Starting /dee2/code/volunteer_pipeline.sh SRR6941560 current disk space = 1551240007680 free memory = 1600231764 SRR6941560 SRAfilesize 21fe65eb3a8b7b67820c0848a704dd4d SRR6941560.sra SRR6941560.sra file validated SRR6941560 is paired end SRR6941560 is conventional basespace SRR6941560 read1 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR6941560_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 34.7315 35.0 35.0 35.0 35.0 35.0 2 34.67525 35.0 35.0 35.0 35.0 35.0 3 34.72225 35.0 35.0 35.0 35.0 35.0 4 34.7315 35.0 35.0 35.0 35.0 35.0 5 34.63325 35.0 35.0 35.0 35.0 35.0 6 39.558 40.0 40.0 40.0 39.0 40.0 7 39.4815 40.0 40.0 40.0 39.0 40.0 8 39.58125 40.0 40.0 40.0 39.0 40.0 9 39.54775 40.0 40.0 40.0 39.0 40.0 10-14 39.55215 40.0 40.0 40.0 39.0 40.0 15-19 39.505 40.0 40.0 40.0 39.0 40.0 20-24 39.507349999999995 40.0 40.0 40.0 39.0 40.0 25-29 39.4833 40.0 40.0 40.0 39.0 40.0 30-34 39.32225 40.0 40.0 40.0 39.0 40.0 35-39 39.48225000000001 40.0 40.0 40.0 39.0 40.0 40-44 39.44565 40.0 40.0 40.0 39.0 40.0 45-49 39.418549999999996 40.0 40.0 40.0 39.0 40.0 50-54 39.3792 40.0 40.0 40.0 39.0 40.0 55-59 39.31515 40.0 40.0 40.0 39.0 40.0 60-64 39.3868 40.0 40.0 40.0 39.0 40.0 65-69 39.34215 40.0 40.0 40.0 39.0 40.0 70-74 39.2299 40.0 40.0 40.0 38.8 40.0 75-79 39.162400000000005 40.0 40.0 40.0 38.8 40.0 80-84 39.24865 40.0 40.0 40.0 39.0 40.0 85-89 39.2041 40.0 40.0 40.0 39.0 40.0 90-94 39.07875 40.0 39.8 40.0 38.6 40.0 95-99 39.1342 40.0 40.0 40.0 38.6 40.0 100-104 38.294 39.2 38.6 39.6 36.8 39.8 105-109 39.174400000000006 40.0 39.8 40.0 38.8 40.0 110-114 39.224450000000004 40.0 40.0 40.0 39.0 40.0 115-119 39.284299999999995 40.0 40.0 40.0 39.0 40.0 120-124 39.04615 40.0 39.8 40.0 38.2 40.0 125-129 38.9869 40.0 39.4 40.0 38.0 40.0 130-134 38.9798 40.0 39.2 40.0 37.8 40.0 135-139 38.91065 40.0 39.0 40.0 37.6 40.0 140-144 38.662400000000005 40.0 39.0 40.0 36.6 40.0 145-149 38.79445 40.0 39.0 40.0 37.6 40.0 150-151 37.13775 39.5 37.5 40.0 33.5 40.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 11 1.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 2.0 25 2.0 26 5.0 27 9.0 28 8.0 29 16.0 30 20.0 31 18.0 32 41.0 33 33.0 34 38.0 35 65.0 36 92.0 37 122.0 38 253.0 39 3275.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 47.406664996241545 11.250313204710599 6.364319719368579 34.97870207967928 2 23.549999999999997 13.5 32.2 30.75 3 21.25 19.15 26.25 33.35 4 25.074999999999996 26.55 22.175 26.200000000000003 5 24.32296890672016 32.19658976930793 23.244734202607823 20.23570712136409 6 20.525 36.925000000000004 23.35 19.2 7 13.275 31.724999999999998 38.7 16.3 8 17.0 28.449999999999996 32.125 22.425 9 16.375 25.775 36.85 21.0 10-14 18.52 34.575 25.685000000000002 21.22 15-19 19.605 31.635 25.990000000000002 22.770000000000003 20-24 18.80876175235047 31.791358271654328 27.830566113222645 21.569313862772553 25-29 21.722172217221722 31.233123312331234 26.932693269326936 20.11201120112011 30-34 21.75108755437772 32.58662933146657 25.031251562578127 20.63103155157758 35-39 20.45 30.990000000000002 27.584999999999997 20.974999999999998 40-44 18.375 31.195 27.400000000000002 23.03 45-49 19.0 31.035 28.544999999999998 21.42 50-54 20.465 31.069999999999997 26.86 21.605 55-59 20.615 30.785 25.540000000000003 23.06 60-64 19.045 31.430000000000003 27.27 22.255 65-69 19.689999999999998 31.7 26.355 22.255 70-74 19.495 31.314999999999998 25.224999999999998 23.965 75-79 20.169999999999998 30.42 26.955000000000002 22.455 80-84 22.06 29.865000000000002 26.305 21.77 85-89 20.935000000000002 30.154999999999998 26.515 22.395 90-94 18.935 31.255 27.02 22.79 95-99 19.994999999999997 31.71 24.37 23.925 100-104 20.23 32.879999999999995 25.224999999999998 21.665 105-109 19.535 31.405 26.405 22.655 110-114 20.26 30.154999999999998 26.655 22.93 115-119 19.62 32.005 24.735 23.64 120-124 19.812831548393554 31.598438594735264 24.256831148033232 24.331898708837954 125-129 19.483896779355874 32.21144228845769 24.844968993798762 23.459691938387678 130-134 21.4 32.21 23.955000000000002 22.435 135-139 21.765 31.009999999999998 25.09 22.134999999999998 140-144 22.06 30.625000000000004 25.759999999999998 21.555 145-149 20.13 31.205 25.330000000000002 23.335 150-151 19.80706589827111 32.69857178651967 24.05412177399148 23.44024054121774 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.5 3 0.5 4 0.0 5 0.0 6 0.5 7 0.5 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 1.0 20 2.5 21 3.5 22 6.5 23 6.0 24 8.0 25 11.0 26 12.0 27 13.0 28 18.5 29 26.5 30 25.0 31 28.0 32 35.5 33 37.5 34 48.5 35 55.0 36 86.5 37 201.5 38 281.5 39 272.5 40 293.5 41 313.0 42 259.5 43 260.5 44 280.0 45 242.0 46 185.0 47 151.5 48 135.5 49 94.5 50 67.5 51 63.5 52 52.5 53 38.0 54 46.0 55 57.0 56 52.0 57 35.5 58 31.5 59 35.5 60 33.5 61 23.0 62 13.0 63 11.0 64 14.5 65 12.0 66 5.5 67 2.5 68 3.5 69 2.5 70 0.5 71 0.5 72 1.0 73 1.0 74 0.0 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.22499999999999998 2 0.0 3 0.0 4 0.0 5 0.3 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.02 25-29 0.01 30-34 0.005 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.09 125-129 0.02 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.22499999999999998 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 64.125 #Duplication Level Percentage of deduplicated Percentage of total 1 84.83430799220272 54.400000000000006 2 7.251461988304094 9.3 3 2.807017543859649 5.4 4 1.4035087719298245 3.5999999999999996 5 0.5847953216374269 1.875 6 0.7407407407407408 2.85 7 0.3898635477582846 1.7500000000000002 8 0.31189083820662766 1.6 9 0.31189083820662766 1.7999999999999998 >10 1.3255360623781676 15.775 >50 0.03898635477582846 1.6500000000000001 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GCGGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTACG 66 1.6500000000000001 No Hit CTTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCAT 43 1.075 No Hit GGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTACGTT 40 1.0 No Hit GGCCAAAATAACCATGAGCAGCCACAATATTATAAGTCTCTTCCTCTTGA 33 0.8250000000000001 No Hit GCTTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCA 32 0.8 No Hit CTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCATTAAT 28 0.7000000000000001 No Hit GTTAGCGGAAAAACCTTATCCATTAATAGCGGGAACTTCAAGAGCAGCTA 28 0.7000000000000001 No Hit CTCATAAGGACCACCATTGTATAACCATTCATCAACGGATGCAGCTTCCC 23 0.575 No Hit GTGCTCTGCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTCCTA 22 0.5499999999999999 No Hit GCGGAAAAACCTTATCCATTAATAGCGGGAACTTCAAGAGCAGCTAGATC 21 0.525 No Hit GCTCTGCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTCCTAAA 21 0.525 No Hit GCGACCTTGACTATCAACTACAGATTGGTTGAAATTGAATCCATTTAGGT 19 0.475 No Hit AGCGGAAAAACCTTATCCATTAATAGCGGGAACTTCAAGAGCAGCTAGAT 19 0.475 No Hit GTGAGCATTACGTTCGTGCATTACTTCCATACCAAGATTAGCACGGTTGA 18 0.44999999999999996 No Hit GCCAAAATAACCATGAGCAGCCACAATATTATAAGTCTCTTCCTCTTGAC 18 0.44999999999999996 No Hit CTCTGCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTCCTAAAG 18 0.44999999999999996 No Hit CCCAAGTATTAATAACGCGACCTTGACTATCAACTACAGATTGGTTGAAA 17 0.42500000000000004 No Hit CGCGACCTTGACTATCAACTACAGATTGGTTGAAATTGAATCCATTTAGG 17 0.42500000000000004 No Hit GCACGGTTGATGATATCAGCCCAAGTATTAATAACGCGACCTTGACTATC 16 0.4 No Hit GGATGCATAAGGATGTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAA 16 0.4 No Hit GGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTACGTTC 16 0.4 No Hit CGGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTACGT 15 0.375 No Hit GTTGAAACTAGCATATTGGAAGATTAATCGGCCAAAATAACCATGAGCAG 15 0.375 No Hit GTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTC 14 0.35000000000000003 No Hit GGTCAATAAGGTAGGGATCATCAAAACACCGAACCATCCGATGTAAAGAC 13 0.325 No Hit CCACCATTGTATAACCATTCATCAACGGATGCAGCTTCCCAAATTGGGTA 12 0.3 No Hit GTTGATGATATCAGCCCAAGTATTAATAACGCGACCTTGACTATCAACTA 12 0.3 No Hit GGCCAAGCAGCCAAGAAGAAGTGTAAAGAACGAGAGTTGTTGAAACTAGC 12 0.3 No Hit CCACAATATTATAAGTCTCTTCCTCTTGACCAAATTTGTAACCCTCATTA 11 0.27499999999999997 No Hit GCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTCCTAAAGGCAT 11 0.27499999999999997 No Hit GCCAAGAAGAAGTGTAAAGAACGAGAGTTGTTGAAACTAGCATATTGGAA 11 0.27499999999999997 No Hit GGAGGGGCTGCGATGAAGGCGATAATAAATACAGAAGTTGCGGTCAATAA 10 0.25 No Hit CATATAACAAGCTACACCAAGTAAGAAGTGTAGAACAATTAGCTCATAAG 10 0.25 No Hit GCTGCGATGAAGGCGATAATAAATACAGAAGTTGCGGTCAATAAGGTAGG 10 0.25 No Hit GACCCATATAACAAGCTACACCAAGTAAGAAGTGTAGAACAATTAGCTCA 10 0.25 No Hit CGCCATAGTACTAATACCTAAAGCAGTGAACCAGATTCCTACTACAGGCC 9 0.22499999999999998 No Hit CGCAGAAGTAGGAATAATAGCACCAGAGATAATATTGTTTCCATAAAGTA 9 0.22499999999999998 No Hit CCGCAGAAGTAGGAATAATAGCACCAGAGATAATATTGTTTCCATAAAGT 9 0.22499999999999998 No Hit GGCGATAATAAATACAGAAGTTGCGGTCAATAAGGTAGGGATCATCAAAA 9 0.22499999999999998 No Hit GGGAAGTTGTGAGCATTACGTTCGTGCATTACTTCCATACCAAGATTAGC 9 0.22499999999999998 No Hit GTTGAAATTGAATCCATTTAGGTTGAACGCCATAGTACTAATACCTAAAG 9 0.22499999999999998 No Hit CTCATTAGCAGATTCATTTTCAGTAGTTTCCCTGATCAAACTAGAGGTTA 9 0.22499999999999998 No Hit GCCCAAGTATTAATAACGCGACCTTGACTATCAACTACAGATTGGTTGAA 9 0.22499999999999998 No Hit GGTTGATGATATCAGCCCAAGTATTAATAACGCGACCTTGACTATCAACT 8 0.2 No Hit GGCGCATACCCAAACGGAAACTAAGTTCCCACTCACGACCCATATAACAA 8 0.2 No Hit TTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCATTAATA 8 0.2 No Hit ACGCGACCTTGACTATCAACTACAGATTGGTTGAAATTGAATCCATTTAG 8 0.2 No Hit CATCAATATCTACTGGAGGGGCTGCGATGAAGGCGATAATAAATACAGAA 8 0.2 No Hit CTCTTGACCAAATTTGTAACCCTCATTAGCAGATTCATTTTCAGTAGTTT 8 0.2 No Hit CCTCATTAGCAGATTCATTTTCAGTAGTTTCCCTGATCAAACTAGAGGTT 8 0.2 No Hit CTCGCGAATACCATCAATATCTACTGGAGGGGCTGCGATGAAGGCGATAA 8 0.2 No Hit GGCGGAAAAACCTTATCCATTAATAGCGGGAACTTCAAGAGCAGCTAGAT 7 0.17500000000000002 No Hit GTCTCTTCCTCTTGACCAAATTTGTAACCCTCATTAGCAGATTCATTTTC 7 0.17500000000000002 No Hit TTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCATT 7 0.17500000000000002 No Hit TTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCATTA 7 0.17500000000000002 No Hit CCCTCATTAGCAGATTCATTTTCAGTAGTTTCCCTGATCAAACTAGAGGT 7 0.17500000000000002 No Hit CGGAAAAACCTTATCCATTAATAGCGGGAACTTCAAGAGCAGCTAGATCT 7 0.17500000000000002 No Hit GTAGGAATAATAGCACCAGAGATAATATTGTTTCCATAAAGTAAAGAACC 7 0.17500000000000002 No Hit GGTAAATCAAGAAAACAGCAGTCGCAGCTGCAACAGGAGCTGAATATGCA 7 0.17500000000000002 No Hit CTTATCCATTAATAGCGGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAG 7 0.17500000000000002 No Hit GGGGCTGCGATGAAGGCGATAATAAATACAGAAGTTGCGGTCAATAAGGT 7 0.17500000000000002 No Hit GCGGTCAATAAGGTAGGGATCATCAAAACACCGAACCATCCGATGTAAAG 6 0.15 No Hit CCCATATAACAAGCTACACCAAGTAAGAAGTGTAGAACAATTAGCTCATA 6 0.15 No Hit GTGTAAAGAACGAGAGTTGTTGAAACTAGCATATTGGAAGATTAATCGGC 6 0.15 No Hit GGAAGATTAATCGGCCAAAATAACCATGAGCAGCCACAATATTATAAGTC 6 0.15 No Hit CTGCGATGAAGGCGATAATAAATACAGAAGTTGCGGTCAATAAGGTAGGG 6 0.15 No Hit CTTGACTATCAACTACAGATTGGTTGAAATTGAATCCATTTAGGTTGAAC 6 0.15 No Hit GGAAAAACCTTATCCATTAATAGCGGGAACTTCAAGAGCAGCTAGATCTA 6 0.15 No Hit ACCAGATATTCCTAAAGGCATACCATCAGAGAAGCTTCCTTGACCAATAG 6 0.15 No Hit CCCTCTTCAAATAGATCTAATGGATAAGCTACATAACAGATCCATTGACT 6 0.15 No Hit GCTTAATAGTACATCCCAATAAAGGACGACCATACTTGTTCAACTTATCT 6 0.15 No Hit ATCCGATTGCCGCAGAAGTAGGAATAATAGCACCAGAGATAATATTGTTT 6 0.15 No Hit CCATATAACAAGCTACACCAAGTAAGAAGTGTAGAACAATTAGCTCATAA 6 0.15 No Hit GCACTGAATAGGGAACCGCCGAAAACACCAGCTACACCTAACATGTGAAA 6 0.15 No Hit AGGAATAATAGCACCAGAGATAATATTGTTTCCATAAAGTAAAGAACCAG 6 0.15 No Hit GTGCTAGTTATCCAGTTGCAGAAGCGACCCCACAGGCTTGTACTTTCGCG 6 0.15 No Hit ATCAATATCTACTGGAGGGGCTGCGATGAAGGCGATAATAAATACAGAAG 6 0.15 No Hit GATGCATAAGGATGTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAAA 6 0.15 No Hit CCCAGGAACAGGCTCGATGTGATAGCATCGTCCTTTGTAACGATCAAGAC 6 0.15 No Hit CACCATTGTATAACCATTCATCAACGGATGCAGCTTCCCAAATTGGGTAA 6 0.15 No Hit ACCATGAGCAGCCACAATATTATAAGTCTCTTCCTCTTGACCAAATTTGT 5 0.125 No Hit CAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCATTAATAGCGGGAA 5 0.125 No Hit GCCGAAAACACCAGCTACACCTAACATGTGAAATGGATGCATAAGGATGT 5 0.125 No Hit GGCCACCATTGTATAACCATTCATCAACGGATGCAGCTTCCCAAATTGGG 5 0.125 No Hit CGAGAGTTGTTGAAACTAGCATATTGGAAGATTAATCGGCCAAAATAACC 5 0.125 No Hit CAGTGAACCAGATTCCTACTACAGGCCAAGCAGCCAAGAAGAAGTGTAAA 5 0.125 No Hit GCCATAGTACTAATACCTAAAGCAGTGAACCAGATTCCTACTACAGGCCA 5 0.125 No Hit ATAAGGATGTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAAAGTACC 5 0.125 No Hit GATGATATCAGCCCAAGTATTAATAACGCGACCTTGACTATCAACTACAG 5 0.125 No Hit GCATATTGGAAGATTAATCGGCCAAAATAACCATGAGCAGCCACAATATT 5 0.125 No Hit ACCTTATCCATTAATAGCGGGAACTTCAAGAGCAGCTAGATCTAGAGGGA 5 0.125 No Hit CTCGCTTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTAT 5 0.125 No Hit CTTGACCAAATTTGTAACCCTCATTAGCAGATTCATTTTCAGTAGTTTCC 5 0.125 No Hit GCATAAGGATGTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAAAGTA 5 0.125 No Hit CCATGAGCAGCCACAATATTATAAGTCTCTTCCTCTTGACCAAATTTGTA 5 0.125 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.025 0.0 0.0 0.0 0.0 62-63 0.025 0.0 0.0 0.0 0.0 64-65 0.05 0.0 0.0 0.0 0.0 66-67 0.05 0.0 0.0 0.0 0.0 68-69 0.05 0.0 0.0 0.0 0.0 70-71 0.075 0.0 0.0 0.0 0.0 72-73 0.075 0.0 0.0 0.0 0.0 74-75 0.075 0.0 0.0 0.0 0.0 76-77 0.075 0.0 0.0 0.0 0.0 78-79 0.075 0.0 0.0 0.0 0.0 80-81 0.1 0.0 0.0 0.0 0.0 82-83 0.125 0.0 0.0 0.0 0.0 84-85 0.1875 0.0 0.0 0.0 0.0 86-87 0.3375 0.0 0.0 0.0 0.0 88-89 0.42500000000000004 0.0 0.0 0.0 0.0 90-91 0.5625 0.0 0.0 0.0 0.0 92-93 0.7875 0.0 0.0 0.0 0.0 94-95 0.925 0.0 0.0 0.0 0.0 96-97 1.0375 0.0 0.0 0.0 0.0 98-99 1.125 0.0 0.0 0.0 0.0 100-101 1.3 0.0 0.0 0.0 0.0 102-103 1.5375 0.0 0.0 0.0 0.0 104-105 1.7625 0.0 0.0 0.0 0.0 106-107 1.95 0.0 0.0 0.0 0.0 108-109 2.1500000000000004 0.0 0.0 0.0 0.0 110-111 2.325 0.0 0.0 0.0 0.0 112-113 2.6125 0.0 0.0 0.0 0.0 114-115 2.8625 0.0 0.0 0.0 0.0 116-117 3.175 0.0 0.0 0.0 0.0 118-119 3.4749999999999996 0.0 0.0 0.0 0.0 120-121 3.825 0.0 0.0 0.0 0.0 122-123 4.1875 0.0 0.0 0.0 0.0 124-125 4.6625 0.0 0.0 0.0 0.0 126-127 5.0 0.0 0.0 0.0 0.0 128-129 5.2875 0.0 0.0 0.0 0.0 130-131 5.65 0.0 0.0 0.0 0.0 132-133 6.025 0.0 0.0 0.0 0.0 134-135 6.4875 0.0 0.0 0.0 0.0 136-137 6.95 0.0 0.0 0.0 0.0 138-139 7.300000000000001 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCTTTCT 10 0.006830828 145.0 1 GAAGTTG 100 7.9452863E-4 11.599999 30-34 GCTAGAT 90 0.0048656333 11.277777 15-19 ATCTAGA 90 0.0048656333 11.277777 20-24 AGCTAGA 90 0.0048656333 11.277777 15-19 AGAGGGA 90 0.0048656333 11.277777 25-29 AGAGCAG 90 0.0048656333 11.277777 10-14 GATCTAG 90 0.0048656333 11.277777 20-24 GAGGGAA 90 0.0048656333 11.277777 25-29 TGTGAGC 95 0.007278115 10.684211 35-39 AAGTTGT 95 0.007278115 10.684211 30-34 >>END_MODULE SRR6941560 read2 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR6941560_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 33.88925 35.0 35.0 35.0 33.0 35.0 2 34.16 35.0 35.0 35.0 33.0 35.0 3 34.39375 35.0 35.0 35.0 33.0 35.0 4 34.3035 35.0 35.0 35.0 33.0 35.0 5 34.427 35.0 35.0 35.0 34.0 35.0 6 39.0805 40.0 40.0 40.0 39.0 40.0 7 39.04975 40.0 40.0 40.0 39.0 40.0 8 39.14025 40.0 40.0 40.0 39.0 40.0 9 39.22125 40.0 40.0 40.0 39.0 40.0 10-14 39.1566 40.0 40.0 40.0 38.8 40.0 15-19 39.2278 40.0 40.0 40.0 39.0 40.0 20-24 39.2813 40.0 40.0 40.0 39.0 40.0 25-29 39.2229 40.0 40.0 40.0 39.0 40.0 30-34 39.178000000000004 40.0 40.0 40.0 39.0 40.0 35-39 39.0374 40.0 39.8 40.0 38.4 40.0 40-44 39.1207 40.0 40.0 40.0 38.8 40.0 45-49 39.04415 40.0 40.0 40.0 38.6 40.0 50-54 38.85189999999999 40.0 39.6 40.0 37.6 40.0 55-59 38.83035 40.0 39.4 40.0 37.8 40.0 60-64 38.8022 40.0 39.2 40.0 37.2 40.0 65-69 38.875699999999995 40.0 39.4 40.0 37.6 40.0 70-74 38.711850000000005 40.0 39.0 40.0 36.8 40.0 75-79 38.712900000000005 40.0 39.4 40.0 37.2 40.0 80-84 38.74505 40.0 39.0 40.0 37.4 40.0 85-89 38.851099999999995 40.0 39.0 40.0 37.8 40.0 90-94 38.80364999999999 40.0 39.0 40.0 37.4 40.0 95-99 38.58805 40.0 39.0 40.0 36.6 40.0 100-104 37.3216 38.6 37.8 39.2 33.8 39.4 105-109 38.60725 40.0 39.0 40.0 36.4 40.0 110-114 38.699 40.0 39.0 40.0 37.0 40.0 115-119 38.52374999999999 40.0 39.0 40.0 36.4 40.0 120-124 38.506499999999996 40.0 39.0 40.0 36.6 40.0 125-129 38.57845 40.0 39.0 40.0 36.6 40.0 130-134 38.434749999999994 40.0 39.0 40.0 36.0 40.0 135-139 38.368050000000004 40.0 39.0 40.0 36.0 40.0 140-144 38.0793 40.0 39.0 40.0 35.2 40.0 145-149 37.7204 40.0 38.8 40.0 34.4 40.0 150-151 34.765125 38.0 34.5 39.5 25.0 40.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 0.0 4 0.0 5 0.0 6 1.0 7 0.0 8 0.0 9 0.0 10 0.0 11 1.0 12 0.0 13 0.0 14 2.0 15 1.0 16 3.0 17 0.0 18 5.0 19 2.0 20 3.0 21 4.0 22 4.0 23 4.0 24 6.0 25 5.0 26 10.0 27 8.0 28 27.0 29 20.0 30 29.0 31 30.0 32 42.0 33 38.0 34 70.0 35 86.0 36 85.0 37 165.0 38 368.0 39 2978.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.39046653144016 21.196754563894523 12.068965517241379 26.343813387423936 2 23.1600100477267 26.048731474503896 36.071338859583015 14.719919618186387 3 19.45 25.3 38.3 16.950000000000003 4 22.625 30.45 26.150000000000002 20.775 5 23.75 31.15 29.025000000000002 16.075 6 19.8 35.325 27.750000000000004 17.125 7 17.125 21.45 43.6 17.825 8 21.775 24.0 31.974999999999998 22.25 9 21.975 20.974999999999998 36.1 20.95 10-14 22.15 26.939999999999998 31.78 19.13 15-19 22.465 25.665 31.724999999999998 20.145 20-24 23.244999999999997 25.509999999999998 32.245000000000005 19.0 25-29 23.150000000000002 25.795 31.64 19.415 30-34 23.9 24.759999999999998 32.285000000000004 19.055 35-39 23.805 25.19 31.879999999999995 19.125 40-44 22.805 26.229999999999997 31.045 19.919999999999998 45-49 22.48 26.72 31.095 19.705000000000002 50-54 22.650000000000002 25.919999999999998 32.129999999999995 19.3 55-59 22.13 26.939999999999998 29.965000000000003 20.965 60-64 21.990000000000002 26.009999999999998 31.495 20.505000000000003 65-69 22.34 26.095000000000002 31.245 20.32 70-74 22.73 25.77 31.655 19.845 75-79 23.56 25.275 31.34 19.825 80-84 23.255 24.925 32.525 19.295 85-89 23.79 26.215 30.285 19.71 90-94 22.945 25.595000000000002 30.930000000000003 20.53 95-99 23.055 25.055 31.355 20.535 100-104 22.830000000000002 26.1 31.240000000000002 19.830000000000002 105-109 24.265 24.945 31.064999999999998 19.725 110-114 23.26 26.045 31.130000000000003 19.564999999999998 115-119 23.66 25.71 31.185000000000002 19.445 120-124 22.86 26.645000000000003 29.909999999999997 20.585 125-129 23.335 26.490000000000002 30.36 19.814999999999998 130-134 23.31 26.5 30.28 19.91 135-139 24.005000000000003 26.150000000000002 30.855 18.990000000000002 140-144 23.57 26.495 31.085 18.85 145-149 23.23 26.765 31.014999999999997 18.990000000000002 150-151 23.226372524442215 25.770869892203557 31.8751566808724 19.127600902481827 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.5 8 0.5 9 0.0 10 0.5 11 0.5 12 0.0 13 0.0 14 0.0 15 0.0 16 0.5 17 0.5 18 0.5 19 0.5 20 1.0 21 3.5 22 3.5 23 4.0 24 6.5 25 8.5 26 12.0 27 16.0 28 18.0 29 25.0 30 37.5 31 42.0 32 39.0 33 53.5 34 81.5 35 99.0 36 117.5 37 174.0 38 234.0 39 243.0 40 263.5 41 282.5 42 240.0 43 241.5 44 280.0 45 236.0 46 202.5 47 173.5 48 119.0 49 85.0 50 73.5 51 72.5 52 53.5 53 45.5 54 52.5 55 64.0 56 50.5 57 37.0 58 38.0 59 33.5 60 29.5 61 26.0 62 22.5 63 13.5 64 7.5 65 8.0 66 6.5 67 4.5 68 4.0 69 3.5 70 2.5 71 1.5 72 1.0 73 0.5 74 0.5 75 0.5 76 0.0 77 0.5 78 0.5 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 1.4000000000000001 2 0.475 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.27499999999999997 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 66.625 #Duplication Level Percentage of deduplicated Percentage of total 1 82.4015009380863 54.900000000000006 2 8.517823639774859 11.35 3 3.3020637898686678 6.6000000000000005 4 1.801125703564728 4.8 5 0.8630393996247656 2.875 6 0.7504690431519699 3.0 7 0.5628517823639775 2.625 8 0.37523452157598497 2.0 9 0.33771106941838647 2.025 >10 1.0881801125703565 9.825000000000001 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source ATTATCTATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAAT 27 0.675 No Hit GTTATTTTGGCCGATTAATCTTCCAATATGCTAGTTTCAACAACTCTCGT 22 0.5499999999999999 No Hit ATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAA 20 0.5 No Hit GTTAGGTGTAGCTGGTGTTTTCGGCGGTTCCCTATTCAGTGCTATGCATG 18 0.44999999999999996 No Hit TATCTATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTT 18 0.44999999999999996 No Hit GCTAGTTTCAACAACTCTCGTTCTTTACACTTCTTCTTGGCTGCTTGGCC 17 0.42500000000000004 No Hit CTATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAA 16 0.4 No Hit GGAAGCTTCTCTGATGGTATGCCTTTAGGAATATCTGGTACTTTCAACTT 14 0.35000000000000003 No Hit ATCTATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTG 14 0.35000000000000003 No Hit GAGCACAACATCCTTATGCATCCATTTCACATGTTAGGTGTAGCTGGTGT 14 0.35000000000000003 No Hit GTTCCTTGGTAACCTCTAGTTTGATCAGGGAAACTACTGAAAATGAATCT 13 0.325 No Hit ATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCA 13 0.325 No Hit GTTTCAACAACTCTCGTTCTTTACACTTCTTCTTGGCTGCTTGGCCTGTA 13 0.325 No Hit GTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGATCTTACCATGAC 13 0.325 No Hit GCATGGTTCCTTGGTAACCTCTAGTTTGATCAGGGAAACTACTGAAAATG 12 0.3 No Hit CTGGTACTTTCAACTTTATGATTGTATTCCAGGCAGAGCACAACATCCTT 11 0.27499999999999997 No Hit GTCGTGAGTGGGAACTTAGTTTCCGTTTGGGTATGCGCCCTTGGATTGCT 11 0.27499999999999997 No Hit CAACTCTCGTTCTTTACACTTCTTCTTGGCTGCTTGGCCTGTAGTAGGAA 11 0.27499999999999997 No Hit GCTTGTTATATGGGTCGTGAGTGGGAACTTAGTTTCCGTTTGGGTATGCG 11 0.27499999999999997 No Hit GTTCGATCCTGGCTCAGGATGAACGCTGGCGGCATGCTTAACACATGCAA 11 0.27499999999999997 No Hit GTGGGAACTTAGTTTCCGTTTGGGTATGCGCCCTTGGATTGCTGTTGCAT 11 0.27499999999999997 No Hit AGGGAAACTACTGAAAATGAATCTGCTAATGAGGGTTACAAATTTGGTCA 11 0.27499999999999997 No Hit GCACAACATCCTTATGCATCCATTTCACATGTTAGGTGTAGCTGGTGTTT 11 0.27499999999999997 No Hit CTTCCAATATGCTAGTTTCAACAACTCTCGTTCTTTACACTTCTTCTTGG 11 0.27499999999999997 No Hit AGCACAACATCCTTATGCATCCATTTCACATGTTAGGTGTAGCTGGTGTT 10 0.25 No Hit GGAAACTACTGAAAATGAATCTGCTAATGAGGGTTACAAATTTGGTCAAG 10 0.25 No Hit GTTATACAATGGTGGTCCTTATGAGCTAATTGTTCTACACTTCTTACTTG 10 0.25 No Hit GTAGGAATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCT 10 0.25 No Hit CTGAAAATGAATCTGCTAATGAGGGTTACAAATTTGGTCAAGAGGAAGAG 10 0.25 No Hit CTTTTACCCAATTTGGGAAGCTGCATCCGTTGATGAATGGTTATACAATG 9 0.22499999999999998 No Hit TATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAA 9 0.22499999999999998 No Hit ATTTGGTCAAGAGGAAGAGACTTATAATATTGTGGCTGCTCATGGTTATT 9 0.22499999999999998 No Hit GTTTTGATGATCCCTACCTTATTGACCGCAACTTCTGTATTTATTATCGC 9 0.22499999999999998 No Hit GGAATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCTAAA 9 0.22499999999999998 No Hit GCTGCGACTGCTGTTTTCTTGATTTACCCTATTGGTCAAGGAAGCTTCTC 9 0.22499999999999998 No Hit GAAGAGACTTATAATATTGTGGCTGCTCATGGTTATTTTGGCCGATTAAT 9 0.22499999999999998 No Hit GTTCAACCTAAATGGATTCAATTTCAACCAATCTGTAGTTGATAGTCAAG 9 0.22499999999999998 No Hit CAACATCCTTATGCATCCATTTCACATGTTAGGTGTAGCTGGTGTTTTCG 9 0.22499999999999998 No Hit GGAAGAGACTTATAATATTGTGGCTGCTCATGGTTATTTTGGCCGATTAA 8 0.2 No Hit CATGGTTATTTTGGCCGATTAATCTTCCAATATGCTAGTTTCAACAACTC 8 0.2 No Hit GGTAACCTCTAGTTTGATCAGGGAAACTACTGAAAATGAATCTGCTAATG 8 0.2 No Hit CCTAAATGGATTCAATTTCAACCAATCTGTAGTTGATAGTCAAGGTCGCG 8 0.2 No Hit GGGAAACTACTGAAAATGAATCTGCTAATGAGGGTTACAAATTTGGTCAA 8 0.2 No Hit CATGACTGCAATTTTAGAGAGACGCGAAAGTACAAGCCTGTGGGGTCGCT 8 0.2 No Hit CTTAGTTTCCGTTTGGGTATGCGCCCTTGGATTGCTGTTGCATATTCAGC 8 0.2 No Hit GTTGCATATTCAGCTCCTGTTGCAGCTGCGACTGCTGTTTTCTTGATTTA 8 0.2 No Hit ATTCAGTGCTATGCATGGTTCCTTGGTAACCTCTAGTTTGATCAGGGAAA 8 0.2 No Hit TTGTATTCCAGGCAGAGCACAACATCCTTATGCATCCATTTCACATGTTA 8 0.2 No Hit CCTTGTTATTGTGAGAATTCTTAATTCAAGAGTTGTAAGGAGGGACTTAT 7 0.17500000000000002 No Hit CCAAGATCTTACCATGACTGCAATTTTAGAGAGACGCGAAAGTACAAGCC 7 0.17500000000000002 No Hit GAGACTTATAATATTGTGGCTGCTCATGGTTATTTTGGCCGATTAATCTT 7 0.17500000000000002 No Hit GACTTATAATATTGTGGCTGCTCATGGTTATTTTGGCCGATTAATCTTCC 7 0.17500000000000002 No Hit GTTACAAATTTGGTCAAGAGGAAGAGACTTATAATATTGTGGCTGCTCAT 7 0.17500000000000002 No Hit GCTGTTTTCTTGATTTACCCTATTGGTCAAGGAAGCTTCTCTGATGGTAT 7 0.17500000000000002 No Hit CTAGCACTGAAAATCGTCTTTACATCGGATGGTTCGGTGTTTTGATGATC 7 0.17500000000000002 No Hit CAAATTTGGTCAAGAGGAAGAGACTTATAATATTGTGGCTGCTCATGGTT 7 0.17500000000000002 No Hit GTAACCTCTAGTTTGATCAGGGAAACTACTGAAAATGAATCTGCTAATGA 7 0.17500000000000002 No Hit CGCGAAAGTACAAGCCTGTGGGGTCGCTTCTGCAACTGGATAACTAGCAC 7 0.17500000000000002 No Hit GTTATTGTGAGAATTCTTAATTCAAGAGTTGTAAGGAGGGACTTATGTCA 7 0.17500000000000002 No Hit GAGAATTCTTAATTCAAGAGTTGTAAGGAGGGACTTATGTCACCACAAAC 7 0.17500000000000002 No Hit CAGGGAAACTACTGAAAATGAATCTGCTAATGAGGGTTACAAATTTGGTC 7 0.17500000000000002 No Hit CTTATAATATTGTGGCTGCTCATGGTTATTTTGGCCGATTAATCTTCCAA 7 0.17500000000000002 No Hit GGCCGATTAATCTTCCAATATGCTAGTTTCAACAACTCTCGTTCTTTACA 7 0.17500000000000002 No Hit CGGCGGTTCCCTATTCAGTGCTATGCATGGTTCCTTGGTAACCTCTAGTT 6 0.15 No Hit ATTCCTACTTCTGCGGCAATCGGATTGCACTTTTACCCAATTTGGGAAGC 6 0.15 No Hit GCTCATGGTTATTTTGGCCGATTAATCTTCCAATATGCTAGTTTCAACAA 6 0.15 No Hit CGGATTGCACTTTTACCCAATTTGGGAAGCTGCATCCGTTGATGAATGGT 6 0.15 No Hit GTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGATCTT 6 0.15 No Hit CTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCTAAATGGAT 6 0.15 No Hit AATCTTCCAATATGCTAGTTTCAACAACTCTCGTTCTTTACACTTCTTCT 6 0.15 No Hit GAACTTAGTTTCCGTTTGGGTATGCGCCCTTGGATTGCTGTTGCATATTC 6 0.15 No Hit CTGCTAATGAGGGTTACAAATTTGGTCAAGAGGAAGAGACTTATAATATT 6 0.15 No Hit CCTATTCAGTGCTATGCATGGTTCCTTGGTAACCTCTAGTTTGATCAGGG 6 0.15 No Hit GTTATTAATACTTGGGCTGATATCATCAACCGTGCTAATCTTGGTATGGA 6 0.15 No Hit ATTCAGCTCCTGTTGCAGCTGCGACTGCTGTTTTCTTGATTTACCCTATT 6 0.15 No Hit GTCCTTGCAATTTGAATAAACCAAGATCTTACCATGACTGCAATTTTAGA 6 0.15 No Hit ATTGTATTCCAGGCAGAGCACAACATCCTTATGCATCCATTTCACATGTT 6 0.15 No Hit GTGTTTTCGGCGGTTCCCTATTCAGTGCTATGCATGGTTCCTTGGTAACC 6 0.15 No Hit GTTTGATCAGGGAAACTACTGAAAATGAATCTGCTAATGAGGGTTACAAA 6 0.15 No Hit CCTTGGTAACCTCTAGTTTGATCAGGGAAACTACTGAAAATGAATCTGCT 6 0.15 No Hit GAATGGTTATACAATGGTGGTCCTTATGAGCTAATTGTTCTACACTTCTT 6 0.15 No Hit GGTACTTTCAACTTTATGATTGTATTCCAGGCAGAGCACAACATCCTTAT 6 0.15 No Hit GTTCTTTACTTTATGGAAACAATATTATCTCTGGTGCTATTATTCCTACT 6 0.15 No Hit CTATTCAGTGCTATGCATGGTTCCTTGGTAACCTCTAGTTTGATCAGGGA 5 0.125 No Hit GTAGCTGGTGTTTTCGGCGGTTCCCTATTCAGTGCTATGCATGGTTCCTT 5 0.125 No Hit GGTCGCTTCTGCAACTGGATAACTAGCACTGAAAATCGTCTTTACATCGG 5 0.125 No Hit ATCGGATTGCACTTTTACCCAATTTGGGAAGCTGCATCCGTTGATGAATG 5 0.125 No Hit GTCCTTATGAGCTAATTGTTCTACACTTCTTACTTGGTGTAGCTTGTTAT 5 0.125 No Hit GTCTTTACATCGGATGGTTCGGTGTTTTGATGATCCCTACCTTATTGACC 5 0.125 No Hit ATCGCCTTCATCGCAGCCCCTCCAGTAGATATTGATGGTATTCGCGAGCC 5 0.125 No Hit GGAATATCTGGTACTTTCAACTTTATGATTGTATTCCAGGCAGAGCACAA 5 0.125 No Hit AGAGCACAACATCCTTATGCATCCATTTCACATGTTAGGTGTAGCTGGTG 5 0.125 No Hit TTATAATATTGTGGCTGCTCATGGTTATTTTGGCCGATTAATCTTCCAAT 5 0.125 No Hit GGTTATTTTGGCCGATTAATCTTCCAATATGCTAGTTTCAACAACTCTCG 5 0.125 No Hit TGAAAATGAATCTGCTAATGAGGGTTACAAATTTGGTCAAGAGGAAGAGA 5 0.125 No Hit CTCTAGTTTGATCAGGGAAACTACTGAAAATGAATCTGCTAATGAGGGTT 5 0.125 No Hit GGCCTGTAGTAGGAATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCG 5 0.125 No Hit CGCAGCCCCTCCAGTAGATATTGATGGTATTCGCGAGCCTGTTTCTGGTT 5 0.125 No Hit CTTCTTGGCTGCTTGGCCTGTAGTAGGAATCTGGTTCACTGCTTTAGGTA 5 0.125 No Hit AGTCAAGGTCGCGTTATTAATACTTGGGCTGATATCATCAACCGTGCTAA 5 0.125 No Hit CGGATGGTTCGGTGTTTTGATGATCCCTACCTTATTGACCGCAACTTCTG 5 0.125 No Hit GTCAAGGTCGCGTTATTAATACTTGGGCTGATATCATCAACCGTGCTAAT 5 0.125 No Hit GGTTCTTTACTTTATGGAAACAATATTATCTCTGGTGCTATTATTCCTAC 5 0.125 No Hit TGCAATTTGAATAAACCAAGATCTTACCATGACTGCAATTTTAGAGAGAC 5 0.125 No Hit GATTTACCCTATTGGTCAAGGAAGCTTCTCTGATGGTATGCCTTTAGGAA 5 0.125 No Hit GATCGTTTTGTCTTTTGTGCCGAAGCTATTTATAAATCACAGGCGGAAAC 5 0.125 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.025 0.0 0.0 0.0 0.0 62-63 0.025 0.0 0.0 0.0 0.0 64-65 0.05 0.0 0.0 0.0 0.0 66-67 0.05 0.0 0.0 0.0 0.0 68-69 0.05 0.0 0.0 0.0 0.0 70-71 0.075 0.0 0.0 0.0 0.0 72-73 0.075 0.0 0.0 0.0 0.0 74-75 0.075 0.0 0.0 0.0 0.0 76-77 0.075 0.0 0.0 0.0 0.0 78-79 0.075 0.0 0.0 0.0 0.0 80-81 0.1 0.0 0.0 0.0 0.0 82-83 0.125 0.0 0.0 0.0 0.0 84-85 0.1875 0.0 0.0 0.0 0.0 86-87 0.3375 0.0 0.0 0.0 0.0 88-89 0.42500000000000004 0.0 0.0 0.0 0.0 90-91 0.5625 0.0 0.0 0.0 0.0 92-93 0.7875 0.0 0.0 0.0 0.0 94-95 0.925 0.0 0.0 0.0 0.0 96-97 1.0375 0.0 0.0 0.0 0.0 98-99 1.125 0.0 0.0 0.0 0.0 100-101 1.3 0.0 0.0 0.0 0.0 102-103 1.5375 0.0 0.0 0.0 0.0 104-105 1.7625 0.0 0.0 0.0 0.0 106-107 1.95 0.0 0.0 0.0 0.0 108-109 2.1500000000000004 0.0 0.0 0.0 0.0 110-111 2.3 0.0 0.0 0.0 0.0 112-113 2.5875 0.0 0.0 0.0 0.0 114-115 2.8375 0.0 0.0 0.0 0.0 116-117 3.125 0.0 0.0 0.0 0.0 118-119 3.4125 0.0 0.0 0.0 0.0 120-121 3.7625 0.0 0.0 0.0 0.0 122-123 4.137499999999999 0.0 0.0 0.0 0.0 124-125 4.5875 0.0 0.0 0.0 0.0 126-127 4.925 0.0 0.0 0.0 0.0 128-129 5.225 0.0 0.0 0.0 0.0 130-131 5.6375 0.0 0.0 0.0 0.0 132-133 6.025 0.0 0.0 0.0 0.0 134-135 6.5 0.0 0.0 0.0 0.0 136-137 6.975 0.0 0.0 0.0 0.0 138-139 7.324999999999999 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content pass >>END_MODULE Read 1323686 spots for SRR6941560.sra Written 1323686 spots for SRR6941560.sra Read 1323686 spots for SRR6941560.sra Written 1323686 spots for SRR6941560.sra Read 1323686 spots for SRR6941560.sra Written 1323686 spots for SRR6941560.sra Read 1323686 spots for SRR6941560.sra Written 1323686 spots for SRR6941560.sra Read 1323686 spots for SRR6941560.sra Written 1323686 spots for SRR6941560.sra Read 1323686 spots for SRR6941560.sra Written 1323686 spots for SRR6941560.sra Read 1323686 spots for SRR6941560.sra Written 1323686 spots for SRR6941560.sra Read 1323686 spots for SRR6941560.sra Written 1323686 spots for SRR6941560.sra Read 1323686 spots for SRR6941560.sra Written 1323686 spots for SRR6941560.sra Read 1323705 spots for SRR6941560.sra Written 1323705 spots for SRR6941560.sra Read 1323686 spots for SRR6941560.sra Written 1323686 spots for SRR6941560.sra Read 1323686 spots for SRR6941560.sra Written 1323686 spots for SRR6941560.sra Read 1323686 spots for SRR6941560.sra Written 1323686 spots for SRR6941560.sra Read 1323686 spots for SRR6941560.sra Written 1323686 spots for SRR6941560.sra Read 1323686 spots for SRR6941560.sra Written 1323686 spots for SRR6941560.sra Read 1323686 spots for SRR6941560.sra Written 1323686 spots for SRR6941560.sra Read 1323686 spots for SRR6941560.sra Written 1323686 spots for SRR6941560.sra Read 1323686 spots for SRR6941560.sra Written 1323686 spots for SRR6941560.sra Read 1323686 spots for SRR6941560.sra Written 1323686 spots for SRR6941560.sra Read 1323686 spots for SRR6941560.sra Written 1323686 spots for SRR6941560.sra SRR ids: ['SRR6941560.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_bqxxrdev SRR6941560.sra spots: 26473739 blocks: [[1, 1323686], [1323687, 2647372], [2647373, 3971058], [3971059, 5294744], [5294745, 6618430], [6618431, 7942116], [7942117, 9265802], [9265803, 10589488], [10589489, 11913174], [11913175, 13236860], [13236861, 14560546], [14560547, 15884232], [15884233, 17207918], [17207919, 18531604], [18531605, 19855290], [19855291, 21178976], [21178977, 22502662], [22502663, 23826348], [23826349, 25150034], [25150035, 26473739]] SRR6941560 file size 8949381 SRR6941560 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR6941560 SRR6941560_1.fastq SRR6941560_2.fastq Input file: SRR6941560_1.fastq Paired file: SRR6941560_2.fastq trimmed: SRR6941560-trimmed-pair1.fastq, SRR6941560-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Fri Dec 6 11:23:30 2024 >> started Fri Dec 6 11:23:56 2024 >> done (26.036s) 26473739 read pairs processed; of these: 7227 ( 0.03%) short read pairs filtered out after trimming by size control 9130 ( 0.03%) empty read pairs filtered out after trimming by size control 26457382 (99.94%) read pairs available; of these: 4505225 (17.03%) trimmed read pairs available after processing 21952157 (82.97%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 3 0.00% 19 2 0.00% 20 3 0.00% 21 1 0.00% 22 5 0.00% 23 2 0.00% 24 4 0.00% 25 4 0.00% 26 4 0.00% 27 9 0.00% 28 6 0.00% 29 13 0.00% 30 11 0.00% 31 8 0.00% 32 9 0.00% 33 4 0.00% 34 20 0.00% 35 17 0.00% 36 22 0.00% 37 16 0.00% 38 31 0.00% 39 36 0.00% 40 42 0.00% 41 60 0.00% 42 51 0.00% 43 68 0.00% 44 77 0.00% 45 59 0.00% 46 92 0.00% 47 100 0.00% 48 118 0.00% 49 135 0.00% 50 182 0.00% 51 187 0.00% 52 241 0.00% 53 238 0.00% 54 264 0.00% 55 301 0.00% 56 334 0.00% 57 445 0.00% 58 471 0.00% 59 492 0.00% 60 593 0.00% 61 686 0.00% 62 902 0.00% 63 997 0.00% 64 1158 0.00% 65 1336 0.01% 66 1310 0.00% 67 1603 0.01% 68 1714 0.01% 69 2063 0.01% 70 2340 0.01% 71 2869 0.01% 72 3260 0.01% 73 3635 0.01% 74 3601 0.01% 75 4455 0.02% 76 4183 0.02% 77 5042 0.02% 78 5034 0.02% 79 5448 0.02% 80 6188 0.02% 81 7010 0.03% 82 8303 0.03% 83 8606 0.03% 84 9513 0.04% 85 11354 0.04% 86 11549 0.04% 87 12438 0.05% 88 13941 0.05% 89 14278 0.05% 90 16196 0.06% 91 16369 0.06% 92 19403 0.07% 93 19350 0.07% 94 21396 0.08% 95 23141 0.09% 96 21680 0.08% 97 21746 0.08% 98 22029 0.08% 99 23554 0.09% 100 24223 0.09% 101 26347 0.10% 102 29555 0.11% 103 28910 0.11% 104 31312 0.12% 105 33510 0.13% 106 34194 0.13% 107 33494 0.13% 108 33471 0.13% 109 37377 0.14% 110 36782 0.14% 111 41241 0.16% 112 42487 0.16% 113 40116 0.15% 114 44148 0.17% 115 40914 0.15% 116 42251 0.16% 117 41339 0.16% 118 41369 0.16% 119 42279 0.16% 120 43672 0.17% 121 44338 0.17% 122 49538 0.19% 123 50640 0.19% 124 51909 0.20% 125 53812 0.20% 126 51197 0.19% 127 51070 0.19% 128 51644 0.20% 129 58279 0.22% 130 57295 0.22% 131 60478 0.23% 132 61916 0.23% 133 54779 0.21% 134 63557 0.24% 135 59799 0.23% 136 63312 0.24% 137 60580 0.23% 138 67216 0.25% 139 70396 0.27% 140 67822 0.26% 141 82058 0.31% 142 71047 0.27% 143 77963 0.29% 144 74767 0.28% 145 86243 0.33% 146 92439 0.35% 147 101264 0.38% 148 127650 0.48% 149 188468 0.71% 150 1320298 4.99% 151 21952157 82.97% 26457382 reads passed initial QC criterion=sequence-density sequence-density=0.31 sequence-density-rank=1 fanout-score=1.95 fanout-score-rank=31 prefix-density=0.30 prefix-fanout=2.0 sequence=TCTAATTCAAAA criterion=fanout-score sequence-density=0.01 sequence-density-rank=36 fanout-score=70.25 fanout-score-rank=1 prefix-density=0.35 prefix-fanout=2.4 sequence=AGAAAAAAACAAGTTTGCATCTTCAGGAGAATCTATATTTTCGCGAAATGGATCATAATAAAATGGATTTTAGGTATCTAGGGAAAATTCACTTCGAAGTAACTATTTCCTAGATACCTATGCACGGTACTTCACGGTTGAATGAATCAACCTGAAAAATACCTAAAAAAGGCCTAAAGTTAAGGATTTATCAATGGGTAATGTTGCTCCAATACCTAACCAAAGAGCTACTGCAGTACCGATTAAAAAAACGGTCGTAGCTACTGGGCGACGAAATGGATTTTGGAATTTGTTGACATTCTCTAGAAAAGGTACTGTCAATAAGCCTGTTGGCACAGAAACCATTAAGAGAACGCCCAATAACTTATTGGGTACCGTACGGAGTATTTGAAACACGGGAAAGAAGTACCACTCGGGTAATATTTCCAAAGGAGTTGCAAACGGATCCGCGGGTTCACCAATCATTGATGGCTCGAGAACAGCTAAACCTACATTACATGCAATAGTACCT criterion=sequence-density sequence-density=0.28 sequence-density-rank=1 fanout-score=1.95 fanout-score-rank=30 prefix-density=0.28 prefix-fanout=2.0 sequence=GCGGCTCTCCTA criterion=fanout-score sequence-density=0.05 sequence-density-rank=27 fanout-score=18.12 fanout-score-rank=1 prefix-density=0.86 prefix-fanout=1.1 sequence=AAAAGATCGTGTATTTACAACTACAACGGAATAGTATACAAAGTCAACACCAACGATTAAATTGAATTTATGGCTACGCAAACCGTTGAAGATAGTTCTAAACCTAAACCAAGACGAACTGGTGCAGGTAGTTTATTGAAACCCTTGAATTCGGAATATGGGAAAGTAGCTCCAGGTTGGGGGACTACTCCTTTTATGGGGGTCGCAATGGCTTTATTCGCGATATTCCTATCTATCATTTTAGAAATTTATAATTCTTCTGTTTTATTGGACGGAATTTTAACCTATTAGGTTTCTACTAACTAAAAGTACGAAGTCGTAGTTTTTCCATCCAAAAAAAGCCTTTCTAGTTTAAGCTCTACATTTCTAGACATTCTGGTAGTTCGACCGCGGAATTTTTTTGTTTCGGTATCTCTGGAATATGAGTAAAGTATATGATTGGTTTGAGGAACGTCTTGAGATTCAGGCAATTGCAGATGATATAACTAGTAAATATGTTCCTCCTCATGTC SRR6941560 testing PE reads STAR mapping to Ensembl genome Started job on | Dec 06 11:24:37 Started mapping on | Dec 06 11:24:38 Finished on | Dec 06 11:26:38 Mapping speed, Million of reads per hour | 793.72 Number of input reads | 26457382 Average input read length | 295 UNIQUE READS: Uniquely mapped reads number | 15878147 Uniquely mapped reads % | 60.01% Average mapped length | 296.47 Number of splices: Total | 3122009 Number of splices: Annotated (sjdb) | 2781344 Number of splices: GT/AG | 2925606 Number of splices: GC/AG | 35844 Number of splices: AT/AC | 16905 Number of splices: Non-canonical | 143654 Mismatch rate per base, % | 0.26% Deletion rate per base | 0.02% Deletion average length | 1.97 Insertion rate per base | 0.02% Insertion average length | 2.68 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 9217330 % of reads mapped to multiple loci | 34.84% Number of reads mapped to too many loci | 24072 % of reads mapped to too many loci | 0.09% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 4.58% % of reads unmapped: other | 0.48% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 1369068 1369068 1369068 N_multimapping 9217330 9217330 9217330 N_noFeature 6329837 15190274 6633735 N_ambiguous 716348 10698 344833 UnstrandedReadsAssigned:8831962 PositiveStrandReadsAssigned:677175 NegativeStrandReadsAssigned:8899579 Dataset is classified negative stranded MeadianReadLen=151 20thPercentileLength=151 echo kmer=147 SRR6941560 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,972 [index] number of k-mers: 66,720,672 [index] number of equivalence classes: 111,837 [quant] running in paired-end mode [quant] will process pair 1: SRR6941560-trimmed-pair1.fastq SRR6941560-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 26,457,382 reads, 15,164,507 reads pseudoaligned [quant] estimated average fragment length: 246.798 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,036 rounds 52973 SRR6941560.ke.tsv 35125 SRR6941560.se.tsv 88098 total ==> SRR6941560.ke.tsv <== target_id length eff_length est_counts tpm PNS24245 936 690.603 0 0 PNS24247 1044 798.202 5.36823 0.435378 PNS24249 1928 1682.2 10.9076 0.419756 PNS24246 1044 798.202 5.36823 0.435378 PNS24248 1044 798.202 5.36823 0.435378 PNS24244 1471 1225.2 22.9878 1.21461 PNS24243 293 98.3134 0 0 KQK14069 1603 1357.2 286.439 13.6627 KQK14071 474 240.541 4.96876 1.33723 ==> SRR6941560.se.tsv <== BRADI_1g14170v3 443 BRADI_1g53295v3 50 BRADI_1g59795v3 12 BRADI_1g07683v3 0 BRADI_1g00485v3 1 BRADI_1g20270v3 99 BRADI_1g74790v3 8 BRADI_1g09890v3 0 BRADI_1g77505v3 50 BRADI_1g48960v3 0 SRR6941560 completed mapping pipeline successfully