Starting /dee2/code/volunteer_pipeline.sh SRR6941553 current disk space = 1516105830400 free memory = 1596731332 SRR6941553 SRAfilesize afb21255f46bd9dc992042973fdcdb26 SRR6941553.sra SRR6941553.sra file validated SRR6941553 is paired end SRR6941553 is conventional basespace SRR6941553 read1 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR6941553_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 34.5845 35.0 35.0 35.0 35.0 35.0 2 34.5825 35.0 35.0 35.0 34.0 35.0 3 34.578 35.0 35.0 35.0 34.0 35.0 4 34.56825 35.0 35.0 35.0 34.0 35.0 5 34.57625 35.0 35.0 35.0 34.0 35.0 6 39.35825 40.0 40.0 40.0 39.0 40.0 7 39.4755 40.0 40.0 40.0 39.0 40.0 8 39.16625 40.0 40.0 40.0 39.0 40.0 9 39.38825 40.0 40.0 40.0 39.0 40.0 10-14 39.36785 40.0 40.0 40.0 39.0 40.0 15-19 39.40995 40.0 40.0 40.0 39.0 40.0 20-24 39.299 40.0 40.0 40.0 38.8 40.0 25-29 39.3018 40.0 40.0 40.0 39.0 40.0 30-34 39.32965 40.0 40.0 40.0 39.0 40.0 35-39 39.3023 40.0 40.0 40.0 39.0 40.0 40-44 39.31015000000001 40.0 40.0 40.0 39.0 40.0 45-49 39.314 40.0 40.0 40.0 39.0 40.0 50-54 39.30705 40.0 40.0 40.0 39.0 40.0 55-59 39.20765 40.0 40.0 40.0 38.8 40.0 60-64 39.24985 40.0 40.0 40.0 39.0 40.0 65-69 39.252250000000004 40.0 40.0 40.0 39.0 40.0 70-74 39.23265 40.0 40.0 40.0 39.0 40.0 75-79 39.22385 40.0 40.0 40.0 38.8 40.0 80-84 39.145050000000005 40.0 40.0 40.0 38.6 40.0 85-89 39.17695 40.0 40.0 40.0 39.0 40.0 90-94 39.18345000000001 40.0 40.0 40.0 39.0 40.0 95-99 39.01965 40.0 39.8 40.0 38.0 40.0 100-104 38.2966 39.4 38.6 39.6 36.8 39.8 105-109 38.990950000000005 40.0 39.6 40.0 37.6 40.0 110-114 39.14135 40.0 40.0 40.0 38.4 40.0 115-119 39.158 40.0 40.0 40.0 38.8 40.0 120-124 39.103500000000004 40.0 40.0 40.0 38.2 40.0 125-129 39.04385 40.0 40.0 40.0 38.4 40.0 130-134 38.9931 40.0 39.8 40.0 37.8 40.0 135-139 38.812799999999996 40.0 39.0 40.0 37.2 40.0 140-144 38.818549999999995 40.0 39.0 40.0 37.4 40.0 145-149 38.706149999999994 40.0 39.2 40.0 37.2 40.0 150-151 37.337 39.5 37.5 40.0 33.5 40.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 23 1.0 24 4.0 25 1.0 26 8.0 27 9.0 28 8.0 29 17.0 30 26.0 31 29.0 32 41.0 33 44.0 34 51.0 35 59.0 36 94.0 37 123.0 38 254.0 39 3231.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 53.5140562248996 12.173694779116467 5.371485943775101 28.940763052208833 2 23.45 14.674999999999999 32.425 29.45 3 21.65 22.400000000000002 26.575 29.375 4 25.324999999999996 29.549999999999997 21.55 23.575 5 22.5 35.075 23.175 19.25 6 18.475 38.9 22.400000000000002 20.225 7 13.475000000000001 30.15 39.85 16.525000000000002 8 16.325 27.450000000000003 31.125000000000004 25.1 9 15.950000000000001 26.224999999999998 33.35 24.474999999999998 10-14 19.35 33.82 25.39 21.44 15-19 20.125 31.505 25.480000000000004 22.89 20-24 18.941894189418942 31.863186318631865 26.552655265526553 22.642264226422643 25-29 22.045 31.895 25.615 20.445 30-34 22.105 33.379999999999995 23.895 20.62 35-39 20.94 31.369999999999997 26.255 21.435000000000002 40-44 19.112645058023208 30.972388955582232 26.625650260104038 23.289315726290518 45-49 19.794999999999998 30.73 27.565 21.91 50-54 21.529999999999998 30.86 25.605 22.005 55-59 20.225 30.570000000000004 26.39 22.814999999999998 60-64 18.785 30.94 27.065 23.21 65-69 19.915 31.59 25.645 22.85 70-74 20.419999999999998 31.45 24.505 23.625 75-79 20.84 29.625 26.505000000000003 23.03 80-84 22.14 29.794999999999998 25.619999999999997 22.445 85-89 21.355 29.830000000000002 26.665 22.15 90-94 18.886888688868886 31.983198319831985 25.862586258625864 23.26732673267327 95-99 19.91 31.805 24.11 24.175 100-104 20.064999999999998 31.669999999999998 25.295 22.97 105-109 19.785 31.555 26.02 22.64 110-114 21.235 29.64 25.650000000000002 23.474999999999998 115-119 19.830000000000002 31.790000000000003 24.33 24.05 120-124 19.735 31.130000000000003 24.005000000000003 25.130000000000003 125-129 19.650000000000002 31.330000000000002 25.025 23.995 130-134 21.015 32.49 23.76 22.735 135-139 21.995 31.295 24.665 22.045 140-144 22.275 30.805 25.124999999999996 21.795 145-149 19.915 31.185000000000002 24.825 24.075 150-151 19.5 33.125 23.5125 23.8625 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.5 10 0.5 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.5 19 1.5 20 3.0 21 3.0 22 4.0 23 5.0 24 2.5 25 2.0 26 6.5 27 11.0 28 11.5 29 13.5 30 23.0 31 30.5 32 35.5 33 36.5 34 37.5 35 44.0 36 104.5 37 229.0 38 273.0 39 265.5 40 290.0 41 322.0 42 269.0 43 232.5 44 236.0 45 215.0 46 186.5 47 150.0 48 135.0 49 94.0 50 79.5 51 69.5 52 48.0 53 48.5 54 55.0 55 65.0 56 64.5 57 49.0 58 44.5 59 42.5 60 36.0 61 28.5 62 20.0 63 15.0 64 17.5 65 18.5 66 10.0 67 4.0 68 3.5 69 1.5 70 0.5 71 1.0 72 2.5 73 1.5 74 0.0 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.4 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.01 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.04 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.01 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.1 #Duplication Level Percentage of deduplicated Percentage of total 1 83.53462157809984 51.87500000000001 2 8.252818035426731 10.25 3 2.737520128824477 5.1 4 1.3687600644122384 3.4000000000000004 5 0.8051529790660225 2.5 6 0.644122383252818 2.4 7 0.6038647342995169 2.625 8 0.44283413848631237 2.1999999999999997 9 0.08051529790660225 0.44999999999999996 >10 1.4492753623188406 16.225 >50 0.08051529790660225 2.9749999999999996 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GCGGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTACG 64 1.6 No Hit GGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTACGTT 55 1.375 No Hit CTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCATTAAT 43 1.075 No Hit GCGGAAAAACCTTATCCATTAATAGCGGGAACTTCAAGAGCAGCTAGATC 34 0.8500000000000001 No Hit GTGCTCTGCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTCCTA 33 0.8250000000000001 No Hit GCTTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCA 30 0.75 No Hit GCCAAAATAACCATGAGCAGCCACAATATTATAAGTCTCTTCCTCTTGAC 26 0.65 No Hit GTTGAAACTAGCATATTGGAAGATTAATCGGCCAAAATAACCATGAGCAG 26 0.65 No Hit GCTCTGCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTCCTAAA 24 0.6 No Hit GGCCAAAATAACCATGAGCAGCCACAATATTATAAGTCTCTTCCTCTTGA 22 0.5499999999999999 No Hit GTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTC 21 0.525 No Hit GGAAAAACCTTATCCATTAATAGCGGGAACTTCAAGAGCAGCTAGATCTA 21 0.525 No Hit GCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTCCTAAAGGCAT 21 0.525 No Hit GGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTACGTTC 21 0.525 No Hit CTTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCAT 20 0.5 No Hit GCGACCTTGACTATCAACTACAGATTGGTTGAAATTGAATCCATTTAGGT 19 0.475 No Hit GTTGATGATATCAGCCCAAGTATTAATAACGCGACCTTGACTATCAACTA 18 0.44999999999999996 No Hit CGGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTACGT 17 0.42500000000000004 No Hit GTTAGCGGAAAAACCTTATCCATTAATAGCGGGAACTTCAAGAGCAGCTA 17 0.42500000000000004 No Hit CCACAATATTATAAGTCTCTTCCTCTTGACCAAATTTGTAACCCTCATTA 15 0.375 No Hit CTCATAAGGACCACCATTGTATAACCATTCATCAACGGATGCAGCTTCCC 15 0.375 No Hit CTCTGCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTCCTAAAG 15 0.375 No Hit GCACGGTTGATGATATCAGCCCAAGTATTAATAACGCGACCTTGACTATC 14 0.35000000000000003 No Hit CCAGATTCCTACTACAGGCCAAGCAGCCAAGAAGAAGTGTAAAGAACGAG 14 0.35000000000000003 No Hit GCATAAGGATGTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAAAGTA 14 0.35000000000000003 No Hit GTTGAAATTGAATCCATTTAGGTTGAACGCCATAGTACTAATACCTAAAG 14 0.35000000000000003 No Hit CCCAAGTATTAATAACGCGACCTTGACTATCAACTACAGATTGGTTGAAA 13 0.325 No Hit CTCATTAGCAGATTCATTTTCAGTAGTTTCCCTGATCAAACTAGAGGTTA 13 0.325 No Hit GTGAGCATTACGTTCGTGCATTACTTCCATACCAAGATTAGCACGGTTGA 12 0.3 No Hit GGATGCATAAGGATGTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAA 12 0.3 No Hit GGAGGGGCTGCGATGAAGGCGATAATAAATACAGAAGTTGCGGTCAATAA 11 0.27499999999999997 No Hit GCCGAAAACACCAGCTACACCTAACATGTGAAATGGATGCATAAGGATGT 11 0.27499999999999997 No Hit GCATATTGGAAGATTAATCGGCCAAAATAACCATGAGCAGCCACAATATT 11 0.27499999999999997 No Hit TTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCATTAATA 11 0.27499999999999997 No Hit GCCCAAGTATTAATAACGCGACCTTGACTATCAACTACAGATTGGTTGAA 11 0.27499999999999997 No Hit CATAAGGATGTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAAAGTAC 10 0.25 No Hit GGGGCTGCGATGAAGGCGATAATAAATACAGAAGTTGCGGTCAATAAGGT 10 0.25 No Hit GCCAAGAAGAAGTGTAAAGAACGAGAGTTGTTGAAACTAGCATATTGGAA 10 0.25 No Hit GGTCAATAAGGTAGGGATCATCAAAACACCGAACCATCCGATGTAAAGAC 9 0.22499999999999998 No Hit GGCCAAGCAGCCAAGAAGAAGTGTAAAGAACGAGAGTTGTTGAAACTAGC 9 0.22499999999999998 No Hit GCGGTCAATAAGGTAGGGATCATCAAAACACCGAACCATCCGATGTAAAG 8 0.2 No Hit GGCGCATACCCAAACGGAAACTAAGTTCCCACTCACGACCCATATAACAA 8 0.2 No Hit GGGAAGTTGTGAGCATTACGTTCGTGCATTACTTCCATACCAAGATTAGC 8 0.2 No Hit GCTGCGATGAAGGCGATAATAAATACAGAAGTTGCGGTCAATAAGGTAGG 8 0.2 No Hit GGCCCATATAACAAGCTACACCAAGTAAGAAGTGTAGAACAATTAGCTCA 8 0.2 No Hit GATGCATAAGGATGTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAAA 8 0.2 No Hit GAACAATTAGCTCATAAGGACCACCATTGTATAACCATTCATCAACGGAT 8 0.2 No Hit GCTAGATCTAGAGGGAAGTTGTGAGCATTACGTTCGTGCATTACTTCCAT 8 0.2 No Hit CTCTTGACCAAATTTGTAACCCTCATTAGCAGATTCATTTTCAGTAGTTT 8 0.2 No Hit GGAATAATAGCACCAGAGATAATATTGTTTCCATAAAGTAAAGAACCAGA 8 0.2 No Hit CGCGACCTTGACTATCAACTACAGATTGGTTGAAATTGAATCCATTTAGG 8 0.2 No Hit GTGCAATCCGATTGCCGCAGAAGTAGGAATAATAGCACCAGAGATAATAT 7 0.17500000000000002 No Hit CCCATATAACAAGCTACACCAAGTAAGAAGTGTAGAACAATTAGCTCATA 7 0.17500000000000002 No Hit GGAAGATTAATCGGCCAAAATAACCATGAGCAGCCACAATATTATAAGTC 7 0.17500000000000002 No Hit CTGCGATGAAGGCGATAATAAATACAGAAGTTGCGGTCAATAAGGTAGGG 7 0.17500000000000002 No Hit CAATATTATAAGTCTCTTCCTCTTGACCAAATTTGTAACCCTCATTAGCA 7 0.17500000000000002 No Hit ACATGCTCCACCGCTTGTGCGGGCCCCCGTCAATTCCTTTGAGTTTCATT 7 0.17500000000000002 No Hit GCTAGTTATCCAGTTGCAGAAGCGACCCCACAGGCTTGTACTTTCGCGTC 7 0.17500000000000002 No Hit CATATAACAAGCTACACCAAGTAAGAAGTGTAGAACAATTAGCTCATAAG 7 0.17500000000000002 No Hit GGATGTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAAAGTACCAGAT 7 0.17500000000000002 No Hit GTTCGCTGGAGTAGGCTGCGAGGCCCAAGCGGCCAAGGAGTGCAGCGGCC 7 0.17500000000000002 No Hit GAAAAACCTTATCCATTAATAGCGGGAACTTCAAGAGCAGCTAGATCTAG 7 0.17500000000000002 No Hit AGCAGATTCATTTTCAGTAGTTTCCCTGATCAAACTAGAGGTTACCAAGG 7 0.17500000000000002 No Hit GGTAAATCAAGAAAACAGCAGTCGCAGCTGCAACAGGAGCTGAATATGCA 7 0.17500000000000002 No Hit GTCAATAAGGTAGGGATCATCAAAACACCGAACCATCCGATGTAAAGACG 7 0.17500000000000002 No Hit ATAACAAGCTACACCAAGTAAGAAGTGTAGAACAATTAGCTCATAAGGAC 7 0.17500000000000002 No Hit GGTTGATGATATCAGCCCAAGTATTAATAACGCGACCTTGACTATCAACT 6 0.15 No Hit GGTAAAAGTGCAATCCGATTGCCGCAGAAGTAGGAATAATAGCACCAGAG 6 0.15 No Hit AACGAGAGTTGTTGAAACTAGCATATTGGAAGATTAATCGGCCAAAATAA 6 0.15 No Hit CCAAGAAGAAGTGTAAAGAACGAGAGTTGTTGAAACTAGCATATTGGAAG 6 0.15 No Hit GCATTACGTTCGTGCATTACTTCCATACCAAGATTAGCACGGTTGATGAT 6 0.15 No Hit TTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCATTA 6 0.15 No Hit CTAACATGTGAAATGGATGCATAAGGATGTTGTGCTCTGCCTGGAATACA 6 0.15 No Hit CATCAGAGAAGCTTCCTTGACCAATAGGGTAAATCAAGAAAACAGCAGTC 6 0.15 No Hit GTAAAGAACGAGAGTTGTTGAAACTAGCATATTGGAAGATTAATCGGCCA 6 0.15 No Hit GTAACCCTCATTAGCAGATTCATTTTCAGTAGTTTCCCTGATCAAACTAG 6 0.15 No Hit GAAGATTAATCGGCCAAAATAACCATGAGCAGCCACAATATTATAAGTCT 6 0.15 No Hit GAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTACGTTCG 6 0.15 No Hit GTGAAATGGATGCATAAGGATGTTGTGCTCTGCCTGGAATACAATCATAA 6 0.15 No Hit GACCCATATAACAAGCTACACCAAGTAAGAAGTGTAGAACAATTAGCTCA 6 0.15 No Hit CTTATCCATTAATAGCGGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAG 6 0.15 No Hit CTCGCGAATACCATCAATATCTACTGGAGGGGCTGCGATGAAGGCGATAA 6 0.15 No Hit AGAGAAGCTTCCTTGACCAATAGGGTAAATCAAGAAAACAGCAGTCGCAG 5 0.125 No Hit CGCAGAAGTAGGAATAATAGCACCAGAGATAATATTGTTTCCATAAAGTA 5 0.125 No Hit CTTGACTATCAACTACAGATTGGTTGAAATTGAATCCATTTAGGTTGAAC 5 0.125 No Hit AGTGAACCAGATTCCTACTACAGGCCAAGCAGCCAAGAAGAAGTGTAAAG 5 0.125 No Hit CTACTACAGGCCAAGCAGCCAAGAAGAAGTGTAAAGAACGAGAGTTGTTG 5 0.125 No Hit GTCGCAGCTGCAACAGGAGCTGAATATGCAACAGCAATCCAAGGGCGCAT 5 0.125 No Hit GGCGATAATAAATACAGAAGTTGCGGTCAATAAGGTAGGGATCATCAAAA 5 0.125 No Hit GTCGGTTTCGGGTACAGGTACCCTTTTGTTGAAGGTCGTTCGAGCTTTTC 5 0.125 No Hit CATAGTACTAATACCTAAAGCAGTGAACCAGATTCCTACTACAGGCCAAG 5 0.125 No Hit ATAAGGACCACCATTGTATAACCATTCATCAACGGATGCAGCTTCCCAAA 5 0.125 No Hit GTTGCGGTCAATAAGGTAGGGATCATCAAAACACCGAACCATCCGATGTA 5 0.125 No Hit TCTTGACCAAATTTGTAACCCTCATTAGCAGATTCATTTTCAGTAGTTTC 5 0.125 No Hit ATAAGGATGTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAAAGTACC 5 0.125 No Hit GTCAATTAGAAGAATAAAGAAGAATTACTGCATTTCGTAACTTATTTTTT 5 0.125 No Hit GCTGGAGTAGGCTGCGAGGCCCAAGCGGCCAAGGAGTGCAGCGGCCCGTT 5 0.125 No Hit CACGGTTGATGATATCAGCCCAAGTATTAATAACGCGACCTTGACTATCA 5 0.125 No Hit TAAAGAACGAGAGTTGTTGAAACTAGCATATTGGAAGATTAATCGGCCAA 5 0.125 No Hit GTGCATTACTTCCATACCAAGATTAGCACGGTTGATGATATCAGCCCAAG 5 0.125 No Hit CTGGAATACAATCATAAAGTTGAAAGTACCAGATATTCCTAAAGGCATAC 5 0.125 No Hit GAGCATTACGTTCGTGCATTACTTCCATACCAAGATTAGCACGGTTGATG 5 0.125 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0125 0.0 0.0 0.0 0.0 50-51 0.025 0.0 0.0 0.0 0.0 52-53 0.025 0.0 0.0 0.0 0.0 54-55 0.025 0.0 0.0 0.0 0.0 56-57 0.025 0.0 0.0 0.0 0.0 58-59 0.025 0.0 0.0 0.0 0.0 60-61 0.025 0.0 0.0 0.0 0.0 62-63 0.025 0.0 0.0 0.0 0.0 64-65 0.025 0.0 0.0 0.0 0.0 66-67 0.025 0.0 0.0 0.0 0.0 68-69 0.025 0.0 0.0 0.0 0.0 70-71 0.025 0.0 0.0 0.0 0.0 72-73 0.075 0.0 0.0 0.0 0.0 74-75 0.075 0.0 0.0 0.0 0.0 76-77 0.075 0.0 0.0 0.0 0.0 78-79 0.1125 0.0 0.0 0.0 0.0 80-81 0.1375 0.0 0.0 0.0 0.0 82-83 0.175 0.0 0.0 0.0 0.0 84-85 0.1875 0.0 0.0 0.0 0.0 86-87 0.2625 0.0 0.0 0.0 0.0 88-89 0.4125 0.0 0.0 0.0 0.0 90-91 0.475 0.0 0.0 0.0 0.0 92-93 0.5875 0.0 0.0 0.0 0.0 94-95 0.7124999999999999 0.0 0.0 0.0 0.0 96-97 0.8125 0.0 0.0 0.0 0.0 98-99 0.8625 0.0 0.0 0.0 0.0 100-101 0.95 0.0 0.0 0.0 0.0 102-103 1.0875 0.0 0.0 0.0 0.0 104-105 1.3125 0.0 0.0 0.0 0.0 106-107 1.5875 0.0 0.0 0.0 0.0 108-109 1.775 0.0 0.0 0.0 0.0 110-111 1.9500000000000002 0.0 0.0 0.0 0.0 112-113 2.1 0.0 0.0 0.0 0.0 114-115 2.3875 0.0 0.0 0.0 0.0 116-117 2.5999999999999996 0.0 0.0 0.0 0.0 118-119 2.7125000000000004 0.0 0.0 0.0 0.0 120-121 2.925 0.0 0.0 0.0 0.0 122-123 3.3125 0.0 0.0 0.0 0.0 124-125 3.5250000000000004 0.0 0.0 0.0 0.0 126-127 3.8 0.0 0.0 0.0 0.0 128-129 4.175 0.0 0.0 0.0 0.0 130-131 4.5 0.0 0.0 0.0 0.0 132-133 4.9125 0.0 0.0 0.0 0.0 134-135 5.2125 0.0 0.0 0.0 0.0 136-137 5.6625 0.0 0.0 0.0 0.0 138-139 6.0625 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TTCTTTT 10 0.006830828 145.0 4 TTCTTCA 10 0.006830828 145.0 9 ATTGAAT 45 0.008957279 48.333332 145 GCTAGAT 60 0.004491891 14.500001 15-19 AGCTAGA 60 0.004491891 14.500001 15-19 AGAGCAG 60 0.004491891 14.500001 10-14 ATCTAGA 65 0.0076375785 13.384615 20-24 AGAGGGA 65 0.0076375785 13.384615 25-29 CCCAAGT 65 0.0076375785 13.384615 95-99 GATCTAG 65 0.0076375785 13.384615 20-24 GGTTGAT 85 0.0031733946 11.941176 80-84 TACCAAG 90 0.0048656333 11.277777 65-69 >>END_MODULE SRR6941553 read2 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR6941553_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 34.07525 35.0 35.0 35.0 33.0 35.0 2 33.9675 35.0 35.0 35.0 32.0 35.0 3 33.83225 35.0 35.0 35.0 31.0 35.0 4 34.12925 35.0 35.0 35.0 33.0 35.0 5 34.1725 35.0 35.0 35.0 33.0 35.0 6 38.595 40.0 40.0 40.0 37.0 40.0 7 38.924 40.0 40.0 40.0 38.0 40.0 8 38.855 40.0 40.0 40.0 38.0 40.0 9 38.954 40.0 40.0 40.0 39.0 40.0 10-14 38.7664 40.0 40.0 40.0 37.6 40.0 15-19 38.52995 40.0 39.8 40.0 36.4 40.0 20-24 38.8277 40.0 40.0 40.0 37.8 40.0 25-29 38.747249999999994 40.0 40.0 40.0 37.6 40.0 30-34 38.7805 40.0 40.0 40.0 37.8 40.0 35-39 38.71385 40.0 40.0 40.0 37.2 40.0 40-44 38.8709 40.0 40.0 40.0 38.0 40.0 45-49 38.6543 40.0 39.6 40.0 36.8 40.0 50-54 38.7136 40.0 40.0 40.0 37.2 40.0 55-59 38.67405 40.0 39.6 40.0 37.0 40.0 60-64 38.77585 40.0 40.0 40.0 37.8 40.0 65-69 38.63879999999999 40.0 39.8 40.0 37.0 40.0 70-74 38.418400000000005 40.0 39.0 40.0 35.8 40.0 75-79 38.6742 40.0 39.6 40.0 37.2 40.0 80-84 38.677499999999995 40.0 39.2 40.0 37.0 40.0 85-89 38.40865 40.0 39.0 40.0 36.0 40.0 90-94 38.4038 40.0 39.0 40.0 36.0 40.0 95-99 38.2025 40.0 39.0 40.0 35.4 40.0 100-104 37.07195 38.6 37.8 39.4 33.4 39.6 105-109 38.01795 40.0 39.0 40.0 34.8 40.0 110-114 37.95755 40.0 39.0 40.0 34.4 40.0 115-119 37.7875 40.0 39.0 40.0 34.0 40.0 120-124 37.791549999999994 40.0 39.0 40.0 34.6 40.0 125-129 37.49505 40.0 38.8 40.0 33.2 40.0 130-134 37.84395 40.0 39.0 40.0 34.8 40.0 135-139 37.5264 40.0 39.0 40.0 33.8 40.0 140-144 37.40375 40.0 39.0 40.0 34.0 40.0 145-149 36.7332 40.0 38.8 40.0 30.4 40.0 150-151 34.677 38.5 35.0 39.5 23.5 40.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 14.0 3 1.0 4 0.0 5 0.0 6 0.0 7 0.0 8 1.0 9 0.0 10 1.0 11 0.0 12 0.0 13 1.0 14 0.0 15 0.0 16 1.0 17 5.0 18 7.0 19 0.0 20 5.0 21 10.0 22 8.0 23 15.0 24 12.0 25 14.0 26 22.0 27 18.0 28 24.0 29 29.0 30 43.0 31 42.0 32 38.0 33 52.0 34 72.0 35 101.0 36 107.0 37 169.0 38 354.0 39 2834.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 48.248046382656916 21.527602722460298 9.654650869674818 20.569700025207965 2 23.988947500627983 21.878924893242903 36.87515699572972 17.256970610399396 3 19.589178356713425 25.100200400801604 39.97995991983968 15.33066132264529 4 23.252890899949723 31.121166415284062 27.40070387129211 18.225238813474107 5 26.028084252758276 32.422266800401204 24.82447342026078 16.72517552657974 6 19.83429575696711 33.86894300778307 28.14461461210143 18.15214662314838 7 17.607223476297968 20.817657386506145 43.46626536242789 18.108853774767997 8 21.172638436482085 24.730643948884993 31.245301929341018 22.851415685291908 9 22.92658481583563 19.96993234778251 35.42971686294162 21.67376597344024 10-14 23.503159161568547 26.34640457326246 31.255641359943837 18.894794905225154 15-19 23.246010237880157 24.72146943691659 31.83278129077587 20.19973903442738 20-24 24.02486714128146 24.862127744911263 31.83094354757846 19.282061566228816 25-29 23.325310870437225 25.882470918572004 30.956678700361014 19.83553951062976 30-34 23.747243936660652 24.61916215674484 32.63680096211666 18.99679294447785 35-39 24.187888510126328 24.83958291558051 31.201122919590933 19.771405654702225 40-44 23.490774167669475 25.436221419975936 31.187324508624148 19.88567990373045 45-49 22.547496115093487 26.171737931725904 31.239661135896533 20.041104817284076 50-54 22.83164544269528 25.238142986062368 31.635415622179885 20.29479594906247 55-59 22.48847002205735 26.654301183075997 30.223581311409664 20.633647483456986 60-64 22.333600641539693 24.764434643143545 32.06194867682438 20.840016038492383 65-69 23.2693368088626 25.71056193292897 31.279763396661487 19.740337861546944 70-74 23.872067375175458 25.61159013434931 30.835171445758974 19.68117104471626 75-79 23.84056154424668 24.983705189270495 31.035347204813235 20.14038606166959 80-84 23.31094627105052 24.087810745789895 33.72093023255814 18.880312750601444 85-89 24.195649994988475 25.2230129297384 30.60038087601483 19.980956199258294 90-94 23.49137931034483 25.13031275060144 30.828989574979953 20.549318364073777 95-99 23.03337007716204 25.04258943781942 30.899889768513876 21.02415071650466 100-104 23.43491139113409 24.790401124554446 31.397158491892164 20.377528992419297 105-109 24.77617945880696 24.69067498239614 31.138718438788853 19.394427120008046 110-114 22.895504374937143 25.16343155989138 31.68057930202152 20.260484763149954 115-119 24.084400904295403 25.290128108515447 30.73599598090932 19.88947500627983 120-124 23.533853511200604 25.572615152277876 30.244147998993203 20.649383337528317 125-129 23.263400923509337 26.32001606103192 29.612527604898613 20.804055410560128 130-134 23.513445589344055 26.081930133199293 30.052777079668257 20.351847197788388 135-139 23.318069742867205 25.16982841040608 31.8371660041262 19.67493584260051 140-144 24.59709911361805 25.684931506849317 30.29311039484287 19.424858984689767 145-149 23.8030577348622 25.53309193321263 31.055119694226512 19.608730637698653 150-151 23.262536131707932 24.531858740731433 32.04725399019731 20.15835113736333 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 7.0 1 4.0 2 0.5 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.5 11 0.5 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.5 19 1.0 20 1.5 21 2.5 22 2.5 23 2.5 24 5.5 25 9.5 26 12.5 27 11.5 28 13.0 29 24.5 30 33.5 31 33.5 32 37.0 33 55.0 34 73.5 35 75.0 36 102.0 37 174.5 38 229.5 39 245.0 40 261.5 41 287.0 42 250.5 43 244.0 44 258.5 45 205.5 46 193.0 47 164.5 48 118.5 49 93.5 50 78.0 51 75.5 52 55.0 53 48.5 54 63.5 55 69.5 56 61.0 57 52.0 58 44.0 59 44.0 60 37.5 61 32.0 62 38.5 63 27.0 64 10.5 65 7.0 66 5.5 67 6.5 68 7.0 69 3.0 70 0.5 71 1.0 72 1.0 73 0.5 74 0.5 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.8250000000000001 2 0.475 3 0.2 4 0.5499999999999999 5 0.3 6 0.42500000000000004 7 0.325 8 0.22499999999999998 9 0.22499999999999998 10-14 0.29 15-19 0.37 20-24 0.27 25-29 0.27999999999999997 30-34 0.22 35-39 0.26 40-44 0.27999999999999997 45-49 0.255 50-54 0.27 55-59 0.26 60-64 0.24 65-69 0.255 70-74 0.26 75-79 0.27499999999999997 80-84 0.24 85-89 0.22999999999999998 90-94 0.24 95-99 0.21 100-104 0.40499999999999997 105-109 0.59 110-114 0.5700000000000001 115-119 0.475 120-124 0.675 125-129 0.38 130-134 0.525 135-139 0.635 140-144 0.72 145-149 0.58 150-151 0.5375 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 64.625 #Duplication Level Percentage of deduplicated Percentage of total 1 82.66924564796905 53.425 2 8.085106382978724 10.45 3 2.630560928433269 5.1 4 2.282398452611219 5.8999999999999995 5 1.2379110251450676 4.0 6 0.6189555125725338 2.4 7 0.3481624758220503 1.575 8 0.5029013539651838 2.6 9 0.23210831721470018 1.35 >10 1.3926499032882012 13.200000000000001 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GCACAACATCCTTATGCATCCATTTCACATGTTAGGTGTAGCTGGTGTTT 26 0.65 No Hit GCTAGTTTCAACAACTCTCGTTCTTTACACTTCTTCTTGGCTGCTTGGCC 25 0.625 No Hit ATTATCTATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAAT 24 0.6 No Hit GTTATTTTGGCCGATTAATCTTCCAATATGCTAGTTTCAACAACTCTCGT 23 0.575 No Hit GTGGGAACTTAGTTTCCGTTTGGGTATGCGCCCTTGGATTGCTGTTGCAT 22 0.5499999999999999 No Hit ATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAA 21 0.525 No Hit GCTTGTTATATGGGTCGTGAGTGGGAACTTAGTTTCCGTTTGGGTATGCG 21 0.525 No Hit GGTACTTTCAACTTTATGATTGTATTCCAGGCAGAGCACAACATCCTTAT 21 0.525 No Hit GTTCAACCTAAATGGATTCAATTTCAACCAATCTGTAGTTGATAGTCAAG 18 0.44999999999999996 No Hit GCATGGTTCCTTGGTAACCTCTAGTTTGATCAGGGAAACTACTGAAAATG 17 0.42500000000000004 No Hit GGAAGCTTCTCTGATGGTATGCCTTTAGGAATATCTGGTACTTTCAACTT 17 0.42500000000000004 No Hit AGGGAAACTACTGAAAATGAATCTGCTAATGAGGGTTACAAATTTGGTCA 16 0.4 No Hit GTCGTGAGTGGGAACTTAGTTTCCGTTTGGGTATGCGCCCTTGGATTGCT 14 0.35000000000000003 No Hit GTTTCAACAACTCTCGTTCTTTACACTTCTTCTTGGCTGCTTGGCCTGTA 14 0.35000000000000003 No Hit ATTTGGTCAAGAGGAAGAGACTTATAATATTGTGGCTGCTCATGGTTATT 13 0.325 No Hit CTATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAA 13 0.325 No Hit GTTATACAATGGTGGTCCTTATGAGCTAATTGTTCTACACTTCTTACTTG 13 0.325 No Hit GTAGGAATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCT 13 0.325 No Hit GTTTCTGGTTCTTTACTTTATGGAAACAATATTATCTCTGGTGCTATTAT 13 0.325 No Hit GTTTGATCAGGGAAACTACTGAAAATGAATCTGCTAATGAGGGTTACAAA 13 0.325 No Hit GCTGCATCCGTTGATGAATGGTTATACAATGGTGGTCCTTATGAGCTAAT 12 0.3 No Hit GTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGATCTTACCATGAC 12 0.3 No Hit GTTTTGATGATCCCTACCTTATTGACCGCAACTTCTGTATTTATTATCGC 11 0.27499999999999997 No Hit GTAGCTTGTTATATGGGTCGTGAGTGGGAACTTAGTTTCCGTTTGGGTAT 11 0.27499999999999997 No Hit ATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCA 11 0.27499999999999997 No Hit GTTGCATATTCAGCTCCTGTTGCAGCTGCGACTGCTGTTTTCTTGATTTA 11 0.27499999999999997 No Hit GTAACCTCTAGTTTGATCAGGGAAACTACTGAAAATGAATCTGCTAATGA 11 0.27499999999999997 No Hit ATTCAGTGCTATGCATGGTTCCTTGGTAACCTCTAGTTTGATCAGGGAAA 11 0.27499999999999997 No Hit GGGAACTTAGTTTCCGTTTGGGTATGCGCCCTTGGATTGCTGTTGCATAT 11 0.27499999999999997 No Hit GCTAATTGTTCTACACTTCTTACTTGGTGTAGCTTGTTATATGGGTCGTG 10 0.25 No Hit GTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGATCTT 10 0.25 No Hit GACTTATAATATTGTGGCTGCTCATGGTTATTTTGGCCGATTAATCTTCC 10 0.25 No Hit GTTCGATCCTGGCTCAGGATGAACGCTGGCGGCATGCTTAACACATGCAA 10 0.25 No Hit TATCTATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTT 10 0.25 No Hit CTTCCAATATGCTAGTTTCAACAACTCTCGTTCTTTACACTTCTTCTTGG 10 0.25 No Hit ATCTATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTG 10 0.25 No Hit CCTAAATGGATTCAATTTCAACCAATCTGTAGTTGATAGTCAAGGTCGCG 9 0.22499999999999998 No Hit TATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAA 9 0.22499999999999998 No Hit GGGAAACTACTGAAAATGAATCTGCTAATGAGGGTTACAAATTTGGTCAA 9 0.22499999999999998 No Hit GTTACAAATTTGGTCAAGAGGAAGAGACTTATAATATTGTGGCTGCTCAT 9 0.22499999999999998 No Hit GTTGATGAATGGTTATACAATGGTGGTCCTTATGAGCTAATTGTTCTACA 9 0.22499999999999998 No Hit GAGCACAACATCCTTATGCATCCATTTCACATGTTAGGTGTAGCTGGTGT 9 0.22499999999999998 No Hit AGCACAACATCCTTATGCATCCATTTCACATGTTAGGTGTAGCTGGTGTT 8 0.2 No Hit GGAAGAGACTTATAATATTGTGGCTGCTCATGGTTATTTTGGCCGATTAA 8 0.2 No Hit ATCGGATTGCACTTTTACCCAATTTGGGAAGCTGCATCCGTTGATGAATG 8 0.2 No Hit GAGACTTATAATATTGTGGCTGCTCATGGTTATTTTGGCCGATTAATCTT 8 0.2 No Hit GGAATATCTGGTACTTTCAACTTTATGATTGTATTCCAGGCAGAGCACAA 8 0.2 No Hit TGAAAATGAATCTGCTAATGAGGGTTACAAATTTGGTCAAGAGGAAGAGA 8 0.2 No Hit AGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGATCT 8 0.2 No Hit GTGCTATGCATGGTTCCTTGGTAACCTCTAGTTTGATCAGGGAAACTACT 8 0.2 No Hit CAGTGCTATGCATGGTTCCTTGGTAACCTCTAGTTTGATCAGGGAAACTA 8 0.2 No Hit ATTCAGCTCCTGTTGCAGCTGCGACTGCTGTTTTCTTGATTTACCCTATT 8 0.2 No Hit CAGGGAAACTACTGAAAATGAATCTGCTAATGAGGGTTACAAATTTGGTC 8 0.2 No Hit GTTAGGTGTAGCTGGTGTTTTCGGCGGTTCCCTATTCAGTGCTATGCATG 8 0.2 No Hit AACATCCTTATGCATCCATTTCACATGTTAGGTGTAGCTGGTGTTTTCGG 8 0.2 No Hit GGTCGCTTCTGCAACTGGATAACTAGCACTGAAAATCGTCTTTACATCGG 7 0.17500000000000002 No Hit CATGACTGCAATTTTAGAGAGACGCGAAAGTACAAGCCTGTGGGGTCGCT 7 0.17500000000000002 No Hit CCTATTCAGTGCTATGCATGGTTCCTTGGTAACCTCTAGTTTGATCAGGG 7 0.17500000000000002 No Hit ATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCTAAATGG 7 0.17500000000000002 No Hit GGAATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCTAAA 7 0.17500000000000002 No Hit GTGTTTTCGGCGGTTCCCTATTCAGTGCTATGCATGGTTCCTTGGTAACC 7 0.17500000000000002 No Hit CTGAAAATGAATCTGCTAATGAGGGTTACAAATTTGGTCAAGAGGAAGAG 7 0.17500000000000002 No Hit GTTCTTTACTTTATGGAAACAATATTATCTCTGGTGCTATTATTCCTACT 7 0.17500000000000002 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 7 0.17500000000000002 No Hit ATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGATCTTACCATG 6 0.15 No Hit GCTCATGGTTATTTTGGCCGATTAATCTTCCAATATGCTAGTTTCAACAA 6 0.15 No Hit CTGGTACTTTCAACTTTATGATTGTATTCCAGGCAGAGCACAACATCCTT 6 0.15 No Hit GGAAACTACTGAAAATGAATCTGCTAATGAGGGTTACAAATTTGGTCAAG 6 0.15 No Hit GCTTGGGAGTCCTTGCAATTTGAATAAACCAAGATCTTACCATGACTGCA 6 0.15 No Hit CTGCGACTGCTGTTTTCTTGATTTACCCTATTGGTCAAGGAAGCTTCTCT 6 0.15 No Hit GATTAATCTTCCAATATGCTAGTTTCAACAACTCTCGTTCTTTACACTTC 6 0.15 No Hit GAGGGTTACAAATTTGGTCAAGAGGAAGAGACTTATAATATTGTGGCTGC 6 0.15 No Hit GGCCTGTAGTAGGAATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCG 6 0.15 No Hit CAATATGCTAGTTTCAACAACTCTCGTTCTTTACACTTCTTCTTGGCTGC 6 0.15 No Hit GCTGTTTTCTTGATTTACCCTATTGGTCAAGGAAGCTTCTCTGATGGTAT 6 0.15 No Hit AAACAATATTATCTCTGGTGCTATTATTCCTACTTCTGCGGCAATCGGAT 6 0.15 No Hit CACATGTTAGGTGTAGCTGGTGTTTTCGGCGGTTCCCTATTCAGTGCTAT 6 0.15 No Hit GTCAAGAGGAAGAGACTTATAATATTGTGGCTGCTCATGGTTATTTTGGC 6 0.15 No Hit TCTATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGA 6 0.15 No Hit GAATGGTTATACAATGGTGGTCCTTATGAGCTAATTGTTCTACACTTCTT 6 0.15 No Hit CTTGGGAGTCCTTGCAATTTGAATAAACCAAGATCTTACCATGACTGCAA 5 0.125 No Hit GTTTGGGTATGCGCCCTTGGATTGCTGTTGCATATTCAGCTCCTGTTGCA 5 0.125 No Hit GAAAATCGTCTTTACATCGGATGGTTCGGTGTTTTGATGATCCCTACCTT 5 0.125 No Hit GTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCTAAATGGATTCA 5 0.125 No Hit GGTAACCTCTAGTTTGATCAGGGAAACTACTGAAAATGAATCTGCTAATG 5 0.125 No Hit ATCTGCTAATGAGGGTTACAAATTTGGTCAAGAGGAAGAGACTTATAATA 5 0.125 No Hit CTCTCGTTCTTTACACTTCTTCTTGGCTGCTTGGCCTGTAGTAGGAATCT 5 0.125 No Hit AGTACTATGGCGTTCAACCTAAATGGATTCAATTTCAACCAATCTGTAGT 5 0.125 No Hit CCAAGATCTTACCATGACTGCAATTTTAGAGAGACGCGAAAGTACAAGCC 5 0.125 No Hit GTACTATGGCGTTCAACCTAAATGGATTCAATTTCAACCAATCTGTAGTT 5 0.125 No Hit ATCGCCTTCATCGCAGCCCCTCCAGTAGATATTGATGGTATTCGCGAGCC 5 0.125 No Hit CTTATGAGCTAATTGTTCTACACTTCTTACTTGGTGTAGCTTGTTATATG 5 0.125 No Hit GGCTGCTTGGCCTGTAGTAGGAATCTGGTTCACTGCTTTAGGTATTAGTA 5 0.125 No Hit CTTCTTCTTGGCTGCTTGGCCTGTAGTAGGAATCTGGTTCACTGCTTTAG 5 0.125 No Hit CTCTAGTTTGATCAGGGAAACTACTGAAAATGAATCTGCTAATGAGGGTT 5 0.125 No Hit GCTAATGAGGGTTACAAATTTGGTCAAGAGGAAGAGACTTATAATATTGT 5 0.125 No Hit ATTTATTATCGCCTTCATCGCAGCCCCTCCAGTAGATATTGATGGTATTC 5 0.125 No Hit CTTGTTATATGGGTCGTGAGTGGGAACTTAGTTTCCGTTTGGGTATGCGC 5 0.125 No Hit GTTGATAGTCAAGGTCGCGTTATTAATACTTGGGCTGATATCATCAACCG 5 0.125 No Hit GGAAACAATATTATCTCTGGTGCTATTATTCCTACTTCTGCGGCAATCGG 5 0.125 No Hit TATGCCTTTAGGAATATCTGGTACTTTCAACTTTATGATTGTATTCCAGG 5 0.125 No Hit GGATTGCACTTTTACCCAATTTGGGAAGCTGCATCCGTTGATGAATGGTT 5 0.125 No Hit GTTATTGTGAGAATTCTTAATTCAAGAGTTGTAAGGAGGGACTTATGTCA 5 0.125 No Hit GTTTCCGTTTGGGTATGCGCCCTTGGATTGCTGTTGCATATTCAGCTCCT 5 0.125 No Hit ATACAATGGTGGTCCTTATGAGCTAATTGTTCTACACTTCTTACTTGGTG 5 0.125 No Hit GTCAAGGTCGCGTTATTAATACTTGGGCTGATATCATCAACCGTGCTAAT 5 0.125 No Hit GGATAACTAGCACTGAAAATCGTCTTTACATCGGATGGTTCGGTGTTTTG 5 0.125 No Hit GAAGAGACTTATAATATTGTGGCTGCTCATGGTTATTTTGGCCGATTAAT 5 0.125 No Hit CTTCATCGCAGCCCCTCCAGTAGATATTGATGGTATTCGCGAGCCTGTTT 5 0.125 No Hit GAAGCTTCTCTGATGGTATGCCTTTAGGAATATCTGGTACTTTCAACTTT 5 0.125 No Hit CTTTATGATTGTATTCCAGGCAGAGCACAACATCCTTATGCATCCATTTC 5 0.125 No Hit GTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGATCTTACCATGACTG 5 0.125 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0125 0.0 0.0 0.0 0.0 50-51 0.025 0.0 0.0 0.0 0.0 52-53 0.025 0.0 0.0 0.0 0.0 54-55 0.025 0.0 0.0 0.0 0.0 56-57 0.025 0.0 0.0 0.0 0.0 58-59 0.025 0.0 0.0 0.0 0.0 60-61 0.025 0.0 0.0 0.0 0.0 62-63 0.025 0.0 0.0 0.0 0.0 64-65 0.025 0.0 0.0 0.0 0.0 66-67 0.025 0.0 0.0 0.0 0.0 68-69 0.025 0.0 0.0 0.0 0.0 70-71 0.025 0.0 0.0 0.0 0.0 72-73 0.05 0.0 0.0 0.0 0.0 74-75 0.05 0.0 0.0 0.0 0.0 76-77 0.05 0.0 0.0 0.0 0.0 78-79 0.0875 0.0 0.0 0.0 0.0 80-81 0.1125 0.0 0.0 0.0 0.0 82-83 0.15 0.0 0.0 0.0 0.0 84-85 0.16249999999999998 0.0 0.0 0.0 0.0 86-87 0.23750000000000002 0.0 0.0 0.0 0.0 88-89 0.38749999999999996 0.0 0.0 0.0 0.0 90-91 0.44999999999999996 0.0 0.0 0.0 0.0 92-93 0.5625 0.0 0.0 0.0 0.0 94-95 0.6875 0.0 0.0 0.0 0.0 96-97 0.7875000000000001 0.0 0.0 0.0 0.0 98-99 0.8375 0.0 0.0 0.0 0.0 100-101 0.925 0.0 0.0 0.0 0.0 102-103 1.0875 0.0 0.0 0.0 0.0 104-105 1.3125 0.0 0.0 0.0 0.0 106-107 1.5875 0.0 0.0 0.0 0.0 108-109 1.8 0.0 0.0 0.0 0.0 110-111 1.9874999999999998 0.0 0.0 0.0 0.0 112-113 2.125 0.0 0.0 0.0 0.0 114-115 2.4125 0.0 0.0 0.0 0.0 116-117 2.625 0.0 0.0 0.0 0.0 118-119 2.7375 0.0 0.0 0.0 0.0 120-121 2.95 0.0 0.0 0.0 0.0 122-123 3.3375000000000004 0.0 0.0 0.0 0.0 124-125 3.5625 0.0 0.0 0.0 0.0 126-127 3.8625 0.0 0.0 0.0 0.0 128-129 4.225 0.0 0.0 0.0 0.0 130-131 4.512499999999999 0.0 0.0 0.0 0.0 132-133 4.9125 0.0 0.0 0.0 0.0 134-135 5.2125 0.0 0.0 0.0 0.0 136-137 5.6625 0.0 0.0 0.0 0.0 138-139 6.0625 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AACTGGG 10 0.006830828 145.0 4 >>END_MODULE Read 1220417 spots for SRR6941553.sra Written 1220417 spots for SRR6941553.sra Read 1220417 spots for SRR6941553.sra Written 1220417 spots for SRR6941553.sra Read 1220417 spots for SRR6941553.sra Written 1220417 spots for SRR6941553.sra Read 1220417 spots for SRR6941553.sra Written 1220417 spots for SRR6941553.sra Read 1220417 spots for SRR6941553.sra Written 1220417 spots for SRR6941553.sra Read 1220417 spots for SRR6941553.sra Written 1220417 spots for SRR6941553.sra Read 1220417 spots for SRR6941553.sra Written 1220417 spots for SRR6941553.sra Read 1220417 spots for SRR6941553.sra Written 1220417 spots for SRR6941553.sra Read 1220417 spots for SRR6941553.sra Written 1220417 spots for SRR6941553.sra Read 1220417 spots for SRR6941553.sra Written 1220417 spots for SRR6941553.sra Read 1220417 spots for SRR6941553.sra Written 1220417 spots for SRR6941553.sra Read 1220417 spots for SRR6941553.sra Written 1220417 spots for SRR6941553.sra Read 1220417 spots for SRR6941553.sra Written 1220417 spots for SRR6941553.sra Read 1220417 spots for SRR6941553.sra Written 1220417 spots for SRR6941553.sra Read 1220417 spots for SRR6941553.sra Written 1220417 spots for SRR6941553.sra Read 1220417 spots for SRR6941553.sra Written 1220417 spots for SRR6941553.sra Read 1220417 spots for SRR6941553.sra Written 1220417 spots for SRR6941553.sra Read 1220435 spots for SRR6941553.sra Written 1220435 spots for SRR6941553.sra Read 1220417 spots for SRR6941553.sra Written 1220417 spots for SRR6941553.sra Read 1220417 spots for SRR6941553.sra Written 1220417 spots for SRR6941553.sra SRR ids: ['SRR6941553.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_vfqgt3um SRR6941553.sra spots: 24408358 blocks: [[1, 1220417], [1220418, 2440834], [2440835, 3661251], [3661252, 4881668], [4881669, 6102085], [6102086, 7322502], [7322503, 8542919], [8542920, 9763336], [9763337, 10983753], [10983754, 12204170], [12204171, 13424587], [13424588, 14645004], [14645005, 15865421], [15865422, 17085838], [17085839, 18306255], [18306256, 19526672], [19526673, 20747089], [20747090, 21967506], [21967507, 23187923], [23187924, 24408358]] SRR6941553 file size 8249491 SRR6941553 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR6941553 SRR6941553_1.fastq SRR6941553_2.fastq Input file: SRR6941553_1.fastq Paired file: SRR6941553_2.fastq trimmed: SRR6941553-trimmed-pair1.fastq, SRR6941553-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Thu Dec 12 02:23:07 2024 >> started Thu Dec 12 02:25:57 2024 >> done (169.748s) 24408358 read pairs processed; of these: 11407 ( 0.05%) short read pairs filtered out after trimming by size control 27544 ( 0.11%) empty read pairs filtered out after trimming by size control 24369407 (99.84%) read pairs available; of these: 3836339 (15.74%) trimmed read pairs available after processing 20533068 (84.26%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 19 5 0.00% 20 0 0.00% 21 2 0.00% 22 3 0.00% 23 8 0.00% 24 5 0.00% 25 7 0.00% 26 8 0.00% 27 7 0.00% 28 7 0.00% 29 14 0.00% 30 13 0.00% 31 9 0.00% 32 12 0.00% 33 15 0.00% 34 12 0.00% 35 18 0.00% 36 18 0.00% 37 26 0.00% 38 37 0.00% 39 37 0.00% 40 44 0.00% 41 63 0.00% 42 72 0.00% 43 52 0.00% 44 77 0.00% 45 68 0.00% 46 106 0.00% 47 133 0.00% 48 132 0.00% 49 167 0.00% 50 180 0.00% 51 189 0.00% 52 235 0.00% 53 263 0.00% 54 303 0.00% 55 326 0.00% 56 363 0.00% 57 412 0.00% 58 485 0.00% 59 525 0.00% 60 636 0.00% 61 761 0.00% 62 977 0.00% 63 1063 0.00% 64 1250 0.01% 65 1320 0.01% 66 1337 0.01% 67 1374 0.01% 68 1662 0.01% 69 2011 0.01% 70 2332 0.01% 71 2744 0.01% 72 3399 0.01% 73 3480 0.01% 74 3368 0.01% 75 4003 0.02% 76 3941 0.02% 77 4610 0.02% 78 4508 0.02% 79 5192 0.02% 80 6038 0.02% 81 6784 0.03% 82 7835 0.03% 83 8094 0.03% 84 8669 0.04% 85 10559 0.04% 86 10629 0.04% 87 11236 0.05% 88 12909 0.05% 89 12524 0.05% 90 14517 0.06% 91 15138 0.06% 92 17749 0.07% 93 17882 0.07% 94 19071 0.08% 95 20484 0.08% 96 19296 0.08% 97 19254 0.08% 98 19006 0.08% 99 20017 0.08% 100 21388 0.09% 101 23065 0.09% 102 25892 0.11% 103 25237 0.10% 104 27541 0.11% 105 28342 0.12% 106 28206 0.12% 107 28401 0.12% 108 29059 0.12% 109 30518 0.13% 110 31014 0.13% 111 34945 0.14% 112 35769 0.15% 113 34900 0.14% 114 38205 0.16% 115 35813 0.15% 116 37064 0.15% 117 34951 0.14% 118 35693 0.15% 119 34819 0.14% 120 36632 0.15% 121 37711 0.15% 122 45213 0.19% 123 45981 0.19% 124 46470 0.19% 125 46717 0.19% 126 44583 0.18% 127 43976 0.18% 128 43575 0.18% 129 48559 0.20% 130 46864 0.19% 131 50502 0.21% 132 51159 0.21% 133 46688 0.19% 134 54453 0.22% 135 51297 0.21% 136 54392 0.22% 137 52595 0.22% 138 59523 0.24% 139 59064 0.24% 140 57583 0.24% 141 70050 0.29% 142 60230 0.25% 143 66708 0.27% 144 65596 0.27% 145 77709 0.32% 146 84723 0.35% 147 90375 0.37% 148 116413 0.48% 149 169105 0.69% 150 1058954 4.35% 151 20533068 84.26% 24369407 reads passed initial QC criterion=sequence-density sequence-density=0.33 sequence-density-rank=1 fanout-score=5.43 fanout-score-rank=10 prefix-density=1.05 prefix-fanout=1.7 sequence=CAGCCTCACGCGGTGCCTGCCGCTCTAGGATCCGTGAGGCCCAGCTTTCGCATAGGCCCCAGCAGATCCACTACGCAA criterion=fanout-score sequence-density=0.02 sequence-density-rank=34 fanout-score=30.65 fanout-score-rank=1 prefix-density=0.34 prefix-fanout=2.1 sequence=AAAAACAGTAGAAGTAGAACAGGTATAAATAAGAAAATCTTAGTTAAGAGGGTTCATGTAAAGAACAGGTTCTAAATCACGATCGATTCCCTTTTCAAAACCTGCTGCAGCAGCTCGGGCTCTTCCTGCATGCCACAAATGGCCCACAAAAAAGAAGAATCCTAGAACAAAATGAGAAGTCGATAACCAACTTCTAGGAGAGACATAATTAACTGCATTGATCTCGGTAGCTACGCCACCCACGGAATTTAAAGAGCCTAAAGGAGCATGGGTCATATATTCCGCTGAACGTCGTTCTTGCCAAGGTTGTATGTCTTTTTTCAACCTACTCAAGTCCAAACCGTTGGGCCCCCTTAGAGGTTCTAACCATGGAGCACGGAGGTCCCAAAAACGCATAGTTTCCCCTCCAAAGATAACCTCTCCCGTTGGGGAACGCATTAGATATTTACCTAAACCTGTGGGTCCTTGAGCAGATCCCACATTAGCTCCAAGACGCTGGTCTCTAACTAGA criterion=sequence-density sequence-density=0.43 sequence-density-rank=1 fanout-score=1.97 fanout-score-rank=24 prefix-density=0.43 prefix-fanout=1.9 sequence=GCGTGAGGCTGG criterion=fanout-score sequence-density=0.01 sequence-density-rank=32 fanout-score=24.35 fanout-score-rank=1 prefix-density=0.05 prefix-fanout=6.4 sequence=TTTTTTTTTATGAGATTTTTGCTAAAGTTTCATTTACGCCTAATTCACATCGAGTAGACCTTGTTATTGTGAGAATTCTTAATTCAAGAGTTGTAAGGAGGGACTTATGTCACCACAAACAGAAACTAAAGCAAGTGTTGGATTTAAAGCTGGTGTTAAAGATTATAGATTGACTTACTACACCCCGGAGTATGAAACCAAGGATACTGATATCTTGGCAGCATTCCGAGTATCTCCTCAACCTGGGGTTCCGCCCGAAGAAGCAGGGGCTGCAGTAGCTGCCGAATCTTCTACTGGTACATGGACAACTGTTTGGACTGATGGACTTACTAGTCTTGATCGTTACAAAGGACGATGCTATCACATCGAGCCTGTTCCTGGGGAAGACAGTCAATGGATCTGTTATGTAGCTTATCCATTAGATCTATTTGAAGAGGGTTCCGTTACTAACATGTTTACTTCCATTGTAGGTAACGTATTTGGTTTCAAAGCCCTACGTGCTCTACGTCTG SRR6941553 testing PE reads STAR mapping to Ensembl genome Started job on | Dec 12 02:30:05 Started mapping on | Dec 12 02:30:06 Finished on | Dec 12 02:48:37 Mapping speed, Million of reads per hour | 78.96 Number of input reads | 24369407 Average input read length | 296 UNIQUE READS: Uniquely mapped reads number | 15050528 Uniquely mapped reads % | 61.76% Average mapped length | 296.37 Number of splices: Total | 2973610 Number of splices: Annotated (sjdb) | 2635738 Number of splices: GT/AG | 2769358 Number of splices: GC/AG | 35663 Number of splices: AT/AC | 13634 Number of splices: Non-canonical | 154955 Mismatch rate per base, % | 0.27% Deletion rate per base | 0.02% Deletion average length | 1.98 Insertion rate per base | 0.02% Insertion average length | 2.66 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 7571887 % of reads mapped to multiple loci | 31.07% Number of reads mapped to too many loci | 42803 % of reads mapped to too many loci | 0.18% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 6.11% % of reads unmapped: other | 0.89% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 1755400 1755400 1755400 N_multimapping 7571887 7571887 7571887 N_noFeature 6464026 14468791 6738659 N_ambiguous 577699 6365 280580 UnstrandedReadsAssigned:8008803 PositiveStrandReadsAssigned:575372 NegativeStrandReadsAssigned:8031289 Dataset is classified negative stranded MeadianReadLen=151 20thPercentileLength=151 echo kmer=147 SRR6941553 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,972 [index] number of k-mers: 66,720,672 [index] number of equivalence classes: 111,837 [quant] running in paired-end mode [quant] will process pair 1: SRR6941553-trimmed-pair1.fastq SRR6941553-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 24,369,407 reads, 12,984,098 reads pseudoaligned [quant] estimated average fragment length: 249.661 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,042 rounds 52973 SRR6941553.ke.tsv 35125 SRR6941553.se.tsv 88098 total ==> SRR6941553.ke.tsv <== target_id length eff_length est_counts tpm PNS24245 936 687.733 0 0 PNS24247 1044 795.339 15.3999 1.39201 PNS24249 1928 1679.34 2.78208 0.119099 PNS24246 1044 795.339 15.3999 1.39201 PNS24248 1044 795.339 15.3999 1.39201 PNS24244 1471 1222.34 50.0182 2.94181 PNS24243 293 96.9318 0 0 KQK14069 1603 1354.34 1710.92 90.8198 KQK14071 474 238.51 31.9811 9.63972 ==> SRR6941553.se.tsv <== BRADI_1g14170v3 2434 BRADI_1g53295v3 31 BRADI_1g59795v3 67 BRADI_1g07683v3 0 BRADI_1g00485v3 0 BRADI_1g20270v3 84 BRADI_1g74790v3 9 BRADI_1g09890v3 0 BRADI_1g77505v3 55 BRADI_1g48960v3 0 SRR6941553 completed mapping pipeline successfully