Starting /dee2/code/volunteer_pipeline.sh SRR6941554 current disk space = 1551495008256 free memory = 1537141648 SRR6941554 SRAfilesize f560f17a70b95ca404087baa2c5778f1 SRR6941554.sra SRR6941554.sra file validated SRR6941554 is paired end SRR6941554 is conventional basespace SRR6941554 read1 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR6941554_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 53 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 34.553 35.0 35.0 35.0 35.0 35.0 2 34.586 35.0 35.0 35.0 35.0 35.0 3 34.638 35.0 35.0 35.0 35.0 35.0 4 34.55575 35.0 35.0 35.0 33.0 35.0 5 34.55025 35.0 35.0 35.0 34.0 35.0 6 39.37875 40.0 40.0 40.0 39.0 40.0 7 39.3815 40.0 40.0 40.0 39.0 40.0 8 39.135 40.0 40.0 40.0 38.0 40.0 9 39.31375 40.0 40.0 40.0 39.0 40.0 10-14 39.3653 40.0 40.0 40.0 39.0 40.0 15-19 39.394850000000005 40.0 40.0 40.0 39.0 40.0 20-24 39.23555 40.0 40.0 40.0 38.8 40.0 25-29 39.30409999999999 40.0 40.0 40.0 39.0 40.0 30-34 39.33475 40.0 40.0 40.0 39.0 40.0 35-39 39.327600000000004 40.0 40.0 40.0 39.0 40.0 40-44 39.3375 40.0 40.0 40.0 39.0 40.0 45-49 39.240449999999996 40.0 40.0 40.0 38.8 40.0 50-54 39.286150000000006 40.0 40.0 40.0 39.0 40.0 55-59 39.29105 40.0 40.0 40.0 39.0 40.0 60-64 39.21169999999999 40.0 40.0 40.0 38.8 40.0 65-69 39.27205 40.0 40.0 40.0 39.0 40.0 70-74 39.22635 40.0 40.0 40.0 39.0 40.0 75-79 39.16825 40.0 40.0 40.0 38.6 40.0 80-84 39.1799 40.0 40.0 40.0 38.4 40.0 85-89 39.22825 40.0 40.0 40.0 39.0 40.0 90-94 39.20225000000001 40.0 40.0 40.0 39.0 40.0 95-99 39.04435 40.0 39.8 40.0 37.8 40.0 100-104 38.273399999999995 39.2 38.4 39.6 36.8 39.8 105-109 38.9698 40.0 39.4 40.0 37.6 40.0 110-114 39.09439999999999 40.0 40.0 40.0 38.0 40.0 115-119 39.15685 40.0 40.0 40.0 38.2 40.0 120-124 39.03815000000001 40.0 40.0 40.0 38.0 40.0 125-129 38.8485 40.0 39.4 40.0 37.2 40.0 130-134 38.6903 40.0 39.0 40.0 36.8 40.0 135-139 38.3914 40.0 39.0 40.0 36.2 40.0 140-144 38.1646 40.0 39.0 40.0 36.0 40.0 145-149 37.730000000000004 40.0 39.0 40.0 35.2 40.0 150-151 35.65975 39.0 36.5 39.5 30.0 40.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 21 1.0 22 0.0 23 1.0 24 1.0 25 2.0 26 4.0 27 6.0 28 16.0 29 18.0 30 21.0 31 21.0 32 45.0 33 48.0 34 60.0 35 81.0 36 123.0 37 172.0 38 315.0 39 3065.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 46.76529588766299 7.4473420260782355 2.908726178535607 42.878635907723165 2 20.3 7.625 32.6 39.475 3 17.075000000000003 11.375 26.6 44.95 4 24.05 18.575 19.825 37.55 5 25.1 25.5 23.175 26.224999999999998 6 24.55 25.3 25.324999999999996 24.825 7 17.599999999999998 20.325 41.199999999999996 20.875 8 21.375 17.0 33.525 28.1 9 20.849999999999998 18.05 33.6 27.500000000000004 10-14 22.53 21.865000000000002 27.425 28.18 15-19 24.21 20.395 25.465 29.93 20-24 24.094818963792758 22.089417883576715 25.470094018803763 28.345669133826767 25-29 24.195 19.97 26.415 29.42 30-34 24.154999999999998 19.580000000000002 25.145 31.119999999999997 35-39 22.400000000000002 20.330000000000002 27.155 30.115 40-44 23.383184114440052 21.3924873705797 24.45856049617366 30.765768018806583 45-49 24.385 18.905 25.365 31.345 50-54 24.625 19.66 25.990000000000002 29.725 55-59 22.96 21.685 26.974999999999998 28.38 60-64 25.945 19.54 26.35 28.165000000000003 65-69 23.845 22.98 24.185000000000002 28.99 70-74 23.505000000000003 22.259999999999998 24.86 29.375 75-79 24.605 23.98 23.400000000000002 28.015 80-84 23.185 24.715 23.82 28.28 85-89 24.85 23.549999999999997 22.725 28.875 90-94 26.384999999999998 23.355 22.055 28.205000000000002 95-99 25.355 23.119999999999997 23.225 28.299999999999997 100-104 24.715 22.71 24.104999999999997 28.470000000000002 105-109 23.18 23.015 24.55 29.255 110-114 22.68 24.425 24.654999999999998 28.24 115-119 22.31 24.085 24.740000000000002 28.865000000000002 120-124 22.365 26.16 22.189999999999998 29.285 125-129 22.43 24.545 23.02 30.005 130-134 23.5 22.075 23.055 31.369999999999997 135-139 21.62 23.46 25.06 29.86 140-144 22.295 23.95 24.38 29.375 145-149 21.255 25.369999999999997 24.725 28.65 150-151 20.599999999999998 26.25 24.6 28.549999999999997 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.5 23 0.5 24 0.0 25 0.0 26 0.5 27 1.0 28 0.5 29 0.0 30 1.0 31 1.0 32 2.0 33 2.5 34 2.0 35 4.5 36 16.5 37 24.0 38 26.0 39 37.0 40 40.5 41 31.5 42 32.5 43 47.0 44 52.5 45 66.5 46 87.0 47 88.0 48 75.0 49 92.5 50 134.0 51 175.0 52 183.0 53 214.5 54 292.0 55 424.0 56 466.0 57 322.5 58 261.5 59 264.0 60 176.0 61 90.5 62 59.0 63 50.0 64 37.0 65 20.0 66 14.5 67 8.5 68 9.5 69 8.5 70 11.5 71 14.0 72 11.5 73 7.0 74 3.0 75 2.0 76 4.0 77 3.0 78 0.5 79 0.0 80 0.0 81 0.5 82 0.5 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.02 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.034999999999999996 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.74999999999999 #Duplication Level Percentage of deduplicated Percentage of total 1 70.91787439613526 36.7 2 13.574879227053142 14.05 3 6.1352657004830915 9.525 4 2.3671497584541066 4.9 5 1.932367149758454 5.0 6 1.3526570048309179 4.2 7 0.6763285024154589 2.45 8 0.5797101449275363 2.4 9 0.43478260869565216 2.025 >10 1.932367149758454 15.174999999999999 >50 0.0966183574879227 3.5749999999999997 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GGCATGATGACTTGGCCTCATCCTCTCCTTCCTCCGGCTTAACACCGGCG 81 2.025 No Hit CCCCACTGCTGCCTCCCGTAGGAGTCTGGGCCGTGTCTCAGTCCCAGTGT 62 1.55 No Hit GGGGCATGATGACTTGGCCTCATCCTCTCCTTCCTCCGGCTTAACACCGG 35 0.8750000000000001 No Hit CCGGGAACGGATTCACCGCCGTATGGCTGACCGGCGATTACTAGCGATTC 28 0.7000000000000001 No Hit CCCACTGCTGCCTCCCGTAGGAGTCTGGGCCGTGTCTCAGTCCCAGTGTG 27 0.675 No Hit CCCGTCAATTCCTTTGAGTTTCATTCTTGCGAACGTACTCCCCAGGCGGG 26 0.65 No Hit CCTCAGCCTACGGGGTATTAGCAACCGTTTCCAGTTGTTGTTCCCCTCCC 25 0.625 No Hit GTCCAGTCAACTGCTGCGCCTCAACGCATTTCGGGGAGAACCAGCTAGCT 24 0.6 No Hit GTCCCTATTAATCATTACTCCGATCCCGAAGGCCAACACAATAGGACCGG 22 0.5499999999999999 No Hit GCCACCTACAGACGCTTTACGCCCAATCATTCCGGATAACGCTTGCATCC 20 0.5 No Hit GCCCAATCATTCCGGATAACGCTTGCATCCTCTGTCTTACCGCGGCTGCT 18 0.44999999999999996 No Hit GTTCGAGCTTTTCCTGGGAGTATGGCATCGGTTACATACTTCAGTGCCGT 17 0.42500000000000004 No Hit CCTCGCGGTACTTGTTCGCTATCGGTCTCTCGCCTGTATTTAGCCTTGGA 17 0.42500000000000004 No Hit GACCTGTTGTCCATCGACTACGCCTTTCGGCCTGATCTTAGGCCCTGACT 16 0.4 No Hit GTCAATTCCTTTGAGTTTCATTCTTGCGAACGTACTCCCCAGGCGGGATA 15 0.375 No Hit CTCCTTTTGCTCCTCAGCCTACGGGGTATTAGCAACCGTTTCCAGTTGTT 15 0.375 No Hit GTCCCAGTGTGGCTGATCATCCTCTCGGACCAGCTACTGATCATCGCCTT 15 0.375 No Hit CGTCAATTCCTTTGAGTTTCATTCTTGCGAACGTACTCCCCAGGCGGGAT 14 0.35000000000000003 No Hit GGGCATGATGACTTGGCCTCATCCTCTCCTTCCTCCGGCTTAACACCGGC 14 0.35000000000000003 No Hit GTTCTATTTCACTACCCACTGGGGGTTCTTTTCACCTTTCCCTCACGGTA 13 0.325 No Hit CCTTAAACCTATAACCATCTTTCGGCTAACCTAGCCTCCTCCGTCCCTCC 13 0.325 No Hit CCCTACCGTACTCCAGCTTGGTAGTTTCCACCGCCTGTCCAGGGTTGAGC 13 0.325 No Hit CTCCACGCGGCATTGCTCCGTCAGGCTTTCGCCCATTGCGGAAAATTCCC 13 0.325 No Hit GCATGATGACTTGGCCTCATCCTCTCCTTCCTCCGGCTTAACACCGGCGG 12 0.3 No Hit GGTCGTTCGAGCTTTTCCTGGGAGTATGGCATCGGTTACATACTTCAGTG 12 0.3 No Hit CCTAGATGTCCAGTCAACTGCTGCGCCTCAACGCATTTCGGGGAGAACCA 11 0.27499999999999997 No Hit CCGTCAATTCCTTTGAGTTTCATTCTTGCGAACGTACTCCCCAGGCGGGA 11 0.27499999999999997 No Hit GTCATTGTTTCTTCTCCGAGAAAAGAAGTTGACGACCCGTAGGCCTTCCA 11 0.27499999999999997 No Hit GTTCGTTCGTTAGGATGCCTCAGCTGCATACATCACTGCACTTCCACTTG 11 0.27499999999999997 No Hit GTACAAAGGGCAGGGACGTAGTCAACGCGAGCTGATGACTCGCGCTTACT 11 0.27499999999999997 No Hit GTCGGCATCGTTTATGGTTGAGACTAGGACGGTATCTGATCGTCTTCGAG 11 0.27499999999999997 No Hit GTGGCAACTAAACACGAGGGTTGCGCTCGTTGCGAGACTTAACCCAACAC 11 0.27499999999999997 No Hit CCCGGAACCCAAAGACTTTGATTTCTCATAAGGTGCCGGCGGAGTCCTAT 11 0.27499999999999997 No Hit GCCCCCGTCAATTCCTTTGAGTTTCATTCTTGCGAACGTACTCCCCAGGC 11 0.27499999999999997 No Hit GTTCAGGGTTCCAAACTCATAGTGGCAACTAAACACGAGGGTTGCGCTCG 11 0.27499999999999997 No Hit GCTCCTCAGCCTACGGGGTATTAGCAACCGTTTCCAGTTGTTGTTCCCCT 11 0.27499999999999997 No Hit CCCTAGAGTAACTTTTATCCGTTGAGCGACGGCCCTTCCACTCGGCACCG 11 0.27499999999999997 No Hit GCTCATTCTTCAACAGGCACGCGGTCAGAGATCACTTTCCCCTCCCACTG 11 0.27499999999999997 No Hit GTCGGTTTCGGGTACAGGTACCCTTTTGTTGAAGGTCGTTCGAGCTTTTC 10 0.25 No Hit GTTCGTTAACGGAATTAACCAGACAAATCGCTCCACCAACTAAGAACGGC 10 0.25 No Hit CCTAGCTTTCGTCTCTCAGTGTCAGTGTCGGCCCAGCAGAGTGCTTTCGC 10 0.25 No Hit CTTTCTTTTCCTCTGGCTACTAAGATGTTTCAGTTCGCCAGGTTGTCTCT 10 0.25 No Hit CTTGCAAGGTGGTCCTTGCTGATTCACACGGGATTCCACGTGCCCCATGC 9 0.22499999999999998 No Hit CCTGTATTTAGCCTTGGACGGAGTCTACCGCCCGATTTGGGCTGCATTCC 9 0.22499999999999998 No Hit GTGCTCTGCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTCCTA 9 0.22499999999999998 No Hit GCTCCCCTAGCTTTCGTCTCTCAGTGTCAGTGTCGGCCCAGCAGAGTGCT 9 0.22499999999999998 No Hit GTTCGCTATCGGTCTCTCGCCTGTATTTAGCCTTGGACGGAGTCTACCGC 9 0.22499999999999998 No Hit GCGGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTACG 9 0.22499999999999998 No Hit GCTTTCTTTTCCTCTGGCTACTAAGATGTTTCAGTTCGCCAGGTTGTCTC 9 0.22499999999999998 No Hit CTGTTGTCCATCGACTACGCCTTTCGGCCTGATCTTAGGCCCTGACTCAC 9 0.22499999999999998 No Hit GTCCTTAAACCTATAACCATCTTTCGGCTAACCTAGCCTCCTCCGTCCCT 9 0.22499999999999998 No Hit CCCAATCATTCCGGATAACGCTTGCATCCTCTGTCTTACCGCGGCTGCTG 8 0.2 No Hit GGCGGTGTGTACAAAGGGCAGGGACGTAGTCAACGCGAGCTGATGACTCG 8 0.2 No Hit GGCATAAGGGGCATGATGACTTGGCCTCATCCTCTCCTTCCTCCGGCTTA 8 0.2 No Hit CCCGACTGTCCCTATTAATCATTACTCCGATCCCGAAGGCCAACACAATA 8 0.2 No Hit GTTCCCTTAACCAAGCCACTGCCTATGAGTCGCCGGCTCATTCTTCAACA 8 0.2 No Hit GTCTCTCAGTGTCAGTGTCGGCCCAGCAGAGTGCTTTCGCCGTTGGTGTT 8 0.2 No Hit GCCATTCGCAGTTTCACAGTTCAAATTAGTTCATACTTGCACATGCATGG 8 0.2 No Hit CTATTTGGGAATCTCCGGATCTATGCTTATTTTCAACTCCCCGAAGCATT 8 0.2 No Hit CCGTTCGCCACTGGAAACACCACTTCCCGTTCGACTTGCATGTGTTAAGC 8 0.2 No Hit CACCTCCACGCGGCATTGCTCCGTCAGGCTTTCGCCCATTGCGGAAAATT 8 0.2 No Hit CCGCATTAATGGGCGAACAGCCCAACCCTTGGAACCACCTACAGCTCCAG 8 0.2 No Hit CTCAGCCTACGGGGTATTAGCAACCGTTTCCAGTTGTTGTTCCCCTCCCA 8 0.2 No Hit CCACTGCTGCCTCCCGTAGGAGTCTGGGCCGTGTCTCAGTCCCAGTGTGG 7 0.17500000000000002 No Hit ATCGACTACGCCTTTCGGCCTGATCTTAGGCCCTGACTCACCCTCCGTGG 7 0.17500000000000002 No Hit GTCCCTCTAAGAAGCTAGCTGCGGAGGGATGGCTCCGCATAGCTAGTTAG 7 0.17500000000000002 No Hit GTTCATCTTCAGCGCAAGGGCGCTCGATCAGTGAGCTATTACGCACTCTT 7 0.17500000000000002 No Hit CACGTGTGTCGCCCAGGGCATAAGGGGCATGATGACTTGGCCTCATCCTC 7 0.17500000000000002 No Hit CGGGAACGGATTCACCGCCGTATGGCTGACCGGCGATTACTAGCGATTCC 7 0.17500000000000002 No Hit GTCGAGTTATCATGAATCATCGGATCAGCGAGCAAAGCCCGCGTCAGCCT 7 0.17500000000000002 No Hit GTCCTCTCAATGCTCTAACGCCCACACCGGATATGGACCGAACTGTCTCA 7 0.17500000000000002 No Hit GTGTCCTTAAACCTATAACCATCTTTCGGCTAACCTAGCCTCCTCCGTCC 7 0.17500000000000002 No Hit GTACAAGGCCCGGGAACGGATTCACCGCCGTATGGCTGACCGGCGATTAC 7 0.17500000000000002 No Hit GTCGGTTCGGACCTCTGCTTAGTTTCATCCAAGCTTCATCCTGGTCATGG 7 0.17500000000000002 No Hit CCGGAACCCAAAGACTTTGATTTCTCATAAGGTGCCGGCGGAGTCCTATA 7 0.17500000000000002 No Hit CCTGTGTCGGTTTCGGGTACAGGTACCCTTTTGTTGAAGGTCGTTCGAGC 7 0.17500000000000002 No Hit CTCTGCCCCTACCGTACTCCAGCTTGGTAGTTTCCACCGCCTGTCCAGGG 7 0.17500000000000002 No Hit CACCGCTTGTGCGGGCCCCCGTCAATTCCTTTGAGTTTCATTCTTGCGAA 6 0.15 No Hit CCAGACTACAATTCGGACGGCACGGCCGCCCGATTCTCAAGCTGGGCTGC 6 0.15 No Hit CACCTACAGACGCTTTACGCCCAATCATTCCGGATAACGCTTGCATCCTC 6 0.15 No Hit GCCCCGTTCATCTTCAGCGCAAGGGCGCTCGATCAGTGAGCTATTACGCA 6 0.15 No Hit CCATCGACTACGCCTTTCGGCCTGATCTTAGGCCCTGACTCACCCTCCGT 6 0.15 No Hit CTCCTTTATCACTGAGCGGTCATTTAGGGGCCTTAGCTGGTGATCCGGGC 6 0.15 No Hit GTCAGTGTCGGCCCAGCAGAGTGCTTTCGCCGTTGGTGTTCTTTCCGATC 6 0.15 No Hit GTGTCGGTTTCGGGTACAGGTACCCTTTTGTTGAAGGTCGTTCGAGCTTT 6 0.15 No Hit GTCGGGGCAGGCGGCGGGCGCAGGCGCCGCTTGCTAGCTTGGATTCTGAC 6 0.15 No Hit GTTCTTTTCACCTTTCCCTCACGGTACTACTTCGCTATCGGTCACCCAGG 6 0.15 No Hit GACCTATTTGGGAATCTCCGGATCTATGCTTATTTTCAACTCCCCGAAGC 6 0.15 No Hit CCTCACGGTACTACTTCGCTATCGGTCACCCAGGAGTATTTAGCCTTGCA 6 0.15 No Hit CCTAGAGTAACTTTTATCCGTTGAGCGACGGCCCTTCCACTCGGCACCGT 6 0.15 No Hit CCATCGTTTACGGCTAGGACTACTGGGGTCTCTAATCCCATTTGCTCCCC 6 0.15 No Hit GTACCGCTCGCGCAGCCCGCACCGAAACAGTGCTTTACCCCTAGATGTCC 6 0.15 No Hit CCTAATTCTCCGTCACCCGTCACCACCATGGTAGGCCCCTATCCTACCAT 6 0.15 No Hit GGGCTGTTTCCCTCTCGACGATGAAGCTTATCCCCCATCGTCTCACTGGC 6 0.15 No Hit GTCGTTCGAGCTTTTCCTGGGAGTATGGCATCGGTTACATACTTCAGTGC 6 0.15 No Hit GCCCTATGAAGACTCGCTTTCGCTACGGCTCCGGTGGGTTCCGTTCCCTT 6 0.15 No Hit GTTCCGTTCCCTTAACCAAGCCACTGCCTATGAGTCGCCGGCTCATTCTT 6 0.15 No Hit AGCACGTGTGTCGCCCAGGGCATAAGGGGCATGATGACTTGGCCTCATCC 6 0.15 No Hit GGCGGTGTGTACAAGGCCCGGGAACGGATTCACCGCCGTATGGCTGACCG 6 0.15 No Hit CGTCATTGTTTCTTCTCCGAGAAAAGAAGTTGACGACCCGTAGGCCTTCC 6 0.15 No Hit GCCCATTGTAGCACGTGTGTCGCCCAGGGCATAAGGGGCATGATGACTTG 6 0.15 No Hit GTTTACGGCTAGGACTACTGGGGTCTCTAATCCCATTTGCTCCCCTAGCT 6 0.15 No Hit GTCTGTTCAGGGTTCCAAACTCATAGTGGCAACTAAACACGAGGGTTGCG 6 0.15 No Hit GCTCTGCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTCCTAAA 6 0.15 No Hit GTGGGCTTACTACTTATATGCTTTCAGCAGTTATCCTCTCCGCACTTGGC 6 0.15 No Hit CTCCAGACTACAATTCGGACGGCACGGCCGCCCGATTCTCAAGCTGGGCT 5 0.125 No Hit CCCGAAGTTACGGATCCGTTTTGCCGACTTCCCTTGCCTACATTGTTCCA 5 0.125 No Hit CTCCACCGCTTGTGCGGGCCCCCGTCAATTCCTTTGAGTTTCATTCTTGC 5 0.125 No Hit GCATCGTTTATGGTTGAGACTAGGACGGTATCTGATCGTCTTCGAGCCCC 5 0.125 No Hit CCAGTGTGGCTGATCATCCTCTCGGACCAGCTACTGATCATCGCCTTGGT 5 0.125 No Hit GTCCGGTCAACTGCTGCGCCTCAACGCATTTCGGGGAGAACCAGCTAGCT 5 0.125 No Hit CAACACCTTACGGCACGAGCTGACGACAGCCATGCACCACCTGTGTCCGC 5 0.125 No Hit GCTCCGTCAGGCTTTCGCCCATTGCGGAAAATTCCCCACTGCTGCCTCCC 5 0.125 No Hit CTCGCCGTTACTAGGGGAATCCTTGTAAGTTTCTTCTCCTCCGCTTATTT 5 0.125 No Hit GGGGTGGGCTTACTACTTATATGCTTTCAGCAGTTATCCTCTCCGCACTT 5 0.125 No Hit GATGACTTGGCCTCATCCTCTCCTTCCTCCGGCTTAACACCGGCGGTCTG 5 0.125 No Hit GCCCCATAGAAACTGTCTACCTGAGACTGTCCCTTGGCCCGCGGGTCTGA 5 0.125 No Hit GCTTTGAGCACTCTAATTTCTTCAAAGTAACGATGCCGGAGGCACGACCC 5 0.125 No Hit CTTTTGCTCCTCAGCCTACGGGGTATTAGCAACCGTTTCCAGTTGTTGTT 5 0.125 No Hit GCTTGTATTGCTCTCCCACAACCCCGTTTTCACGGTTTAGGCTGCTCCCA 5 0.125 No Hit CGGTCACCCAGGAGTATTTAGCCTTGCAAGGTGGTCCTTGCTGATTCACA 5 0.125 No Hit CGTTAACGGAATTAACCAGACAAATCGCTCCACCAACTAAGAACGGCCAT 5 0.125 No Hit CCCGTTTTCACGGTTTAGGCTGCTCCCATTTCGCTCGCCGCTACTACGGG 5 0.125 No Hit GTCCATCGACTACGCCTTTCGGCCTGATCTTAGGCCCTGACTCACCCTCC 5 0.125 No Hit ATCGTTTACGGCTAGGACTACTGGGGTCTCTAATCCCATTTGCTCCCCTA 5 0.125 No Hit CCTCAGATACCGTCATTGTTTCTTCTCCGAGAAAAGAAGTTGACGACCCG 5 0.125 No Hit GGAACGGATTCACCGCCGTATGGCTGACCGGCGATTACTAGCGATTCCTG 5 0.125 No Hit CCGGCTCATTCTTCAACAGGCACGCGGTCAGAGATCACTTTCCCCTCCCA 5 0.125 No Hit GTTTCTTCTCCGAGAAAAGAAGTTGACGACCCGTAGGCCTTCCACCTCCA 5 0.125 No Hit CCTGGGAGTATGGCATCGGTTACATACTTCAGTGCCGTAGCGCCTGGTAT 5 0.125 No Hit ATTTGGGAATCTCCGGATCTATGCTTATTTTCAACTCCCCGAAGCATTTC 5 0.125 No Hit CTAGCTTTCGTCTCTCAGTGTCAGTGTCGGCCCAGCAGAGTGCTTTCGCC 5 0.125 No Hit CCCCTACCGTACTCCAGCTTGGTAGTTTCCACCGCCTGTCCAGGGTTGAG 5 0.125 No Hit CACCCGTTCGCCACTGGAAACACCACTTCCCGTTCGACTTGCATGTGTTA 5 0.125 No Hit CTCGATTTGGTACCGCTCGCGCAGCCCGCACCGAAACAGTGCTTTACCCC 5 0.125 No Hit ACCTGTTGTCCATCGACTACGCCTTTCGGCCTGATCTTAGGCCCTGACTC 5 0.125 No Hit CCCTAGCTTTCGTCTCTCAGTGTCAGTGTCGGCCCAGCAGAGTGCTTTCG 5 0.125 No Hit GGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTACGTT 5 0.125 No Hit GGTGTGTACAAAGGGCAGGGACGTAGTCAACGCGAGCTGATGACTCGCGC 5 0.125 No Hit GCCACGCTTTCACGGTTCGTATTCGTACTGGAAATCAGAATCAAACGAGC 5 0.125 No Hit GGCAACTAAACACGAGGGTTGCGCTCGTTGCGAGACTTAACCCAACACCT 5 0.125 No Hit GCCTCACCAACTAGCTAATCAGACGCGAGCCCCTCCTTGGGCGGATTTCT 5 0.125 No Hit CTCTGCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTCCTAAAG 5 0.125 No Hit CCAACACCTTACGGCACGAGCTGACGACAGCCATGCACCACCTGTGTCCG 5 0.125 No Hit ATGATGACTTGGCCTCATCCTCTCCTTCCTCCGGCTTAACACCGGCGGTC 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.025 0.0 0.0 0.0 0.0 30-31 0.025 0.0 0.0 0.0 0.0 32-33 0.025 0.0 0.0 0.0 0.0 34-35 0.037500000000000006 0.0 0.0 0.0 0.0 36-37 0.05 0.0 0.0 0.0 0.0 38-39 0.075 0.0 0.0 0.0 0.0 40-41 0.075 0.0 0.0 0.0 0.0 42-43 0.075 0.0 0.0 0.0 0.0 44-45 0.125 0.0 0.0 0.0 0.0 46-47 0.15 0.0 0.0 0.0 0.0 48-49 0.21250000000000002 0.0 0.0 0.0 0.0 50-51 0.25 0.0 0.0 0.0 0.0 52-53 0.42500000000000004 0.0 0.0 0.0 0.0 54-55 0.525 0.0 0.0 0.0 0.0 56-57 0.6125 0.0 0.0 0.0 0.0 58-59 0.8 0.0 0.0 0.0 0.0 60-61 0.9375 0.0 0.0 0.0 0.0 62-63 1.15 0.0 0.0 0.0 0.0 64-65 1.3375 0.0 0.0 0.0 0.0 66-67 1.575 0.0 0.0 0.0 0.0 68-69 1.8624999999999998 0.0 0.0 0.0 0.0 70-71 2.2125000000000004 0.0 0.0 0.0 0.0 72-73 2.6625 0.0 0.0 0.0 0.0 74-75 3.4 0.0 0.0 0.0 0.0 76-77 4.05 0.0 0.0 0.0 0.0 78-79 4.824999999999999 0.0 0.0 0.0 0.0 80-81 5.775 0.0 0.0 0.0 0.0 82-83 6.725 0.0 0.0 0.0 0.0 84-85 7.5375 0.0 0.0 0.0 0.0 86-87 8.925 0.0 0.0 0.0 0.0 88-89 10.225 0.0 0.0 0.0 0.0 90-91 11.5125 0.0 0.0 0.0 0.0 92-93 12.6875 0.0 0.0 0.0 0.0 94-95 13.9375 0.0 0.0 0.0 0.0 96-97 15.274999999999999 0.0 0.0 0.0 0.0 98-99 16.762500000000003 0.0 0.0 0.0 0.0 100-101 18.125 0.0 0.0 0.0 0.0 102-103 19.45 0.0 0.0 0.0 0.0 104-105 21.0375 0.0 0.0 0.0 0.0 106-107 22.3375 0.0 0.0 0.0 0.0 108-109 23.7375 0.0 0.0 0.0 0.0 110-111 25.0375 0.0 0.0 0.0 0.0 112-113 26.6875 0.0 0.0 0.0 0.0 114-115 28.1375 0.0 0.0 0.0 0.0 116-117 29.8625 0.0 0.0 0.0 0.0 118-119 31.1875 0.0 0.0 0.0 0.0 120-121 32.3 0.0 0.0 0.0 0.0 122-123 33.4375 0.0 0.0 0.0 0.0 124-125 34.7625 0.0 0.0 0.0 0.0 126-127 35.925 0.0 0.0 0.0 0.0 128-129 37.150000000000006 0.0 0.0 0.0 0.0 130-131 38.25 0.0 0.0 0.0 0.0 132-133 39.25 0.0 0.0 0.0 0.0 134-135 40.2125 0.0 0.0 0.0 0.0 136-137 41.3125 0.0 0.0 0.0 0.0 138-139 42.2875 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CATGATG 20 1.8793453E-6 145.0 3 GGCATGA 20 1.8793453E-6 145.0 1 TGACTTG 20 1.8793453E-6 145.0 8 ATGACTT 20 1.8793453E-6 145.0 7 ATGATGA 20 1.8793453E-6 145.0 4 GCATGAT 20 1.8793453E-6 145.0 2 GACCATT 10 0.006830828 145.0 2 GACTTGG 25 5.7044963E-6 116.0 9 ACAGCCA 20 3.5877043E-4 108.75 145 TGATGAC 30 1.4118372E-5 96.666664 5 GATGACT 30 1.4118372E-5 96.666664 6 GGCCTCA 20 0.00593511 29.0 10-14 TTGGCCT 20 0.00593511 29.0 10-14 TGGCCTC 20 0.00593511 29.0 10-14 CTCGTAT 110 0.008585102 26.363638 145 AAAAAAA 145 1.2732926E-11 25.000002 145 AAACTCA 30 0.0014437955 24.166668 65-69 ACACCGG 30 0.0014437955 24.166668 40-44 TCATCCT 40 0.0076550315 18.125 3 >>END_MODULE SRR6941554 read2 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR6941554_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 53 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 34.27175 35.0 35.0 35.0 33.0 35.0 2 34.2615 35.0 35.0 35.0 33.0 35.0 3 34.18325 35.0 35.0 35.0 33.0 35.0 4 34.41425 35.0 35.0 35.0 34.0 35.0 5 34.44125 35.0 35.0 35.0 34.0 35.0 6 39.054 40.0 40.0 40.0 39.0 40.0 7 39.19275 40.0 40.0 40.0 39.0 40.0 8 39.21325 40.0 40.0 40.0 39.0 40.0 9 39.25225 40.0 40.0 40.0 39.0 40.0 10-14 39.18575 40.0 40.0 40.0 39.0 40.0 15-19 38.9518 40.0 40.0 40.0 38.4 40.0 20-24 39.237199999999994 40.0 40.0 40.0 38.8 40.0 25-29 39.17155 40.0 40.0 40.0 39.0 40.0 30-34 39.13555 40.0 40.0 40.0 39.0 40.0 35-39 39.13375 40.0 40.0 40.0 38.6 40.0 40-44 39.20865 40.0 40.0 40.0 39.0 40.0 45-49 38.972500000000004 40.0 39.8 40.0 38.4 40.0 50-54 39.113749999999996 40.0 40.0 40.0 38.8 40.0 55-59 39.0482 40.0 40.0 40.0 38.6 40.0 60-64 39.123450000000005 40.0 40.0 40.0 39.0 40.0 65-69 39.044650000000004 40.0 39.8 40.0 38.8 40.0 70-74 38.8304 40.0 39.4 40.0 37.4 40.0 75-79 39.0206 40.0 40.0 40.0 38.6 40.0 80-84 39.0094 40.0 40.0 40.0 38.2 40.0 85-89 38.67595 40.0 39.0 40.0 37.0 40.0 90-94 38.632450000000006 40.0 39.0 40.0 36.8 40.0 95-99 38.47725 40.0 39.0 40.0 35.8 40.0 100-104 37.223499999999994 38.6 37.6 39.2 33.6 39.6 105-109 38.130649999999996 40.0 39.0 40.0 35.4 40.0 110-114 37.970549999999996 40.0 39.0 40.0 35.2 40.0 115-119 37.57625 40.0 38.8 40.0 33.4 40.0 120-124 37.3796 40.0 39.0 40.0 32.8 40.0 125-129 36.6851 39.8 37.8 40.0 31.0 40.0 130-134 36.699400000000004 40.0 38.6 40.0 31.0 40.0 135-139 35.723850000000006 39.6 37.4 40.0 25.0 40.0 140-144 34.78060000000001 39.0 36.0 40.0 16.6 40.0 145-149 33.085 39.0 34.4 40.0 4.2 40.0 150-151 29.026625 36.5 18.0 39.5 2.0 40.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 8.0 3 3.0 4 0.0 5 0.0 6 0.0 7 0.0 8 1.0 9 1.0 10 0.0 11 1.0 12 0.0 13 0.0 14 1.0 15 2.0 16 1.0 17 0.0 18 0.0 19 2.0 20 4.0 21 2.0 22 3.0 23 10.0 24 11.0 25 9.0 26 18.0 27 31.0 28 28.0 29 26.0 30 51.0 31 53.0 32 58.0 33 72.0 34 98.0 35 120.0 36 140.0 37 290.0 38 529.0 39 2427.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 50.23935500125977 17.233560090702948 4.383975812547241 28.143109095490047 2 33.60100376411543 21.279799247176914 25.345043914680048 19.774153074027602 3 23.96694214876033 22.539444027047335 29.551715502128722 23.94189832206361 4 29.7208951470958 29.117425194870506 19.763640935378426 21.39803872265527 5 32.40531728116378 30.825181840983195 16.15249561073489 20.617005267118135 6 27.366306803916647 35.24981169972383 15.89254330906352 21.49133818729601 7 24.391468005018822 20.978670012547052 30.138017565872023 24.49184441656211 8 26.960661488348787 23.277374091706342 21.398145828113254 28.36381859183162 9 28.782565130260522 20.96693386773547 24.02304609218437 26.22745490981964 10-14 30.216115930401642 24.504838790553077 20.648849220277793 24.63019605876749 15-19 30.1048828223014 25.257188738896975 21.60887238420234 23.029056054599288 20-24 29.082067478818868 25.372236426530304 21.451847395598335 24.09384869905249 25-29 30.739533717723738 24.88844321885184 20.857357733767863 23.514665329656555 30-34 29.573583203888358 26.0459988976299 20.584256150724055 23.79616174775768 35-39 29.554229554229554 26.34508348794063 21.024921024921024 23.07576593290879 40-44 29.771872649786914 26.25720732013036 20.39608924542492 23.57483078465781 45-49 29.636500376034093 26.03660065179243 20.17046878917022 24.15643018300326 50-54 30.387045021558208 24.952371402787527 21.5832748420736 23.07730873358067 55-59 29.2719614921781 26.00280786201364 21.274568792619334 23.45066185318893 60-64 30.471177944862156 25.238095238095237 21.438596491228072 22.85213032581454 65-69 30.494285141367556 25.37597754160818 20.95949468618408 23.170242630840185 70-74 30.78967159689145 25.76084231637002 20.787164702933065 22.662321383805466 75-79 30.618794504061782 24.49603851168388 20.995888075418716 23.889278908835625 80-84 30.349355922008918 25.667886321487643 21.47762016941507 22.505137587088367 85-89 30.754196943122025 25.968428965171636 20.42094713104485 22.856426960661487 90-94 31.920012028266427 26.29679747406405 19.976945822683305 21.806244674986218 95-99 32.9760056103792 25.33687321544858 20.44782848269298 21.239292691479235 100-104 32.41303147432358 27.649214396867627 19.712865819988956 20.22488830881984 105-109 32.89910471783523 25.701639674077054 20.682023941253394 20.717231666834323 110-114 32.95648678524771 26.394332227916795 20.49040297457542 20.158778012260075 115-119 33.666449053382216 26.74634660774369 19.881484457389647 19.705719881484455 120-124 34.512428298279154 26.512025762302503 19.81483345073966 19.160712488678673 125-129 33.6126863050133 27.219350629798768 19.83238821699202 19.335574848195915 130-134 34.53887884267631 26.220614828209765 20.44404259594133 18.796463733172594 135-139 34.07593663565502 26.411868242393766 20.40231330148353 19.109881820467688 140-144 34.619258662369056 26.390008058017727 21.28323932312651 17.707493956486704 145-149 33.62155640458476 27.22199879348482 21.450834506334203 17.70561029559622 150-151 34.86999120713478 27.798015324707954 18.515261901771137 18.81673156638613 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 6.0 1 3.5 2 1.0 3 0.5 4 0.0 5 0.5 6 0.5 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.5 16 0.5 17 0.0 18 0.0 19 0.0 20 0.0 21 0.5 22 0.5 23 0.5 24 1.0 25 0.5 26 0.0 27 0.5 28 0.5 29 0.0 30 1.0 31 2.0 32 2.5 33 3.5 34 6.5 35 13.0 36 19.5 37 28.5 38 33.0 39 45.5 40 49.5 41 33.5 42 33.5 43 42.5 44 54.5 45 64.0 46 71.0 47 77.5 48 72.0 49 88.5 50 130.5 51 169.5 52 186.0 53 238.5 54 364.0 55 441.0 56 405.0 57 284.5 58 204.0 59 189.0 60 156.0 61 123.0 62 91.5 63 51.5 64 25.5 65 18.0 66 15.5 67 18.0 68 23.5 69 22.5 70 16.0 71 18.0 72 16.0 73 8.0 74 7.0 75 6.0 76 6.0 77 6.0 78 3.5 79 1.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.775 2 0.375 3 0.17500000000000002 4 0.575 5 0.325 6 0.42500000000000004 7 0.375 8 0.22499999999999998 9 0.2 10-14 0.28500000000000003 15-19 0.365 20-24 0.265 25-29 0.27499999999999997 30-34 0.215 35-39 0.28500000000000003 40-44 0.27499999999999997 45-49 0.27499999999999997 50-54 0.27 55-59 0.27999999999999997 60-64 0.25 65-69 0.26 70-74 0.27499999999999997 75-79 0.29 80-84 0.245 85-89 0.22499999999999998 90-94 0.23500000000000001 95-99 0.185 100-104 0.395 105-109 0.59 110-114 0.49 115-119 0.43499999999999994 120-124 0.63 125-129 0.365 130-134 0.45999999999999996 135-139 0.575 140-144 0.72 145-149 0.54 150-151 0.4875 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.25000000000001 #Duplication Level Percentage of deduplicated Percentage of total 1 73.855421686747 45.975 2 14.176706827309237 17.65 3 5.622489959839357 10.5 4 2.4096385542168677 6.0 5 1.6064257028112447 5.0 6 0.7630522088353414 2.85 7 0.1606425702811245 0.7000000000000001 8 0.5220883534136547 2.6 9 0.1606425702811245 0.8999999999999999 >10 0.6827309236947792 6.05 >50 0.040160642570281124 1.775 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTTCGATCCTGGCTCAGGATGAACGCTGGCGGCATGCTTAACACATGCAA 71 1.775 No Hit CCTTACCAGGGCTTGACATGCCGCGAATCCTCTTGAAAGAGAGGGGTGCC 27 0.675 No Hit CCTGCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGCAA 22 0.5499999999999999 No Hit GGCTTGCGGTGGATACCTAGGCACCCAGAGACGAGGAAGGGCGTAGCAAG 21 0.525 No Hit CTTACCAGGGCTTGACATGCCGCGAATCCTCTTGAAAGAGAGGGGTGCCC 15 0.375 No Hit GAACCTTACCAGGGCTTGACATGCCGCGAATCCTCTTGAAAGAGAGGGGT 14 0.35000000000000003 No Hit GCCTGACGGAGCAATGCCGCGTGGAGGTGGAAGGCCTACGGGTCGTCAAC 14 0.35000000000000003 No Hit CATGCCGCGAATCCTCTTGAAAGAGAGGGGTGCCCTCGGGAACGCGGACA 14 0.35000000000000003 No Hit GATGAACGCTGGCGGCATGCTTAACACATGCAAGTCGAACGGGAAGTGGT 14 0.35000000000000003 No Hit GCCGCGAATCCTCTTGAAAGAGAGGGGTGCCCTCGGGAACGCGGACACAG 13 0.325 No Hit GTTAAGTCTCGCAACGAGCGCAACCCTCGTGTTTAGTTGCCACTATGAGT 12 0.3 No Hit CTTACCAGGTCCAGACATAGCAAGGATTGACAGACTGAGAGCTCTTTCTT 12 0.3 No Hit AGTGACCAGGCCCGGGCGACTGTTTACCAAAAACACAGGTCTCCGCAAAG 12 0.3 No Hit CGGACACAGGTGGTGCATGGCTGTCGTCAGCTCGTGCCGTAAGGTGTTGG 11 0.27499999999999997 No Hit CAGTGACCAGGCCCGGGCGACTGTTTACCAAAAACACAGGTCTCCGCAAA 11 0.27499999999999997 No Hit CTTGCGGTGGATACCTAGGCACCCAGAGACGAGGAAGGGCGTAGCAAGCG 10 0.25 No Hit GTTGGGTTAAGTCTCGCAACGAGCGCAACCCTCGTGTTTAGTTGCCACTA 10 0.25 No Hit AGAACCTTACCAGGGCTTGACATGCCGCGAATCCTCTTGAAAGAGAGGGG 10 0.25 No Hit CCTGGCTCAGGATGAACGCTGGCGGCATGCTTAACACATGCAAGTCGAAC 9 0.22499999999999998 No Hit GGCGGGACTACCCGCTGAGTTTAAGCATATAAATAAGCGGAGGAGAAGAA 9 0.22499999999999998 No Hit CCCAAATAGGTCAACCTTTTAAACTGCCTGCTGAATCCATGAGCAGGCAA 9 0.22499999999999998 No Hit AGGAGGGGCTCGCGTCTGATTAGCTAGTTGGTGAGGCAATAGCTTACCAA 9 0.22499999999999998 No Hit GGGAAGCAACCGCGAAAGCGGGGGTCGACGAAGCGGAAGCGAGAATGTCG 8 0.2 No Hit GGAGGGGCTCGCGTCTGATTAGCTAGTTGGTGAGGCAATAGCTTACCAAG 8 0.2 No Hit GAACGCTGGCGGCATGCTTAACACATGCAAGTCGAACGGGAAGTGGTGTT 8 0.2 No Hit GCTTGCGGTGGATACCTAGGCACCCAGAGACGAGGAAGGGCGTAGCAAGC 8 0.2 No Hit GGATTAGAGACCCCAGTAGTCCTAGCCGTAAACGATGGATACTAGGTGCT 8 0.2 No Hit GGCGAAAGCCTGACGGAGCAATGCCGCGTGGAGGTGGAAGGCCTACGGGT 8 0.2 No Hit GCTTGACATGCCGCGAATCCTCTTGAAAGAGAGGGGTGCCCTCGGGAACG 8 0.2 No Hit GGGTGATCTATCCATGACCAGGATGAAGCTTGGATGAAACTAAGCAGAGG 8 0.2 No Hit GGTAGGGGCAGAGGGAATTTCCGGTGGAGCGGTGAAATGCATTGAGATCG 8 0.2 No Hit CACACGTGCTACAATGGGCGGGACAAAGGGTCGCGATCTCGCGAGGGTGA 8 0.2 No Hit CGAAAACATTGGTGAGAATCCAATGCCCCGAAAACCCAAGGTTTCCTCCG 8 0.2 No Hit CCTGAACAGACCGCCGGTGTTAAGCCGGAGGAAGGAGAGGATGAGGCCAA 8 0.2 No Hit GAAACAATGACGGTATCTGAGGAATAAGCATCGGCTAACTCTGTGCCAGC 8 0.2 No Hit GGAACAACAACTGGAAACGGTTGCTAATACCCCGTAGGCTGAGGAGCAAA 7 0.17500000000000002 No Hit GTGAAATTCTTGGATTTATGAAAGACGAACAACTGCGAAAGCATTTGCCA 7 0.17500000000000002 No Hit CTGACACTGAGAGACGAAAGCTAGGGGAGCAAATGGGATTAGAGACCCCA 7 0.17500000000000002 No Hit AGAGTTCGATCCTGGCTCAGGATGAACGCTGGCGGCATGCTTAACACATG 7 0.17500000000000002 No Hit GGCTGTCGTCAGCTCGTGCCGTAAGGTGTTGGGTTAAGTCTCGCAACGAG 6 0.15 No Hit ATGGGATTAGAGACCCCAGTAGTCCTAGCCGTAAACGATGGATACTAGGT 6 0.15 No Hit GCACTGTTTCGGTGCGGGCTGCGCGAGCGGTACCAAATCGAGGCAAACTC 6 0.15 No Hit GGAAAGAACACCAACGGCGAAAGCACTCTGCTGGGCCGACACTGACACTG 6 0.15 No Hit GTTGAAGAATGAGCCGGCGACTCATAGGCAGTGGCTTGGTTAAGGGAACG 6 0.15 No Hit GTCAGGCGGGACTACCCGCTGAGTTTAAGCATATAAATAAGCGGAGGAGA 6 0.15 No Hit CCTAAACCGTGAAAACGGGGTTGTGGGAGAGCAATACAAGCGTTGTGCTG 6 0.15 No Hit CTGAGGAATAAGCATCGGCTAACTCTGTGCCAGCAGCCGCGGTAAGACAG 6 0.15 No Hit ACTAGGTGCTGTGCGACTCGACCCGTGCAGTGCTGTAGCTAACGCGTTAA 6 0.15 No Hit CTCGGGAACGCGGACACAGGTGGTGCATGGCTGTCGTCAGCTCGTGCCGT 6 0.15 No Hit CAATAGCTTACCAAGGCGATGATCAGTAGCTGGTCCGAGAGGATGATCAG 6 0.15 No Hit GGCAATAGCTTACCAAGGCGATGATCAGTAGCTGGTCCGAGAGGATGATC 6 0.15 No Hit GATCCTGGCTCAGGATGAACGCTGGCGGCATGCTTAACACATGCAAGTCG 6 0.15 No Hit GTCGGCTTGAGTAACGAAAACATTGGTGAGAATCCAATGCCCCGAAAACC 6 0.15 No Hit CCCAAGCAGTGGGAGGGGAAAGTGATCTCTGACCGCGTGCCTGTTGAAGA 6 0.15 No Hit GAGTTCGATCCTGGCTCAGGATGAACGCTGGCGGCATGCTTAACACATGC 6 0.15 No Hit GTCTGATTAGCTAGTTGGTGAGGCAATAGCTTACCAAGGCGATGATCAGT 6 0.15 No Hit GGCTTTTCAAGTCCGCCGTCAAATCCCAGGGCTCAACCCTGGACAGGCGG 6 0.15 No Hit GGAGCAAATGGGATTAGAGACCCCAGTAGTCCTAGCCGTAAACGATGGAT 6 0.15 No Hit GTAACGCGTAAGAACCTGCCCTTGGGAGGGGAACAACAACTGGAAACGGT 5 0.125 No Hit GGGAAACAGCCCGGATCACCAGCTAAGGCCCCTAAATGACCGCTCAGTGA 5 0.125 No Hit GGACAAAGGGTCGCGATCTCGCGAGGGTGAGCTAACTCCAAAAACCCGTC 5 0.125 No Hit CACTGACACTGAGAGACGAAAGCTAGGGGAGCAAATGGGATTAGAGACCC 5 0.125 No Hit AGTCATCATGCCCCTTATGCCCTGGGCGACACACGTGCTACAATGGGCGG 5 0.125 No Hit GGATGAACGCTGGCGGCATGCTTAACACATGCAAGTCGAACGGGAAGTGG 5 0.125 No Hit CAACAACTGGAAACGGTTGCTAATACCCCGTAGGCTGAGGAGCAAAAGGA 5 0.125 No Hit CCTCTTGAAAGAGAGGGGTGCCCTCGGGAACGCGGACACAGGTGGTGCAT 5 0.125 No Hit AGCGTCTGTAGGTGGCTTTTCAAGTCCGCCGTCAAATCCCAGGGCTCAAC 5 0.125 No Hit GGTGTTTCCAGTGGCGAACGGGTGAGTAACGCGTAAGAACCTGCCCTTGG 5 0.125 No Hit GGCTTGACATGCCGCGAATCCTCTTGAAAGAGAGGGGTGCCCTCGGGAAC 5 0.125 No Hit GTTTAAGGACACAAGGTGACCCTGCTTTTTCAGGGTAAGAAGGGGTAGAG 5 0.125 No Hit GAAAGCATCACTAGCTTACGCTCTGACCCGAGTAGCATGGGGCACGTGGA 5 0.125 No Hit GTGAAATGCCACTCGAACCCAGAGCTAGCTGGTTCTCCCCGAAATGCGTT 5 0.125 No Hit GAGAAATCCGCCCAAGGAGGGGCTCGCGTCTGATTAGCTAGTTGGTGAGG 5 0.125 No Hit GCATCGGCTAACTCTGTGCCAGCAGCCGCGGTAAGACAGAGGATGCAAGC 5 0.125 No Hit GTCGAACGGGAAGTGGTGTTTCCAGTGGCGAACGGGTGAGTAACGCGTAA 5 0.125 No Hit GGAAGGCCTACGGGTCGTCAACTTCTTTTCTCGGAGAAGAAACAATGACG 5 0.125 No Hit AGCAAAAGGAGAAATCCGCCCAAGGAGGGGCTCGCGTCTGATTAGCTAGT 5 0.125 No Hit AGGGAATTTCCGGTGGAGCGGTGAAATGCATTGAGATCGGAAAGAACACC 5 0.125 No Hit GGGGTAGAGAAAATGCCTCGAGCCGAGGTCCGAGTACCAAGCGCTGCAGC 5 0.125 No Hit CGAGAATGTCGGCTTGAGTAACGAAAACATTGGTGAGAATCCAATGCCCC 5 0.125 No Hit GACAGGTTAGTTTTACCCTACTGATGACCGTGCCGCGATAGTAATTCAAC 5 0.125 No Hit GACAAAGGGTCGCGATCTCGCGAGGGTGAGCTAACTCCAAAAACCCGTCC 5 0.125 No Hit AAGACAGCCAGGAGGTTTGCCTAGAAGCAGCCACCCTTTAAAGAGTGCGT 5 0.125 No Hit GCCTAAACCGTGAAAACGGGGTTGTGGGAGAGCAATACAAGCGTTGTGCT 5 0.125 No Hit CTCGTGTTTAGTTGCCACTATGAGTTTGGAACCCTGAACAGACCGCCGGT 5 0.125 No Hit ACCTTACCAGGGCTTGACATGCCGCGAATCCTCTTGAAAGAGAGGGGTGC 5 0.125 No Hit CATGAGCAGGCAAGAGACAACCTGGCGAACTGAAACATCTTAGTAGCCAG 5 0.125 No Hit CCTAGGCACCCAGAGACGAGGAAGGGCGTAGCAAGCGACGAAATGCTTCG 5 0.125 No Hit CGGCAACGGATATCTCGGCTCTCGCATCGATGAAGAACGTAGCGAAATGC 5 0.125 No Hit GAAGAAACTTACAAGGATTCCCCTAGTAACGGCGAGCGAACCGGGAGCAG 5 0.125 No Hit GGGAGAGCAATACAAGCGTTGTGCTGCTAGGCGAAGCGGTTGAGTGCCGC 5 0.125 No Hit CGGGTGAGTAACGCGTAAGAACCTGCCCTTGGGAGGGGAACAACAACTGG 5 0.125 No Hit CGATCCTGGCTCAGGATGAACGCTGGCGGCATGCTTAACACATGCAAGTC 5 0.125 No Hit CGGGAACGCGGACACAGGTGGTGCATGGCTGTCGTCAGCTCGTGCCGTAA 5 0.125 No Hit CCTGACGGAGCAATGCCGCGTGGAGGTGGAAGGCCTACGGGTCGTCAACT 5 0.125 No Hit GGCCTACCATGGTGGTGACGGGTGACGGAGAATTAGGGTTCGATTCCGGA 5 0.125 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 5 0.125 No Hit GTCAGCTCGTGCCGTAAGGTGTTGGGTTAAGTCTCGCAACGAGCGCAACC 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.025 0.0 0.0 0.0 0.0 30-31 0.025 0.0 0.0 0.0 0.0 32-33 0.025 0.0 0.0 0.0 0.0 34-35 0.037500000000000006 0.0 0.0 0.0 0.0 36-37 0.05 0.0 0.0 0.0 0.0 38-39 0.075 0.0 0.0 0.0 0.0 40-41 0.075 0.0 0.0 0.0 0.0 42-43 0.075 0.0 0.0 0.0 0.0 44-45 0.1 0.0 0.0 0.0 0.0 46-47 0.125 0.0 0.0 0.0 0.0 48-49 0.1875 0.0 0.0 0.0 0.0 50-51 0.225 0.0 0.0 0.0 0.0 52-53 0.4 0.0 0.0 0.0 0.0 54-55 0.5 0.0 0.0 0.0 0.0 56-57 0.575 0.0 0.0 0.0 0.0 58-59 0.75 0.0 0.0 0.0 0.0 60-61 0.8875 0.0 0.0 0.0 0.0 62-63 1.1 0.0 0.0 0.0 0.0 64-65 1.2875 0.0 0.0 0.0 0.0 66-67 1.525 0.0 0.0 0.0 0.0 68-69 1.8125 0.0 0.0 0.0 0.0 70-71 2.1624999999999996 0.0 0.0 0.0 0.0 72-73 2.5999999999999996 0.0 0.0 0.0 0.0 74-75 3.325 0.0 0.0 0.0 0.0 76-77 3.9749999999999996 0.0 0.0 0.0 0.0 78-79 4.775 0.0 0.0 0.0 0.0 80-81 5.7125 0.0 0.0 0.0 0.0 82-83 6.65 0.0 0.0 0.0 0.0 84-85 7.45 0.0 0.0 0.0 0.0 86-87 8.7875 0.0 0.0 0.0 0.0 88-89 10.05 0.0 0.0 0.0 0.0 90-91 11.3625 0.0 0.0 0.0 0.0 92-93 12.5375 0.0 0.0 0.0 0.0 94-95 13.775 0.0 0.0 0.0 0.0 96-97 15.100000000000001 0.0 0.0 0.0 0.0 98-99 16.5875 0.0 0.0 0.0 0.0 100-101 17.887500000000003 0.0 0.0 0.0 0.0 102-103 19.175 0.0 0.0 0.0 0.0 104-105 20.7375 0.0 0.0 0.0 0.0 106-107 22.0 0.0 0.0 0.0 0.0 108-109 23.3625 0.0 0.0 0.0 0.0 110-111 24.6125 0.0 0.0 0.0 0.0 112-113 26.2125 0.0 0.0 0.0 0.0 114-115 27.6375 0.0 0.0 0.0 0.0 116-117 29.375 0.0 0.0 0.0 0.0 118-119 30.6625 0.0 0.0 0.0 0.0 120-121 31.75 0.0 0.0 0.0 0.0 122-123 32.8625 0.0 0.0 0.0 0.0 124-125 34.1375 0.0 0.0 0.0 0.0 126-127 35.3 0.0 0.0 0.0 0.0 128-129 36.5375 0.0 0.0 0.0 0.0 130-131 37.625 0.0 0.0 0.0 0.0 132-133 38.625 0.0 0.0 0.0 0.0 134-135 39.575 0.0 0.0 0.0 0.0 136-137 40.625 0.0 0.0 0.0 0.0 138-139 41.55 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AACCGCG 10 0.006830828 145.0 8 CAACCGC 10 0.006830828 145.0 7 GAAGCAA 10 0.006830828 145.0 3 ACCGCGA 10 0.006830828 145.0 9 GCAACCG 10 0.006830828 145.0 6 GGAAGCA 10 0.006830828 145.0 2 AGCAACC 10 0.006830828 145.0 5 AGTTTGA 10 0.006830828 145.0 4 AAGCAAC 10 0.006830828 145.0 4 TAGATCT 90 0.0032161705 32.22222 145 >>END_MODULE Read 1500687 spots for SRR6941554.sra Written 1500687 spots for SRR6941554.sra Read 1500687 spots for SRR6941554.sra Written 1500687 spots for SRR6941554.sra Read 1500687 spots for SRR6941554.sra Written 1500687 spots for SRR6941554.sra Read 1500687 spots for SRR6941554.sra Written 1500687 spots for SRR6941554.sra Read 1500687 spots for SRR6941554.sra Written 1500687 spots for SRR6941554.sra Read 1500687 spots for SRR6941554.sra Written 1500687 spots for SRR6941554.sra Read 1500687 spots for SRR6941554.sra Written 1500687 spots for SRR6941554.sra Read 1500687 spots for SRR6941554.sra Written 1500687 spots for SRR6941554.sra Read 1500687 spots for SRR6941554.sra Written 1500687 spots for SRR6941554.sra Read 1500687 spots for SRR6941554.sra Written 1500687 spots for SRR6941554.sra Read 1500687 spots for SRR6941554.sra Written 1500687 spots for SRR6941554.sra Read 1500687 spots for SRR6941554.sra Written 1500687 spots for SRR6941554.sra Read 1500687 spots for SRR6941554.sra Written 1500687 spots for SRR6941554.sra Read 1500687 spots for SRR6941554.sra Written 1500687 spots for SRR6941554.sra Read 1500687 spots for SRR6941554.sra Written 1500687 spots for SRR6941554.sra Read 1500699 spots for SRR6941554.sra Written 1500699 spots for SRR6941554.sra Read 1500687 spots for SRR6941554.sra Written 1500687 spots for SRR6941554.sra Read 1500687 spots for SRR6941554.sra Written 1500687 spots for SRR6941554.sra Read 1500687 spots for SRR6941554.sra Written 1500687 spots for SRR6941554.sra Read 1500687 spots for SRR6941554.sra Written 1500687 spots for SRR6941554.sra SRR ids: ['SRR6941554.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_cuiul_od SRR6941554.sra spots: 30013752 blocks: [[1, 1500687], [1500688, 3001374], [3001375, 4502061], [4502062, 6002748], [6002749, 7503435], [7503436, 9004122], [9004123, 10504809], [10504810, 12005496], [12005497, 13506183], [13506184, 15006870], [15006871, 16507557], [16507558, 18008244], [18008245, 19508931], [19508932, 21009618], [21009619, 22510305], [22510306, 24010992], [24010993, 25511679], [25511680, 27012366], [27012367, 28513053], [28513054, 30013752]] SRR6941554 file size 10148975 SRR6941554 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR6941554 SRR6941554_1.fastq SRR6941554_2.fastq Input file: SRR6941554_1.fastq Paired file: SRR6941554_2.fastq trimmed: SRR6941554-trimmed-pair1.fastq, SRR6941554-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Fri Dec 6 11:15:28 2024 >> started Fri Dec 6 11:21:10 2024 >> done (342.395s) 30013752 read pairs processed; of these: 11119 ( 0.04%) short read pairs filtered out after trimming by size control 37356 ( 0.12%) empty read pairs filtered out after trimming by size control 29965277 (99.84%) read pairs available; of these: 17658458 (58.93%) trimmed read pairs available after processing 12306819 (41.07%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 53 0.00% 19 96 0.00% 20 106 0.00% 21 135 0.00% 22 178 0.00% 23 171 0.00% 24 265 0.00% 25 299 0.00% 26 547 0.00% 27 628 0.00% 28 809 0.00% 29 1108 0.00% 30 1448 0.00% 31 1516 0.01% 32 1873 0.01% 33 2116 0.01% 34 2493 0.01% 35 2805 0.01% 36 2884 0.01% 37 2946 0.01% 38 3443 0.01% 39 4376 0.01% 40 4747 0.02% 41 5365 0.02% 42 5452 0.02% 43 5643 0.02% 44 6432 0.02% 45 6384 0.02% 46 6786 0.02% 47 7375 0.02% 48 8600 0.03% 49 10242 0.03% 50 10970 0.04% 51 12073 0.04% 52 14543 0.05% 53 15278 0.05% 54 17006 0.06% 55 15852 0.05% 56 17356 0.06% 57 19098 0.06% 58 23425 0.08% 59 24094 0.08% 60 25811 0.09% 61 34093 0.11% 62 37959 0.13% 63 36663 0.12% 64 39961 0.13% 65 44266 0.15% 66 43796 0.15% 67 45455 0.15% 68 56244 0.19% 69 64773 0.22% 70 68110 0.23% 71 67604 0.23% 72 87276 0.29% 73 94219 0.31% 74 84211 0.28% 75 90852 0.30% 76 98567 0.33% 77 108543 0.36% 78 102823 0.34% 79 117786 0.39% 80 125866 0.42% 81 130940 0.44% 82 139093 0.46% 83 147429 0.49% 84 149640 0.50% 85 179170 0.60% 86 203537 0.68% 87 191105 0.64% 88 205664 0.69% 89 176890 0.59% 90 177363 0.59% 91 194360 0.65% 92 200864 0.67% 93 220799 0.74% 94 214434 0.72% 95 207167 0.69% 96 201317 0.67% 97 209644 0.70% 98 202348 0.68% 99 202012 0.67% 100 204878 0.68% 101 214750 0.72% 102 227545 0.76% 103 218730 0.73% 104 237250 0.79% 105 221271 0.74% 106 211657 0.71% 107 219405 0.73% 108 213272 0.71% 109 254903 0.85% 110 225212 0.75% 111 232835 0.78% 112 239019 0.80% 113 213141 0.71% 114 216735 0.72% 115 219072 0.73% 116 203755 0.68% 117 206321 0.69% 118 192797 0.64% 119 184943 0.62% 120 204196 0.68% 121 179841 0.60% 122 179041 0.60% 123 229899 0.77% 124 207068 0.69% 125 212668 0.71% 126 181894 0.61% 127 178094 0.59% 128 182526 0.61% 129 175321 0.59% 130 171058 0.57% 131 176602 0.59% 132 179362 0.60% 133 178485 0.60% 134 183117 0.61% 135 189661 0.63% 136 200640 0.67% 137 195048 0.65% 138 190794 0.64% 139 183187 0.61% 140 171393 0.57% 141 177471 0.59% 142 202997 0.68% 143 202580 0.68% 144 199118 0.66% 145 209380 0.70% 146 215637 0.72% 147 233883 0.78% 148 249501 0.83% 149 323063 1.08% 150 2041772 6.81% 151 12306819 41.07% 29965277 reads passed initial QC criterion=sequence-density sequence-density=3.91 sequence-density-rank=1 fanout-score=1.96 fanout-score-rank=31 prefix-density=3.86 prefix-fanout=2.0 sequence=GTATTTAGCCTTG criterion=fanout-score sequence-density=0.03 sequence-density-rank=32 fanout-score=47.06 fanout-score-rank=1 prefix-density=1.28 prefix-fanout=1.0 sequence=GCTTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCATTAATAGCGGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTACGTTCGTGCATTACTTCCATACCAAGATTAGCACGGTTGATGATATCAGCCCAAGTATTAATAACGCGACCTTGACTATCAACTACAGATTGGTTGAAATTGAATCCATTTAGGTTGAACGCCATAGTACTAATACCTAAAGCAGTGAACCAGATTCCTACTACAGGCCAAGCAGCCAAGAAGAAGTGTAAAGAACGAGAGTTGTTGAAACTAGCATATTGGAAGATTAATCGGCCAAAATAACCATGAGCAGCCACAATATTATAAGTCTCTTCCTCTTGACCAAATTTGTAACCCTCATTAGCAGATTCATTTTCAGTAGTTTCCCTGATCAAACTAGAGGTTACCAAGGAACCATGCATAGCACTGAATAGGGAACCGCCGAAAACACCAGCTACACCTAACAT criterion=sequence-density sequence-density=1.31 sequence-density-rank=1 fanout-score=2.05 fanout-score-rank=30 prefix-density=1.33 prefix-fanout=2.0 sequence=CCTAGTACGAGAGGA criterion=fanout-score sequence-density=0.03 sequence-density-rank=31 fanout-score=88.19 fanout-score-rank=1 prefix-density=1.96 prefix-fanout=1.6 sequence=GGGCGAAAGCCCGATCCAGCAATATCGCGTGAGTGAAGAAGGGCAATGCCGCTTGTAAAGCTCTTTCGTCGAGTGCGCGATCATGACAGGACTCGAGGAAGAAGCCCCGGCTAACTCCGTGCCAGCAGC Potential 3prime adapter identified. Now checking if in reference sequence /dee2/code/volunteer_pipeline.sh: line 905: -f: command not found /dee2/code/volunteer_pipeline.sh: line 906: -f: command not found Adapter seq not found in reference. Now shuffling file before clipping skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -t 20 -x GTATTTAGCCTTG -y CCTAGTACGAGAGGA -o SRR6941554 SRR6941554_1.fastq SRR6941554_2.fastq Input file: SRR6941554_1.fastq Paired file: SRR6941554_2.fastq trimmed: SRR6941554-trimmed-pair1.fastq, SRR6941554-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): GTATTTAGCCTTG -- paired 3' end adapter sequence (-y): CCTAGTACGAGAGGA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Fri Dec 6 11:36:52 2024 >> started Fri Dec 6 11:38:11 2024 >> done (78.738s) 9988426 read pairs processed; of these: 725 ( 0.01%) short read pairs filtered out after trimming by size control 1632 ( 0.02%) empty read pairs filtered out after trimming by size control 9986069 (99.98%) read pairs available; of these: 766 ( 0.01%) trimmed read pairs available after processing 9985303 (99.99%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 17 0.00% 19 40 0.00% 20 37 0.00% 21 44 0.00% 22 61 0.00% 23 54 0.00% 24 91 0.00% 25 98 0.00% 26 191 0.00% 27 203 0.00% 28 250 0.00% 29 363 0.00% 30 494 0.00% 31 506 0.01% 32 643 0.01% 33 720 0.01% 34 826 0.01% 35 943 0.01% 36 994 0.01% 37 990 0.01% 38 1144 0.01% 39 1440 0.01% 40 1516 0.02% 41 1758 0.02% 42 1775 0.02% 43 1869 0.02% 44 2159 0.02% 45 2094 0.02% 46 2224 0.02% 47 2463 0.02% 48 2847 0.03% 49 3294 0.03% 50 3644 0.04% 51 4028 0.04% 52 4829 0.05% 53 5047 0.05% 54 5644 0.06% 55 5250 0.05% 56 5829 0.06% 57 6380 0.06% 58 7850 0.08% 59 8065 0.08% 60 8618 0.09% 61 11368 0.11% 62 12540 0.13% 63 12177 0.12% 64 13316 0.13% 65 14707 0.15% 66 14585 0.15% 67 15235 0.15% 68 18835 0.19% 69 21479 0.22% 70 22890 0.23% 71 22746 0.23% 72 29276 0.29% 73 31463 0.32% 74 28091 0.28% 75 30161 0.30% 76 32662 0.33% 77 36383 0.36% 78 33862 0.34% 79 38972 0.39% 80 41813 0.42% 81 43342 0.43% 82 46267 0.46% 83 49099 0.49% 84 50031 0.50% 85 59757 0.60% 86 67926 0.68% 87 63689 0.64% 88 68422 0.69% 89 59131 0.59% 90 59400 0.59% 91 64818 0.65% 92 66997 0.67% 93 73671 0.74% 94 71147 0.71% 95 69355 0.69% 96 67291 0.67% 97 70061 0.70% 98 67420 0.68% 99 67053 0.67% 100 68432 0.69% 101 71280 0.71% 102 75621 0.76% 103 72927 0.73% 104 79275 0.79% 105 73647 0.74% 106 70703 0.71% 107 73293 0.73% 108 71659 0.72% 109 84533 0.85% 110 75120 0.75% 111 77167 0.77% 112 79674 0.80% 113 70669 0.71% 114 72094 0.72% 115 73053 0.73% 116 67919 0.68% 117 68926 0.69% 118 64190 0.64% 119 61540 0.62% 120 68010 0.68% 121 60029 0.60% 122 59765 0.60% 123 76791 0.77% 124 68902 0.69% 125 70788 0.71% 126 60628 0.61% 127 59350 0.59% 128 60964 0.61% 129 58402 0.58% 130 56713 0.57% 131 59038 0.59% 132 59744 0.60% 133 59317 0.59% 134 61041 0.61% 135 63147 0.63% 136 66697 0.67% 137 64648 0.65% 138 63405 0.63% 139 61362 0.61% 140 56978 0.57% 141 59134 0.59% 142 67716 0.68% 143 67365 0.67% 144 66295 0.66% 145 69808 0.70% 146 72025 0.72% 147 78123 0.78% 148 82905 0.83% 149 107932 1.08% 150 680315 6.81% 151 4102240 41.08% criterion=sequence-density sequence-density=3.88 sequence-density-rank=1 fanout-score=1.96 fanout-score-rank=30 prefix-density=3.84 prefix-fanout=2.0 sequence=GTATTTAGCCTTG criterion=fanout-score sequence-density=0.02 sequence-density-rank=33 fanout-score=52.38 fanout-score-rank=1 prefix-density=1.30 prefix-fanout=1.0 sequence=GCTTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCATTAATAGCGGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTACGTTCGTGCATTACTTCCATACCAAGATTAGCACGGTTGATGATATCAGCCCAAGTATTAATAACGCGACCTTGACTATCAACTACAGATTGGTTGAAATTGAATCCATTTAGGTTGAACGCCATAGTACTAATACCTAAAGCAGTGAACCAGATTCCTACTACAGGCCAAGCAGCCAAGAAGAAGTGTAAAGAACGAGAGTTGTTGAAACTAGCATATTGGAAGATTAATCGGCCAAAATAACCATGAGCAGCCACAATATTATAAGTCTCTTCCTCTTGACCAAATTTGTAACCCTCATTAGCAGATTCATTTTCAGTAGTTTCCCTGATCAAACTAGAGGTTACCAAGGAACCATGCATAGCACTGAATAGGGAACCGCCGAAAACACCAGCTACACCTAACAT criterion=sequence-density sequence-density=1.30 sequence-density-rank=1 fanout-score=2.06 fanout-score-rank=29 prefix-density=1.32 prefix-fanout=2.0 sequence=CCTAGTACGAGAGGA criterion=fanout-score sequence-density=0.02 sequence-density-rank=33 fanout-score=63.01 fanout-score-rank=1 prefix-density=1.43 prefix-fanout=1.1 sequence=AGAAGGGGTGCCCCCTCACAAAAGGGGGTCGCAGTGACCAGGCCCGGGCGACTGTTTACCAAAAACACAGGTCTCCGCAAAGTCGTAAGACCATGTATGGGGGCTGACGCCTGCCCAGTGCCGGAAGGTCAAGGAAGTTGGTGAACTGATGACAGGGAAGCCGGCGACCGAAGCCCCGGTGAACGGCGGCCGTAAC SRR6941554 testing PE reads STAR mapping to Ensembl genome Started job on | Dec 06 11:46:11 Started mapping on | Dec 06 11:46:13 Finished on | Dec 06 12:23:22 Mapping speed, Million of reads per hour | 48.39 Number of input reads | 29962920 Average input read length | 259 UNIQUE READS: Uniquely mapped reads number | 7040620 Uniquely mapped reads % | 23.50% Average mapped length | 278.25 Number of splices: Total | 937400 Number of splices: Annotated (sjdb) | 601965 Number of splices: GT/AG | 657444 Number of splices: GC/AG | 13009 Number of splices: AT/AC | 2971 Number of splices: Non-canonical | 263976 Mismatch rate per base, % | 0.09% Deletion rate per base | 0.00% Deletion average length | 1.79 Insertion rate per base | 0.00% Insertion average length | 1.80 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 14885583 % of reads mapped to multiple loci | 49.68% Number of reads mapped to too many loci | 2003495 % of reads mapped to too many loci | 6.69% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 3.13% % of reads unmapped: other | 17.01% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 8040509 8040509 8040509 N_multimapping 14885583 14885583 14885583 N_noFeature 5459324 6942476 5527233 N_ambiguous 75636 4029 43317 UnstrandedReadsAssigned:1505660 PositiveStrandReadsAssigned:94115 NegativeStrandReadsAssigned:1470070 Dataset is classified negative stranded MeadianReadLen=148 20thPercentileLength=103 echo kmer=99 SRR6941554 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,972 [index] number of k-mers: 66,720,672 [index] number of equivalence classes: 111,837 [quant] running in paired-end mode [quant] will process pair 1: SRR6941554-trimmed-pair1.fastq SRR6941554-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 29,962,920 reads, 5,663,485 reads pseudoaligned [quant] estimated average fragment length: 151.999 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 916 rounds 52973 SRR6941554.ke.tsv 35125 SRR6941554.se.tsv 88098 total ==> SRR6941554.ke.tsv <== target_id length eff_length est_counts tpm PNS24245 936 785.07 1.25127 0.0780166 PNS24247 1044 893.001 0 0 PNS24249 1928 1777 10.7487 0.296084 PNS24246 1044 893.001 0 0 PNS24248 1044 893.001 0 0 PNS24244 1471 1320 0 0 PNS24243 293 144.743 0 0 KQK14069 1603 1452 254.349 8.5745 KQK14071 474 323.361 26.2893 3.97957 ==> SRR6941554.se.tsv <== BRADI_1g14170v3 305 BRADI_1g53295v3 1 BRADI_1g59795v3 4 BRADI_1g07683v3 0 BRADI_1g00485v3 0 BRADI_1g20270v3 12 BRADI_1g74790v3 11 BRADI_1g09890v3 1 BRADI_1g77505v3 5 BRADI_1g48960v3 0 SRR6941554 completed mapping pipeline successfully