Starting /dee2/code/volunteer_pipeline.sh SRR6941550 current disk space = 1551591104512 free memory = 1600009436 SRR6941550 SRAfilesize f0650c203f44c734b541cb273b101343 SRR6941550.sra SRR6941550.sra file validated SRR6941550 is paired end SRR6941550 is conventional basespace SRR6941550 read1 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR6941550_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 34.6265 35.0 35.0 35.0 35.0 35.0 2 34.65325 35.0 35.0 35.0 35.0 35.0 3 34.6145 35.0 35.0 35.0 35.0 35.0 4 34.64 35.0 35.0 35.0 35.0 35.0 5 34.579 35.0 35.0 35.0 34.0 35.0 6 39.44225 40.0 40.0 40.0 39.0 40.0 7 39.441 40.0 40.0 40.0 39.0 40.0 8 39.26975 40.0 40.0 40.0 39.0 40.0 9 39.4215 40.0 40.0 40.0 39.0 40.0 10-14 39.448299999999996 40.0 40.0 40.0 39.0 40.0 15-19 39.46685 40.0 40.0 40.0 39.0 40.0 20-24 39.3331 40.0 40.0 40.0 39.0 40.0 25-29 39.411750000000005 40.0 40.0 40.0 39.0 40.0 30-34 39.412549999999996 40.0 40.0 40.0 39.0 40.0 35-39 39.3692 40.0 40.0 40.0 39.0 40.0 40-44 39.3891 40.0 40.0 40.0 39.0 40.0 45-49 39.344899999999996 40.0 40.0 40.0 39.0 40.0 50-54 39.38935 40.0 40.0 40.0 39.0 40.0 55-59 39.33325000000001 40.0 40.0 40.0 39.0 40.0 60-64 39.32064999999999 40.0 40.0 40.0 39.0 40.0 65-69 39.27265 40.0 40.0 40.0 39.0 40.0 70-74 39.2811 40.0 40.0 40.0 39.0 40.0 75-79 39.2572 40.0 40.0 40.0 38.8 40.0 80-84 39.257999999999996 40.0 40.0 40.0 38.8 40.0 85-89 39.25005 40.0 40.0 40.0 39.0 40.0 90-94 39.2725 40.0 40.0 40.0 39.0 40.0 95-99 39.12155 40.0 39.8 40.0 38.6 40.0 100-104 38.31515 39.4 38.6 39.6 36.6 39.8 105-109 39.06035000000001 40.0 39.6 40.0 38.2 40.0 110-114 39.202749999999995 40.0 40.0 40.0 39.0 40.0 115-119 39.2632 40.0 40.0 40.0 39.0 40.0 120-124 39.19590000000001 40.0 40.0 40.0 39.0 40.0 125-129 39.0732 40.0 40.0 40.0 38.2 40.0 130-134 38.998650000000005 40.0 39.8 40.0 37.8 40.0 135-139 38.9101 40.0 39.0 40.0 37.4 40.0 140-144 38.936350000000004 40.0 39.0 40.0 37.6 40.0 145-149 38.7915 40.0 39.0 40.0 37.4 40.0 150-151 37.47925 39.5 37.5 40.0 34.0 40.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 22 1.0 23 1.0 24 1.0 25 0.0 26 2.0 27 6.0 28 10.0 29 13.0 30 13.0 31 21.0 32 32.0 33 43.0 34 53.0 35 73.0 36 85.0 37 144.0 38 287.0 39 3215.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 50.66366140746305 11.16954670673679 5.334335086401202 32.83245679939895 2 22.575 13.625000000000002 32.4 31.4 3 21.075 19.025 26.674999999999997 33.225 4 24.65 27.474999999999998 21.8 26.075 5 24.45 31.874999999999996 23.7 19.975 6 19.0 36.925000000000004 22.7 21.375 7 14.75 28.65 40.925 15.675 8 16.175 26.974999999999998 32.550000000000004 24.3 9 17.349999999999998 23.5 35.975 23.175 10-14 18.88 33.25 25.145 22.725 15-19 20.205000000000002 30.195 26.35 23.25 20-24 18.9928489273391 30.389558433765064 27.444116617492625 23.17347602140321 25-29 21.709999999999997 30.525000000000002 27.025 20.74 30-34 22.31 31.64 25.0 21.05 35-39 21.224999999999998 30.294999999999998 26.77 21.709999999999997 40-44 19.576851898164357 30.170559695893562 26.804381533536738 23.448206872405343 45-49 19.46 29.665000000000003 28.46 22.415 50-54 21.105 29.705 26.255 22.935 55-59 20.73 29.62 26.155 23.494999999999997 60-64 19.155 30.12 27.495000000000005 23.23 65-69 19.59 30.675 26.275 23.46 70-74 20.064999999999998 30.375000000000004 24.685000000000002 24.875 75-79 20.919999999999998 29.29 26.834999999999997 22.955000000000002 80-84 21.7 30.005 25.19 23.105 85-89 21.855 29.17 26.25 22.725 90-94 18.890944547227363 30.876543827191362 26.851342567128356 23.381169058452922 95-99 20.145 30.55 24.759999999999998 24.545 100-104 19.505 31.345 25.869999999999997 23.28 105-109 19.78 30.755 26.625 22.84 110-114 21.255 29.360000000000003 25.965 23.419999999999998 115-119 20.07 30.425 24.465 25.040000000000003 120-124 20.3 30.42 24.26 25.019999999999996 125-129 19.994999999999997 31.09 24.865000000000002 24.05 130-134 21.795 31.025000000000002 23.645 23.535 135-139 22.145 30.0 24.795 23.06 140-144 22.675 30.014999999999997 25.465 21.845 145-149 20.794999999999998 30.630000000000003 24.62 23.955000000000002 150-151 20.5125 30.95 23.9 24.637500000000003 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.5 7 0.5 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.5 20 1.0 21 1.5 22 6.5 23 6.5 24 2.0 25 4.0 26 7.5 27 12.5 28 18.5 29 21.0 30 21.0 31 27.0 32 32.5 33 33.5 34 41.5 35 53.0 36 92.0 37 195.0 38 252.5 39 253.5 40 282.0 41 293.5 42 240.5 43 221.0 44 224.0 45 216.0 46 183.0 47 150.5 48 136.0 49 92.5 50 77.0 51 65.5 52 55.0 53 52.0 54 63.5 55 96.5 56 105.5 57 74.5 58 61.5 59 57.5 60 46.0 61 38.0 62 26.5 63 19.0 64 15.5 65 10.5 66 6.5 67 3.0 68 1.5 69 1.0 70 0.5 71 0.5 72 0.0 73 0.0 74 0.0 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.17500000000000002 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.015 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.034999999999999996 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.005 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.6 #Duplication Level Percentage of deduplicated Percentage of total 1 82.42694805194806 50.775000000000006 2 8.19805194805195 10.100000000000001 3 2.922077922077922 5.4 4 1.948051948051948 4.8 5 0.8928571428571428 2.75 6 1.2581168831168832 4.65 7 0.4058441558441558 1.7500000000000002 8 0.3246753246753247 1.6 9 0.12175324675324675 0.675 >10 1.461038961038961 15.950000000000001 >50 0.040584415584415584 1.55 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GCGGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTACG 62 1.55 No Hit GGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTACGTT 43 1.075 No Hit CTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCATTAAT 36 0.8999999999999999 No Hit GGCCAAAATAACCATGAGCAGCCACAATATTATAAGTCTCTTCCTCTTGA 36 0.8999999999999999 No Hit GCTTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCA 33 0.8250000000000001 No Hit CTTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCAT 30 0.75 No Hit GTGCTCTGCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTCCTA 23 0.575 No Hit GTTAGCGGAAAAACCTTATCCATTAATAGCGGGAACTTCAAGAGCAGCTA 22 0.5499999999999999 No Hit GACCCATATAACAAGCTACACCAAGTAAGAAGTGTAGAACAATTAGCTCA 22 0.5499999999999999 No Hit GCGACCTTGACTATCAACTACAGATTGGTTGAAATTGAATCCATTTAGGT 20 0.5 No Hit GCCAAAATAACCATGAGCAGCCACAATATTATAAGTCTCTTCCTCTTGAC 20 0.5 No Hit GTTGAAACTAGCATATTGGAAGATTAATCGGCCAAAATAACCATGAGCAG 19 0.475 No Hit GTGAGCATTACGTTCGTGCATTACTTCCATACCAAGATTAGCACGGTTGA 17 0.42500000000000004 No Hit GCGGAAAAACCTTATCCATTAATAGCGGGAACTTCAAGAGCAGCTAGATC 17 0.42500000000000004 No Hit GTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTC 16 0.4 No Hit GGAAAAACCTTATCCATTAATAGCGGGAACTTCAAGAGCAGCTAGATCTA 16 0.4 No Hit CTCATAAGGACCACCATTGTATAACCATTCATCAACGGATGCAGCTTCCC 15 0.375 No Hit GCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTCCTAAAGGCAT 15 0.375 No Hit CTCTGCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTCCTAAAG 15 0.375 No Hit GGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTACGTTC 15 0.375 No Hit GCTCTGCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTCCTAAA 15 0.375 No Hit CGGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTACGT 14 0.35000000000000003 No Hit CCCAAGTATTAATAACGCGACCTTGACTATCAACTACAGATTGGTTGAAA 14 0.35000000000000003 No Hit GGATGCATAAGGATGTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAA 14 0.35000000000000003 No Hit GGGAAGTTGTGAGCATTACGTTCGTGCATTACTTCCATACCAAGATTAGC 13 0.325 No Hit GCACGGTTGATGATATCAGCCCAAGTATTAATAACGCGACCTTGACTATC 12 0.3 No Hit CCCATATAACAAGCTACACCAAGTAAGAAGTGTAGAACAATTAGCTCATA 12 0.3 No Hit GGCATGATGACTTGGCCTCATCCTCTCCTTCCTCCGGCTTAACACCGGCG 12 0.3 No Hit CATATAACAAGCTACACCAAGTAAGAAGTGTAGAACAATTAGCTCATAAG 12 0.3 No Hit GCTGCGATGAAGGCGATAATAAATACAGAAGTTGCGGTCAATAAGGTAGG 12 0.3 No Hit GTTGATGATATCAGCCCAAGTATTAATAACGCGACCTTGACTATCAACTA 12 0.3 No Hit GATGCATAAGGATGTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAAA 12 0.3 No Hit GGGGCTGCGATGAAGGCGATAATAAATACAGAAGTTGCGGTCAATAAGGT 12 0.3 No Hit CCCTCATTAGCAGATTCATTTTCAGTAGTTTCCCTGATCAAACTAGAGGT 11 0.27499999999999997 No Hit GTTGAAATTGAATCCATTTAGGTTGAACGCCATAGTACTAATACCTAAAG 11 0.27499999999999997 No Hit CTGCGATGAAGGCGATAATAAATACAGAAGTTGCGGTCAATAAGGTAGGG 10 0.25 No Hit CTCGCGAATACCATCAATATCTACTGGAGGGGCTGCGATGAAGGCGATAA 10 0.25 No Hit GGCGATAATAAATACAGAAGTTGCGGTCAATAAGGTAGGGATCATCAAAA 9 0.22499999999999998 No Hit GCTAGTTATCCAGTTGCAGAAGCGACCCCACAGGCTTGTACTTTCGCGTC 9 0.22499999999999998 No Hit GAGCATTACGTTCGTGCATTACTTCCATACCAAGATTAGCACGGTTGATG 9 0.22499999999999998 No Hit GCCGAAAACACCAGCTACACCTAACATGTGAAATGGATGCATAAGGATGT 8 0.2 No Hit GAGGGAAGTTGTGAGCATTACGTTCGTGCATTACTTCCATACCAAGATTA 8 0.2 No Hit GCATATTGGAAGATTAATCGGCCAAAATAACCATGAGCAGCCACAATATT 8 0.2 No Hit CCACAATATTATAAGTCTCTTCCTCTTGACCAAATTTGTAACCCTCATTA 8 0.2 No Hit GCATAAGGATGTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAAAGTA 8 0.2 No Hit AGCGGAAAAACCTTATCCATTAATAGCGGGAACTTCAAGAGCAGCTAGAT 8 0.2 No Hit CCTCATTAGCAGATTCATTTTCAGTAGTTTCCCTGATCAAACTAGAGGTT 8 0.2 No Hit CCCCACTGCTGCCTCCCGTAGGAGTCTGGGCCGTGTCTCAGTCCCAGTGT 8 0.2 No Hit GTTGCGGTCAATAAGGTAGGGATCATCAAAACACCGAACCATCCGATGTA 7 0.17500000000000002 No Hit GTCAATTAGAAGAATAAAGAAGAATTACTGCATTTCGTAACTTATTTTTT 7 0.17500000000000002 No Hit GAGTTGTTGAAACTAGCATATTGGAAGATTAATCGGCCAAAATAACCATG 7 0.17500000000000002 No Hit CTCAGATACCGTCATTGTTTCTTCTCCGAGAAAAGAAGTTGACGACCCGT 7 0.17500000000000002 No Hit GCTAGATCTAGAGGGAAGTTGTGAGCATTACGTTCGTGCATTACTTCCAT 7 0.17500000000000002 No Hit GCCCAAGTATTAATAACGCGACCTTGACTATCAACTACAGATTGGTTGAA 7 0.17500000000000002 No Hit GTGAAATGGATGCATAAGGATGTTGTGCTCTGCCTGGAATACAATCATAA 7 0.17500000000000002 No Hit GGCGGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTAC 7 0.17500000000000002 No Hit CCGGGAACGGATTCACCGCCGTATGGCTGACCGGCGATTACTAGCGATTC 7 0.17500000000000002 No Hit CGCGACCTTGACTATCAACTACAGATTGGTTGAAATTGAATCCATTTAGG 7 0.17500000000000002 No Hit GCGGTCAATAAGGTAGGGATCATCAAAACACCGAACCATCCGATGTAAAG 6 0.15 No Hit CCGCAGAAGTAGGAATAATAGCACCAGAGATAATATTGTTTCCATAAAGT 6 0.15 No Hit CTTCTAACTTACCTACTACTGTACCGGCGTGGATATGATCTCCCCCAGAC 6 0.15 No Hit GGAGGGGCTGCGATGAAGGCGATAATAAATACAGAAGTTGCGGTCAATAA 6 0.15 No Hit CTTGACTATCAACTACAGATTGGTTGAAATTGAATCCATTTAGGTTGAAC 6 0.15 No Hit GTCATTGTTTCTTCTCCGAGAAAAGAAGTTGACGACCCGTAGGCCTTCCA 6 0.15 No Hit GGCGCATACCCAAACGGAAACTAAGTTCCCACTCACGACCCATATAACAA 6 0.15 No Hit CAGTGAACCAGATTCCTACTACAGGCCAAGCAGCCAAGAAGAAGTGTAAA 6 0.15 No Hit CTACTACAGGCCAAGCAGCCAAGAAGAAGTGTAAAGAACGAGAGTTGTTG 6 0.15 No Hit GCTGAATATGCAACAGCAATCCAAGGGCGCATACCCAAACGGAAACTAAG 6 0.15 No Hit TTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCATT 6 0.15 No Hit GAACCAGATTCCTACTACAGGCCAAGCAGCCAAGAAGAAGTGTAAAGAAC 6 0.15 No Hit CAATATTATAAGTCTCTTCCTCTTGACCAAATTTGTAACCCTCATTAGCA 6 0.15 No Hit CCAAGAAGAAGTGTAAAGAACGAGAGTTGTTGAAACTAGCATATTGGAAG 6 0.15 No Hit GCATTACGTTCGTGCATTACTTCCATACCAAGATTAGCACGGTTGATGAT 6 0.15 No Hit ATCGGCCAAAATAACCATGAGCAGCCACAATATTATAAGTCTCTTCCTCT 6 0.15 No Hit CCAGATTCCTACTACAGGCCAAGCAGCCAAGAAGAAGTGTAAAGAACGAG 6 0.15 No Hit TTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCATTAATA 6 0.15 No Hit CTAACATGTGAAATGGATGCATAAGGATGTTGTGCTCTGCCTGGAATACA 6 0.15 No Hit GGTCAATAAGGTAGGGATCATCAAAACACCGAACCATCCGATGTAAAGAC 6 0.15 No Hit ACGCGACCTTGACTATCAACTACAGATTGGTTGAAATTGAATCCATTTAG 6 0.15 No Hit GGAAGTTGTGAGCATTACGTTCGTGCATTACTTCCATACCAAGATTAGCA 6 0.15 No Hit GTACAAGGCCCGGGAACGGATTCACCGCCGTATGGCTGACCGGCGATTAC 6 0.15 No Hit CATCAATATCTACTGGAGGGGCTGCGATGAAGGCGATAATAAATACAGAA 6 0.15 No Hit CTCATTAGCAGATTCATTTTCAGTAGTTTCCCTGATCAAACTAGAGGTTA 6 0.15 No Hit CTCTTGACCAAATTTGTAACCCTCATTAGCAGATTCATTTTCAGTAGTTT 6 0.15 No Hit GTCCATGTACCAGTAGAAGATTCGGCAGCTACTGCAGCCCCTGCTTCTTC 6 0.15 No Hit CTCCACGCGGCATTGCTCCGTCAGGCTTTCGCCCATTGCGGAAAATTCCC 6 0.15 No Hit GGTAAATCAAGAAAACAGCAGTCGCAGCTGCAACAGGAGCTGAATATGCA 6 0.15 No Hit ATAACAAGCTACACCAAGTAAGAAGTGTAGAACAATTAGCTCATAAGGAC 6 0.15 No Hit GCCAAGAAGAAGTGTAAAGAACGAGAGTTGTTGAAACTAGCATATTGGAA 6 0.15 No Hit AGGATGTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAAAGTACCAGA 5 0.125 No Hit GGAAGATTAATCGGCCAAAATAACCATGAGCAGCCACAATATTATAAGTC 5 0.125 No Hit GTCGCAGCTGCAACAGGAGCTGAATATGCAACAGCAATCCAAGGGCGCAT 5 0.125 No Hit CAAGTATTAATAACGCGACCTTGACTATCAACTACAGATTGGTTGAAATT 5 0.125 No Hit TGATGATATCAGCCCAAGTATTAATAACGCGACCTTGACTATCAACTACA 5 0.125 No Hit GCTCATAAGGACCACCATTGTATAACCATTCATCAACGGATGCAGCTTCC 5 0.125 No Hit ACATGCTCCACCGCTTGTGCGGGCCCCCGTCAATTCCTTTGAGTTTCATT 5 0.125 No Hit CATCAGAGAAGCTTCCTTGACCAATAGGGTAAATCAAGAAAACAGCAGTC 5 0.125 No Hit GTAGGGATCATCAAAACACCGAACCATCCGATGTAAAGACGATTTTCAGT 5 0.125 No Hit CCCACTGCTGCCTCCCGTAGGAGTCTGGGCCGTGTCTCAGTCCCAGTGTG 5 0.125 No Hit GTTCGCTGGAGTAGGCTGCGAGGCCCAAGCGGCCAAGGAGTGCAGCGGCC 5 0.125 No Hit CTACAGGCCAAGCAGCCAAGAAGAAGTGTAAAGAACGAGAGTTGTTGAAA 5 0.125 No Hit GAAAAACCTTATCCATTAATAGCGGGAACTTCAAGAGCAGCTAGATCTAG 5 0.125 No Hit ACCAGAGATAATATTGTTTCCATAAAGTAAAGAACCAGAAACAGGCTCGC 5 0.125 No Hit GTCCCAGTGTGGCTGATCATCCTCTCGGACCAGCTACTGATCATCGCCTT 5 0.125 No Hit CCTCCACGCGGCATTGCTCCGTCAGGCTTTCGCCCATTGCGGAAAATTCC 5 0.125 No Hit CTAGCATATTGGAAGATTAATCGGCCAAAATAACCATGAGCAGCCACAAT 5 0.125 No Hit ATCAATATCTACTGGAGGGGCTGCGATGAAGGCGATAATAAATACAGAAG 5 0.125 No Hit CATAAGGATGTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAAAGTAC 5 0.125 No Hit GTAGGAATAATAGCACCAGAGATAATATTGTTTCCATAAAGTAAAGAACC 5 0.125 No Hit GCCAAGCAGCCAAGAAGAAGTGTAAAGAACGAGAGTTGTTGAAACTAGCA 5 0.125 No Hit GTTTCTTTTTATTTAGACCTTCTTCATATTTAGTTTTATCTATTAATCGA 5 0.125 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.025 0.0 0.0 0.0 0.0 58-59 0.025 0.0 0.0 0.0 0.0 60-61 0.025 0.0 0.0 0.0 0.0 62-63 0.025 0.0 0.0 0.0 0.0 64-65 0.025 0.0 0.0 0.0 0.0 66-67 0.05 0.0 0.0 0.0 0.0 68-69 0.075 0.0 0.0 0.0 0.0 70-71 0.075 0.0 0.0 0.0 0.0 72-73 0.075 0.0 0.0 0.0 0.0 74-75 0.0875 0.0 0.0 0.0 0.0 76-77 0.1125 0.0 0.0 0.0 0.0 78-79 0.1875 0.0 0.0 0.0 0.0 80-81 0.21250000000000002 0.0 0.0 0.0 0.0 82-83 0.30000000000000004 0.0 0.0 0.0 0.0 84-85 0.36250000000000004 0.0 0.0 0.0 0.0 86-87 0.4625 0.0 0.0 0.0 0.0 88-89 0.5625 0.0 0.0 0.0 0.0 90-91 0.7375 0.0 0.0 0.0 0.0 92-93 0.9125000000000001 0.0 0.0 0.0 0.0 94-95 1.0625 0.0 0.0 0.0 0.0 96-97 1.2125 0.0 0.0 0.0 0.0 98-99 1.3 0.0 0.0 0.0 0.0 100-101 1.4249999999999998 0.0 0.0 0.0 0.0 102-103 1.6 0.0 0.0 0.0 0.0 104-105 1.85 0.0 0.0 0.0 0.0 106-107 2.175 0.0 0.0 0.0 0.0 108-109 2.5125 0.0 0.0 0.0 0.0 110-111 2.7249999999999996 0.0 0.0 0.0 0.0 112-113 2.9875 0.0 0.0 0.0 0.0 114-115 3.3 0.0 0.0 0.0 0.0 116-117 3.575 0.0 0.0 0.0 0.0 118-119 3.9125 0.0 0.0 0.0 0.0 120-121 4.2125 0.0 0.0 0.0 0.0 122-123 4.487500000000001 0.0 0.0 0.0 0.0 124-125 4.775 0.0 0.0 0.0 0.0 126-127 5.2625 0.0 0.0 0.0 0.0 128-129 5.512499999999999 0.0 0.0 0.0 0.0 130-131 5.95 0.0 0.0 0.0 0.0 132-133 6.2625 0.0 0.0 0.0 0.0 134-135 6.699999999999999 0.0 0.0 0.0 0.0 136-137 7.1 0.0 0.0 0.0 0.0 138-139 7.487500000000001 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content pass >>END_MODULE SRR6941550 read2 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR6941550_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 34.007 35.0 35.0 35.0 33.0 35.0 2 34.0345 35.0 35.0 35.0 32.0 35.0 3 33.88975 35.0 35.0 35.0 32.0 35.0 4 34.142 35.0 35.0 35.0 33.0 35.0 5 34.185 35.0 35.0 35.0 33.0 35.0 6 38.66875 40.0 39.0 40.0 37.0 40.0 7 38.93025 40.0 40.0 40.0 38.0 40.0 8 38.939 40.0 40.0 40.0 38.0 40.0 9 38.973 40.0 40.0 40.0 38.0 40.0 10-14 38.82764999999999 40.0 40.0 40.0 37.6 40.0 15-19 38.6134 40.0 39.8 40.0 36.6 40.0 20-24 38.94615 40.0 40.0 40.0 38.0 40.0 25-29 38.884 40.0 40.0 40.0 37.8 40.0 30-34 38.8328 40.0 40.0 40.0 37.4 40.0 35-39 38.7937 40.0 39.8 40.0 37.4 40.0 40-44 38.896 40.0 40.0 40.0 38.2 40.0 45-49 38.6811 40.0 39.6 40.0 36.8 40.0 50-54 38.814249999999994 40.0 40.0 40.0 37.6 40.0 55-59 38.70985 40.0 39.6 40.0 37.0 40.0 60-64 38.826499999999996 40.0 40.0 40.0 37.8 40.0 65-69 38.67845 40.0 39.8 40.0 37.0 40.0 70-74 38.4439 40.0 39.0 40.0 36.0 40.0 75-79 38.7296 40.0 39.6 40.0 37.2 40.0 80-84 38.68315 40.0 39.0 40.0 37.0 40.0 85-89 38.4502 40.0 39.0 40.0 36.0 40.0 90-94 38.411500000000004 40.0 39.0 40.0 36.0 40.0 95-99 38.29260000000001 40.0 39.0 40.0 35.8 40.0 100-104 37.076100000000004 38.6 37.8 39.4 33.4 39.6 105-109 38.0095 40.0 39.0 40.0 34.6 40.0 110-114 37.984300000000005 40.0 39.0 40.0 35.0 40.0 115-119 37.756 40.0 39.0 40.0 33.8 40.0 120-124 37.75905 40.0 39.0 40.0 34.4 40.0 125-129 37.4072 40.0 38.8 40.0 32.6 40.0 130-134 37.8135 40.0 39.0 40.0 34.6 40.0 135-139 37.49765 40.0 39.0 40.0 33.8 40.0 140-144 37.2738 40.0 39.0 40.0 34.0 40.0 145-149 36.638400000000004 40.0 38.6 40.0 30.2 40.0 150-151 34.455625 38.5 35.0 39.5 23.0 40.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 7.0 3 1.0 4 0.0 5 0.0 6 6.0 7 0.0 8 0.0 9 0.0 10 0.0 11 1.0 12 0.0 13 0.0 14 1.0 15 1.0 16 3.0 17 3.0 18 2.0 19 2.0 20 4.0 21 5.0 22 12.0 23 14.0 24 9.0 25 17.0 26 22.0 27 22.0 28 28.0 29 27.0 30 29.0 31 41.0 32 50.0 33 60.0 34 67.0 35 95.0 36 129.0 37 170.0 38 376.0 39 2796.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.12717199697809 21.38000503651473 10.274490052883404 23.218332913623772 2 23.675621390911374 24.202862164197843 35.60130554858147 16.520210896309315 3 20.250312891113893 26.007509386733418 37.64705882352941 16.09511889862328 4 24.202060819301334 31.817039457150038 25.936164865544107 18.044734858004524 5 26.302605210420843 30.38577154308617 26.65330661322645 16.658316633266534 6 21.12393376818866 33.56748620170597 27.822378324134473 17.486201705970895 7 18.1248433191276 20.481323640010025 42.968162446728506 18.42567059413387 8 20.95118898623279 24.63078848560701 29.561952440550687 24.85607008760951 9 23.053817271589487 21.67709637046308 32.690863579474346 22.57822277847309 10-14 23.99539031967131 25.914420282593447 30.894879246417478 19.19531015131777 15-19 23.614941087991976 25.439959889696667 31.085485083980945 19.85961393833041 20-24 23.66167559717562 25.113926586208624 31.90945966247684 19.314938154138915 25-29 23.931880791384923 25.7450538442274 30.37816178312046 19.94490358126722 30-34 24.650813516896118 24.795994993742177 30.783479349186482 19.76971214017522 35-39 24.148637820512818 24.939903846153847 30.994591346153843 19.91686698717949 40-44 23.24335153002454 26.769169129062952 29.66394551009165 20.323533830820857 45-49 23.103500075108908 26.64864052876671 30.629412648340093 19.618446747784287 50-54 23.29110120687065 25.634733837448042 30.877860684060295 20.196304271621013 55-59 22.895488006409938 27.407481596474536 29.510741649556817 20.186288747558716 60-64 22.978318561914776 25.516999649491762 30.654448950978917 20.85023283761454 65-69 24.00721117732485 25.659772647603784 30.116680855325757 20.216335319745607 70-74 24.23377403846154 25.420673076923077 30.69911858974359 19.646434294871796 75-79 24.022038567493112 24.628099173553718 29.967443025294266 21.382419233658904 80-84 24.24014821491162 24.435431375494467 32.28180862250263 19.042611787091282 85-89 24.829761666332868 25.806128580012018 29.866813538954535 19.49729621470058 90-94 24.402824377785567 24.668235765436428 29.871300515799486 21.05763934097852 95-99 23.69461827284105 25.376720901126408 30.207759699624532 20.72090112640801 100-104 23.895270100817577 25.971811205296685 30.200130410794003 19.93278828309174 105-109 25.047743491808223 24.70097497235903 30.455322142928935 19.795959392903807 110-114 24.246079613992762 25.45737836751106 30.473462002412543 19.823080016083637 115-119 24.969873468567986 25.587467362924283 29.95581442056638 19.486844747941355 120-124 23.70102107539862 25.652633167345705 29.394899652934964 21.25144610432071 125-129 24.256258465860633 26.172678472884165 29.1877790598505 20.383284001404707 130-134 23.750439543879036 25.96071733561059 29.577535540262218 20.711307580248153 135-139 24.199145084234345 25.984410359567512 30.047774704551166 19.76866985164697 140-144 24.967287367891295 25.918470055359837 29.944640161046802 19.169602415702062 145-149 24.788817377312952 25.377111826226873 29.847144006436043 19.986926790024135 150-151 23.829252981795353 25.260514752040176 31.211550533584433 19.698681732580038 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 5.0 1 2.5 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.5 9 0.5 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.5 17 0.5 18 0.5 19 1.0 20 2.5 21 3.0 22 1.5 23 2.5 24 5.5 25 9.5 26 11.5 27 10.0 28 11.5 29 26.5 30 33.5 31 33.0 32 37.5 33 51.0 34 74.5 35 86.0 36 114.0 37 175.0 38 197.0 39 206.0 40 249.0 41 273.5 42 235.0 43 214.0 44 234.0 45 212.0 46 182.0 47 151.0 48 118.0 49 86.0 50 64.5 51 76.0 52 66.5 53 63.0 54 95.0 55 114.0 56 102.5 57 69.0 58 50.5 59 56.5 60 54.0 61 39.0 62 35.0 63 24.5 64 10.0 65 5.5 66 3.5 67 4.5 68 5.0 69 3.0 70 2.0 71 1.0 72 0.5 73 0.5 74 0.0 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.7250000000000001 2 0.42500000000000004 3 0.125 4 0.525 5 0.2 6 0.35000000000000003 7 0.27499999999999997 8 0.125 9 0.125 10-14 0.21 15-19 0.27499999999999997 20-24 0.155 25-29 0.17500000000000002 30-34 0.125 35-39 0.16 40-44 0.165 45-49 0.145 50-54 0.155 55-59 0.155 60-64 0.145 65-69 0.155 70-74 0.16 75-79 0.17500000000000002 80-84 0.145 85-89 0.13999999999999999 90-94 0.155 95-99 0.125 100-104 0.315 105-109 0.51 110-114 0.52 115-119 0.42 120-124 0.5950000000000001 125-129 0.335 130-134 0.46499999999999997 135-139 0.575 140-144 0.65 145-149 0.5599999999999999 150-151 0.43750000000000006 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 66.175 #Duplication Level Percentage of deduplicated Percentage of total 1 82.31960710238006 54.474999999999994 2 8.386853041178693 11.1 3 3.853418964865886 7.6499999999999995 4 1.2466943709860219 3.3000000000000003 5 1.0200226671703816 3.375 6 0.9444654325651681 3.75 7 0.415564790328674 1.925 8 0.34000755572346053 1.7999999999999998 9 0.5289006422364941 3.15 >10 0.9444654325651681 9.475 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTTCGATCCTGGCTCAGGATGAACGCTGGCGGCATGCTTAACACATGCAA 27 0.675 No Hit GCTTGTTATATGGGTCGTGAGTGGGAACTTAGTTTCCGTTTGGGTATGCG 24 0.6 No Hit ATTATCTATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAAT 24 0.6 No Hit GTTATTTTGGCCGATTAATCTTCCAATATGCTAGTTTCAACAACTCTCGT 23 0.575 No Hit GGAAGCTTCTCTGATGGTATGCCTTTAGGAATATCTGGTACTTTCAACTT 20 0.5 No Hit CTATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAA 18 0.44999999999999996 No Hit GCTAGTTTCAACAACTCTCGTTCTTTACACTTCTTCTTGGCTGCTTGGCC 17 0.42500000000000004 No Hit GTTATACAATGGTGGTCCTTATGAGCTAATTGTTCTACACTTCTTACTTG 16 0.4 No Hit TATCTATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTT 16 0.4 No Hit AGCACAACATCCTTATGCATCCATTTCACATGTTAGGTGTAGCTGGTGTT 14 0.35000000000000003 No Hit GTTTTGATGATCCCTACCTTATTGACCGCAACTTCTGTATTTATTATCGC 14 0.35000000000000003 No Hit GGAAACTACTGAAAATGAATCTGCTAATGAGGGTTACAAATTTGGTCAAG 13 0.325 No Hit GTGGGAACTTAGTTTCCGTTTGGGTATGCGCCCTTGGATTGCTGTTGCAT 13 0.325 No Hit GCACAACATCCTTATGCATCCATTTCACATGTTAGGTGTAGCTGGTGTTT 13 0.325 No Hit GTTCAACCTAAATGGATTCAATTTCAACCAATCTGTAGTTGATAGTCAAG 13 0.325 No Hit GTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGATCTTACCATGAC 13 0.325 No Hit ATCTATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTG 13 0.325 No Hit CTGGTACTTTCAACTTTATGATTGTATTCCAGGCAGAGCACAACATCCTT 12 0.3 No Hit ATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAA 12 0.3 No Hit ATTCAGTGCTATGCATGGTTCCTTGGTAACCTCTAGTTTGATCAGGGAAA 11 0.27499999999999997 No Hit AGGGAAACTACTGAAAATGAATCTGCTAATGAGGGTTACAAATTTGGTCA 11 0.27499999999999997 No Hit GTTAGGTGTAGCTGGTGTTTTCGGCGGTTCCCTATTCAGTGCTATGCATG 11 0.27499999999999997 No Hit GAAGCTTCTCTGATGGTATGCCTTTAGGAATATCTGGTACTTTCAACTTT 11 0.27499999999999997 No Hit GGGAAACTACTGAAAATGAATCTGCTAATGAGGGTTACAAATTTGGTCAA 10 0.25 No Hit CAGGGAAACTACTGAAAATGAATCTGCTAATGAGGGTTACAAATTTGGTC 10 0.25 No Hit CCTAAATGGATTCAATTTCAACCAATCTGTAGTTGATAGTCAAGGTCGCG 9 0.22499999999999998 No Hit GTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGATCTT 9 0.22499999999999998 No Hit GAGGGTTACAAATTTGGTCAAGAGGAAGAGACTTATAATATTGTGGCTGC 9 0.22499999999999998 No Hit ATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCTAAATGG 9 0.22499999999999998 No Hit GTTGATAGTCAAGGTCGCGTTATTAATACTTGGGCTGATATCATCAACCG 9 0.22499999999999998 No Hit CTTAGTTTCCGTTTGGGTATGCGCCCTTGGATTGCTGTTGCATATTCAGC 9 0.22499999999999998 No Hit GTTATTGTGAGAATTCTTAATTCAAGAGTTGTAAGGAGGGACTTATGTCA 9 0.22499999999999998 No Hit TGTAGCTGGTGTTTTCGGCGGTTCCCTATTCAGTGCTATGCATGGTTCCT 9 0.22499999999999998 No Hit CAATGGTGGTCCTTATGAGCTAATTGTTCTACACTTCTTACTTGGTGTAG 9 0.22499999999999998 No Hit CAGTGACCAGGCCCGGGCGACTGTTTACCAAAAACACAGGTCTCCGCAAA 9 0.22499999999999998 No Hit GTTTCTGGTTCTTTACTTTATGGAAACAATATTATCTCTGGTGCTATTAT 9 0.22499999999999998 No Hit GCTGCGACTGCTGTTTTCTTGATTTACCCTATTGGTCAAGGAAGCTTCTC 9 0.22499999999999998 No Hit GAATGGTTATACAATGGTGGTCCTTATGAGCTAATTGTTCTACACTTCTT 9 0.22499999999999998 No Hit AGAACCTTACCAGGGCTTGACATGCCGCGAATCCTCTTGAAAGAGAGGGG 9 0.22499999999999998 No Hit GGAAGAGACTTATAATATTGTGGCTGCTCATGGTTATTTTGGCCGATTAA 8 0.2 No Hit GTCGTGAGTGGGAACTTAGTTTCCGTTTGGGTATGCGCCCTTGGATTGCT 8 0.2 No Hit CTCTAGTTTGATCAGGGAAACTACTGAAAATGAATCTGCTAATGAGGGTT 8 0.2 No Hit ATTTGGTCAAGAGGAAGAGACTTATAATATTGTGGCTGCTCATGGTTATT 8 0.2 No Hit GCTGTTTTCTTGATTTACCCTATTGGTCAAGGAAGCTTCTCTGATGGTAT 8 0.2 No Hit GGAATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCTAAA 8 0.2 No Hit GTTGCATATTCAGCTCCTGTTGCAGCTGCGACTGCTGTTTTCTTGATTTA 8 0.2 No Hit GGGAACTTAGTTTCCGTTTGGGTATGCGCCCTTGGATTGCTGTTGCATAT 8 0.2 No Hit GGTACTTTCAACTTTATGATTGTATTCCAGGCAGAGCACAACATCCTTAT 8 0.2 No Hit CTTTTACCCAATTTGGGAAGCTGCATCCGTTGATGAATGGTTATACAATG 7 0.17500000000000002 No Hit ATCGGATTGCACTTTTACCCAATTTGGGAAGCTGCATCCGTTGATGAATG 7 0.17500000000000002 No Hit AAATTTGGTCAAGAGGAAGAGACTTATAATATTGTGGCTGCTCATGGTTA 7 0.17500000000000002 No Hit GTTCCTTGGTAACCTCTAGTTTGATCAGGGAAACTACTGAAAATGAATCT 7 0.17500000000000002 No Hit TATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAAT 7 0.17500000000000002 No Hit CGTTGATGAATGGTTATACAATGGTGGTCCTTATGAGCTAATTGTTCTAC 7 0.17500000000000002 No Hit GCATGGTTCCTTGGTAACCTCTAGTTTGATCAGGGAAACTACTGAAAATG 7 0.17500000000000002 No Hit GGCTTGCGGTGGATACCTAGGCACCCAGAGACGAGGAAGGGCGTAGCAAG 7 0.17500000000000002 No Hit GTAGGAATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCT 7 0.17500000000000002 No Hit GAAGAGACTTATAATATTGTGGCTGCTCATGGTTATTTTGGCCGATTAAT 7 0.17500000000000002 No Hit GTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGATCTTACCATGACTG 7 0.17500000000000002 No Hit CATGGTTATTTTGGCCGATTAATCTTCCAATATGCTAGTTTCAACAACTC 6 0.15 No Hit GCTTCTGCAACTGGATAACTAGCACTGAAAATCGTCTTTACATCGGATGG 6 0.15 No Hit CTGCTTTAGGTATTAGTACTATGGCGTTCAACCTAAATGGATTCAATTTC 6 0.15 No Hit GAACCTTACCAGGGCTTGACATGCCGCGAATCCTCTTGAAAGAGAGGGGT 6 0.15 No Hit CAACTCTCGTTCTTTACACTTCTTCTTGGCTGCTTGGCCTGTAGTAGGAA 6 0.15 No Hit GCAACTTCTGTATTTATTATCGCCTTCATCGCAGCCCCTCCAGTAGATAT 6 0.15 No Hit GCTTGGGAGTCCTTGCAATTTGAATAAACCAAGATCTTACCATGACTGCA 6 0.15 No Hit CCTGGCTCAGGATGAACGCTGGCGGCATGCTTAACACATGCAAGTCGAAC 6 0.15 No Hit GATTAATCTTCCAATATGCTAGTTTCAACAACTCTCGTTCTTTACACTTC 6 0.15 No Hit TGGGAACTTAGTTTCCGTTTGGGTATGCGCCCTTGGATTGCTGTTGCATA 6 0.15 No Hit GAGACTTATAATATTGTGGCTGCTCATGGTTATTTTGGCCGATTAATCTT 6 0.15 No Hit CTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCTAAATGGAT 6 0.15 No Hit GATGAACGCTGGCGGCATGCTTAACACATGCAAGTCGAACGGGAAGTGGT 6 0.15 No Hit TGAAAATCGTCTTTACATCGGATGGTTCGGTGTTTTGATGATCCCTACCT 6 0.15 No Hit GTGCTATGCATGGTTCCTTGGTAACCTCTAGTTTGATCAGGGAAACTACT 6 0.15 No Hit GGATGGTTCGGTGTTTTGATGATCCCTACCTTATTGACCGCAACTTCTGT 6 0.15 No Hit CTAGCACTGAAAATCGTCTTTACATCGGATGGTTCGGTGTTTTGATGATC 6 0.15 No Hit ATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCA 6 0.15 No Hit GTTTCAACAACTCTCGTTCTTTACACTTCTTCTTGGCTGCTTGGCCTGTA 6 0.15 No Hit GTCCTTGCAATTTGAATAAACCAAGATCTTACCATGACTGCAATTTTAGA 6 0.15 No Hit GCTGCATCCGTTGATGAATGGTTATACAATGGTGGTCCTTATGAGCTAAT 6 0.15 No Hit TCTATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGA 6 0.15 No Hit CTTCCAATATGCTAGTTTCAACAACTCTCGTTCTTTACACTTCTTCTTGG 6 0.15 No Hit CTGAAAATGAATCTGCTAATGAGGGTTACAAATTTGGTCAAGAGGAAGAG 6 0.15 No Hit GAGACGCGAAAGTACAAGCCTGTGGGGTCGCTTCTGCAACTGGATAACTA 6 0.15 No Hit GGTAACCTCTAGTTTGATCAGGGAAACTACTGAAAATGAATCTGCTAATG 5 0.125 No Hit CCAATATGCTAGTTTCAACAACTCTCGTTCTTTACACTTCTTCTTGGCTG 5 0.125 No Hit GTGGCTGCTCATGGTTATTTTGGCCGATTAATCTTCCAATATGCTAGTTT 5 0.125 No Hit TATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAA 5 0.125 No Hit GGCCTGTAGTAGGAATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCG 5 0.125 No Hit GCTAATGAGGGTTACAAATTTGGTCAAGAGGAAGAGACTTATAATATTGT 5 0.125 No Hit CTGCTAATGAGGGTTACAAATTTGGTCAAGAGGAAGAGACTTATAATATT 5 0.125 No Hit CATGACTGCAATTTTAGAGAGACGCGAAAGTACAAGCCTGTGGGGTCGCT 5 0.125 No Hit AGGGTTACAAATTTGGTCAAGAGGAAGAGACTTATAATATTGTGGCTGCT 5 0.125 No Hit ATCTGGTACTTTCAACTTTATGATTGTATTCCAGGCAGAGCACAACATCC 5 0.125 No Hit AATGAATCTGCTAATGAGGGTTACAAATTTGGTCAAGAGGAAGAGACTTA 5 0.125 No Hit GGAACAACAACTGGAAACGGTTGCTAATACCCCGTAGGCTGAGGAGCAAA 5 0.125 No Hit GTTATATGGGTCGTGAGTGGGAACTTAGTTTCCGTTTGGGTATGCGCCCT 5 0.125 No Hit ATTATCTCTGGTGCTATTATTCCTACTTCTGCGGCAATCGGATTGCACTT 5 0.125 No Hit ATTTCACATGTTAGGTGTAGCTGGTGTTTTCGGCGGTTCCCTATTCAGTG 5 0.125 No Hit GGAAACAATATTATCTCTGGTGCTATTATTCCTACTTCTGCGGCAATCGG 5 0.125 No Hit GAGGAAGAGACTTATAATATTGTGGCTGCTCATGGTTATTTTGGCCGATT 5 0.125 No Hit CATCCTTATGCATCCATTTCACATGTTAGGTGTAGCTGGTGTTTTCGGCG 5 0.125 No Hit CCTTACCAGGGCTTGACATGCCGCGAATCCTCTTGAAAGAGAGGGGTGCC 5 0.125 No Hit GTACAAGCCTGTGGGGTCGCTTCTGCAACTGGATAACTAGCACTGAAAAT 5 0.125 No Hit GTTTGATCAGGGAAACTACTGAAAATGAATCTGCTAATGAGGGTTACAAA 5 0.125 No Hit GTTCTACACTTCTTACTTGGTGTAGCTTGTTATATGGGTCGTGAGTGGGA 5 0.125 No Hit GCACTTTTACCCAATTTGGGAAGCTGCATCCGTTGATGAATGGTTATACA 5 0.125 No Hit CTTTATGATTGTATTCCAGGCAGAGCACAACATCCTTATGCATCCATTTC 5 0.125 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 5 0.125 No Hit TTCAGCTCCTGTTGCAGCTGCGACTGCTGTTTTCTTGATTTACCCTATTG 5 0.125 No Hit AGCTTGTTATATGGGTCGTGAGTGGGAACTTAGTTTCCGTTTGGGTATGC 5 0.125 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.025 0.0 0.0 0.0 0.0 58-59 0.025 0.0 0.0 0.0 0.0 60-61 0.025 0.0 0.0 0.0 0.0 62-63 0.025 0.0 0.0 0.0 0.0 64-65 0.025 0.0 0.0 0.0 0.0 66-67 0.05 0.0 0.0 0.0 0.0 68-69 0.075 0.0 0.0 0.0 0.0 70-71 0.075 0.0 0.0 0.0 0.0 72-73 0.075 0.0 0.0 0.0 0.0 74-75 0.0875 0.0 0.0 0.0 0.0 76-77 0.1125 0.0 0.0 0.0 0.0 78-79 0.1875 0.0 0.0 0.0 0.0 80-81 0.21250000000000002 0.0 0.0 0.0 0.0 82-83 0.30000000000000004 0.0 0.0 0.0 0.0 84-85 0.36250000000000004 0.0 0.0 0.0 0.0 86-87 0.475 0.0 0.0 0.0 0.0 88-89 0.5875 0.0 0.0 0.0 0.0 90-91 0.7625 0.0 0.0 0.0 0.0 92-93 0.9375 0.0 0.0 0.0 0.0 94-95 1.0875 0.0 0.0 0.0 0.0 96-97 1.2374999999999998 0.0 0.0 0.0 0.0 98-99 1.325 0.0 0.0 0.0 0.0 100-101 1.4500000000000002 0.0 0.0 0.0 0.0 102-103 1.6124999999999998 0.0 0.0 0.0 0.0 104-105 1.85 0.0 0.0 0.0 0.0 106-107 2.1375 0.0 0.0 0.0 0.0 108-109 2.4625 0.0 0.0 0.0 0.0 110-111 2.6625 0.0 0.0 0.0 0.0 112-113 2.925 0.0 0.0 0.0 0.0 114-115 3.2 0.0 0.0 0.0 0.0 116-117 3.4875 0.0 0.0 0.0 0.0 118-119 3.8375 0.0 0.0 0.0 0.0 120-121 4.1375 0.0 0.0 0.0 0.0 122-123 4.4375 0.0 0.0 0.0 0.0 124-125 4.699999999999999 0.0 0.0 0.0 0.0 126-127 5.1625 0.0 0.0 0.0 0.0 128-129 5.4125 0.0 0.0 0.0 0.0 130-131 5.875 0.0 0.0 0.0 0.0 132-133 6.2125 0.0 0.0 0.0 0.0 134-135 6.65 0.0 0.0 0.0 0.0 136-137 7.025 0.0 0.0 0.0 0.0 138-139 7.3875 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content pass >>END_MODULE Read 1261851 spots for SRR6941550.sra Written 1261851 spots for SRR6941550.sra Read 1261851 spots for SRR6941550.sra Written 1261851 spots for SRR6941550.sra Read 1261851 spots for SRR6941550.sra Written 1261851 spots for SRR6941550.sra Read 1261851 spots for SRR6941550.sra Written 1261851 spots for SRR6941550.sra Read 1261851 spots for SRR6941550.sra Written 1261851 spots for SRR6941550.sra Read 1261851 spots for SRR6941550.sra Written 1261851 spots for SRR6941550.sra Read 1261851 spots for SRR6941550.sra Written 1261851 spots for SRR6941550.sra Read 1261851 spots for SRR6941550.sra Written 1261851 spots for SRR6941550.sra Read 1261851 spots for SRR6941550.sra Written 1261851 spots for SRR6941550.sra Read 1261851 spots for SRR6941550.sra Written 1261851 spots for SRR6941550.sra Read 1261851 spots for SRR6941550.sra Written 1261851 spots for SRR6941550.sra Read 1261851 spots for SRR6941550.sra Written 1261851 spots for SRR6941550.sra Read 1261866 spots for SRR6941550.sra Written 1261866 spots for SRR6941550.sra Read 1261851 spots for SRR6941550.sra Written 1261851 spots for SRR6941550.sra Read 1261851 spots for SRR6941550.sra Written 1261851 spots for SRR6941550.sra Read 1261851 spots for SRR6941550.sra Written 1261851 spots for SRR6941550.sra Read 1261851 spots for SRR6941550.sra Written 1261851 spots for SRR6941550.sra Read 1261851 spots for SRR6941550.sra Written 1261851 spots for SRR6941550.sra Read 1261851 spots for SRR6941550.sra Written 1261851 spots for SRR6941550.sra Read 1261851 spots for SRR6941550.sra Written 1261851 spots for SRR6941550.sra SRR ids: ['SRR6941550.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_bd63pjjk SRR6941550.sra spots: 25237035 blocks: [[1, 1261851], [1261852, 2523702], [2523703, 3785553], [3785554, 5047404], [5047405, 6309255], [6309256, 7571106], [7571107, 8832957], [8832958, 10094808], [10094809, 11356659], [11356660, 12618510], [12618511, 13880361], [13880362, 15142212], [15142213, 16404063], [16404064, 17665914], [17665915, 18927765], [18927766, 20189616], [20189617, 21451467], [21451468, 22713318], [22713319, 23975169], [23975170, 25237035]] SRR6941550 file size 8530302 SRR6941550 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR6941550 SRR6941550_1.fastq SRR6941550_2.fastq Input file: SRR6941550_1.fastq Paired file: SRR6941550_2.fastq trimmed: SRR6941550-trimmed-pair1.fastq, SRR6941550-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Fri Dec 6 11:04:09 2024 >> started Fri Dec 6 11:04:38 2024 >> done (28.942s) 25237035 read pairs processed; of these: 11271 ( 0.04%) short read pairs filtered out after trimming by size control 27915 ( 0.11%) empty read pairs filtered out after trimming by size control 25197849 (99.84%) read pairs available; of these: 4628736 (18.37%) trimmed read pairs available after processing 20569113 (81.63%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 1 0.00% 19 1 0.00% 20 3 0.00% 21 5 0.00% 22 5 0.00% 23 1 0.00% 24 5 0.00% 25 4 0.00% 26 6 0.00% 27 4 0.00% 28 14 0.00% 29 9 0.00% 30 16 0.00% 31 11 0.00% 32 14 0.00% 33 19 0.00% 34 12 0.00% 35 19 0.00% 36 32 0.00% 37 31 0.00% 38 37 0.00% 39 50 0.00% 40 61 0.00% 41 60 0.00% 42 84 0.00% 43 80 0.00% 44 86 0.00% 45 91 0.00% 46 94 0.00% 47 122 0.00% 48 153 0.00% 49 171 0.00% 50 224 0.00% 51 238 0.00% 52 327 0.00% 53 317 0.00% 54 408 0.00% 55 402 0.00% 56 462 0.00% 57 544 0.00% 58 607 0.00% 59 666 0.00% 60 828 0.00% 61 1009 0.00% 62 1308 0.01% 63 1399 0.01% 64 1684 0.01% 65 1812 0.01% 66 1947 0.01% 67 2084 0.01% 68 2504 0.01% 69 2867 0.01% 70 3215 0.01% 71 3706 0.01% 72 4502 0.02% 73 4850 0.02% 74 4936 0.02% 75 5789 0.02% 76 5768 0.02% 77 6810 0.03% 78 6561 0.03% 79 7171 0.03% 80 8302 0.03% 81 9134 0.04% 82 10649 0.04% 83 10711 0.04% 84 11837 0.05% 85 14243 0.06% 86 14327 0.06% 87 15110 0.06% 88 17364 0.07% 89 16956 0.07% 90 19283 0.08% 91 19930 0.08% 92 22909 0.09% 93 23414 0.09% 94 24811 0.10% 95 26263 0.10% 96 24886 0.10% 97 24590 0.10% 98 24487 0.10% 99 25739 0.10% 100 26543 0.11% 101 29287 0.12% 102 32307 0.13% 103 32398 0.13% 104 34292 0.14% 105 36050 0.14% 106 36148 0.14% 107 35981 0.14% 108 36904 0.15% 109 40012 0.16% 110 39368 0.16% 111 43509 0.17% 112 45291 0.18% 113 42982 0.17% 114 46439 0.18% 115 43198 0.17% 116 44795 0.18% 117 42526 0.17% 118 42734 0.17% 119 42836 0.17% 120 44432 0.18% 121 45427 0.18% 122 51397 0.20% 123 54043 0.21% 124 54747 0.22% 125 56414 0.22% 126 52541 0.21% 127 52916 0.21% 128 53299 0.21% 129 58585 0.23% 130 57488 0.23% 131 61660 0.24% 132 61548 0.24% 133 55929 0.22% 134 64656 0.26% 135 61433 0.24% 136 65576 0.26% 137 63545 0.25% 138 70762 0.28% 139 72126 0.29% 140 68583 0.27% 141 83420 0.33% 142 72871 0.29% 143 81095 0.32% 144 78478 0.31% 145 91650 0.36% 146 99587 0.40% 147 106813 0.42% 148 135158 0.54% 149 195527 0.78% 150 1242239 4.93% 151 20569113 81.63% 25197849 reads passed initial QC criterion=sequence-density sequence-density=0.27 sequence-density-rank=1 fanout-score=1.98 fanout-score-rank=31 prefix-density=0.27 prefix-fanout=2.0 sequence=AATATACCCAATG criterion=fanout-score sequence-density=0.01 sequence-density-rank=36 fanout-score=60.82 fanout-score-rank=1 prefix-density=0.08 prefix-fanout=5.9 sequence=AAAAAAAAAGGGGGGTAAGGACCCGCTAAGCTCCTACTTTTTCATGTTTCCAATCCGATCCCTCCGATTACTATAGAGATGAACCCAATCCAGAATATGAACCATAAAAGAAAACACCTACTAAACCAATCACAAGAATACCAGTTACCGTACCTATCAGCCAAAGAGGAATTCTTCCAGTAGTATCGGCCATTTCCCCTACTTTCCTCCACATTTTATCAAGTGGTCATGCTAGAGACAAAAACAGTCATGGATAGTTATGTTATAAGGATGGTATCCTTCCAAATGGGATAAGAGAGTTCTTACTACTCTCTTCTTTTCTCTCAATTAAAGAAGTAATTGGAAAACAAAACAGCAAGTACAAAAATGAGTAATAAACCCCAGTATAGACTGGTACGATTCAATTCAACATTTTGTTCATTCGGGTTTGATTGTGTCATAGTTCTATAGTTGGAATTTAGTTTATCGTTGGATGAACTGCATTGCTGATATTGATCCCAAGAAAAAAACA criterion=sequence-density sequence-density=0.28 sequence-density-rank=1 fanout-score=2.00 fanout-score-rank=27 prefix-density=0.28 prefix-fanout=2.0 sequence=GTAGTAATTCTAG criterion=fanout-score sequence-density=0.06 sequence-density-rank=25 fanout-score=25.27 fanout-score-rank=1 prefix-density=1.44 prefix-fanout=1.1 sequence=TGGTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGTTAACGAACGAGACCTCAGCCTGCTAACTAGCTATGCGGAGCCATCCCTCCGCAGCTAGCTTCTTAGAGGGACTATCGCCGTTTAGGCGACGGAAGTTTGAGGCAATAACAGGTCTGTGATGCCCTTAGATGTTCTGGGCCGCACGCGCGCTACACTGATGTATTCAACGAGTATATAGCCTTGGCCGACAGGCCCGGGTAATCTTGGGAAATTTCATCGTGATGGGGATAGATCATTGCAATTGTTGGTCTTCAACGAGGAATGCCTAGTAAGCGCGAGTCATCAGCTCGCGTTGACTACGTCCCTGCCCTTTGTACACACC SRR6941550 testing PE reads STAR mapping to Ensembl genome Started job on | Dec 06 11:05:13 Started mapping on | Dec 06 11:05:13 Finished on | Dec 06 11:07:09 Mapping speed, Million of reads per hour | 782.00 Number of input reads | 25197849 Average input read length | 294 UNIQUE READS: Uniquely mapped reads number | 14527609 Uniquely mapped reads % | 57.65% Average mapped length | 295.86 Number of splices: Total | 2707646 Number of splices: Annotated (sjdb) | 2384383 Number of splices: GT/AG | 2509881 Number of splices: GC/AG | 31409 Number of splices: AT/AC | 13829 Number of splices: Non-canonical | 152527 Mismatch rate per base, % | 0.25% Deletion rate per base | 0.02% Deletion average length | 1.94 Insertion rate per base | 0.02% Insertion average length | 2.63 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 9029457 % of reads mapped to multiple loci | 35.83% Number of reads mapped to too many loci | 56597 % of reads mapped to too many loci | 0.22% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 5.17% % of reads unmapped: other | 1.12% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 1649024 1649024 1649024 N_multimapping 9029457 9029457 9029457 N_noFeature 6625512 13980178 6869019 N_ambiguous 579113 8828 283090 UnstrandedReadsAssigned:7322984 PositiveStrandReadsAssigned:538603 NegativeStrandReadsAssigned:7375500 Dataset is classified negative stranded MeadianReadLen=151 20thPercentileLength=151 echo kmer=147 SRR6941550 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,972 [index] number of k-mers: 66,720,672 [index] number of equivalence classes: 111,837 [quant] running in paired-end mode [quant] will process pair 1: SRR6941550-trimmed-pair1.fastq SRR6941550-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 25,197,849 reads, 13,319,914 reads pseudoaligned [quant] estimated average fragment length: 236.371 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 999 rounds 52973 SRR6941550.ke.tsv 35125 SRR6941550.se.tsv 88098 total ==> SRR6941550.ke.tsv <== target_id length eff_length est_counts tpm PNS24245 936 701.021 0 0 PNS24247 1044 808.629 6.09837 0.46873 PNS24249 1928 1692.63 0 0 PNS24246 1044 808.629 6.09837 0.46873 PNS24248 1044 808.629 6.09837 0.46873 PNS24244 1471 1235.63 28.7049 1.44386 PNS24243 293 100.995 0 0 KQK14069 1603 1367.63 230.967 10.4964 KQK14071 474 248.273 4.07376 1.01982 ==> SRR6941550.se.tsv <== BRADI_1g14170v3 335 BRADI_1g53295v3 33 BRADI_1g59795v3 12 BRADI_1g07683v3 0 BRADI_1g00485v3 1 BRADI_1g20270v3 84 BRADI_1g74790v3 23 BRADI_1g09890v3 0 BRADI_1g77505v3 30 BRADI_1g48960v3 0 SRR6941550 completed mapping pipeline successfully