Starting /dee2/code/volunteer_pipeline.sh SRR6941551 current disk space = 1551549030400 free memory = 1326856780 SRR6941551 SRAfilesize 38875038d09dc6addfa6c24fe87d461b SRR6941551.sra SRR6941551.sra file validated SRR6941551 is paired end SRR6941551 is conventional basespace SRR6941551 read1 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR6941551_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 34.617 35.0 35.0 35.0 35.0 35.0 2 34.59025 35.0 35.0 35.0 35.0 35.0 3 34.6315 35.0 35.0 35.0 35.0 35.0 4 34.692 35.0 35.0 35.0 35.0 35.0 5 34.6395 35.0 35.0 35.0 35.0 35.0 6 39.5035 40.0 40.0 40.0 39.0 40.0 7 39.49075 40.0 40.0 40.0 39.0 40.0 8 39.51625 40.0 40.0 40.0 39.0 40.0 9 39.50925 40.0 40.0 40.0 39.0 40.0 10-14 39.49675 40.0 40.0 40.0 39.0 40.0 15-19 39.438 40.0 40.0 40.0 39.0 40.0 20-24 39.4792 40.0 40.0 40.0 39.0 40.0 25-29 39.45795 40.0 40.0 40.0 39.0 40.0 30-34 39.294000000000004 40.0 40.0 40.0 38.8 40.0 35-39 39.4463 40.0 40.0 40.0 39.0 40.0 40-44 39.40725 40.0 40.0 40.0 39.0 40.0 45-49 39.383050000000004 40.0 40.0 40.0 39.0 40.0 50-54 39.3325 40.0 40.0 40.0 39.0 40.0 55-59 39.29834999999999 40.0 40.0 40.0 39.0 40.0 60-64 39.32835 40.0 40.0 40.0 39.0 40.0 65-69 39.27485 40.0 40.0 40.0 39.0 40.0 70-74 39.2242 40.0 40.0 40.0 38.8 40.0 75-79 39.100350000000006 40.0 40.0 40.0 38.4 40.0 80-84 39.16955 40.0 40.0 40.0 38.6 40.0 85-89 39.138999999999996 40.0 40.0 40.0 38.6 40.0 90-94 39.0581 40.0 39.6 40.0 38.2 40.0 95-99 39.0869 40.0 40.0 40.0 38.2 40.0 100-104 38.181799999999996 39.2 38.4 39.4 36.6 39.8 105-109 39.0998 40.0 39.6 40.0 38.2 40.0 110-114 39.161150000000006 40.0 40.0 40.0 38.6 40.0 115-119 39.2111 40.0 40.0 40.0 38.8 40.0 120-124 38.98965 40.0 39.8 40.0 37.8 40.0 125-129 38.897149999999996 40.0 39.0 40.0 37.4 40.0 130-134 38.89695 40.0 39.0 40.0 37.4 40.0 135-139 38.75295 40.0 39.0 40.0 36.8 40.0 140-144 38.6104 40.0 39.0 40.0 36.2 40.0 145-149 38.7441 40.0 39.0 40.0 37.0 40.0 150-151 37.068875000000006 39.5 37.5 40.0 33.0 40.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 24 2.0 25 3.0 26 8.0 27 8.0 28 13.0 29 19.0 30 14.0 31 24.0 32 29.0 33 39.0 34 49.0 35 71.0 36 83.0 37 142.0 38 307.0 39 3189.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 47.12960641764853 11.13060917523189 6.1418901980446226 35.59789420907495 2 23.25 13.8 32.85 30.099999999999998 3 21.075 18.75 26.950000000000003 33.225 4 26.400000000000002 25.650000000000002 21.75 26.200000000000003 5 24.223446893787575 32.890781563126254 23.12124248496994 19.76452905811623 6 19.625 37.85 23.425 19.1 7 14.475 30.599999999999998 39.7 15.225 8 16.725 28.975 31.7 22.6 9 16.525000000000002 24.45 37.75 21.275 10-14 18.55 35.115 25.46 20.875 15-19 19.875 31.485000000000003 26.13 22.509999999999998 20-24 18.15590779538977 31.886594329716484 27.431371568578427 22.526126306315316 25-29 21.475 32.12 26.16 20.244999999999997 30-34 21.59 32.595 24.54 21.275 35-39 20.830000000000002 31.96 26.6 20.61 40-44 18.85 31.715 27.084999999999997 22.35 45-49 19.175 31.125000000000004 27.785 21.915000000000003 50-54 20.05 31.740000000000002 26.305 21.905 55-59 20.31 29.975 25.855 23.86 60-64 18.715 31.574999999999996 27.310000000000002 22.400000000000002 65-69 19.185 31.53 26.950000000000003 22.335 70-74 20.175 31.39 24.745 23.69 75-79 20.13 30.195 27.01 22.665 80-84 21.52 30.835 25.665 21.98 85-89 21.15 30.425 25.83 22.595000000000002 90-94 18.685 31.645 27.229999999999997 22.439999999999998 95-99 19.765 31.555 25.019999999999996 23.66 100-104 19.7 32.714999999999996 25.245 22.34 105-109 19.255 31.564999999999998 26.88 22.3 110-114 20.849999999999998 29.695 26.87 22.585 115-119 19.509999999999998 31.75 24.959999999999997 23.78 120-124 19.687875150060023 30.77230892356943 25.130052020808325 24.409763905562226 125-129 19.75598779938997 31.856592829641485 25.03625181259063 23.35116755837792 130-134 21.01 31.979999999999997 23.96 23.05 135-139 21.46 31.169999999999998 24.805 22.564999999999998 140-144 22.105 30.680000000000003 26.0 21.215 145-149 19.88 31.78 25.224999999999998 23.115 150-151 19.45418127190786 32.97446169253881 23.823234852278418 23.748122183274912 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.5 19 0.5 20 1.0 21 2.0 22 5.0 23 7.0 24 8.0 25 9.5 26 11.0 27 10.5 28 13.0 29 24.0 30 28.5 31 27.5 32 34.5 33 39.0 34 44.0 35 58.5 36 109.0 37 227.5 38 278.5 39 243.0 40 277.5 41 320.5 42 268.5 43 256.5 44 267.0 45 243.0 46 203.5 47 161.0 48 139.0 49 102.0 50 78.5 51 58.0 52 41.5 53 34.5 54 39.5 55 51.0 56 49.0 57 40.5 58 35.5 59 29.5 60 29.5 61 24.5 62 14.0 63 8.5 64 11.0 65 16.5 66 8.5 67 1.5 68 2.0 69 2.0 70 1.5 71 0.5 72 1.0 73 1.0 74 0.0 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.27499999999999997 2 0.0 3 0.0 4 0.0 5 0.2 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.005 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.04 125-129 0.005 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.15 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 64.17500000000001 #Duplication Level Percentage of deduplicated Percentage of total 1 83.40475262952863 53.525 2 8.180755746007012 10.5 3 3.1554343591741336 6.075 4 1.4413712504869498 3.6999999999999997 5 0.7012076353720296 2.25 6 0.6622516556291391 2.55 7 0.23373587845734323 1.05 8 0.46747175691468645 2.4 9 0.3506038176860148 2.025 >10 1.3634592910011687 14.524999999999999 >50 0.038955979742890535 1.4000000000000001 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GCGGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTACG 56 1.4000000000000001 No Hit CTTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCAT 38 0.95 No Hit GCTTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCA 36 0.8999999999999999 No Hit GGCCAAAATAACCATGAGCAGCCACAATATTATAAGTCTCTTCCTCTTGA 31 0.775 No Hit CTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCATTAAT 30 0.75 No Hit GGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTACGTT 30 0.75 No Hit GACCCATATAACAAGCTACACCAAGTAAGAAGTGTAGAACAATTAGCTCA 21 0.525 No Hit CTCATAAGGACCACCATTGTATAACCATTCATCAACGGATGCAGCTTCCC 20 0.5 No Hit GTTGATGATATCAGCCCAAGTATTAATAACGCGACCTTGACTATCAACTA 19 0.475 No Hit CGGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTACGT 18 0.44999999999999996 No Hit GTTAGCGGAAAAACCTTATCCATTAATAGCGGGAACTTCAAGAGCAGCTA 18 0.44999999999999996 No Hit GTTGAAACTAGCATATTGGAAGATTAATCGGCCAAAATAACCATGAGCAG 18 0.44999999999999996 No Hit GCTCTGCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTCCTAAA 17 0.42500000000000004 No Hit CCCAAGTATTAATAACGCGACCTTGACTATCAACTACAGATTGGTTGAAA 16 0.4 No Hit GTTGAAATTGAATCCATTTAGGTTGAACGCCATAGTACTAATACCTAAAG 16 0.4 No Hit GTGAGCATTACGTTCGTGCATTACTTCCATACCAAGATTAGCACGGTTGA 15 0.375 No Hit GCCAAAATAACCATGAGCAGCCACAATATTATAAGTCTCTTCCTCTTGAC 15 0.375 No Hit CCACAATATTATAAGTCTCTTCCTCTTGACCAAATTTGTAACCCTCATTA 14 0.35000000000000003 No Hit GTTTCCATAAAGTAAAGAACCAGAAACAGGCTCGCGAATACCATCAATAT 14 0.35000000000000003 No Hit CCAGATTCCTACTACAGGCCAAGCAGCCAAGAAGAAGTGTAAAGAACGAG 13 0.325 No Hit CATATAACAAGCTACACCAAGTAAGAAGTGTAGAACAATTAGCTCATAAG 13 0.325 No Hit GTGCTCTGCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTCCTA 13 0.325 No Hit GTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTC 12 0.3 No Hit GGGAAGTTGTGAGCATTACGTTCGTGCATTACTTCCATACCAAGATTAGC 12 0.3 No Hit CCACCATTGTATAACCATTCATCAACGGATGCAGCTTCCCAAATTGGGTA 12 0.3 No Hit GGATGCATAAGGATGTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAA 12 0.3 No Hit GCATAAGGATGTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAAAGTA 12 0.3 No Hit GCCAAGAAGAAGTGTAAAGAACGAGAGTTGTTGAAACTAGCATATTGGAA 12 0.3 No Hit GTTGCGGTCAATAAGGTAGGGATCATCAAAACACCGAACCATCCGATGTA 11 0.27499999999999997 No Hit TTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCATTA 11 0.27499999999999997 No Hit CCCTCATTAGCAGATTCATTTTCAGTAGTTTCCCTGATCAAACTAGAGGT 11 0.27499999999999997 No Hit GGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTACGTTC 11 0.27499999999999997 No Hit CGAGAGTTGTTGAAACTAGCATATTGGAAGATTAATCGGCCAAAATAACC 10 0.25 No Hit CAGTGAACCAGATTCCTACTACAGGCCAAGCAGCCAAGAAGAAGTGTAAA 10 0.25 No Hit GTCGCAGCTGCAACAGGAGCTGAATATGCAACAGCAATCCAAGGGCGCAT 10 0.25 No Hit GCGACCTTGACTATCAACTACAGATTGGTTGAAATTGAATCCATTTAGGT 10 0.25 No Hit CGCAGAAGTAGGAATAATAGCACCAGAGATAATATTGTTTCCATAAAGTA 9 0.22499999999999998 No Hit GCCGAAAACACCAGCTACACCTAACATGTGAAATGGATGCATAAGGATGT 9 0.22499999999999998 No Hit GGTTGATGATATCAGCCCAAGTATTAATAACGCGACCTTGACTATCAACT 9 0.22499999999999998 No Hit GGTCAATAAGGTAGGGATCATCAAAACACCGAACCATCCGATGTAAAGAC 9 0.22499999999999998 No Hit CTTATATGTTAGCGGAAAAACCTTATCCATTAATAGCGGGAACTTCAAGA 9 0.22499999999999998 No Hit GATGCATAAGGATGTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAAA 9 0.22499999999999998 No Hit CTCTTGACCAAATTTGTAACCCTCATTAGCAGATTCATTTTCAGTAGTTT 9 0.22499999999999998 No Hit GCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTCCTAAAGGCAT 9 0.22499999999999998 No Hit CTCTGCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTCCTAAAG 9 0.22499999999999998 No Hit GGCGCATACCCAAACGGAAACTAAGTTCCCACTCACGACCCATATAACAA 8 0.2 No Hit GGCGATAATAAATACAGAAGTTGCGGTCAATAAGGTAGGGATCATCAAAA 8 0.2 No Hit TTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCATT 8 0.2 No Hit GCTAGTTATCCAGTTGCAGAAGCGACCCCACAGGCTTGTACTTTCGCGTC 8 0.2 No Hit GCTGCGATGAAGGCGATAATAAATACAGAAGTTGCGGTCAATAAGGTAGG 8 0.2 No Hit CGGAAAAACCTTATCCATTAATAGCGGGAACTTCAAGAGCAGCTAGATCT 8 0.2 No Hit GGCCAAGCAGCCAAGAAGAAGTGTAAAGAACGAGAGTTGTTGAAACTAGC 8 0.2 No Hit CATAAGGATGTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAAAGTAC 8 0.2 No Hit CATCAATATCTACTGGAGGGGCTGCGATGAAGGCGATAATAAATACAGAA 8 0.2 No Hit GTCCATGTACCAGTAGAAGATTCGGCAGCTACTGCAGCCCCTGCTTCTTC 8 0.2 No Hit GCGGAAAAACCTTATCCATTAATAGCGGGAACTTCAAGAGCAGCTAGATC 8 0.2 No Hit CGCGACCTTGACTATCAACTACAGATTGGTTGAAATTGAATCCATTTAGG 8 0.2 No Hit GCACGGTTGATGATATCAGCCCAAGTATTAATAACGCGACCTTGACTATC 7 0.17500000000000002 No Hit GGAGGGGCTGCGATGAAGGCGATAATAAATACAGAAGTTGCGGTCAATAA 7 0.17500000000000002 No Hit GGAAAAACCTTATCCATTAATAGCGGGAACTTCAAGAGCAGCTAGATCTA 7 0.17500000000000002 No Hit GGGAATTCGTAGATCCTCCAGACGTAGAGCACGTAGGGCTTTGAAACCAA 7 0.17500000000000002 No Hit AGCGGAAAAACCTTATCCATTAATAGCGGGAACTTCAAGAGCAGCTAGAT 7 0.17500000000000002 No Hit CTCGCGAATACCATCAATATCTACTGGAGGGGCTGCGATGAAGGCGATAA 7 0.17500000000000002 No Hit CCCATATAACAAGCTACACCAAGTAAGAAGTGTAGAACAATTAGCTCATA 6 0.15 No Hit GGTTGAAATTGAATCCATTTAGGTTGAACGCCATAGTACTAATACCTAAA 6 0.15 No Hit CTGCGATGAAGGCGATAATAAATACAGAAGTTGCGGTCAATAAGGTAGGG 6 0.15 No Hit GGCGGAAAAACCTTATCCATTAATAGCGGGAACTTCAAGAGCAGCTAGAT 6 0.15 No Hit GCTGAATATGCAACAGCAATCCAAGGGCGCATACCCAAACGGAAACTAAG 6 0.15 No Hit TGATGATATCAGCCCAAGTATTAATAACGCGACCTTGACTATCAACTACA 6 0.15 No Hit GTCAATTAGAAGAATAAAGAAGAATTACTGCATTTCGTAACTTATTTTTT 6 0.15 No Hit TTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCATTAATA 6 0.15 No Hit CATCAGAGAAGCTTCCTTGACCAATAGGGTAAATCAAGAAAACAGCAGTC 6 0.15 No Hit GGCTGCGATGAAGGCGATAATAAATACAGAAGTTGCGGTCAATAAGGTAG 6 0.15 No Hit CCACTCACGACCCATATAACAAGCTACACCAAGTAAGAAGTGTAGAACAA 6 0.15 No Hit ATCAATATCTACTGGAGGGGCTGCGATGAAGGCGATAATAAATACAGAAG 6 0.15 No Hit CATTAATAGCGGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGA 6 0.15 No Hit GTTCGTGCATTACTTCCATACCAAGATTAGCACGGTTGATGATATCAGCC 6 0.15 No Hit CACCATTGTATAACCATTCATCAACGGATGCAGCTTCCCAAATTGGGTAA 6 0.15 No Hit GGTAAATCAAGAAAACAGCAGTCGCAGCTGCAACAGGAGCTGAATATGCA 6 0.15 No Hit CCTCATTAGCAGATTCATTTTCAGTAGTTTCCCTGATCAAACTAGAGGTT 6 0.15 No Hit CCCGAAGTTACGGGGCTATTTTGCCGAGTTCCTTAGAGAGAGTTGTCTCG 5 0.125 No Hit CGCCATAGTACTAATACCTAAAGCAGTGAACCAGATTCCTACTACAGGCC 5 0.125 No Hit CTTCTAACTTACCTACTACTGTACCGGCGTGGATATGATCTCCCCCAGAC 5 0.125 No Hit CTTGACTATCAACTACAGATTGGTTGAAATTGAATCCATTTAGGTTGAAC 5 0.125 No Hit ACCAGATATTCCTAAAGGCATACCATCAGAGAAGCTTCCTTGACCAATAG 5 0.125 No Hit ACGAGAGTTGTTGAAACTAGCATATTGGAAGATTAATCGGCCAAAATAAC 5 0.125 No Hit GGGTAATGTTGCTCCAATACCTAACCAAAGAGCTACTGCAGTACCGATTA 5 0.125 No Hit CAAGTATTAATAACGCGACCTTGACTATCAACTACAGATTGGTTGAAATT 5 0.125 No Hit GCATTACGTTCGTGCATTACTTCCATACCAAGATTAGCACGGTTGATGAT 5 0.125 No Hit GGGCTGCGATGAAGGCGATAATAAATACAGAAGTTGCGGTCAATAAGGTA 5 0.125 No Hit GTAGGGATCATCAAAACACCGAACCATCCGATGTAAAGACGATTTTCAGT 5 0.125 No Hit GTGGTTCGCTGGAGTAGGCTGCGAGGCCCAAGCGGCCAAGGAGTGCAGCG 5 0.125 No Hit GTGCTAGTTATCCAGTTGCAGAAGCGACCCCACAGGCTTGTACTTTCGCG 5 0.125 No Hit GAGAGTTGTTGAAACTAGCATATTGGAAGATTAATCGGCCAAAATAACCA 5 0.125 No Hit GTCGTGAATAGCTCCGTGGAATAAAATAGAATTTCCTTATGCATAGAACT 5 0.125 No Hit CCCAGGAACAGGCTCGATGTGATAGCATCGTCCTTTGTAACGATCAAGAC 5 0.125 No Hit GAGCATTACGTTCGTGCATTACTTCCATACCAAGATTAGCACGGTTGATG 5 0.125 No Hit ATAACAAGCTACACCAAGTAAGAAGTGTAGAACAATTAGCTCATAAGGAC 5 0.125 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.025 0.0 0.0 0.0 0.0 20-21 0.025 0.0 0.0 0.0 0.0 22-23 0.025 0.0 0.0 0.0 0.0 24-25 0.025 0.0 0.0 0.0 0.0 26-27 0.025 0.0 0.0 0.0 0.0 28-29 0.025 0.0 0.0 0.0 0.0 30-31 0.025 0.0 0.0 0.0 0.0 32-33 0.025 0.0 0.0 0.0 0.0 34-35 0.025 0.0 0.0 0.0 0.0 36-37 0.025 0.0 0.0 0.0 0.0 38-39 0.025 0.0 0.0 0.0 0.0 40-41 0.025 0.0 0.0 0.0 0.0 42-43 0.025 0.0 0.0 0.0 0.0 44-45 0.025 0.0 0.0 0.0 0.0 46-47 0.025 0.0 0.0 0.0 0.0 48-49 0.025 0.0 0.0 0.0 0.0 50-51 0.025 0.0 0.0 0.0 0.0 52-53 0.025 0.0 0.0 0.0 0.0 54-55 0.025 0.0 0.0 0.0 0.0 56-57 0.025 0.0 0.0 0.0 0.0 58-59 0.025 0.0 0.0 0.0 0.0 60-61 0.025 0.0 0.0 0.0 0.0 62-63 0.037500000000000006 0.0 0.0 0.0 0.0 64-65 0.05 0.0 0.0 0.0 0.0 66-67 0.05 0.0 0.0 0.0 0.0 68-69 0.05 0.0 0.0 0.0 0.0 70-71 0.075 0.0 0.0 0.0 0.0 72-73 0.075 0.0 0.0 0.0 0.0 74-75 0.1 0.0 0.0 0.0 0.0 76-77 0.15 0.0 0.0 0.0 0.0 78-79 0.175 0.0 0.0 0.0 0.0 80-81 0.225 0.0 0.0 0.0 0.0 82-83 0.25 0.0 0.0 0.0 0.0 84-85 0.3125 0.0 0.0 0.0 0.0 86-87 0.4 0.0 0.0 0.0 0.0 88-89 0.4625 0.0 0.0 0.0 0.0 90-91 0.5375 0.0 0.0 0.0 0.0 92-93 0.6 0.0 0.0 0.0 0.0 94-95 0.6875 0.0 0.0 0.0 0.0 96-97 0.8 0.0 0.0 0.0 0.0 98-99 0.8875 0.0 0.0 0.0 0.0 100-101 1.1 0.0 0.0 0.0 0.0 102-103 1.35 0.0 0.0 0.0 0.0 104-105 1.7125 0.0 0.0 0.0 0.0 106-107 1.9625 0.0 0.0 0.0 0.0 108-109 2.2874999999999996 0.0 0.0 0.0 0.0 110-111 2.5250000000000004 0.0 0.0 0.0 0.0 112-113 2.8875 0.0 0.0 0.0 0.0 114-115 3.1375 0.0 0.0 0.0 0.0 116-117 3.4125 0.0 0.0 0.0 0.0 118-119 3.8375 0.0 0.0 0.0 0.0 120-121 4.075 0.0 0.0 0.0 0.0 122-123 4.387499999999999 0.0 0.0 0.0 0.0 124-125 4.9125 0.0 0.0 0.0 0.0 126-127 5.300000000000001 0.0 0.0 0.0 0.0 128-129 5.612500000000001 0.0 0.0 0.0 0.0 130-131 5.9375 0.0 0.0 0.0 0.0 132-133 6.225 0.0 0.0 0.0 0.0 134-135 6.75 0.0 0.0 0.0 0.0 136-137 7.137499999999999 0.0 0.0 0.0 0.0 138-139 7.5375 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content pass >>END_MODULE SRR6941551 read2 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR6941551_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 33.91675 35.0 35.0 35.0 33.0 35.0 2 34.10975 35.0 35.0 35.0 33.0 35.0 3 34.349 35.0 35.0 35.0 33.0 35.0 4 34.25675 35.0 35.0 35.0 33.0 35.0 5 34.351 35.0 35.0 35.0 33.0 35.0 6 38.932 40.0 40.0 40.0 38.0 40.0 7 38.9185 40.0 40.0 40.0 38.0 40.0 8 39.098 40.0 40.0 40.0 39.0 40.0 9 39.12125 40.0 40.0 40.0 39.0 40.0 10-14 39.0452 40.0 40.0 40.0 38.2 40.0 15-19 39.131099999999996 40.0 40.0 40.0 39.0 40.0 20-24 39.1356 40.0 40.0 40.0 39.0 40.0 25-29 39.092349999999996 40.0 40.0 40.0 38.6 40.0 30-34 39.0548 40.0 40.0 40.0 38.6 40.0 35-39 38.90475 40.0 39.8 40.0 38.0 40.0 40-44 38.947950000000006 40.0 40.0 40.0 38.4 40.0 45-49 38.92235 40.0 39.8 40.0 37.8 40.0 50-54 38.75945 40.0 39.0 40.0 37.2 40.0 55-59 38.734449999999995 40.0 39.2 40.0 37.0 40.0 60-64 38.6576 40.0 39.0 40.0 36.4 40.0 65-69 38.71825 40.0 39.0 40.0 37.4 40.0 70-74 38.5566 40.0 39.0 40.0 36.4 40.0 75-79 38.54905 40.0 39.0 40.0 36.6 40.0 80-84 38.587250000000004 40.0 39.0 40.0 36.8 40.0 85-89 38.73344999999999 40.0 39.0 40.0 37.0 40.0 90-94 38.6565 40.0 39.0 40.0 36.8 40.0 95-99 38.4144 40.0 39.0 40.0 36.0 40.0 100-104 37.13585 38.6 37.6 39.2 33.4 39.4 105-109 38.462149999999994 40.0 39.0 40.0 36.2 40.0 110-114 38.5065 40.0 39.0 40.0 36.6 40.0 115-119 38.3351 40.0 39.0 40.0 35.8 40.0 120-124 38.25435 40.0 39.0 40.0 35.6 40.0 125-129 38.35039999999999 40.0 39.0 40.0 36.2 40.0 130-134 38.250600000000006 40.0 39.0 40.0 36.0 40.0 135-139 38.16029999999999 40.0 39.0 40.0 35.8 40.0 140-144 37.8898 40.0 39.0 40.0 35.0 40.0 145-149 37.5017 40.0 38.8 40.0 33.8 40.0 150-151 34.59975 38.0 34.5 39.5 25.0 40.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 1.0 4 0.0 5 0.0 6 2.0 7 0.0 8 0.0 9 1.0 10 0.0 11 1.0 12 0.0 13 0.0 14 3.0 15 1.0 16 1.0 17 1.0 18 3.0 19 1.0 20 3.0 21 3.0 22 10.0 23 6.0 24 15.0 25 10.0 26 15.0 27 17.0 28 22.0 29 23.0 30 17.0 31 43.0 32 38.0 33 51.0 34 61.0 35 73.0 36 111.0 37 172.0 38 401.0 39 2891.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.299949418310575 21.421345472938796 12.44309559939302 24.83560950935761 2 23.777276147479306 23.727113117632307 36.11738148984199 16.3782292450464 3 18.825 24.725 39.0 17.45 4 22.8 31.85 26.775 18.575 5 24.75 31.4 27.474999999999998 16.375 6 20.175 34.8 28.375 16.650000000000002 7 17.2 21.5 43.75 17.549999999999997 8 19.025 24.075 32.95 23.95 9 22.275 20.05 35.9 21.775 10-14 22.545 27.045 31.915 18.495 15-19 22.6 25.790000000000003 31.765 19.845 20-24 22.2 25.795 32.695 19.31 25-29 22.685 25.869999999999997 32.095 19.35 30-34 23.29 25.319999999999997 32.12 19.27 35-39 24.23 24.675 31.485000000000003 19.61 40-44 23.080000000000002 26.179999999999996 31.465 19.275000000000002 45-49 21.97 26.87 31.480000000000004 19.68 50-54 22.025 26.6 31.59 19.785 55-59 21.695 26.625 31.09 20.59 60-64 22.314999999999998 26.085 31.6 20.0 65-69 22.5 26.105 30.98 20.415 70-74 22.575 26.179999999999996 30.995 20.25 75-79 23.175 25.44 31.045 20.34 80-84 22.66 25.05 33.305 18.985 85-89 23.544999999999998 25.77 31.105 19.580000000000002 90-94 23.04 26.095000000000002 30.570000000000004 20.294999999999998 95-99 22.88 26.145000000000003 30.985000000000003 19.99 100-104 22.59 26.27 31.15 19.99 105-109 24.165 24.965 31.205 19.665 110-114 23.47 25.369999999999997 31.465 19.695 115-119 22.99 25.465 31.069999999999997 20.474999999999998 120-124 23.155 25.56 30.78 20.505000000000003 125-129 24.395 25.8 29.475 20.330000000000002 130-134 22.88 26.474999999999998 30.409999999999997 20.235 135-139 23.080000000000002 26.05 31.119999999999997 19.75 140-144 24.349999999999998 26.135 30.509999999999998 19.005 145-149 23.974999999999998 25.540000000000003 30.599999999999998 19.885 150-151 23.086558937742705 25.203557559814605 32.30615056996117 19.403732932481525 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.5 10 1.0 11 0.5 12 0.0 13 0.0 14 0.5 15 0.5 16 0.0 17 0.0 18 0.0 19 2.0 20 2.5 21 2.0 22 3.5 23 4.0 24 6.0 25 11.0 26 14.0 27 11.5 28 16.0 29 27.0 30 32.5 31 40.5 32 54.5 33 66.5 34 85.0 35 106.5 36 127.0 37 191.0 38 236.5 39 229.0 40 264.5 41 284.0 42 250.0 43 248.5 44 263.5 45 239.5 46 208.5 47 162.5 48 119.0 49 89.0 50 64.5 51 65.5 52 49.5 53 44.0 54 54.0 55 49.0 56 36.0 57 25.0 58 28.5 59 34.5 60 30.5 61 27.0 62 24.0 63 17.5 64 10.0 65 5.5 66 6.5 67 8.5 68 6.0 69 3.5 70 3.0 71 2.0 72 1.5 73 0.5 74 0.0 75 0.5 76 0.5 77 0.0 78 1.0 79 1.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 1.15 2 0.325 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.21250000000000002 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 66.8 #Duplication Level Percentage of deduplicated Percentage of total 1 82.37275449101796 55.025 2 8.607784431137725 11.5 3 2.9565868263473054 5.925 4 2.020958083832335 5.4 5 1.0479041916167664 3.5000000000000004 6 1.0853293413173652 4.35 7 0.4491017964071856 2.1 8 0.2619760479041916 1.4000000000000001 9 0.18712574850299402 1.125 >10 1.0104790419161676 9.675 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source ATTATCTATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAAT 35 0.8750000000000001 No Hit TATCTATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTT 29 0.7250000000000001 No Hit ATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAA 20 0.5 No Hit GTGGGAACTTAGTTTCCGTTTGGGTATGCGCCCTTGGATTGCTGTTGCAT 19 0.475 No Hit GTTATTTTGGCCGATTAATCTTCCAATATGCTAGTTTCAACAACTCTCGT 17 0.42500000000000004 No Hit GCTAGTTTCAACAACTCTCGTTCTTTACACTTCTTCTTGGCTGCTTGGCC 16 0.4 No Hit GCTTGTTATATGGGTCGTGAGTGGGAACTTAGTTTCCGTTTGGGTATGCG 16 0.4 No Hit GGAAGCTTCTCTGATGGTATGCCTTTAGGAATATCTGGTACTTTCAACTT 16 0.4 No Hit GCACAACATCCTTATGCATCCATTTCACATGTTAGGTGTAGCTGGTGTTT 16 0.4 No Hit CTATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAA 14 0.35000000000000003 No Hit GTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGATCTTACCATGAC 14 0.35000000000000003 No Hit ATCTATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTG 14 0.35000000000000003 No Hit GCATGGTTCCTTGGTAACCTCTAGTTTGATCAGGGAAACTACTGAAAATG 13 0.325 No Hit CTGGTACTTTCAACTTTATGATTGTATTCCAGGCAGAGCACAACATCCTT 12 0.3 No Hit GTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGATCTT 11 0.27499999999999997 No Hit CTAGCACTGAAAATCGTCTTTACATCGGATGGTTCGGTGTTTTGATGATC 11 0.27499999999999997 No Hit GTAGGAATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCT 11 0.27499999999999997 No Hit AGGGAAACTACTGAAAATGAATCTGCTAATGAGGGTTACAAATTTGGTCA 11 0.27499999999999997 No Hit GTTAGGTGTAGCTGGTGTTTTCGGCGGTTCCCTATTCAGTGCTATGCATG 11 0.27499999999999997 No Hit CAACATCCTTATGCATCCATTTCACATGTTAGGTGTAGCTGGTGTTTTCG 11 0.27499999999999997 No Hit AGCACAACATCCTTATGCATCCATTTCACATGTTAGGTGTAGCTGGTGTT 10 0.25 No Hit GTCGTGAGTGGGAACTTAGTTTCCGTTTGGGTATGCGCCCTTGGATTGCT 10 0.25 No Hit CAAATTTGGTCAAGAGGAAGAGACTTATAATATTGTGGCTGCTCATGGTT 10 0.25 No Hit GCTGCGACTGCTGTTTTCTTGATTTACCCTATTGGTCAAGGAAGCTTCTC 10 0.25 No Hit TCTATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGA 10 0.25 No Hit GTTCAACCTAAATGGATTCAATTTCAACCAATCTGTAGTTGATAGTCAAG 10 0.25 No Hit CTGAAAATGAATCTGCTAATGAGGGTTACAAATTTGGTCAAGAGGAAGAG 10 0.25 No Hit ATTCAGTGCTATGCATGGTTCCTTGGTAACCTCTAGTTTGATCAGGGAAA 9 0.22499999999999998 No Hit GGGAACTTAGTTTCCGTTTGGGTATGCGCCCTTGGATTGCTGTTGCATAT 9 0.22499999999999998 No Hit GTTTCAACAACTCTCGTTCTTTACACTTCTTCTTGGCTGCTTGGCCTGTA 9 0.22499999999999998 No Hit GTTATTGTGAGAATTCTTAATTCAAGAGTTGTAAGGAGGGACTTATGTCA 9 0.22499999999999998 No Hit CACATGTTAGGTGTAGCTGGTGTTTTCGGCGGTTCCCTATTCAGTGCTAT 9 0.22499999999999998 No Hit GGAAGAGACTTATAATATTGTGGCTGCTCATGGTTATTTTGGCCGATTAA 8 0.2 No Hit GGGAAACTACTGAAAATGAATCTGCTAATGAGGGTTACAAATTTGGTCAA 8 0.2 No Hit GAGACTTATAATATTGTGGCTGCTCATGGTTATTTTGGCCGATTAATCTT 8 0.2 No Hit CATCAACCGTGCTAATCTTGGTATGGAAGTAATGCACGAACGTAATGCTC 8 0.2 No Hit CAATGGTGGTCCTTATGAGCTAATTGTTCTACACTTCTTACTTGGTGTAG 8 0.2 No Hit CTTTATGATTGTATTCCAGGCAGAGCACAACATCCTTATGCATCCATTTC 8 0.2 No Hit GAATAAACCAAGATCTTACCATGACTGCAATTTTAGAGAGACGCGAAAGT 8 0.2 No Hit CCTACTTCTGCGGCAATCGGATTGCACTTTTACCCAATTTGGGAAGCTGC 7 0.17500000000000002 No Hit ATCGGATTGCACTTTTACCCAATTTGGGAAGCTGCATCCGTTGATGAATG 7 0.17500000000000002 No Hit GGAAACTACTGAAAATGAATCTGCTAATGAGGGTTACAAATTTGGTCAAG 7 0.17500000000000002 No Hit CAACTCTCGTTCTTTACACTTCTTCTTGGCTGCTTGGCCTGTAGTAGGAA 7 0.17500000000000002 No Hit GTTCCTTGGTAACCTCTAGTTTGATCAGGGAAACTACTGAAAATGAATCT 7 0.17500000000000002 No Hit GACTTATAATATTGTGGCTGCTCATGGTTATTTTGGCCGATTAATCTTCC 7 0.17500000000000002 No Hit CGCAGCCCCTCCAGTAGATATTGATGGTATTCGCGAGCCTGTTTCTGGTT 7 0.17500000000000002 No Hit CTTGCAATTTGAATAAACCAAGATCTTACCATGACTGCAATTTTAGAGAG 7 0.17500000000000002 No Hit AAACAATATTATCTCTGGTGCTATTATTCCTACTTCTGCGGCAATCGGAT 7 0.17500000000000002 No Hit ATTGTATTCCAGGCAGAGCACAACATCCTTATGCATCCATTTCACATGTT 7 0.17500000000000002 No Hit GAAGAGACTTATAATATTGTGGCTGCTCATGGTTATTTTGGCCGATTAAT 7 0.17500000000000002 No Hit GTTCTTTACTTTATGGAAACAATATTATCTCTGGTGCTATTATTCCTACT 7 0.17500000000000002 No Hit CTATTCAGTGCTATGCATGGTTCCTTGGTAACCTCTAGTTTGATCAGGGA 6 0.15 No Hit CGGCGGTTCCCTATTCAGTGCTATGCATGGTTCCTTGGTAACCTCTAGTT 6 0.15 No Hit GACCTTGTTATTGTGAGAATTCTTAATTCAAGAGTTGTAAGGAGGGACTT 6 0.15 No Hit CCTTGTTATTGTGAGAATTCTTAATTCAAGAGTTGTAAGGAGGGACTTAT 6 0.15 No Hit CATGGTTATTTTGGCCGATTAATCTTCCAATATGCTAGTTTCAACAACTC 6 0.15 No Hit ATTATTCCTACTTCTGCGGCAATCGGATTGCACTTTTACCCAATTTGGGA 6 0.15 No Hit CTTTTACCCAATTTGGGAAGCTGCATCCGTTGATGAATGGTTATACAATG 6 0.15 No Hit GTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCTAAATGGATTCA 6 0.15 No Hit CCTAAATGGATTCAATTTCAACCAATCTGTAGTTGATAGTCAAGGTCGCG 6 0.15 No Hit GCAATTTTAGAGAGACGCGAAAGTACAAGCCTGTGGGGTCGCTTCTGCAA 6 0.15 No Hit TATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAAT 6 0.15 No Hit CGTTGATGAATGGTTATACAATGGTGGTCCTTATGAGCTAATTGTTCTAC 6 0.15 No Hit GTTTTGATGATCCCTACCTTATTGACCGCAACTTCTGTATTTATTATCGC 6 0.15 No Hit GGAATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCTAAA 6 0.15 No Hit GTAGCTTGTTATATGGGTCGTGAGTGGGAACTTAGTTTCCGTTTGGGTAT 6 0.15 No Hit CGCGAAATCACTTTAGGTTTTGTTGATTTATTGCGCGACGATTTTATTGA 6 0.15 No Hit ATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCA 6 0.15 No Hit GTTGCATATTCAGCTCCTGTTGCAGCTGCGACTGCTGTTTTCTTGATTTA 6 0.15 No Hit GCCTTTAGGAATATCTGGTACTTTCAACTTTATGATTGTATTCCAGGCAG 6 0.15 No Hit GTAACCTCTAGTTTGATCAGGGAAACTACTGAAAATGAATCTGCTAATGA 6 0.15 No Hit GAGGAAGAGACTTATAATATTGTGGCTGCTCATGGTTATTTTGGCCGATT 6 0.15 No Hit CGAAAGTACAAGCCTGTGGGGTCGCTTCTGCAACTGGATAACTAGCACTG 6 0.15 No Hit CTTGTTATTGTGAGAATTCTTAATTCAAGAGTTGTAAGGAGGGACTTATG 6 0.15 No Hit CGGCAATCGGATTGCACTTTTACCCAATTTGGGAAGCTGCATCCGTTGAT 6 0.15 No Hit GTACAAGCCTGTGGGGTCGCTTCTGCAACTGGATAACTAGCACTGAAAAT 6 0.15 No Hit GTTTGATCAGGGAAACTACTGAAAATGAATCTGCTAATGAGGGTTACAAA 6 0.15 No Hit CTTCCAATATGCTAGTTTCAACAACTCTCGTTCTTTACACTTCTTCTTGG 6 0.15 No Hit GAATGGTTATACAATGGTGGTCCTTATGAGCTAATTGTTCTACACTTCTT 6 0.15 No Hit GGTACTTTCAACTTTATGATTGTATTCCAGGCAGAGCACAACATCCTTAT 6 0.15 No Hit GCTCATGGTTATTTTGGCCGATTAATCTTCCAATATGCTAGTTTCAACAA 5 0.125 No Hit GCTAATTGTTCTACACTTCTTACTTGGTGTAGCTTGTTATATGGGTCGTG 5 0.125 No Hit CTGCAACTGGATAACTAGCACTGAAAATCGTCTTTACATCGGATGGTTCG 5 0.125 No Hit AAATTTGGTCAAGAGGAAGAGACTTATAATATTGTGGCTGCTCATGGTTA 5 0.125 No Hit GGGTTACAAATTTGGTCAAGAGGAAGAGACTTATAATATTGTGGCTGCTC 5 0.125 No Hit GGCTGCTTGGCCTGTAGTAGGAATCTGGTTCACTGCTTTAGGTATTAGTA 5 0.125 No Hit CCTGTAGTAGGAATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTT 5 0.125 No Hit GGCCTGTAGTAGGAATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCG 5 0.125 No Hit AGTTGATAGTCAAGGTCGCGTTATTAATACTTGGGCTGATATCATCAACC 5 0.125 No Hit ATTTGGTCAAGAGGAAGAGACTTATAATATTGTGGCTGCTCATGGTTATT 5 0.125 No Hit AGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGATCT 5 0.125 No Hit GTTATATGGGTCGTGAGTGGGAACTTAGTTTCCGTTTGGGTATGCGCCCT 5 0.125 No Hit CACAACATCCTTATGCATCCATTTCACATGTTAGGTGTAGCTGGTGTTTT 5 0.125 No Hit CTTGGTAACCTCTAGTTTGATCAGGGAAACTACTGAAAATGAATCTGCTA 5 0.125 No Hit ATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCTAAATGG 5 0.125 No Hit GTTGATAGTCAAGGTCGCGTTATTAATACTTGGGCTGATATCATCAACCG 5 0.125 No Hit GGATGGTTCGGTGTTTTGATGATCCCTACCTTATTGACCGCAACTTCTGT 5 0.125 No Hit TGGTTATTTTGGCCGATTAATCTTCCAATATGCTAGTTTCAACAACTCTC 5 0.125 No Hit CCCTATTCAGTGCTATGCATGGTTCCTTGGTAACCTCTAGTTTGATCAGG 5 0.125 No Hit GTTTCTGGTTCTTTACTTTATGGAAACAATATTATCTCTGGTGCTATTAT 5 0.125 No Hit GTTGATGAATGGTTATACAATGGTGGTCCTTATGAGCTAATTGTTCTACA 5 0.125 No Hit CGGATGGTTCGGTGTTTTGATGATCCCTACCTTATTGACCGCAACTTCTG 5 0.125 No Hit GAGAATTCTTAATTCAAGAGTTGTAAGGAGGGACTTATGTCACCACAAAC 5 0.125 No Hit GGTTCTTTACTTTATGGAAACAATATTATCTCTGGTGCTATTATTCCTAC 5 0.125 No Hit TGGTTATACAATGGTGGTCCTTATGAGCTAATTGTTCTACACTTCTTACT 5 0.125 No Hit GTAGATATTGATGGTATTCGCGAGCCTGTTTCTGGTTCTTTACTTTATGG 5 0.125 No Hit ATTGATGGTATTCGCGAGCCTGTTTCTGGTTCTTTACTTTATGGAAACAA 5 0.125 No Hit GTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGATCTTACCATGACTG 5 0.125 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.025 0.0 0.0 0.0 0.0 20-21 0.025 0.0 0.0 0.0 0.0 22-23 0.025 0.0 0.0 0.0 0.0 24-25 0.025 0.0 0.0 0.0 0.0 26-27 0.025 0.0 0.0 0.0 0.0 28-29 0.025 0.0 0.0 0.0 0.0 30-31 0.025 0.0 0.0 0.0 0.0 32-33 0.025 0.0 0.0 0.0 0.0 34-35 0.025 0.0 0.0 0.0 0.0 36-37 0.025 0.0 0.0 0.0 0.0 38-39 0.025 0.0 0.0 0.0 0.0 40-41 0.025 0.0 0.0 0.0 0.0 42-43 0.025 0.0 0.0 0.0 0.0 44-45 0.025 0.0 0.0 0.0 0.0 46-47 0.025 0.0 0.0 0.0 0.0 48-49 0.025 0.0 0.0 0.0 0.0 50-51 0.025 0.0 0.0 0.0 0.0 52-53 0.025 0.0 0.0 0.0 0.0 54-55 0.025 0.0 0.0 0.0 0.0 56-57 0.025 0.0 0.0 0.0 0.0 58-59 0.025 0.0 0.0 0.0 0.0 60-61 0.025 0.0 0.0 0.0 0.0 62-63 0.037500000000000006 0.0 0.0 0.0 0.0 64-65 0.05 0.0 0.0 0.0 0.0 66-67 0.05 0.0 0.0 0.0 0.0 68-69 0.05 0.0 0.0 0.0 0.0 70-71 0.075 0.0 0.0 0.0 0.0 72-73 0.075 0.0 0.0 0.0 0.0 74-75 0.1 0.0 0.0 0.0 0.0 76-77 0.15 0.0 0.0 0.0 0.0 78-79 0.175 0.0 0.0 0.0 0.0 80-81 0.225 0.0 0.0 0.0 0.0 82-83 0.25 0.0 0.0 0.0 0.0 84-85 0.3125 0.0 0.0 0.0 0.0 86-87 0.4 0.0 0.0 0.0 0.0 88-89 0.4625 0.0 0.0 0.0 0.0 90-91 0.5375 0.0 0.0 0.0 0.0 92-93 0.6 0.0 0.0 0.0 0.0 94-95 0.6875 0.0 0.0 0.0 0.0 96-97 0.7875 0.0 0.0 0.0 0.0 98-99 0.8625 0.0 0.0 0.0 0.0 100-101 1.075 0.0 0.0 0.0 0.0 102-103 1.3 0.0 0.0 0.0 0.0 104-105 1.6625 0.0 0.0 0.0 0.0 106-107 1.9375 0.0 0.0 0.0 0.0 108-109 2.2625 0.0 0.0 0.0 0.0 110-111 2.5 0.0 0.0 0.0 0.0 112-113 2.8625 0.0 0.0 0.0 0.0 114-115 3.1125 0.0 0.0 0.0 0.0 116-117 3.3875 0.0 0.0 0.0 0.0 118-119 3.8125 0.0 0.0 0.0 0.0 120-121 4.025 0.0 0.0 0.0 0.0 122-123 4.3375 0.0 0.0 0.0 0.0 124-125 4.8625 0.0 0.0 0.0 0.0 126-127 5.25 0.0 0.0 0.0 0.0 128-129 5.5625 0.0 0.0 0.0 0.0 130-131 5.9 0.0 0.0 0.0 0.0 132-133 6.2 0.0 0.0 0.0 0.0 134-135 6.725 0.0 0.0 0.0 0.0 136-137 7.112500000000001 0.0 0.0 0.0 0.0 138-139 7.5125 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content pass >>END_MODULE Read 1132062 spots for SRR6941551.sra Written 1132062 spots for SRR6941551.sra Read 1132062 spots for SRR6941551.sra Written 1132062 spots for SRR6941551.sra Read 1132062 spots for SRR6941551.sra Written 1132062 spots for SRR6941551.sra Read 1132062 spots for SRR6941551.sra Written 1132062 spots for SRR6941551.sra Read 1132062 spots for SRR6941551.sra Written 1132062 spots for SRR6941551.sra Read 1132062 spots for SRR6941551.sra Written 1132062 spots for SRR6941551.sra Read 1132062 spots for SRR6941551.sra Written 1132062 spots for SRR6941551.sra Read 1132062 spots for SRR6941551.sra Written 1132062 spots for SRR6941551.sra Read 1132062 spots for SRR6941551.sra Written 1132062 spots for SRR6941551.sra Read 1132062 spots for SRR6941551.sra Written 1132062 spots for SRR6941551.sra Read 1132062 spots for SRR6941551.sra Written 1132062 spots for SRR6941551.sra Read 1132072 spots for SRR6941551.sra Written 1132072 spots for SRR6941551.sra Read 1132062 spots for SRR6941551.sra Written 1132062 spots for SRR6941551.sra Read 1132062 spots for SRR6941551.sra Written 1132062 spots for SRR6941551.sra Read 1132062 spots for SRR6941551.sra Written 1132062 spots for SRR6941551.sra Read 1132062 spots for SRR6941551.sra Written 1132062 spots for SRR6941551.sra Read 1132062 spots for SRR6941551.sra Written 1132062 spots for SRR6941551.sra Read 1132062 spots for SRR6941551.sra Written 1132062 spots for SRR6941551.sra Read 1132062 spots for SRR6941551.sra Written 1132062 spots for SRR6941551.sra Read 1132062 spots for SRR6941551.sra Written 1132062 spots for SRR6941551.sra SRR ids: ['SRR6941551.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_c4yq22lb SRR6941551.sra spots: 22641250 blocks: [[1, 1132062], [1132063, 2264124], [2264125, 3396186], [3396187, 4528248], [4528249, 5660310], [5660311, 6792372], [6792373, 7924434], [7924435, 9056496], [9056497, 10188558], [10188559, 11320620], [11320621, 12452682], [12452683, 13584744], [13584745, 14716806], [14716807, 15848868], [15848869, 16980930], [16980931, 18112992], [18112993, 19245054], [19245055, 20377116], [20377117, 21509178], [21509179, 22641250]] SRR6941551 file size 7650676 SRR6941551 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR6941551 SRR6941551_1.fastq SRR6941551_2.fastq Input file: SRR6941551_1.fastq Paired file: SRR6941551_2.fastq trimmed: SRR6941551-trimmed-pair1.fastq, SRR6941551-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Fri Dec 6 11:03:41 2024 >> started Fri Dec 6 11:04:09 2024 >> done (27.713s) 22641250 read pairs processed; of these: 6152 ( 0.03%) short read pairs filtered out after trimming by size control 8362 ( 0.04%) empty read pairs filtered out after trimming by size control 22626736 (99.94%) read pairs available; of these: 3964784 (17.52%) trimmed read pairs available after processing 18661952 (82.48%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 2 0.00% 19 0 0.00% 20 1 0.00% 21 2 0.00% 22 3 0.00% 23 2 0.00% 24 2 0.00% 25 7 0.00% 26 4 0.00% 27 4 0.00% 28 4 0.00% 29 4 0.00% 30 9 0.00% 31 3 0.00% 32 16 0.00% 33 18 0.00% 34 18 0.00% 35 22 0.00% 36 24 0.00% 37 27 0.00% 38 21 0.00% 39 44 0.00% 40 57 0.00% 41 62 0.00% 42 64 0.00% 43 93 0.00% 44 81 0.00% 45 88 0.00% 46 89 0.00% 47 101 0.00% 48 129 0.00% 49 160 0.00% 50 209 0.00% 51 202 0.00% 52 257 0.00% 53 305 0.00% 54 344 0.00% 55 367 0.00% 56 431 0.00% 57 422 0.00% 58 530 0.00% 59 555 0.00% 60 660 0.00% 61 746 0.00% 62 1070 0.00% 63 1165 0.01% 64 1336 0.01% 65 1390 0.01% 66 1550 0.01% 67 1744 0.01% 68 1895 0.01% 69 2260 0.01% 70 2593 0.01% 71 3145 0.01% 72 3575 0.02% 73 3812 0.02% 74 3960 0.02% 75 4798 0.02% 76 4565 0.02% 77 5380 0.02% 78 5386 0.02% 79 5833 0.03% 80 6686 0.03% 81 7625 0.03% 82 8576 0.04% 83 9080 0.04% 84 9621 0.04% 85 11510 0.05% 86 11919 0.05% 87 12480 0.06% 88 13922 0.06% 89 14331 0.06% 90 16031 0.07% 91 16231 0.07% 92 19015 0.08% 93 19141 0.08% 94 20604 0.09% 95 22233 0.10% 96 21252 0.09% 97 20794 0.09% 98 21016 0.09% 99 22390 0.10% 100 22758 0.10% 101 24722 0.11% 102 26837 0.12% 103 26778 0.12% 104 29099 0.13% 105 30530 0.13% 106 31140 0.14% 107 30872 0.14% 108 30634 0.14% 109 33431 0.15% 110 33146 0.15% 111 36612 0.16% 112 37860 0.17% 113 36187 0.16% 114 38776 0.17% 115 35733 0.16% 116 37766 0.17% 117 36402 0.16% 118 36508 0.16% 119 37109 0.16% 120 37961 0.17% 121 39238 0.17% 122 42940 0.19% 123 43934 0.19% 124 45021 0.20% 125 45801 0.20% 126 43862 0.19% 127 44394 0.20% 128 44747 0.20% 129 49969 0.22% 130 49165 0.22% 131 51936 0.23% 132 52570 0.23% 133 46307 0.20% 134 53250 0.24% 135 49983 0.22% 136 52820 0.23% 137 51167 0.23% 138 56740 0.25% 139 59621 0.26% 140 57432 0.25% 141 68772 0.30% 142 60376 0.27% 143 65428 0.29% 144 63853 0.28% 145 73024 0.32% 146 77966 0.34% 147 87248 0.39% 148 110062 0.49% 149 163875 0.72% 150 1156319 5.11% 151 18661952 82.48% 22626736 reads passed initial QC criterion=sequence-density sequence-density=0.31 sequence-density-rank=1 fanout-score=1.98 fanout-score-rank=31 prefix-density=0.30 prefix-fanout=2.0 sequence=TCTAATTCAAAA criterion=fanout-score sequence-density=0.01 sequence-density-rank=31 fanout-score=63.82 fanout-score-rank=1 prefix-density=0.36 prefix-fanout=2.4 sequence=AGAAAAAAACAAGTTTGCATCTTCAGGAGAATCTATATTTTCGCGAAATGGATCATAATAAAATGGATTTTAGGTATCTAGGGAAAATTCACTTCGAAGTAACTATTTCCTAGATACCTATGCACGGTACTTCACGGTTGAATGAATCAACCTGAAAAATACCTAAAAAAGGCCTAAAGTTAAGGATTTATCAATGGGTAATGTTGCTCCAATACCTAACCAAAGAGCTACTGCAGTACCGATTAAAAAAACGGTCGTAGCTACTGGGCGACGAAATGGATTTTGGAATTTGTTGACATTCTCTAGAAAAGGTACTGTCAATAAGCCTGTTGGCACAGAAACCATTAAGAGAACGCCCAATAACTTATTGGGTACCGTACGGAGTATTTGAAACACGGGAAAGAAGTACCACTCGGGTAATATTTCCAAAGGAGTTGCAAACGGATCCGCGGGTTCACCAATCATTGATGGCTCGAGAACAGCTAAACCTACATTACATGCAATAGTACCT criterion=sequence-density sequence-density=0.35 sequence-density-rank=1 fanout-score=5.86 fanout-score-rank=10 prefix-density=1.02 prefix-fanout=2.0 sequence=TGGGGAAGAGGA criterion=fanout-score sequence-density=0.01 sequence-density-rank=36 fanout-score=21.43 fanout-score-rank=1 prefix-density=0.05 prefix-fanout=2.5 sequence=TATTATAAACAGAATTAGCTTCTTTTTTTTAATGGAATGAAATAAATATTCACGCTTTCTGACACAGAATCCCATAGAAGGGTTAGGTCCATAGGATATGGATAGTCTTTTACAATGCGATAAAATAAAGCGACATCGTGTCTATTTTTCTTTGCTAAAGAGGTATTTCCATGGGTTTGCCTTGGTATCGTGTTCATACTGTCGTATTGAATGATCCGGGTCGATTGCTTGCGGTGCATATAATGCACACAGCTCTAGTTTCTGGTTGGGCTGGCTCGATGGCTTTATACGAATTAGCGGTTTTTGATCCCTCTGATCCTGTTCTGGATCCAATGTGGAGACAAGGTATGTTCGTCATTCCCTTCATGACTCGTTTAGGAATAACAGATTCGTGGGGTGGTTGGAGTATTTCAGGAGGAACTGTAACAAATCCGGGTATTTGGAGTTATGAAGGTGTGGCAGGTACGCATATTGTGTTTTCTGGCTTGTGTTTCTTGGCAGCTATCTGGCA SRR6941551 testing PE reads STAR mapping to Ensembl genome Started job on | Dec 06 11:05:27 Started mapping on | Dec 06 11:05:27 Finished on | Dec 06 11:07:28 Mapping speed, Million of reads per hour | 673.19 Number of input reads | 22626736 Average input read length | 295 UNIQUE READS: Uniquely mapped reads number | 13646980 Uniquely mapped reads % | 60.31% Average mapped length | 296.19 Number of splices: Total | 2801621 Number of splices: Annotated (sjdb) | 2511443 Number of splices: GT/AG | 2640060 Number of splices: GC/AG | 32275 Number of splices: AT/AC | 14609 Number of splices: Non-canonical | 114677 Mismatch rate per base, % | 0.26% Deletion rate per base | 0.02% Deletion average length | 1.97 Insertion rate per base | 0.02% Insertion average length | 2.69 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 7935999 % of reads mapped to multiple loci | 35.07% Number of reads mapped to too many loci | 13947 % of reads mapped to too many loci | 0.06% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 4.20% % of reads unmapped: other | 0.35% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 1049712 1049712 1049712 N_multimapping 7935999 7935999 7935999 N_noFeature 5254560 13044342 5512868 N_ambiguous 628813 9282 296120 UnstrandedReadsAssigned:7763607 PositiveStrandReadsAssigned:593356 NegativeStrandReadsAssigned:7837992 Dataset is classified negative stranded MeadianReadLen=151 20thPercentileLength=151 echo kmer=147 SRR6941551 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,972 [index] number of k-mers: 66,720,672 [index] number of equivalence classes: 111,837 [quant] running in paired-end mode [quant] will process pair 1: SRR6941551-trimmed-pair1.fastq SRR6941551-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 22,626,736 reads, 13,262,346 reads pseudoaligned [quant] estimated average fragment length: 246.736 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,046 rounds 52973 SRR6941551.ke.tsv 35125 SRR6941551.se.tsv 88098 total ==> SRR6941551.ke.tsv <== target_id length eff_length est_counts tpm PNS24245 936 690.811 0 0 PNS24247 1044 798.264 5.98897 0.577244 PNS24249 1928 1682.26 11.4876 0.525397 PNS24246 1044 798.264 5.98897 0.577244 PNS24248 1044 798.264 5.98897 0.577244 PNS24244 1471 1225.26 28.5455 1.79252 PNS24243 293 98.1807 0 0 KQK14069 1603 1357.26 363.779 20.6218 KQK14071 474 240.609 3.522 1.12624 ==> SRR6941551.se.tsv <== BRADI_1g14170v3 479 BRADI_1g53295v3 28 BRADI_1g59795v3 13 BRADI_1g07683v3 0 BRADI_1g00485v3 0 BRADI_1g20270v3 84 BRADI_1g74790v3 17 BRADI_1g09890v3 0 BRADI_1g77505v3 28 BRADI_1g48960v3 0 SRR6941551 completed mapping pipeline successfully