Starting /dee2/code/volunteer_pipeline.sh SRR6941556 current disk space = 1551469920256 free memory = 1607232364 SRR6941556 SRAfilesize eab504baa09ebad465c8dfa29ea8c885 SRR6941556.sra SRR6941556.sra file validated SRR6941556 is paired end SRR6941556 is conventional basespace SRR6941556 read1 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR6941556_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 34.76175 35.0 35.0 35.0 35.0 35.0 2 34.612 35.0 35.0 35.0 35.0 35.0 3 34.71825 35.0 35.0 35.0 35.0 35.0 4 34.711 35.0 35.0 35.0 35.0 35.0 5 34.638 35.0 35.0 35.0 35.0 35.0 6 39.5245 40.0 40.0 40.0 39.0 40.0 7 39.53225 40.0 40.0 40.0 39.0 40.0 8 39.527 40.0 40.0 40.0 39.0 40.0 9 39.50225 40.0 40.0 40.0 39.0 40.0 10-14 39.501200000000004 40.0 40.0 40.0 39.0 40.0 15-19 39.44180000000001 40.0 40.0 40.0 39.0 40.0 20-24 39.5002 40.0 40.0 40.0 39.0 40.0 25-29 39.47865 40.0 40.0 40.0 39.0 40.0 30-34 39.29795 40.0 40.0 40.0 39.0 40.0 35-39 39.414049999999996 40.0 40.0 40.0 39.0 40.0 40-44 39.42285 40.0 40.0 40.0 39.0 40.0 45-49 39.40970000000001 40.0 40.0 40.0 39.0 40.0 50-54 39.3474 40.0 40.0 40.0 39.0 40.0 55-59 39.32705 40.0 40.0 40.0 39.0 40.0 60-64 39.3532 40.0 40.0 40.0 39.0 40.0 65-69 39.3612 40.0 40.0 40.0 39.0 40.0 70-74 39.27524999999999 40.0 40.0 40.0 38.8 40.0 75-79 39.1315 40.0 40.0 40.0 38.6 40.0 80-84 39.19005 40.0 40.0 40.0 39.0 40.0 85-89 39.2397 40.0 40.0 40.0 39.0 40.0 90-94 39.1283 40.0 40.0 40.0 38.6 40.0 95-99 39.1316 40.0 40.0 40.0 38.4 40.0 100-104 38.285199999999996 39.2 38.6 39.4 36.8 39.8 105-109 39.1507 40.0 39.8 40.0 38.8 40.0 110-114 39.2068 40.0 40.0 40.0 38.8 40.0 115-119 39.2325 40.0 40.0 40.0 39.0 40.0 120-124 38.9782 40.0 39.8 40.0 37.8 40.0 125-129 38.92125 40.0 39.2 40.0 37.8 40.0 130-134 38.9048 40.0 39.0 40.0 37.8 40.0 135-139 38.83905 40.0 39.0 40.0 37.4 40.0 140-144 38.656600000000005 40.0 39.0 40.0 36.6 40.0 145-149 38.72695 40.0 39.0 40.0 37.6 40.0 150-151 36.994375 39.5 37.0 40.0 33.0 40.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 22 1.0 23 2.0 24 1.0 25 6.0 26 4.0 27 9.0 28 6.0 29 19.0 30 18.0 31 25.0 32 39.0 33 37.0 34 56.0 35 34.0 36 87.0 37 120.0 38 284.0 39 3252.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 59.11477869467367 10.202550637659416 4.576144036009002 26.106526631657918 2 23.65 11.799999999999999 30.95 33.6 3 23.775 18.95 26.125 31.15 4 25.874999999999996 26.700000000000003 22.2 25.224999999999998 5 24.555249310949637 33.750939614131795 22.95164119268354 18.74216988223503 6 19.950000000000003 37.775 21.825 20.45 7 14.725 31.1 37.7 16.475 8 16.875 26.950000000000003 31.2 24.975 9 17.599999999999998 24.224999999999998 34.55 23.625 10-14 20.14 32.84 24.795 22.225 15-19 20.9 30.44 24.91 23.75 20-24 19.475842752825848 31.189356807042113 26.597979393818143 22.736821046313892 25-29 22.14 30.36 26.505000000000003 20.995 30-34 22.15 31.905 24.09 21.855 35-39 22.035 29.525000000000002 26.245 22.195 40-44 19.919999999999998 30.8 25.900000000000002 23.380000000000003 45-49 20.075000000000003 29.220000000000002 27.785 22.919999999999998 50-54 21.12 29.985 25.759999999999998 23.135 55-59 20.72 29.595 25.590000000000003 24.095 60-64 19.875 29.32 26.919999999999998 23.885 65-69 19.66 30.869999999999997 26.035000000000004 23.435 70-74 20.31 30.115 24.265 25.31 75-79 21.01 29.544999999999998 26.595000000000002 22.85 80-84 22.955000000000002 28.994999999999997 25.319999999999997 22.73 85-89 21.98 29.099999999999998 25.540000000000003 23.380000000000003 90-94 19.06 30.235 26.47 24.235 95-99 19.91 31.365 24.2 24.525 100-104 20.73 31.185000000000002 24.349999999999998 23.735 105-109 20.419999999999998 30.669999999999998 25.845000000000002 23.064999999999998 110-114 21.16 28.660000000000004 25.169999999999998 25.009999999999998 115-119 20.52 30.985000000000003 23.26 25.235000000000003 120-124 21.250500600720866 29.88085702843412 23.368041649979975 25.500600720865034 125-129 20.44011002750688 30.402600650162544 24.996249062265566 24.16104026006502 130-134 21.865000000000002 30.9 22.785 24.45 135-139 22.405 30.69 24.099999999999998 22.805 140-144 22.650000000000002 29.415000000000003 25.22 22.715 145-149 20.880000000000003 30.95 24.315 23.855 150-151 21.049336338592536 31.592787377911346 22.664663160530928 24.693213122965187 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.5 2 0.5 3 0.0 4 0.0 5 0.0 6 0.5 7 0.5 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.5 18 1.0 19 1.5 20 2.0 21 2.0 22 2.5 23 1.5 24 0.5 25 3.5 26 8.5 27 12.0 28 13.5 29 17.0 30 21.0 31 23.0 32 25.0 33 28.0 34 34.0 35 49.0 36 91.0 37 186.0 38 239.0 39 250.0 40 280.0 41 299.0 42 243.0 43 208.0 44 208.5 45 186.0 46 165.0 47 157.0 48 144.0 49 96.5 50 81.5 51 77.0 52 70.0 53 61.0 54 77.5 55 101.5 56 102.0 57 76.0 58 60.5 59 66.5 60 53.5 61 32.5 62 22.5 63 21.0 64 22.5 65 23.0 66 15.0 67 6.5 68 4.5 69 5.5 70 5.0 71 4.0 72 3.5 73 2.0 74 0.5 75 0.5 76 1.0 77 1.0 78 1.0 79 0.5 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.025 2 0.0 3 0.0 4 0.0 5 0.22499999999999998 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.03 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.12 125-129 0.025 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.17500000000000002 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.224999999999994 #Duplication Level Percentage of deduplicated Percentage of total 1 84.25070309361189 52.425 2 7.8746484531940535 9.8 3 2.571313780634793 4.8 4 1.5267175572519083 3.8 5 0.6428284451586983 2.0 6 0.48212133386902367 1.7999999999999998 7 0.5624748895138609 2.45 8 0.28123744475693047 1.4000000000000001 9 0.24106066693451184 1.35 >10 1.5267175572519083 18.224999999999998 >50 0.040176777822418644 1.95 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GCGGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTACG 78 1.95 No Hit GGCCAAAATAACCATGAGCAGCCACAATATTATAAGTCTCTTCCTCTTGA 43 1.075 No Hit GGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTACGTT 42 1.05 No Hit GTGCTCTGCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTCCTA 36 0.8999999999999999 No Hit GCCAAAATAACCATGAGCAGCCACAATATTATAAGTCTCTTCCTCTTGAC 32 0.8 No Hit GCGACCTTGACTATCAACTACAGATTGGTTGAAATTGAATCCATTTAGGT 31 0.775 No Hit GCGGAAAAACCTTATCCATTAATAGCGGGAACTTCAAGAGCAGCTAGATC 30 0.75 No Hit CTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCATTAAT 29 0.7250000000000001 No Hit GCTCTGCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTCCTAAA 28 0.7000000000000001 No Hit GGATGCATAAGGATGTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAA 26 0.65 No Hit GCTTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCA 25 0.625 No Hit GTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTC 22 0.5499999999999999 No Hit GCCGAAAACACCAGCTACACCTAACATGTGAAATGGATGCATAAGGATGT 22 0.5499999999999999 No Hit CTTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCAT 22 0.5499999999999999 No Hit GCACGGTTGATGATATCAGCCCAAGTATTAATAACGCGACCTTGACTATC 18 0.44999999999999996 No Hit GTTGATGATATCAGCCCAAGTATTAATAACGCGACCTTGACTATCAACTA 18 0.44999999999999996 No Hit GCATAAGGATGTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAAAGTA 18 0.44999999999999996 No Hit GTTGAAACTAGCATATTGGAAGATTAATCGGCCAAAATAACCATGAGCAG 18 0.44999999999999996 No Hit GTGAGCATTACGTTCGTGCATTACTTCCATACCAAGATTAGCACGGTTGA 17 0.42500000000000004 No Hit GTTAGCGGAAAAACCTTATCCATTAATAGCGGGAACTTCAAGAGCAGCTA 17 0.42500000000000004 No Hit GGCGATAATAAATACAGAAGTTGCGGTCAATAAGGTAGGGATCATCAAAA 16 0.4 No Hit CGGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTACGT 15 0.375 No Hit GGAAAAACCTTATCCATTAATAGCGGGAACTTCAAGAGCAGCTAGATCTA 15 0.375 No Hit GCCAAGAAGAAGTGTAAAGAACGAGAGTTGTTGAAACTAGCATATTGGAA 14 0.35000000000000003 No Hit GTCGCAGCTGCAACAGGAGCTGAATATGCAACAGCAATCCAAGGGCGCAT 13 0.325 No Hit CCCAAGTATTAATAACGCGACCTTGACTATCAACTACAGATTGGTTGAAA 13 0.325 No Hit CATATAACAAGCTACACCAAGTAAGAAGTGTAGAACAATTAGCTCATAAG 13 0.325 No Hit GGTCAATAAGGTAGGGATCATCAAAACACCGAACCATCCGATGTAAAGAC 13 0.325 No Hit CTCTGCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTCCTAAAG 13 0.325 No Hit GGCGCATACCCAAACGGAAACTAAGTTCCCACTCACGACCCATATAACAA 12 0.3 No Hit GTTCGCTGGAGTAGGCTGCGAGGCCCAAGCGGCCAAGGAGTGCAGCGGCC 12 0.3 No Hit CTCATAAGGACCACCATTGTATAACCATTCATCAACGGATGCAGCTTCCC 12 0.3 No Hit GGCCAAGCAGCCAAGAAGAAGTGTAAAGAACGAGAGTTGTTGAAACTAGC 12 0.3 No Hit GATGATATCAGCCCAAGTATTAATAACGCGACCTTGACTATCAACTACAG 11 0.27499999999999997 No Hit GAGTTGTTGAAACTAGCATATTGGAAGATTAATCGGCCAAAATAACCATG 11 0.27499999999999997 No Hit GCGGTCAATAAGGTAGGGATCATCAAAACACCGAACCATCCGATGTAAAG 10 0.25 No Hit GATGCATAAGGATGTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAAA 10 0.25 No Hit GCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTCCTAAAGGCAT 10 0.25 No Hit GGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTACGTTC 10 0.25 No Hit GGAGGGGCTGCGATGAAGGCGATAATAAATACAGAAGTTGCGGTCAATAA 9 0.22499999999999998 No Hit GGTTGATGATATCAGCCCAAGTATTAATAACGCGACCTTGACTATCAACT 9 0.22499999999999998 No Hit GGGAAGTTGTGAGCATTACGTTCGTGCATTACTTCCATACCAAGATTAGC 9 0.22499999999999998 No Hit CCAGATTCCTACTACAGGCCAAGCAGCCAAGAAGAAGTGTAAAGAACGAG 9 0.22499999999999998 No Hit GCTGCGATGAAGGCGATAATAAATACAGAAGTTGCGGTCAATAAGGTAGG 9 0.22499999999999998 No Hit GCCCAAGTATTAATAACGCGACCTTGACTATCAACTACAGATTGGTTGAA 9 0.22499999999999998 No Hit CCCATATAACAAGCTACACCAAGTAAGAAGTGTAGAACAATTAGCTCATA 8 0.2 No Hit CAGTGAACCAGATTCCTACTACAGGCCAAGCAGCCAAGAAGAAGTGTAAA 8 0.2 No Hit CCATATAACAAGCTACACCAAGTAAGAAGTGTAGAACAATTAGCTCATAA 8 0.2 No Hit GCATTACGTTCGTGCATTACTTCCATACCAAGATTAGCACGGTTGATGAT 8 0.2 No Hit CCCTCATTAGCAGATTCATTTTCAGTAGTTTCCCTGATCAAACTAGAGGT 8 0.2 No Hit CGGAAAAACCTTATCCATTAATAGCGGGAACTTCAAGAGCAGCTAGATCT 8 0.2 No Hit CCTCATTAGCAGATTCATTTTCAGTAGTTTCCCTGATCAAACTAGAGGTT 8 0.2 No Hit GTCTCTTCCTCTTGACCAAATTTGTAACCCTCATTAGCAGATTCATTTTC 7 0.17500000000000002 No Hit CAAGTATTAATAACGCGACCTTGACTATCAACTACAGATTGGTTGAAATT 7 0.17500000000000002 No Hit GCCATAGTACTAATACCTAAAGCAGTGAACCAGATTCCTACTACAGGCCA 7 0.17500000000000002 No Hit TTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCATTA 7 0.17500000000000002 No Hit GCATATTGGAAGATTAATCGGCCAAAATAACCATGAGCAGCCACAATATT 7 0.17500000000000002 No Hit GCTAGTTATCCAGTTGCAGAAGCGACCCCACAGGCTTGTACTTTCGCGTC 7 0.17500000000000002 No Hit ACGCGACCTTGACTATCAACTACAGATTGGTTGAAATTGAATCCATTTAG 7 0.17500000000000002 No Hit GCTACACCTAACATGTGAAATGGATGCATAAGGATGTTGTGCTCTGCCTG 7 0.17500000000000002 No Hit GCTAGATCTAGAGGGAAGTTGTGAGCATTACGTTCGTGCATTACTTCCAT 7 0.17500000000000002 No Hit CTCTTGACCAAATTTGTAACCCTCATTAGCAGATTCATTTTCAGTAGTTT 7 0.17500000000000002 No Hit AGCAGCCACAATATTATAAGTCTCTTCCTCTTGACCAAATTTGTAACCCT 7 0.17500000000000002 No Hit GGAATAATAGCACCAGAGATAATATTGTTTCCATAAAGTAAAGAACCAGA 7 0.17500000000000002 No Hit GACCCATATAACAAGCTACACCAAGTAAGAAGTGTAGAACAATTAGCTCA 7 0.17500000000000002 No Hit GGTAAATCAAGAAAACAGCAGTCGCAGCTGCAACAGGAGCTGAATATGCA 7 0.17500000000000002 No Hit GTGCAATCCGATTGCCGCAGAAGTAGGAATAATAGCACCAGAGATAATAT 6 0.15 No Hit AGAGAAGCTTCCTTGACCAATAGGGTAAATCAAGAAAACAGCAGTCGCAG 6 0.15 No Hit AGGATGTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAAAGTACCAGA 6 0.15 No Hit CTGCGATGAAGGCGATAATAAATACAGAAGTTGCGGTCAATAAGGTAGGG 6 0.15 No Hit CGAGAGTTGTTGAAACTAGCATATTGGAAGATTAATCGGCCAAAATAACC 6 0.15 No Hit GGCATGATGACTTGGCCTCATCCTCTCCTTCCTCCGGCTTAACACCGGCG 6 0.15 No Hit GTGTACAAGGCCCGGGAACGGATTCACCGCCGTATGGCTGACCGGCGATT 6 0.15 No Hit CATTAATAGCGGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGA 6 0.15 No Hit CTCCACGCGGCATTGCTCCGTCAGGCTTTCGCCCATTGCGGAAAATTCCC 6 0.15 No Hit CCATGAGCAGCCACAATATTATAAGTCTCTTCCTCTTGACCAAATTTGTA 6 0.15 No Hit CGCGACCTTGACTATCAACTACAGATTGGTTGAAATTGAATCCATTTAGG 6 0.15 No Hit GTCAATAAGGTAGGGATCATCAAAACACCGAACCATCCGATGTAAAGACG 6 0.15 No Hit ACCAGCTACACCTAACATGTGAAATGGATGCATAAGGATGTTGTGCTCTG 5 0.125 No Hit GCGATAATAAATACAGAAGTTGCGGTCAATAAGGTAGGGATCATCAAAAC 5 0.125 No Hit CCGCAGAAGTAGGAATAATAGCACCAGAGATAATATTGTTTCCATAAAGT 5 0.125 No Hit GCGATGAAGGCGATAATAAATACAGAAGTTGCGGTCAATAAGGTAGGGAT 5 0.125 No Hit TTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCATT 5 0.125 No Hit CCTCACGGTACTACTTCGCTATCGGTCACCCAGGAGTATTTAGCCTTGCA 5 0.125 No Hit ATAAGGATGTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAAAGTACC 5 0.125 No Hit CCATTGTATAACCATTCATCAACGGATGCAGCTTCCCAAATTGGGTAAAA 5 0.125 No Hit GTAAAGAACGAGAGTTGTTGAAACTAGCATATTGGAAGATTAATCGGCCA 5 0.125 No Hit ATATAACAAGCTACACCAAGTAAGAAGTGTAGAACAATTAGCTCATAAGG 5 0.125 No Hit AGCGGAAAAACCTTATCCATTAATAGCGGGAACTTCAAGAGCAGCTAGAT 5 0.125 No Hit GGTCGTTCGAGCTTTTCCTGGGAGTATGGCATCGGTTACATACTTCAGTG 5 0.125 No Hit CATAAGGATGTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAAAGTAC 5 0.125 No Hit CATCAATATCTACTGGAGGGGCTGCGATGAAGGCGATAATAAATACAGAA 5 0.125 No Hit CTCATTAGCAGATTCATTTTCAGTAGTTTCCCTGATCAAACTAGAGGTTA 5 0.125 No Hit GGGGCTGCGATGAAGGCGATAATAAATACAGAAGTTGCGGTCAATAAGGT 5 0.125 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0125 0.0 0.0 0.0 0.0 60-61 0.025 0.0 0.0 0.0 0.0 62-63 0.025 0.0 0.0 0.0 0.0 64-65 0.025 0.0 0.0 0.0 0.0 66-67 0.025 0.0 0.0 0.0 0.0 68-69 0.037500000000000006 0.0 0.0 0.0 0.0 70-71 0.0625 0.0 0.0 0.0 0.0 72-73 0.0875 0.0 0.0 0.0 0.0 74-75 0.1125 0.0 0.0 0.0 0.0 76-77 0.15 0.0 0.0 0.0 0.0 78-79 0.1875 0.0 0.0 0.0 0.0 80-81 0.2625 0.0 0.0 0.0 0.0 82-83 0.375 0.0 0.0 0.0 0.0 84-85 0.4625 0.0 0.0 0.0 0.0 86-87 0.5874999999999999 0.0 0.0 0.0 0.0 88-89 0.8 0.0 0.0 0.0 0.0 90-91 0.925 0.0 0.0 0.0 0.0 92-93 1.025 0.0 0.0 0.0 0.0 94-95 1.1625 0.0 0.0 0.0 0.0 96-97 1.3875 0.0 0.0 0.0 0.0 98-99 1.6124999999999998 0.0 0.0 0.0 0.0 100-101 1.825 0.0 0.0 0.0 0.0 102-103 1.9625 0.0 0.0 0.0 0.0 104-105 2.175 0.0 0.0 0.0 0.0 106-107 2.3375 0.0 0.0 0.0 0.0 108-109 2.675 0.0 0.0 0.0 0.0 110-111 2.9625 0.0 0.0 0.0 0.0 112-113 3.2625 0.0 0.0 0.0 0.0 114-115 3.5875 0.0 0.0 0.0 0.0 116-117 3.9875 0.0 0.0 0.0 0.0 118-119 4.375 0.0 0.0 0.0 0.0 120-121 4.7125 0.0 0.0 0.0 0.0 122-123 5.175 0.0 0.0 0.0 0.0 124-125 5.7375 0.0 0.0 0.0 0.0 126-127 6.225 0.0 0.0 0.0 0.0 128-129 6.6875 0.0 0.0 0.0 0.0 130-131 7.125 0.0 0.0 0.0 0.0 132-133 7.5875 0.0 0.0 0.0 0.0 134-135 7.9375 0.0 0.0 0.0 0.0 136-137 8.3875 0.0 0.0 0.0 0.0 138-139 8.8375 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTGTACA 10 0.006832588 144.9875 7 GCGGTGT 10 0.006832588 144.9875 2 CGGTGTG 10 0.006832588 144.9875 3 CCCTCTA 10 0.006832588 144.9875 3 TGTACAA 10 0.006832588 144.9875 8 GGCGGTG 10 0.006832588 144.9875 1 GGTGTGT 10 0.006832588 144.9875 4 >>END_MODULE SRR6941556 read2 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR6941556_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 33.811 35.0 35.0 35.0 33.0 35.0 2 34.04175 35.0 35.0 35.0 33.0 35.0 3 34.335 35.0 35.0 35.0 33.0 35.0 4 34.2615 35.0 35.0 35.0 33.0 35.0 5 34.33325 35.0 35.0 35.0 33.0 35.0 6 39.05275 40.0 40.0 40.0 39.0 40.0 7 38.935 40.0 40.0 40.0 38.0 40.0 8 39.08325 40.0 40.0 40.0 39.0 40.0 9 39.17725 40.0 40.0 40.0 39.0 40.0 10-14 39.06985 40.0 40.0 40.0 38.8 40.0 15-19 39.1896 40.0 40.0 40.0 39.0 40.0 20-24 39.19815 40.0 40.0 40.0 39.0 40.0 25-29 39.174099999999996 40.0 40.0 40.0 39.0 40.0 30-34 39.12885 40.0 40.0 40.0 39.0 40.0 35-39 38.988 40.0 39.8 40.0 38.4 40.0 40-44 39.028 40.0 40.0 40.0 38.8 40.0 45-49 38.96905 40.0 40.0 40.0 38.6 40.0 50-54 38.8339 40.0 39.6 40.0 37.4 40.0 55-59 38.794999999999995 40.0 39.8 40.0 37.8 40.0 60-64 38.74975 40.0 39.2 40.0 37.2 40.0 65-69 38.741150000000005 40.0 39.4 40.0 37.2 40.0 70-74 38.6522 40.0 39.0 40.0 37.0 40.0 75-79 38.661649999999995 40.0 39.4 40.0 37.0 40.0 80-84 38.693650000000005 40.0 39.0 40.0 37.4 40.0 85-89 38.837599999999995 40.0 39.0 40.0 37.8 40.0 90-94 38.7186 40.0 39.0 40.0 37.2 40.0 95-99 38.495349999999995 40.0 39.0 40.0 36.0 40.0 100-104 37.2635 38.6 37.8 39.2 33.6 39.4 105-109 38.51375 40.0 39.0 40.0 36.4 40.0 110-114 38.624249999999996 40.0 39.0 40.0 37.0 40.0 115-119 38.4925 40.0 39.0 40.0 36.4 40.0 120-124 38.392399999999995 40.0 39.0 40.0 36.0 40.0 125-129 38.50795 40.0 39.0 40.0 36.2 40.0 130-134 38.34075 40.0 39.0 40.0 36.0 40.0 135-139 38.2967 40.0 39.0 40.0 36.0 40.0 140-144 37.89155 40.0 39.0 40.0 35.0 40.0 145-149 37.51135000000001 40.0 38.8 40.0 33.8 40.0 150-151 34.578125 38.0 34.5 39.5 24.5 40.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 0.0 4 2.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 1.0 12 2.0 13 2.0 14 0.0 15 0.0 16 3.0 17 1.0 18 2.0 19 2.0 20 7.0 21 6.0 22 5.0 23 13.0 24 7.0 25 15.0 26 12.0 27 16.0 28 15.0 29 24.0 30 24.0 31 28.0 32 37.0 33 38.0 34 60.0 35 76.0 36 96.0 37 173.0 38 365.0 39 2966.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 51.612084285351614 19.87814166031988 9.570957095709572 18.93881695861894 2 25.062845651080945 21.518350930115638 32.880844645550525 20.53795877325289 3 20.549999999999997 24.775 38.800000000000004 15.875 4 23.799999999999997 30.025000000000002 26.1 20.075000000000003 5 26.400000000000002 30.5 25.424999999999997 17.675 6 21.45 34.300000000000004 26.6 17.65 7 19.85 21.025 41.15 17.974999999999998 8 21.45 23.825 29.525000000000002 25.2 9 21.9 20.625 33.550000000000004 23.925 10-14 23.849999999999998 25.81 30.435000000000002 19.905 15-19 23.305 24.565 31.385 20.745 20-24 24.325 24.93 31.069999999999997 19.675 25-29 23.755000000000003 25.835 29.65 20.76 30-34 24.685000000000002 24.425 31.035 19.855 35-39 24.83 24.23 30.975 19.965 40-44 24.615000000000002 24.87 30.159999999999997 20.355 45-49 24.645 25.790000000000003 29.435 20.13 50-54 23.93 24.845 30.375000000000004 20.849999999999998 55-59 23.595 26.490000000000002 28.78 21.135 60-64 23.990000000000002 24.295 30.830000000000002 20.885 65-69 24.63 25.06 29.715000000000003 20.595 70-74 24.345 25.069999999999997 30.17 20.415 75-79 24.654999999999998 24.765 29.659999999999997 20.919999999999998 80-84 24.19 23.94 32.43 19.439999999999998 85-89 25.05 24.959999999999997 29.32 20.669999999999998 90-94 24.33 24.035 30.185000000000002 21.45 95-99 23.64 24.89 30.635 20.835 100-104 23.830000000000002 25.345000000000002 30.225 20.599999999999998 105-109 25.56 24.23 30.005 20.205000000000002 110-114 24.235 25.215 29.909999999999997 20.64 115-119 24.75 24.884999999999998 29.935000000000002 20.43 120-124 24.615000000000002 25.745 28.26 21.38 125-129 24.515 25.85 28.63 21.005 130-134 24.565 25.485000000000003 28.895 21.055 135-139 24.925 24.55 30.65 19.875 140-144 25.545 24.82 30.014999999999997 19.62 145-149 24.47 25.540000000000003 29.625 20.365 150-151 24.39543916802406 24.78386167146974 30.84826462849267 19.972434532013533 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.5 12 1.5 13 1.0 14 0.5 15 0.5 16 0.0 17 0.0 18 0.0 19 0.5 20 0.5 21 0.5 22 1.5 23 3.5 24 5.5 25 10.5 26 13.5 27 12.5 28 11.0 29 15.0 30 24.5 31 29.0 32 29.5 33 37.0 34 53.5 35 68.0 36 93.0 37 148.5 38 189.5 39 203.5 40 235.5 41 258.0 42 226.5 43 234.5 44 250.0 45 203.5 46 202.5 47 170.5 48 114.5 49 91.5 50 77.0 51 84.0 52 71.0 53 73.0 54 95.5 55 96.5 56 85.0 57 73.0 58 67.0 59 58.0 60 56.5 61 55.5 62 43.0 63 32.5 64 19.5 65 15.5 66 11.0 67 5.5 68 5.0 69 6.5 70 8.5 71 5.0 72 2.5 73 3.5 74 3.5 75 1.0 76 2.0 77 2.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 1.525 2 0.5499999999999999 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.2375 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 65.675 #Duplication Level Percentage of deduplicated Percentage of total 1 82.45146555005711 54.15 2 8.22230681385611 10.8 3 3.083365055196041 6.075 4 2.1317091739626948 5.6000000000000005 5 1.1419870574800153 3.75 6 0.9135896459840122 3.5999999999999996 7 0.41872858774267224 1.925 8 0.30452988199467074 1.6 9 0.26646364674533685 1.575 >10 1.0658545869813474 10.925 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GCACAACATCCTTATGCATCCATTTCACATGTTAGGTGTAGCTGGTGTTT 36 0.8999999999999999 No Hit GCTAGTTTCAACAACTCTCGTTCTTTACACTTCTTCTTGGCTGCTTGGCC 23 0.575 No Hit GCTTGTTATATGGGTCGTGAGTGGGAACTTAGTTTCCGTTTGGGTATGCG 23 0.575 No Hit ATTATCTATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAAT 23 0.575 No Hit GTTCGATCCTGGCTCAGGATGAACGCTGGCGGCATGCTTAACACATGCAA 21 0.525 No Hit GTTATTTTGGCCGATTAATCTTCCAATATGCTAGTTTCAACAACTCTCGT 19 0.475 No Hit TATCTATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTT 19 0.475 No Hit GTGGGAACTTAGTTTCCGTTTGGGTATGCGCCCTTGGATTGCTGTTGCAT 18 0.44999999999999996 No Hit AGGGAAACTACTGAAAATGAATCTGCTAATGAGGGTTACAAATTTGGTCA 18 0.44999999999999996 No Hit GTTCAACCTAAATGGATTCAATTTCAACCAATCTGTAGTTGATAGTCAAG 17 0.42500000000000004 No Hit ATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAA 16 0.4 No Hit GTTTCTGGTTCTTTACTTTATGGAAACAATATTATCTCTGGTGCTATTAT 16 0.4 No Hit GCTAATTGTTCTACACTTCTTACTTGGTGTAGCTTGTTATATGGGTCGTG 15 0.375 No Hit GCTGCGACTGCTGTTTTCTTGATTTACCCTATTGGTCAAGGAAGCTTCTC 15 0.375 No Hit GTAGGAATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCT 14 0.35000000000000003 No Hit GGAAGCTTCTCTGATGGTATGCCTTTAGGAATATCTGGTACTTTCAACTT 13 0.325 No Hit GCTGCATCCGTTGATGAATGGTTATACAATGGTGGTCCTTATGAGCTAAT 13 0.325 No Hit GTCAAGGTCGCGTTATTAATACTTGGGCTGATATCATCAACCGTGCTAAT 13 0.325 No Hit GGTACTTTCAACTTTATGATTGTATTCCAGGCAGAGCACAACATCCTTAT 12 0.3 No Hit GGGAAACTACTGAAAATGAATCTGCTAATGAGGGTTACAAATTTGGTCAA 11 0.27499999999999997 No Hit GTTGCATATTCAGCTCCTGTTGCAGCTGCGACTGCTGTTTTCTTGATTTA 11 0.27499999999999997 No Hit GCCTTTAGGAATATCTGGTACTTTCAACTTTATGATTGTATTCCAGGCAG 11 0.27499999999999997 No Hit GGTAACCTCTAGTTTGATCAGGGAAACTACTGAAAATGAATCTGCTAATG 10 0.25 No Hit ATCGGATTGCACTTTTACCCAATTTGGGAAGCTGCATCCGTTGATGAATG 10 0.25 No Hit ATTTGGTCAAGAGGAAGAGACTTATAATATTGTGGCTGCTCATGGTTATT 10 0.25 No Hit GTTATACAATGGTGGTCCTTATGAGCTAATTGTTCTACACTTCTTACTTG 10 0.25 No Hit GTTTGATCAGGGAAACTACTGAAAATGAATCTGCTAATGAGGGTTACAAA 10 0.25 No Hit ATCTATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTG 10 0.25 No Hit GGAAGAGACTTATAATATTGTGGCTGCTCATGGTTATTTTGGCCGATTAA 9 0.22499999999999998 No Hit GGTCGCTTCTGCAACTGGATAACTAGCACTGAAAATCGTCTTTACATCGG 9 0.22499999999999998 No Hit GGTGTTTTGATGATCCCTACCTTATTGACCGCAACTTCTGTATTTATTAT 9 0.22499999999999998 No Hit GAACGCTGGCGGCATGCTTAACACATGCAAGTCGAACGGGAAGTGGTGTT 9 0.22499999999999998 No Hit GTTTCAACAACTCTCGTTCTTTACACTTCTTCTTGGCTGCTTGGCCTGTA 9 0.22499999999999998 No Hit GTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGATCTTACCATGAC 9 0.22499999999999998 No Hit GAGCACAACATCCTTATGCATCCATTTCACATGTTAGGTGTAGCTGGTGT 9 0.22499999999999998 No Hit AGCACAACATCCTTATGCATCCATTTCACATGTTAGGTGTAGCTGGTGTT 8 0.2 No Hit CCTAAATGGATTCAATTTCAACCAATCTGTAGTTGATAGTCAAGGTCGCG 8 0.2 No Hit CTATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAA 8 0.2 No Hit GTAGCTTGTTATATGGGTCGTGAGTGGGAACTTAGTTTCCGTTTGGGTAT 8 0.2 No Hit GAAGAGACTTATAATATTGTGGCTGCTCATGGTTATTTTGGCCGATTAAT 8 0.2 No Hit TCTATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGA 8 0.2 No Hit GTACAAGCCTGTGGGGTCGCTTCTGCAACTGGATAACTAGCACTGAAAAT 8 0.2 No Hit CAACATCCTTATGCATCCATTTCACATGTTAGGTGTAGCTGGTGTTTTCG 8 0.2 No Hit GAAAATGAATCTGCTAATGAGGGTTACAAATTTGGTCAAGAGGAAGAGAC 7 0.17500000000000002 No Hit ATTTCAACCAATCTGTAGTTGATAGTCAAGGTCGCGTTATTAATACTTGG 7 0.17500000000000002 No Hit TATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAA 7 0.17500000000000002 No Hit GAGACTTATAATATTGTGGCTGCTCATGGTTATTTTGGCCGATTAATCTT 7 0.17500000000000002 No Hit CATGACTGCAATTTTAGAGAGACGCGAAAGTACAAGCCTGTGGGGTCGCT 7 0.17500000000000002 No Hit AATTGTTCTACACTTCTTACTTGGTGTAGCTTGTTATATGGGTCGTGAGT 7 0.17500000000000002 No Hit GTTTTGATGATCCCTACCTTATTGACCGCAACTTCTGTATTTATTATCGC 7 0.17500000000000002 No Hit GGCTTGCGGTGGATACCTAGGCACCCAGAGACGAGGAAGGGCGTAGCAAG 7 0.17500000000000002 No Hit GGGAACTTAGTTTCCGTTTGGGTATGCGCCCTTGGATTGCTGTTGCATAT 7 0.17500000000000002 No Hit GTTAGGTGTAGCTGGTGTTTTCGGCGGTTCCCTATTCAGTGCTATGCATG 7 0.17500000000000002 No Hit GTGTTTTCGGCGGTTCCCTATTCAGTGCTATGCATGGTTCCTTGGTAACC 7 0.17500000000000002 No Hit GTATGCGCCCTTGGATTGCTGTTGCATATTCAGCTCCTGTTGCAGCTGCG 6 0.15 No Hit GCTCATGGTTATTTTGGCCGATTAATCTTCCAATATGCTAGTTTCAACAA 6 0.15 No Hit GTTTTCTTGATTTACCCTATTGGTCAAGGAAGCTTCTCTGATGGTATGCC 6 0.15 No Hit GGAAACTACTGAAAATGAATCTGCTAATGAGGGTTACAAATTTGGTCAAG 6 0.15 No Hit GGTGTTTTCGGCGGTTCCCTATTCAGTGCTATGCATGGTTCCTTGGTAAC 6 0.15 No Hit CTTATGAGCTAATTGTTCTACACTTCTTACTTGGTGTAGCTTGTTATATG 6 0.15 No Hit GCAATTTTAGAGAGACGCGAAAGTACAAGCCTGTGGGGTCGCTTCTGCAA 6 0.15 No Hit GAGGGTTACAAATTTGGTCAAGAGGAAGAGACTTATAATATTGTGGCTGC 6 0.15 No Hit GGAATATCTGGTACTTTCAACTTTATGATTGTATTCCAGGCAGAGCACAA 6 0.15 No Hit AGTTGATAGTCAAGGTCGCGTTATTAATACTTGGGCTGATATCATCAACC 6 0.15 No Hit CTTTAGGAATATCTGGTACTTTCAACTTTATGATTGTATTCCAGGCAGAG 6 0.15 No Hit GATGAACGCTGGCGGCATGCTTAACACATGCAAGTCGAACGGGAAGTGGT 6 0.15 No Hit GTTACAAATTTGGTCAAGAGGAAGAGACTTATAATATTGTGGCTGCTCAT 6 0.15 No Hit CCTCTAGTTTGATCAGGGAAACTACTGAAAATGAATCTGCTAATGAGGGT 6 0.15 No Hit TAGCACTGAAAATCGTCTTTACATCGGATGGTTCGGTGTTTTGATGATCC 6 0.15 No Hit GGTTATACAATGGTGGTCCTTATGAGCTAATTGTTCTACACTTCTTACTT 6 0.15 No Hit GCATGGTTCCTTGGTAACCTCTAGTTTGATCAGGGAAACTACTGAAAATG 6 0.15 No Hit ATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATA 6 0.15 No Hit ATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCTAAATGG 6 0.15 No Hit GGAATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCTAAA 6 0.15 No Hit AGAGACTTATAATATTGTGGCTGCTCATGGTTATTTTGGCCGATTAATCT 6 0.15 No Hit CCCTATTCAGTGCTATGCATGGTTCCTTGGTAACCTCTAGTTTGATCAGG 6 0.15 No Hit ATACAATGGTGGTCCTTATGAGCTAATTGTTCTACACTTCTTACTTGGTG 6 0.15 No Hit GCTTTAGGTATTAGTACTATGGCGTTCAACCTAAATGGATTCAATTTCAA 6 0.15 No Hit CGGCGGTTCCCTATTCAGTGCTATGCATGGTTCCTTGGTAACCTCTAGTT 5 0.125 No Hit GTCGCTTCTGCAACTGGATAACTAGCACTGAAAATCGTCTTTACATCGGA 5 0.125 No Hit CTGGTACTTTCAACTTTATGATTGTATTCCAGGCAGAGCACAACATCCTT 5 0.125 No Hit GTCGTGAGTGGGAACTTAGTTTCCGTTTGGGTATGCGCCCTTGGATTGCT 5 0.125 No Hit ACTTAGTTTCCGTTTGGGTATGCGCCCTTGGATTGCTGTTGCATATTCAG 5 0.125 No Hit CCTGTAGTAGGAATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTT 5 0.125 No Hit CGTTGATGAATGGTTATACAATGGTGGTCCTTATGAGCTAATTGTTCTAC 5 0.125 No Hit AATCTTCCAATATGCTAGTTTCAACAACTCTCGTTCTTTACACTTCTTCT 5 0.125 No Hit AGCTAATTGTTCTACACTTCTTACTTGGTGTAGCTTGTTATATGGGTCGT 5 0.125 No Hit GTGCTATGCATGGTTCCTTGGTAACCTCTAGTTTGATCAGGGAAACTACT 5 0.125 No Hit ATTTCACATGTTAGGTGTAGCTGGTGTTTTCGGCGGTTCCCTATTCAGTG 5 0.125 No Hit CTTAGTTTCCGTTTGGGTATGCGCCCTTGGATTGCTGTTGCATATTCAGC 5 0.125 No Hit AGTCAAGGTCGCGTTATTAATACTTGGGCTGATATCATCAACCGTGCTAA 5 0.125 No Hit GGAAACAATATTATCTCTGGTGCTATTATTCCTACTTCTGCGGCAATCGG 5 0.125 No Hit ATATCTGGTACTTTCAACTTTATGATTGTATTCCAGGCAGAGCACAACAT 5 0.125 No Hit TATACAATGGTGGTCCTTATGAGCTAATTGTTCTACACTTCTTACTTGGT 5 0.125 No Hit ACTTGGTGTAGCTTGTTATATGGGTCGTGAGTGGGAACTTAGTTTCCGTT 5 0.125 No Hit GTTATTAATACTTGGGCTGATATCATCAACCGTGCTAATCTTGGTATGGA 5 0.125 No Hit GGATTGCACTTTTACCCAATTTGGGAAGCTGCATCCGTTGATGAATGGTT 5 0.125 No Hit CATCAACCGTGCTAATCTTGGTATGGAAGTAATGCACGAACGTAATGCTC 5 0.125 No Hit GTCCTTGCAATTTGAATAAACCAAGATCTTACCATGACTGCAATTTTAGA 5 0.125 No Hit GAGGAAGAGACTTATAATATTGTGGCTGCTCATGGTTATTTTGGCCGATT 5 0.125 No Hit CGAAAGTACAAGCCTGTGGGGTCGCTTCTGCAACTGGATAACTAGCACTG 5 0.125 No Hit CACATGTTAGGTGTAGCTGGTGTTTTCGGCGGTTCCCTATTCAGTGCTAT 5 0.125 No Hit GTATTTATTATCGCCTTCATCGCAGCCCCTCCAGTAGATATTGATGGTAT 5 0.125 No Hit CCTTACCAGGGCTTGACATGCCGCGAATCCTCTTGAAAGAGAGGGGTGCC 5 0.125 No Hit GAATGGTTATACAATGGTGGTCCTTATGAGCTAATTGTTCTACACTTCTT 5 0.125 No Hit GGGTCGTGAGTGGGAACTTAGTTTCCGTTTGGGTATGCGCCCTTGGATTG 5 0.125 No Hit CTGAAAATGAATCTGCTAATGAGGGTTACAAATTTGGTCAAGAGGAAGAG 5 0.125 No Hit GAATAAACCAAGATCTTACCATGACTGCAATTTTAGAGAGACGCGAAAGT 5 0.125 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0125 0.0 0.0 0.0 0.0 60-61 0.025 0.0 0.0 0.0 0.0 62-63 0.025 0.0 0.0 0.0 0.0 64-65 0.025 0.0 0.0 0.0 0.0 66-67 0.025 0.0 0.0 0.0 0.0 68-69 0.037500000000000006 0.0 0.0 0.0 0.0 70-71 0.0625 0.0 0.0 0.0 0.0 72-73 0.0875 0.0 0.0 0.0 0.0 74-75 0.1125 0.0 0.0 0.0 0.0 76-77 0.15 0.0 0.0 0.0 0.0 78-79 0.21250000000000002 0.0 0.0 0.0 0.0 80-81 0.2875 0.0 0.0 0.0 0.0 82-83 0.4 0.0 0.0 0.0 0.0 84-85 0.4875 0.0 0.0 0.0 0.0 86-87 0.6125 0.0 0.0 0.0 0.0 88-89 0.825 0.0 0.0 0.0 0.0 90-91 0.975 0.0 0.0 0.0 0.0 92-93 1.1124999999999998 0.0 0.0 0.0 0.0 94-95 1.2875 0.0 0.0 0.0 0.0 96-97 1.5125 0.0 0.0 0.0 0.0 98-99 1.7374999999999998 0.0 0.0 0.0 0.0 100-101 1.95 0.0 0.0 0.0 0.0 102-103 2.075 0.0 0.0 0.0 0.0 104-105 2.3 0.0 0.0 0.0 0.0 106-107 2.4625 0.0 0.0 0.0 0.0 108-109 2.7875 0.0 0.0 0.0 0.0 110-111 3.0625 0.0 0.0 0.0 0.0 112-113 3.3625 0.0 0.0 0.0 0.0 114-115 3.6875 0.0 0.0 0.0 0.0 116-117 4.075 0.0 0.0 0.0 0.0 118-119 4.4375 0.0 0.0 0.0 0.0 120-121 4.7875 0.0 0.0 0.0 0.0 122-123 5.25 0.0 0.0 0.0 0.0 124-125 5.8125 0.0 0.0 0.0 0.0 126-127 6.324999999999999 0.0 0.0 0.0 0.0 128-129 6.7875 0.0 0.0 0.0 0.0 130-131 7.25 0.0 0.0 0.0 0.0 132-133 7.7375 0.0 0.0 0.0 0.0 134-135 8.075 0.0 0.0 0.0 0.0 136-137 8.525 0.0 0.0 0.0 0.0 138-139 9.0375 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content pass >>END_MODULE Read 1701052 spots for SRR6941556.sra Written 1701052 spots for SRR6941556.sra Read 1701052 spots for SRR6941556.sra Written 1701052 spots for SRR6941556.sra Read 1701052 spots for SRR6941556.sra Written 1701052 spots for SRR6941556.sra Read 1701052 spots for SRR6941556.sra Written 1701052 spots for SRR6941556.sra Read 1701052 spots for SRR6941556.sra Written 1701052 spots for SRR6941556.sra Read 1701052 spots for SRR6941556.sra Written 1701052 spots for SRR6941556.sra Read 1701052 spots for SRR6941556.sra Written 1701052 spots for SRR6941556.sra Read 1701052 spots for SRR6941556.sra Written 1701052 spots for SRR6941556.sra Read 1701052 spots for SRR6941556.sra Written 1701052 spots for SRR6941556.sra Read 1701052 spots for SRR6941556.sra Written 1701052 spots for SRR6941556.sra Read 1701052 spots for SRR6941556.sra Written 1701052 spots for SRR6941556.sra Read 1701052 spots for SRR6941556.sra Written 1701052 spots for SRR6941556.sra Read 1701052 spots for SRR6941556.sra Written 1701052 spots for SRR6941556.sra Read 1701052 spots for SRR6941556.sra Written 1701052 spots for SRR6941556.sra Read 1701052 spots for SRR6941556.sra Written 1701052 spots for SRR6941556.sra Read 1701052 spots for SRR6941556.sra Written 1701052 spots for SRR6941556.sra Read 1701052 spots for SRR6941556.sra Written 1701052 spots for SRR6941556.sra Read 1701052 spots for SRR6941556.sra Written 1701052 spots for SRR6941556.sra Read 1701052 spots for SRR6941556.sra Written 1701052 spots for SRR6941556.sra Read 1701066 spots for SRR6941556.sra Written 1701066 spots for SRR6941556.sra SRR ids: ['SRR6941556.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_b8vcru9t SRR6941556.sra spots: 34021054 blocks: [[1, 1701052], [1701053, 3402104], [3402105, 5103156], [5103157, 6804208], [6804209, 8505260], [8505261, 10206312], [10206313, 11907364], [11907365, 13608416], [13608417, 15309468], [15309469, 17010520], [17010521, 18711572], [18711573, 20412624], [20412625, 22113676], [22113677, 23814728], [23814729, 25515780], [25515781, 27216832], [27216833, 28917884], [28917885, 30618936], [30618937, 32319988], [32319989, 34021054]] SRR6941556 file size 11506918 SRR6941556 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR6941556 SRR6941556_1.fastq SRR6941556_2.fastq Input file: SRR6941556_1.fastq Paired file: SRR6941556_2.fastq trimmed: SRR6941556-trimmed-pair1.fastq, SRR6941556-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Fri Dec 6 11:15:37 2024 >> started Fri Dec 6 11:16:29 2024 >> done (51.816s) 34021054 read pairs processed; of these: 19371 ( 0.06%) short read pairs filtered out after trimming by size control 16773 ( 0.05%) empty read pairs filtered out after trimming by size control 33984910 (99.89%) read pairs available; of these: 7123803 (20.96%) trimmed read pairs available after processing 26861107 (79.04%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 2 0.00% 19 3 0.00% 20 2 0.00% 21 10 0.00% 22 4 0.00% 23 14 0.00% 24 11 0.00% 25 6 0.00% 26 13 0.00% 27 21 0.00% 28 12 0.00% 29 17 0.00% 30 21 0.00% 31 30 0.00% 32 27 0.00% 33 22 0.00% 34 25 0.00% 35 41 0.00% 36 40 0.00% 37 38 0.00% 38 79 0.00% 39 92 0.00% 40 106 0.00% 41 139 0.00% 42 131 0.00% 43 150 0.00% 44 164 0.00% 45 195 0.00% 46 220 0.00% 47 254 0.00% 48 266 0.00% 49 348 0.00% 50 389 0.00% 51 505 0.00% 52 600 0.00% 53 622 0.00% 54 696 0.00% 55 837 0.00% 56 928 0.00% 57 1049 0.00% 58 1214 0.00% 59 1279 0.00% 60 1488 0.00% 61 1758 0.01% 62 2195 0.01% 63 2467 0.01% 64 2877 0.01% 65 3119 0.01% 66 3434 0.01% 67 3502 0.01% 68 4270 0.01% 69 5139 0.02% 70 5748 0.02% 71 6611 0.02% 72 7856 0.02% 73 8612 0.03% 74 8488 0.02% 75 9726 0.03% 76 9908 0.03% 77 11456 0.03% 78 11425 0.03% 79 12491 0.04% 80 14154 0.04% 81 15492 0.05% 82 17546 0.05% 83 18064 0.05% 84 19617 0.06% 85 23406 0.07% 86 24638 0.07% 87 25891 0.08% 88 28593 0.08% 89 28885 0.08% 90 32864 0.10% 91 32739 0.10% 92 37841 0.11% 93 38463 0.11% 94 40758 0.12% 95 43328 0.13% 96 41769 0.12% 97 41330 0.12% 98 40500 0.12% 99 42761 0.13% 100 44290 0.13% 101 48122 0.14% 102 51766 0.15% 103 52039 0.15% 104 53880 0.16% 105 56147 0.17% 106 54222 0.16% 107 55473 0.16% 108 57619 0.17% 109 61591 0.18% 110 62587 0.18% 111 68769 0.20% 112 70090 0.21% 113 67675 0.20% 114 73713 0.22% 115 67233 0.20% 116 68470 0.20% 117 65774 0.19% 118 67563 0.20% 119 65375 0.19% 120 68261 0.20% 121 69243 0.20% 122 83260 0.24% 123 84819 0.25% 124 87344 0.26% 125 87445 0.26% 126 80748 0.24% 127 81339 0.24% 128 81170 0.24% 129 87548 0.26% 130 86897 0.26% 131 94760 0.28% 132 91253 0.27% 133 83958 0.25% 134 94315 0.28% 135 91213 0.27% 136 96654 0.28% 137 93110 0.27% 138 103886 0.31% 139 101628 0.30% 140 96435 0.28% 141 115615 0.34% 142 102118 0.30% 143 109573 0.32% 144 109380 0.32% 145 125613 0.37% 146 136752 0.40% 147 147132 0.43% 148 184799 0.54% 149 269866 0.79% 150 2023440 5.95% 151 26861107 79.04% 33984910 reads passed initial QC criterion=sequence-density sequence-density=0.38 sequence-density-rank=1 fanout-score=5.42 fanout-score-rank=19 prefix-density=1.20 prefix-fanout=1.7 sequence=CAGCCTCACGCGGTGCCTGCCGCTCTAGGATCCGTGAGGCCCAGCTTTCGCATAGGCCCCAGCAGATCCACTACGCAA criterion=fanout-score sequence-density=0.01 sequence-density-rank=43 fanout-score=33.85 fanout-score-rank=1 prefix-density=0.20 prefix-fanout=1.1 sequence=GCACTCATCTTGGGGTGGGCTTACTACTTATATGCTTTCAGCAGTTATCCTCTCCGCACTTGGCTACCCAGCGTTTACCGTAGGCACGATAACTGGTACACCAGAGGTGCGTCCTTCCCGGTCCTCTCGTACTAGGGAAAGGTCCTCTCAATGCTCTAACGCCCACACCGGATATGGACCGAACTGTCTCACGACGTTCTGAACCCAGCTCACGTACCGCATTAATGGGCGAACAGCCCAACCCTTGGAACCACCTACAGCTCCAGGTGGCGAAGAGCCGACATCGAGGTGCCAAACCTTCCCGTCGATGTGGACTCTTGGGGAAGATCAGCCTGTTATCCCTAGAGTAACTTTTATCCGTTGAGCGACGGCCCTTCCACTCGGCACCGTCGGATCACTAAGGCCGACTTTCGTCTCTGCTCGACGGGTGAGTCTTGCAGTCAAGCTCCCTTCTGCCTTTGCACTCGAGGACCAATGTCCGTCTGGCCCGAGGAAACCTTTGCACGCCTCC criterion=sequence-density sequence-density=0.55 sequence-density-rank=1 fanout-score=1.94 fanout-score-rank=29 prefix-density=0.55 prefix-fanout=1.9 sequence=GCGTGAGGCTGG criterion=fanout-score sequence-density=0.01 sequence-density-rank=32 fanout-score=21.55 fanout-score-rank=1 prefix-density=0.04 prefix-fanout=5.3 sequence=TTTTTTTTTATGAGATTTTTGCTAAAGTTTCATTTACGCCTAATTCACATCGAGTAGACCTTGTTATTGTGAGAATTCTTAATTCAAGAGTTGTAAGGAGGGACTTATGTCACCACAAACAGAAACTAAAGCAAGTGTTGGATTTAAAGCTGGTGTTAAAGATTATAGATTGACTTACTACACCCCGGAGTATGAAACCAAGGATACTGATATCTTGGCAGCATTCCGAGTATCTCCTCAACCTGGGGTTCCGCCCGAAGAAGCAGGGGCTGCAGTAGCTGCCGAATCTTCTACTGGTACATGGACAACTGTTTGGACTGATGGACTTACTAGTCTTGATCGTTACAAAGGACGATGCTATCACATCGAGCCTGTTCCTGGGGAAGACAGTCAATGGATCTGTTATGTAGCTTATCCATTAGATCTATTTGAAGAGGGTTCCGTTACTAACATGTTTACTTCCATTGTAGGTAACGTATTTGGTTTCAAAGCCCTACGTGCTCTACGTCTG SRR6941556 testing PE reads STAR mapping to Ensembl genome Started job on | Dec 06 11:17:21 Started mapping on | Dec 06 11:17:21 Finished on | Dec 06 11:20:18 Mapping speed, Million of reads per hour | 691.22 Number of input reads | 33984910 Average input read length | 293 UNIQUE READS: Uniquely mapped reads number | 19961325 Uniquely mapped reads % | 58.74% Average mapped length | 294.31 Number of splices: Total | 4148460 Number of splices: Annotated (sjdb) | 3662703 Number of splices: GT/AG | 3849021 Number of splices: GC/AG | 49466 Number of splices: AT/AC | 15769 Number of splices: Non-canonical | 234204 Mismatch rate per base, % | 0.27% Deletion rate per base | 0.02% Deletion average length | 2.03 Insertion rate per base | 0.02% Insertion average length | 2.63 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 10379862 % of reads mapped to multiple loci | 30.54% Number of reads mapped to too many loci | 203828 % of reads mapped to too many loci | 0.60% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 7.45% % of reads unmapped: other | 2.67% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 3663751 3663751 3663751 N_multimapping 10379862 10379862 10379862 N_noFeature 9147759 19316436 9457850 N_ambiguous 642983 7105 317772 UnstrandedReadsAssigned:10170583 PositiveStrandReadsAssigned:637784 NegativeStrandReadsAssigned:10185703 Dataset is classified negative stranded MeadianReadLen=151 20thPercentileLength=151 echo kmer=147 SRR6941556 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,972 [index] number of k-mers: 66,720,672 [index] number of equivalence classes: 111,837 [quant] running in paired-end mode [quant] will process pair 1: SRR6941556-trimmed-pair1.fastq SRR6941556-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 33,984,910 reads, 17,021,475 reads pseudoaligned [quant] estimated average fragment length: 231.488 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,112 rounds 52973 SRR6941556.ke.tsv 35125 SRR6941556.se.tsv 88098 total ==> SRR6941556.ke.tsv <== target_id length eff_length est_counts tpm PNS24245 936 705.91 0 0 PNS24247 1044 813.512 20.8907 1.32192 PNS24249 1928 1697.51 22.6 0.685349 PNS24246 1044 813.512 20.8907 1.32192 PNS24248 1044 813.512 20.8907 1.32192 PNS24244 1471 1240.51 51.7279 2.14655 PNS24243 293 102.186 0 0 KQK14069 1603 1372.51 3039.25 113.99 KQK14071 474 252.514 63.0575 12.8549 ==> SRR6941556.se.tsv <== BRADI_1g14170v3 4016 BRADI_1g53295v3 55 BRADI_1g59795v3 95 BRADI_1g07683v3 0 BRADI_1g00485v3 1 BRADI_1g20270v3 185 BRADI_1g74790v3 4 BRADI_1g09890v3 1 BRADI_1g77505v3 96 BRADI_1g48960v3 0 SRR6941556 completed mapping pipeline successfully