTCTGGAAGAGCTCGGATTCTAACCTTGTGTCAGACCCGCGGGCCAAGGGACAGTCTCAGGTAGACAGTTTCTATGGGGCGTAGGCCTCCCAAAAGGTAACGGAGGCGTGCAAAGGTTTCCTCGGGCCAGACGGACATTGGTCCTCGAGTGCAAAGGCAGAAGGGAGCTTGACTGCAAGACTCACCCGTCGAGCAGAGACGAAAGTCGGCCTTAGTGATCCGACGGTGCCGAGTGGAAGGGCCGTCGCTC criterion=fanout-score sequence-density=0.10 sequence-density-rank=23 fanout-score=24.71 fanout-score-rank=1 prefix-density=2.38 prefix-fanout=1.0 sequence=CAAGTCGAACGTTGTTTTCGGGGAGCTGGGCAGAAGGAAAAGAGGCTCCTAGCTAAAGTTGTCTCGCCCTGCTTCAAAACTACAGGGCGCGCGCTACGGCTTTGACCTAACGGCCTCCGTTTGCTGGAATCGGAATAGTTGAGAACAAAGTGGCGAACG SRR6941549 testing PE reads STAR mapping to Ensembl genome Started job on | Dec 06 11:05:17 Started mapping on | Dec 06 11:05:17 Finished on | Dec 06 11:08:04 Mapping speed, Million of reads per hour | 754.88 Number of input reads | 35018222 Average input read length | 294 UNIQUE READS: Uniquely mapped reads number | 19288290 Uniquely mapped reads % | 55.08% Average mapped length | 295.46 Number of splices: Total | 3426583 Number of splices: Annotated (sjdb) | 2934997 Number of splices: GT/AG | 3094618 Number of splices: GC/AG | 40470 Number of splices: AT/AC | 16514 Number of splices: Non-canonical | 274981 Mismatch rate per base, % | 0.22% Deletion rate per base | 0.01% Deletion average length | 1.96 Insertion rate per base | 0.01% Insertion average length | 2.62 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 12247215 % of reads mapped to multiple loci | 34.97% Number of reads mapped to too many loci | 221520 % of reads mapped to too many loci | 0.63% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 6.36% % of reads unmapped: other | 2.95% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 3493999 3493999 3493999 N_multimapping 12247215 12247215 12247215 N_noFeature 10429444 18722673 10693217 N_ambiguous 605345 9476 309534 UnstrandedReadsAssigned:8253501 PositiveStrandReadsAssigned:556141 NegativeStrandReadsAssigned:8285539 Dataset is classified negative stranded MeadianReadLen=151 20thPercentileLength=151 echo kmer=147 SRR6941549 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,972 [index] number of k-mers: 66,720,672 [index] number of equivalence classes: 111,837 [quant] running in paired-end mode [quant] will process pair 1: SRR6941549-trimmed-pair1.fastq SRR6941549-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 35,018,222 reads, 15,919,441 reads pseudoaligned [quant] estimated average fragment length: 227.823 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,115 rounds 52973 SRR6941549.ke.tsv 35125 SRR6941549.se.tsv 88098 total ==> SRR6941549.ke.tsv <== target_id length eff_length est_counts tpm PNS24245 936 709.462 13.6929 0.818173 PNS24247 1044 817.177 3.29569 0.170966 PNS24249 1928 1701.18 6.81793 0.169896 PNS24246 1044 817.177 3.29569 0.170966 PNS24248 1044 817.177 3.29569 0.170966 PNS24244 1471 1244.18 29.6021 1.0086 PNS24243 293 101.098 0 0 KQK14069 1603 1376.18 619.872 19.0944 KQK14071 474 253.263 15.9046 2.66214 ==> SRR6941549.se.tsv <== BRADI_1g14170v3 801 BRADI_1g53295v3 47 BRADI_1g59795v3 9 BRADI_1g07683v3 0 BRADI_1g00485v3 0 BRADI_1g20270v3 56 BRADI_1g74790v3 17 BRADI_1g09890v3 0 BRADI_1g77505v3 43 BRADI_1g48960v3 0 SRR6941549 completed mapping pipeline successfully