Starting /dee2/code/volunteer_pipeline.sh SRR6941555 current disk space = 1551421018112 free memory = 1604051316 SRR6941555 SRAfilesize c62627684d501161adf65375b16f282b SRR6941555.sra SRR6941555.sra file validated SRR6941555 is paired end SRR6941555 is conventional basespace SRR6941555 read1 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR6941555_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 52 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 34.47975 35.0 35.0 35.0 34.0 35.0 2 34.44975 35.0 35.0 35.0 33.0 35.0 3 34.577 35.0 35.0 35.0 34.0 35.0 4 34.64175 35.0 35.0 35.0 35.0 35.0 5 34.509 35.0 35.0 35.0 33.0 35.0 6 39.32325 40.0 40.0 40.0 39.0 40.0 7 39.2845 40.0 40.0 40.0 39.0 40.0 8 39.20475 40.0 40.0 40.0 38.0 40.0 9 39.368 40.0 40.0 40.0 39.0 40.0 10-14 39.361749999999994 40.0 40.0 40.0 39.0 40.0 15-19 39.17555 40.0 40.0 40.0 38.6 40.0 20-24 39.306200000000004 40.0 40.0 40.0 39.0 40.0 25-29 39.29215000000001 40.0 40.0 40.0 38.8 40.0 30-34 39.172450000000005 40.0 40.0 40.0 38.4 40.0 35-39 39.23745 40.0 40.0 40.0 38.8 40.0 40-44 39.2358 40.0 40.0 40.0 38.8 40.0 45-49 39.202 40.0 40.0 40.0 38.8 40.0 50-54 39.13085 40.0 40.0 40.0 38.4 40.0 55-59 39.18795000000001 40.0 40.0 40.0 38.4 40.0 60-64 39.114549999999994 40.0 40.0 40.0 38.0 40.0 65-69 39.127300000000005 40.0 40.0 40.0 38.0 40.0 70-74 39.0779 40.0 40.0 40.0 38.0 40.0 75-79 39.0168 40.0 39.4 40.0 37.8 40.0 80-84 39.046850000000006 40.0 39.2 40.0 38.0 40.0 85-89 38.8589 40.0 39.0 40.0 36.6 40.0 90-94 38.970150000000004 40.0 39.0 40.0 37.4 40.0 95-99 38.7877 40.0 39.0 40.0 36.4 40.0 100-104 38.20215 39.4 38.4 39.8 35.4 39.8 105-109 38.78785 40.0 39.0 40.0 36.6 40.0 110-114 38.82415 40.0 39.0 40.0 36.4 40.0 115-119 38.8309 40.0 39.0 40.0 36.4 40.0 120-124 38.7325 40.0 39.0 40.0 36.0 40.0 125-129 38.4549 40.0 39.0 40.0 35.8 40.0 130-134 38.427949999999996 40.0 39.0 40.0 35.8 40.0 135-139 38.27645 40.0 39.0 40.0 35.4 40.0 140-144 38.04845 40.0 39.0 40.0 34.8 40.0 145-149 37.49405 40.0 39.0 40.0 33.8 40.0 150-151 34.4865 38.5 35.0 39.5 18.0 40.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 23 2.0 24 5.0 25 2.0 26 6.0 27 12.0 28 15.0 29 14.0 30 25.0 31 40.0 32 45.0 33 46.0 34 85.0 35 101.0 36 115.0 37 203.0 38 374.0 39 2910.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 37.27158948685857 6.433041301627034 3.879849812265332 52.415519399249064 2 17.890253069406164 8.844901027311451 35.30443497870208 37.96041092458031 3 16.900000000000002 11.25 25.2 46.650000000000006 4 25.35 18.775 19.325 36.55 5 24.91844416562108 24.015056461731493 25.420326223337515 25.646173149309913 6 23.849999999999998 25.15 25.525 25.474999999999998 7 17.375 21.925 39.45 21.25 8 18.6 18.65 34.925 27.825 9 20.1 16.425 36.95 26.525 10-14 22.085 22.415 27.439999999999998 28.060000000000002 15-19 23.59 21.83 25.374999999999996 29.205 20-24 23.45 23.0 26.25 27.3 25-29 24.11 20.07 26.93 28.89 30-34 23.585 21.075 25.28 30.06 35-39 22.634999999999998 21.105 27.150000000000002 29.110000000000003 40-44 23.665 21.575 24.95 29.81 45-49 23.885 20.315 26.029999999999998 29.770000000000003 50-54 23.43 20.9 26.6 29.07 55-59 22.195 22.395 27.355 28.055000000000003 60-64 24.279999999999998 21.015 26.775 27.93 65-69 23.46 23.205000000000002 25.195 28.139999999999997 70-74 23.369999999999997 22.485 25.335 28.810000000000002 75-79 24.375 23.13 24.66 27.834999999999997 80-84 22.720000000000002 24.315 24.46 28.505000000000003 85-89 24.16 22.825 24.474999999999998 28.54 90-94 25.2 23.335 23.665 27.800000000000004 95-99 24.635 22.99 24.235 28.139999999999997 100-104 23.919999999999998 22.900000000000002 25.245 27.935 105-109 22.075 23.3 24.755 29.87 110-114 22.91 23.32 25.869999999999997 27.900000000000002 115-119 22.585 23.32 25.009999999999998 29.085 120-124 23.125 25.185000000000002 22.485 29.205 125-129 22.759999999999998 23.785 23.369999999999997 30.085 130-134 23.655 21.87 23.615 30.86 135-139 21.975 22.85 25.335 29.84 140-144 24.115000000000002 22.845 23.655 29.385 145-149 21.845 24.02 25.465 28.67 150-151 20.63611319809667 23.6038066616579 25.85775106436263 29.902329075882793 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 1.0 23 1.5 24 0.5 25 1.5 26 2.0 27 3.5 28 3.5 29 2.5 30 4.5 31 5.5 32 5.5 33 8.5 34 10.0 35 8.5 36 21.5 37 45.0 38 53.5 39 64.0 40 73.5 41 74.5 42 60.0 43 57.5 44 69.0 45 82.5 46 92.5 47 80.5 48 67.5 49 85.0 50 126.0 51 160.0 52 165.0 53 189.0 54 246.5 55 389.0 56 444.5 57 298.5 58 246.0 59 247.0 60 154.0 61 81.0 62 63.0 63 61.5 64 47.0 65 21.5 66 16.5 67 8.5 68 4.5 69 1.5 70 6.5 71 11.5 72 10.0 73 6.5 74 3.0 75 3.0 76 3.0 77 1.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.125 2 0.22499999999999998 3 0.0 4 0.0 5 0.375 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.17500000000000002 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.425000000000004 #Duplication Level Percentage of deduplicated Percentage of total 1 73.07171853856563 40.5 2 13.080739738385205 14.499999999999998 3 5.367613892647722 8.924999999999999 4 3.473161930536761 7.7 5 1.0374379792512405 2.875 6 0.9021199819576003 3.0 7 0.6765899864682002 2.625 8 0.4510599909788002 2.0 9 0.2706359945872801 1.35 >10 1.5787099684258006 13.275 >50 0.09021199819576003 3.25 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GGCATGATGACTTGGCCTCATCCTCTCCTTCCTCCGGCTTAACACCGGCG 66 1.6500000000000001 No Hit CCCCACTGCTGCCTCCCGTAGGAGTCTGGGCCGTGTCTCAGTCCCAGTGT 64 1.6 No Hit CCCACTGCTGCCTCCCGTAGGAGTCTGGGCCGTGTCTCAGTCCCAGTGTG 37 0.9249999999999999 No Hit CCGGGAACGGATTCACCGCCGTATGGCTGACCGGCGATTACTAGCGATTC 31 0.775 No Hit GTCCAGTCAACTGCTGCGCCTCAACGCATTTCGGGGAGAACCAGCTAGCT 30 0.75 No Hit CCCGTCAATTCCTTTGAGTTTCATTCTTGCGAACGTACTCCCCAGGCGGG 29 0.7250000000000001 No Hit CTCCACGCGGCATTGCTCCGTCAGGCTTTCGCCCATTGCGGAAAATTCCC 21 0.525 No Hit CCTCAGCCTACGGGGTATTAGCAACCGTTTCCAGTTGTTGTTCCCCTCCC 17 0.42500000000000004 No Hit GTTCGAGCTTTTCCTGGGAGTATGGCATCGGTTACATACTTCAGTGCCGT 17 0.42500000000000004 No Hit CCTAGATGTCCAGTCAACTGCTGCGCCTCAACGCATTTCGGGGAGAACCA 16 0.4 No Hit GTTCTATTTCACTACCCACTGGGGGTTCTTTTCACCTTTCCCTCACGGTA 16 0.4 No Hit GTGCTCTGCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTCCTA 16 0.4 No Hit CCTAGCTTTCGTCTCTCAGTGTCAGTGTCGGCCCAGCAGAGTGCTTTCGC 16 0.4 No Hit GTCGGTTTCGGGTACAGGTACCCTTTTGTTGAAGGTCGTTCGAGCTTTTC 15 0.375 No Hit CCGTCAATTCCTTTGAGTTTCATTCTTGCGAACGTACTCCCCAGGCGGGA 14 0.35000000000000003 No Hit CCATCGTTTACGGCTAGGACTACTGGGGTCTCTAATCCCATTTGCTCCCC 14 0.35000000000000003 No Hit GACCTGTTGTCCATCGACTACGCCTTTCGGCCTGATCTTAGGCCCTGACT 14 0.35000000000000003 No Hit CACCTCCACGCGGCATTGCTCCGTCAGGCTTTCGCCCATTGCGGAAAATT 14 0.35000000000000003 No Hit CTCAGATACCGTCATTGTTTCTTCTCCGAGAAAAGAAGTTGACGACCCGT 14 0.35000000000000003 No Hit GTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTC 13 0.325 No Hit CCTGTGTCGGTTTCGGGTACAGGTACCCTTTTGTTGAAGGTCGTTCGAGC 13 0.325 No Hit GGGCATGATGACTTGGCCTCATCCTCTCCTTCCTCCGGCTTAACACCGGC 12 0.3 No Hit GTCCCTATTAATCATTACTCCGATCCCGAAGGCCAACACAATAGGACCGG 12 0.3 No Hit CATCGTTTACGGCTAGGACTACTGGGGTCTCTAATCCCATTTGCTCCCCT 12 0.3 No Hit CCCTAGAGTAACTTTTATCCGTTGAGCGACGGCCCTTCCACTCGGCACCG 12 0.3 No Hit CGTCAATTCCTTTGAGTTTCATTCTTGCGAACGTACTCCCCAGGCGGGAT 11 0.27499999999999997 No Hit GTCAATTCCTTTGAGTTTCATTCTTGCGAACGTACTCCCCAGGCGGGATA 11 0.27499999999999997 No Hit CTCCCATTTCGCTCGCCGCTACTACGGGAATCGCTTTTGCTTTCTTTTCC 11 0.27499999999999997 No Hit CCCTACCGTACTCCAGCTTGGTAGTTTCCACCGCCTGTCCAGGGTTGAGC 11 0.27499999999999997 No Hit CTCCTTTTGCTCCTCAGCCTACGGGGTATTAGCAACCGTTTCCAGTTGTT 11 0.27499999999999997 No Hit GCCCAATCATTCCGGATAACGCTTGCATCCTCTGTCTTACCGCGGCTGCT 11 0.27499999999999997 No Hit CTTGCAAGGTGGTCCTTGCTGATTCACACGGGATTCCACGTGCCCCATGC 10 0.25 No Hit CTTAAACCTATAACCATCTTTCGGCTAACCTAGCCTCCTCCGTCCCTCCG 10 0.25 No Hit GTCCCAGTGTGGCTGATCATCCTCTCGGACCAGCTACTGATCATCGCCTT 10 0.25 No Hit CTGTTGTCCATCGACTACGCCTTTCGGCCTGATCTTAGGCCCTGACTCAC 10 0.25 No Hit CTCTGCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTCCTAAAG 10 0.25 No Hit CTTTCTTTTCCTCTGGCTACTAAGATGTTTCAGTTCGCCAGGTTGTCTCT 10 0.25 No Hit CTCCAGACTACAATTCGGACGGCACGGCCGCCCGATTCTCAAGCTGGGCT 9 0.22499999999999998 No Hit GGGGCATGATGACTTGGCCTCATCCTCTCCTTCCTCCGGCTTAACACCGG 9 0.22499999999999998 No Hit GACCTATTTGGGAATCTCCGGATCTATGCTTATTTTCAACTCCCCGAAGC 9 0.22499999999999998 No Hit CTAGCTTTCGTCTCTCAGTGTCAGTGTCGGCCCAGCAGAGTGCTTTCGCC 9 0.22499999999999998 No Hit CAGGGTTCCAAACTCATAGTGGCAACTAAACACGAGGGTTGCGCTCGTTG 9 0.22499999999999998 No Hit GGTCGTTCGAGCTTTTCCTGGGAGTATGGCATCGGTTACATACTTCAGTG 9 0.22499999999999998 No Hit CTCCTTTATCACTGAGCGGTCATTTAGGGGCCTTAGCTGGTGATCCGGGC 8 0.2 No Hit CCCACCTGTGTCGGTTTCGGGTACAGGTACCCTTTTGTTGAAGGTCGTTC 8 0.2 No Hit CCTAGAGTAACTTTTATCCGTTGAGCGACGGCCCTTCCACTCGGCACCGT 8 0.2 No Hit CCACAGCTTCGGCAGATCGCTTAGCCCCGTTCATCTTCAGCGCAAGGGCG 8 0.2 No Hit GCCCCCGTCAATTCCTTTGAGTTTCATTCTTGCGAACGTACTCCCCAGGC 8 0.2 No Hit GTTCGTTAACGGAATTAACCAGACAAATCGCTCCACCAACTAAGAACGGC 8 0.2 No Hit GTTCAGGGTTCCAAACTCATAGTGGCAACTAAACACGAGGGTTGCGCTCG 8 0.2 No Hit CCTCCACGCGGCATTGCTCCGTCAGGCTTTCGCCCATTGCGGAAAATTCC 8 0.2 No Hit GCTTTCTTTTCCTCTGGCTACTAAGATGTTTCAGTTCGCCAGGTTGTCTC 8 0.2 No Hit GTCCTTAAACCTATAACCATCTTTCGGCTAACCTAGCCTCCTCCGTCCCT 8 0.2 No Hit CCACTGCTGCCTCCCGTAGGAGTCTGGGCCGTGTCTCAGTCCCAGTGTGG 7 0.17500000000000002 No Hit CTTCCCTCTAAGGCGGAACGCTCCCCTACCGATGCATTTTGACATCCCAC 7 0.17500000000000002 No Hit GGCGGTGTGTACAAAGGGCAGGGACGTAGTCAACGCGAGCTGATGACTCG 7 0.17500000000000002 No Hit GTACAAAGGGCAGGGACGTAGTCAACGCGAGCTGATGACTCGCGCTTACT 7 0.17500000000000002 No Hit GGCGATAATAAATACAGAAGTTGCGGTCAATAAGGTAGGGATCATCAAAA 7 0.17500000000000002 No Hit CTCCTACTCATCGGGGCATGGCGCTCGCCCAGATGGCCGGGTGTGGGTCG 7 0.17500000000000002 No Hit CCTCGCGGTACTTGTTCGCTATCGGTCTCTCGCCTGTATTTAGCCTTGGA 7 0.17500000000000002 No Hit CTCGCGGTACTTGTTCGCTATCGGTCTCTCGCCTGTATTTAGCCTTGGAC 7 0.17500000000000002 No Hit GTGTCCTTAAACCTATAACCATCTTTCGGCTAACCTAGCCTCCTCCGTCC 7 0.17500000000000002 No Hit CCGGCGATTACTAGCGATTCCTGCTTCATGCAGGCGAGTTGCAGCCTGCA 7 0.17500000000000002 No Hit GTACAAGGCCCGGGAACGGATTCACCGCCGTATGGCTGACCGGCGATTAC 7 0.17500000000000002 No Hit CCCCTACCGTACTCCAGCTTGGTAGTTTCCACCGCCTGTCCAGGGTTGAG 7 0.17500000000000002 No Hit CCCATCACGATGAAATTTCCCAAGATTACCCGGGCCTGTCGGCCAAGGCT 7 0.17500000000000002 No Hit CTTCGCTATCGGTCACCCAGGAGTATTTAGCCTTGCAAGGTGGTCCTTGC 7 0.17500000000000002 No Hit CCTAACCACAACTCATCCGCTGATTCTTCAACATCAGTCGGTTCGGACCT 7 0.17500000000000002 No Hit CCCAATCATTCCGGATAACGCTTGCATCCTCTGTCTTACCGCGGCTGCTG 6 0.15 No Hit GTGTGTACAAAGGGCAGGGACGTAGTCAACGCGAGCTGATGACTCGCGCT 6 0.15 No Hit CCAGGGAACAGTAAAGCTTCATAGGGTCTTTCTGTCCAGGTGCAGGTAGT 6 0.15 No Hit CCTGTATTTAGCCTTGGACGGAGTCTACCGCCCGATTTGGGCTGCATTCC 6 0.15 No Hit GTCGGGGCAGGCGGCGGGCGCAGGCGCCGCTTGCTAGCTTGGATTCTGAC 6 0.15 No Hit CTTGTCCGTACCAGTTCTGAGTCGACTGTTCAGCGCTCGGGGAAAGCCCC 6 0.15 No Hit CCAGTCAACTGCTGCGCCTCAACGCATTTCGGGGAGAACCAGCTAGCTCT 6 0.15 No Hit CCCGGAACCCAAAGACTTTGATTTCTCATAAGGTGCCGGCGGAGTCCTAT 6 0.15 No Hit GTCGCCCAGGGCATAAGGGGCATGATGACTTGGCCTCATCCTCTCCTTCC 6 0.15 No Hit CCTCGATTTGGTACCGCTCGCGCAGCCCGCACCGAAACAGTGCTTTACCC 6 0.15 No Hit ATCGTTTACGGCTAGGACTACTGGGGTCTCTAATCCCATTTGCTCCCCTA 6 0.15 No Hit CCACAATATTATAAGTCTCTTCCTCTTGACCAAATTTGTAACCCTCATTA 6 0.15 No Hit CCGGCTCATTCTTCAACAGGCACGCGGTCAGAGATCACTTTCCCCTCCCA 6 0.15 No Hit CTTGGGTTTTCGGGGCATTGGATTCTCACCAATGTTTTCGTTACTCAAGC 6 0.15 No Hit GTTCCGTTCCCTTAACCAAGCCACTGCCTATGAGTCGCCGGCTCATTCTT 6 0.15 No Hit ACCTGTTGTCCATCGACTACGCCTTTCGGCCTGATCTTAGGCCCTGACTC 6 0.15 No Hit CTAGTATTCAGAGTTTGCCTCGATTTGGTACCGCTCGCGCAGCCCGCACC 6 0.15 No Hit GCTCCTCAGCCTACGGGGTATTAGCAACCGTTTCCAGTTGTTGTTCCCCT 6 0.15 No Hit GCTCCCTTCTGCCTTTGCACTCGAGGACCAATGTCCGTCTGGCCCGAGGA 6 0.15 No Hit GCTCATTCTTCAACAGGCACGCGGTCAGAGATCACTTTCCCCTCCCACTG 6 0.15 No Hit CACCTGTGTCGGTTTCGGGTACAGGTACCCTTTTGTTGAAGGTCGTTCGA 5 0.125 No Hit GTTCCATTGGCCAGAGGCTGTTCACCTTGGAGACCTGATGCGGTTATGAG 5 0.125 No Hit CTCGCCTGTATTTAGCCTTGGACGGAGTCTACCGCCCGATTTGGGCTGCA 5 0.125 No Hit CTTCCTCTTGACCAAATTTGTAACCCTCATTAGCAGATTCATTTTCAGTA 5 0.125 No Hit GGCATAAGGGGCATGATGACTTGGCCTCATCCTCTCCTTCCTCCGGCTTA 5 0.125 No Hit CCTTAAACCTATAACCATCTTTCGGCTAACCTAGCCTCCTCCGTCCCTCC 5 0.125 No Hit CCTCACGGTACTACTTCGCTATCGGTCACCCAGGAGTATTTAGCCTTGCA 5 0.125 No Hit GTGGTTTCGCTGGATAGTAGACAGGGACAGTGGGAATCTCGTTAATCCAT 5 0.125 No Hit GTGAGCATTACGTTCGTGCATTACTTCCATACCAAGATTAGCACGGTTGA 5 0.125 No Hit CGGGAACGGATTCACCGCCGTATGGCTGACCGGCGATTACTAGCGATTCC 5 0.125 No Hit GCCCCATAGAAACTGTCTACCTGAGACTGTCCCTTGGCCCGCGGGTCTGA 5 0.125 No Hit GTCCATCGACTACGCCTTTCGGCCTGATCTTAGGCCCTGACTCACCCTCC 5 0.125 No Hit CCACATGCTCCACCGCTTGTGCGGGCCCCCGTCAATTCCTTTGAGTTTCA 5 0.125 No Hit CGTTCATCTTCAGCGCAAGGGCGCTCGATCAGTGAGCTATTACGCACTCT 5 0.125 No Hit GCTCCCCTAGCTTTCGTCTCTCAGTGTCAGTGTCGGCCCAGCAGAGTGCT 5 0.125 No Hit GTTCGCTATCGGTCTCTCGCCTGTATTTAGCCTTGGACGGAGTCTACCGC 5 0.125 No Hit CTTCCACCTAAGCTGCGCAGGAAAGGCCCAAAGCCAATCCCAGGGAACAG 5 0.125 No Hit GTTGAGACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTC 5 0.125 No Hit CCCTAGCTTTCGTCTCTCAGTGTCAGTGTCGGCCCAGCAGAGTGCTTTCG 5 0.125 No Hit CCCCGTCAATTCCTTTGAGTTTCATTCTTGCGAACGTACTCCCCAGGCGG 5 0.125 No Hit CCGGAACCCAAAGACTTTGATTTCTCATAAGGTGCCGGCGGAGTCCTATA 5 0.125 No Hit CCCAGTGTGGCTGATCATCCTCTCGGACCAGCTACTGATCATCGCCTTGG 5 0.125 No Hit GCTCTGCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTCCTAAA 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0125 0.0 0.0 0.0 0.0 46-47 0.025 0.0 0.0 0.0 0.0 48-49 0.05 0.0 0.0 0.0 0.0 50-51 0.05 0.0 0.0 0.0 0.0 52-53 0.05 0.0 0.0 0.0 0.0 54-55 0.075 0.0 0.0 0.0 0.0 56-57 0.1 0.0 0.0 0.0 0.0 58-59 0.175 0.0 0.0 0.0 0.0 60-61 0.225 0.0 0.0 0.0 0.0 62-63 0.3125 0.0 0.0 0.0 0.0 64-65 0.375 0.0 0.0 0.0 0.0 66-67 0.4625 0.0 0.0 0.0 0.0 68-69 0.5625 0.0 0.0 0.0 0.0 70-71 0.65 0.0 0.0 0.0 0.0 72-73 0.8875 0.0 0.0 0.0 0.0 74-75 1.0 0.0 0.0 0.0 0.0 76-77 1.225 0.0 0.0 0.0 0.0 78-79 1.5750000000000002 0.0 0.0 0.0 0.0 80-81 1.7000000000000002 0.0 0.0 0.0 0.0 82-83 1.975 0.0 0.0 0.0 0.0 84-85 2.325 0.0 0.0 0.0 0.0 86-87 2.8125 0.0 0.0 0.0 0.0 88-89 3.325 0.0 0.0 0.0 0.0 90-91 4.0 0.0 0.0 0.0 0.0 92-93 4.7 0.0 0.0 0.0 0.0 94-95 5.475 0.0 0.0 0.0 0.0 96-97 6.3375 0.0 0.0 0.0 0.0 98-99 7.15 0.0 0.0 0.0 0.0 100-101 8.0125 0.0 0.0 0.0 0.0 102-103 8.7875 0.0 0.0 0.0 0.0 104-105 9.5625 0.0 0.0 0.0 0.0 106-107 10.524999999999999 0.0 0.0 0.0 0.0 108-109 11.0875 0.0 0.0 0.0 0.0 110-111 12.0125 0.0 0.0 0.0 0.0 112-113 13.0125 0.0 0.0 0.0 0.0 114-115 13.9375 0.0 0.0 0.0 0.0 116-117 14.925 0.0 0.0 0.0 0.0 118-119 15.787500000000001 0.0 0.0 0.0 0.0 120-121 16.7125 0.0 0.0 0.0 0.0 122-123 17.375 0.0 0.0 0.0 0.0 124-125 18.4375 0.0 0.0 0.0 0.0 126-127 19.425 0.0 0.0 0.0 0.0 128-129 20.3875 0.0 0.0 0.0 0.0 130-131 21.362499999999997 0.0 0.0 0.0 0.0 132-133 22.225 0.0 0.0 0.0 0.0 134-135 22.975 0.0 0.0 0.0 0.0 136-137 23.8125 0.0 0.0 0.0 0.0 138-139 24.7125 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CATGATG 10 0.006585701 146.75949 3 GGCATGA 10 0.006585701 146.75949 1 ATGATGA 10 0.006585701 146.75949 4 GCATGAT 10 0.006585701 146.75949 2 GCTCTGC 10 0.006585701 146.75949 3 GCATTTT 10 0.006585701 146.75949 145 GTGCTCT 10 0.006585701 146.75949 1 TGACTTG 10 0.006841402 144.925 8 ATGACTT 10 0.006841402 144.925 7 GACTTGG 10 0.006841402 144.925 9 CTCCTTT 40 0.0053541646 55.03481 1 TAAATCA 20 0.005950134 28.985 85-89 >>END_MODULE SRR6941555 read2 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR6941555_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 52 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 34.08075 35.0 35.0 35.0 32.0 35.0 2 34.413 35.0 35.0 35.0 33.0 35.0 3 34.423 35.0 35.0 35.0 33.0 35.0 4 34.32225 35.0 35.0 35.0 33.0 35.0 5 34.39825 35.0 35.0 35.0 33.0 35.0 6 39.0985 40.0 40.0 40.0 38.0 40.0 7 39.15625 40.0 40.0 40.0 38.0 40.0 8 39.22475 40.0 40.0 40.0 39.0 40.0 9 39.26175 40.0 40.0 40.0 39.0 40.0 10-14 39.228300000000004 40.0 40.0 40.0 39.0 40.0 15-19 39.25385 40.0 40.0 40.0 39.0 40.0 20-24 39.202549999999995 40.0 40.0 40.0 38.8 40.0 25-29 39.18535 40.0 40.0 40.0 39.0 40.0 30-34 39.1371 40.0 40.0 40.0 38.4 40.0 35-39 39.182750000000006 40.0 40.0 40.0 38.8 40.0 40-44 39.051050000000004 40.0 39.8 40.0 38.2 40.0 45-49 38.87135 40.0 39.2 40.0 37.2 40.0 50-54 38.889149999999994 40.0 39.2 40.0 37.4 40.0 55-59 38.968399999999995 40.0 39.2 40.0 38.0 40.0 60-64 38.96435 40.0 39.2 40.0 37.8 40.0 65-69 38.74675 40.0 39.0 40.0 36.6 40.0 70-74 38.748400000000004 40.0 39.0 40.0 36.6 40.0 75-79 38.5901 40.0 39.0 40.0 36.2 40.0 80-84 38.6823 40.0 39.0 40.0 36.4 40.0 85-89 38.58565 40.0 39.0 40.0 36.2 40.0 90-94 38.5432 40.0 39.0 40.0 36.0 40.0 95-99 38.4326 40.0 39.0 40.0 36.0 40.0 100-104 37.4763 39.0 38.0 39.6 33.0 39.8 105-109 38.119099999999996 40.0 39.0 40.0 35.0 40.0 110-114 34.664 36.4 34.2 38.4 29.2 38.6 115-119 18.58755 16.6 15.6 23.4 12.8 30.0 120-124 2.0 2.0 2.0 2.0 2.0 2.0 125-129 2.0 2.0 2.0 2.0 2.0 2.0 130-134 2.0 2.0 2.0 2.0 2.0 2.0 135-139 2.0 2.0 2.0 2.0 2.0 2.0 140-144 2.0 2.0 2.0 2.0 2.0 2.0 145-149 2.0 2.0 2.0 2.0 2.0 2.0 150-151 2.0 2.0 2.0 2.0 2.0 2.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 1.0 8 1.0 9 2.0 10 1.0 11 0.0 12 0.0 13 0.0 14 1.0 15 2.0 16 1.0 17 1.0 18 6.0 19 10.0 20 8.0 21 14.0 22 27.0 23 36.0 24 48.0 25 71.0 26 74.0 27 113.0 28 161.0 29 316.0 30 2038.0 31 1068.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.21331997996995 15.948923385077615 6.609914872308463 35.22784176264396 2 32.79959969977483 22.141606204653492 25.8443832874656 19.21441080810608 3 22.86715036277208 23.692769577182887 29.92244183137353 23.517638228671505 4 28.57142857142857 29.797348011008257 21.641230923192396 19.989992494370778 5 33.049787340505375 30.69802351763823 17.212909682261696 19.039279459594695 6 26.474999999999998 35.475 16.725 21.325 7 23.125 22.8 30.825000000000003 23.25 8 25.55 23.575 23.875 27.0 9 29.175 22.0 24.349999999999998 24.474999999999998 10-14 29.23 25.619999999999997 21.240000000000002 23.91 15-19 29.9 24.86 22.015 23.225 20-24 29.515 25.135 22.085 23.265 25-29 29.725 26.08 21.355 22.84 30-34 29.025000000000002 26.06 21.735 23.18 35-39 29.371468573428672 27.1863593179659 21.14605730286514 22.296114805740284 40-44 29.952995299529956 26.67766776677668 21.532153215321532 21.837183718371836 45-49 29.245094112935522 26.927312775330396 21.125350420504603 22.702242691229475 50-54 30.505252626313155 25.64782391195598 21.555777888944473 22.291145572786395 55-59 28.928803722419573 26.17701505978886 22.014309301045678 22.879871916745884 60-64 29.591836734693878 25.310124049619848 22.27891156462585 22.819127651060427 65-69 30.417166866746697 25.850340136054424 21.303521408563427 22.428971588635456 70-74 30.370629720402142 25.188816085629973 22.052718451458013 22.38783574250988 75-79 30.36455468320248 24.69870480572086 21.86327949192379 23.073461019152873 80-84 29.323195437947074 26.051723275473964 22.47011155019759 22.15496973638137 85-89 29.87149357467873 25.5812790639532 21.086054302715134 23.461173058652932 90-94 29.87 25.905 21.93 22.295 95-99 30.675 25.564999999999998 21.095 22.665 100-104 29.53647682384119 27.35636781839092 21.241062053102656 21.86609330466523 105-109 30.855 24.725 22.62 21.8 110-114 30.882868446091443 25.935199559316942 21.643547498622866 21.538384495968753 115-119 30.524532563684275 26.364663069393902 21.872254988078804 21.238549378843015 120-124 27.738336713995942 41.34127789046653 13.38108519269777 17.539300202839755 125-129 NaN NaN NaN NaN 130-134 31.082946417367296 25.98820429163007 20.127995984439703 22.80085330656293 135-139 30.334035510081254 25.599358009830475 21.341157588524425 22.72544889156385 140-144 31.81331485797065 25.111796938968318 21.81142533224161 21.263462870819424 145-149 33.01650825412706 25.512756378189096 20.31015507753877 21.160580290145074 150-151 33.04957436154231 25.225338007010517 19.379068602904358 22.346019028542813 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.5 11 0.5 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 1.0 25 1.5 26 1.5 27 2.0 28 3.5 29 4.5 30 4.0 31 6.5 32 7.5 33 13.0 34 17.0 35 20.0 36 25.5 37 32.5 38 46.0 39 61.0 40 62.0 41 61.0 42 65.5 43 69.0 44 75.0 45 84.0 46 91.0 47 87.5 48 86.0 49 96.0 50 130.0 51 163.0 52 167.5 53 211.0 54 329.0 55 390.0 56 341.0 57 254.0 58 205.0 59 185.0 60 145.5 61 115.0 62 95.5 63 60.5 64 27.5 65 14.5 66 11.5 67 16.5 68 24.0 69 22.0 70 16.0 71 14.0 72 9.0 73 3.0 74 3.0 75 6.5 76 5.0 77 3.0 78 4.5 79 3.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 0.15 2 0.075 3 0.075 4 0.075 5 0.075 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.005 40-44 0.01 45-49 0.12 50-54 0.05 55-59 0.065 60-64 0.04 65-69 0.04 70-74 0.034999999999999996 75-79 0.015 80-84 0.045 85-89 0.005 90-94 0.0 95-99 0.0 100-104 0.005 105-109 0.0 110-114 0.155 115-119 20.31 120-124 21.12 125-129 100.0 130-134 60.155 135-139 0.31 140-144 20.615 145-149 80.01 150-151 0.15 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 64.25 #Duplication Level Percentage of deduplicated Percentage of total 1 74.78599221789884 48.05 2 14.24124513618677 18.3 3 5.330739299610895 10.274999999999999 4 2.4513618677042803 6.3 5 1.0116731517509727 3.25 6 0.622568093385214 2.4 7 0.4669260700389105 2.1 8 0.2723735408560311 1.4000000000000001 9 0.1556420233463035 0.8999999999999999 >10 0.6614785992217899 7.025 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTTCGATCCTGGCTCAGGATGAACGCTGGCGGCATGCTTAACACATGCAA 44 1.0999999999999999 No Hit CCTTACCAGGGCTTGACATGCCGCGAATCCTCTTGAAAGAGAGGGGTGCC 30 0.75 No Hit GGCTTGCGGTGGATACCTAGGCACCCAGAGACGAGGAAGGGCGTAGCAAG 24 0.6 No Hit CCTGCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGCAA 18 0.44999999999999996 No Hit CAACAACTGGAAACGGTTGCTAATACCCCGTAGGCTGAGGAGCAAAAGGA 16 0.4 No Hit CAGTGACCAGGCCCGGGCGACTGTTTACCAAAAACACAGGTCTCCGCAAA 15 0.375 No Hit GAACCTTACCAGGGCTTGACATGCCGCGAATCCTCTTGAAAGAGAGGGGT 14 0.35000000000000003 No Hit CTTACCAGGGCTTGACATGCCGCGAATCCTCTTGAAAGAGAGGGGTGCCC 14 0.35000000000000003 No Hit AGAACCTTACCAGGGCTTGACATGCCGCGAATCCTCTTGAAAGAGAGGGG 14 0.35000000000000003 No Hit GCCTGACGGAGCAATGCCGCGTGGAGGTGGAAGGCCTACGGGTCGTCAAC 13 0.325 No Hit CCTGGCTCAGGATGAACGCTGGCGGCATGCTTAACACATGCAAGTCGAAC 13 0.325 No Hit GGAAGGCCTACGGGTCGTCAACTTCTTTTCTCGGAGAAGAAACAATGACG 13 0.325 No Hit CCTAGGCACCCAGAGACGAGGAAGGGCGTAGCAAGCGACGAAATGCTTCG 12 0.3 No Hit GAACGCTGGCGGCATGCTTAACACATGCAAGTCGAACGGGAAGTGGTGTT 11 0.27499999999999997 No Hit GGATGAACGCTGGCGGCATGCTTAACACATGCAAGTCGAACGGGAAGTGG 10 0.25 No Hit CTTGCGGTGGATACCTAGGCACCCAGAGACGAGGAAGGGCGTAGCAAGCG 10 0.25 No Hit ATTATCTATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAAT 10 0.25 No Hit GTTGCTAATACCCCGTAGGCTGAGGAGCAAAAGGAGAAATCCGCCCAAGG 9 0.22499999999999998 No Hit GGGAAGCAACCGCGAAAGCGGGGGTCGACGAAGCGGAAGCGAGAATGTCG 9 0.22499999999999998 No Hit GGTGTTTCCAGTGGCGAACGGGTGAGTAACGCGTAAGAACCTGCCCTTGG 9 0.22499999999999998 No Hit AGTGACCAGGCCCGGGCGACTGTTTACCAAAAACACAGGTCTCCGCAAAG 9 0.22499999999999998 No Hit CTTGAAAGAGAGGGGTGCCCTCGGGAACGCGGACACAGGTGGTGCATGGC 8 0.2 No Hit GGAAAGAACACCAACGGCGAAAGCACTCTGCTGGGCCGACACTGACACTG 8 0.2 No Hit GGGAATTTCCGGTGGAGCGGTGAAATGCATTGAGATCGGAAAGAACACCA 8 0.2 No Hit GATGAACGCTGGCGGCATGCTTAACACATGCAAGTCGAACGGGAAGTGGT 8 0.2 No Hit CTTACCAGGTCCAGACATAGCAAGGATTGACAGACTGAGAGCTCTTTCTT 8 0.2 No Hit CGGGTGAGTAACGCGTAAGAACCTGCCCTTGGGAGGGGAACAACAACTGG 8 0.2 No Hit GAAACAATGACGGTATCTGAGGAATAAGCATCGGCTAACTCTGTGCCAGC 8 0.2 No Hit GGGAAACAGCCCGGATCACCAGCTAAGGCCCCTAAATGACCGCTCAGTGA 7 0.17500000000000002 No Hit GTTTGGCTGGGGCGGCACATCTGTTAAAAGATAACGCAGGTGTCCTAAGA 7 0.17500000000000002 No Hit CTGCTTTTTCAGGGTAAGAAGGGGTAGAGAAAATGCCTCGAGCCGAGGTC 7 0.17500000000000002 No Hit CTGCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGCAAG 7 0.17500000000000002 No Hit GTGAGATACCACTCTGGAAGAGCTCGGATTCTAACCTTGTGTCAGACCCG 7 0.17500000000000002 No Hit TGAACGCTGGCGGCATGCTTAACACATGCAAGTCGAACGGGAAGTGGTGT 7 0.17500000000000002 No Hit GGAGTCTGACATGCGTGCGAGTCGACGGGTTCTGAAACCTGGGATGCGCA 7 0.17500000000000002 No Hit GTTTCAACAACTCTCGTTCTTTACACTTCTTCTTGGCTGCTTGGCCTGTA 7 0.17500000000000002 No Hit GGTAGGGGCAGAGGGAATTTCCGGTGGAGCGGTGAAATGCATTGAGATCG 7 0.17500000000000002 No Hit GTTGGGTTAAGTCTCGCAACGAGCGCAACCCTCGTGTTTAGTTGCCACTA 7 0.17500000000000002 No Hit TGAAGAATGAGCCGGCGACTCATAGGCAGTGGCTTGGTTAAGGGAACGGA 7 0.17500000000000002 No Hit GGCTTTTCAAGTCCGCCGTCAAATCCCAGGGCTCAACCCTGGACAGGCGG 7 0.17500000000000002 No Hit GCTTCATCGTCGAGAGGGAAACAGCCCGGATCACCAGCTAAGGCCCCTAA 6 0.15 No Hit GTTGAAGAATGAGCCGGCGACTCATAGGCAGTGGCTTGGTTAAGGGAACG 6 0.15 No Hit GTCAGGCGGGACTACCCGCTGAGTTTAAGCATATAAATAAGCGGAGGAGA 6 0.15 No Hit CTGAGGAATAAGCATCGGCTAACTCTGTGCCAGCAGCCGCGGTAAGACAG 6 0.15 No Hit GCTTGCGGTGGATACCTAGGCACCCAGAGACGAGGAAGGGCGTAGCAAGC 6 0.15 No Hit GAGAAATCCGCCCAAGGAGGGGCTCGCGTCTGATTAGCTAGTTGGTGAGG 6 0.15 No Hit GGAACAACAACTGGAAACGGTTGCTAATACCCCGTAGGCTGAGGAGCAAA 6 0.15 No Hit GCTTGTTATATGGGTCGTGAGTGGGAACTTAGTTTCCGTTTGGGTATGCG 6 0.15 No Hit CTATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAA 6 0.15 No Hit CTGACACTGAGAGACGAAAGCTAGGGGAGCAAATGGGATTAGAGACCCCA 6 0.15 No Hit ATCCGCCCAAGGAGGGGCTCGCGTCTGATTAGCTAGTTGGTGAGGCAATA 6 0.15 No Hit GAGCAATACAAGCGTTGTGCTGCTAGGCGAAGCGGTTGAGTGCCGCACCC 6 0.15 No Hit GTGGAATCCCGTGTGAATCAGCAAGGACCACCTTGCAAGGCTAAATACTC 6 0.15 No Hit ACCTTACCAGGGCTTGACATGCCGCGAATCCTCTTGAAAGAGAGGGGTGC 6 0.15 No Hit CCCAAATAGGTCAACCTTTTAAACTGCCTGCTGAATCCATGAGCAGGCAA 6 0.15 No Hit CTTAGATGTTCTGGGCCGCACGCGCGCTACACTGATGTATTCAACGAGTA 6 0.15 No Hit CAGGGCTTGACATGCCGCGAATCCTCTTGAAAGAGAGGGGTGCCCTCGGG 5 0.125 No Hit CGAAAGATGGTTATAGGTTTAAGGACACAAGGTGACCCTGCTTTTTCAGG 5 0.125 No Hit GTCTGGTGCCAGCAGCCGCGGTAATTCCAGCTCCAATAGCGTATATTTAA 5 0.125 No Hit GGCTGATCTTCCCCAAGAGTCCACATCGACGGGAAGGTTTGGCACCTCGA 5 0.125 No Hit GGGTGAGTAACGCGTAAGAACCTGCCCTTGGGAGGGGAACAACAACTGGA 5 0.125 No Hit GGCTTGAGTAACGAAAACATTGGTGAGAATCCAATGCCCCGAAAACCCAA 5 0.125 No Hit GTTAAGTCTCGCAACGAGCGCAACCCTCGTGTTTAGTTGCCACTATGAGT 5 0.125 No Hit CTCAGGATGAACGCTGGCGGCATGCTTAACACATGCAAGTCGAACGGGAA 5 0.125 No Hit CCAAGATCTTACCATGACTGCAATTTTAGAGAGACGCGAAAGTACAAGCC 5 0.125 No Hit GTCGTCAACTTCTTTTCTCGGAGAAGAAACAATGACGGTATCTGAGGAAT 5 0.125 No Hit GGAGGGGCTCGCGTCTGATTAGCTAGTTGGTGAGGCAATAGCTTACCAAG 5 0.125 No Hit GGGAGCTTGACTGCAAGACTCACCCGTCGAGCAGAGACGAAAGTCGGCCT 5 0.125 No Hit AGCGGTGAAATGCATTGAGATCGGAAAGAACACCAACGGCGAAAGCACTC 5 0.125 No Hit GATAACATCATAGGATTCCGGTCCTATTGTGTTGGCCTTCGGGATCGGAG 5 0.125 No Hit GCATCGGCTAACTCTGTGCCAGCAGCCGCGGTAAGACAGAGGATGCAAGC 5 0.125 No Hit CAATAGCTTACCAAGGCGATGATCAGTAGCTGGTCCGAGAGGATGATCAG 5 0.125 No Hit CGAAGACGATCAGATACCGTCCTAGTCTCAACCATAAACGATGCCGACCA 5 0.125 No Hit AGGGAATTTCCGGTGGAGCGGTGAAATGCATTGAGATCGGAAAGAACACC 5 0.125 No Hit CCCAGGGCTCAACCCTGGACAGGCGGTGGAAACTACCAAGCTGGAGTACG 5 0.125 No Hit GTGGGAACTTAGTTTCCGTTTGGGTATGCGCCCTTGGATTGCTGTTGCAT 5 0.125 No Hit CGAAAGCTAGGGGAGCAAATGGGATTAGAGACCCCAGTAGTCCTAGCCGT 5 0.125 No Hit CTCGTGTTTAGTTGCCACTATGAGTTTGGAACCCTGAACAGACCGCCGGT 5 0.125 No Hit TGTCAAAATGCATCGGTAGGGGAGCGTTCCGCCTTAGAGGGAAGCAACCG 5 0.125 No Hit CCTGAACAGACCGCCGGTGTTAAGCCGGAGGAAGGAGAGGATGAGGCCAA 5 0.125 No Hit GGGAGAGCAATACAAGCGTTGTGCTGCTAGGCGAAGCGGTTGAGTGCCGC 5 0.125 No Hit GAGCAAATGGGATTAGAGACCCCAGTAGTCCTAGCCGTAAACGATGGATA 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0125 0.0 0.0 0.0 0.0 46-47 0.025 0.0 0.0 0.0 0.0 48-49 0.05 0.0 0.0 0.0 0.0 50-51 0.05 0.0 0.0 0.0 0.0 52-53 0.05 0.0 0.0 0.0 0.0 54-55 0.075 0.0 0.0 0.0 0.0 56-57 0.1 0.0 0.0 0.0 0.0 58-59 0.175 0.0 0.0 0.0 0.0 60-61 0.225 0.0 0.0 0.0 0.0 62-63 0.3125 0.0 0.0 0.0 0.0 64-65 0.375 0.0 0.0 0.0 0.0 66-67 0.4625 0.0 0.0 0.0 0.0 68-69 0.5625 0.0 0.0 0.0 0.0 70-71 0.65 0.0 0.0 0.0 0.0 72-73 0.8875 0.0 0.0 0.0 0.0 74-75 1.0 0.0 0.0 0.0 0.0 76-77 1.225 0.0 0.0 0.0 0.0 78-79 1.5750000000000002 0.0 0.0 0.0 0.0 80-81 1.725 0.0 0.0 0.0 0.0 82-83 2.025 0.0 0.0 0.0 0.0 84-85 2.375 0.0 0.0 0.0 0.0 86-87 2.8625 0.0 0.0 0.0 0.0 88-89 3.375 0.0 0.0 0.0 0.0 90-91 4.05 0.0 0.0 0.0 0.0 92-93 4.725 0.0 0.0 0.0 0.0 94-95 5.525 0.0 0.0 0.0 0.0 96-97 6.3875 0.0 0.0 0.0 0.0 98-99 7.2 0.0 0.0 0.0 0.0 100-101 8.0625 0.0 0.0 0.0 0.0 102-103 8.8625 0.0 0.0 0.0 0.0 104-105 9.625 0.0 0.0 0.0 0.0 106-107 10.55 0.0 0.0 0.0 0.0 108-109 10.725 0.0 0.0 0.0 0.0 110-111 10.725 0.0 0.0 0.0 0.0 112-113 10.725 0.0 0.0 0.0 0.0 114-115 10.725 0.0 0.0 0.0 0.0 116-117 10.725 0.0 0.0 0.0 0.0 118-119 10.725 0.0 0.0 0.0 0.0 120-121 10.725 0.0 0.0 0.0 0.0 122-123 10.725 0.0 0.0 0.0 0.0 124-125 10.725 0.0 0.0 0.0 0.0 126-127 10.725 0.0 0.0 0.0 0.0 128-129 10.725 0.0 0.0 0.0 0.0 130-131 10.725 0.0 0.0 0.0 0.0 132-133 10.725 0.0 0.0 0.0 0.0 134-135 10.725 0.0 0.0 0.0 0.0 136-137 10.725 0.0 0.0 0.0 0.0 138-139 10.725 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content pass >>END_MODULE Read 1391400 spots for SRR6941555.sra Written 1391400 spots for SRR6941555.sra Read 1391400 spots for SRR6941555.sra Written 1391400 spots for SRR6941555.sra Read 1391400 spots for SRR6941555.sra Written 1391400 spots for SRR6941555.sra Read 1391400 spots for SRR6941555.sra Written 1391400 spots for SRR6941555.sra Read 1391400 spots for SRR6941555.sra Written 1391400 spots for SRR6941555.sra Read 1391400 spots for SRR6941555.sra Written 1391400 spots for SRR6941555.sra Read 1391400 spots for SRR6941555.sra Written 1391400 spots for SRR6941555.sra Read 1391400 spots for SRR6941555.sra Written 1391400 spots for SRR6941555.sra Read 1391400 spots for SRR6941555.sra Written 1391400 spots for SRR6941555.sra Read 1391400 spots for SRR6941555.sra Written 1391400 spots for SRR6941555.sra Read 1391400 spots for SRR6941555.sra Written 1391400 spots for SRR6941555.sra Read 1391400 spots for SRR6941555.sra Written 1391400 spots for SRR6941555.sra Read 1391400 spots for SRR6941555.sra Written 1391400 spots for SRR6941555.sra Read 1391400 spots for SRR6941555.sra Written 1391400 spots for SRR6941555.sra Read 1391400 spots for SRR6941555.sra Written 1391400 spots for SRR6941555.sra Read 1391413 spots for SRR6941555.sra Written 1391413 spots for SRR6941555.sra Read 1391400 spots for SRR6941555.sra Written 1391400 spots for SRR6941555.sra Read 1391400 spots for SRR6941555.sra Written 1391400 spots for SRR6941555.sra Read 1391400 spots for SRR6941555.sra Written 1391400 spots for SRR6941555.sra Read 1391400 spots for SRR6941555.sra Written 1391400 spots for SRR6941555.sra SRR ids: ['SRR6941555.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_bwqnoagf SRR6941555.sra spots: 27828013 blocks: [[1, 1391400], [1391401, 2782800], [2782801, 4174200], [4174201, 5565600], [5565601, 6957000], [6957001, 8348400], [8348401, 9739800], [9739801, 11131200], [11131201, 12522600], [12522601, 13914000], [13914001, 15305400], [15305401, 16696800], [16696801, 18088200], [18088201, 19479600], [19479601, 20871000], [20871001, 22262400], [22262401, 23653800], [23653801, 25045200], [25045201, 26436600], [26436601, 27828013]] SRR6941555 file size 9408300 SRR6941555 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR6941555 SRR6941555_1.fastq SRR6941555_2.fastq Input file: SRR6941555_1.fastq Paired file: SRR6941555_2.fastq trimmed: SRR6941555-trimmed-pair1.fastq, SRR6941555-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Fri Dec 6 11:14:36 2024 >> started Fri Dec 6 11:15:19 2024 >> done (43.415s) 27828013 read pairs processed; of these: 25611 ( 0.09%) short read pairs filtered out after trimming by size control 21343 ( 0.08%) empty read pairs filtered out after trimming by size control 27781059 (99.83%) read pairs available; of these: 24087797 (86.71%) trimmed read pairs available after processing 3693262 (13.29%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 12 0.00% 19 15 0.00% 20 28 0.00% 21 29 0.00% 22 27 0.00% 23 32 0.00% 24 53 0.00% 25 48 0.00% 26 91 0.00% 27 84 0.00% 28 139 0.00% 29 165 0.00% 30 200 0.00% 31 258 0.00% 32 318 0.00% 33 361 0.00% 34 358 0.00% 35 447 0.00% 36 465 0.00% 37 500 0.00% 38 552 0.00% 39 699 0.00% 40 814 0.00% 41 924 0.00% 42 1011 0.00% 43 1050 0.00% 44 1150 0.00% 45 1170 0.00% 46 1380 0.00% 47 1499 0.01% 48 1658 0.01% 49 2052 0.01% 50 2121 0.01% 51 2469 0.01% 52 3043 0.01% 53 3371 0.01% 54 3748 0.01% 55 3845 0.01% 56 4264 0.02% 57 4783 0.02% 58 5746 0.02% 59 6319 0.02% 60 6835 0.02% 61 9225 0.03% 62 10676 0.04% 63 10504 0.04% 64 11725 0.04% 65 13299 0.05% 66 13907 0.05% 67 14845 0.05% 68 18688 0.07% 69 19748 0.07% 70 22430 0.08% 71 23528 0.08% 72 28946 0.10% 73 31307 0.11% 74 29671 0.11% 75 33850 0.12% 76 35529 0.13% 77 39395 0.14% 78 40981 0.15% 79 45560 0.16% 80 51222 0.18% 81 52980 0.19% 82 57693 0.21% 83 60020 0.22% 84 62378 0.22% 85 76222 0.27% 86 81679 0.29% 87 82654 0.30% 88 95900 0.35% 89 83998 0.30% 90 85453 0.31% 91 91990 0.33% 92 95403 0.34% 93 104651 0.38% 94 111238 0.40% 95 102837 0.37% 96 99090 0.36% 97 106489 0.38% 98 105170 0.38% 99 107142 0.39% 100 107879 0.39% 101 112598 0.41% 102 122739 0.44% 103 117923 0.42% 104 131231 0.47% 105 122206 0.44% 106 128096 0.46% 107 128712 0.46% 108 123684 0.45% 109 155770 0.56% 110 131705 0.47% 111 140895 0.51% 112 170535 0.61% 113 120438 0.43% 114 138328 0.50% 115 137631 0.50% 116 169641 0.61% 117 169204 0.61% 118 163535 0.59% 119 189045 0.68% 120 202651 0.73% 121 193824 0.70% 122 191398 0.69% 123 209705 0.75% 124 206745 0.74% 125 212903 0.77% 126 213372 0.77% 127 224179 0.81% 128 249584 0.90% 129 319439 1.15% 130 519534 1.87% 131 988798 3.56% 132 771882 2.78% 133 3612690 13.00% 134 4467862 16.08% 135 111519 0.40% 136 95583 0.34% 137 99308 0.36% 138 110187 0.40% 139 146970 0.53% 140 589200 2.12% 141 1398492 5.03% 142 260713 0.94% 143 1728790 6.22% 144 137903 0.50% 145 531247 1.91% 146 53322 0.19% 147 63503 0.23% 148 74971 0.27% 149 118536 0.43% 150 969036 3.49% 151 3693262 13.29% 27781059 reads passed initial QC criterion=sequence-density sequence-density=3.72 sequence-density-rank=1 fanout-score=1.91 fanout-score-rank=32 prefix-density=3.65 prefix-fanout=1.9 sequence=GTATTTAGCCTTG criterion=fanout-score sequence-density=0.01 sequence-density-rank=43 fanout-score=134.19 fanout-score-rank=1 prefix-density=0.68 prefix-fanout=1.0 sequence=TCGTTCGTTCGTTAGGATGCCTCAGCTGCATACATCACTGCACTTCCACTTGACACCTATTTAAACGGCTCGTCTCGCCGCTACCTTATCCTATTTCCATACTTCTGTCGCTCCATCCCCGTATGGGTGGAGAACCCGTCGCTGTCTCGGCTGTGATACCGGAGGCTCTAGGGAAGTCGGAGGAGAGAGCACTCATCTTGGGGTGGGCTTACTACTTATATGCTTTCAGCAGTTATCCTCTCCGCACTTGGCTACCCAGCGTTTACCGTAGGCACGATAACTGGTACACCAGAGGTGCGTCCTTCCCGGTCCTCTCGTACTAGGGAAAGGTCCTCTCAATGCTCTAACGCCCACACCGGATATGGACCGAACTGTCTCACGACGTTCTGAACCCAGCTCACGTACCGCATTAATGGGCGAACAGCCCAACCCTTGGAACCACCTACAGCTCCAGGTGGCGAAGAGCCGACATCGAGGTGCCAAACCTTCCCGTCGATGTGGACTCTTGGGG criterion=sequence-density sequence-density=1.04 sequence-density-rank=1 fanout-score=7.42 fanout-score-rank=11 prefix-density=5.29 prefix-fanout=1.5 sequence=TGGTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGTTAACGAACGAGACCTCAGCCTGCTAACTAGCTATGCGGAGCCATCCCTCCGCAGCTAGCTTCTTAGAGGGACTATCGCCGTTTAGGCGACGGAAGTTTGAGGCAATAACAGGTCTGTGATGCCCTTAGATGTTCTGGGCCGCACGCGCGCTACACTGATGTATTCAACGAGTATATAGCCTTGGCCGACAGGCCCGGGTAATCTTGGGAAATTTCATCGTGATGGGGATAGATCATTGCAATTGTTGGTCTTCAACGAGGAATGCCTAGTAAGCGCGAGTCATCAGCTCGCGTTGACTACGTCCCTGCCCTTTGTACACACC criterion=fanout-score sequence-density=0.02 sequence-density-rank=31 fanout-score=106.73 fanout-score-rank=1 prefix-density=2.48 prefix-fanout=1.0 sequence=CGCGGTAAGACGGGGGGGGCAAGTGTTCTTCGGAATGACTGGGCGTAAAGGGCACGTAGGCGGTGAATCGGGTTGAAAGTGAAAGTCGCCAAAAAGTGGCGGAATGCTCTCGAAACCAATTCACTTGAGTGAGACAGAGGAGAGTGGAATTTCGTGTGTAGGGGTGAAA Potential 3prime adapter identified. Now checking if in reference sequence /dee2/code/volunteer_pipeline.sh: line 905: -f: command not found /dee2/code/volunteer_pipeline.sh: line 906: -f: command not found Adapter seq not found in reference. Now shuffling file before clipping skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -t 20 -x GTATTTAGCCTTG -y TGGTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGTTAACGAACGAGACCTCAGCCTGCTAACTAGCTATGCGGAGCCATCCCTCCGCAGCTAGCTTCTTAGAGGGACTATCGCCGTTTAGGCGACGGAAGTTTGAGGCAATAACAGGTCTGTGATGCCCTTAGATGTTCTGGGCCGCACGCGCGCTACACTGATGTATTCAACGAGTATATAGCCTTGGCCGACAGGCCCGGGTAATCTTGGGAAATTTCATCGTGATGGGGATAGATCATTGCAATTGTTGGTCTTCAACGAGGAATGCCTAGTAAGCGCGAGTCATCAGCTCGCGTTGACTACGTCCCTGCCCTTTGTACACACC -o SRR6941555 SRR6941555_1.fastq SRR6941555_2.fastq Input file: SRR6941555_1.fastq Paired file: SRR6941555_2.fastq trimmed: SRR6941555-trimmed-pair1.fastq, SRR6941555-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): GTATTTAGCCTTG -- paired 3' end adapter sequence (-y): TGGTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGTTAACGAACGAGAC -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Fri Dec 6 11:18:38 2024 >> started Fri Dec 6 11:18:48 2024 >> done (9.890s) 9260353 read pairs processed; of these: 381 ( 0.00%) short read pairs filtered out after trimming by size control 629 ( 0.01%) empty read pairs filtered out after trimming by size control 9259343 (99.99%) read pairs available; of these: 14753 ( 0.16%) trimmed read pairs available after processing 9244590 (99.84%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 8 0.00% 19 7 0.00% 20 10 0.00% 21 11 0.00% 22 10 0.00% 23 11 0.00% 24 21 0.00% 25 18 0.00% 26 25 0.00% 27 27 0.00% 28 51 0.00% 29 52 0.00% 30 64 0.00% 31 95 0.00% 32 102 0.00% 33 110 0.00% 34 126 0.00% 35 145 0.00% 36 167 0.00% 37 150 0.00% 38 193 0.00% 39 243 0.00% 40 261 0.00% 41 299 0.00% 42 338 0.00% 43 377 0.00% 44 368 0.00% 45 397 0.00% 46 469 0.01% 47 515 0.01% 48 548 0.01% 49 682 0.01% 50 719 0.01% 51 839 0.01% 52 1025 0.01% 53 1117 0.01% 54 1233 0.01% 55 1257 0.01% 56 1434 0.02% 57 1520 0.02% 58 1897 0.02% 59 2126 0.02% 60 2329 0.03% 61 3127 0.03% 62 3518 0.04% 63 3481 0.04% 64 3860 0.04% 65 4430 0.05% 66 4600 0.05% 67 4969 0.05% 68 6140 0.07% 69 6627 0.07% 70 7454 0.08% 71 7848 0.08% 72 9646 0.10% 73 10455 0.11% 74 9763 0.11% 75 11125 0.12% 76 11831 0.13% 77 13294 0.14% 78 13824 0.15% 79 15121 0.16% 80 17055 0.18% 81 17492 0.19% 82 19229 0.21% 83 19784 0.21% 84 20804 0.22% 85 25250 0.27% 86 27237 0.29% 87 27394 0.30% 88 31768 0.34% 89 28190 0.30% 90 28583 0.31% 91 30400 0.33% 92 31811 0.34% 93 34922 0.38% 94 36817 0.40% 95 34220 0.37% 96 33293 0.36% 97 35531 0.38% 98 35193 0.38% 99 35940 0.39% 100 35939 0.39% 101 37470 0.40% 102 41011 0.44% 103 39292 0.42% 104 43775 0.47% 105 40897 0.44% 106 42658 0.46% 107 42799 0.46% 108 41023 0.44% 109 52080 0.56% 110 43705 0.47% 111 47174 0.51% 112 57143 0.62% 113 39332 0.42% 114 46375 0.50% 115 45752 0.49% 116 60177 0.65% 117 53368 0.58% 118 56998 0.62% 119 60046 0.65% 120 67320 0.73% 121 64534 0.70% 122 63925 0.69% 123 69936 0.76% 124 68846 0.74% 125 70839 0.77% 126 71359 0.77% 127 74881 0.81% 128 83571 0.90% 129 106986 1.16% 130 173067 1.87% 131 331590 3.58% 132 256255 2.77% 133 1203088 12.99% 134 1489425 16.09% 135 38482 0.42% 136 30727 0.33% 137 33063 0.36% 138 37001 0.40% 139 49322 0.53% 140 195309 2.11% 141 465819 5.03% 142 86686 0.94% 143 576125 6.22% 144 45885 0.50% 145 177977 1.92% 146 17668 0.19% 147 21189 0.23% 148 24973 0.27% 149 39336 0.42% 150 322016 3.48% 151 1231707 13.30% criterion=sequence-density sequence-density=3.71 sequence-density-rank=1 fanout-score=1.92 fanout-score-rank=32 prefix-density=3.65 prefix-fanout=1.9 sequence=GTATTTAGCCTTG criterion=fanout-score sequence-density=0.08 sequence-density-rank=20 fanout-score=67.56 fanout-score-rank=1 prefix-density=5.57 prefix-fanout=1.0 sequence=GCTGATCATCCGAAAAGACCAGCTAAGCATCATTGGCTTGGTCAGCCTTTACCTGACCAACTACCTAATACTACGCAGGCTCATCAAACAGCGCTTTTGAGCTTTCTTCAGGATTTGGCCCGAACTGTTCGGCAGATTCCCACGCGTTACGCACCCGTTCGC criterion=sequence-density sequence-density=1.03 sequence-density-rank=1 fanout-score=7.46 fanout-score-rank=14 prefix-density=5.28 prefix-fanout=1.5 sequence=TGGTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGTTAACGAACGAGACCTCAGCCTGCTAACTAGCTATGCGGAGCCATCCCTCCGCAGCTAGCTTCTTAGAGGGACTATCGCCGTTTAGGCGACGGAAGTTTGAGGCAATAACAGGTCTGTGATGCCCTTAGATGTTCTGGGCCGCACGCGCGCTACACTGATGTATTCAACGAGTATATAGCCTTGGCCGACAGGCCCGGGTAATCTTGGGAAATTTCATCGTGATGGGGATAGATCATTGCAATTGTTGGTCTTCAACGAGGAATGCCTAGTAAGCGCGAGTCATCAGCTCGCGTTGACTACGTCCCTGCCCTTTGTACACACC criterion=fanout-score sequence-density=0.05 sequence-density-rank=30 fanout-score=98.46 fanout-score-rank=1 prefix-density=4.50 prefix-fanout=1.0 sequence=GCGAACGGGTGCGTAACGCGTGGGAATCTGCCGAACAGTTCGGGCCAAATCCTGAAGAAAGCTCAAAAGCGCTGTTTGATGAGCCTGCGTAGTATTAGGTAGTTGGTCAGGTAAAGGCTGACCAAGCCAATGATGCTTAGCTGGTCTTTTCGGATGATCAGC SRR6941555 testing PE reads STAR mapping to Ensembl genome Started job on | Dec 06 11:19:45 Started mapping on | Dec 06 11:19:45 Finished on | Dec 06 11:21:37 Mapping speed, Million of reads per hour | 892.93 Number of input reads | 27780049 Average input read length | 262 UNIQUE READS: Uniquely mapped reads number | 9276714 Uniquely mapped reads % | 33.39% Average mapped length | 271.58 Number of splices: Total | 1034322 Number of splices: Annotated (sjdb) | 836973 Number of splices: GT/AG | 891047 Number of splices: GC/AG | 13136 Number of splices: AT/AC | 2691 Number of splices: Non-canonical | 127448 Mismatch rate per base, % | 0.28% Deletion rate per base | 0.00% Deletion average length | 1.51 Insertion rate per base | 0.00% Insertion average length | 1.59 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 12401744 % of reads mapped to multiple loci | 44.64% Number of reads mapped to too many loci | 1078598 % of reads mapped to too many loci | 3.88% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 2.52% % of reads unmapped: other | 15.56% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 6110988 6110988 6110988 N_multimapping 12401744 12401744 12401744 N_noFeature 6992730 9170198 7043498 N_ambiguous 128439 3066 72217 UnstrandedReadsAssigned:2155545 PositiveStrandReadsAssigned:103450 NegativeStrandReadsAssigned:2160999 Dataset is classified negative stranded MeadianReadLen=151 20thPercentileLength=126 echo kmer=121 SRR6941555 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,972 [index] number of k-mers: 66,720,672 [index] number of equivalence classes: 111,837 [quant] running in paired-end mode [quant] will process pair 1: SRR6941555-trimmed-pair1.fastq SRR6941555-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 27,780,049 reads, 6,558,863 reads pseudoaligned [quant] estimated average fragment length: 174.183 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 908 rounds 52973 SRR6941555.ke.tsv 35125 SRR6941555.se.tsv 88098 total ==> SRR6941555.ke.tsv <== target_id length eff_length est_counts tpm PNS24245 936 763.06 0 0 PNS24247 1044 870.817 2.22817 0.132529 PNS24249 1928 1754.82 11.5196 0.340011 PNS24246 1044 870.817 2.22817 0.132529 PNS24248 1044 870.817 2.22817 0.132529 PNS24244 1471 1297.82 0.795888 0.0317634 PNS24243 293 126.395 0 0 KQK14069 1603 1429.82 686.395 24.8646 KQK14071 474 301.688 51.4496 8.8331 ==> SRR6941555.se.tsv <== BRADI_1g14170v3 819 BRADI_1g53295v3 4 BRADI_1g59795v3 9 BRADI_1g07683v3 0 BRADI_1g00485v3 0 BRADI_1g20270v3 12 BRADI_1g74790v3 8 BRADI_1g09890v3 0 BRADI_1g77505v3 12 BRADI_1g48960v3 0 SRR6941555 completed mapping pipeline successfully