Starting /dee2/code/volunteer_pipeline.sh SRR6941562 current disk space = 1551253000192 free memory = 1600754180 SRR6941562 SRAfilesize daf1d4e06fd02f298e18b1059b978c26 SRR6941562.sra SRR6941562.sra file validated SRR6941562 is paired end SRR6941562 is conventional basespace SRR6941562 read1 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR6941562_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 34.71875 35.0 35.0 35.0 35.0 35.0 2 34.62625 35.0 35.0 35.0 35.0 35.0 3 34.6825 35.0 35.0 35.0 35.0 35.0 4 34.719 35.0 35.0 35.0 35.0 35.0 5 34.67625 35.0 35.0 35.0 35.0 35.0 6 39.51275 40.0 40.0 40.0 39.0 40.0 7 39.51075 40.0 40.0 40.0 39.0 40.0 8 39.45775 40.0 40.0 40.0 39.0 40.0 9 39.52625 40.0 40.0 40.0 39.0 40.0 10-14 39.4601 40.0 40.0 40.0 39.0 40.0 15-19 39.409400000000005 40.0 40.0 40.0 39.0 40.0 20-24 39.4281 40.0 40.0 40.0 39.0 40.0 25-29 39.42085 40.0 40.0 40.0 39.0 40.0 30-34 39.27385 40.0 40.0 40.0 39.0 40.0 35-39 39.4411 40.0 40.0 40.0 39.0 40.0 40-44 39.38275 40.0 40.0 40.0 39.0 40.0 45-49 39.37265 40.0 40.0 40.0 39.0 40.0 50-54 39.28485 40.0 40.0 40.0 38.8 40.0 55-59 39.288500000000006 40.0 40.0 40.0 39.0 40.0 60-64 39.29105 40.0 40.0 40.0 39.0 40.0 65-69 39.2744 40.0 40.0 40.0 39.0 40.0 70-74 39.2251 40.0 40.0 40.0 38.8 40.0 75-79 39.122 40.0 40.0 40.0 38.6 40.0 80-84 39.192600000000006 40.0 40.0 40.0 38.6 40.0 85-89 39.158899999999996 40.0 40.0 40.0 38.6 40.0 90-94 39.08345 40.0 40.0 40.0 38.6 40.0 95-99 39.1275 40.0 40.0 40.0 38.4 40.0 100-104 38.238749999999996 39.2 38.6 39.4 36.8 39.8 105-109 39.050200000000004 40.0 39.6 40.0 38.2 40.0 110-114 39.0805 40.0 40.0 40.0 38.6 40.0 115-119 39.1558 40.0 40.0 40.0 38.8 40.0 120-124 38.94225 40.0 39.8 40.0 37.8 40.0 125-129 38.85985 40.0 39.2 40.0 37.6 40.0 130-134 38.8274 40.0 39.0 40.0 37.4 40.0 135-139 38.6823 40.0 39.0 40.0 36.6 40.0 140-144 38.481199999999994 40.0 39.0 40.0 36.2 40.0 145-149 38.5231 40.0 39.0 40.0 36.8 40.0 150-151 36.714749999999995 39.5 37.0 40.0 31.5 40.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 20 1.0 21 0.0 22 0.0 23 1.0 24 6.0 25 3.0 26 8.0 27 9.0 28 7.0 29 22.0 30 20.0 31 33.0 32 37.0 33 34.0 34 45.0 35 64.0 36 85.0 37 131.0 38 295.0 39 3199.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 58.70870870870871 10.335335335335335 4.52952952952953 26.426426426426424 2 24.425 11.85 29.625 34.1 3 23.625 18.525 25.650000000000002 32.2 4 27.05 26.424999999999997 21.224999999999998 25.3 5 24.912368552829246 34.101151727591386 22.508763144717076 18.477716574862292 6 20.200000000000003 36.825 21.825 21.15 7 13.950000000000001 29.925 39.175 16.950000000000003 8 17.675 26.700000000000003 30.225 25.4 9 17.4 24.275 34.55 23.775 10-14 20.544999999999998 32.05 25.3 22.105 15-19 21.13 29.709999999999997 25.275 23.885 20-24 19.209802450612653 30.462615653913478 26.39659914978745 23.93098274568642 25-29 22.375 30.2 26.165 21.26 30-34 22.264999999999997 31.795 24.295 21.645 35-39 22.33 29.360000000000003 25.96 22.35 40-44 19.439999999999998 29.57 26.534999999999997 24.455 45-49 20.52 29.310000000000002 27.52 22.650000000000002 50-54 21.89 29.68 25.435000000000002 22.994999999999997 55-59 20.9 29.509999999999998 25.465 24.125 60-64 19.515 29.92 27.095000000000002 23.47 65-69 20.145 30.5 25.580000000000002 23.775 70-74 20.599999999999998 29.904999999999998 24.315 25.180000000000003 75-79 21.005 28.575 26.200000000000003 24.22 80-84 22.475 29.360000000000003 24.990000000000002 23.175 85-89 21.955 29.160000000000004 25.669999999999998 23.215 90-94 19.645000000000003 30.195 25.795 24.365000000000002 95-99 20.369999999999997 30.669999999999998 23.735 25.224999999999998 100-104 20.895 30.91 24.25 23.945 105-109 20.755000000000003 29.92 25.435000000000002 23.89 110-114 21.83 28.535 24.985 24.65 115-119 20.86 30.975 23.400000000000002 24.765 120-124 21.107941750487917 30.10058549767302 23.274783566031125 25.51668918580794 125-129 20.268040206030904 30.26453968095214 24.963744561684255 24.503675551332698 130-134 21.745 30.7 22.745 24.81 135-139 21.88 29.959999999999997 24.779999999999998 23.380000000000003 140-144 22.81 29.28 24.85 23.06 145-149 20.95 30.7 24.474999999999998 23.875 150-151 20.600750938673343 30.851063829787233 23.078848560700877 25.46933667083855 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.5 4 1.0 5 0.5 6 1.0 7 1.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.5 21 1.5 22 1.5 23 1.5 24 2.0 25 4.5 26 6.0 27 4.0 28 6.5 29 11.5 30 13.5 31 16.5 32 25.0 33 38.0 34 42.5 35 43.0 36 84.0 37 177.5 38 236.5 39 249.0 40 269.0 41 283.0 42 230.0 43 203.0 44 197.0 45 191.5 46 180.0 47 141.0 48 132.0 49 103.0 50 91.5 51 91.5 52 75.5 53 73.5 54 99.5 55 115.5 56 98.0 57 81.0 58 81.0 59 72.0 60 58.0 61 45.0 62 26.0 63 16.5 64 19.0 65 17.0 66 11.5 67 6.5 68 1.5 69 2.0 70 4.5 71 4.0 72 2.0 73 3.0 74 2.0 75 1.0 76 1.5 77 1.0 78 0.5 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1 2 0.0 3 0.0 4 0.0 5 0.15 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.025 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.08499999999999999 125-129 0.015 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.125 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.5 #Duplication Level Percentage of deduplicated Percentage of total 1 83.130081300813 51.125 2 8.130081300813007 10.0 3 3.2926829268292686 6.075 4 1.1788617886178863 2.9000000000000004 5 1.097560975609756 3.375 6 0.7317073170731708 2.7 7 0.24390243902439024 1.05 8 0.3252032520325203 1.6 9 0.2845528455284553 1.575 >10 1.5447154471544715 17.9 >50 0.04065040650406504 1.7000000000000002 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GCGGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTACG 68 1.7000000000000002 No Hit GGCCAAAATAACCATGAGCAGCCACAATATTATAAGTCTCTTCCTCTTGA 44 1.0999999999999999 No Hit GGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTACGTT 44 1.0999999999999999 No Hit GTGCTCTGCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTCCTA 36 0.8999999999999999 No Hit GCGACCTTGACTATCAACTACAGATTGGTTGAAATTGAATCCATTTAGGT 31 0.775 No Hit GCCAAAATAACCATGAGCAGCCACAATATTATAAGTCTCTTCCTCTTGAC 31 0.775 No Hit GCGGAAAAACCTTATCCATTAATAGCGGGAACTTCAAGAGCAGCTAGATC 28 0.7000000000000001 No Hit GCTCTGCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTCCTAAA 28 0.7000000000000001 No Hit GGATGCATAAGGATGTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAA 24 0.6 No Hit GTTGAAACTAGCATATTGGAAGATTAATCGGCCAAAATAACCATGAGCAG 24 0.6 No Hit GTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTC 23 0.575 No Hit GCTTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCA 22 0.5499999999999999 No Hit GTTAGCGGAAAAACCTTATCCATTAATAGCGGGAACTTCAAGAGCAGCTA 21 0.525 No Hit CTTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCAT 21 0.525 No Hit GGTCAATAAGGTAGGGATCATCAAAACACCGAACCATCCGATGTAAAGAC 19 0.475 No Hit CTCTGCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTCCTAAAG 18 0.44999999999999996 No Hit CTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCATTAAT 17 0.42500000000000004 No Hit GTGAGCATTACGTTCGTGCATTACTTCCATACCAAGATTAGCACGGTTGA 17 0.42500000000000004 No Hit GTTGAAATTGAATCCATTTAGGTTGAACGCCATAGTACTAATACCTAAAG 17 0.42500000000000004 No Hit GCATAAGGATGTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAAAGTA 16 0.4 No Hit CGGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTACGT 15 0.375 No Hit GCACGGTTGATGATATCAGCCCAAGTATTAATAACGCGACCTTGACTATC 15 0.375 No Hit GCCGAAAACACCAGCTACACCTAACATGTGAAATGGATGCATAAGGATGT 15 0.375 No Hit CCCAAGTATTAATAACGCGACCTTGACTATCAACTACAGATTGGTTGAAA 14 0.35000000000000003 No Hit CCACAATATTATAAGTCTCTTCCTCTTGACCAAATTTGTAACCCTCATTA 14 0.35000000000000003 No Hit GCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTCCTAAAGGCAT 14 0.35000000000000003 No Hit GTCGCAGCTGCAACAGGAGCTGAATATGCAACAGCAATCCAAGGGCGCAT 13 0.325 No Hit GGCGATAATAAATACAGAAGTTGCGGTCAATAAGGTAGGGATCATCAAAA 13 0.325 No Hit GTTGATGATATCAGCCCAAGTATTAATAACGCGACCTTGACTATCAACTA 13 0.325 No Hit GGCGCATACCCAAACGGAAACTAAGTTCCCACTCACGACCCATATAACAA 12 0.3 No Hit GCTAGTTATCCAGTTGCAGAAGCGACCCCACAGGCTTGTACTTTCGCGTC 12 0.3 No Hit GGTAAATCAAGAAAACAGCAGTCGCAGCTGCAACAGGAGCTGAATATGCA 12 0.3 No Hit GGAGGGGCTGCGATGAAGGCGATAATAAATACAGAAGTTGCGGTCAATAA 11 0.27499999999999997 No Hit GGAAAAACCTTATCCATTAATAGCGGGAACTTCAAGAGCAGCTAGATCTA 11 0.27499999999999997 No Hit CGGAAAAACCTTATCCATTAATAGCGGGAACTTCAAGAGCAGCTAGATCT 11 0.27499999999999997 No Hit GCTGCGATGAAGGCGATAATAAATACAGAAGTTGCGGTCAATAAGGTAGG 10 0.25 No Hit GGCCAAGCAGCCAAGAAGAAGTGTAAAGAACGAGAGTTGTTGAAACTAGC 10 0.25 No Hit GGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTACGTTC 10 0.25 No Hit CGCGACCTTGACTATCAACTACAGATTGGTTGAAATTGAATCCATTTAGG 10 0.25 No Hit GCGGTCAATAAGGTAGGGATCATCAAAACACCGAACCATCCGATGTAAAG 9 0.22499999999999998 No Hit CGAGAGTTGTTGAAACTAGCATATTGGAAGATTAATCGGCCAAAATAACC 9 0.22499999999999998 No Hit TTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCATT 9 0.22499999999999998 No Hit GGGAAGTTGTGAGCATTACGTTCGTGCATTACTTCCATACCAAGATTAGC 9 0.22499999999999998 No Hit CCTCATTAGCAGATTCATTTTCAGTAGTTTCCCTGATCAAACTAGAGGTT 9 0.22499999999999998 No Hit GGGGCTGCGATGAAGGCGATAATAAATACAGAAGTTGCGGTCAATAAGGT 9 0.22499999999999998 No Hit GCCAAGAAGAAGTGTAAAGAACGAGAGTTGTTGAAACTAGCATATTGGAA 9 0.22499999999999998 No Hit GGTTGATGATATCAGCCCAAGTATTAATAACGCGACCTTGACTATCAACT 8 0.2 No Hit GGCATGATGACTTGGCCTCATCCTCTCCTTCCTCCGGCTTAACACCGGCG 8 0.2 No Hit CATATAACAAGCTACACCAAGTAAGAAGTGTAGAACAATTAGCTCATAAG 8 0.2 No Hit GGATGTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAAAGTACCAGAT 8 0.2 No Hit CTCATAAGGACCACCATTGTATAACCATTCATCAACGGATGCAGCTTCCC 8 0.2 No Hit AGCGGAAAAACCTTATCCATTAATAGCGGGAACTTCAAGAGCAGCTAGAT 8 0.2 No Hit GATGCATAAGGATGTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAAA 8 0.2 No Hit GAATAATAGCACCAGAGATAATATTGTTTCCATAAAGTAAAGAACCAGAA 8 0.2 No Hit ACGCGACCTTGACTATCAACTACAGATTGGTTGAAATTGAATCCATTTAG 7 0.17500000000000002 No Hit GTACAAGGCCCGGGAACGGATTCACCGCCGTATGGCTGACCGGCGATTAC 7 0.17500000000000002 No Hit ATCAATATCTACTGGAGGGGCTGCGATGAAGGCGATAATAAATACAGAAG 7 0.17500000000000002 No Hit GCTAGATCTAGAGGGAAGTTGTGAGCATTACGTTCGTGCATTACTTCCAT 7 0.17500000000000002 No Hit GACCCATATAACAAGCTACACCAAGTAAGAAGTGTAGAACAATTAGCTCA 7 0.17500000000000002 No Hit GTCAATAAGGTAGGGATCATCAAAACACCGAACCATCCGATGTAAAGACG 7 0.17500000000000002 No Hit CGCAGAAGTAGGAATAATAGCACCAGAGATAATATTGTTTCCATAAAGTA 6 0.15 No Hit ACCAGATATTCCTAAAGGCATACCATCAGAGAAGCTTCCTTGACCAATAG 6 0.15 No Hit GGTAAAAGTGCAATCCGATTGCCGCAGAAGTAGGAATAATAGCACCAGAG 6 0.15 No Hit TTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCATTA 6 0.15 No Hit GCATATTGGAAGATTAATCGGCCAAAATAACCATGAGCAGCCACAATATT 6 0.15 No Hit CTAACATGTGAAATGGATGCATAAGGATGTTGTGCTCTGCCTGGAATACA 6 0.15 No Hit GAGTTGTTGAAACTAGCATATTGGAAGATTAATCGGCCAAAATAACCATG 6 0.15 No Hit GGCTGCGATGAAGGCGATAATAAATACAGAAGTTGCGGTCAATAAGGTAG 6 0.15 No Hit GTAAAGAACGAGAGTTGTTGAAACTAGCATATTGGAAGATTAATCGGCCA 6 0.15 No Hit GTTCGCTGGAGTAGGCTGCGAGGCCCAAGCGGCCAAGGAGTGCAGCGGCC 6 0.15 No Hit ACCAGAGATAATATTGTTTCCATAAAGTAAAGAACCAGAAACAGGCTCGC 6 0.15 No Hit CTCATTAGCAGATTCATTTTCAGTAGTTTCCCTGATCAAACTAGAGGTTA 6 0.15 No Hit CTCTTGACCAAATTTGTAACCCTCATTAGCAGATTCATTTTCAGTAGTTT 6 0.15 No Hit GTGAAATGGATGCATAAGGATGTTGTGCTCTGCCTGGAATACAATCATAA 6 0.15 No Hit GGCGGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTAC 6 0.15 No Hit CCCCACTGCTGCCTCCCGTAGGAGTCTGGGCCGTGTCTCAGTCCCAGTGT 6 0.15 No Hit GAGCATTACGTTCGTGCATTACTTCCATACCAAGATTAGCACGGTTGATG 6 0.15 No Hit CTCGCGAATACCATCAATATCTACTGGAGGGGCTGCGATGAAGGCGATAA 6 0.15 No Hit CGCGAATACCATCAATATCTACTGGAGGGGCTGCGATGAAGGCGATAATA 5 0.125 No Hit GTGCAATCCGATTGCCGCAGAAGTAGGAATAATAGCACCAGAGATAATAT 5 0.125 No Hit AGATAATATTGTTTCCATAAAGTAAAGAACCAGAAACAGGCTCGCGAATA 5 0.125 No Hit CTGCGATGAAGGCGATAATAAATACAGAAGTTGCGGTCAATAAGGTAGGG 5 0.125 No Hit GCCCTCATTAGCAGATTCATTTTCAGTAGTTTCCCTGATCAAACTAGAGG 5 0.125 No Hit GTCTCTTCCTCTTGACCAAATTTGTAACCCTCATTAGCAGATTCATTTTC 5 0.125 No Hit GCTGAATATGCAACAGCAATCCAAGGGCGCATACCCAAACGGAAACTAAG 5 0.125 No Hit GTCGGTTTCGGGTACAGGTACCCTTTTGTTGAAGGTCGTTCGAGCTTTTC 5 0.125 No Hit GTTGCGGTCAATAAGGTAGGGATCATCAAAACACCGAACCATCCGATGTA 5 0.125 No Hit CCAAGAAGAAGTGTAAAGAACGAGAGTTGTTGAAACTAGCATATTGGAAG 5 0.125 No Hit ATCAGCCCAAGTATTAATAACGCGACCTTGACTATCAACTACAGATTGGT 5 0.125 No Hit GATGATATCAGCCCAAGTATTAATAACGCGACCTTGACTATCAACTACAG 5 0.125 No Hit CCAGATTCCTACTACAGGCCAAGCAGCCAAGAAGAAGTGTAAAGAACGAG 5 0.125 No Hit GGATCATCAAAACACCGAACCATCCGATGTAAAGACGATTTTCAGTGCTA 5 0.125 No Hit GGGCTGCGATGAAGGCGATAATAAATACAGAAGTTGCGGTCAATAAGGTA 5 0.125 No Hit CATCAGAGAAGCTTCCTTGACCAATAGGGTAAATCAAGAAAACAGCAGTC 5 0.125 No Hit GTGGTTCGCTGGAGTAGGCTGCGAGGCCCAAGCGGCCAAGGAGTGCAGCG 5 0.125 No Hit GTTCAGGGTTCCAAACTCATAGTGGCAACTAAACACGAGGGTTGCGCTCG 5 0.125 No Hit CCCGTCAATTCCTTTGAGTTTCATTCTTGCGAACGTACTCCCCAGGCGGG 5 0.125 No Hit GCCCAATCATTCCGGATAACGCTTGCATCCTCTGTCTTACCGCGGCTGCT 5 0.125 No Hit CCAAGTATTAATAACGCGACCTTGACTATCAACTACAGATTGGTTGAAAT 5 0.125 No Hit GAAAAACCTTATCCATTAATAGCGGGAACTTCAAGAGCAGCTAGATCTAG 5 0.125 No Hit GTTTCCATAAAGTAAAGAACCAGAAACAGGCTCGCGAATACCATCAATAT 5 0.125 No Hit CCACTCACGACCCATATAACAAGCTACACCAAGTAAGAAGTGTAGAACAA 5 0.125 No Hit CATAAGGATGTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAAAGTAC 5 0.125 No Hit GTTCGTGCATTACTTCCATACCAAGATTAGCACGGTTGATGATATCAGCC 5 0.125 No Hit GCCAAGCAGCCAAGAAGAAGTGTAAAGAACGAGAGTTGTTGAAACTAGCA 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.025 0.0 0.0 0.0 0.0 52-53 0.025 0.0 0.0 0.0 0.0 54-55 0.025 0.0 0.0 0.0 0.0 56-57 0.05 0.0 0.0 0.0 0.0 58-59 0.0625 0.0 0.0 0.0 0.0 60-61 0.075 0.0 0.0 0.0 0.0 62-63 0.0875 0.0 0.0 0.0 0.0 64-65 0.1 0.0 0.0 0.0 0.0 66-67 0.15 0.0 0.0 0.0 0.0 68-69 0.21250000000000002 0.0 0.0 0.0 0.0 70-71 0.36250000000000004 0.0 0.0 0.0 0.0 72-73 0.5 0.0 0.0 0.0 0.0 74-75 0.6499999999999999 0.0 0.0 0.0 0.0 76-77 0.8125 0.0 0.0 0.0 0.0 78-79 0.9375 0.0 0.0 0.0 0.0 80-81 1.0125 0.0 0.0 0.0 0.0 82-83 1.1625 0.0 0.0 0.0 0.0 84-85 1.3 0.0 0.0 0.0 0.0 86-87 1.5125 0.0 0.0 0.0 0.0 88-89 1.65 0.0 0.0 0.0 0.0 90-91 1.8250000000000002 0.0 0.0 0.0 0.0 92-93 1.9874999999999998 0.0 0.0 0.0 0.0 94-95 2.2249999999999996 0.0 0.0 0.0 0.0 96-97 2.5125 0.0 0.0 0.0 0.0 98-99 2.85 0.0 0.0 0.0 0.0 100-101 3.2375 0.0 0.0 0.0 0.0 102-103 3.4625 0.0 0.0 0.0 0.0 104-105 3.825 0.0 0.0 0.0 0.0 106-107 4.1125 0.0 0.0 0.0 0.0 108-109 4.5 0.0 0.0 0.0 0.0 110-111 4.824999999999999 0.0 0.0 0.0 0.0 112-113 5.3375 0.0 0.0 0.0 0.0 114-115 5.625 0.0 0.0 0.0 0.0 116-117 5.9375 0.0 0.0 0.0 0.0 118-119 6.225 0.0 0.0 0.0 0.0 120-121 6.7125 0.0 0.0 0.0 0.0 122-123 7.1125 0.0 0.0 0.0 0.0 124-125 7.6125 0.0 0.0 0.0 0.0 126-127 8.0625 0.0 0.0 0.0 0.0 128-129 8.45 0.0 0.0 0.0 0.0 130-131 8.875 0.0 0.0 0.0 0.0 132-133 9.2125 0.0 0.0 0.0 0.0 134-135 9.4375 0.0 0.0 0.0 0.0 136-137 9.8 0.0 0.0 0.0 0.0 138-139 10.3625 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TTTCTTT 10 0.0065874006 146.74684 2 TTCTTTT 10 0.0065874006 146.74684 3 AAAAAAA 35 0.003549008 20.701786 135-139 >>END_MODULE SRR6941562 read2 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR6941562_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 33.7015 35.0 35.0 35.0 33.0 35.0 2 34.03825 35.0 35.0 35.0 33.0 35.0 3 34.28875 35.0 35.0 35.0 33.0 35.0 4 34.2155 35.0 35.0 35.0 33.0 35.0 5 34.344 35.0 35.0 35.0 33.0 35.0 6 38.9365 40.0 40.0 40.0 39.0 40.0 7 38.8675 40.0 40.0 40.0 38.0 40.0 8 39.04125 40.0 40.0 40.0 39.0 40.0 9 39.13975 40.0 40.0 40.0 39.0 40.0 10-14 39.075250000000004 40.0 40.0 40.0 38.8 40.0 15-19 39.16619999999999 40.0 40.0 40.0 39.0 40.0 20-24 39.137100000000004 40.0 40.0 40.0 39.0 40.0 25-29 39.148649999999996 40.0 40.0 40.0 39.0 40.0 30-34 39.0382 40.0 40.0 40.0 38.8 40.0 35-39 38.9128 40.0 39.8 40.0 38.4 40.0 40-44 39.042500000000004 40.0 40.0 40.0 38.8 40.0 45-49 38.967349999999996 40.0 40.0 40.0 38.4 40.0 50-54 38.8045 40.0 39.6 40.0 37.6 40.0 55-59 38.81245 40.0 39.8 40.0 37.4 40.0 60-64 38.7666 40.0 39.4 40.0 37.6 40.0 65-69 38.80225 40.0 39.4 40.0 37.6 40.0 70-74 38.74345 40.0 39.2 40.0 37.2 40.0 75-79 38.678549999999994 40.0 39.4 40.0 37.0 40.0 80-84 38.70890000000001 40.0 39.0 40.0 37.4 40.0 85-89 38.78915000000001 40.0 39.4 40.0 37.8 40.0 90-94 38.72625000000001 40.0 39.0 40.0 37.4 40.0 95-99 38.560849999999995 40.0 39.0 40.0 36.6 40.0 100-104 37.23295 38.6 37.8 39.2 33.6 39.4 105-109 38.55055 40.0 39.0 40.0 36.4 40.0 110-114 38.5986 40.0 39.0 40.0 36.8 40.0 115-119 38.493449999999996 40.0 39.0 40.0 36.4 40.0 120-124 38.35215 40.0 39.0 40.0 36.0 40.0 125-129 38.461400000000005 40.0 39.0 40.0 36.6 40.0 130-134 38.2581 40.0 39.0 40.0 36.0 40.0 135-139 38.141200000000005 40.0 39.0 40.0 35.8 40.0 140-144 37.768350000000005 40.0 39.0 40.0 34.8 40.0 145-149 37.3624 40.0 38.8 40.0 33.4 40.0 150-151 34.41525 38.0 34.5 39.5 24.5 40.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 7.0 3 1.0 4 0.0 5 0.0 6 0.0 7 0.0 8 2.0 9 1.0 10 0.0 11 2.0 12 1.0 13 0.0 14 0.0 15 2.0 16 0.0 17 3.0 18 3.0 19 1.0 20 0.0 21 2.0 22 6.0 23 6.0 24 5.0 25 7.0 26 24.0 27 18.0 28 23.0 29 21.0 30 26.0 31 36.0 32 45.0 33 56.0 34 50.0 35 61.0 36 100.0 37 161.0 38 338.0 39 2992.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 52.46945010183299 19.90835030549898 10.005091649694501 17.617107942973522 2 25.490689481630596 21.967790639154504 32.51132360342224 20.03019627579265 3 21.55 24.25 37.075 17.125 4 25.224999999999998 31.025000000000002 25.374999999999996 18.375 5 26.375 31.225 24.2 18.2 6 21.725 34.2 27.075 17.0 7 18.875 21.05 40.625 19.45 8 20.95 24.625 29.125 25.3 9 22.225 21.975 32.925 22.875 10-14 24.075 25.485000000000003 30.669999999999998 19.77 15-19 23.855 24.525 31.195 20.424999999999997 20-24 24.535 24.93 30.880000000000003 19.655 25-29 24.305 25.405 29.520000000000003 20.77 30-34 24.895 24.4 30.72 19.985 35-39 25.215 24.325 29.95 20.51 40-44 24.245 25.34 30.020000000000003 20.395 45-49 24.0 25.259999999999998 30.294999999999998 20.445 50-54 24.025 25.235000000000003 30.165 20.575 55-59 23.875 26.85 28.53 20.745 60-64 23.825 24.945 30.335 20.895 65-69 24.27 25.009999999999998 29.865000000000002 20.855 70-74 24.86 25.230000000000004 29.475 20.435 75-79 24.395 24.845 29.34 21.42 80-84 24.75 24.125 31.755 19.37 85-89 25.595000000000002 25.27 29.189999999999998 19.945 90-94 24.715 24.915000000000003 28.73 21.64 95-99 24.4 25.180000000000003 29.015 21.404999999999998 100-104 24.37 25.905 29.595 20.13 105-109 25.935000000000002 23.990000000000002 29.904999999999998 20.169999999999998 110-114 25.205 25.4 29.439999999999998 19.955000000000002 115-119 25.53 24.490000000000002 30.135 19.845 120-124 24.845 26.200000000000003 28.185 20.77 125-129 24.915000000000003 25.759999999999998 28.74 20.585 130-134 25.25 25.55 28.939999999999998 20.26 135-139 25.5 25.509999999999998 29.435 19.555 140-144 25.155 25.535000000000004 29.865000000000002 19.445 145-149 24.995 26.040000000000003 29.385 19.580000000000002 150-151 24.840285606914694 25.21608417888012 29.87598647125141 20.067643742953777 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.5 4 0.5 5 0.5 6 0.5 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.5 19 0.5 20 0.0 21 2.0 22 2.0 23 0.0 24 1.5 25 5.5 26 8.5 27 11.0 28 14.0 29 19.5 30 25.5 31 29.0 32 27.5 33 35.5 34 60.0 35 70.0 36 93.5 37 147.0 38 180.0 39 213.0 40 244.5 41 241.5 42 208.5 43 224.5 44 252.5 45 208.5 46 186.0 47 158.5 48 116.5 49 93.0 50 79.0 51 87.0 52 77.0 53 67.5 54 92.5 55 117.0 56 117.0 57 92.0 58 68.0 59 58.5 60 51.0 61 49.0 62 47.5 63 29.5 64 15.5 65 16.0 66 8.0 67 5.0 68 9.0 69 7.5 70 5.0 71 4.0 72 2.0 73 2.0 74 3.0 75 2.5 76 2.0 77 1.0 78 1.0 79 1.0 80 0.0 81 0.0 82 0.0 83 0.5 84 0.5 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 1.7999999999999998 2 0.65 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.21250000000000002 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 64.5 #Duplication Level Percentage of deduplicated Percentage of total 1 82.32558139534883 53.1 2 7.635658914728682 9.85 3 3.6821705426356592 7.124999999999999 4 1.8992248062015504 4.9 5 1.0077519379844961 3.25 6 1.0077519379844961 3.9 7 0.5426356589147286 2.45 8 0.3488372093023256 1.7999999999999998 9 0.23255813953488372 1.35 >10 1.317829457364341 12.275 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GCACAACATCCTTATGCATCCATTTCACATGTTAGGTGTAGCTGGTGTTT 23 0.575 No Hit GTTCGATCCTGGCTCAGGATGAACGCTGGCGGCATGCTTAACACATGCAA 22 0.5499999999999999 No Hit TATCTATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTT 20 0.5 No Hit GTTATTTTGGCCGATTAATCTTCCAATATGCTAGTTTCAACAACTCTCGT 19 0.475 No Hit GTGGGAACTTAGTTTCCGTTTGGGTATGCGCCCTTGGATTGCTGTTGCAT 19 0.475 No Hit GTTTCAACAACTCTCGTTCTTTACACTTCTTCTTGGCTGCTTGGCCTGTA 18 0.44999999999999996 No Hit ATTATCTATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAAT 18 0.44999999999999996 No Hit ATCTATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTG 18 0.44999999999999996 No Hit AGCACAACATCCTTATGCATCCATTTCACATGTTAGGTGTAGCTGGTGTT 17 0.42500000000000004 No Hit GCTAGTTTCAACAACTCTCGTTCTTTACACTTCTTCTTGGCTGCTTGGCC 17 0.42500000000000004 No Hit CTATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAA 17 0.42500000000000004 No Hit GTTGCATATTCAGCTCCTGTTGCAGCTGCGACTGCTGTTTTCTTGATTTA 17 0.42500000000000004 No Hit GCTTGTTATATGGGTCGTGAGTGGGAACTTAGTTTCCGTTTGGGTATGCG 16 0.4 No Hit GTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGATCTT 15 0.375 No Hit GGGAAACTACTGAAAATGAATCTGCTAATGAGGGTTACAAATTTGGTCAA 15 0.375 No Hit AGGGAAACTACTGAAAATGAATCTGCTAATGAGGGTTACAAATTTGGTCA 15 0.375 No Hit GTTAGGTGTAGCTGGTGTTTTCGGCGGTTCCCTATTCAGTGCTATGCATG 15 0.375 No Hit GTCAAGGTCGCGTTATTAATACTTGGGCTGATATCATCAACCGTGCTAAT 14 0.35000000000000003 No Hit GTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGATCTTACCATGAC 14 0.35000000000000003 No Hit GGAAGAGACTTATAATATTGTGGCTGCTCATGGTTATTTTGGCCGATTAA 13 0.325 No Hit GCTGTTTTCTTGATTTACCCTATTGGTCAAGGAAGCTTCTCTGATGGTAT 13 0.325 No Hit ATTTGGTCAAGAGGAAGAGACTTATAATATTGTGGCTGCTCATGGTTATT 12 0.3 No Hit GCTGCATCCGTTGATGAATGGTTATACAATGGTGGTCCTTATGAGCTAAT 12 0.3 No Hit GCTGCGACTGCTGTTTTCTTGATTTACCCTATTGGTCAAGGAAGCTTCTC 11 0.27499999999999997 No Hit GTTTGATCAGGGAAACTACTGAAAATGAATCTGCTAATGAGGGTTACAAA 11 0.27499999999999997 No Hit GTAGCTGGTGTTTTCGGCGGTTCCCTATTCAGTGCTATGCATGGTTCCTT 10 0.25 No Hit GAGACTTATAATATTGTGGCTGCTCATGGTTATTTTGGCCGATTAATCTT 10 0.25 No Hit ATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCTAAATGG 10 0.25 No Hit GGAAGCTTCTCTGATGGTATGCCTTTAGGAATATCTGGTACTTTCAACTT 10 0.25 No Hit GTAACCTCTAGTTTGATCAGGGAAACTACTGAAAATGAATCTGCTAATGA 10 0.25 No Hit GTAGGAATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCT 10 0.25 No Hit TCTATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGA 10 0.25 No Hit GGTACTTTCAACTTTATGATTGTATTCCAGGCAGAGCACAACATCCTTAT 10 0.25 No Hit GTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGATCTTACCATGACTG 10 0.25 No Hit GTTTTGATGATCCCTACCTTATTGACCGCAACTTCTGTATTTATTATCGC 9 0.22499999999999998 No Hit GCATGGTTCCTTGGTAACCTCTAGTTTGATCAGGGAAACTACTGAAAATG 9 0.22499999999999998 No Hit GTAGCTTGTTATATGGGTCGTGAGTGGGAACTTAGTTTCCGTTTGGGTAT 9 0.22499999999999998 No Hit GGCTTGCGGTGGATACCTAGGCACCCAGAGACGAGGAAGGGCGTAGCAAG 9 0.22499999999999998 No Hit CCTTACCAGGGCTTGACATGCCGCGAATCCTCTTGAAAGAGAGGGGTGCC 9 0.22499999999999998 No Hit GTTCAACCTAAATGGATTCAATTTCAACCAATCTGTAGTTGATAGTCAAG 9 0.22499999999999998 No Hit GGGAGTCCTTGCAATTTGAATAAACCAAGATCTTACCATGACTGCAATTT 8 0.2 No Hit ATCGGATTGCACTTTTACCCAATTTGGGAAGCTGCATCCGTTGATGAATG 8 0.2 No Hit GGAAACTACTGAAAATGAATCTGCTAATGAGGGTTACAAATTTGGTCAAG 8 0.2 No Hit GACTTATAATATTGTGGCTGCTCATGGTTATTTTGGCCGATTAATCTTCC 8 0.2 No Hit AGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGATCT 8 0.2 No Hit CAGTGCTATGCATGGTTCCTTGGTAACCTCTAGTTTGATCAGGGAAACTA 8 0.2 No Hit GAGGAAGAGACTTATAATATTGTGGCTGCTCATGGTTATTTTGGCCGATT 8 0.2 No Hit CAGGGAAACTACTGAAAATGAATCTGCTAATGAGGGTTACAAATTTGGTC 8 0.2 No Hit GAATGGTTATACAATGGTGGTCCTTATGAGCTAATTGTTCTACACTTCTT 8 0.2 No Hit GGTCGCTTCTGCAACTGGATAACTAGCACTGAAAATCGTCTTTACATCGG 7 0.17500000000000002 No Hit GCTCATGGTTATTTTGGCCGATTAATCTTCCAATATGCTAGTTTCAACAA 7 0.17500000000000002 No Hit CCGCAACTTCTGTATTTATTATCGCCTTCATCGCAGCCCCTCCAGTAGAT 7 0.17500000000000002 No Hit CCTAAATGGATTCAATTTCAACCAATCTGTAGTTGATAGTCAAGGTCGCG 7 0.17500000000000002 No Hit GGAATATCTGGTACTTTCAACTTTATGATTGTATTCCAGGCAGAGCACAA 7 0.17500000000000002 No Hit TGAAAATGAATCTGCTAATGAGGGTTACAAATTTGGTCAAGAGGAAGAGA 7 0.17500000000000002 No Hit GTTATATGGGTCGTGAGTGGGAACTTAGTTTCCGTTTGGGTATGCGCCCT 7 0.17500000000000002 No Hit GTGCTATGCATGGTTCCTTGGTAACCTCTAGTTTGATCAGGGAAACTACT 7 0.17500000000000002 No Hit GTTGATAGTCAAGGTCGCGTTATTAATACTTGGGCTGATATCATCAACCG 7 0.17500000000000002 No Hit TGTAGCTGGTGTTTTCGGCGGTTCCCTATTCAGTGCTATGCATGGTTCCT 7 0.17500000000000002 No Hit GTTGATGAATGGTTATACAATGGTGGTCCTTATGAGCTAATTGTTCTACA 7 0.17500000000000002 No Hit GGTTCTTTACTTTATGGAAACAATATTATCTCTGGTGCTATTATTCCTAC 7 0.17500000000000002 No Hit GAAGAGACTTATAATATTGTGGCTGCTCATGGTTATTTTGGCCGATTAAT 7 0.17500000000000002 No Hit GTTCTACACTTCTTACTTGGTGTAGCTTGTTATATGGGTCGTGAGTGGGA 7 0.17500000000000002 No Hit GAAAGTACAAGCCTGTGGGGTCGCTTCTGCAACTGGATAACTAGCACTGA 6 0.15 No Hit GTTTTCTTGATTTACCCTATTGGTCAAGGAAGCTTCTCTGATGGTATGCC 6 0.15 No Hit GTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCTAAATGGATTCA 6 0.15 No Hit GCGCCCTTGGATTGCTGTTGCATATTCAGCTCCTGTTGCAGCTGCGACTG 6 0.15 No Hit GCTAATTGTTCTACACTTCTTACTTGGTGTAGCTTGTTATATGGGTCGTG 6 0.15 No Hit CTGGTACTTTCAACTTTATGATTGTATTCCAGGCAGAGCACAACATCCTT 6 0.15 No Hit GTCGTGAGTGGGAACTTAGTTTCCGTTTGGGTATGCGCCCTTGGATTGCT 6 0.15 No Hit ATTTCAACCAATCTGTAGTTGATAGTCAAGGTCGCGTTATTAATACTTGG 6 0.15 No Hit CCAAGATCTTACCATGACTGCAATTTTAGAGAGACGCGAAAGTACAAGCC 6 0.15 No Hit GTACTATGGCGTTCAACCTAAATGGATTCAATTTCAACCAATCTGTAGTT 6 0.15 No Hit CCTGGCTCAGGATGAACGCTGGCGGCATGCTTAACACATGCAAGTCGAAC 6 0.15 No Hit GGGTTACAAATTTGGTCAAGAGGAAGAGACTTATAATATTGTGGCTGCTC 6 0.15 No Hit CCTGTAGTAGGAATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTT 6 0.15 No Hit GGCCTGTAGTAGGAATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCG 6 0.15 No Hit TTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAG 6 0.15 No Hit AATCGGATTGCACTTTTACCCAATTTGGGAAGCTGCATCCGTTGATGAAT 6 0.15 No Hit GTTATACAATGGTGGTCCTTATGAGCTAATTGTTCTACACTTCTTACTTG 6 0.15 No Hit CTAGCACTGAAAATCGTCTTTACATCGGATGGTTCGGTGTTTTGATGATC 6 0.15 No Hit GGAATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCTAAA 6 0.15 No Hit GGTGTAGCTTGTTATATGGGTCGTGAGTGGGAACTTAGTTTCCGTTTGGG 6 0.15 No Hit GCCTTTAGGAATATCTGGTACTTTCAACTTTATGATTGTATTCCAGGCAG 6 0.15 No Hit ATTCAGTGCTATGCATGGTTCCTTGGTAACCTCTAGTTTGATCAGGGAAA 6 0.15 No Hit GTTGGGTTAAGTCTCGCAACGAGCGCAACCCTCGTGTTTAGTTGCCACTA 6 0.15 No Hit CACATGTTAGGTGTAGCTGGTGTTTTCGGCGGTTCCCTATTCAGTGCTAT 6 0.15 No Hit GTTCTTTACTTTATGGAAACAATATTATCTCTGGTGCTATTATTCCTACT 6 0.15 No Hit GCTAACTCCAAAAACCCGTCCTCAGTTCGGATTGCAGGCTGCAACTCGCC 6 0.15 No Hit GTATGCGCCCTTGGATTGCTGTTGCATATTCAGCTCCTGTTGCAGCTGCG 5 0.125 No Hit CCTACTTCTGCGGCAATCGGATTGCACTTTTACCCAATTTGGGAAGCTGC 5 0.125 No Hit GCTTGGGAGTCCTTGCAATTTGAATAAACCAAGATCTTACCATGACTGCA 5 0.125 No Hit GATGGTATTCGCGAGCCTGTTTCTGGTTCTTTACTTTATGGAAACAATAT 5 0.125 No Hit CATCCGTTGATGAATGGTTATACAATGGTGGTCCTTATGAGCTAATTGTT 5 0.125 No Hit AGTACTATGGCGTTCAACCTAAATGGATTCAATTTCAACCAATCTGTAGT 5 0.125 No Hit GTTCCTTGGTAACCTCTAGTTTGATCAGGGAAACTACTGAAAATGAATCT 5 0.125 No Hit GCAATTTTAGAGAGACGCGAAAGTACAAGCCTGTGGGGTCGCTTCTGCAA 5 0.125 No Hit TATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAAT 5 0.125 No Hit GCGACTGCTGTTTTCTTGATTTACCCTATTGGTCAAGGAAGCTTCTCTGA 5 0.125 No Hit GAGGGTTACAAATTTGGTCAAGAGGAAGAGACTTATAATATTGTGGCTGC 5 0.125 No Hit GCGAGAGCTTGTAACCCGAGTGGGGGCTTTAAGGGTGGTGTGGACGTTGC 5 0.125 No Hit GCTAATGAGGGTTACAAATTTGGTCAAGAGGAAGAGACTTATAATATTGT 5 0.125 No Hit ATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAA 5 0.125 No Hit AAACAATATTATCTCTGGTGCTATTATTCCTACTTCTGCGGCAATCGGAT 5 0.125 No Hit ATTTCACATGTTAGGTGTAGCTGGTGTTTTCGGCGGTTCCCTATTCAGTG 5 0.125 No Hit GGGAACTTAGTTTCCGTTTGGGTATGCGCCCTTGGATTGCTGTTGCATAT 5 0.125 No Hit CAATGGTGGTCCTTATGAGCTAATTGTTCTACACTTCTTACTTGGTGTAG 5 0.125 No Hit GTTTCTGGTTCTTTACTTTATGGAAACAATATTATCTCTGGTGCTATTAT 5 0.125 No Hit ATTGTATTCCAGGCAGAGCACAACATCCTTATGCATCCATTTCACATGTT 5 0.125 No Hit GTCAAGAGGAAGAGACTTATAATATTGTGGCTGCTCATGGTTATTTTGGC 5 0.125 No Hit GGATAACTAGCACTGAAAATCGTCTTTACATCGGATGGTTCGGTGTTTTG 5 0.125 No Hit ATTGTGGCTGCTCATGGTTATTTTGGCCGATTAATCTTCCAATATGCTAG 5 0.125 No Hit GAAGCTTCTCTGATGGTATGCCTTTAGGAATATCTGGTACTTTCAACTTT 5 0.125 No Hit GCCGATTAATCTTCCAATATGCTAGTTTCAACAACTCTCGTTCTTTACAC 5 0.125 No Hit GAATAAACCAAGATCTTACCATGACTGCAATTTTAGAGAGACGCGAAAGT 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.025 0.0 0.0 0.0 0.0 52-53 0.025 0.0 0.0 0.0 0.0 54-55 0.025 0.0 0.0 0.0 0.0 56-57 0.05 0.0 0.0 0.0 0.0 58-59 0.0625 0.0 0.0 0.0 0.0 60-61 0.075 0.0 0.0 0.0 0.0 62-63 0.1125 0.0 0.0 0.0 0.0 64-65 0.125 0.0 0.0 0.0 0.0 66-67 0.175 0.0 0.0 0.0 0.0 68-69 0.2375 0.0 0.0 0.0 0.0 70-71 0.38749999999999996 0.0 0.0 0.0 0.0 72-73 0.525 0.0 0.0 0.0 0.0 74-75 0.675 0.0 0.0 0.0 0.0 76-77 0.8375 0.0 0.0 0.0 0.0 78-79 0.95 0.0 0.0 0.0 0.0 80-81 1.0125 0.0 0.0 0.0 0.0 82-83 1.1875 0.0 0.0 0.0 0.0 84-85 1.325 0.0 0.0 0.0 0.0 86-87 1.5625 0.0 0.0 0.0 0.0 88-89 1.7125 0.0 0.0 0.0 0.0 90-91 1.9 0.0 0.0 0.0 0.0 92-93 2.0625 0.0 0.0 0.0 0.0 94-95 2.3 0.0 0.0 0.0 0.0 96-97 2.5625 0.0 0.0 0.0 0.0 98-99 2.875 0.0 0.0 0.0 0.0 100-101 3.3 0.0 0.0 0.0 0.0 102-103 3.5375 0.0 0.0 0.0 0.0 104-105 3.9000000000000004 0.0 0.0 0.0 0.0 106-107 4.2375 0.0 0.0 0.0 0.0 108-109 4.7 0.0 0.0 0.0 0.0 110-111 5.012499999999999 0.0 0.0 0.0 0.0 112-113 5.4875 0.0 0.0 0.0 0.0 114-115 5.775 0.0 0.0 0.0 0.0 116-117 6.0875 0.0 0.0 0.0 0.0 118-119 6.375 0.0 0.0 0.0 0.0 120-121 6.8625 0.0 0.0 0.0 0.0 122-123 7.2625 0.0 0.0 0.0 0.0 124-125 7.7375 0.0 0.0 0.0 0.0 126-127 8.149999999999999 0.0 0.0 0.0 0.0 128-129 8.525 0.0 0.0 0.0 0.0 130-131 8.95 0.0 0.0 0.0 0.0 132-133 9.2875 0.0 0.0 0.0 0.0 134-135 9.5125 0.0 0.0 0.0 0.0 136-137 9.85 0.0 0.0 0.0 0.0 138-139 10.4375 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAATTCA 10 0.0068343505 144.975 3 ATCTATA 15 1.1419188E-4 144.97499 1 TCTATAT 15 1.1419188E-4 144.97499 2 CTATATT 20 3.5901665E-4 108.73125 3 TATATTA 20 3.5901665E-4 108.73125 4 TTATTTC 20 3.5901665E-4 108.73125 8 ATTATTT 20 3.5901665E-4 108.73125 7 TATTTCT 20 3.5901665E-4 108.73125 9 TATTATT 30 0.0017985795 72.487495 6 ATATTAT 30 0.0017985795 72.487495 5 >>END_MODULE Read 1669959 spots for SRR6941562.sra Written 1669959 spots for SRR6941562.sra Read 1669959 spots for SRR6941562.sra Written 1669959 spots for SRR6941562.sra Read 1669959 spots for SRR6941562.sra Written 1669959 spots for SRR6941562.sra Read 1669959 spots for SRR6941562.sra Written 1669959 spots for SRR6941562.sra Read 1669959 spots for SRR6941562.sra Written 1669959 spots for SRR6941562.sra Read 1669959 spots for SRR6941562.sra Written 1669959 spots for SRR6941562.sra Read 1669959 spots for SRR6941562.sra Written 1669959 spots for SRR6941562.sra Read 1669959 spots for SRR6941562.sra Written 1669959 spots for SRR6941562.sra Read 1669960 spots for SRR6941562.sra Written 1669960 spots for SRR6941562.sra Read 1669959 spots for SRR6941562.sra Written 1669959 spots for SRR6941562.sra Read 1669959 spots for SRR6941562.sra Written 1669959 spots for SRR6941562.sra Read 1669959 spots for SRR6941562.sra Written 1669959 spots for SRR6941562.sra Read 1669959 spots for SRR6941562.sra Written 1669959 spots for SRR6941562.sra Read 1669959 spots for SRR6941562.sra Written 1669959 spots for SRR6941562.sra Read 1669959 spots for SRR6941562.sra Written 1669959 spots for SRR6941562.sra Read 1669959 spots for SRR6941562.sra Written 1669959 spots for SRR6941562.sra Read 1669959 spots for SRR6941562.sra Written 1669959 spots for SRR6941562.sra Read 1669959 spots for SRR6941562.sra Written 1669959 spots for SRR6941562.sra Read 1669959 spots for SRR6941562.sra Written 1669959 spots for SRR6941562.sra Read 1669959 spots for SRR6941562.sra Written 1669959 spots for SRR6941562.sra SRR ids: ['SRR6941562.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd__pyed6n3 SRR6941562.sra spots: 33399181 blocks: [[1, 1669959], [1669960, 3339918], [3339919, 5009877], [5009878, 6679836], [6679837, 8349795], [8349796, 10019754], [10019755, 11689713], [11689714, 13359672], [13359673, 15029631], [15029632, 16699590], [16699591, 18369549], [18369550, 20039508], [20039509, 21709467], [21709468, 23379426], [23379427, 25049385], [25049386, 26719344], [26719345, 28389303], [28389304, 30059262], [30059263, 31729221], [31729222, 33399181]] SRR6941562 file size 11296186 SRR6941562 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR6941562 SRR6941562_1.fastq SRR6941562_2.fastq Input file: SRR6941562_1.fastq Paired file: SRR6941562_2.fastq trimmed: SRR6941562-trimmed-pair1.fastq, SRR6941562-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Fri Dec 6 11:25:54 2024 >> started Fri Dec 6 11:26:32 2024 >> done (37.669s) 33399181 read pairs processed; of these: 18210 ( 0.05%) short read pairs filtered out after trimming by size control 29740 ( 0.09%) empty read pairs filtered out after trimming by size control 33351231 (99.86%) read pairs available; of these: 7742573 (23.22%) trimmed read pairs available after processing 25608658 (76.78%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 3 0.00% 19 1 0.00% 20 2 0.00% 21 8 0.00% 22 8 0.00% 23 5 0.00% 24 15 0.00% 25 9 0.00% 26 9 0.00% 27 16 0.00% 28 16 0.00% 29 30 0.00% 30 36 0.00% 31 32 0.00% 32 36 0.00% 33 47 0.00% 34 49 0.00% 35 46 0.00% 36 83 0.00% 37 102 0.00% 38 115 0.00% 39 147 0.00% 40 200 0.00% 41 215 0.00% 42 245 0.00% 43 270 0.00% 44 304 0.00% 45 292 0.00% 46 436 0.00% 47 473 0.00% 48 547 0.00% 49 683 0.00% 50 739 0.00% 51 877 0.00% 52 1078 0.00% 53 1101 0.00% 54 1348 0.00% 55 1480 0.00% 56 1561 0.00% 57 2005 0.01% 58 2091 0.01% 59 2411 0.01% 60 2757 0.01% 61 3324 0.01% 62 4134 0.01% 63 4510 0.01% 64 5125 0.02% 65 5641 0.02% 66 6047 0.02% 67 6287 0.02% 68 7369 0.02% 69 8851 0.03% 70 9744 0.03% 71 11193 0.03% 72 13290 0.04% 73 14263 0.04% 74 13954 0.04% 75 15875 0.05% 76 16003 0.05% 77 18707 0.06% 78 18146 0.05% 79 19608 0.06% 80 22190 0.07% 81 23867 0.07% 82 26748 0.08% 83 26825 0.08% 84 28485 0.09% 85 33775 0.10% 86 34257 0.10% 87 35911 0.11% 88 39864 0.12% 89 39179 0.12% 90 45453 0.14% 91 43964 0.13% 92 50487 0.15% 93 51656 0.15% 94 53717 0.16% 95 56642 0.17% 96 54239 0.16% 97 53287 0.16% 98 51714 0.16% 99 53893 0.16% 100 55654 0.17% 101 59537 0.18% 102 64807 0.19% 103 63663 0.19% 104 65876 0.20% 105 68225 0.20% 106 64821 0.19% 107 66875 0.20% 108 68678 0.21% 109 72716 0.22% 110 73634 0.22% 111 80754 0.24% 112 82311 0.25% 113 79486 0.24% 114 86640 0.26% 115 78500 0.24% 116 79158 0.24% 117 76106 0.23% 118 76807 0.23% 119 74547 0.22% 120 78228 0.23% 121 78149 0.23% 122 94354 0.28% 123 96230 0.29% 124 97993 0.29% 125 98049 0.29% 126 90197 0.27% 127 89700 0.27% 128 89026 0.27% 129 97154 0.29% 130 94112 0.28% 131 104457 0.31% 132 99795 0.30% 133 90683 0.27% 134 103039 0.31% 135 98439 0.30% 136 103123 0.31% 137 98595 0.30% 138 110123 0.33% 139 107255 0.32% 140 101235 0.30% 141 122220 0.37% 142 108139 0.32% 143 116008 0.35% 144 113716 0.34% 145 130261 0.39% 146 139834 0.42% 147 147056 0.44% 148 181854 0.55% 149 260340 0.78% 150 1914262 5.74% 151 25608658 76.78% 33351231 reads passed initial QC criterion=sequence-density sequence-density=0.30 sequence-density-rank=1 fanout-score=4.97 fanout-score-rank=20 prefix-density=0.80 prefix-fanout=1.9 sequence=ATTTAGCCTTGGACGGAGTCTACCGCCCGATTTGGGCTGCATTCCCAAACAACCCGACTCGTTGACGGCGCCTCGTGGGGCGACAGGGTCCGGGCCGGACGGGGCTCTCACCCTCCCAGGCGCCCCTTTCCAGGGGACTTGGGCCCGGTCCGTCGCTGAGGACGCCTCTCCAGACTACAATTCGGACGGCACGGCCGCCCGATTCTCAAGCTGGGCTGCTCCCGGTTCGCTCGCCGTTACTAGGGGAATCCTTGTAAGTTTCTTCTCCTCCGCTTATTTATATGCTTAAACTCAGCGGGTAGTCCCGCCTGACCTG criterion=fanout-score sequence-density=0.01 sequence-density-rank=41 fanout-score=49.11 fanout-score-rank=1 prefix-density=0.09 prefix-fanout=3.9 sequence=CTTTTTATTTTGTTTCTTTTTATTTAGACCTTCTTCATATTTAGTTTTATCTATTAATCGATAGTATCTACCGGCGCGAACTCGAATTTGATCGCCTTCCATACTTCACAAGCTGCGGCTAGTTCAGGACTCCATTTGCAAGCTGCTCGGATAATTTCATTACCTTCACGAGCAAGATCGCGCCCTTCGTTACGAGCTTGTACACAGGCTTCTAAAGCCACTCGATTAGCTGCTGCACCAGGTGCATTTCCCCAAGGATGTCCTAAAGTTCCTCCACCAAATTGTAATACAGAATCATCCCCAAAGATTTCGGTCAGAGCTGGCATATGCCAAACATGAATACCACCTGAAGCTACTGGTATAACACCTGGCATGGATACCCAGTCCTGAGTGAAAAAGATACCGCGAGCACGATCTTTTTCAATAAAATCGTCGCGCAATAAATCAACAAAACCTAAAGTGATTTCGCGTTCCCCTTCTAACTTACCTACTACTGTACCGGCGTGGATAT criterion=sequence-density sequence-density=0.41 sequence-density-rank=1 fanout-score=1.94 fanout-score-rank=30 prefix-density=0.41 prefix-fanout=1.9 sequence=TTCACAGAGCAGCGACAACTGCCCGCTT criterion=fanout-score sequence-density=0.01 sequence-density-rank=41 fanout-score=26.42 fanout-score-rank=1 prefix-density=0.03 prefix-fanout=5.7 sequence=TTTTTTTTTATGAGATTTTTGCTAAAGTTTCATTTACGCCTAATTCACATCGAGTAGACCTTGTTATTGTGAGAATTCTTAATTCAAGAGTTGTAAGGAGGGACTTATGTCACCACAAACAGAAACTAAAGCAAGTGTTGGATTTAAAGCTGGTGTTAAAGATTATAGATTGACTTACTACACCCCGGAGTATGAAACCAAGGATACTGATATCTTGGCAGCATTCCGAGTATCTCCTCAACCTGGGGTTCCGCCCGAAGAAGCAGGGGCTGCAGTAGCTGCCGAATCTTCTACTGGTACATGGACAACTGTTTGGACTGATGGACTTACTAGTCTTGATCGTTACAAAGGACGATGCTATCACATCGAGCCTGTTCCTGGGGAAGACAGTCAATGGATCTGTTATGTAGCTTATCCATTAGATCTATTTGAAGAGGGTTCCGTTACTAACATGTTTACTTCCATTGTAGGTAACGTATTTGGTTTCAAAGCCCTACGTGCTCTACGTCTG SRR6941562 testing PE reads STAR mapping to Ensembl genome Started job on | Dec 06 11:27:17 Started mapping on | Dec 06 11:27:17 Finished on | Dec 06 11:29:54 Mapping speed, Million of reads per hour | 764.74 Number of input reads | 33351231 Average input read length | 291 UNIQUE READS: Uniquely mapped reads number | 19097044 Uniquely mapped reads % | 57.26% Average mapped length | 293.30 Number of splices: Total | 3496534 Number of splices: Annotated (sjdb) | 3037339 Number of splices: GT/AG | 3194926 Number of splices: GC/AG | 43144 Number of splices: AT/AC | 15613 Number of splices: Non-canonical | 242851 Mismatch rate per base, % | 0.24% Deletion rate per base | 0.02% Deletion average length | 1.97 Insertion rate per base | 0.02% Insertion average length | 2.63 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 10918942 % of reads mapped to multiple loci | 32.74% Number of reads mapped to too many loci | 212468 % of reads mapped to too many loci | 0.64% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 6.78% % of reads unmapped: other | 2.59% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 3353795 3353795 3353795 N_multimapping 10918942 10918942 10918942 N_noFeature 9323353 18511142 9612804 N_ambiguous 567522 5568 278312 UnstrandedReadsAssigned:9206169 PositiveStrandReadsAssigned:580334 NegativeStrandReadsAssigned:9205928 Dataset is classified negative stranded MeadianReadLen=151 20thPercentileLength=151 echo kmer=147 SRR6941562 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,972 [index] number of k-mers: 66,720,672 [index] number of equivalence classes: 111,837 [quant] running in paired-end mode [quant] will process pair 1: SRR6941562-trimmed-pair1.fastq SRR6941562-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 33,351,231 reads, 16,266,613 reads pseudoaligned [quant] estimated average fragment length: 219.983 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,073 rounds 52973 SRR6941562.ke.tsv 35125 SRR6941562.se.tsv 88098 total ==> SRR6941562.ke.tsv <== target_id length eff_length est_counts tpm PNS24245 936 717.338 0 0 PNS24247 1044 825.017 16.123 1.00336 PNS24249 1928 1709.02 22.1828 0.666416 PNS24246 1044 825.017 16.123 1.00336 PNS24248 1044 825.017 16.123 1.00336 PNS24244 1471 1252.02 79.4483 3.258 PNS24243 293 108.76 0 0 KQK14069 1603 1384.02 3128.7 116.065 KQK14071 474 261.964 77.7776 15.2437 ==> SRR6941562.se.tsv <== BRADI_1g14170v3 4073 BRADI_1g53295v3 55 BRADI_1g59795v3 84 BRADI_1g07683v3 0 BRADI_1g00485v3 0 BRADI_1g20270v3 92 BRADI_1g74790v3 18 BRADI_1g09890v3 0 BRADI_1g77505v3 73 BRADI_1g48960v3 0 SRR6941562 completed mapping pipeline successfully