Starting /dee2/code/volunteer_pipeline.sh SRR6941558 current disk space = 1551380766720 free memory = 1604624192 SRR6941558 SRAfilesize 0b00a9a0bf6ee98afd6fbd50c65f72fd SRR6941558.sra SRR6941558.sra file validated SRR6941558 is paired end SRR6941558 is conventional basespace SRR6941558 read1 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR6941558_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 34.52375 35.0 35.0 35.0 34.0 35.0 2 34.54375 35.0 35.0 35.0 35.0 35.0 3 34.563 35.0 35.0 35.0 34.0 35.0 4 34.6495 35.0 35.0 35.0 35.0 35.0 5 34.4665 35.0 35.0 35.0 34.0 35.0 6 39.37375 40.0 40.0 40.0 39.0 40.0 7 39.33425 40.0 40.0 40.0 39.0 40.0 8 39.27275 40.0 40.0 40.0 39.0 40.0 9 39.32975 40.0 40.0 40.0 39.0 40.0 10-14 39.369150000000005 40.0 40.0 40.0 39.0 40.0 15-19 39.2133 40.0 40.0 40.0 38.6 40.0 20-24 39.34625 40.0 40.0 40.0 39.0 40.0 25-29 39.29835 40.0 40.0 40.0 39.0 40.0 30-34 39.23905 40.0 40.0 40.0 38.8 40.0 35-39 39.2838 40.0 40.0 40.0 39.0 40.0 40-44 39.28830000000001 40.0 40.0 40.0 39.0 40.0 45-49 39.21395 40.0 40.0 40.0 38.6 40.0 50-54 39.17615 40.0 40.0 40.0 38.8 40.0 55-59 39.1996 40.0 40.0 40.0 38.8 40.0 60-64 39.1488 40.0 40.0 40.0 38.0 40.0 65-69 39.21955 40.0 40.0 40.0 39.0 40.0 70-74 39.1403 40.0 40.0 40.0 38.4 40.0 75-79 39.0946 40.0 39.8 40.0 38.0 40.0 80-84 39.052 40.0 40.0 40.0 38.0 40.0 85-89 38.91395 40.0 39.0 40.0 37.2 40.0 90-94 38.9663 40.0 39.2 40.0 37.6 40.0 95-99 38.805 40.0 39.0 40.0 36.8 40.0 100-104 38.22855 39.4 38.6 39.8 35.6 39.8 105-109 38.82755000000001 40.0 39.0 40.0 37.0 40.0 110-114 38.856 40.0 39.0 40.0 37.2 40.0 115-119 38.845150000000004 40.0 39.0 40.0 36.8 40.0 120-124 38.6984 40.0 39.0 40.0 36.4 40.0 125-129 38.500299999999996 40.0 39.0 40.0 35.8 40.0 130-134 38.4258 40.0 39.0 40.0 35.8 40.0 135-139 38.351099999999995 40.0 39.0 40.0 35.8 40.0 140-144 38.23805 40.0 39.0 40.0 35.6 40.0 145-149 37.78054999999999 40.0 38.8 40.0 34.6 40.0 150-151 35.148875000000004 38.5 35.0 39.5 25.0 40.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 22 1.0 23 3.0 24 4.0 25 1.0 26 14.0 27 11.0 28 12.0 29 12.0 30 30.0 31 32.0 32 44.0 33 56.0 34 59.0 35 91.0 36 104.0 37 176.0 38 377.0 39 2973.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 60.32581453634085 8.922305764411027 3.3583959899749374 27.39348370927318 2 22.932330827067666 8.897243107769423 31.152882205513784 37.01754385964912 3 22.575 18.55 23.875 35.0 4 26.174999999999997 24.224999999999998 20.65 28.95 5 24.58521870286576 32.05128205128205 23.881347410759176 19.482151835093013 6 21.75 35.375 20.474999999999998 22.400000000000002 7 13.675 30.349999999999998 38.475 17.5 8 16.950000000000003 27.825 31.775 23.45 9 18.65 22.75 35.35 23.25 10-14 20.615 31.39 25.729999999999997 22.264999999999997 15-19 20.705000000000002 30.25 24.959999999999997 24.085 20-24 18.64 30.354999999999997 26.165 24.84 25-29 22.03 29.270000000000003 26.479999999999997 22.220000000000002 30-34 22.134999999999998 31.374999999999996 24.75 21.740000000000002 35-39 21.834999999999997 28.185 26.875 23.105 40-44 18.915000000000003 28.749999999999996 27.265 25.069999999999997 45-49 20.724999999999998 27.560000000000002 29.015 22.7 50-54 21.625 29.220000000000002 25.115 24.04 55-59 20.345 28.205000000000002 26.715 24.735 60-64 19.425 28.475 27.185 24.915000000000003 65-69 20.185 30.11 26.195 23.51 70-74 20.105 28.935 25.180000000000003 25.779999999999998 75-79 21.01 28.310000000000002 27.05 23.630000000000003 80-84 22.125 29.225 25.86 22.79 85-89 21.279999999999998 28.64 25.945 24.135 90-94 19.98 29.549999999999997 26.0 24.47 95-99 21.02 30.15 24.25 24.58 100-104 21.17 30.595 24.29 23.945 105-109 20.755000000000003 29.354999999999997 24.775 25.115 110-114 21.91 27.615000000000002 24.8 25.674999999999997 115-119 20.955 31.105 23.385 24.555 120-124 22.105 29.79 22.79 25.314999999999998 125-129 21.945 29.03 23.935000000000002 25.09 130-134 22.67 29.054999999999996 23.115 25.16 135-139 21.915000000000003 29.349999999999998 24.72 24.015 140-144 23.5 29.685 24.310000000000002 22.505 145-149 21.060000000000002 29.92 23.385 25.635 150-151 21.40082696403959 29.695526876331286 22.841749154241324 26.061897005387795 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.5 6 0.5 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.5 20 1.5 21 1.5 22 2.5 23 3.0 24 3.0 25 3.5 26 3.5 27 8.5 28 13.5 29 14.5 30 14.5 31 23.5 32 32.0 33 32.5 34 32.5 35 39.5 36 72.5 37 147.0 38 228.0 39 289.5 40 279.0 41 262.5 42 234.0 43 201.0 44 204.0 45 188.5 46 149.5 47 121.0 48 112.5 49 85.5 50 77.0 51 79.0 52 76.0 53 81.0 54 97.5 55 127.0 56 137.0 57 101.0 58 95.5 59 99.5 60 63.0 61 37.0 62 29.0 63 23.0 64 19.0 65 14.0 66 10.0 67 6.0 68 7.0 69 4.5 70 2.0 71 3.5 72 3.0 73 1.0 74 0.5 75 1.0 76 0.5 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.25 2 0.25 3 0.0 4 0.0 5 0.5499999999999999 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.2375 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.150000000000006 #Duplication Level Percentage of deduplicated Percentage of total 1 83.64677023712184 51.15 2 8.462796402289452 10.35 3 2.3303352412101392 4.275 4 1.553556827473426 3.8 5 0.8994276369582993 2.75 6 0.6132461161079313 2.25 7 0.3270645952575634 1.4000000000000001 8 0.2861815208503679 1.4000000000000001 9 0.24529844644317253 1.35 >10 1.5126737530662304 16.425 >50 0.12264922322158626 4.8500000000000005 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTGCTCTGCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTCCTA 73 1.825 No Hit GGCCAAAATAACCATGAGCAGCCACAATATTATAAGTCTCTTCCTCTTGA 67 1.675 No Hit GCGGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTACG 54 1.35 No Hit GCCGAAAACACCAGCTACACCTAACATGTGAAATGGATGCATAAGGATGT 41 1.0250000000000001 No Hit GTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTC 40 1.0 No Hit GCTCTGCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTCCTAAA 38 0.95 No Hit GCGACCTTGACTATCAACTACAGATTGGTTGAAATTGAATCCATTTAGGT 36 0.8999999999999999 No Hit GCCAAAATAACCATGAGCAGCCACAATATTATAAGTCTCTTCCTCTTGAC 35 0.8750000000000001 No Hit GCGGAAAAACCTTATCCATTAATAGCGGGAACTTCAAGAGCAGCTAGATC 24 0.6 No Hit GGATGCATAAGGATGTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAA 23 0.575 No Hit CTCTGCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTCCTAAAG 22 0.5499999999999999 No Hit GTGAGCATTACGTTCGTGCATTACTTCCATACCAAGATTAGCACGGTTGA 20 0.5 No Hit CTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCATTAAT 19 0.475 No Hit GCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTCCTAAAGGCAT 19 0.475 No Hit GCGGTCAATAAGGTAGGGATCATCAAAACACCGAACCATCCGATGTAAAG 18 0.44999999999999996 No Hit GGCATGATGACTTGGCCTCATCCTCTCCTTCCTCCGGCTTAACACCGGCG 18 0.44999999999999996 No Hit GGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTACGTT 18 0.44999999999999996 No Hit GGAGGGGCTGCGATGAAGGCGATAATAAATACAGAAGTTGCGGTCAATAA 17 0.42500000000000004 No Hit CCCCACTGCTGCCTCCCGTAGGAGTCTGGGCCGTGTCTCAGTCCCAGTGT 16 0.4 No Hit GCACGGTTGATGATATCAGCCCAAGTATTAATAACGCGACCTTGACTATC 15 0.375 No Hit GGCGATAATAAATACAGAAGTTGCGGTCAATAAGGTAGGGATCATCAAAA 15 0.375 No Hit GCTTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCA 15 0.375 No Hit GTTGAAACTAGCATATTGGAAGATTAATCGGCCAAAATAACCATGAGCAG 15 0.375 No Hit CTTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCAT 14 0.35000000000000003 No Hit CCCACTGCTGCCTCCCGTAGGAGTCTGGGCCGTGTCTCAGTCCCAGTGTG 13 0.325 No Hit GTTAGCGGAAAAACCTTATCCATTAATAGCGGGAACTTCAAGAGCAGCTA 13 0.325 No Hit GTCGGTTTCGGGTACAGGTACCCTTTTGTTGAAGGTCGTTCGAGCTTTTC 12 0.3 No Hit CCCTCATTAGCAGATTCATTTTCAGTAGTTTCCCTGATCAAACTAGAGGT 12 0.3 No Hit CCACAATATTATAAGTCTCTTCCTCTTGACCAAATTTGTAACCCTCATTA 12 0.3 No Hit GCATAAGGATGTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAAAGTA 12 0.3 No Hit CCCAAGTATTAATAACGCGACCTTGACTATCAACTACAGATTGGTTGAAA 11 0.27499999999999997 No Hit GCTGCGATGAAGGCGATAATAAATACAGAAGTTGCGGTCAATAAGGTAGG 11 0.27499999999999997 No Hit GTTGATGATATCAGCCCAAGTATTAATAACGCGACCTTGACTATCAACTA 11 0.27499999999999997 No Hit CCGGGAACGGATTCACCGCCGTATGGCTGACCGGCGATTACTAGCGATTC 11 0.27499999999999997 No Hit GCCAAGAAGAAGTGTAAAGAACGAGAGTTGTTGAAACTAGCATATTGGAA 11 0.27499999999999997 No Hit GGGAAGTTGTGAGCATTACGTTCGTGCATTACTTCCATACCAAGATTAGC 10 0.25 No Hit GTCCCAGTGTGGCTGATCATCCTCTCGGACCAGCTACTGATCATCGCCTT 10 0.25 No Hit GTCCAGTCAACTGCTGCGCCTCAACGCATTTCGGGGAGAACCAGCTAGCT 10 0.25 No Hit CGCGACCTTGACTATCAACTACAGATTGGTTGAAATTGAATCCATTTAGG 10 0.25 No Hit GGGGCTGCGATGAAGGCGATAATAAATACAGAAGTTGCGGTCAATAAGGT 10 0.25 No Hit CCTCAGCCTACGGGGTATTAGCAACCGTTTCCAGTTGTTGTTCCCCTCCC 9 0.22499999999999998 No Hit GGAAAAACCTTATCCATTAATAGCGGGAACTTCAAGAGCAGCTAGATCTA 9 0.22499999999999998 No Hit GGCGCATACCCAAACGGAAACTAAGTTCCCACTCACGACCCATATAACAA 9 0.22499999999999998 No Hit GCTAGTTATCCAGTTGCAGAAGCGACCCCACAGGCTTGTACTTTCGCGTC 9 0.22499999999999998 No Hit GGTCAATAAGGTAGGGATCATCAAAACACCGAACCATCCGATGTAAAGAC 9 0.22499999999999998 No Hit ACGCGACCTTGACTATCAACTACAGATTGGTTGAAATTGAATCCATTTAG 9 0.22499999999999998 No Hit CGGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTACGT 8 0.2 No Hit GTAGGGATCATCAAAACACCGAACCATCCGATGTAAAGACGATTTTCAGT 8 0.2 No Hit GCCCAATCATTCCGGATAACGCTTGCATCCTCTGTCTTACCGCGGCTGCT 8 0.2 No Hit GGCCAAGCAGCCAAGAAGAAGTGTAAAGAACGAGAGTTGTTGAAACTAGC 8 0.2 No Hit GGTCGTTCGAGCTTTTCCTGGGAGTATGGCATCGGTTACATACTTCAGTG 8 0.2 No Hit CTCCACGCGGCATTGCTCCGTCAGGCTTTCGCCCATTGCGGAAAATTCCC 8 0.2 No Hit CCATGAGCAGCCACAATATTATAAGTCTCTTCCTCTTGACCAAATTTGTA 8 0.2 No Hit CCGCAGAAGTAGGAATAATAGCACCAGAGATAATATTGTTTCCATAAAGT 7 0.17500000000000002 No Hit ACCAGATATTCCTAAAGGCATACCATCAGAGAAGCTTCCTTGACCAATAG 7 0.17500000000000002 No Hit GCATTACGTTCGTGCATTACTTCCATACCAAGATTAGCACGGTTGATGAT 7 0.17500000000000002 No Hit GTACAAGGCCCGGGAACGGATTCACCGCCGTATGGCTGACCGGCGATTAC 7 0.17500000000000002 No Hit GTGCTAGTTATCCAGTTGCAGAAGCGACCCCACAGGCTTGTACTTTCGCG 7 0.17500000000000002 No Hit GATGCATAAGGATGTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAAA 7 0.17500000000000002 No Hit GTCCATGTACCAGTAGAAGATTCGGCAGCTACTGCAGCCCCTGCTTCTTC 7 0.17500000000000002 No Hit AGCAGCCACAATATTATAAGTCTCTTCCTCTTGACCAAATTTGTAACCCT 7 0.17500000000000002 No Hit GCGATAATAAATACAGAAGTTGCGGTCAATAAGGTAGGGATCATCAAAAC 6 0.15 No Hit GCGATGAAGGCGATAATAAATACAGAAGTTGCGGTCAATAAGGTAGGGAT 6 0.15 No Hit GTCATTGTTTCTTCTCCGAGAAAAGAAGTTGACGACCCGTAGGCCTTCCA 6 0.15 No Hit GGGGCATGATGACTTGGCCTCATCCTCTCCTTCCTCCGGCTTAACACCGG 6 0.15 No Hit ATAAGGATGTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAAAGTACC 6 0.15 No Hit CCAGATTCCTACTACAGGCCAAGCAGCCAAGAAGAAGTGTAAAGAACGAG 6 0.15 No Hit GGATCATCAAAACACCGAACCATCCGATGTAAAGACGATTTTCAGTGCTA 6 0.15 No Hit GCTACACCTAACATGTGAAATGGATGCATAAGGATGTTGTGCTCTGCCTG 6 0.15 No Hit GTAAAGAACGAGAGTTGTTGAAACTAGCATATTGGAAGATTAATCGGCCA 6 0.15 No Hit CCCGTCAATTCCTTTGAGTTTCATTCTTGCGAACGTACTCCCCAGGCGGG 6 0.15 No Hit CCAAGTATTAATAACGCGACCTTGACTATCAACTACAGATTGGTTGAAAT 6 0.15 No Hit GTTTCCATAAAGTAAAGAACCAGAAACAGGCTCGCGAATACCATCAATAT 6 0.15 No Hit CTAGCTTTCGTCTCTCAGTGTCAGTGTCGGCCCAGCAGAGTGCTTTCGCC 6 0.15 No Hit GCAAGAAATAAATGAATTCCAAAGATCTTGGGCAAATCTAAAGAGGGTTT 6 0.15 No Hit GTCAATAAGGTAGGGATCATCAAAACACCGAACCATCCGATGTAAAGACG 6 0.15 No Hit CCACTGCTGCCTCCCGTAGGAGTCTGGGCCGTGTCTCAGTCCCAGTGTGG 5 0.125 No Hit ACCAGCTACACCTAACATGTGAAATGGATGCATAAGGATGTTGTGCTCTG 5 0.125 No Hit CGCAGAAGTAGGAATAATAGCACCAGAGATAATATTGTTTCCATAAAGTA 5 0.125 No Hit GGAAGATTAATCGGCCAAAATAACCATGAGCAGCCACAATATTATAAGTC 5 0.125 No Hit ATTACGTTCGTGCATTACTTCCATACCAAGATTAGCACGGTTGATGATAT 5 0.125 No Hit GTCTCTTCCTCTTGACCAAATTTGTAACCCTCATTAGCAGATTCATTTTC 5 0.125 No Hit GGGTAATGTTGCTCCAATACCTAACCAAAGAGCTACTGCAGTACCGATTA 5 0.125 No Hit TGATGATATCAGCCCAAGTATTAATAACGCGACCTTGACTATCAACTACA 5 0.125 No Hit TCTGCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTCCTAAAGG 5 0.125 No Hit GCCATAGTACTAATACCTAAAGCAGTGAACCAGATTCCTACTACAGGCCA 5 0.125 No Hit CCTCACGGTACTACTTCGCTATCGGTCACCCAGGAGTATTTAGCCTTGCA 5 0.125 No Hit GCCACCTACAGACGCTTTACGCCCAATCATTCCGGATAACGCTTGCATCC 5 0.125 No Hit GGATGTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAAAGTACCAGAT 5 0.125 No Hit CCTAGCTTTCGTCTCTCAGTGTCAGTGTCGGCCCAGCAGAGTGCTTTCGC 5 0.125 No Hit CTCATTAGCAGATTCATTTTCAGTAGTTTCCCTGATCAAACTAGAGGTTA 5 0.125 No Hit CGTCATTGTTTCTTCTCCGAGAAAAGAAGTTGACGACCCGTAGGCCTTCC 5 0.125 No Hit CTCTTGACCAAATTTGTAACCCTCATTAGCAGATTCATTTTCAGTAGTTT 5 0.125 No Hit GCCCAAGTATTAATAACGCGACCTTGACTATCAACTACAGATTGGTTGAA 5 0.125 No Hit GGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTACGTTC 5 0.125 No Hit GCTCCTCAGCCTACGGGGTATTAGCAACCGTTTCCAGTTGTTGTTCCCCT 5 0.125 No Hit CACCATTGTATAACCATTCATCAACGGATGCAGCTTCCCAAATTGGGTAA 5 0.125 No Hit GACCCATATAACAAGCTACACCAAGTAAGAAGTGTAGAACAATTAGCTCA 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0125 0.0 0.0 0.0 0.0 58-59 0.025 0.0 0.0 0.0 0.0 60-61 0.025 0.0 0.0 0.0 0.0 62-63 0.05 0.0 0.0 0.0 0.0 64-65 0.05 0.0 0.0 0.0 0.0 66-67 0.05 0.0 0.0 0.0 0.0 68-69 0.0625 0.0 0.0 0.0 0.0 70-71 0.1375 0.0 0.0 0.0 0.0 72-73 0.2875 0.0 0.0 0.0 0.0 74-75 0.3875 0.0 0.0 0.0 0.0 76-77 0.4375 0.0 0.0 0.0 0.0 78-79 0.55 0.0 0.0 0.0 0.0 80-81 0.6499999999999999 0.0 0.0 0.0 0.0 82-83 0.7875 0.0 0.0 0.0 0.0 84-85 0.9125 0.0 0.0 0.0 0.0 86-87 1.0875 0.0 0.0 0.0 0.0 88-89 1.275 0.0 0.0 0.0 0.0 90-91 1.4500000000000002 0.0 0.0 0.0 0.0 92-93 1.7000000000000002 0.0 0.0 0.0 0.0 94-95 2.0625 0.0 0.0 0.0 0.0 96-97 2.175 0.0 0.0 0.0 0.0 98-99 2.4625 0.0 0.0 0.0 0.0 100-101 2.7875 0.0 0.0 0.0 0.0 102-103 3.15 0.0 0.0 0.0 0.0 104-105 3.5875000000000004 0.0 0.0 0.0 0.0 106-107 3.95 0.0 0.0 0.0 0.0 108-109 4.2625 0.0 0.0 0.0 0.0 110-111 4.6375 0.0 0.0 0.0 0.0 112-113 5.075 0.0 0.0 0.0 0.0 114-115 5.4625 0.0 0.0 0.0 0.0 116-117 5.975 0.0 0.0 0.0 0.0 118-119 6.425 0.0 0.0 0.0 0.0 120-121 6.975 0.0 0.0 0.0 0.0 122-123 7.4375 0.0 0.0 0.0 0.0 124-125 7.824999999999999 0.0 0.0 0.0 0.0 126-127 8.2625 0.0 0.0 0.0 0.0 128-129 8.65 0.0 0.0 0.0 0.0 130-131 9.1 0.0 0.0 0.0 0.0 132-133 9.7625 0.0 0.0 0.0 0.0 134-135 10.4 0.0 0.0 0.0 0.0 136-137 11.1875 0.0 0.0 0.0 0.0 138-139 11.95 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TTGTGCT 20 3.4162842E-4 110.06962 2 TGTGCTC 20 3.4162842E-4 110.06962 3 GTTGTGC 20 3.4162842E-4 110.06962 1 GGCCAAA 45 7.681592E-9 97.83966 1 AACCGCC 40 4.7075991E-7 91.724686 145 TGCTCTG 25 8.2975137E-4 88.0557 5 GCCAAAA 50 1.5974365E-8 88.0557 2 AAATAAC 50 1.7491402E-8 86.955 6 CCAAAAT 55 3.09592E-8 80.05064 3 AAAATAA 55 3.09592E-8 80.05064 5 CAAAATA 55 3.09592E-8 80.05064 4 TAACCAT 55 3.3900506E-8 79.049995 9 AATAACC 55 3.3900506E-8 79.049995 7 AATCCAA 30 0.0017116986 73.379745 145 GAGCATT 30 0.0017116986 73.379745 3 GTGAGCA 30 0.0017116986 73.379745 1 AGCATTA 30 0.0017116986 73.379745 4 GTGCTCT 30 0.0017116986 73.379745 4 ATAACCA 60 6.199116E-8 72.4625 8 GCTCTGC 30 0.001801043 72.4625 6 >>END_MODULE SRR6941558 read2 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR6941558_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 34.0885 35.0 35.0 35.0 32.0 35.0 2 34.24725 35.0 35.0 35.0 33.0 35.0 3 34.3375 35.0 35.0 35.0 33.0 35.0 4 34.241 35.0 35.0 35.0 33.0 35.0 5 34.29425 35.0 35.0 35.0 33.0 35.0 6 38.9895 40.0 40.0 40.0 38.0 40.0 7 38.8705 40.0 40.0 40.0 38.0 40.0 8 39.00125 40.0 40.0 40.0 38.0 40.0 9 39.03075 40.0 40.0 40.0 39.0 40.0 10-14 39.0252 40.0 40.0 40.0 38.6 40.0 15-19 39.08395 40.0 40.0 40.0 39.0 40.0 20-24 39.037549999999996 40.0 40.0 40.0 38.4 40.0 25-29 39.004900000000006 40.0 40.0 40.0 38.4 40.0 30-34 38.94715 40.0 39.8 40.0 38.2 40.0 35-39 39.00834999999999 40.0 40.0 40.0 38.2 40.0 40-44 38.90915 40.0 40.0 40.0 37.8 40.0 45-49 38.687599999999996 40.0 39.2 40.0 36.8 40.0 50-54 38.684799999999996 40.0 39.0 40.0 36.8 40.0 55-59 38.75359999999999 40.0 39.2 40.0 37.6 40.0 60-64 38.68325 40.0 39.0 40.0 36.8 40.0 65-69 38.572950000000006 40.0 39.0 40.0 36.6 40.0 70-74 38.621050000000004 40.0 39.0 40.0 36.4 40.0 75-79 38.39425 40.0 39.0 40.0 35.8 40.0 80-84 38.372099999999996 40.0 39.0 40.0 36.0 40.0 85-89 38.39205 40.0 39.0 40.0 36.0 40.0 90-94 38.3197 40.0 39.0 40.0 36.0 40.0 95-99 38.21300000000001 40.0 39.0 40.0 35.8 40.0 100-104 37.3408 39.2 38.0 39.6 33.2 39.8 105-109 37.9595 40.0 39.0 40.0 35.2 40.0 110-114 34.5492 36.4 34.2 38.4 29.2 38.6 115-119 18.668799999999997 16.6 15.8 23.4 13.4 30.0 120-124 2.0 2.0 2.0 2.0 2.0 2.0 125-129 2.0 2.0 2.0 2.0 2.0 2.0 130-134 2.0 2.0 2.0 2.0 2.0 2.0 135-139 2.0 2.0 2.0 2.0 2.0 2.0 140-144 2.0 2.0 2.0 2.0 2.0 2.0 145-149 2.0 2.0 2.0 2.0 2.0 2.0 150-151 2.0 2.0 2.0 2.0 2.0 2.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6.0 3 3.0 4 1.0 5 0.0 6 1.0 7 0.0 8 3.0 9 1.0 10 1.0 11 0.0 12 4.0 13 3.0 14 3.0 15 6.0 16 2.0 17 9.0 18 4.0 19 5.0 20 20.0 21 23.0 22 25.0 23 42.0 24 39.0 25 44.0 26 65.0 27 125.0 28 162.0 29 316.0 30 1962.0 31 1125.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 53.77287540737027 17.999498621208325 9.651541739784406 18.576084231637 2 24.893563736538944 19.834710743801654 33.25820185324317 22.01352366641623 3 21.707561342013022 22.759138708062093 38.98347521281923 16.54982473710566 4 25.820185324317556 29.97746055597295 24.74330077635863 19.459053343350863 5 29.151014274981218 31.905835211620335 20.96168294515402 17.981467568244426 6 20.974999999999998 36.375 24.75 17.9 7 20.05 21.075 40.25 18.625 8 20.674999999999997 25.174999999999997 29.45 24.7 9 23.3 22.0 31.724999999999998 22.975 10-14 24.895 25.35 29.715000000000003 20.04 15-19 24.345 24.95 29.965000000000003 20.74 20-24 24.39 25.074999999999996 30.44 20.095 25-29 24.625 25.3 29.115000000000002 20.96 30-34 24.935 24.92 29.835 20.31 35-39 25.15751575157516 24.832483248324834 29.832983298329836 20.17701770177018 40-44 24.589917983596717 25.670134026805364 29.38087617523505 20.35907181436287 45-49 24.05412177399148 25.943372588323726 29.391130042595844 20.611375595088948 50-54 24.56588099884902 24.65595756392934 29.85037281689436 20.927788620327277 55-59 24.647111823005304 26.338972870157175 28.436279907898687 20.57763539893883 60-64 24.3383861123618 24.40842463354845 29.986492570914002 21.266696683175745 65-69 24.06703351675838 25.972986493246626 28.854427213606805 21.105552776388194 70-74 25.087543771885944 25.827913956978488 28.649324662331168 20.4352176088044 75-79 25.20378056708506 24.768715307296095 29.204380657098568 20.823123468520276 80-84 24.622235564895426 24.26198338837186 31.011708195737015 20.104072850995696 85-89 25.50755075507551 24.992499249924993 28.882888288828884 20.617061706170617 90-94 24.745 25.195 28.444999999999997 21.615000000000002 95-99 24.349999999999998 24.45 29.675 21.525 100-104 24.73623681184059 25.896294814740738 29.181459072953647 20.186009300465024 105-109 25.935000000000002 24.695 29.125 20.244999999999997 110-114 25.514856942426217 25.595029313022998 28.65661171518765 20.23350202936313 115-119 25.527903469079938 26.23177476118653 28.01659125188537 20.223730517848164 120-124 25.631080307751002 40.55446048197368 17.40319196286641 16.411267247408915 125-129 NaN NaN NaN NaN 130-134 25.08483096644464 26.291315822546185 28.490637174814626 20.133216036194547 135-139 25.3779317964944 25.629049269248156 28.84335292049621 20.149666013761237 140-144 26.233963218100232 25.81684889085508 29.469759211274727 18.479428679769956 145-149 25.69427070302727 25.719289467100324 29.997498123592692 18.58894170627971 150-151 25.156602355299423 25.031320471059885 30.76923076923077 19.04284640440992 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 1.0 12 1.0 13 0.0 14 0.5 15 0.5 16 0.0 17 0.0 18 0.5 19 0.5 20 0.0 21 2.5 22 4.0 23 4.0 24 5.0 25 5.5 26 8.5 27 12.0 28 13.0 29 16.0 30 22.5 31 27.5 32 28.0 33 38.0 34 61.0 35 78.5 36 94.0 37 129.5 38 152.5 39 186.5 40 219.0 41 216.5 42 195.0 43 213.5 44 237.5 45 203.0 46 192.0 47 164.0 48 127.0 49 102.5 50 84.5 51 94.5 52 78.0 53 79.0 54 128.0 55 148.5 56 126.0 57 85.5 58 63.0 59 62.5 60 59.5 61 55.5 62 53.0 63 38.5 64 17.5 65 10.5 66 9.5 67 10.5 68 9.0 69 5.0 70 3.5 71 3.5 72 3.5 73 2.0 74 2.0 75 1.5 76 1.5 77 1.0 78 0.5 79 0.5 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 0.27499999999999997 2 0.17500000000000002 3 0.15 4 0.17500000000000002 5 0.17500000000000002 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.01 40-44 0.02 45-49 0.22499999999999998 50-54 0.08499999999999999 55-59 0.11 60-64 0.055 65-69 0.05 70-74 0.05 75-79 0.015 80-84 0.06999999999999999 85-89 0.01 90-94 0.0 95-99 0.0 100-104 0.005 105-109 0.0 110-114 0.215 115-119 20.44 120-124 21.365000000000002 125-129 100.0 130-134 60.214999999999996 135-139 0.445 140-144 20.885 145-149 80.015 150-151 0.22499999999999998 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 65.325 #Duplication Level Percentage of deduplicated Percentage of total 1 81.20933792575583 53.05 2 9.223115193264448 12.049999999999999 3 3.9418293149636434 7.725 4 1.8752391886720245 4.9 5 1.0715652506697282 3.5000000000000004 6 0.5740528128587831 2.25 7 0.4975124378109453 2.275 8 0.30616150019135097 1.6 9 0.30616150019135097 1.7999999999999998 >10 0.9950248756218906 10.85 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GCTTGTTATATGGGTCGTGAGTGGGAACTTAGTTTCCGTTTGGGTATGCG 31 0.775 No Hit GCACAACATCCTTATGCATCCATTTCACATGTTAGGTGTAGCTGGTGTTT 30 0.75 No Hit GTTCGATCCTGGCTCAGGATGAACGCTGGCGGCATGCTTAACACATGCAA 29 0.7250000000000001 No Hit GTGGGAACTTAGTTTCCGTTTGGGTATGCGCCCTTGGATTGCTGTTGCAT 26 0.65 No Hit GTTATTTTGGCCGATTAATCTTCCAATATGCTAGTTTCAACAACTCTCGT 22 0.5499999999999999 No Hit GTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGATCTTACCATGAC 21 0.525 No Hit GCTAATTGTTCTACACTTCTTACTTGGTGTAGCTTGTTATATGGGTCGTG 18 0.44999999999999996 No Hit GCTGCATCCGTTGATGAATGGTTATACAATGGTGGTCCTTATGAGCTAAT 18 0.44999999999999996 No Hit GCTGCGACTGCTGTTTTCTTGATTTACCCTATTGGTCAAGGAAGCTTCTC 17 0.42500000000000004 No Hit AGCACAACATCCTTATGCATCCATTTCACATGTTAGGTGTAGCTGGTGTT 16 0.4 No Hit GCTAGTTTCAACAACTCTCGTTCTTTACACTTCTTCTTGGCTGCTTGGCC 15 0.375 No Hit GTTGCATATTCAGCTCCTGTTGCAGCTGCGACTGCTGTTTTCTTGATTTA 15 0.375 No Hit GTTTCAACAACTCTCGTTCTTTACACTTCTTCTTGGCTGCTTGGCCTGTA 15 0.375 No Hit GTTAGGTGTAGCTGGTGTTTTCGGCGGTTCCCTATTCAGTGCTATGCATG 15 0.375 No Hit ATTATCTATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAAT 15 0.375 No Hit AGGGAAACTACTGAAAATGAATCTGCTAATGAGGGTTACAAATTTGGTCA 14 0.35000000000000003 No Hit ATCTATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTG 14 0.35000000000000003 No Hit CCTTACCAGGGCTTGACATGCCGCGAATCCTCTTGAAAGAGAGGGGTGCC 13 0.325 No Hit GTTTGATCAGGGAAACTACTGAAAATGAATCTGCTAATGAGGGTTACAAA 13 0.325 No Hit GTCGTGAGTGGGAACTTAGTTTCCGTTTGGGTATGCGCCCTTGGATTGCT 12 0.3 No Hit CTATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAA 12 0.3 No Hit GGAAGAGACTTATAATATTGTGGCTGCTCATGGTTATTTTGGCCGATTAA 11 0.27499999999999997 No Hit GTTATACAATGGTGGTCCTTATGAGCTAATTGTTCTACACTTCTTACTTG 11 0.27499999999999997 No Hit GAGGAAGAGACTTATAATATTGTGGCTGCTCATGGTTATTTTGGCCGATT 11 0.27499999999999997 No Hit GGAAGCTTCTCTGATGGTATGCCTTTAGGAATATCTGGTACTTTCAACTT 10 0.25 No Hit GTAGCTTGTTATATGGGTCGTGAGTGGGAACTTAGTTTCCGTTTGGGTAT 10 0.25 No Hit GCATGGTTCCTTGGTAACCTCTAGTTTGATCAGGGAAACTACTGAAAATG 9 0.22499999999999998 No Hit CTAGCACTGAAAATCGTCTTTACATCGGATGGTTCGGTGTTTTGATGATC 9 0.22499999999999998 No Hit GTTGATGAATGGTTATACAATGGTGGTCCTTATGAGCTAATTGTTCTACA 9 0.22499999999999998 No Hit GTCAAGGTCGCGTTATTAATACTTGGGCTGATATCATCAACCGTGCTAAT 9 0.22499999999999998 No Hit GTTCAACCTAAATGGATTCAATTTCAACCAATCTGTAGTTGATAGTCAAG 9 0.22499999999999998 No Hit TATCTATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTT 9 0.22499999999999998 No Hit GTTCTACACTTCTTACTTGGTGTAGCTTGTTATATGGGTCGTGAGTGGGA 9 0.22499999999999998 No Hit GGTACTTTCAACTTTATGATTGTATTCCAGGCAGAGCACAACATCCTTAT 9 0.22499999999999998 No Hit GCTCATGGTTATTTTGGCCGATTAATCTTCCAATATGCTAGTTTCAACAA 8 0.2 No Hit CTGGTACTTTCAACTTTATGATTGTATTCCAGGCAGAGCACAACATCCTT 8 0.2 No Hit GAACCTTACCAGGGCTTGACATGCCGCGAATCCTCTTGAAAGAGAGGGGT 8 0.2 No Hit GTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGATCTT 8 0.2 No Hit GTAGGAATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCT 8 0.2 No Hit GTTATTGTGAGAATTCTTAATTCAAGAGTTGTAAGGAGGGACTTATGTCA 8 0.2 No Hit GTTGGGTTAAGTCTCGCAACGAGCGCAACCCTCGTGTTTAGTTGCCACTA 8 0.2 No Hit TCTATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGA 8 0.2 No Hit GTTTGGGTATGCGCCCTTGGATTGCTGTTGCATATTCAGCTCCTGTTGCA 7 0.17500000000000002 No Hit ATCGGATTGCACTTTTACCCAATTTGGGAAGCTGCATCCGTTGATGAATG 7 0.17500000000000002 No Hit GCCTGACGGAGCAATGCCGCGTGGAGGTGGAAGGCCTACGGGTCGTCAAC 7 0.17500000000000002 No Hit GGGAAACTACTGAAAATGAATCTGCTAATGAGGGTTACAAATTTGGTCAA 7 0.17500000000000002 No Hit CCTGGCTCAGGATGAACGCTGGCGGCATGCTTAACACATGCAAGTCGAAC 7 0.17500000000000002 No Hit GAGACTTATAATATTGTGGCTGCTCATGGTTATTTTGGCCGATTAATCTT 7 0.17500000000000002 No Hit GTTTTGATGATCCCTACCTTATTGACCGCAACTTCTGTATTTATTATCGC 7 0.17500000000000002 No Hit ATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATA 7 0.17500000000000002 No Hit GGGAACTTAGTTTCCGTTTGGGTATGCGCCCTTGGATTGCTGTTGCATAT 7 0.17500000000000002 No Hit CAGTGCTATGCATGGTTCCTTGGTAACCTCTAGTTTGATCAGGGAAACTA 7 0.17500000000000002 No Hit CCTAGGCACCCAGAGACGAGGAAGGGCGTAGCAAGCGACGAAATGCTTCG 7 0.17500000000000002 No Hit CCTGAACAGACCGCCGGTGTTAAGCCGGAGGAAGGAGAGGATGAGGCCAA 7 0.17500000000000002 No Hit GAGCACAACATCCTTATGCATCCATTTCACATGTTAGGTGTAGCTGGTGT 7 0.17500000000000002 No Hit CAGGGCTTGACATGCCGCGAATCCTCTTGAAAGAGAGGGGTGCCCTCGGG 6 0.15 No Hit GAAGCTGCATCCGTTGATGAATGGTTATACAATGGTGGTCCTTATGAGCT 6 0.15 No Hit GCTTCTCTGATGGTATGCCTTTAGGAATATCTGGTACTTTCAACTTTATG 6 0.15 No Hit GCTTGGGAGTCCTTGCAATTTGAATAAACCAAGATCTTACCATGACTGCA 6 0.15 No Hit CCTAAATGGATTCAATTTCAACCAATCTGTAGTTGATAGTCAAGGTCGCG 6 0.15 No Hit GTACTATGGCGTTCAACCTAAATGGATTCAATTTCAACCAATCTGTAGTT 6 0.15 No Hit GACTTATAATATTGTGGCTGCTCATGGTTATTTTGGCCGATTAATCTTCC 6 0.15 No Hit GTTATATGGGTCGTGAGTGGGAACTTAGTTTCCGTTTGGGTATGCGCCCT 6 0.15 No Hit GGCTTGCGGTGGATACCTAGGCACCCAGAGACGAGGAAGGGCGTAGCAAG 6 0.15 No Hit GCCTTTAGGAATATCTGGTACTTTCAACTTTATGATTGTATTCCAGGCAG 6 0.15 No Hit ATTCAGCTCCTGTTGCAGCTGCGACTGCTGTTTTCTTGATTTACCCTATT 6 0.15 No Hit AGTGACCAGGCCCGGGCGACTGTTTACCAAAAACACAGGTCTCCGCAAAG 6 0.15 No Hit GGTCAAGGAAGCTTCTCTGATGGTATGCCTTTAGGAATATCTGGTACTTT 6 0.15 No Hit CAGGGAAACTACTGAAAATGAATCTGCTAATGAGGGTTACAAATTTGGTC 6 0.15 No Hit GAATGGTTATACAATGGTGGTCCTTATGAGCTAATTGTTCTACACTTCTT 6 0.15 No Hit GTAGGGGCAGAGGGAATTTCCGGTGGAGCGGTGAAATGCATTGAGATCGG 5 0.125 No Hit GGTCGCTTCTGCAACTGGATAACTAGCACTGAAAATCGTCTTTACATCGG 5 0.125 No Hit ATTATTCCTACTTCTGCGGCAATCGGATTGCACTTTTACCCAATTTGGGA 5 0.125 No Hit GGTAACCTCTAGTTTGATCAGGGAAACTACTGAAAATGAATCTGCTAATG 5 0.125 No Hit CGGATTGCACTTTTACCCAATTTGGGAAGCTGCATCCGTTGATGAATGGT 5 0.125 No Hit CTGCAACTGGATAACTAGCACTGAAAATCGTCTTTACATCGGATGGTTCG 5 0.125 No Hit GGTGGTCCTTATGAGCTAATTGTTCTACACTTCTTACTTGGTGTAGCTTG 5 0.125 No Hit GCAACTTCTGTATTTATTATCGCCTTCATCGCAGCCCCTCCAGTAGATAT 5 0.125 No Hit TATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAA 5 0.125 No Hit CTGCGACTGCTGTTTTCTTGATTTACCCTATTGGTCAAGGAAGCTTCTCT 5 0.125 No Hit ATCGCCTTCATCGCAGCCCCTCCAGTAGATATTGATGGTATTCGCGAGCC 5 0.125 No Hit CTTATGAGCTAATTGTTCTACACTTCTTACTTGGTGTAGCTTGTTATATG 5 0.125 No Hit TATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAAT 5 0.125 No Hit GCGACTGCTGTTTTCTTGATTTACCCTATTGGTCAAGGAAGCTTCTCTGA 5 0.125 No Hit TGAAAATGAATCTGCTAATGAGGGTTACAAATTTGGTCAAGAGGAAGAGA 5 0.125 No Hit ATTTGGTCAAGAGGAAGAGACTTATAATATTGTGGCTGCTCATGGTTATT 5 0.125 No Hit ATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAA 5 0.125 No Hit GCTGTTTTCTTGATTTACCCTATTGGTCAAGGAAGCTTCTCTGATGGTAT 5 0.125 No Hit GGTTATACAATGGTGGTCCTTATGAGCTAATTGTTCTACACTTCTTACTT 5 0.125 No Hit ATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCTAAATGG 5 0.125 No Hit GTTGATAGTCAAGGTCGCGTTATTAATACTTGGGCTGATATCATCAACCG 5 0.125 No Hit TCTAGTTTGATCAGGGAAACTACTGAAAATGAATCTGCTAATGAGGGTTA 5 0.125 No Hit CTTAGTTTCCGTTTGGGTATGCGCCCTTGGATTGCTGTTGCATATTCAGC 5 0.125 No Hit GGAATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCTAAA 5 0.125 No Hit ATTCAGTGCTATGCATGGTTCCTTGGTAACCTCTAGTTTGATCAGGGAAA 5 0.125 No Hit CAGGATGAACGCTGGCGGCATGCTTAACACATGCAAGTCGAACGGGAAGT 5 0.125 No Hit AGAACCTTACCAGGGCTTGACATGCCGCGAATCCTCTTGAAAGAGAGGGG 5 0.125 No Hit AGCTTGTTATATGGGTCGTGAGTGGGAACTTAGTTTCCGTTTGGGTATGC 5 0.125 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0125 0.0 0.0 0.0 0.0 58-59 0.025 0.0 0.0 0.0 0.0 60-61 0.025 0.0 0.0 0.0 0.0 62-63 0.05 0.0 0.0 0.0 0.0 64-65 0.05 0.0 0.0 0.0 0.0 66-67 0.05 0.0 0.0 0.0 0.0 68-69 0.0625 0.0 0.0 0.0 0.0 70-71 0.1375 0.0 0.0 0.0 0.0 72-73 0.2875 0.0 0.0 0.0 0.0 74-75 0.3875 0.0 0.0 0.0 0.0 76-77 0.4375 0.0 0.0 0.0 0.0 78-79 0.525 0.0 0.0 0.0 0.0 80-81 0.625 0.0 0.0 0.0 0.0 82-83 0.7625 0.0 0.0 0.0 0.0 84-85 0.8875 0.0 0.0 0.0 0.0 86-87 1.05 0.0 0.0 0.0 0.0 88-89 1.225 0.0 0.0 0.0 0.0 90-91 1.4 0.0 0.0 0.0 0.0 92-93 1.65 0.0 0.0 0.0 0.0 94-95 2.0125 0.0 0.0 0.0 0.0 96-97 2.1500000000000004 0.0 0.0 0.0 0.0 98-99 2.4125 0.0 0.0 0.0 0.0 100-101 2.7375 0.0 0.0 0.0 0.0 102-103 3.0875000000000004 0.0 0.0 0.0 0.0 104-105 3.525 0.0 0.0 0.0 0.0 106-107 3.9000000000000004 0.0 0.0 0.0 0.0 108-109 3.975 0.0 0.0 0.0 0.0 110-111 3.975 0.0 0.0 0.0 0.0 112-113 3.975 0.0 0.0 0.0 0.0 114-115 3.975 0.0 0.0 0.0 0.0 116-117 3.975 0.0 0.0 0.0 0.0 118-119 3.975 0.0 0.0 0.0 0.0 120-121 3.975 0.0 0.0 0.0 0.0 122-123 3.975 0.0 0.0 0.0 0.0 124-125 3.975 0.0 0.0 0.0 0.0 126-127 3.975 0.0 0.0 0.0 0.0 128-129 3.975 0.0 0.0 0.0 0.0 130-131 3.975 0.0 0.0 0.0 0.0 132-133 3.975 0.0 0.0 0.0 0.0 134-135 3.975 0.0 0.0 0.0 0.0 136-137 3.975 0.0 0.0 0.0 0.0 138-139 3.975 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content pass >>END_MODULE Read 1333385 spots for SRR6941558.sra Written 1333385 spots for SRR6941558.sra Read 1333385 spots for SRR6941558.sra Written 1333385 spots for SRR6941558.sra Read 1333385 spots for SRR6941558.sra Written 1333385 spots for SRR6941558.sra Read 1333401 spots for SRR6941558.sra Written 1333401 spots for SRR6941558.sra Read 1333385 spots for SRR6941558.sra Written 1333385 spots for SRR6941558.sra Read 1333385 spots for SRR6941558.sra Written 1333385 spots for SRR6941558.sra Read 1333385 spots for SRR6941558.sra Written 1333385 spots for SRR6941558.sra Read 1333385 spots for SRR6941558.sra Written 1333385 spots for SRR6941558.sra Read 1333385 spots for SRR6941558.sra Written 1333385 spots for SRR6941558.sra Read 1333385 spots for SRR6941558.sra Written 1333385 spots for SRR6941558.sra Read 1333385 spots for SRR6941558.sra Written 1333385 spots for SRR6941558.sra Read 1333385 spots for SRR6941558.sra Written 1333385 spots for SRR6941558.sra Read 1333385 spots for SRR6941558.sra Written 1333385 spots for SRR6941558.sra Read 1333385 spots for SRR6941558.sra Written 1333385 spots for SRR6941558.sra Read 1333385 spots for SRR6941558.sra Written 1333385 spots for SRR6941558.sra Read 1333385 spots for SRR6941558.sra Written 1333385 spots for SRR6941558.sra Read 1333385 spots for SRR6941558.sra Written 1333385 spots for SRR6941558.sra Read 1333385 spots for SRR6941558.sra Written 1333385 spots for SRR6941558.sra Read 1333385 spots for SRR6941558.sra Written 1333385 spots for SRR6941558.sra Read 1333385 spots for SRR6941558.sra Written 1333385 spots for SRR6941558.sra SRR ids: ['SRR6941558.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_5erekmdr SRR6941558.sra spots: 26667716 blocks: [[1, 1333385], [1333386, 2666770], [2666771, 4000155], [4000156, 5333540], [5333541, 6666925], [6666926, 8000310], [8000311, 9333695], [9333696, 10667080], [10667081, 12000465], [12000466, 13333850], [13333851, 14667235], [14667236, 16000620], [16000621, 17334005], [17334006, 18667390], [18667391, 20000775], [20000776, 21334160], [21334161, 22667545], [22667546, 24000930], [24000931, 25334315], [25334316, 26667716]] SRR6941558 file size 9015113 SRR6941558 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR6941558 SRR6941558_1.fastq SRR6941558_2.fastq Input file: SRR6941558_1.fastq Paired file: SRR6941558_2.fastq trimmed: SRR6941558-trimmed-pair1.fastq, SRR6941558-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Fri Dec 6 11:20:09 2024 >> started Fri Dec 6 11:20:39 2024 >> done (30.177s) 26667716 read pairs processed; of these: 30809 ( 0.12%) short read pairs filtered out after trimming by size control 29862 ( 0.11%) empty read pairs filtered out after trimming by size control 26607045 (99.77%) read pairs available; of these: 21839862 (82.08%) trimmed read pairs available after processing 4767183 (17.92%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 1 0.00% 19 1 0.00% 20 1 0.00% 21 1 0.00% 22 4 0.00% 23 2 0.00% 24 4 0.00% 25 5 0.00% 26 15 0.00% 27 18 0.00% 28 13 0.00% 29 13 0.00% 30 15 0.00% 31 26 0.00% 32 30 0.00% 33 32 0.00% 34 41 0.00% 35 53 0.00% 36 54 0.00% 37 69 0.00% 38 92 0.00% 39 100 0.00% 40 141 0.00% 41 146 0.00% 42 181 0.00% 43 217 0.00% 44 234 0.00% 45 308 0.00% 46 341 0.00% 47 367 0.00% 48 451 0.00% 49 559 0.00% 50 643 0.00% 51 757 0.00% 52 824 0.00% 53 918 0.00% 54 1096 0.00% 55 1362 0.01% 56 1504 0.01% 57 1817 0.01% 58 1956 0.01% 59 2035 0.01% 60 2535 0.01% 61 2988 0.01% 62 3591 0.01% 63 3950 0.01% 64 4453 0.02% 65 4945 0.02% 66 5608 0.02% 67 5754 0.02% 68 6835 0.03% 69 8050 0.03% 70 8959 0.03% 71 10312 0.04% 72 11894 0.04% 73 13205 0.05% 74 13186 0.05% 75 15049 0.06% 76 14614 0.05% 77 17346 0.07% 78 17285 0.06% 79 18731 0.07% 80 20522 0.08% 81 21947 0.08% 82 23929 0.09% 83 25273 0.09% 84 26805 0.10% 85 31544 0.12% 86 31987 0.12% 87 34454 0.13% 88 38104 0.14% 89 37975 0.14% 90 43290 0.16% 91 41113 0.15% 92 45985 0.17% 93 47504 0.18% 94 54829 0.21% 95 50990 0.19% 96 48513 0.18% 97 48112 0.18% 98 45894 0.17% 99 46129 0.17% 100 48188 0.18% 101 50876 0.19% 102 56475 0.21% 103 57782 0.22% 104 57901 0.22% 105 59916 0.23% 106 59092 0.22% 107 58625 0.22% 108 61813 0.23% 109 69323 0.26% 110 67063 0.25% 111 75097 0.28% 112 89376 0.34% 113 67301 0.25% 114 85886 0.32% 115 73433 0.28% 116 96294 0.36% 117 97340 0.37% 118 94177 0.35% 119 113371 0.43% 120 124155 0.47% 121 124801 0.47% 122 130484 0.49% 123 138832 0.52% 124 145294 0.55% 125 148858 0.56% 126 149832 0.56% 127 161640 0.61% 128 188412 0.71% 129 259376 0.97% 130 499157 1.88% 131 1031024 3.88% 132 763583 2.87% 133 4040165 15.18% 134 5126811 19.27% 135 80942 0.30% 136 65692 0.25% 137 66554 0.25% 138 80062 0.30% 139 114342 0.43% 140 530569 1.99% 141 1585061 5.96% 142 205993 0.77% 143 2030424 7.63% 144 107445 0.40% 145 615215 2.31% 146 39779 0.15% 147 46819 0.18% 148 59529 0.22% 149 91790 0.34% 150 677257 2.55% 151 4767183 17.92% 26607045 reads passed initial QC criterion=sequence-density sequence-density=0.43 sequence-density-rank=1 fanout-score=1.94 fanout-score-rank=31 prefix-density=0.43 prefix-fanout=1.9 sequence=CCAGCCTCACGC criterion=fanout-score sequence-density=0.01 sequence-density-rank=33 fanout-score=310.52 fanout-score-rank=1 prefix-density=2.08 prefix-fanout=1.0 sequence=GTAGAACAAGATATTGGGTATTTCTCGCTTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCATTAATAGCGGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTACGTTCGTGCATTACTTCCATACCAAGATTAGCACGGTTGATGATATCAGCCCAAGTATTAATAACGCGACCTTGACTATCAACTACAGATTGGTTGAAATTGAATCCATTTAGGTTGAACGCCATAGTACTAATACCTAAAGCAGTGAACCAGATTCCTACTACAGGCCAAGCAGCCAAGAAGAAGTGTAAAGAACGAGAGTTGTTGAAACTAGCATATTGGAAGATTAATCGGCCAAAATAACCATGAGCAGCCACAATATTATAAGTCTCTTCCTCTTGACCAAATTTGTAACCCTCATTAGCAGATTCATTTTCAGTAGTTTCCCTGATCAAACTAGAGGTTACCAAGGAACCATGCATAGCACTGAATAGGGAACCG criterion=sequence-density sequence-density=0.20 sequence-density-rank=1 fanout-score=2.05 fanout-score-rank=25 prefix-density=0.20 prefix-fanout=2.0 sequence=CATTGGGTATATT criterion=fanout-score sequence-density=0.04 sequence-density-rank=30 fanout-score=37.52 fanout-score-rank=1 prefix-density=1.57 prefix-fanout=1.1 sequence=TGGTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGTTAACGAACGAGACCTCAGCCTGCTAACTAGCTATGCGGAGCCATCCCTCCGCAGCTAGCTTCTTAGAGGGACTATCGCCGTTTAGGCGACGGAAGTTTGAGGCAATAACAGGTCTGTGATGCCCTTAGATGTTCTGGGCCGCACGCGCGCTACACTGATGTATTCAACGAGTATATAGCCTTGGCCGACAGGCCCGGGTAATCTTGGGAAATTTCATCGTGATGGGGATAGATCATTGCAATTGTTGGTCTTCAACGAGGAATGCCTAGTAAGCGCGAGTCATCAGCTCGCGTTGACTACGTCCCTGCCCTTTGTACACACC SRR6941558 testing PE reads STAR mapping to Ensembl genome Started job on | Dec 06 11:21:13 Started mapping on | Dec 06 11:21:13 Finished on | Dec 06 11:23:08 Mapping speed, Million of reads per hour | 832.92 Number of input reads | 26607045 Average input read length | 271 UNIQUE READS: Uniquely mapped reads number | 14206788 Uniquely mapped reads % | 53.39% Average mapped length | 272.80 Number of splices: Total | 2700885 Number of splices: Annotated (sjdb) | 2345425 Number of splices: GT/AG | 2473779 Number of splices: GC/AG | 31643 Number of splices: AT/AC | 10622 Number of splices: Non-canonical | 184841 Mismatch rate per base, % | 0.32% Deletion rate per base | 0.01% Deletion average length | 1.48 Insertion rate per base | 0.01% Insertion average length | 1.93 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 10106146 % of reads mapped to multiple loci | 37.98% Number of reads mapped to too many loci | 92926 % of reads mapped to too many loci | 0.35% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 6.76% % of reads unmapped: other | 1.51% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 2314011 2314011 2314011 N_multimapping 10106146 10106146 10106146 N_noFeature 7011606 13804386 7181432 N_ambiguous 482165 7708 254619 UnstrandedReadsAssigned:6713017 PositiveStrandReadsAssigned:394694 NegativeStrandReadsAssigned:6770737 Dataset is classified negative stranded MeadianReadLen=151 20thPercentileLength=150 echo kmer=145 SRR6941558 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,972 [index] number of k-mers: 66,720,672 [index] number of equivalence classes: 111,837 [quant] running in paired-end mode [quant] will process pair 1: SRR6941558-trimmed-pair1.fastq SRR6941558-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 26,607,045 reads, 13,716,910 reads pseudoaligned [quant] estimated average fragment length: 205.981 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,078 rounds 52973 SRR6941558.ke.tsv 35125 SRR6941558.se.tsv 88098 total ==> SRR6941558.ke.tsv <== target_id length eff_length est_counts tpm PNS24245 936 731.469 0 0 PNS24247 1044 839.019 4.79787 0.331004 PNS24249 1928 1723.02 15.0811 0.506641 PNS24246 1044 839.019 4.79787 0.331004 PNS24248 1044 839.019 4.79787 0.331004 PNS24244 1471 1266.02 21.5253 0.984159 PNS24243 293 111.699 0 0 KQK14069 1603 1398.02 1232.41 51.0267 KQK14071 474 272.982 28.9334 6.13509 ==> SRR6941558.se.tsv <== BRADI_1g14170v3 1402 BRADI_1g53295v3 13 BRADI_1g59795v3 19 BRADI_1g07683v3 0 BRADI_1g00485v3 0 BRADI_1g20270v3 36 BRADI_1g74790v3 20 BRADI_1g09890v3 0 BRADI_1g77505v3 16 BRADI_1g48960v3 0 SRR6941558 completed mapping pipeline successfully