Starting /dee2/code/volunteer_pipeline.sh SRR6941561 current disk space = 1551121915904 free memory = 1313262188 SRR6941561 SRAfilesize d2aecbcf2616d4a0f5b2b567dafe7ea2 SRR6941561.sra SRR6941561.sra file validated SRR6941561 is paired end SRR6941561 is conventional basespace SRR6941561 read1 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR6941561_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 34.70425 35.0 35.0 35.0 35.0 35.0 2 34.63375 35.0 35.0 35.0 35.0 35.0 3 34.68825 35.0 35.0 35.0 35.0 35.0 4 34.72125 35.0 35.0 35.0 35.0 35.0 5 34.65925 35.0 35.0 35.0 35.0 35.0 6 39.51325 40.0 40.0 40.0 39.0 40.0 7 39.51325 40.0 40.0 40.0 39.0 40.0 8 39.51075 40.0 40.0 40.0 39.0 40.0 9 39.56225 40.0 40.0 40.0 39.0 40.0 10-14 39.524950000000004 40.0 40.0 40.0 39.0 40.0 15-19 39.4555 40.0 40.0 40.0 39.0 40.0 20-24 39.50145 40.0 40.0 40.0 39.0 40.0 25-29 39.474450000000004 40.0 40.0 40.0 39.0 40.0 30-34 39.32275 40.0 40.0 40.0 39.0 40.0 35-39 39.443149999999996 40.0 40.0 40.0 39.0 40.0 40-44 39.42 40.0 40.0 40.0 39.0 40.0 45-49 39.3994 40.0 40.0 40.0 39.0 40.0 50-54 39.3606 40.0 40.0 40.0 39.0 40.0 55-59 39.32785 40.0 40.0 40.0 39.0 40.0 60-64 39.37585 40.0 40.0 40.0 39.0 40.0 65-69 39.317099999999996 40.0 40.0 40.0 39.0 40.0 70-74 39.2168 40.0 40.0 40.0 38.8 40.0 75-79 39.1292 40.0 40.0 40.0 38.8 40.0 80-84 39.21295 40.0 40.0 40.0 39.0 40.0 85-89 39.16085 40.0 40.0 40.0 39.0 40.0 90-94 39.130050000000004 40.0 40.0 40.0 38.6 40.0 95-99 39.1289 40.0 40.0 40.0 38.6 40.0 100-104 38.2238 39.2 38.6 39.4 36.8 39.8 105-109 39.10385 40.0 39.8 40.0 38.6 40.0 110-114 39.1527 40.0 40.0 40.0 38.6 40.0 115-119 39.2438 40.0 40.0 40.0 39.0 40.0 120-124 39.0215 40.0 40.0 40.0 38.2 40.0 125-129 38.913500000000006 40.0 39.4 40.0 38.0 40.0 130-134 38.8941 40.0 39.2 40.0 37.8 40.0 135-139 38.767900000000004 40.0 39.0 40.0 37.2 40.0 140-144 38.627849999999995 40.0 39.0 40.0 36.6 40.0 145-149 38.714150000000004 40.0 39.0 40.0 37.2 40.0 150-151 36.979 39.5 37.5 40.0 33.0 40.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 16 1.0 17 0.0 18 0.0 19 0.0 20 0.0 21 2.0 22 1.0 23 2.0 24 3.0 25 5.0 26 4.0 27 8.0 28 10.0 29 12.0 30 20.0 31 30.0 32 36.0 33 37.0 34 35.0 35 77.0 36 68.0 37 121.0 38 249.0 39 3279.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 44.2663995993991 10.040060090135203 5.983975963945919 39.709564346519784 2 23.549999999999997 13.450000000000001 31.3 31.7 3 21.7 17.7 25.35 35.25 4 25.75 25.2 21.75 27.3 5 25.056376847907792 30.117764971185167 23.227261338010525 21.598596842896516 6 19.275000000000002 35.475 23.974999999999998 21.275 7 13.55 28.725 40.925 16.8 8 17.925 26.1 31.525 24.45 9 17.025000000000002 23.375 35.55 24.05 10-14 19.86 31.495 25.580000000000002 23.064999999999998 15-19 21.035 29.304999999999996 25.224999999999998 24.435000000000002 20-24 19.15191519151915 30.04300430043004 26.932693269326936 23.87238723872387 25-29 22.314999999999998 29.435 26.150000000000002 22.1 30-34 22.695 30.78 24.14 22.384999999999998 35-39 21.415 29.970000000000002 26.19 22.425 40-44 20.215 29.615000000000002 26.540000000000003 23.630000000000003 45-49 20.235 28.505000000000003 27.765 23.494999999999997 50-54 21.145 28.53 27.195000000000004 23.13 55-59 21.455 28.46 25.615 24.47 60-64 20.18 29.255 26.63 23.935000000000002 65-69 20.435 30.28 25.485000000000003 23.799999999999997 70-74 20.995 29.48 24.125 25.4 75-79 20.995 29.044999999999998 26.290000000000003 23.669999999999998 80-84 21.89 28.99 24.985 24.135 85-89 22.39 28.175 25.845000000000002 23.59 90-94 20.419999999999998 29.505 26.295 23.78 95-99 21.27 29.470000000000002 24.055 25.205 100-104 20.845 30.425 25.580000000000002 23.150000000000002 105-109 20.275000000000002 29.520000000000003 25.995 24.21 110-114 20.61 29.465000000000003 25.790000000000003 24.135 115-119 20.580000000000002 30.404999999999998 24.21 24.805 120-124 20.98049024512256 29.67983991995998 23.65182591295648 25.68784392196098 125-129 20.407040704070408 30.59305930593059 23.75237523752375 25.247524752475247 130-134 21.795 30.020000000000003 23.845 24.34 135-139 22.6 29.435 24.740000000000002 23.225 140-144 23.34 28.599999999999998 24.97 23.09 145-149 20.555 30.325000000000003 24.525 24.595 150-151 20.080120180270406 30.696044066099148 24.649474211316978 24.57436154231347 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.5 4 0.5 5 0.5 6 0.5 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 1.0 19 2.0 20 1.5 21 1.0 22 2.5 23 3.5 24 4.0 25 3.5 26 5.0 27 10.0 28 15.5 29 13.0 30 8.5 31 14.0 32 20.5 33 24.0 34 31.0 35 40.5 36 82.0 37 196.0 38 239.5 39 212.0 40 256.5 41 270.5 42 200.5 43 208.5 44 222.5 45 198.5 46 182.5 47 145.5 48 122.5 49 90.0 50 80.0 51 76.0 52 66.5 53 79.0 54 102.5 55 134.5 56 141.0 57 102.5 58 83.5 59 80.0 60 62.5 61 41.5 62 26.0 63 26.5 64 20.5 65 13.0 66 11.0 67 5.5 68 5.0 69 4.5 70 3.0 71 2.0 72 1.0 73 0.5 74 0.0 75 1.0 76 1.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.15 2 0.0 3 0.0 4 0.0 5 0.22499999999999998 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.01 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.05 125-129 0.01 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.15 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.724999999999994 #Duplication Level Percentage of deduplicated Percentage of total 1 82.82184137106417 51.949999999999996 2 8.051016341171783 10.100000000000001 3 3.1885213232363494 6.0 4 1.315265045834994 3.3000000000000003 5 0.9565563969709047 3.0 6 0.7572738142686329 2.85 7 0.5579912315663611 2.45 8 0.5978477481068154 3.0 9 0.27899561578318055 1.575 >10 1.4746911119968116 15.775 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GCGGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTACG 47 1.175 No Hit CTTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCAT 44 1.0999999999999999 No Hit GGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTACGTT 35 0.8750000000000001 No Hit GCTTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCA 33 0.8250000000000001 No Hit GGCCAAAATAACCATGAGCAGCCACAATATTATAAGTCTCTTCCTCTTGA 31 0.775 No Hit CCCAAGTATTAATAACGCGACCTTGACTATCAACTACAGATTGGTTGAAA 27 0.675 No Hit GTGCTCTGCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTCCTA 26 0.65 No Hit CTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCATTAAT 23 0.575 No Hit GTTAGCGGAAAAACCTTATCCATTAATAGCGGGAACTTCAAGAGCAGCTA 22 0.5499999999999999 No Hit CGGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTACGT 21 0.525 No Hit CTCATAAGGACCACCATTGTATAACCATTCATCAACGGATGCAGCTTCCC 18 0.44999999999999996 No Hit GGATGCATAAGGATGTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAA 16 0.4 No Hit GCCGAAAACACCAGCTACACCTAACATGTGAAATGGATGCATAAGGATGT 15 0.375 No Hit CCAGATTCCTACTACAGGCCAAGCAGCCAAGAAGAAGTGTAAAGAACGAG 15 0.375 No Hit CTCTTGACCAAATTTGTAACCCTCATTAGCAGATTCATTTTCAGTAGTTT 13 0.325 No Hit GACCCATATAACAAGCTACACCAAGTAAGAAGTGTAGAACAATTAGCTCA 13 0.325 No Hit GCTCTGCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTCCTAAA 13 0.325 No Hit GTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTC 12 0.3 No Hit CATATAACAAGCTACACCAAGTAAGAAGTGTAGAACAATTAGCTCATAAG 12 0.3 No Hit GATGCATAAGGATGTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAAA 12 0.3 No Hit GCGGAAAAACCTTATCCATTAATAGCGGGAACTTCAAGAGCAGCTAGATC 12 0.3 No Hit CGCGACCTTGACTATCAACTACAGATTGGTTGAAATTGAATCCATTTAGG 12 0.3 No Hit GCCAAGAAGAAGTGTAAAGAACGAGAGTTGTTGAAACTAGCATATTGGAA 12 0.3 No Hit GCACGGTTGATGATATCAGCCCAAGTATTAATAACGCGACCTTGACTATC 11 0.27499999999999997 No Hit GTGAGCATTACGTTCGTGCATTACTTCCATACCAAGATTAGCACGGTTGA 11 0.27499999999999997 No Hit GCGACCTTGACTATCAACTACAGATTGGTTGAAATTGAATCCATTTAGGT 11 0.27499999999999997 No Hit AGCGGAAAAACCTTATCCATTAATAGCGGGAACTTCAAGAGCAGCTAGAT 11 0.27499999999999997 No Hit GCCAAAATAACCATGAGCAGCCACAATATTATAAGTCTCTTCCTCTTGAC 11 0.27499999999999997 No Hit GGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTACGTTC 11 0.27499999999999997 No Hit GGGGCTGCGATGAAGGCGATAATAAATACAGAAGTTGCGGTCAATAAGGT 11 0.27499999999999997 No Hit CGCAGAAGTAGGAATAATAGCACCAGAGATAATATTGTTTCCATAAAGTA 10 0.25 No Hit GCTAGTTATCCAGTTGCAGAAGCGACCCCACAGGCTTGTACTTTCGCGTC 10 0.25 No Hit GCTGCGATGAAGGCGATAATAAATACAGAAGTTGCGGTCAATAAGGTAGG 10 0.25 No Hit CCCACTGCTGCCTCCCGTAGGAGTCTGGGCCGTGTCTCAGTCCCAGTGTG 10 0.25 No Hit CCACAATATTATAAGTCTCTTCCTCTTGACCAAATTTGTAACCCTCATTA 10 0.25 No Hit GTTGAAATTGAATCCATTTAGGTTGAACGCCATAGTACTAATACCTAAAG 10 0.25 No Hit CTCTGCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTCCTAAAG 10 0.25 No Hit GGAGGGGCTGCGATGAAGGCGATAATAAATACAGAAGTTGCGGTCAATAA 9 0.22499999999999998 No Hit CTGCGATGAAGGCGATAATAAATACAGAAGTTGCGGTCAATAAGGTAGGG 9 0.22499999999999998 No Hit CGAGAGTTGTTGAAACTAGCATATTGGAAGATTAATCGGCCAAAATAACC 9 0.22499999999999998 No Hit GGCGATAATAAATACAGAAGTTGCGGTCAATAAGGTAGGGATCATCAAAA 9 0.22499999999999998 No Hit CTCATTAGCAGATTCATTTTCAGTAGTTTCCCTGATCAAACTAGAGGTTA 9 0.22499999999999998 No Hit CTCCACGCGGCATTGCTCCGTCAGGCTTTCGCCCATTGCGGAAAATTCCC 9 0.22499999999999998 No Hit GTTGAAACTAGCATATTGGAAGATTAATCGGCCAAAATAACCATGAGCAG 9 0.22499999999999998 No Hit GGTTGATGATATCAGCCCAAGTATTAATAACGCGACCTTGACTATCAACT 8 0.2 No Hit GGCGGAAAAACCTTATCCATTAATAGCGGGAACTTCAAGAGCAGCTAGAT 8 0.2 No Hit GGCATGATGACTTGGCCTCATCCTCTCCTTCCTCCGGCTTAACACCGGCG 8 0.2 No Hit TTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCATT 8 0.2 No Hit CCATATAACAAGCTACACCAAGTAAGAAGTGTAGAACAATTAGCTCATAA 8 0.2 No Hit TTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCATTA 8 0.2 No Hit CCCTCATTAGCAGATTCATTTTCAGTAGTTTCCCTGATCAAACTAGAGGT 8 0.2 No Hit GGATGTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAAAGTACCAGAT 8 0.2 No Hit CATCAGAGAAGCTTCCTTGACCAATAGGGTAAATCAAGAAAACAGCAGTC 8 0.2 No Hit CCAAGTATTAATAACGCGACCTTGACTATCAACTACAGATTGGTTGAAAT 8 0.2 No Hit GGCCAAGCAGCCAAGAAGAAGTGTAAAGAACGAGAGTTGTTGAAACTAGC 8 0.2 No Hit CATAAGGATGTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAAAGTAC 8 0.2 No Hit CATCAATATCTACTGGAGGGGCTGCGATGAAGGCGATAATAAATACAGAA 8 0.2 No Hit CCGGGAACGGATTCACCGCCGTATGGCTGACCGGCGATTACTAGCGATTC 8 0.2 No Hit CCCCACTGCTGCCTCCCGTAGGAGTCTGGGCCGTGTCTCAGTCCCAGTGT 8 0.2 No Hit ACCAGCTACACCTAACATGTGAAATGGATGCATAAGGATGTTGTGCTCTG 7 0.17500000000000002 No Hit CCCATATAACAAGCTACACCAAGTAAGAAGTGTAGAACAATTAGCTCATA 7 0.17500000000000002 No Hit CTTCCTCTTGACCAAATTTGTAACCCTCATTAGCAGATTCATTTTCAGTA 7 0.17500000000000002 No Hit CAGAGAAGCTTCCTTGACCAATAGGGTAAATCAAGAAAACAGCAGTCGCA 7 0.17500000000000002 No Hit GGCGCATACCCAAACGGAAACTAAGTTCCCACTCACGACCCATATAACAA 7 0.17500000000000002 No Hit TCTGCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTCCTAAAGG 7 0.17500000000000002 No Hit GCATTACGTTCGTGCATTACTTCCATACCAAGATTAGCACGGTTGATGAT 7 0.17500000000000002 No Hit GATGATATCAGCCCAAGTATTAATAACGCGACCTTGACTATCAACTACAG 7 0.17500000000000002 No Hit CCCGTCAATTCCTTTGAGTTTCATTCTTGCGAACGTACTCCCCAGGCGGG 7 0.17500000000000002 No Hit TGCATAAGGATGTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAAAGT 7 0.17500000000000002 No Hit GTTGATGATATCAGCCCAAGTATTAATAACGCGACCTTGACTATCAACTA 7 0.17500000000000002 No Hit GCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTCCTAAAGGCAT 7 0.17500000000000002 No Hit CTTTCTTTTCCTCTGGCTACTAAGATGTTTCAGTTCGCCAGGTTGTCTCT 7 0.17500000000000002 No Hit CTTATCCATTAATAGCGGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAG 7 0.17500000000000002 No Hit CTCCACCGCTTGTGCGGGCCCCCGTCAATTCCTTTGAGTTTCATTCTTGC 6 0.15 No Hit GTCGCAGCTGCAACAGGAGCTGAATATGCAACAGCAATCCAAGGGCGCAT 6 0.15 No Hit CCTCACGGTACTACTTCGCTATCGGTCACCCAGGAGTATTTAGCCTTGCA 6 0.15 No Hit CCACCATTGTATAACCATTCATCAACGGATGCAGCTTCCCAAATTGGGTA 6 0.15 No Hit CCAAGAAGAAGTGTAAAGAACGAGAGTTGTTGAAACTAGCATATTGGAAG 6 0.15 No Hit ACATGCTCCACCGCTTGTGCGGGCCCCCGTCAATTCCTTTGAGTTTCATT 6 0.15 No Hit CGGGAACGGATTCACCGCCGTATGGCTGACCGGCGATTACTAGCGATTCC 6 0.15 No Hit TTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCATTAATA 6 0.15 No Hit CTAACATGTGAAATGGATGCATAAGGATGTTGTGCTCTGCCTGGAATACA 6 0.15 No Hit GGTCAATAAGGTAGGGATCATCAAAACACCGAACCATCCGATGTAAAGAC 6 0.15 No Hit GAGTTGTTGAAACTAGCATATTGGAAGATTAATCGGCCAAAATAACCATG 6 0.15 No Hit GTAAAGAACGAGAGTTGTTGAAACTAGCATATTGGAAGATTAATCGGCCA 6 0.15 No Hit CTAGCATATTGGAAGATTAATCGGCCAAAATAACCATGAGCAGCCACAAT 6 0.15 No Hit GCTTTCTTTTCCTCTGGCTACTAAGATGTTTCAGTTCGCCAGGTTGTCTC 6 0.15 No Hit GCTAGATCTAGAGGGAAGTTGTGAGCATTACGTTCGTGCATTACTTCCAT 6 0.15 No Hit CACGGTTGATGATATCAGCCCAAGTATTAATAACGCGACCTTGACTATCA 6 0.15 No Hit GTAGGAATAATAGCACCAGAGATAATATTGTTTCCATAAAGTAAAGAACC 6 0.15 No Hit AGCAGATTCATTTTCAGTAGTTTCCCTGATCAAACTAGAGGTTACCAAGG 6 0.15 No Hit GAGCATTACGTTCGTGCATTACTTCCATACCAAGATTAGCACGGTTGATG 6 0.15 No Hit GCGGTCAATAAGGTAGGGATCATCAAAACACCGAACCATCCGATGTAAAG 5 0.125 No Hit GGGCGCATACCCAAACGGAAACTAAGTTCCCACTCACGACCCATATAACA 5 0.125 No Hit GGAAGATTAATCGGCCAAAATAACCATGAGCAGCCACAATATTATAAGTC 5 0.125 No Hit CTTGACTATCAACTACAGATTGGTTGAAATTGAATCCATTTAGGTTGAAC 5 0.125 No Hit ACCAGATATTCCTAAAGGCATACCATCAGAGAAGCTTCCTTGACCAATAG 5 0.125 No Hit GTCGGTTTCGGGTACAGGTACCCTTTTGTTGAAGGTCGTTCGAGCTTTTC 5 0.125 No Hit TGATGATATCAGCCCAAGTATTAATAACGCGACCTTGACTATCAACTACA 5 0.125 No Hit GGATCATCAAAACACCGAACCATCCGATGTAAAGACGATTTTCAGTGCTA 5 0.125 No Hit ATCCATTAATAGCGGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTG 5 0.125 No Hit GTCCGTCTGGCCCGAGGAAACCTTTGCACGCCTCCGTTACCTTTTGGGAG 5 0.125 No Hit CACCTCCACGCGGCATTGCTCCGTCAGGCTTTCGCCCATTGCGGAAAATT 5 0.125 No Hit ATCGTTTACGGCTAGGACTACTGGGGTCTCTAATCCCATTTGCTCCCCTA 5 0.125 No Hit GCATAAGGATGTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAAAGTA 5 0.125 No Hit AGCACGTGTGTCGCCCAGGGCATAAGGGGCATGATGACTTGGCCTCATCC 5 0.125 No Hit TAAAGAACGAGAGTTGTTGAAACTAGCATATTGGAAGATTAATCGGCCAA 5 0.125 No Hit GCCCAAGTATTAATAACGCGACCTTGACTATCAACTACAGATTGGTTGAA 5 0.125 No Hit CGGTCAATAAGGTAGGGATCATCAAAACACCGAACCATCCGATGTAAAGA 5 0.125 No Hit AGTTGTTGAAACTAGCATATTGGAAGATTAATCGGCCAAAATAACCATGA 5 0.125 No Hit CACCATTGTATAACCATTCATCAACGGATGCAGCTTCCCAAATTGGGTAA 5 0.125 No Hit GGTAAATCAAGAAAACAGCAGTCGCAGCTGCAACAGGAGCTGAATATGCA 5 0.125 No Hit CGGCCAAAATAACCATGAGCAGCCACAATATTATAAGTCTCTTCCTCTTG 5 0.125 No Hit GTCAATAAGGTAGGGATCATCAAAACACCGAACCATCCGATGTAAAGACG 5 0.125 No Hit CCATTAATAGCGGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTG 5 0.125 No Hit ATAACAAGCTACACCAAGTAAGAAGTGTAGAACAATTAGCTCATAAGGAC 5 0.125 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.025 0.0 0.0 0.0 0.0 62-63 0.025 0.0 0.0 0.0 0.0 64-65 0.025 0.0 0.0 0.0 0.0 66-67 0.025 0.0 0.0 0.0 0.0 68-69 0.05 0.0 0.0 0.0 0.0 70-71 0.05 0.0 0.0 0.0 0.0 72-73 0.075 0.0 0.0 0.0 0.0 74-75 0.075 0.0 0.0 0.0 0.0 76-77 0.125 0.0 0.0 0.0 0.0 78-79 0.15 0.0 0.0 0.0 0.0 80-81 0.175 0.0 0.0 0.0 0.0 82-83 0.225 0.0 0.0 0.0 0.0 84-85 0.325 0.0 0.0 0.0 0.0 86-87 0.4375 0.0 0.0 0.0 0.0 88-89 0.6 0.0 0.0 0.0 0.0 90-91 0.6875 0.0 0.0 0.0 0.0 92-93 0.8374999999999999 0.0 0.0 0.0 0.0 94-95 1.0 0.0 0.0 0.0 0.0 96-97 1.075 0.0 0.0 0.0 0.0 98-99 1.15 0.0 0.0 0.0 0.0 100-101 1.275 0.0 0.0 0.0 0.0 102-103 1.4249999999999998 0.0 0.0 0.0 0.0 104-105 1.625 0.0 0.0 0.0 0.0 106-107 1.9125 0.0 0.0 0.0 0.0 108-109 2.1625 0.0 0.0 0.0 0.0 110-111 2.5875 0.0 0.0 0.0 0.0 112-113 2.9375 0.0 0.0 0.0 0.0 114-115 3.325 0.0 0.0 0.0 0.0 116-117 3.6 0.0 0.0 0.0 0.0 118-119 3.875 0.0 0.0 0.0 0.0 120-121 4.125 0.0 0.0 0.0 0.0 122-123 4.5125 0.0 0.0 0.0 0.0 124-125 4.887499999999999 0.0 0.0 0.0 0.0 126-127 5.35 0.0 0.0 0.0 0.0 128-129 5.775 0.0 0.0 0.0 0.0 130-131 6.0625 0.0 0.0 0.0 0.0 132-133 6.4875 0.0 0.0 0.0 0.0 134-135 6.925000000000001 0.0 0.0 0.0 0.0 136-137 7.4 0.0 0.0 0.0 0.0 138-139 7.9625 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content pass >>END_MODULE SRR6941561 read2 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR6941561_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 33.97375 35.0 35.0 35.0 33.0 35.0 2 34.135 35.0 35.0 35.0 33.0 35.0 3 34.34925 35.0 35.0 35.0 33.0 35.0 4 34.24925 35.0 35.0 35.0 33.0 35.0 5 34.33475 35.0 35.0 35.0 33.0 35.0 6 38.99125 40.0 40.0 40.0 38.0 40.0 7 38.84775 40.0 40.0 40.0 38.0 40.0 8 39.03825 40.0 40.0 40.0 39.0 40.0 9 39.0915 40.0 40.0 40.0 39.0 40.0 10-14 39.0322 40.0 40.0 40.0 38.6 40.0 15-19 39.15005 40.0 40.0 40.0 39.0 40.0 20-24 39.1552 40.0 40.0 40.0 38.8 40.0 25-29 39.0787 40.0 40.0 40.0 38.6 40.0 30-34 39.05595 40.0 40.0 40.0 38.6 40.0 35-39 38.9561 40.0 39.8 40.0 37.8 40.0 40-44 38.99505 40.0 40.0 40.0 38.0 40.0 45-49 38.96055 40.0 40.0 40.0 38.0 40.0 50-54 38.817150000000005 40.0 39.0 40.0 37.6 40.0 55-59 38.7089 40.0 39.2 40.0 37.0 40.0 60-64 38.654650000000004 40.0 39.0 40.0 36.6 40.0 65-69 38.72435 40.0 39.2 40.0 37.2 40.0 70-74 38.5616 40.0 39.0 40.0 36.4 40.0 75-79 38.60435 40.0 39.0 40.0 36.6 40.0 80-84 38.6253 40.0 39.0 40.0 36.6 40.0 85-89 38.782250000000005 40.0 39.0 40.0 37.2 40.0 90-94 38.691649999999996 40.0 39.0 40.0 36.8 40.0 95-99 38.43545 40.0 39.0 40.0 35.8 40.0 100-104 37.121050000000004 38.6 37.6 39.2 33.4 39.4 105-109 38.443149999999996 40.0 39.0 40.0 36.2 40.0 110-114 38.5089 40.0 39.0 40.0 36.4 40.0 115-119 38.384550000000004 40.0 39.0 40.0 35.8 40.0 120-124 38.322449999999996 40.0 39.0 40.0 35.8 40.0 125-129 38.4255 40.0 39.0 40.0 36.2 40.0 130-134 38.239599999999996 40.0 39.0 40.0 36.0 40.0 135-139 38.155199999999994 40.0 39.0 40.0 35.8 40.0 140-144 37.7348 40.0 39.0 40.0 34.6 40.0 145-149 37.4083 40.0 38.8 40.0 33.8 40.0 150-151 34.23375 38.0 34.5 39.5 24.5 40.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 0.0 4 0.0 5 1.0 6 0.0 7 0.0 8 1.0 9 0.0 10 1.0 11 1.0 12 0.0 13 1.0 14 1.0 15 2.0 16 2.0 17 0.0 18 3.0 19 2.0 20 3.0 21 5.0 22 7.0 23 12.0 24 6.0 25 11.0 26 14.0 27 22.0 28 19.0 29 27.0 30 34.0 31 30.0 32 47.0 33 44.0 34 49.0 35 100.0 36 105.0 37 164.0 38 377.0 39 2908.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 39.469026548672566 20.960809102402024 11.807838179519596 27.762326169405817 2 25.213246362267938 23.206221776216758 35.900652282990464 15.679879578524837 3 20.3 24.224999999999998 37.275000000000006 18.2 4 24.45 30.675 26.125 18.75 5 26.700000000000003 30.075000000000003 25.3 17.925 6 22.95 33.85 25.624999999999996 17.575 7 19.05 20.825 40.8 19.325 8 21.525 24.2 29.599999999999998 24.675 9 22.925 21.425 32.9 22.75 10-14 24.54 25.86 29.595 20.005 15-19 24.375 24.610000000000003 30.049999999999997 20.965 20-24 23.78 26.005 30.195 20.02 25-29 24.745 25.124999999999996 29.775000000000002 20.355 30-34 25.135 24.279999999999998 30.56 20.025000000000002 35-39 25.305 25.115 29.485 20.095 40-44 24.62 25.419999999999998 29.7 20.26 45-49 23.96 26.305 28.92 20.815 50-54 24.09 25.130000000000003 30.09 20.69 55-59 24.385 26.955000000000002 27.965 20.695 60-64 24.025 24.65 29.895 21.43 65-69 24.45 25.735000000000003 28.884999999999998 20.93 70-74 25.0 25.124999999999996 30.135 19.74 75-79 25.005 24.865000000000002 29.459999999999997 20.669999999999998 80-84 24.275 24.709999999999997 31.045 19.97 85-89 24.85 25.435000000000002 29.125 20.59 90-94 24.735 25.045 28.075 22.145 95-99 25.074999999999996 24.555 29.285 21.085 100-104 24.445 26.0 29.115000000000002 20.44 105-109 25.865 23.94 29.845 20.349999999999998 110-114 25.55 24.990000000000002 29.565 19.895 115-119 25.19 25.424999999999997 29.360000000000003 20.025000000000002 120-124 23.775 25.855 28.345 22.025 125-129 24.87 26.334999999999997 28.084999999999997 20.71 130-134 24.47 25.985000000000003 28.499999999999996 21.044999999999998 135-139 24.83 25.86 28.645 20.665 140-144 25.814999999999998 25.77 28.599999999999998 19.814999999999998 145-149 25.419999999999998 25.81 28.185 20.585 150-151 24.620784756174 24.13187915256362 31.239814466591447 20.00752162467093 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1.0 1 0.5 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.5 17 0.5 18 0.0 19 0.5 20 1.0 21 1.5 22 2.5 23 2.5 24 5.5 25 9.0 26 9.0 27 8.5 28 11.0 29 15.0 30 21.5 31 28.5 32 27.0 33 47.0 34 76.5 35 80.5 36 86.0 37 145.0 38 182.0 39 190.0 40 226.5 41 245.5 42 214.5 43 210.0 44 230.0 45 197.0 46 179.0 47 155.0 48 110.0 49 87.0 50 80.0 51 81.5 52 74.0 53 80.5 54 114.5 55 135.5 56 121.5 57 92.5 58 79.0 59 74.5 60 61.5 61 47.0 62 44.0 63 34.5 64 19.0 65 11.5 66 6.5 67 7.0 68 7.0 69 5.5 70 4.5 71 2.5 72 2.0 73 1.5 74 1.5 75 1.0 76 1.0 77 0.5 78 0.5 79 0.5 80 0.0 81 0.5 82 0.5 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 1.125 2 0.35000000000000003 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.2875 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 64.85 #Duplication Level Percentage of deduplicated Percentage of total 1 80.84040092521204 52.425 2 9.136468774094062 11.85 3 3.932151117964534 7.6499999999999995 4 1.8118735543562068 4.7 5 1.040863531225906 3.375 6 0.5397070161912104 2.1 7 0.8866615265998458 4.025 8 0.4626060138781804 2.4 9 0.30840400925212025 1.7999999999999998 >10 1.040863531225906 9.675 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source ATTATCTATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAAT 33 0.8250000000000001 No Hit TATCTATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTT 23 0.575 No Hit GTTCGATCCTGGCTCAGGATGAACGCTGGCGGCATGCTTAACACATGCAA 21 0.525 No Hit GCTTGTTATATGGGTCGTGAGTGGGAACTTAGTTTCCGTTTGGGTATGCG 18 0.44999999999999996 No Hit CTATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAA 18 0.44999999999999996 No Hit GCTAGTTTCAACAACTCTCGTTCTTTACACTTCTTCTTGGCTGCTTGGCC 16 0.4 No Hit GCACAACATCCTTATGCATCCATTTCACATGTTAGGTGTAGCTGGTGTTT 16 0.4 No Hit GTGGGAACTTAGTTTCCGTTTGGGTATGCGCCCTTGGATTGCTGTTGCAT 15 0.375 No Hit GTTAGGTGTAGCTGGTGTTTTCGGCGGTTCCCTATTCAGTGCTATGCATG 15 0.375 No Hit ATCTATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTG 15 0.375 No Hit ATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAA 14 0.35000000000000003 No Hit GGCTTGCGGTGGATACCTAGGCACCCAGAGACGAGGAAGGGCGTAGCAAG 13 0.325 No Hit GTAGGAATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCT 13 0.325 No Hit ATTCAGTGCTATGCATGGTTCCTTGGTAACCTCTAGTTTGATCAGGGAAA 13 0.325 No Hit GTTATACAATGGTGGTCCTTATGAGCTAATTGTTCTACACTTCTTACTTG 12 0.3 No Hit GTTTCAACAACTCTCGTTCTTTACACTTCTTCTTGGCTGCTTGGCCTGTA 12 0.3 No Hit CCTTACCAGGGCTTGACATGCCGCGAATCCTCTTGAAAGAGAGGGGTGCC 12 0.3 No Hit TCTATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGA 12 0.3 No Hit GTTTGATCAGGGAAACTACTGAAAATGAATCTGCTAATGAGGGTTACAAA 12 0.3 No Hit CTTCCAATATGCTAGTTTCAACAACTCTCGTTCTTTACACTTCTTCTTGG 12 0.3 No Hit GTTTTGATGATCCCTACCTTATTGACCGCAACTTCTGTATTTATTATCGC 11 0.27499999999999997 No Hit CTAGCACTGAAAATCGTCTTTACATCGGATGGTTCGGTGTTTTGATGATC 11 0.27499999999999997 No Hit AGCACAACATCCTTATGCATCCATTTCACATGTTAGGTGTAGCTGGTGTT 10 0.25 No Hit CTGGTACTTTCAACTTTATGATTGTATTCCAGGCAGAGCACAACATCCTT 10 0.25 No Hit GGAAACTACTGAAAATGAATCTGCTAATGAGGGTTACAAATTTGGTCAAG 10 0.25 No Hit ATTTGGTCAAGAGGAAGAGACTTATAATATTGTGGCTGCTCATGGTTATT 10 0.25 No Hit GTTGCATATTCAGCTCCTGTTGCAGCTGCGACTGCTGTTTTCTTGATTTA 10 0.25 No Hit GGGAAACTACTGAAAATGAATCTGCTAATGAGGGTTACAAATTTGGTCAA 9 0.22499999999999998 No Hit CCAAGATCTTACCATGACTGCAATTTTAGAGAGACGCGAAAGTACAAGCC 9 0.22499999999999998 No Hit CGCAGCCCCTCCAGTAGATATTGATGGTATTCGCGAGCCTGTTTCTGGTT 9 0.22499999999999998 No Hit GGAAGCTTCTCTGATGGTATGCCTTTAGGAATATCTGGTACTTTCAACTT 9 0.22499999999999998 No Hit CAGTGACCAGGCCCGGGCGACTGTTTACCAAAAACACAGGTCTCCGCAAA 9 0.22499999999999998 No Hit GCTGCGACTGCTGTTTTCTTGATTTACCCTATTGGTCAAGGAAGCTTCTC 9 0.22499999999999998 No Hit GAAGAGACTTATAATATTGTGGCTGCTCATGGTTATTTTGGCCGATTAAT 9 0.22499999999999998 No Hit GTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGATCTTACCATGAC 9 0.22499999999999998 No Hit GCTCATGGTTATTTTGGCCGATTAATCTTCCAATATGCTAGTTTCAACAA 8 0.2 No Hit CAACTCTCGTTCTTTACACTTCTTCTTGGCTGCTTGGCCTGTAGTAGGAA 8 0.2 No Hit CCTAAATGGATTCAATTTCAACCAATCTGTAGTTGATAGTCAAGGTCGCG 8 0.2 No Hit GTTATTTTGGCCGATTAATCTTCCAATATGCTAGTTTCAACAACTCTCGT 8 0.2 No Hit GTAGCTTGTTATATGGGTCGTGAGTGGGAACTTAGTTTCCGTTTGGGTAT 8 0.2 No Hit CTTACCATGACTGCAATTTTAGAGAGACGCGAAAGTACAAGCCTGTGGGG 8 0.2 No Hit TGTAGCTGGTGTTTTCGGCGGTTCCCTATTCAGTGCTATGCATGGTTCCT 8 0.2 No Hit CAATGGTGGTCCTTATGAGCTAATTGTTCTACACTTCTTACTTGGTGTAG 8 0.2 No Hit GAGGAAGAGACTTATAATATTGTGGCTGCTCATGGTTATTTTGGCCGATT 8 0.2 No Hit CGAAAGTACAAGCCTGTGGGGTCGCTTCTGCAACTGGATAACTAGCACTG 8 0.2 No Hit AGGGAAACTACTGAAAATGAATCTGCTAATGAGGGTTACAAATTTGGTCA 8 0.2 No Hit GGTACTTTCAACTTTATGATTGTATTCCAGGCAGAGCACAACATCCTTAT 8 0.2 No Hit GGAAGAGACTTATAATATTGTGGCTGCTCATGGTTATTTTGGCCGATTAA 7 0.17500000000000002 No Hit CTTTTACCCAATTTGGGAAGCTGCATCCGTTGATGAATGGTTATACAATG 7 0.17500000000000002 No Hit CTGCAACTGGATAACTAGCACTGAAAATCGTCTTTACATCGGATGGTTCG 7 0.17500000000000002 No Hit AAATTTGGTCAAGAGGAAGAGACTTATAATATTGTGGCTGCTCATGGTTA 7 0.17500000000000002 No Hit GTACTATGGCGTTCAACCTAAATGGATTCAATTTCAACCAATCTGTAGTT 7 0.17500000000000002 No Hit ATCGCCTTCATCGCAGCCCCTCCAGTAGATATTGATGGTATTCGCGAGCC 7 0.17500000000000002 No Hit CTTGCGGTGGATACCTAGGCACCCAGAGACGAGGAAGGGCGTAGCAAGCG 7 0.17500000000000002 No Hit GGGAATTTCCGGTGGAGCGGTGAAATGCATTGAGATCGGAAAGAACACCA 7 0.17500000000000002 No Hit CGTTGATGAATGGTTATACAATGGTGGTCCTTATGAGCTAATTGTTCTAC 7 0.17500000000000002 No Hit GAACGCTGGCGGCATGCTTAACACATGCAAGTCGAACGGGAAGTGGTGTT 7 0.17500000000000002 No Hit TTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAG 7 0.17500000000000002 No Hit CCTGCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGCAA 7 0.17500000000000002 No Hit TGAACGCTGGCGGCATGCTTAACACATGCAAGTCGAACGGGAAGTGGTGT 7 0.17500000000000002 No Hit ATTCAGCTCCTGTTGCAGCTGCGACTGCTGTTTTCTTGATTTACCCTATT 7 0.17500000000000002 No Hit ATTGTATTCCAGGCAGAGCACAACATCCTTATGCATCCATTTCACATGTT 7 0.17500000000000002 No Hit CAGGGAAACTACTGAAAATGAATCTGCTAATGAGGGTTACAAATTTGGTC 7 0.17500000000000002 No Hit GGCCGATTAATCTTCCAATATGCTAGTTTCAACAACTCTCGTTCTTTACA 7 0.17500000000000002 No Hit GTTCAACCTAAATGGATTCAATTTCAACCAATCTGTAGTTGATAGTCAAG 7 0.17500000000000002 No Hit GTACAAGCCTGTGGGGTCGCTTCTGCAACTGGATAACTAGCACTGAAAAT 7 0.17500000000000002 No Hit CTCATGGTTATTTTGGCCGATTAATCTTCCAATATGCTAGTTTCAACAAC 7 0.17500000000000002 No Hit CTTTATGATTGTATTCCAGGCAGAGCACAACATCCTTATGCATCCATTTC 7 0.17500000000000002 No Hit CTGAAAATGAATCTGCTAATGAGGGTTACAAATTTGGTCAAGAGGAAGAG 7 0.17500000000000002 No Hit GTTCTTTACTTTATGGAAACAATATTATCTCTGGTGCTATTATTCCTACT 7 0.17500000000000002 No Hit CATGGTTATTTTGGCCGATTAATCTTCCAATATGCTAGTTTCAACAACTC 6 0.15 No Hit ATTATTCCTACTTCTGCGGCAATCGGATTGCACTTTTACCCAATTTGGGA 6 0.15 No Hit ATCGGATTGCACTTTTACCCAATTTGGGAAGCTGCATCCGTTGATGAATG 6 0.15 No Hit GAATCTGCTAATGAGGGTTACAAATTTGGTCAAGAGGAAGAGACTTATAA 6 0.15 No Hit CTCTAGTTTGATCAGGGAAACTACTGAAAATGAATCTGCTAATGAGGGTT 6 0.15 No Hit CTGCTAATGAGGGTTACAAATTTGGTCAAGAGGAAGAGACTTATAATATT 6 0.15 No Hit CATGACTGCAATTTTAGAGAGACGCGAAAGTACAAGCCTGTGGGGTCGCT 6 0.15 No Hit CTTGTTATATGGGTCGTGAGTGGGAACTTAGTTTCCGTTTGGGTATGCGC 6 0.15 No Hit CTTAGTTTCCGTTTGGGTATGCGCCCTTGGATTGCTGTTGCATATTCAGC 6 0.15 No Hit TTCCAATATGCTAGTTTCAACAACTCTCGTTCTTTACACTTCTTCTTGGC 6 0.15 No Hit ACCTTACCAGGGCTTGACATGCCGCGAATCCTCTTGAAAGAGAGGGGTGC 6 0.15 No Hit AACATCCTTATGCATCCATTTCACATGTTAGGTGTAGCTGGTGTTTTCGG 6 0.15 No Hit CGGGTGAGTAACGCGTAAGAACCTGCCCTTGGGAGGGGAACAACAACTGG 6 0.15 No Hit CAACATCCTTATGCATCCATTTCACATGTTAGGTGTAGCTGGTGTTTTCG 6 0.15 No Hit GTTTTCGGCGGTTCCCTATTCAGTGCTATGCATGGTTCCTTGGTAACCTC 5 0.125 No Hit GTCGTGAGTGGGAACTTAGTTTCCGTTTGGGTATGCGCCCTTGGATTGCT 5 0.125 No Hit GAACCTTACCAGGGCTTGACATGCCGCGAATCCTCTTGAAAGAGAGGGGT 5 0.125 No Hit GTCTTTACATCGGATGGTTCGGTGTTTTGATGATCCCTACCTTATTGACC 5 0.125 No Hit GTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGATCTT 5 0.125 No Hit TAGGAATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCTA 5 0.125 No Hit GTTCCTTGGTAACCTCTAGTTTGATCAGGGAAACTACTGAAAATGAATCT 5 0.125 No Hit CTTCTGTATTTATTATCGCCTTCATCGCAGCCCCTCCAGTAGATATTGAT 5 0.125 No Hit CTTCTTCTTGGCTGCTTGGCCTGTAGTAGGAATCTGGTTCACTGCTTTAG 5 0.125 No Hit CTGAGGAATAAGCATCGGCTAACTCTGTGCCAGCAGCCGCGGTAAGACAG 5 0.125 No Hit GCTAATGAGGGTTACAAATTTGGTCAAGAGGAAGAGACTTATAATATTGT 5 0.125 No Hit CTTTACTTTATGGAAACAATATTATCTCTGGTGCTATTATTCCTACTTCT 5 0.125 No Hit ATCTGGTACTTTCAACTTTATGATTGTATTCCAGGCAGAGCACAACATCC 5 0.125 No Hit AATGAATCTGCTAATGAGGGTTACAAATTTGGTCAAGAGGAAGAGACTTA 5 0.125 No Hit GTCGAACGGGAAGTGGTGTTTCCAGTGGCGAACGGGTGAGTAACGCGTAA 5 0.125 No Hit GGAAGGCCTACGGGTCGTCAACTTCTTTTCTCGGAGAAGAAACAATGACG 5 0.125 No Hit CTTGCAATTTGAATAAACCAAGATCTTACCATGACTGCAATTTTAGAGAG 5 0.125 No Hit AAACAATATTATCTCTGGTGCTATTATTCCTACTTCTGCGGCAATCGGAT 5 0.125 No Hit GCATGGTTCCTTGGTAACCTCTAGTTTGATCAGGGAAACTACTGAAAATG 5 0.125 No Hit ATTATCTCTGGTGCTATTATTCCTACTTCTGCGGCAATCGGATTGCACTT 5 0.125 No Hit GGAATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCTAAA 5 0.125 No Hit GCCTTTAGGAATATCTGGTACTTTCAACTTTATGATTGTATTCCAGGCAG 5 0.125 No Hit GTTGGGTTAAGTCTCGCAACGAGCGCAACCCTCGTGTTTAGTTGCCACTA 5 0.125 No Hit CCTAGGCACCCAGAGACGAGGAAGGGCGTAGCAAGCGACGAAATGCTTCG 5 0.125 No Hit GGATAACTAGCACTGAAAATCGTCTTTACATCGGATGGTTCGGTGTTTTG 5 0.125 No Hit AATGCATTGAGATCGGAAAGAACACCAACGGCGAAAGCACTCTGCTGGGC 5 0.125 No Hit CGGCAATCGGATTGCACTTTTACCCAATTTGGGAAGCTGCATCCGTTGAT 5 0.125 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.025 0.0 0.0 0.0 0.0 62-63 0.025 0.0 0.0 0.0 0.0 64-65 0.025 0.0 0.0 0.0 0.0 66-67 0.025 0.0 0.0 0.0 0.0 68-69 0.05 0.0 0.0 0.0 0.0 70-71 0.05 0.0 0.0 0.0 0.0 72-73 0.075 0.0 0.0 0.0 0.0 74-75 0.075 0.0 0.0 0.0 0.0 76-77 0.125 0.0 0.0 0.0 0.0 78-79 0.15 0.0 0.0 0.0 0.0 80-81 0.15 0.0 0.0 0.0 0.0 82-83 0.2 0.0 0.0 0.0 0.0 84-85 0.3 0.0 0.0 0.0 0.0 86-87 0.4125 0.0 0.0 0.0 0.0 88-89 0.575 0.0 0.0 0.0 0.0 90-91 0.6625000000000001 0.0 0.0 0.0 0.0 92-93 0.8125 0.0 0.0 0.0 0.0 94-95 0.975 0.0 0.0 0.0 0.0 96-97 1.05 0.0 0.0 0.0 0.0 98-99 1.125 0.0 0.0 0.0 0.0 100-101 1.25 0.0 0.0 0.0 0.0 102-103 1.4 0.0 0.0 0.0 0.0 104-105 1.6 0.0 0.0 0.0 0.0 106-107 1.8625 0.0 0.0 0.0 0.0 108-109 2.1125 0.0 0.0 0.0 0.0 110-111 2.5375 0.0 0.0 0.0 0.0 112-113 2.875 0.0 0.0 0.0 0.0 114-115 3.25 0.0 0.0 0.0 0.0 116-117 3.525 0.0 0.0 0.0 0.0 118-119 3.8 0.0 0.0 0.0 0.0 120-121 4.050000000000001 0.0 0.0 0.0 0.0 122-123 4.4625 0.0 0.0 0.0 0.0 124-125 4.8375 0.0 0.0 0.0 0.0 126-127 5.3 0.0 0.0 0.0 0.0 128-129 5.725 0.0 0.0 0.0 0.0 130-131 6.012499999999999 0.0 0.0 0.0 0.0 132-133 6.425 0.0 0.0 0.0 0.0 134-135 6.875 0.0 0.0 0.0 0.0 136-137 7.35 0.0 0.0 0.0 0.0 138-139 7.9625 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content pass >>END_MODULE Read 1137239 spots for SRR6941561.sra Written 1137239 spots for SRR6941561.sra Read 1137239 spots for SRR6941561.sra Written 1137239 spots for SRR6941561.sra Read 1137239 spots for SRR6941561.sra Written 1137239 spots for SRR6941561.sra Read 1137239 spots for SRR6941561.sra Written 1137239 spots for SRR6941561.sra Read 1137239 spots for SRR6941561.sra Written 1137239 spots for SRR6941561.sra Read 1137239 spots for SRR6941561.sra Written 1137239 spots for SRR6941561.sra Read 1137239 spots for SRR6941561.sra Written 1137239 spots for SRR6941561.sra Read 1137239 spots for SRR6941561.sra Written 1137239 spots for SRR6941561.sra Read 1137239 spots for SRR6941561.sra Written 1137239 spots for SRR6941561.sra Read 1137239 spots for SRR6941561.sra Written 1137239 spots for SRR6941561.sra Read 1137239 spots for SRR6941561.sra Written 1137239 spots for SRR6941561.sra Read 1137239 spots for SRR6941561.sra Written 1137239 spots for SRR6941561.sra Read 1137239 spots for SRR6941561.sra Written 1137239 spots for SRR6941561.sra Read 1137253 spots for SRR6941561.sra Written 1137253 spots for SRR6941561.sra Read 1137239 spots for SRR6941561.sra Written 1137239 spots for SRR6941561.sra Read 1137239 spots for SRR6941561.sra Written 1137239 spots for SRR6941561.sra Read 1137239 spots for SRR6941561.sra Written 1137239 spots for SRR6941561.sra Read 1137239 spots for SRR6941561.sra Written 1137239 spots for SRR6941561.sra Read 1137239 spots for SRR6941561.sra Written 1137239 spots for SRR6941561.sra Read 1137239 spots for SRR6941561.sra Written 1137239 spots for SRR6941561.sra SRR ids: ['SRR6941561.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_klc7ztjn SRR6941561.sra spots: 22744794 blocks: [[1, 1137239], [1137240, 2274478], [2274479, 3411717], [3411718, 4548956], [4548957, 5686195], [5686196, 6823434], [6823435, 7960673], [7960674, 9097912], [9097913, 10235151], [10235152, 11372390], [11372391, 12509629], [12509630, 13646868], [13646869, 14784107], [14784108, 15921346], [15921347, 17058585], [17058586, 18195824], [18195825, 19333063], [19333064, 20470302], [20470303, 21607541], [21607542, 22744794]] SRR6941561 file size 7685763 SRR6941561 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR6941561 SRR6941561_1.fastq SRR6941561_2.fastq Input file: SRR6941561_1.fastq Paired file: SRR6941561_2.fastq trimmed: SRR6941561-trimmed-pair1.fastq, SRR6941561-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Fri Dec 6 11:23:30 2024 >> started Fri Dec 6 11:23:58 2024 >> done (27.615s) 22744794 read pairs processed; of these: 6309 ( 0.03%) short read pairs filtered out after trimming by size control 8153 ( 0.04%) empty read pairs filtered out after trimming by size control 22730332 (99.94%) read pairs available; of these: 4418649 (19.44%) trimmed read pairs available after processing 18311683 (80.56%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 19 3 0.00% 20 3 0.00% 21 2 0.00% 22 2 0.00% 23 1 0.00% 24 1 0.00% 25 4 0.00% 26 2 0.00% 27 5 0.00% 28 3 0.00% 29 12 0.00% 30 15 0.00% 31 19 0.00% 32 19 0.00% 33 11 0.00% 34 19 0.00% 35 14 0.00% 36 24 0.00% 37 31 0.00% 38 39 0.00% 39 48 0.00% 40 46 0.00% 41 56 0.00% 42 57 0.00% 43 53 0.00% 44 77 0.00% 45 85 0.00% 46 86 0.00% 47 102 0.00% 48 108 0.00% 49 114 0.00% 50 129 0.00% 51 165 0.00% 52 183 0.00% 53 209 0.00% 54 297 0.00% 55 295 0.00% 56 285 0.00% 57 341 0.00% 58 408 0.00% 59 461 0.00% 60 560 0.00% 61 631 0.00% 62 808 0.00% 63 857 0.00% 64 993 0.00% 65 1151 0.01% 66 1161 0.01% 67 1243 0.01% 68 1621 0.01% 69 1847 0.01% 70 2114 0.01% 71 2506 0.01% 72 2981 0.01% 73 3258 0.01% 74 3129 0.01% 75 3784 0.02% 76 3656 0.02% 77 4331 0.02% 78 4545 0.02% 79 4964 0.02% 80 5668 0.02% 81 6229 0.03% 82 7351 0.03% 83 7530 0.03% 84 8220 0.04% 85 9924 0.04% 86 10462 0.05% 87 11153 0.05% 88 12548 0.06% 89 12529 0.06% 90 14068 0.06% 91 14498 0.06% 92 17110 0.08% 93 17273 0.08% 94 18412 0.08% 95 20145 0.09% 96 18612 0.08% 97 19720 0.09% 98 19676 0.09% 99 20996 0.09% 100 21587 0.09% 101 23487 0.10% 102 25561 0.11% 103 25740 0.11% 104 28123 0.12% 105 29300 0.13% 106 30050 0.13% 107 30301 0.13% 108 30102 0.13% 109 34714 0.15% 110 32470 0.14% 111 36552 0.16% 112 38177 0.17% 113 34666 0.15% 114 37870 0.17% 115 35225 0.15% 116 36702 0.16% 117 35172 0.15% 118 35334 0.16% 119 35520 0.16% 120 37485 0.16% 121 37382 0.16% 122 41855 0.18% 123 44246 0.19% 124 45214 0.20% 125 47067 0.21% 126 42393 0.19% 127 42773 0.19% 128 44428 0.20% 129 49794 0.22% 130 48881 0.22% 131 53294 0.23% 132 53785 0.24% 133 48036 0.21% 134 55455 0.24% 135 52437 0.23% 136 56247 0.25% 137 54947 0.24% 138 60732 0.27% 139 63223 0.28% 140 60272 0.27% 141 72482 0.32% 142 64676 0.28% 143 70498 0.31% 144 69258 0.30% 145 80974 0.36% 146 86726 0.38% 147 98172 0.43% 148 126207 0.56% 149 195648 0.86% 150 1555311 6.84% 151 18311683 80.56% 22730332 reads passed initial QC criterion=sequence-density sequence-density=0.33 sequence-density-rank=1 fanout-score=1.96 fanout-score-rank=30 prefix-density=0.32 prefix-fanout=2.0 sequence=CTAGAATTACTACGGTTATCCGAGTAGCACGTACCATCAAACAAACTATAACTGATTTAATGAGCCATTCGCAGTTTCACAGTTCAAATTAGTTCATACTTGCACATGCATGGCTTAATCTTTGAGACAAGCATATGACTACTGGCAGG criterion=fanout-score sequence-density=0.01 sequence-density-rank=39 fanout-score=66.91 fanout-score-rank=1 prefix-density=0.25 prefix-fanout=1.6 sequence=AGCACTCATCTTGGGGTGGGCTTACTACTTATATGCTTTCAGCAGTTATCCTCTCCGCACTTGGCTACCCAGCGTTTACCGTAGGCACGATAACTGGTACACCAGAGGTGCGTCCTTCCCGGTCCTCTCGTACTAGGGAAAGGTCCTCTCAATGCTCTAACGCCCACACCGGATATGGACCGAACTGTCTCACGACGTTCTGAACCCAGCTCACGTACCGCATTAATGGGCGAACAGCCCAACCCTTGGAACCACCTACAGCTCCAGGTGGCGAAGAGCCGACATCGAGGTGCCAAACCTTCCCGTCGATGTGGACTCTTGGGGAAGATCAGCCTGTTATCCCTAGAGTAACTTTTATCCGTTGAGCGACGGCCCTTCCACTCGGCACCGTCGGATCACTAAGGCCGACTTTCGTCTCTGCTCGACGGGTGAGTCTTGCAGTCAAGCTCCCTTCTGCCTTTGCACTCGAGGACCAATGTCCGTCTGGCCCGAGGAAACCTTTGCACGCCTC criterion=sequence-density sequence-density=0.26 sequence-density-rank=1 fanout-score=2.01 fanout-score-rank=24 prefix-density=0.26 prefix-fanout=2.0 sequence=GCGTGAGGCTGG criterion=fanout-score sequence-density=0.01 sequence-density-rank=41 fanout-score=599.71 fanout-score-rank=1 prefix-density=3.09 prefix-fanout=1.0 sequence=AAGTAATGCAACTATGAATCTCATGGAGAGTTCGATCCTGGCTCAGGATGAACGCTGGCGGCATGCTTAACACATGCAAGTCGAACGGGAAGTGGTGTTTCCAGTGGCGAACGGGTGAGTAACGCGTAAGAACCTGCCCTTGGGAGGGGAACAACAACTGGAAACGGTTGCTAATACCCCGTAGGCTGAGGAGCAAAAGGAGAAATCCGCCCAAGGAGGGGCTCGCGTCTGATTAGCTAGTTGGTGAGGCAATAGCTTACCAAGGCGATGATCAGTAGCTGGTCCGAGAGGATGATCAGCCACACTGGGACTGAGACACGGCCCAGACTCCTACGGGAGGCAGCAGTGGGGAATTTTCCGCAATGGGCGAAAGCCTGACGGAGCAATGCCGCGTGGAGGTGGAAGGCCTACGGGTCGTCAACTTCTTTTCTCGGAGAAGAAACAATGACGGTATCTGAGGAATAAGCATCGGCTAACTCTGTGCCAGCAGCCGCGGTAAGACAGAGGATGC SRR6941561 testing PE reads STAR mapping to Ensembl genome Started job on | Dec 06 11:24:54 Started mapping on | Dec 06 11:24:55 Finished on | Dec 06 11:26:54 Mapping speed, Million of reads per hour | 687.64 Number of input reads | 22730332 Average input read length | 295 UNIQUE READS: Uniquely mapped reads number | 13339052 Uniquely mapped reads % | 58.68% Average mapped length | 296.68 Number of splices: Total | 2420287 Number of splices: Annotated (sjdb) | 2157989 Number of splices: GT/AG | 2263943 Number of splices: GC/AG | 29107 Number of splices: AT/AC | 12135 Number of splices: Non-canonical | 115102 Mismatch rate per base, % | 0.25% Deletion rate per base | 0.02% Deletion average length | 1.93 Insertion rate per base | 0.02% Insertion average length | 2.62 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 7372509 % of reads mapped to multiple loci | 32.43% Number of reads mapped to too many loci | 143998 % of reads mapped to too many loci | 0.63% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 5.08% % of reads unmapped: other | 3.17% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 2024537 2024537 2024537 N_multimapping 7372509 7372509 7372509 N_noFeature 6391238 12869014 6619880 N_ambiguous 466727 4549 233051 UnstrandedReadsAssigned:6481087 PositiveStrandReadsAssigned:465489 NegativeStrandReadsAssigned:6486121 Dataset is classified negative stranded MeadianReadLen=151 20thPercentileLength=151 echo kmer=147 SRR6941561 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,972 [index] number of k-mers: 66,720,672 [index] number of equivalence classes: 111,837 [quant] running in paired-end mode [quant] will process pair 1: SRR6941561-trimmed-pair1.fastq SRR6941561-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 22,730,332 reads, 10,854,890 reads pseudoaligned [quant] estimated average fragment length: 247.291 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,091 rounds 52973 SRR6941561.ke.tsv 35125 SRR6941561.se.tsv 88098 total ==> SRR6941561.ke.tsv <== target_id length eff_length est_counts tpm PNS24245 936 690.026 0 0 PNS24247 1044 797.709 15.0072 1.33803 PNS24249 1928 1681.71 2.69433 0.113949 PNS24246 1044 797.709 15.0072 1.33803 PNS24248 1044 797.709 15.0072 1.33803 PNS24244 1471 1224.71 51.284 2.97824 PNS24243 293 98.5689 1 0.721557 KQK14069 1603 1356.71 1622.62 85.0627 KQK14071 474 240.282 26.8955 7.96099 ==> SRR6941561.se.tsv <== BRADI_1g14170v3 2416 BRADI_1g53295v3 50 BRADI_1g59795v3 73 BRADI_1g07683v3 0 BRADI_1g00485v3 0 BRADI_1g20270v3 60 BRADI_1g74790v3 2 BRADI_1g09890v3 0 BRADI_1g77505v3 66 BRADI_1g48960v3 0 SRR6941561 completed mapping pipeline successfully