Starting /dee2/code/volunteer_pipeline.sh SRR6941552 current disk space = 1551568789504 free memory = 1601352068 SRR6941552 SRAfilesize 25595192aca2390fb74c9b4985d224d4 SRR6941552.sra SRR6941552.sra file validated SRR6941552 is paired end SRR6941552 is conventional basespace SRR6941552 read1 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR6941552_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 52 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 34.501 35.0 35.0 35.0 35.0 35.0 2 34.4635 35.0 35.0 35.0 33.0 35.0 3 34.59475 35.0 35.0 35.0 34.0 35.0 4 34.60175 35.0 35.0 35.0 35.0 35.0 5 34.43175 35.0 35.0 35.0 34.0 35.0 6 39.2745 40.0 40.0 40.0 39.0 40.0 7 39.24725 40.0 40.0 40.0 39.0 40.0 8 39.1565 40.0 40.0 40.0 38.0 40.0 9 39.34425 40.0 40.0 40.0 39.0 40.0 10-14 39.29325 40.0 40.0 40.0 39.0 40.0 15-19 39.1136 40.0 40.0 40.0 38.2 40.0 20-24 39.24015 40.0 40.0 40.0 38.8 40.0 25-29 39.225350000000006 40.0 40.0 40.0 39.0 40.0 30-34 39.14085 40.0 40.0 40.0 38.6 40.0 35-39 39.1953 40.0 40.0 40.0 39.0 40.0 40-44 39.2436 40.0 40.0 40.0 38.8 40.0 45-49 39.15045 40.0 40.0 40.0 38.6 40.0 50-54 39.128499999999995 40.0 40.0 40.0 38.4 40.0 55-59 39.1291 40.0 40.0 40.0 38.4 40.0 60-64 39.0862 40.0 40.0 40.0 38.0 40.0 65-69 39.10895 40.0 40.0 40.0 38.2 40.0 70-74 39.05145 40.0 40.0 40.0 38.2 40.0 75-79 38.951800000000006 40.0 39.4 40.0 37.2 40.0 80-84 38.961349999999996 40.0 39.2 40.0 37.8 40.0 85-89 38.8158 40.0 39.0 40.0 36.8 40.0 90-94 38.93240000000001 40.0 39.0 40.0 37.2 40.0 95-99 38.743199999999995 40.0 39.0 40.0 36.2 40.0 100-104 38.116249999999994 39.4 38.4 39.8 35.4 39.8 105-109 38.7943 40.0 39.0 40.0 36.8 40.0 110-114 38.78435 40.0 39.0 40.0 36.4 40.0 115-119 38.71945 40.0 39.0 40.0 36.4 40.0 120-124 38.5815 40.0 39.0 40.0 36.0 40.0 125-129 38.344 40.0 39.0 40.0 35.6 40.0 130-134 38.2046 40.0 39.0 40.0 35.4 40.0 135-139 38.1471 40.0 39.0 40.0 35.2 40.0 140-144 37.7796 40.0 39.0 40.0 34.4 40.0 145-149 37.14815 40.0 38.8 40.0 33.0 40.0 150-151 34.09475 38.0 35.0 39.5 16.5 40.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 19 1.0 20 2.0 21 0.0 22 2.0 23 2.0 24 5.0 25 7.0 26 7.0 27 21.0 28 14.0 29 25.0 30 30.0 31 39.0 32 42.0 33 46.0 34 79.0 35 87.0 36 127.0 37 189.0 38 362.0 39 2913.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 51.29040340766725 6.890503633174642 2.9315960912052117 38.88749686795289 2 18.998748435544428 7.183979974968711 34.34292866082603 39.474342928660825 3 17.1 10.424999999999999 26.775 45.7 4 23.9 17.45 20.225 38.425 5 26.95302687766893 23.109771414217533 24.039186134137154 25.898015573976384 6 24.2 24.45 25.124999999999996 26.224999999999998 7 17.2 22.8 42.175000000000004 17.825 8 19.5 17.974999999999998 35.3 27.224999999999998 9 19.5 18.3 34.65 27.55 10-14 21.725 22.85 27.794999999999998 27.63 15-19 23.44 20.745 26.39 29.425 20-24 23.724999999999998 22.81 25.685000000000002 27.779999999999998 25-29 23.79 20.82 26.834999999999997 28.555000000000003 30-34 23.885 20.419999999999998 25.505 30.19 35-39 22.68 20.835 27.095000000000002 29.39 40-44 23.055 21.47 24.445 31.03 45-49 24.884999999999998 19.6 24.884999999999998 30.630000000000003 50-54 23.59 20.29 26.625 29.494999999999997 55-59 22.185 21.9 27.065 28.849999999999998 60-64 25.380000000000003 20.044999999999998 26.515 28.060000000000002 65-69 22.435 23.06 25.019999999999996 29.485 70-74 23.419999999999998 22.735 24.740000000000002 29.104999999999997 75-79 24.525 23.48 24.195 27.800000000000004 80-84 22.785 25.03 24.205 27.98 85-89 23.86 23.26 23.5 29.38 90-94 25.72 22.95 22.830000000000002 28.499999999999996 95-99 24.43 22.495 24.725 28.349999999999998 100-104 24.84 22.439999999999998 24.315 28.405 105-109 22.96 22.564999999999998 24.985 29.49 110-114 23.16 23.36 25.28 28.199999999999996 115-119 23.080000000000002 23.535 25.115 28.27 120-124 22.994999999999997 25.895000000000003 22.155 28.955 125-129 22.755 23.189999999999998 23.535 30.520000000000003 130-134 23.82 21.81 23.385 30.985000000000003 135-139 21.675 23.275000000000002 24.21 30.84 140-144 23.235 23.925 23.91 28.93 145-149 21.584999999999997 24.735 24.81 28.87 150-151 20.748810418231905 25.05634861006762 24.693213122965187 29.501627848735286 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.5 23 0.5 24 0.5 25 0.5 26 1.0 27 2.5 28 2.5 29 1.5 30 2.5 31 2.5 32 2.0 33 2.0 34 2.5 35 5.0 36 22.0 37 36.5 38 41.5 39 60.0 40 56.0 41 46.0 42 47.5 43 56.5 44 61.5 45 78.0 46 106.5 47 100.0 48 93.5 49 101.5 50 127.5 51 176.0 52 192.0 53 205.0 54 241.5 55 397.0 56 456.0 57 297.5 58 240.5 59 237.5 60 155.0 61 75.0 62 46.5 63 45.5 64 35.0 65 19.5 66 16.0 67 10.0 68 10.5 69 9.5 70 9.0 71 13.0 72 24.0 73 18.0 74 3.5 75 2.0 76 2.0 77 0.5 78 1.0 79 1.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.22499999999999998 2 0.125 3 0.0 4 0.0 5 0.475 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.17500000000000002 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.675000000000004 #Duplication Level Percentage of deduplicated Percentage of total 1 72.85921625544267 37.65 2 12.23996129656507 12.65 3 4.837929366231252 7.5 4 2.951136913401064 6.1 5 1.6932752781809386 4.375 6 1.257861635220126 3.9 7 1.0643444605708756 3.85 8 0.628930817610063 2.6 9 0.628930817610063 2.9250000000000003 >10 1.741654571843251 15.225 >50 0.09675858732462506 3.225 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GGCATGATGACTTGGCCTCATCCTCTCCTTCCTCCGGCTTAACACCGGCG 70 1.7500000000000002 No Hit CCCCACTGCTGCCTCCCGTAGGAGTCTGGGCCGTGTCTCAGTCCCAGTGT 59 1.4749999999999999 No Hit GTCCAGTCAACTGCTGCGCCTCAACGCATTTCGGGGAGAACCAGCTAGCT 44 1.0999999999999999 No Hit GTCCCTATTAATCATTACTCCGATCCCGAAGGCCAACACAATAGGACCGG 35 0.8750000000000001 No Hit CCCGTCAATTCCTTTGAGTTTCATTCTTGCGAACGTACTCCCCAGGCGGG 32 0.8 No Hit GGGGCATGATGACTTGGCCTCATCCTCTCCTTCCTCCGGCTTAACACCGG 27 0.675 No Hit GTTCTATTTCACTACCCACTGGGGGTTCTTTTCACCTTTCCCTCACGGTA 25 0.625 No Hit CCGGGAACGGATTCACCGCCGTATGGCTGACCGGCGATTACTAGCGATTC 25 0.625 No Hit GTCGGCATCGTTTATGGTTGAGACTAGGACGGTATCTGATCGTCTTCGAG 24 0.6 No Hit CCTCGCGGTACTTGTTCGCTATCGGTCTCTCGCCTGTATTTAGCCTTGGA 24 0.6 No Hit CCCACTGCTGCCTCCCGTAGGAGTCTGGGCCGTGTCTCAGTCCCAGTGTG 24 0.6 No Hit GCTTTCTTTTCCTCTGGCTACTAAGATGTTTCAGTTCGCCAGGTTGTCTC 20 0.5 No Hit GTTCGAGCTTTTCCTGGGAGTATGGCATCGGTTACATACTTCAGTGCCGT 19 0.475 No Hit CCCTAGAGTAACTTTTATCCGTTGAGCGACGGCCCTTCCACTCGGCACCG 19 0.475 No Hit GACCTATTTGGGAATCTCCGGATCTATGCTTATTTTCAACTCCCCGAAGC 18 0.44999999999999996 No Hit GTTCGTTAACGGAATTAACCAGACAAATCGCTCCACCAACTAAGAACGGC 15 0.375 No Hit CTCCACGCGGCATTGCTCCGTCAGGCTTTCGCCCATTGCGGAAAATTCCC 15 0.375 No Hit GTGCTCTGCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTCCTA 14 0.35000000000000003 No Hit GCCCAATCATTCCGGATAACGCTTGCATCCTCTGTCTTACCGCGGCTGCT 14 0.35000000000000003 No Hit GTCGGTTTCGGGTACAGGTACCCTTTTGTTGAAGGTCGTTCGAGCTTTTC 13 0.325 No Hit CCATCGTTTACGGCTAGGACTACTGGGGTCTCTAATCCCATTTGCTCCCC 13 0.325 No Hit GCCACCTACAGACGCTTTACGCCCAATCATTCCGGATAACGCTTGCATCC 13 0.325 No Hit CCTAGCTTTCGTCTCTCAGTGTCAGTGTCGGCCCAGCAGAGTGCTTTCGC 13 0.325 No Hit GCTCCTCAGCCTACGGGGTATTAGCAACCGTTTCCAGTTGTTGTTCCCCT 13 0.325 No Hit CCTCACGGTACTACTTCGCTATCGGTCACCCAGGAGTATTTAGCCTTGCA 12 0.3 No Hit GTCGGTTCGGACCTCTGCTTAGTTTCATCCAAGCTTCATCCTGGTCATGG 12 0.3 No Hit GGTCGTTCGAGCTTTTCCTGGGAGTATGGCATCGGTTACATACTTCAGTG 12 0.3 No Hit CCGTCAATTCCTTTGAGTTTCATTCTTGCGAACGTACTCCCCAGGCGGGA 11 0.27499999999999997 No Hit GTCATTGTTTCTTCTCCGAGAAAAGAAGTTGACGACCCGTAGGCCTTCCA 11 0.27499999999999997 No Hit GTCCTTAAACCTATAACCATCTTTCGGCTAACCTAGCCTCCTCCGTCCCT 11 0.27499999999999997 No Hit CTCTGCCCCTACCGTACTCCAGCTTGGTAGTTTCCACCGCCTGTCCAGGG 11 0.27499999999999997 No Hit CGTCAATTCCTTTGAGTTTCATTCTTGCGAACGTACTCCCCAGGCGGGAT 10 0.25 No Hit CCTGTATTTAGCCTTGGACGGAGTCTACCGCCCGATTTGGGCTGCATTCC 10 0.25 No Hit GTACAAAGGGCAGGGACGTAGTCAACGCGAGCTGATGACTCGCGCTTACT 10 0.25 No Hit CCCGGAACCCAAAGACTTTGATTTCTCATAAGGTGCCGGCGGAGTCCTAT 10 0.25 No Hit GCTCCCCTAGCTTTCGTCTCTCAGTGTCAGTGTCGGCCCAGCAGAGTGCT 10 0.25 No Hit GTTCTTGATTAATGAAAACATCCTTGGCAAATGCTTTCGCAGTTGTTCGT 10 0.25 No Hit CCCATCACGATGAAATTTCCCAAGATTACCCGGGCCTGTCGGCCAAGGCT 10 0.25 No Hit CTCCAGACTACAATTCGGACGGCACGGCCGCCCGATTCTCAAGCTGGGCT 9 0.22499999999999998 No Hit CCTAGATGTCCAGTCAACTGCTGCGCCTCAACGCATTTCGGGGAGAACCA 9 0.22499999999999998 No Hit CCACTGCTGCCTCCCGTAGGAGTCTGGGCCGTGTCTCAGTCCCAGTGTGG 9 0.22499999999999998 No Hit CTTGTGTCCTTAAACCTATAACCATCTTTCGGCTAACCTAGCCTCCTCCG 9 0.22499999999999998 No Hit CCTCAGCCTACGGGGTATTAGCAACCGTTTCCAGTTGTTGTTCCCCTCCC 9 0.22499999999999998 No Hit GGGCATGATGACTTGGCCTCATCCTCTCCTTCCTCCGGCTTAACACCGGC 9 0.22499999999999998 No Hit GTGGCAACTAAACACGAGGGTTGCGCTCGTTGCGAGACTTAACCCAACAC 9 0.22499999999999998 No Hit GCCATTCGCAGTTTCACAGTTCAAATTAGTTCATACTTGCACATGCATGG 9 0.22499999999999998 No Hit GCCCCCGTCAATTCCTTTGAGTTTCATTCTTGCGAACGTACTCCCCAGGC 9 0.22499999999999998 No Hit GACCTGTTGTCCATCGACTACGCCTTTCGGCCTGATCTTAGGCCCTGACT 9 0.22499999999999998 No Hit GTCGAGTTATCATGAATCATCGGATCAGCGAGCAAAGCCCGCGTCAGCCT 9 0.22499999999999998 No Hit GCCCCATGCTACTCGGGTCAGAGCGTAAGCTAGTGATGCTTTCGGCTACT 9 0.22499999999999998 No Hit GTTCCGTTCCCTTAACCAAGCCACTGCCTATGAGTCGCCGGCTCATTCTT 9 0.22499999999999998 No Hit GCCCCGTTCATCTTCAGCGCAAGGGCGCTCGATCAGTGAGCTATTACGCA 8 0.2 No Hit GGCGGTGTGTACAAAGGGCAGGGACGTAGTCAACGCGAGCTGATGACTCG 8 0.2 No Hit GCCGGCTCATTCTTCAACAGGCACGCGGTCAGAGATCACTTTCCCCTCCC 8 0.2 No Hit CCCACCTGTGTCGGTTTCGGGTACAGGTACCCTTTTGTTGAAGGTCGTTC 8 0.2 No Hit GTCAATTCCTTTGAGTTTCATTCTTGCGAACGTACTCCCCAGGCGGGATA 8 0.2 No Hit CCTAGAGTAACTTTTATCCGTTGAGCGACGGCCCTTCCACTCGGCACCGT 8 0.2 No Hit GCCCCATAGAAACTGTCTACCTGAGACTGTCCCTTGGCCCGCGGGTCTGA 8 0.2 No Hit GTCCATCGACTACGCCTTTCGGCCTGATCTTAGGCCCTGACTCACCCTCC 8 0.2 No Hit GTCGTTCGAGCTTTTCCTGGGAGTATGGCATCGGTTACATACTTCAGTGC 8 0.2 No Hit GTTCGCTATCGGTCTCTCGCCTGTATTTAGCCTTGGACGGAGTCTACCGC 8 0.2 No Hit ATTTGGGAATCTCCGGATCTATGCTTATTTTCAACTCCCCGAAGCATTTC 8 0.2 No Hit CTTCGCTATCGGTCACCCAGGAGTATTTAGCCTTGCAAGGTGGTCCTTGC 8 0.2 No Hit CAGGGTTCCAAACTCATAGTGGCAACTAAACACGAGGGTTGCGCTCGTTG 8 0.2 No Hit GCATCGTTTATGGTTGAGACTAGGACGGTATCTGATCGTCTTCGAGCCCC 7 0.17500000000000002 No Hit GTTCCATTGGCCAGAGGCTGTTCACCTTGGAGACCTGATGCGGTTATGAG 7 0.17500000000000002 No Hit GTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTC 7 0.17500000000000002 No Hit ATCGACTACGCCTTTCGGCCTGATCTTAGGCCCTGACTCACCCTCCGTGG 7 0.17500000000000002 No Hit GGCATCACAGACCTGTTATTGCCTCAAACTTCCGTCGCCTAAACGGCGAT 7 0.17500000000000002 No Hit GTACCGCATTAATGGGCGAACAGCCCAACCCTTGGAACCACCTACAGCTC 7 0.17500000000000002 No Hit CTCCCATTTCGCTCGCCGCTACTACGGGAATCGCTTTTGCTTTCTTTTCC 7 0.17500000000000002 No Hit GTTCCCTTAACCAAGCCACTGCCTATGAGTCGCCGGCTCATTCTTCAACA 7 0.17500000000000002 No Hit GCACGTACCATCAAACAAACTATAACTGATTTAATGAGCCATTCGCAGTT 7 0.17500000000000002 No Hit CCCGTTTTCACGGTTTAGGCTGCTCCCATTTCGCTCGCCGCTACTACGGG 7 0.17500000000000002 No Hit GTGTACAAAGGGCAGGGACGTAGTCAACGCGAGCTGATGACTCGCGCTTA 7 0.17500000000000002 No Hit GTCCCAGTGTGGCTGATCATCCTCTCGGACCAGCTACTGATCATCGCCTT 7 0.17500000000000002 No Hit CATCGTTTACGGCTAGGACTACTGGGGTCTCTAATCCCATTTGCTCCCCT 7 0.17500000000000002 No Hit CTCTCAGTGTCAGTGTCGGCCCAGCAGAGTGCTTTCGCCGTTGGTGTTCT 7 0.17500000000000002 No Hit ATCACGATGAAATTTCCCAAGATTACCCGGGCCTGTCGGCCAAGGCTATA 7 0.17500000000000002 No Hit CCGGAACCCAAAGACTTTGATTTCTCATAAGGTGCCGGCGGAGTCCTATA 7 0.17500000000000002 No Hit GGCTTGCTTTGAGCACTCTAATTTCTTCAAAGTAACGATGCCGGAGGCAC 7 0.17500000000000002 No Hit CCTGTGTCGGTTTCGGGTACAGGTACCCTTTTGTTGAAGGTCGTTCGAGC 7 0.17500000000000002 No Hit GCTCATTCTTCAACAGGCACGCGGTCAGAGATCACTTTCCCCTCCCACTG 7 0.17500000000000002 No Hit CTTTCTTTTCCTCTGGCTACTAAGATGTTTCAGTTCGCCAGGTTGTCTCT 7 0.17500000000000002 No Hit GTCTGTTCAGGGTTCCAAACTCATAGTGGCAACTAAACACGAGGGTTGCG 7 0.17500000000000002 No Hit GGGGAAGGGAGCTTCGAGGCGGCCGGACGCGGCTCGTCGGCCGGAACGGC 7 0.17500000000000002 No Hit GCACGGTTTCACGTTCTATTTCACTACCCACTGGGGGTTCTTTTCACCTT 6 0.15 No Hit GTGTGTACAAAGGGCAGGGACGTAGTCAACGCGAGCTGATGACTCGCGCT 6 0.15 No Hit GGCCAAAATAACCATGAGCAGCCACAATATTATAAGTCTCTTCCTCTTGA 6 0.15 No Hit GGCATAAGGGGCATGATGACTTGGCCTCATCCTCTCCTTCCTCCGGCTTA 6 0.15 No Hit GCTCCGTCAGGCTTTCGCCCATTGCGGAAAATTCCCCACTGCTGCCTCCC 6 0.15 No Hit CCCTTGTCCGTACCAGTTCTGAGTCGACTGTTCAGCGCTCGGGGAAAGCC 6 0.15 No Hit CCAGTCAACTGCTGCGCCTCAACGCATTTCGGGGAGAACCAGCTAGCTCT 6 0.15 No Hit GGGCATCACAGACCTGTTATTGCCTCAAACTTCCGTCGCCTAAACGGCGA 6 0.15 No Hit GTCTCTCAGTGTCAGTGTCGGCCCAGCAGAGTGCTTTCGCCGTTGGTGTT 6 0.15 No Hit GCATGATGACTTGGCCTCATCCTCTCCTTCCTCCGGCTTAACACCGGCGG 6 0.15 No Hit GCTGCTGGCACAGAGTTAGCCGATGCTTATTCCTCAGATACCGTCATTGT 6 0.15 No Hit CCTAATTCTCCGTCACCCGTCACCACCATGGTAGGCCCCTATCCTACCAT 6 0.15 No Hit CCCTACCGTACTCCAGCTTGGTAGTTTCCACCGCCTGTCCAGGGTTGAGC 6 0.15 No Hit GTCCTCTCAATGCTCTAACGCCCACACCGGATATGGACCGAACTGTCTCA 6 0.15 No Hit ATCGTTTACGGCTAGGACTACTGGGGTCTCTAATCCCATTTGCTCCCCTA 6 0.15 No Hit GTTCAGGGTTCCAAACTCATAGTGGCAACTAAACACGAGGGTTGCGCTCG 6 0.15 No Hit GCCCCTACCGTACTCCAGCTTGGTAGTTTCCACCGCCTGTCCAGGGTTGA 6 0.15 No Hit GCGGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTACG 6 0.15 No Hit CTCAGATACCGTCATTGTTTCTTCTCCGAGAAAAGAAGTTGACGACCCGT 6 0.15 No Hit CCCGTGTCAGGATTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTG 6 0.15 No Hit CGTCATTGTTTCTTCTCCGAGAAAAGAAGTTGACGACCCGTAGGCCTTCC 6 0.15 No Hit GCACGTGTGTCGCCCAGGGCATAAGGGGCATGATGACTTGGCCTCATCCT 6 0.15 No Hit GCCCGATTTGGGCTGCATTCCCAAACAACCCGACTCGTTGACGGCGCCTC 6 0.15 No Hit GCCTCCCGTAGGAGTCTGGGCCGTGTCTCAGTCCCAGTGTGGCTGATCAT 6 0.15 No Hit GTCGCGTATTTAAGTCGTCTGCAAAGGATTCAGCCCGCCGCCCGTGGGGA 6 0.15 No Hit GTGGGCTTACTACTTATATGCTTTCAGCAGTTATCCTCTCCGCACTTGGC 6 0.15 No Hit CTTTATCACTGAGCGGTCATTTAGGGGCCTTAGCTGGTGATCCGGGCTGT 5 0.125 No Hit CGTTGAGCGACGGCCCTTCCACTCGGCACCGTCGGATCACTAAGGCCGAC 5 0.125 No Hit GGGAAGGGAGCTTCGAGGCGGCCGGACGCGGCTCGTCGGCCGGAACGGCT 5 0.125 No Hit AGCGCGCGTGCGGCCCAGAACATCTAAGGGCATCACAGACCTGTTATTGC 5 0.125 No Hit CTTCCGTCAATTCCTTTAAGTTTCAGCCTTGCGACCATACTCCCCCCGGA 5 0.125 No Hit CTCACGTACCGCATTAATGGGCGAACAGCCCAACCCTTGGAACCACCTAC 5 0.125 No Hit GCGGTGTGTACAAAGGGCAGGGACGTAGTCAACGCGAGCTGATGACTCGC 5 0.125 No Hit GTCTGACACAAGGTTAGAATCCGAGCTCTTCCAGAGTGGTATCTCACTGA 5 0.125 No Hit GGCGATAATAAATACAGAAGTTGCGGTCAATAAGGTAGGGATCATCAAAA 5 0.125 No Hit GGGGTGGGCTTACTACTTATATGCTTTCAGCAGTTATCCTCTCCGCACTT 5 0.125 No Hit CTCACGGTACTACTTCGCTATCGGTCACCCAGGAGTATTTAGCCTTGCAA 5 0.125 No Hit GGTCGGGGCAGGCGGCGGGCGCAGGCGCCGCTTGCTAGCTTGGATTCTGA 5 0.125 No Hit GGGCGGTGTGTACAAAGGGCAGGGACGTAGTCAACGCGAGCTGATGACTC 5 0.125 No Hit CCTATTTGGGAATCTCCGGATCTATGCTTATTTTCAACTCCCCGAAGCAT 5 0.125 No Hit CTGGGAGTATGGCATCGGTTACATACTTCAGTGCCGTAGCGCCTGGTATG 5 0.125 No Hit CCTCAACGCATTTCGGGGAGAACCAGCTAGCTCTGGGTTCGAGTGGCATT 5 0.125 No Hit GTACCGCTCGCGCAGCCCGCACCGAAACAGTGCTTTACCCCTAGATGTCC 5 0.125 No Hit CTTAAACCTATAACCATCTTTCGGCTAACCTAGCCTCCTCCGTCCCTCCG 5 0.125 No Hit GCCTGTTATCCCTAGAGTAACTTTTATCCGTTGAGCGACGGCCCTTCCAC 5 0.125 No Hit CTCCTTTTGCTCCTCAGCCTACGGGGTATTAGCAACCGTTTCCAGTTGTT 5 0.125 No Hit CCTCTCCGCACTTGGCTACCCAGCGTTTACCGTAGGCACGATAACTGGTA 5 0.125 No Hit GTGTCCTTAAACCTATAACCATCTTTCGGCTAACCTAGCCTCCTCCGTCC 5 0.125 No Hit CATCCCACAGCTTCGGCAGATCGCTTAGCCCCGTTCATCTTCAGCGCAAG 5 0.125 No Hit GCATCCTCTGTCTTACCGCGGCTGCTGGCACAGAGTTAGCCGATGCTTAT 5 0.125 No Hit CTAGCTTTCGTCTCTCAGTGTCAGTGTCGGCCCAGCAGAGTGCTTTCGCC 5 0.125 No Hit GCCCTATGAAGACTCGCTTTCGCTACGGCTCCGGTGGGTTCCGTTCCCTT 5 0.125 No Hit GCTCACGTACCGCATTAATGGGCGAACAGCCCAACCCTTGGAACCACCTA 5 0.125 No Hit AGCACGTGTGTCGCCCAGGGCATAAGGGGCATGATGACTTGGCCTCATCC 5 0.125 No Hit CGCCCAATCATTCCGGATAACGCTTGCATCCTCTGTCTTACCGCGGCTGC 5 0.125 No Hit CCCAACTTTCGTTCTTGATTAATGAAAACATCCTTGGCAAATGCTTTCGC 5 0.125 No Hit GGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTACGTT 5 0.125 No Hit GGCTCATTCTTCAACAGGCACGCGGTCAGAGATCACTTTCCCCTCCCACT 5 0.125 No Hit GCTTGCTTTGAGCACTCTAATTTCTTCAAAGTAACGATGCCGGAGGCACG 5 0.125 No Hit GCTCTGCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTCCTAAA 5 0.125 No Hit CCGGGCTGTTTCCCTCTCGACGATGAAGCTTATCCCCCATCGTCTCACTG 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0125 0.0 0.0 0.0 0.0 26-27 0.025 0.0 0.0 0.0 0.0 28-29 0.025 0.0 0.0 0.0 0.0 30-31 0.025 0.0 0.0 0.0 0.0 32-33 0.025 0.0 0.0 0.0 0.0 34-35 0.025 0.0 0.0 0.0 0.0 36-37 0.025 0.0 0.0 0.0 0.0 38-39 0.025 0.0 0.0 0.0 0.0 40-41 0.025 0.0 0.0 0.0 0.0 42-43 0.037500000000000006 0.0 0.0 0.0 0.0 44-45 0.075 0.0 0.0 0.0 0.0 46-47 0.1 0.0 0.0 0.0 0.0 48-49 0.125 0.0 0.0 0.0 0.0 50-51 0.15 0.0 0.0 0.0 0.0 52-53 0.175 0.0 0.0 0.0 0.0 54-55 0.2375 0.0 0.0 0.0 0.0 56-57 0.275 0.0 0.0 0.0 0.0 58-59 0.3125 0.0 0.0 0.0 0.0 60-61 0.375 0.0 0.0 0.0 0.0 62-63 0.4625 0.0 0.0 0.0 0.0 64-65 0.625 0.0 0.0 0.0 0.0 66-67 0.7124999999999999 0.0 0.0 0.0 0.0 68-69 0.7875000000000001 0.0 0.0 0.0 0.0 70-71 1.05 0.0 0.0 0.0 0.0 72-73 1.3125 0.0 0.0 0.0 0.0 74-75 1.6875 0.0 0.0 0.0 0.0 76-77 2.0375 0.0 0.0 0.0 0.0 78-79 2.4625000000000004 0.0 0.0 0.0 0.0 80-81 2.8375000000000004 0.0 0.0 0.0 0.0 82-83 3.2625 0.0 0.0 0.0 0.0 84-85 3.9625000000000004 0.0 0.0 0.0 0.0 86-87 4.699999999999999 0.0 0.0 0.0 0.0 88-89 5.7 0.0 0.0 0.0 0.0 90-91 6.4625 0.0 0.0 0.0 0.0 92-93 7.175 0.0 0.0 0.0 0.0 94-95 7.975 0.0 0.0 0.0 0.0 96-97 9.0 0.0 0.0 0.0 0.0 98-99 9.9375 0.0 0.0 0.0 0.0 100-101 10.912500000000001 0.0 0.0 0.0 0.0 102-103 11.9875 0.0 0.0 0.0 0.0 104-105 12.9375 0.0 0.0 0.0 0.0 106-107 13.8875 0.0 0.0 0.0 0.0 108-109 15.4 0.0 0.0 0.0 0.0 110-111 16.675 0.0 0.0 0.0 0.0 112-113 18.075000000000003 0.0 0.0 0.0 0.0 114-115 19.3125 0.0 0.0 0.0 0.0 116-117 20.675 0.0 0.0 0.0 0.0 118-119 21.9625 0.0 0.0 0.0 0.0 120-121 23.0 0.0 0.0 0.0 0.0 122-123 24.137500000000003 0.0 0.0 0.0 0.0 124-125 25.475 0.0 0.0 0.0 0.0 126-127 26.612499999999997 0.0 0.0 0.0 0.0 128-129 27.7125 0.0 0.0 0.0 0.0 130-131 28.637500000000003 0.0 0.0 0.0 0.0 132-133 29.575 0.0 0.0 0.0 0.0 134-135 30.549999999999997 0.0 0.0 0.0 0.0 136-137 31.4625 0.0 0.0 0.0 0.0 138-139 32.6125 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCCTCCG 20 0.00593511 29.0 30-34 TCCTTCC 20 0.00593511 29.0 25-29 GCCTCAT 20 0.00593511 29.0 15-19 GGTCTGT 20 0.00593511 29.0 50-54 CACCGGC 20 0.00593511 29.0 40-44 CTCTCCT 20 0.00593511 29.0 20-24 GTCTGTT 20 0.00593511 29.0 50-54 ACACCGG 20 0.00593511 29.0 40-44 CGGCGGT 20 0.00593511 29.0 45-49 CCTCCGG 20 0.00593511 29.0 30-34 TCCGGCT 20 0.00593511 29.0 30-34 CCTCATC 20 0.00593511 29.0 15-19 TAACACC 20 0.00593511 29.0 40-44 CGGCTTA 20 0.00593511 29.0 35-39 CTTAACA 20 0.00593511 29.0 35-39 GGCGGTC 20 0.00593511 29.0 45-49 CTCCGGC 20 0.00593511 29.0 30-34 AACACCG 20 0.00593511 29.0 40-44 GCTTAAC 20 0.00593511 29.0 35-39 AAAAAAA 115 2.8485374E-9 18.913044 145 >>END_MODULE SRR6941552 read2 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR6941552_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 52 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 34.1195 35.0 35.0 35.0 33.0 35.0 2 34.31025 35.0 35.0 35.0 33.0 35.0 3 34.307 35.0 35.0 35.0 33.0 35.0 4 34.24425 35.0 35.0 35.0 33.0 35.0 5 34.3335 35.0 35.0 35.0 33.0 35.0 6 39.0895 40.0 40.0 40.0 38.0 40.0 7 39.05575 40.0 40.0 40.0 38.0 40.0 8 39.149 40.0 40.0 40.0 39.0 40.0 9 39.1445 40.0 40.0 40.0 39.0 40.0 10-14 39.17470000000001 40.0 40.0 40.0 38.6 40.0 15-19 39.1712 40.0 40.0 40.0 39.0 40.0 20-24 39.1736 40.0 40.0 40.0 38.8 40.0 25-29 39.1662 40.0 40.0 40.0 39.0 40.0 30-34 39.080200000000005 40.0 39.8 40.0 38.4 40.0 35-39 39.155150000000006 40.0 40.0 40.0 38.8 40.0 40-44 38.97515 40.0 39.8 40.0 38.0 40.0 45-49 38.814800000000005 40.0 39.2 40.0 37.4 40.0 50-54 38.808299999999996 40.0 39.0 40.0 37.2 40.0 55-59 38.8604 40.0 39.0 40.0 37.6 40.0 60-64 38.85625 40.0 39.0 40.0 37.6 40.0 65-69 38.7138 40.0 39.0 40.0 36.6 40.0 70-74 38.72099999999999 40.0 39.0 40.0 36.6 40.0 75-79 38.57265 40.0 39.0 40.0 36.2 40.0 80-84 38.600649999999995 40.0 39.0 40.0 36.0 40.0 85-89 38.50445 40.0 39.0 40.0 36.0 40.0 90-94 38.468849999999996 40.0 39.0 40.0 36.0 40.0 95-99 38.26215 40.0 39.0 40.0 35.6 40.0 100-104 37.3368 39.0 38.0 39.6 32.8 39.8 105-109 37.89895 40.0 39.0 40.0 34.6 40.0 110-114 34.50425 36.4 34.2 38.2 28.6 38.6 115-119 18.426700000000004 16.6 15.6 23.2 13.4 30.0 120-124 2.0 2.0 2.0 2.0 2.0 2.0 125-129 2.0 2.0 2.0 2.0 2.0 2.0 130-134 2.0 2.0 2.0 2.0 2.0 2.0 135-139 2.0 2.0 2.0 2.0 2.0 2.0 140-144 2.0 2.0 2.0 2.0 2.0 2.0 145-149 2.0 2.0 2.0 2.0 2.0 2.0 150-151 2.0 2.0 2.0 2.0 2.0 2.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 1.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 3.0 10 1.0 11 1.0 12 2.0 13 1.0 14 3.0 15 3.0 16 2.0 17 6.0 18 5.0 19 9.0 20 10.0 21 21.0 22 22.0 23 30.0 24 48.0 25 55.0 26 71.0 27 121.0 28 176.0 29 315.0 30 2096.0 31 995.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 53.00751879699248 17.468671679197996 4.135338345864661 25.38847117794486 2 32.90758827948911 22.188830453293264 24.417731029301276 20.485850237916353 3 26.289434151226843 21.732598898347522 28.743114672008012 23.234852278417627 4 28.95066366140746 29.451540195341845 20.686200851490106 20.91159529176058 5 32.78236914600551 30.929125970448286 16.779363886801903 19.509140996744303 6 26.25 36.1 16.275000000000002 21.375 7 24.95 22.825 29.825000000000003 22.400000000000002 8 27.400000000000002 21.5 22.475 28.625 9 29.175 19.875 25.35 25.6 10-14 29.365000000000002 25.169999999999998 21.23 24.235 15-19 29.82 25.36 21.325 23.494999999999997 20-24 29.555 24.73 22.275 23.44 25-29 30.135 25.695 21.41 22.759999999999998 30-34 29.220000000000002 25.53 21.435000000000002 23.815 35-39 29.67445116767515 26.75901385207781 20.37305595839376 23.19347902185328 40-44 29.612403100775193 26.151537884471114 21.69542385596399 22.540635158789698 45-49 29.258517034068138 26.57815631262525 20.671342685370742 23.491983967935873 50-54 30.12615138165799 24.819783740488585 21.57589106928314 23.478173808570286 55-59 28.92549569397156 26.336871620268376 21.570198277588624 23.167434408171438 60-64 30.183674490766226 24.88363945748461 22.281167108753316 22.651518942995846 65-69 30.130130130130127 25.275275275275277 21.436436436436438 23.158158158158155 70-74 30.792713442097885 24.977479731758585 21.589430487438698 22.640376338704833 75-79 30.26105221044209 25.250050010002 21.149229845969195 23.339667933586718 80-84 29.865906134294008 25.60792554788352 21.935354748323828 22.59081356949865 85-89 30.458045804580458 25.332533253325334 21.227122712271225 22.98229822982298 90-94 30.625000000000004 25.575 20.630000000000003 23.169999999999998 95-99 31.290000000000003 25.435000000000002 20.93 22.345000000000002 100-104 30.438043804380438 27.2977297729773 20.482048204820483 21.782178217821784 105-109 31.585 25.505 21.315 21.595 110-114 30.62932157530815 26.12987273273875 21.56027658081972 21.68052911113338 115-119 32.2045611610228 25.651818810077277 20.86448451341333 21.279135515486587 120-124 28.15139391630152 41.33485743316187 14.072521750174635 16.441226900361976 125-129 NaN NaN NaN NaN 130-134 32.084956641950484 27.196179464622343 19.303757697624732 21.415106195802437 135-139 31.085220710088883 25.837392658062576 21.41816903530357 21.659217596544973 140-144 32.99854715431748 25.753268902785674 20.876760785800013 20.371423157096835 145-149 32.49061326658323 26.307884856070086 19.44931163954944 21.752190237797247 150-151 32.30615056996117 26.706751847676312 19.879744456971064 21.107353125391455 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.5 20 0.5 21 0.0 22 0.0 23 0.0 24 0.0 25 0.5 26 0.5 27 1.0 28 1.5 29 1.5 30 2.0 31 2.5 32 3.0 33 4.0 34 8.0 35 19.0 36 24.5 37 26.0 38 35.0 39 51.0 40 57.0 41 49.0 42 47.5 43 65.5 44 79.0 45 85.0 46 85.0 47 89.0 48 98.0 49 109.0 50 125.0 51 166.5 52 177.5 53 211.5 54 341.0 55 408.5 56 364.0 57 268.5 58 198.5 59 174.0 60 139.0 61 92.5 62 80.0 63 57.5 64 26.5 65 18.5 66 14.5 67 14.5 68 28.5 69 36.5 70 28.0 71 20.5 72 11.5 73 6.0 74 8.5 75 9.5 76 9.5 77 8.5 78 5.5 79 3.0 80 1.0 81 0.5 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 0.25 2 0.17500000000000002 3 0.15 4 0.17500000000000002 5 0.17500000000000002 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.015 40-44 0.025 45-49 0.2 50-54 0.12 55-59 0.13999999999999999 60-64 0.095 65-69 0.1 70-74 0.09 75-79 0.02 80-84 0.06999999999999999 85-89 0.01 90-94 0.0 95-99 0.0 100-104 0.01 105-109 0.0 110-114 0.21 115-119 20.415 120-124 21.265 125-129 100.0 130-134 60.214999999999996 135-139 0.43499999999999994 140-144 20.845 145-149 80.025 150-151 0.21250000000000002 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.050000000000004 #Duplication Level Percentage of deduplicated Percentage of total 1 71.6760676873489 44.474999999999994 2 15.350523771152297 19.05 3 6.4464141821112 12.0 4 2.9008863819500403 7.199999999999999 5 1.5310233682514103 4.75 6 0.7252215954875101 2.7 7 0.5640612409347301 2.45 8 0.08058017727639001 0.4 9 0.201450443190975 1.125 >10 0.523771152296535 5.8500000000000005 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTTCGATCCTGGCTCAGGATGAACGCTGGCGGCATGCTTAACACATGCAA 40 1.0 No Hit CCTTACCAGGGCTTGACATGCCGCGAATCCTCTTGAAAGAGAGGGGTGCC 34 0.8500000000000001 No Hit CCTGCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGCAA 30 0.75 No Hit GGCTTGCGGTGGATACCTAGGCACCCAGAGACGAGGAAGGGCGTAGCAAG 28 0.7000000000000001 No Hit GAAACAATGACGGTATCTGAGGAATAAGCATCGGCTAACTCTGTGCCAGC 16 0.4 No Hit GCCGCGAATCCTCTTGAAAGAGAGGGGTGCCCTCGGGAACGCGGACACAG 13 0.325 No Hit GGCAGAGGGAATTTCCGGTGGAGCGGTGAAATGCATTGAGATCGGAAAGA 12 0.3 No Hit GATGAACGCTGGCGGCATGCTTAACACATGCAAGTCGAACGGGAAGTGGT 11 0.27499999999999997 No Hit GTTGCTAATACCCCGTAGGCTGAGGAGCAAAAGGAGAAATCCGCCCAAGG 10 0.25 No Hit GGGAGCTTGACTGCAAGACTCACCCGTCGAGCAGAGACGAAAGTCGGCCT 10 0.25 No Hit GAGCAATACAAGCGTTGTGCTGCTAGGCGAAGCGGTTGAGTGCCGCACCC 10 0.25 No Hit CAGTGACCAGGCCCGGGCGACTGTTTACCAAAAACACAGGTCTCCGCAAA 10 0.25 No Hit GTTAGTTTTACCCTACTGATGACCGTGCCGCGATAGTAATTCAACCTAGT 10 0.25 No Hit GAACCTTACCAGGGCTTGACATGCCGCGAATCCTCTTGAAAGAGAGGGGT 9 0.22499999999999998 No Hit ACCAGGTCCAGACATAGCAAGGATTGACAGACTGAGAGCTCTTTCTTGAT 9 0.22499999999999998 No Hit GGCGGGACTACCCGCTGAGTTTAAGCATATAAATAAGCGGAGGAGAAGAA 9 0.22499999999999998 No Hit GTGAAATTCTTGGATTTATGAAAGACGAACAACTGCGAAAGCATTTGCCA 9 0.22499999999999998 No Hit CCTAGGCACCCAGAGACGAGGAAGGGCGTAGCAAGCGACGAAATGCTTCG 9 0.22499999999999998 No Hit GTTGGACTTTGGGCCGGGTCGGCCGGTCCGCCTCACGGCGAGCACCGACC 8 0.2 No Hit AGCGTCTGTAGGTGGCTTTTCAAGTCCGCCGTCAAATCCCAGGGCTCAAC 8 0.2 No Hit CAGGGCTTGACATGCCGCGAATCCTCTTGAAAGAGAGGGGTGCCCTCGGG 7 0.17500000000000002 No Hit GGCTGTCGTCAGCTCGTGCCGTAAGGTGTTGGGTTAAGTCTCGCAACGAG 7 0.17500000000000002 No Hit GGAATTTCCGGTGGAGCGGTGAAATGCATTGAGATCGGAAAGAACACCAA 7 0.17500000000000002 No Hit AGGTGACCCTGCTTTTTCAGGGTAAGAAGGGGTAGAGAAAATGCCTCGAG 7 0.17500000000000002 No Hit GTTAAGTCTCGCAACGAGCGCAACCCTCGTGTTTAGTTGCCACTATGAGT 7 0.17500000000000002 No Hit GGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGTTAACGAA 7 0.17500000000000002 No Hit GCTTGCGGTGGATACCTAGGCACCCAGAGACGAGGAAGGGCGTAGCAAGC 7 0.17500000000000002 No Hit GCATCGGCTAACTCTGTGCCAGCAGCCGCGGTAAGACAGAGGATGCAAGC 7 0.17500000000000002 No Hit GCTTGTTATATGGGTCGTGAGTGGGAACTTAGTTTCCGTTTGGGTATGCG 7 0.17500000000000002 No Hit CTTACCAGGGCTTGACATGCCGCGAATCCTCTTGAAAGAGAGGGGTGCCC 7 0.17500000000000002 No Hit CTTTGGGCCGGGTCGGCCGGTCCGCCTCACGGCGAGCACCGACCTACTCG 7 0.17500000000000002 No Hit CGCGAATCCTCTTGAAAGAGAGGGGTGCCCTCGGGAACGCGGACACAGGT 7 0.17500000000000002 No Hit GCCCCTTATGCCCTGGGCGACACACGTGCTACAATGGGCGGGACAAAGGG 7 0.17500000000000002 No Hit AGAACCTTACCAGGGCTTGACATGCCGCGAATCCTCTTGAAAGAGAGGGG 7 0.17500000000000002 No Hit CGGACACAGGTGGTGCATGGCTGTCGTCAGCTCGTGCCGTAAGGTGTTGG 6 0.15 No Hit GCGAAAGCCTGACGGAGCAATGCCGCGTGGAGGTGGAAGGCCTACGGGTC 6 0.15 No Hit GTTTAAGGACACAAGGTGACCCTGCTTTTTCAGGGTAAGAAGGGGTAGAG 6 0.15 No Hit CTGAGGAATAAGCATCGGCTAACTCTGTGCCAGCAGCCGCGGTAAGACAG 6 0.15 No Hit GTTCTGGGCCGCACGCGCGCTACACTGATGTATTCAACGAGTATATAGCC 6 0.15 No Hit GGATGAAGCTTGGATGAAACTAAGCAGAGGTCCGAACCGACTGATGTTGA 6 0.15 No Hit CAATAGCTTACCAAGGCGATGATCAGTAGCTGGTCCGAGAGGATGATCAG 6 0.15 No Hit AGGGAATTTCCGGTGGAGCGGTGAAATGCATTGAGATCGGAAAGAACACC 6 0.15 No Hit ACCGGGCCCAAGTCCCCTGGAAAGGGGCGCCTGGGAGGGTGAGAGCCCCG 6 0.15 No Hit GTGAGAGCCCCGTCCGGCCCGGACCCTGTCGCCCCACGAGGCGCCGTCAA 6 0.15 No Hit CTTACCAGGTCCAGACATAGCAAGGATTGACAGACTGAGAGCTCTTTCTT 6 0.15 No Hit GTCGGCTTGAGTAACGAAAACATTGGTGAGAATCCAATGCCCCGAAAACC 6 0.15 No Hit GTTGGGTTAAGTCTCGCAACGAGCGCAACCCTCGTGTTTAGTTGCCACTA 6 0.15 No Hit AGTGACCAGGCCCGGGCGACTGTTTACCAAAAACACAGGTCTCCGCAAAG 6 0.15 No Hit GTTGGGGGCTCGAAGACGATCAGATACCGTCCTAGTCTCAACCATAAACG 6 0.15 No Hit CAGCAAGGACCACCTTGCAAGGCTAAATACTCCTGGGTGACCGATAGCGA 6 0.15 No Hit GCTTAACACATGCAAGTCGAACGGGAAGTGGTGTTTCCAGTGGCGAACGG 6 0.15 No Hit GCTGGTTCTCCCCGAAATGCGTTGAGGCGCAGCAGTTGACTGGACATCTA 6 0.15 No Hit GGCTGATCTTCCCCAAGAGTCCACATCGACGGGAAGGTTTGGCACCTCGA 5 0.125 No Hit CCATAAACGATGCCGACCAGGGATCGGCGGATGTTGCTTATAGGACTCCG 5 0.125 No Hit GCCGAAAGCATCACTAGCTTACGCTCTGACCCGAGTAGCATGGGGCACGT 5 0.125 No Hit GGATGAACGCTGGCGGCATGCTTAACACATGCAAGTCGAACGGGAAGTGG 5 0.125 No Hit CCTGGCTCAGGATGAACGCTGGCGGCATGCTTAACACATGCAAGTCGAAC 5 0.125 No Hit GTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGCAAGTATGAACTA 5 0.125 No Hit GTTGAAGAATGAGCCGGCGACTCATAGGCAGTGGCTTGGTTAAGGGAACG 5 0.125 No Hit GTCAGGCGGGACTACCCGCTGAGTTTAAGCATATAAATAAGCGGAGGAGA 5 0.125 No Hit CTTGCGGTGGATACCTAGGCACCCAGAGACGAGGAAGGGCGTAGCAAGCG 5 0.125 No Hit GGAAAGGGGCGCCTGGGAGGGTGAGAGCCCCGTCCGGCCCGGACCCTGTC 5 0.125 No Hit GGTGTTTCCAGTGGCGAACGGGTGAGTAACGCGTAAGAACCTGCCCTTGG 5 0.125 No Hit GTTGAAAATAAGCATAGATCCGGAGATTCCCAAATAGGTCAACCTTTTAA 5 0.125 No Hit GACGAAAGCTAGGGGAGCAAATGGGATTAGAGACCCCAGTAGTCCTAGCC 5 0.125 No Hit GGTAGCGAAATTCCTTGTCGGGTAAGTTCCGACCCGCACGAAAGGCGTAA 5 0.125 No Hit GTGAGATACCACTCTGGAAGAGCTCGGATTCTAACCTTGTGTCAGACCCG 5 0.125 No Hit GGAACAACAACTGGAAACGGTTGCTAATACCCCGTAGGCTGAGGAGCAAA 5 0.125 No Hit GGAAGGCCTACGGGTCGTCAACTTCTTTTCTCGGAGAAGAAACAATGACG 5 0.125 No Hit GCTTGGATGAAACTAAGCAGAGGTCCGAACCGACTGATGTTGAAGAATCA 5 0.125 No Hit GTGAGACGATGGGGGATAAGCTTCATCGTCGAGAGGGAAACAGCCCGGAT 5 0.125 No Hit GAGATTCCCAAATAGGTCAACCTTTTAAACTGCCTGCTGAATCCATGAGC 5 0.125 No Hit CGAGAATGTCGGCTTGAGTAACGAAAACATTGGTGAGAATCCAATGCCCC 5 0.125 No Hit ATCCGCCCAAGGAGGGGCTCGCGTCTGATTAGCTAGTTGGTGAGGCAATA 5 0.125 No Hit GCAACCGCGAAAGCGGGGGTCGACGAAGCGGAAGCGAGAATGTCGGCTTG 5 0.125 No Hit GTGGGAACTTAGTTTCCGTTTGGGTATGCGCCCTTGGATTGCTGTTGCAT 5 0.125 No Hit GAGCGATTTGTCTGGTTAATTCCGTTAACGAACGAGACCTCAGCCTGCTA 5 0.125 No Hit GTGGAATCCCGTGTGAATCAGCAAGGACCACCTTGCAAGGCTAAATACTC 5 0.125 No Hit AGAGACCCCAGTAGTCCTAGCCGTAAACGATGGATACTAGGTGCTGTGCG 5 0.125 No Hit CAAGCGTTGTGCTGCTAGGCGAAGCGGTTGAGTGCCGCACCCTAGATGGC 5 0.125 No Hit ATTATCTATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAAT 5 0.125 No Hit GTGAAATAGAACGTGAAACCGTGCTGAGCTCCCAAGCAGTGGGAGGGGAA 5 0.125 No Hit CCCAGCTTGAGAATCGGGCGGCCGTGCCGTCCGAATTGTAGTCTGGAGAG 5 0.125 No Hit GTCTGATTAGCTAGTTGGTGAGGCAATAGCTTACCAAGGCGATGATCAGT 5 0.125 No Hit GGACTTTGGGCCGGGTCGGCCGGTCCGCCTCACGGCGAGCACCGACCTAC 5 0.125 No Hit GTTACTTTGAAGAAATTAGAGTGCTCAAAGCAAGCCATCGCTCTGGATAC 5 0.125 No Hit GAGCAAATGGGATTAGAGACCCCAGTAGTCCTAGCCGTAAACGATGGATA 5 0.125 No Hit GAATCCAATGCCCCGAAAACCCAAGGTTTCCTCCGCAAGGTTCGTCCACG 5 0.125 No Hit AGGAGGGGCTCGCGTCTGATTAGCTAGTTGGTGAGGCAATAGCTTACCAA 5 0.125 No Hit GCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGCAAGTA 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0125 0.0 0.0 0.0 0.0 26-27 0.025 0.0 0.0 0.0 0.0 28-29 0.025 0.0 0.0 0.0 0.0 30-31 0.025 0.0 0.0 0.0 0.0 32-33 0.025 0.0 0.0 0.0 0.0 34-35 0.025 0.0 0.0 0.0 0.0 36-37 0.025 0.0 0.0 0.0 0.0 38-39 0.025 0.0 0.0 0.0 0.0 40-41 0.025 0.0 0.0 0.0 0.0 42-43 0.037500000000000006 0.0 0.0 0.0 0.0 44-45 0.075 0.0 0.0 0.0 0.0 46-47 0.075 0.0 0.0 0.0 0.0 48-49 0.1 0.0 0.0 0.0 0.0 50-51 0.125 0.0 0.0 0.0 0.0 52-53 0.15 0.0 0.0 0.0 0.0 54-55 0.21250000000000002 0.0 0.0 0.0 0.0 56-57 0.25 0.0 0.0 0.0 0.0 58-59 0.2875 0.0 0.0 0.0 0.0 60-61 0.35 0.0 0.0 0.0 0.0 62-63 0.4375 0.0 0.0 0.0 0.0 64-65 0.6 0.0 0.0 0.0 0.0 66-67 0.6875 0.0 0.0 0.0 0.0 68-69 0.7625 0.0 0.0 0.0 0.0 70-71 1.025 0.0 0.0 0.0 0.0 72-73 1.2875 0.0 0.0 0.0 0.0 74-75 1.6875 0.0 0.0 0.0 0.0 76-77 2.0250000000000004 0.0 0.0 0.0 0.0 78-79 2.4375 0.0 0.0 0.0 0.0 80-81 2.8125 0.0 0.0 0.0 0.0 82-83 3.2625 0.0 0.0 0.0 0.0 84-85 3.925 0.0 0.0 0.0 0.0 86-87 4.6625 0.0 0.0 0.0 0.0 88-89 5.7 0.0 0.0 0.0 0.0 90-91 6.4625 0.0 0.0 0.0 0.0 92-93 7.2 0.0 0.0 0.0 0.0 94-95 8.0 0.0 0.0 0.0 0.0 96-97 9.0125 0.0 0.0 0.0 0.0 98-99 9.95 0.0 0.0 0.0 0.0 100-101 10.925 0.0 0.0 0.0 0.0 102-103 11.9875 0.0 0.0 0.0 0.0 104-105 12.9375 0.0 0.0 0.0 0.0 106-107 13.8875 0.0 0.0 0.0 0.0 108-109 14.125 0.0 0.0 0.0 0.0 110-111 14.125 0.0 0.0 0.0 0.0 112-113 14.125 0.0 0.0 0.0 0.0 114-115 14.125 0.0 0.0 0.0 0.0 116-117 14.125 0.0 0.0 0.0 0.0 118-119 14.125 0.0 0.0 0.0 0.0 120-121 14.125 0.0 0.0 0.0 0.0 122-123 14.125 0.0 0.0 0.0 0.0 124-125 14.125 0.0 0.0 0.0 0.0 126-127 14.125 0.0 0.0 0.0 0.0 128-129 14.125 0.0 0.0 0.0 0.0 130-131 14.125 0.0 0.0 0.0 0.0 132-133 14.125 0.0 0.0 0.0 0.0 134-135 14.125 0.0 0.0 0.0 0.0 136-137 14.125 0.0 0.0 0.0 0.0 138-139 14.125 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content pass >>END_MODULE Read 1346680 spots for SRR6941552.sra Written 1346680 spots for SRR6941552.sra Read 1346680 spots for SRR6941552.sra Written 1346680 spots for SRR6941552.sra Read 1346680 spots for SRR6941552.sra Written 1346680 spots for SRR6941552.sra Read 1346680 spots for SRR6941552.sra Written 1346680 spots for SRR6941552.sra Read 1346680 spots for SRR6941552.sra Written 1346680 spots for SRR6941552.sra Read 1346680 spots for SRR6941552.sra Written 1346680 spots for SRR6941552.sra Read 1346680 spots for SRR6941552.sra Written 1346680 spots for SRR6941552.sra Read 1346680 spots for SRR6941552.sra Written 1346680 spots for SRR6941552.sra Read 1346680 spots for SRR6941552.sra Written 1346680 spots for SRR6941552.sra Read 1346680 spots for SRR6941552.sra Written 1346680 spots for SRR6941552.sra Read 1346680 spots for SRR6941552.sra Written 1346680 spots for SRR6941552.sra Read 1346680 spots for SRR6941552.sra Written 1346680 spots for SRR6941552.sra Read 1346680 spots for SRR6941552.sra Written 1346680 spots for SRR6941552.sra Read 1346680 spots for SRR6941552.sra Written 1346680 spots for SRR6941552.sra Read 1346680 spots for SRR6941552.sra Written 1346680 spots for SRR6941552.sra Read 1346680 spots for SRR6941552.sra Written 1346680 spots for SRR6941552.sra Read 1346680 spots for SRR6941552.sra Written 1346680 spots for SRR6941552.sra Read 1346681 spots for SRR6941552.sra Written 1346681 spots for SRR6941552.sra Read 1346680 spots for SRR6941552.sra Written 1346680 spots for SRR6941552.sra Read 1346680 spots for SRR6941552.sra Written 1346680 spots for SRR6941552.sra SRR ids: ['SRR6941552.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_nzzofpjg SRR6941552.sra spots: 26933601 blocks: [[1, 1346680], [1346681, 2693360], [2693361, 4040040], [4040041, 5386720], [5386721, 6733400], [6733401, 8080080], [8080081, 9426760], [9426761, 10773440], [10773441, 12120120], [12120121, 13466800], [13466801, 14813480], [14813481, 16160160], [16160161, 17506840], [17506841, 18853520], [18853521, 20200200], [20200201, 21546880], [21546881, 22893560], [22893561, 24240240], [24240241, 25586920], [25586921, 26933601]] SRR6941552 file size 9105213 SRR6941552 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR6941552 SRR6941552_1.fastq SRR6941552_2.fastq Input file: SRR6941552_1.fastq Paired file: SRR6941552_2.fastq trimmed: SRR6941552-trimmed-pair1.fastq, SRR6941552-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Fri Dec 6 11:06:06 2024 >> started Fri Dec 6 11:06:37 2024 >> done (31.080s) 26933601 read pairs processed; of these: 28513 ( 0.11%) short read pairs filtered out after trimming by size control 25542 ( 0.09%) empty read pairs filtered out after trimming by size control 26879546 (99.80%) read pairs available; of these: 23813163 (88.59%) trimmed read pairs available after processing 3066383 (11.41%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 20 0.00% 19 23 0.00% 20 26 0.00% 21 33 0.00% 22 45 0.00% 23 48 0.00% 24 74 0.00% 25 70 0.00% 26 131 0.00% 27 162 0.00% 28 222 0.00% 29 326 0.00% 30 410 0.00% 31 444 0.00% 32 578 0.00% 33 664 0.00% 34 758 0.00% 35 965 0.00% 36 910 0.00% 37 973 0.00% 38 1098 0.00% 39 1540 0.01% 40 1690 0.01% 41 1979 0.01% 42 1942 0.01% 43 2097 0.01% 44 2468 0.01% 45 2599 0.01% 46 2812 0.01% 47 3014 0.01% 48 3545 0.01% 49 4140 0.02% 50 4302 0.02% 51 4953 0.02% 52 5834 0.02% 53 6294 0.02% 54 7355 0.03% 55 7077 0.03% 56 7726 0.03% 57 8763 0.03% 58 10856 0.04% 59 11349 0.04% 60 11669 0.04% 61 15666 0.06% 62 17912 0.07% 63 17268 0.06% 64 18771 0.07% 65 20984 0.08% 66 21578 0.08% 67 23255 0.09% 68 27951 0.10% 69 33260 0.12% 70 35080 0.13% 71 34885 0.13% 72 44242 0.16% 73 48775 0.18% 74 45323 0.17% 75 48894 0.18% 76 54200 0.20% 77 62431 0.23% 78 58035 0.22% 79 67968 0.25% 80 71565 0.27% 81 76775 0.29% 82 80440 0.30% 83 85180 0.32% 84 89742 0.33% 85 107538 0.40% 86 119599 0.44% 87 116406 0.43% 88 126592 0.47% 89 113995 0.42% 90 113791 0.42% 91 125257 0.47% 92 129113 0.48% 93 142446 0.53% 94 143550 0.53% 95 133488 0.50% 96 132439 0.49% 97 137825 0.51% 98 134133 0.50% 99 136045 0.51% 100 137685 0.51% 101 144243 0.54% 102 149981 0.56% 103 147271 0.55% 104 157708 0.59% 105 153372 0.57% 106 143758 0.53% 107 152569 0.57% 108 151663 0.56% 109 185922 0.69% 110 156346 0.58% 111 170169 0.63% 112 200633 0.75% 113 145770 0.54% 114 172699 0.64% 115 168314 0.63% 116 208528 0.78% 117 206918 0.77% 118 195045 0.73% 119 224956 0.84% 120 239003 0.89% 121 226764 0.84% 122 218794 0.81% 123 238825 0.89% 124 236321 0.88% 125 244383 0.91% 126 240185 0.89% 127 252870 0.94% 128 268547 1.00% 129 330369 1.23% 130 503545 1.87% 131 914145 3.40% 132 708865 2.64% 133 3097168 11.52% 134 3781064 14.07% 135 123098 0.46% 136 108469 0.40% 137 109927 0.41% 138 120717 0.45% 139 154631 0.58% 140 539359 2.01% 141 1204769 4.48% 142 242219 0.90% 143 1447083 5.38% 144 129194 0.48% 145 449166 1.67% 146 55288 0.21% 147 65679 0.24% 148 75453 0.28% 149 114582 0.43% 150 864750 3.22% 151 3066383 11.41% 26879546 reads passed initial QC criterion=sequence-density sequence-density=4.33 sequence-density-rank=1 fanout-score=2.01 fanout-score-rank=27 prefix-density=4.28 prefix-fanout=2.0 sequence=GTATTTAGCCTTG criterion=fanout-score sequence-density=0.07 sequence-density-rank=25 fanout-score=67.18 fanout-score-rank=1 prefix-density=4.44 prefix-fanout=1.0 sequence=GCTGATCATCCGAAAAGACCAGCTAAGCATCATTGGCTTGGTCAGCCTTTACCTGACCAACTACCTAATACTACGCAGGCTCATCAAACAGCGCTTTTGAGCTTTCTTCAGGATTTGGCCCGAACTGTTCGGCAGATTCCCACGCGTTACGCACCCGTTCGC criterion=sequence-density sequence-density=1.03 sequence-density-rank=1 fanout-score=7.05 fanout-score-rank=13 prefix-density=4.82 prefix-fanout=1.5 sequence=TGGTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGTTAACGAACGAGACCTCAGCCTGCTAACTAGCTATGCGGAGCCATCCCTCCGCAGCTAGCTTCTTAGAGGGACTATCGCCGTTTAGGCGACGGAAGTTTGAGGCAATAACAGGTCTGTGATGCCCTTAGATGTTCTGGGCCGCACGCGCGCTACACTGATGTATTCAACGAGTATATAGCCTTGGCCGACAGGCCCGGGTAATCTTGGGAAATTTCATCGTGATGGGGATAGATCATTGCAATTGTTGGTCTTCAACGAGGAATGCCTAGTAAGCGCGAGTCATCAGCTCGCGTTGACTACGTCCCTGCCCTTTGTACACACCGCCCGTC criterion=fanout-score sequence-density=0.03 sequence-density-rank=31 fanout-score=118.56 fanout-score-rank=1 prefix-density=3.25 prefix-fanout=1.0 sequence=CGAACGGGTGCGTAACGCGTGGGAATCTGCCGAACAGTTCGGGCCAAATCCTGAAGAAAGCTCAAAAGCGCTGTTTGATGAGCCTGCGTAGTATTAGGTAGTTGGTCAGGTAAAGGCTGACCAAGCCAATGATGCTTAGCTGGTCTTTTCGGATGATCAGC Potential 3prime adapter identified. Now checking if in reference sequence /dee2/code/volunteer_pipeline.sh: line 905: -f: command not found /dee2/code/volunteer_pipeline.sh: line 906: -f: command not found Adapter seq not found in reference. Now shuffling file before clipping skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -t 20 -x GTATTTAGCCTTG -y TGGTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGTTAACGAACGAGACCTCAGCCTGCTAACTAGCTATGCGGAGCCATCCCTCCGCAGCTAGCTTCTTAGAGGGACTATCGCCGTTTAGGCGACGGAAGTTTGAGGCAATAACAGGTCTGTGATGCCCTTAGATGTTCTGGGCCGCACGCGCGCTACACTGATGTATTCAACGAGTATATAGCCTTGGCCGACAGGCCCGGGTAATCTTGGGAAATTTCATCGTGATGGGGATAGATCATTGCAATTGTTGGTCTTCAACGAGGAATGCCTAGTAAGCGCGAGTCATCAGCTCGCGTTGACTACGTCCCTGCCCTTTGTACACACCGCCCGTC -o SRR6941552 SRR6941552_1.fastq SRR6941552_2.fastq Input file: SRR6941552_1.fastq Paired file: SRR6941552_2.fastq trimmed: SRR6941552-trimmed-pair1.fastq, SRR6941552-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): GTATTTAGCCTTG -- paired 3' end adapter sequence (-y): TGGTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGTTAACGAACGAGAC -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Fri Dec 6 11:08:27 2024 >> started Fri Dec 6 11:08:42 2024 >> done (15.213s) 13439773 read pairs processed; of these: 863 ( 0.01%) short read pairs filtered out after trimming by size control 1378 ( 0.01%) empty read pairs filtered out after trimming by size control 13437532 (99.98%) read pairs available; of these: 19845 ( 0.15%) trimmed read pairs available after processing 13417687 (99.85%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 9 0.00% 19 16 0.00% 20 14 0.00% 21 19 0.00% 22 23 0.00% 23 24 0.00% 24 29 0.00% 25 37 0.00% 26 62 0.00% 27 72 0.00% 28 115 0.00% 29 152 0.00% 30 184 0.00% 31 235 0.00% 32 312 0.00% 33 329 0.00% 34 388 0.00% 35 468 0.00% 36 459 0.00% 37 475 0.00% 38 537 0.00% 39 759 0.01% 40 814 0.01% 41 929 0.01% 42 965 0.01% 43 1076 0.01% 44 1268 0.01% 45 1285 0.01% 46 1387 0.01% 47 1475 0.01% 48 1771 0.01% 49 2050 0.02% 50 2145 0.02% 51 2437 0.02% 52 2905 0.02% 53 3146 0.02% 54 3643 0.03% 55 3551 0.03% 56 3843 0.03% 57 4386 0.03% 58 5428 0.04% 59 5755 0.04% 60 5895 0.04% 61 7877 0.06% 62 8940 0.07% 63 8715 0.06% 64 9336 0.07% 65 10552 0.08% 66 10787 0.08% 67 11651 0.09% 68 14012 0.10% 69 16686 0.12% 70 17673 0.13% 71 17552 0.13% 72 22356 0.17% 73 24428 0.18% 74 22412 0.17% 75 24527 0.18% 76 27292 0.20% 77 31282 0.23% 78 28951 0.22% 79 34331 0.26% 80 35600 0.26% 81 38536 0.29% 82 40052 0.30% 83 42718 0.32% 84 44876 0.33% 85 53735 0.40% 86 59626 0.44% 87 57914 0.43% 88 63236 0.47% 89 57017 0.42% 90 56773 0.42% 91 62613 0.47% 92 64663 0.48% 93 71162 0.53% 94 71778 0.53% 95 66950 0.50% 96 66388 0.49% 97 68721 0.51% 98 67021 0.50% 99 67961 0.51% 100 69048 0.51% 101 72128 0.54% 102 75172 0.56% 103 73524 0.55% 104 78788 0.59% 105 76736 0.57% 106 72048 0.54% 107 76148 0.57% 108 76405 0.57% 109 93357 0.69% 110 77984 0.58% 111 85355 0.64% 112 100841 0.75% 113 72120 0.54% 114 86547 0.64% 115 83808 0.62% 116 108875 0.81% 117 99876 0.74% 118 99777 0.74% 119 108410 0.81% 120 118768 0.88% 121 113029 0.84% 122 109560 0.82% 123 119528 0.89% 124 118207 0.88% 125 122165 0.91% 126 119971 0.89% 127 126433 0.94% 128 134217 1.00% 129 165590 1.23% 130 252474 1.88% 131 457875 3.41% 132 353403 2.63% 133 1547902 11.52% 134 1890544 14.07% 135 63280 0.47% 136 52318 0.39% 137 54801 0.41% 138 60421 0.45% 139 78178 0.58% 140 268724 2.00% 141 602276 4.48% 142 121288 0.90% 143 723749 5.39% 144 64504 0.48% 145 224357 1.67% 146 27538 0.20% 147 32927 0.25% 148 37862 0.28% 149 57160 0.43% 150 431306 3.21% 151 1532658 11.41% criterion=sequence-density sequence-density=4.31 sequence-density-rank=1 fanout-score=2.00 fanout-score-rank=27 prefix-density=4.29 prefix-fanout=2.0 sequence=GTATTTAGCCTTG criterion=fanout-score sequence-density=0.06 sequence-density-rank=30 fanout-score=70.16 fanout-score-rank=1 prefix-density=4.43 prefix-fanout=1.0 sequence=GCTGATCATCCGAAAAGACCAGCTAAGCATCATTGGCTTGGTCAGCCTTTACCTGACCAACTACCTAATACTACGCAGGCTCATCAAACAGCGCTTTTGAGCTTTCTTCAGGATTTGGCCCGAACTGTTCGGCAGATTCCCACGCGTTACGCACCCGTTCGC criterion=sequence-density sequence-density=1.18 sequence-density-rank=1 fanout-score=1.97 fanout-score-rank=35 prefix-density=1.19 prefix-fanout=2.0 sequence=CCTAGTACGAGAGGA criterion=fanout-score sequence-density=0.03 sequence-density-rank=33 fanout-score=124.81 fanout-score-rank=1 prefix-density=3.28 prefix-fanout=1.0 sequence=CGAACGGGTGCGTAACGCGTGGGAATCTGCCGAACAGTTCGGGCCAAATCCTGAAGAAAGCTCAAAAGCGCTGTTTGATGAGCCTGCGTAGTATTAGGTAGTTGGTCAGGTAAAGGCTGACCAAGCCAATGATGCTTAGCTGGTCTTTTCGGATGATCAGC SRR6941552 testing PE reads STAR mapping to Ensembl genome Started job on | Dec 06 11:09:34 Started mapping on | Dec 06 11:09:34 Finished on | Dec 06 11:11:31 Mapping speed, Million of reads per hour | 826.99 Number of input reads | 26877305 Average input read length | 256 UNIQUE READS: Uniquely mapped reads number | 7040941 Uniquely mapped reads % | 26.20% Average mapped length | 267.04 Number of splices: Total | 978366 Number of splices: Annotated (sjdb) | 607075 Number of splices: GT/AG | 667151 Number of splices: GC/AG | 13028 Number of splices: AT/AC | 3390 Number of splices: Non-canonical | 294797 Mismatch rate per base, % | 0.27% Deletion rate per base | 0.00% Deletion average length | 1.71 Insertion rate per base | 0.00% Insertion average length | 1.80 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 11578211 % of reads mapped to multiple loci | 43.08% Number of reads mapped to too many loci | 1488101 % of reads mapped to too many loci | 5.54% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 4.12% % of reads unmapped: other | 21.07% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 8268369 8268369 8268369 N_multimapping 11578211 11578211 11578211 N_noFeature 5358160 6973212 5392294 N_ambiguous 90495 2157 56648 UnstrandedReadsAssigned:1592286 PositiveStrandReadsAssigned:65572 NegativeStrandReadsAssigned:1591999 Dataset is classified negative stranded MeadianReadLen=151 20thPercentileLength=114 echo kmer=109 SRR6941552 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,972 [index] number of k-mers: 66,720,672 [index] number of equivalence classes: 111,837 [quant] running in paired-end mode [quant] will process pair 1: SRR6941552-trimmed-pair1.fastq SRR6941552-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 26,877,305 reads, 5,232,295 reads pseudoaligned [quant] estimated average fragment length: 163.403 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 922 rounds 52973 SRR6941552.ke.tsv 35125 SRR6941552.se.tsv 88098 total ==> SRR6941552.ke.tsv <== target_id length eff_length est_counts tpm PNS24245 936 773.756 0 0 PNS24247 1044 881.597 1.2823 0.0880747 PNS24249 1928 1765.6 13.1531 0.451096 PNS24246 1044 881.597 1.2823 0.0880747 PNS24248 1044 881.597 1.2823 0.0880747 PNS24244 1471 1308.6 0 0 PNS24243 293 134.171 0 0 KQK14069 1603 1440.6 386.571 16.2487 KQK14071 474 312.263 84.004 16.2896 ==> SRR6941552.se.tsv <== BRADI_1g14170v3 591 BRADI_1g53295v3 4 BRADI_1g59795v3 6 BRADI_1g07683v3 0 BRADI_1g00485v3 0 BRADI_1g20270v3 19 BRADI_1g74790v3 9 BRADI_1g09890v3 0 BRADI_1g77505v3 5 BRADI_1g48960v3 0 SRR6941552 completed mapping pipeline successfully