Starting /dee2/code/volunteer_pipeline.sh SRR6941563 current disk space = 1551301267456 free memory = 1601888244 SRR6941563 SRAfilesize 0014e39b584401e7a47034c9571aed64 SRR6941563.sra SRR6941563.sra file validated SRR6941563 is paired end SRR6941563 is conventional basespace SRR6941563 read1 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR6941563_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 34.68225 35.0 35.0 35.0 35.0 35.0 2 34.549 35.0 35.0 35.0 35.0 35.0 3 34.687 35.0 35.0 35.0 35.0 35.0 4 34.72825 35.0 35.0 35.0 35.0 35.0 5 34.58825 35.0 35.0 35.0 35.0 35.0 6 39.48925 40.0 40.0 40.0 39.0 40.0 7 39.5185 40.0 40.0 40.0 39.0 40.0 8 39.53925 40.0 40.0 40.0 39.0 40.0 9 39.53625 40.0 40.0 40.0 39.0 40.0 10-14 39.480900000000005 40.0 40.0 40.0 39.0 40.0 15-19 39.43725 40.0 40.0 40.0 39.0 40.0 20-24 39.46745 40.0 40.0 40.0 39.0 40.0 25-29 39.424 40.0 40.0 40.0 39.0 40.0 30-34 39.29165 40.0 40.0 40.0 39.0 40.0 35-39 39.4284 40.0 40.0 40.0 39.0 40.0 40-44 39.403999999999996 40.0 40.0 40.0 39.0 40.0 45-49 39.3803 40.0 40.0 40.0 39.0 40.0 50-54 39.3063 40.0 40.0 40.0 39.0 40.0 55-59 39.27705 40.0 40.0 40.0 39.0 40.0 60-64 39.335449999999994 40.0 40.0 40.0 39.0 40.0 65-69 39.2961 40.0 40.0 40.0 39.0 40.0 70-74 39.18375 40.0 40.0 40.0 38.8 40.0 75-79 39.10275 40.0 40.0 40.0 38.2 40.0 80-84 39.14725 40.0 40.0 40.0 38.6 40.0 85-89 39.13335 40.0 40.0 40.0 38.6 40.0 90-94 39.1005 40.0 40.0 40.0 38.6 40.0 95-99 39.06225 40.0 40.0 40.0 38.2 40.0 100-104 38.162099999999995 39.2 38.6 39.4 36.8 39.8 105-109 39.057 40.0 39.6 40.0 38.2 40.0 110-114 39.11 40.0 40.0 40.0 38.6 40.0 115-119 39.1852 40.0 40.0 40.0 39.0 40.0 120-124 38.908849999999994 40.0 39.8 40.0 37.8 40.0 125-129 38.846450000000004 40.0 39.4 40.0 37.4 40.0 130-134 38.8703 40.0 39.0 40.0 37.6 40.0 135-139 38.76445 40.0 39.0 40.0 37.0 40.0 140-144 38.5283 40.0 39.0 40.0 36.0 40.0 145-149 38.710699999999996 40.0 39.0 40.0 37.0 40.0 150-151 36.88475 39.5 37.0 40.0 32.0 40.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 16 1.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 4.0 24 2.0 25 5.0 26 6.0 27 5.0 28 13.0 29 13.0 30 23.0 31 33.0 32 28.0 33 34.0 34 54.0 35 63.0 36 100.0 37 133.0 38 264.0 39 3219.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 47.93284891004761 10.774242044600351 6.965672763718366 34.327236281633674 2 23.375 13.850000000000001 30.475 32.300000000000004 3 23.075000000000003 17.775 25.7 33.45 4 25.775 26.474999999999998 20.95 26.8 5 24.07917815083939 33.22475570032573 22.400400902029567 20.29566524680531 6 19.875 37.275000000000006 21.675 21.175 7 14.35 30.85 39.074999999999996 15.725 8 16.975 27.525 31.0 24.5 9 16.825000000000003 24.175 36.05 22.95 10-14 19.405 33.665 25.259999999999998 21.67 15-19 20.19 30.85 25.64 23.32 20-24 18.829707426856714 30.257564391097773 27.161790447611907 23.75093773443361 25-29 22.103315497324598 31.18467770165525 25.993899084862733 20.718107716157423 30-34 22.411120556027804 32.34661733086654 24.056202810140505 21.18605930296515 35-39 21.565 30.78 26.66 20.995 40-44 20.244999999999997 30.29 26.245 23.22 45-49 19.985 29.995 28.384999999999998 21.634999999999998 50-54 21.19 31.185000000000002 25.25 22.375 55-59 20.76 30.18 25.765 23.294999999999998 60-64 18.34 30.505 27.400000000000002 23.755000000000003 65-69 20.685000000000002 31.165 25.759999999999998 22.39 70-74 20.54 31.05 23.71 24.7 75-79 20.51 30.04 26.55 22.900000000000002 80-84 22.05 30.04 25.365 22.545 85-89 21.445 29.799999999999997 26.36 22.395 90-94 18.72 30.675 26.845000000000002 23.76 95-99 20.419999999999998 31.995 23.45 24.135 100-104 20.355 32.14 25.009999999999998 22.495 105-109 20.365 31.069999999999997 25.615 22.95 110-114 21.195 29.73 25.34 23.735 115-119 19.97 31.485000000000003 24.6 23.945 120-124 19.91695017010206 30.798479087452474 23.9393636181709 25.345207124274566 125-129 19.51487871967992 31.59789947486872 23.705926481620406 25.18129532383096 130-134 21.34 31.869999999999997 23.535 23.255 135-139 22.37 31.014999999999997 23.990000000000002 22.625 140-144 22.115000000000002 30.520000000000003 25.515 21.85 145-149 20.055 31.205 25.03 23.71 150-151 21.62094450707754 31.617186521357887 22.961292747087562 23.800576224477012 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.5 3 1.0 4 1.0 5 0.5 6 0.5 7 0.5 8 0.5 9 0.5 10 0.0 11 0.0 12 0.5 13 0.5 14 0.0 15 0.0 16 0.5 17 0.5 18 0.0 19 0.5 20 1.5 21 3.5 22 2.5 23 1.0 24 3.0 25 3.0 26 4.0 27 8.0 28 16.5 29 21.5 30 19.5 31 18.0 32 24.0 33 29.0 34 34.5 35 42.5 36 95.5 37 209.0 38 273.5 39 264.5 40 277.0 41 325.5 42 265.0 43 216.5 44 217.0 45 207.0 46 194.0 47 150.5 48 131.5 49 91.0 50 68.0 51 68.0 52 61.0 53 55.0 54 61.5 55 85.0 56 91.0 57 63.5 58 59.0 59 66.5 60 44.0 61 26.0 62 19.5 63 15.0 64 16.5 65 13.0 66 7.5 67 6.5 68 5.0 69 4.0 70 2.5 71 1.5 72 1.5 73 1.5 74 0.5 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.22499999999999998 2 0.0 3 0.0 4 0.0 5 0.22499999999999998 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.025 25-29 0.015 30-34 0.005 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.06 125-129 0.025 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.21250000000000002 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.0 #Duplication Level Percentage of deduplicated Percentage of total 1 83.72950819672131 51.075 2 7.254098360655738 8.85 3 2.8278688524590163 5.175 4 1.8442622950819672 4.5 5 0.6557377049180327 2.0 6 0.7377049180327869 2.7 7 0.5327868852459016 2.275 8 0.5737704918032787 2.8000000000000003 9 0.28688524590163933 1.575 >10 1.5163934426229508 17.474999999999998 >50 0.040983606557377046 1.575 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GCGGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTACG 63 1.575 No Hit GGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTACGTT 45 1.125 No Hit GCTTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCA 39 0.975 No Hit GTGCTCTGCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTCCTA 35 0.8750000000000001 No Hit CTTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCAT 35 0.8750000000000001 No Hit GGCCAAAATAACCATGAGCAGCCACAATATTATAAGTCTCTTCCTCTTGA 31 0.775 No Hit GTTAGCGGAAAAACCTTATCCATTAATAGCGGGAACTTCAAGAGCAGCTA 27 0.675 No Hit CTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCATTAAT 23 0.575 No Hit GGATGCATAAGGATGTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAA 23 0.575 No Hit GCCAAAATAACCATGAGCAGCCACAATATTATAAGTCTCTTCCTCTTGAC 23 0.575 No Hit GTTGAAACTAGCATATTGGAAGATTAATCGGCCAAAATAACCATGAGCAG 22 0.5499999999999999 No Hit CCCAAGTATTAATAACGCGACCTTGACTATCAACTACAGATTGGTTGAAA 21 0.525 No Hit GTGAGCATTACGTTCGTGCATTACTTCCATACCAAGATTAGCACGGTTGA 21 0.525 No Hit GGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTACGTTC 21 0.525 No Hit GCGGAAAAACCTTATCCATTAATAGCGGGAACTTCAAGAGCAGCTAGATC 21 0.525 No Hit CGGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTACGT 20 0.5 No Hit GCGACCTTGACTATCAACTACAGATTGGTTGAAATTGAATCCATTTAGGT 20 0.5 No Hit GACCCATATAACAAGCTACACCAAGTAAGAAGTGTAGAACAATTAGCTCA 18 0.44999999999999996 No Hit GCTCTGCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTCCTAAA 16 0.4 No Hit CTCATAAGGACCACCATTGTATAACCATTCATCAACGGATGCAGCTTCCC 15 0.375 No Hit GCACGGTTGATGATATCAGCCCAAGTATTAATAACGCGACCTTGACTATC 14 0.35000000000000003 No Hit GGCGATAATAAATACAGAAGTTGCGGTCAATAAGGTAGGGATCATCAAAA 14 0.35000000000000003 No Hit GCATAAGGATGTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAAAGTA 14 0.35000000000000003 No Hit GCGGTCAATAAGGTAGGGATCATCAAAACACCGAACCATCCGATGTAAAG 13 0.325 No Hit GTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTC 13 0.325 No Hit GCCGAAAACACCAGCTACACCTAACATGTGAAATGGATGCATAAGGATGT 13 0.325 No Hit TTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCATT 13 0.325 No Hit GTTGATGATATCAGCCCAAGTATTAATAACGCGACCTTGACTATCAACTA 13 0.325 No Hit GTTGAAATTGAATCCATTTAGGTTGAACGCCATAGTACTAATACCTAAAG 13 0.325 No Hit CTCTGCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTCCTAAAG 13 0.325 No Hit GATGCATAAGGATGTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAAA 12 0.3 No Hit CTCGCGAATACCATCAATATCTACTGGAGGGGCTGCGATGAAGGCGATAA 12 0.3 No Hit GCCAAGAAGAAGTGTAAAGAACGAGAGTTGTTGAAACTAGCATATTGGAA 12 0.3 No Hit CGCAGAAGTAGGAATAATAGCACCAGAGATAATATTGTTTCCATAAAGTA 11 0.27499999999999997 No Hit TTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCATTA 11 0.27499999999999997 No Hit CTCTTGACCAAATTTGTAACCCTCATTAGCAGATTCATTTTCAGTAGTTT 11 0.27499999999999997 No Hit CCCCACTGCTGCCTCCCGTAGGAGTCTGGGCCGTGTCTCAGTCCCAGTGT 11 0.27499999999999997 No Hit GGAGGGGCTGCGATGAAGGCGATAATAAATACAGAAGTTGCGGTCAATAA 10 0.25 No Hit CTGCGATGAAGGCGATAATAAATACAGAAGTTGCGGTCAATAAGGTAGGG 9 0.22499999999999998 No Hit CTTGACTATCAACTACAGATTGGTTGAAATTGAATCCATTTAGGTTGAAC 9 0.22499999999999998 No Hit GGGAAGTTGTGAGCATTACGTTCGTGCATTACTTCCATACCAAGATTAGC 9 0.22499999999999998 No Hit CCAGATTCCTACTACAGGCCAAGCAGCCAAGAAGAAGTGTAAAGAACGAG 9 0.22499999999999998 No Hit CCACAATATTATAAGTCTCTTCCTCTTGACCAAATTTGTAACCCTCATTA 9 0.22499999999999998 No Hit AGCGGAAAAACCTTATCCATTAATAGCGGGAACTTCAAGAGCAGCTAGAT 9 0.22499999999999998 No Hit GCCCAAGTATTAATAACGCGACCTTGACTATCAACTACAGATTGGTTGAA 9 0.22499999999999998 No Hit CCGCAGAAGTAGGAATAATAGCACCAGAGATAATATTGTTTCCATAAAGT 8 0.2 No Hit GGTTGATGATATCAGCCCAAGTATTAATAACGCGACCTTGACTATCAACT 8 0.2 No Hit GTCGCAGCTGCAACAGGAGCTGAATATGCAACAGCAATCCAAGGGCGCAT 8 0.2 No Hit CATATAACAAGCTACACCAAGTAAGAAGTGTAGAACAATTAGCTCATAAG 8 0.2 No Hit GGTCAATAAGGTAGGGATCATCAAAACACCGAACCATCCGATGTAAAGAC 8 0.2 No Hit CGGAAAAACCTTATCCATTAATAGCGGGAACTTCAAGAGCAGCTAGATCT 8 0.2 No Hit GAGTTGTTGAAACTAGCATATTGGAAGATTAATCGGCCAAAATAACCATG 8 0.2 No Hit TGCATAAGGATGTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAAAGT 8 0.2 No Hit GGCCAAGCAGCCAAGAAGAAGTGTAAAGAACGAGAGTTGTTGAAACTAGC 8 0.2 No Hit GCTAGATCTAGAGGGAAGTTGTGAGCATTACGTTCGTGCATTACTTCCAT 8 0.2 No Hit CATCAATATCTACTGGAGGGGCTGCGATGAAGGCGATAATAAATACAGAA 8 0.2 No Hit CTCCACGCGGCATTGCTCCGTCAGGCTTTCGCCCATTGCGGAAAATTCCC 8 0.2 No Hit CGCGACCTTGACTATCAACTACAGATTGGTTGAAATTGAATCCATTTAGG 8 0.2 No Hit GGGGCTGCGATGAAGGCGATAATAAATACAGAAGTTGCGGTCAATAAGGT 8 0.2 No Hit ACCAGCTACACCTAACATGTGAAATGGATGCATAAGGATGTTGTGCTCTG 7 0.17500000000000002 No Hit CAGAGAAGCTTCCTTGACCAATAGGGTAAATCAAGAAAACAGCAGTCGCA 7 0.17500000000000002 No Hit GGAAAAACCTTATCCATTAATAGCGGGAACTTCAAGAGCAGCTAGATCTA 7 0.17500000000000002 No Hit CGAGAGTTGTTGAAACTAGCATATTGGAAGATTAATCGGCCAAAATAACC 7 0.17500000000000002 No Hit GACCACCATTGTATAACCATTCATCAACGGATGCAGCTTCCCAAATTGGG 7 0.17500000000000002 No Hit TGATGATATCAGCCCAAGTATTAATAACGCGACCTTGACTATCAACTACA 7 0.17500000000000002 No Hit ACCTTATCCATTAATAGCGGGAACTTCAAGAGCAGCTAGATCTAGAGGGA 7 0.17500000000000002 No Hit AGCGGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTAC 7 0.17500000000000002 No Hit CTAGCATATTGGAAGATTAATCGGCCAAAATAACCATGAGCAGCCACAAT 7 0.17500000000000002 No Hit CAGCAGTCGCAGCTGCAACAGGAGCTGAATATGCAACAGCAATCCAAGGG 7 0.17500000000000002 No Hit CCATGAGCAGCCACAATATTATAAGTCTCTTCCTCTTGACCAAATTTGTA 7 0.17500000000000002 No Hit CCTCATTAGCAGATTCATTTTCAGTAGTTTCCCTGATCAAACTAGAGGTT 7 0.17500000000000002 No Hit CGCCGAAAACACCAGCTACACCTAACATGTGAAATGGATGCATAAGGATG 7 0.17500000000000002 No Hit GCGATAATAAATACAGAAGTTGCGGTCAATAAGGTAGGGATCATCAAAAC 6 0.15 No Hit ACCAGATATTCCTAAAGGCATACCATCAGAGAAGCTTCCTTGACCAATAG 6 0.15 No Hit GTCTCTTCCTCTTGACCAAATTTGTAACCCTCATTAGCAGATTCATTTTC 6 0.15 No Hit GTCGGTTTCGGGTACAGGTACCCTTTTGTTGAAGGTCGTTCGAGCTTTTC 6 0.15 No Hit TCTGCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTCCTAAAGG 6 0.15 No Hit ATCCGATTGCCGCAGAAGTAGGAATAATAGCACCAGAGATAATATTGTTT 6 0.15 No Hit CCATATAACAAGCTACACCAAGTAAGAAGTGTAGAACAATTAGCTCATAA 6 0.15 No Hit GCATTACGTTCGTGCATTACTTCCATACCAAGATTAGCACGGTTGATGAT 6 0.15 No Hit TCTTGACCAAATTTGTAACCCTCATTAGCAGATTCATTTTCAGTAGTTTC 6 0.15 No Hit GCATATTGGAAGATTAATCGGCCAAAATAACCATGAGCAGCCACAATATT 6 0.15 No Hit GCTGCGATGAAGGCGATAATAAATACAGAAGTTGCGGTCAATAAGGTAGG 6 0.15 No Hit CCAAGTATTAATAACGCGACCTTGACTATCAACTACAGATTGGTTGAAAT 6 0.15 No Hit GTGCTAGTTATCCAGTTGCAGAAGCGACCCCACAGGCTTGTACTTTCGCG 6 0.15 No Hit CATAAGGATGTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAAAGTAC 6 0.15 No Hit CTCATTAGCAGATTCATTTTCAGTAGTTTCCCTGATCAAACTAGAGGTTA 6 0.15 No Hit AGCACGGTTGATGATATCAGCCCAAGTATTAATAACGCGACCTTGACTAT 6 0.15 No Hit CCGGGAACGGATTCACCGCCGTATGGCTGACCGGCGATTACTAGCGATTC 6 0.15 No Hit GGTAAATCAAGAAAACAGCAGTCGCAGCTGCAACAGGAGCTGAATATGCA 6 0.15 No Hit CTCCACCGCTTGTGCGGGCCCCCGTCAATTCCTTTGAGTTTCATTCTTGC 5 0.125 No Hit CCTCAGCCTACGGGGTATTAGCAACCGTTTCCAGTTGTTGTTCCCCTCCC 5 0.125 No Hit GGCGCATACCCAAACGGAAACTAAGTTCCCACTCACGACCCATATAACAA 5 0.125 No Hit GTACAAAGGGCAGGGACGTAGTCAACGCGAGCTGATGACTCGCGCTTACT 5 0.125 No Hit GTTGCGGTCAATAAGGTAGGGATCATCAAAACACCGAACCATCCGATGTA 5 0.125 No Hit CCCTCATTAGCAGATTCATTTTCAGTAGTTTCCCTGATCAAACTAGAGGT 5 0.125 No Hit TTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCATTAATA 5 0.125 No Hit ACGCGACCTTGACTATCAACTACAGATTGGTTGAAATTGAATCCATTTAG 5 0.125 No Hit GGCCCATATAACAAGCTACACCAAGTAAGAAGTGTAGAACAATTAGCTCA 5 0.125 No Hit GTAGGGATCATCAAAACACCGAACCATCCGATGTAAAGACGATTTTCAGT 5 0.125 No Hit CAGAGATAATATTGTTTCCATAAAGTAAAGAACCAGAAACAGGCTCGCGA 5 0.125 No Hit CCAGAGATAATATTGTTTCCATAAAGTAAAGAACCAGAAACAGGCTCGCG 5 0.125 No Hit AGCAGCCACAATATTATAAGTCTCTTCCTCTTGACCAAATTTGTAACCCT 5 0.125 No Hit TAGCGGAAAAACCTTATCCATTAATAGCGGGAACTTCAAGAGCAGCTAGA 5 0.125 No Hit GGACCACCATTGTATAACCATTCATCAACGGATGCAGCTTCCCAAATTGG 5 0.125 No Hit CCATTAATAGCGGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTG 5 0.125 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0125 0.0 0.0 0.0 0.0 66-67 0.05 0.0 0.0 0.0 0.0 68-69 0.05 0.0 0.0 0.0 0.0 70-71 0.075 0.0 0.0 0.0 0.0 72-73 0.125 0.0 0.0 0.0 0.0 74-75 0.175 0.0 0.0 0.0 0.0 76-77 0.2375 0.0 0.0 0.0 0.0 78-79 0.275 0.0 0.0 0.0 0.0 80-81 0.2875 0.0 0.0 0.0 0.0 82-83 0.4 0.0 0.0 0.0 0.0 84-85 0.4375 0.0 0.0 0.0 0.0 86-87 0.5 0.0 0.0 0.0 0.0 88-89 0.6125 0.0 0.0 0.0 0.0 90-91 0.775 0.0 0.0 0.0 0.0 92-93 0.85 0.0 0.0 0.0 0.0 94-95 1.075 0.0 0.0 0.0 0.0 96-97 1.4625 0.0 0.0 0.0 0.0 98-99 1.75 0.0 0.0 0.0 0.0 100-101 1.975 0.0 0.0 0.0 0.0 102-103 2.2874999999999996 0.0 0.0 0.0 0.0 104-105 2.6125 0.0 0.0 0.0 0.0 106-107 2.9000000000000004 0.0 0.0 0.0 0.0 108-109 3.1125 0.0 0.0 0.0 0.0 110-111 3.4625000000000004 0.0 0.0 0.0 0.0 112-113 3.7750000000000004 0.0 0.0 0.0 0.0 114-115 4.1875 0.0 0.0 0.0 0.0 116-117 4.7 0.0 0.0 0.0 0.0 118-119 4.875 0.0 0.0 0.0 0.0 120-121 5.25 0.0 0.0 0.0 0.0 122-123 5.5375 0.0 0.0 0.0 0.0 124-125 5.8625 0.0 0.0 0.0 0.0 126-127 6.362500000000001 0.0 0.0 0.0 0.0 128-129 6.6625 0.0 0.0 0.0 0.0 130-131 7.1375 0.0 0.0 0.0 0.0 132-133 7.4625 0.0 0.0 0.0 0.0 134-135 7.85 0.0 0.0 0.0 0.0 136-137 8.3375 0.0 0.0 0.0 0.0 138-139 8.7 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCTTTCT 10 0.006635205 146.3924 1 TAGCGGA 35 0.0031862527 62.739605 3 TTAGCGG 35 0.0031862527 62.739605 2 AGCGGAA 40 0.0054075113 54.89715 4 GTTAGCG 40 0.0054075113 54.89715 1 GCGGAAA 45 0.00861702 48.797466 5 TCGGAAG 40 0.007677306 18.1156 125-129 ACACGTC 40 0.0077896207 18.07031 135-139 TCTGAAC 40 0.0077896207 18.07031 140-144 >>END_MODULE SRR6941563 read2 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR6941563_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 33.76375 35.0 35.0 35.0 32.0 35.0 2 34.08275 35.0 35.0 35.0 33.0 35.0 3 34.2595 35.0 35.0 35.0 33.0 35.0 4 34.16525 35.0 35.0 35.0 33.0 35.0 5 34.283 35.0 35.0 35.0 33.0 35.0 6 38.90675 40.0 40.0 40.0 38.0 40.0 7 38.84125 40.0 40.0 40.0 38.0 40.0 8 39.05525 40.0 40.0 40.0 38.0 40.0 9 39.0565 40.0 40.0 40.0 39.0 40.0 10-14 39.0209 40.0 40.0 40.0 38.6 40.0 15-19 39.1247 40.0 40.0 40.0 39.0 40.0 20-24 39.1023 40.0 40.0 40.0 39.0 40.0 25-29 39.0656 40.0 40.0 40.0 39.0 40.0 30-34 39.0141 40.0 40.0 40.0 38.6 40.0 35-39 38.905899999999995 40.0 39.8 40.0 38.2 40.0 40-44 38.931599999999996 40.0 40.0 40.0 38.2 40.0 45-49 38.87519999999999 40.0 39.8 40.0 38.2 40.0 50-54 38.7073 40.0 39.0 40.0 37.6 40.0 55-59 38.657 40.0 39.2 40.0 37.2 40.0 60-64 38.6437 40.0 39.0 40.0 36.4 40.0 65-69 38.67215 40.0 39.2 40.0 37.0 40.0 70-74 38.50135 40.0 39.0 40.0 36.2 40.0 75-79 38.51805 40.0 39.0 40.0 36.4 40.0 80-84 38.54465 40.0 39.0 40.0 36.6 40.0 85-89 38.72709999999999 40.0 39.0 40.0 37.0 40.0 90-94 38.6375 40.0 39.0 40.0 36.8 40.0 95-99 38.419349999999994 40.0 39.0 40.0 36.0 40.0 100-104 37.1349 38.6 37.6 39.2 33.6 39.4 105-109 38.4348 40.0 39.0 40.0 36.0 40.0 110-114 38.49175 40.0 39.0 40.0 36.6 40.0 115-119 38.347300000000004 40.0 39.0 40.0 35.8 40.0 120-124 38.2616 40.0 39.0 40.0 35.6 40.0 125-129 38.34415 40.0 39.0 40.0 36.0 40.0 130-134 38.13365 40.0 39.0 40.0 35.8 40.0 135-139 38.073899999999995 40.0 39.0 40.0 35.4 40.0 140-144 37.6077 40.0 39.0 40.0 34.2 40.0 145-149 37.17695 40.0 38.6 40.0 33.0 40.0 150-151 34.077625 38.0 34.5 39.5 23.0 40.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5.0 3 2.0 4 1.0 5 0.0 6 1.0 7 0.0 8 0.0 9 0.0 10 0.0 11 2.0 12 0.0 13 0.0 14 1.0 15 1.0 16 1.0 17 3.0 18 2.0 19 1.0 20 5.0 21 4.0 22 7.0 23 5.0 24 7.0 25 6.0 26 18.0 27 16.0 28 30.0 29 25.0 30 33.0 31 35.0 32 48.0 33 50.0 34 63.0 35 82.0 36 96.0 37 181.0 38 421.0 39 2848.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.40991902834008 19.331983805668017 13.739878542510123 26.518218623481783 2 25.464124435524337 22.75464124435524 36.026091319618665 15.755143000501755 3 18.8 26.674999999999997 37.574999999999996 16.950000000000003 4 24.4 29.45 27.075 19.075 5 25.724999999999998 29.75 26.450000000000003 18.075 6 21.375 34.5 26.474999999999998 17.65 7 18.075 21.5 42.95 17.474999999999998 8 22.3 23.474999999999998 31.35 22.875 9 23.549999999999997 20.549999999999997 34.1 21.8 10-14 22.695 25.77 32.145 19.39 15-19 23.32 24.785 31.72 20.175 20-24 23.465 25.430000000000003 31.785000000000004 19.32 25-29 24.310000000000002 24.955 31.014999999999997 19.72 30-34 24.035 24.345 31.85 19.77 35-39 24.735 24.279999999999998 31.255 19.73 40-44 23.095 25.53 30.9 20.474999999999998 45-49 23.244999999999997 26.06 30.520000000000003 20.175 50-54 23.544999999999998 24.755 31.53 20.169999999999998 55-59 22.770000000000003 27.029999999999998 29.615000000000002 20.585 60-64 23.580000000000002 24.945 30.990000000000002 20.485 65-69 23.405 25.330000000000002 30.380000000000003 20.885 70-74 23.98 25.314999999999998 31.2 19.505 75-79 23.849999999999998 25.03 30.3 20.82 80-84 23.724999999999998 23.915 32.79 19.57 85-89 24.915000000000003 25.009999999999998 30.495 19.580000000000002 90-94 23.695 24.795 30.130000000000003 21.38 95-99 23.849999999999998 24.91 30.570000000000004 20.669999999999998 100-104 23.74 25.395 30.8 20.064999999999998 105-109 25.55 24.099999999999998 30.409999999999997 19.939999999999998 110-114 24.41 24.834999999999997 31.319999999999997 19.435 115-119 25.135 24.905 30.354999999999997 19.605 120-124 23.695 25.540000000000003 29.955 20.810000000000002 125-129 24.610000000000003 25.4 29.985 20.005 130-134 24.0 25.28 30.264999999999997 20.455000000000002 135-139 24.07 25.669999999999998 30.59 19.67 140-144 25.22 25.795 29.95 19.035 145-149 24.075 25.064999999999998 31.305 19.555 150-151 23.815492604662822 25.156680872399097 31.210829781900223 19.816996741037855 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2.0 1 1.5 2 0.5 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 1.0 13 1.5 14 0.5 15 0.0 16 0.0 17 1.0 18 1.5 19 0.5 20 0.5 21 1.0 22 1.5 23 3.0 24 7.0 25 10.0 26 11.0 27 13.5 28 19.5 29 28.0 30 28.0 31 24.5 32 31.0 33 51.5 34 75.0 35 86.0 36 103.0 37 156.5 38 201.5 39 215.0 40 235.5 41 260.5 42 232.5 43 225.0 44 261.0 45 238.5 46 209.5 47 169.0 48 118.0 49 92.0 50 82.5 51 80.5 52 62.5 53 54.5 54 73.0 55 86.5 56 82.0 57 61.0 58 43.0 59 40.5 60 45.0 61 47.0 62 40.5 63 29.0 64 16.0 65 7.5 66 3.5 67 5.0 68 6.0 69 5.5 70 3.0 71 2.0 72 2.0 73 1.5 74 1.0 75 0.0 76 0.0 77 1.0 78 1.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 1.2 2 0.35000000000000003 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.27499999999999997 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.64999999999999 #Duplication Level Percentage of deduplicated Percentage of total 1 81.16520351157223 50.849999999999994 2 8.060654429369512 10.100000000000001 3 4.26975259377494 8.025 4 1.596169193934557 4.0 5 1.1971268954509178 3.75 6 0.9177972865123704 3.45 7 0.5187549880287311 2.275 8 0.4788507581803671 2.4 9 0.1596169193934557 0.8999999999999999 >10 1.6360734237829209 14.249999999999998 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source ATTATCTATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAAT 32 0.8 No Hit TATCTATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTT 28 0.7000000000000001 No Hit CTATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAA 23 0.575 No Hit GTGGGAACTTAGTTTCCGTTTGGGTATGCGCCCTTGGATTGCTGTTGCAT 20 0.5 No Hit GTTTCAACAACTCTCGTTCTTTACACTTCTTCTTGGCTGCTTGGCCTGTA 20 0.5 No Hit GTTCGATCCTGGCTCAGGATGAACGCTGGCGGCATGCTTAACACATGCAA 18 0.44999999999999996 No Hit GGAAGCTTCTCTGATGGTATGCCTTTAGGAATATCTGGTACTTTCAACTT 18 0.44999999999999996 No Hit GTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGATCTTACCATGAC 18 0.44999999999999996 No Hit GTTATTTTGGCCGATTAATCTTCCAATATGCTAGTTTCAACAACTCTCGT 17 0.42500000000000004 No Hit CAACTCTCGTTCTTTACACTTCTTCTTGGCTGCTTGGCCTGTAGTAGGAA 16 0.4 No Hit GCACAACATCCTTATGCATCCATTTCACATGTTAGGTGTAGCTGGTGTTT 16 0.4 No Hit GCTAGTTTCAACAACTCTCGTTCTTTACACTTCTTCTTGGCTGCTTGGCC 15 0.375 No Hit GTTAGGTGTAGCTGGTGTTTTCGGCGGTTCCCTATTCAGTGCTATGCATG 15 0.375 No Hit ATCTATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTG 15 0.375 No Hit GCTTGTTATATGGGTCGTGAGTGGGAACTTAGTTTCCGTTTGGGTATGCG 14 0.35000000000000003 No Hit CAATGGTGGTCCTTATGAGCTAATTGTTCTACACTTCTTACTTGGTGTAG 14 0.35000000000000003 No Hit CTTTTACCCAATTTGGGAAGCTGCATCCGTTGATGAATGGTTATACAATG 13 0.325 No Hit GGAATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCTAAA 13 0.325 No Hit GTAGCTTGTTATATGGGTCGTGAGTGGGAACTTAGTTTCCGTTTGGGTAT 13 0.325 No Hit GGAAGAGACTTATAATATTGTGGCTGCTCATGGTTATTTTGGCCGATTAA 12 0.3 No Hit GGTACTTTCAACTTTATGATTGTATTCCAGGCAGAGCACAACATCCTTAT 12 0.3 No Hit GCTAATTGTTCTACACTTCTTACTTGGTGTAGCTTGTTATATGGGTCGTG 11 0.27499999999999997 No Hit GGGAAACTACTGAAAATGAATCTGCTAATGAGGGTTACAAATTTGGTCAA 11 0.27499999999999997 No Hit GTTCCTTGGTAACCTCTAGTTTGATCAGGGAAACTACTGAAAATGAATCT 11 0.27499999999999997 No Hit ATTTGGTCAAGAGGAAGAGACTTATAATATTGTGGCTGCTCATGGTTATT 11 0.27499999999999997 No Hit GCATGGTTCCTTGGTAACCTCTAGTTTGATCAGGGAAACTACTGAAAATG 11 0.27499999999999997 No Hit GAGGAAGAGACTTATAATATTGTGGCTGCTCATGGTTATTTTGGCCGATT 11 0.27499999999999997 No Hit CTTCCAATATGCTAGTTTCAACAACTCTCGTTCTTTACACTTCTTCTTGG 11 0.27499999999999997 No Hit CTGAAAATGAATCTGCTAATGAGGGTTACAAATTTGGTCAAGAGGAAGAG 11 0.27499999999999997 No Hit ATCGGATTGCACTTTTACCCAATTTGGGAAGCTGCATCCGTTGATGAATG 10 0.25 No Hit CTGGTACTTTCAACTTTATGATTGTATTCCAGGCAGAGCACAACATCCTT 10 0.25 No Hit CCTAAATGGATTCAATTTCAACCAATCTGTAGTTGATAGTCAAGGTCGCG 10 0.25 No Hit GTTTTGATGATCCCTACCTTATTGACCGCAACTTCTGTATTTATTATCGC 10 0.25 No Hit CTAGCACTGAAAATCGTCTTTACATCGGATGGTTCGGTGTTTTGATGATC 10 0.25 No Hit ATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCA 10 0.25 No Hit GCCTTTAGGAATATCTGGTACTTTCAACTTTATGATTGTATTCCAGGCAG 10 0.25 No Hit ATTCAGTGCTATGCATGGTTCCTTGGTAACCTCTAGTTTGATCAGGGAAA 10 0.25 No Hit GTTTCTGGTTCTTTACTTTATGGAAACAATATTATCTCTGGTGCTATTAT 10 0.25 No Hit GTTGATGAATGGTTATACAATGGTGGTCCTTATGAGCTAATTGTTCTACA 10 0.25 No Hit GCTGCATCCGTTGATGAATGGTTATACAATGGTGGTCCTTATGAGCTAAT 10 0.25 No Hit CAGGGAAACTACTGAAAATGAATCTGCTAATGAGGGTTACAAATTTGGTC 10 0.25 No Hit GGATGGTTCGGTGTTTTGATGATCCCTACCTTATTGACCGCAACTTCTGT 9 0.22499999999999998 No Hit GTTGCATATTCAGCTCCTGTTGCAGCTGCGACTGCTGTTTTCTTGATTTA 9 0.22499999999999998 No Hit TCTATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGA 9 0.22499999999999998 No Hit CAACATCCTTATGCATCCATTTCACATGTTAGGTGTAGCTGGTGTTTTCG 9 0.22499999999999998 No Hit GTCGTGAGTGGGAACTTAGTTTCCGTTTGGGTATGCGCCCTTGGATTGCT 8 0.2 No Hit GGAAACTACTGAAAATGAATCTGCTAATGAGGGTTACAAATTTGGTCAAG 8 0.2 No Hit CATCCGTTGATGAATGGTTATACAATGGTGGTCCTTATGAGCTAATTGTT 8 0.2 No Hit GAGGGTTACAAATTTGGTCAAGAGGAAGAGACTTATAATATTGTGGCTGC 8 0.2 No Hit CGTTGATGAATGGTTATACAATGGTGGTCCTTATGAGCTAATTGTTCTAC 8 0.2 No Hit GGTTATTTTGGCCGATTAATCTTCCAATATGCTAGTTTCAACAACTCTCG 8 0.2 No Hit AAACAATATTATCTCTGGTGCTATTATTCCTACTTCTGCGGCAATCGGAT 8 0.2 No Hit AGTCAAGGTCGCGTTATTAATACTTGGGCTGATATCATCAACCGTGCTAA 8 0.2 No Hit AGGGAAACTACTGAAAATGAATCTGCTAATGAGGGTTACAAATTTGGTCA 8 0.2 No Hit GAAGAGACTTATAATATTGTGGCTGCTCATGGTTATTTTGGCCGATTAAT 8 0.2 No Hit GTTCAACCTAAATGGATTCAATTTCAACCAATCTGTAGTTGATAGTCAAG 8 0.2 No Hit GAGCACAACATCCTTATGCATCCATTTCACATGTTAGGTGTAGCTGGTGT 8 0.2 No Hit GGAATATCTGGTACTTTCAACTTTATGATTGTATTCCAGGCAGAGCACAA 7 0.17500000000000002 No Hit CTCTAGTTTGATCAGGGAAACTACTGAAAATGAATCTGCTAATGAGGGTT 7 0.17500000000000002 No Hit CGCAGCCCCTCCAGTAGATATTGATGGTATTCGCGAGCCTGTTTCTGGTT 7 0.17500000000000002 No Hit ATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAA 7 0.17500000000000002 No Hit CTTCTTGGCTGCTTGGCCTGTAGTAGGAATCTGGTTCACTGCTTTAGGTA 7 0.17500000000000002 No Hit GTTATACAATGGTGGTCCTTATGAGCTAATTGTTCTACACTTCTTACTTG 7 0.17500000000000002 No Hit GTAGGAATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCT 7 0.17500000000000002 No Hit GGGAACTTAGTTTCCGTTTGGGTATGCGCCCTTGGATTGCTGTTGCATAT 7 0.17500000000000002 No Hit CGAAAGTACAAGCCTGTGGGGTCGCTTCTGCAACTGGATAACTAGCACTG 7 0.17500000000000002 No Hit GCTGCGACTGCTGTTTTCTTGATTTACCCTATTGGTCAAGGAAGCTTCTC 7 0.17500000000000002 No Hit GTCAAGGTCGCGTTATTAATACTTGGGCTGATATCATCAACCGTGCTAAT 7 0.17500000000000002 No Hit GTTTGATCAGGGAAACTACTGAAAATGAATCTGCTAATGAGGGTTACAAA 7 0.17500000000000002 No Hit GTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGATCTTACCATGACTG 7 0.17500000000000002 No Hit AGCACAACATCCTTATGCATCCATTTCACATGTTAGGTGTAGCTGGTGTT 6 0.15 No Hit GTCCTTATGAGCTAATTGTTCTACACTTCTTACTTGGTGTAGCTTGTTAT 6 0.15 No Hit CCAATATGCTAGTTTCAACAACTCTCGTTCTTTACACTTCTTCTTGGCTG 6 0.15 No Hit TGTAGCTTGTTATATGGGTCGTGAGTGGGAACTTAGTTTCCGTTTGGGTA 6 0.15 No Hit CCAAGATCTTACCATGACTGCAATTTTAGAGAGACGCGAAAGTACAAGCC 6 0.15 No Hit ATCGCCTTCATCGCAGCCCCTCCAGTAGATATTGATGGTATTCGCGAGCC 6 0.15 No Hit GGCTGCTTGGCCTGTAGTAGGAATCTGGTTCACTGCTTTAGGTATTAGTA 6 0.15 No Hit TTATAATATTGTGGCTGCTCATGGTTATTTTGGCCGATTAATCTTCCAAT 6 0.15 No Hit GCTGTTTTCTTGATTTACCCTATTGGTCAAGGAAGCTTCTCTGATGGTAT 6 0.15 No Hit CTTGCAATTTGAATAAACCAAGATCTTACCATGACTGCAATTTTAGAGAG 6 0.15 No Hit ATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATA 6 0.15 No Hit GTTGATAGTCAAGGTCGCGTTATTAATACTTGGGCTGATATCATCAACCG 6 0.15 No Hit CTTAGTTTCCGTTTGGGTATGCGCCCTTGGATTGCTGTTGCATATTCAGC 6 0.15 No Hit GGCTTGCGGTGGATACCTAGGCACCCAGAGACGAGGAAGGGCGTAGCAAG 6 0.15 No Hit TATGCCTTTAGGAATATCTGGTACTTTCAACTTTATGATTGTATTCCAGG 6 0.15 No Hit CCCTATTCAGTGCTATGCATGGTTCCTTGGTAACCTCTAGTTTGATCAGG 6 0.15 No Hit CAGTGCTATGCATGGTTCCTTGGTAACCTCTAGTTTGATCAGGGAAACTA 6 0.15 No Hit TGTAGCTGGTGTTTTCGGCGGTTCCCTATTCAGTGCTATGCATGGTTCCT 6 0.15 No Hit GTTTCCGTTTGGGTATGCGCCCTTGGATTGCTGTTGCATATTCAGCTCCT 6 0.15 No Hit ATTGTATTCCAGGCAGAGCACAACATCCTTATGCATCCATTTCACATGTT 6 0.15 No Hit CGGATGGTTCGGTGTTTTGATGATCCCTACCTTATTGACCGCAACTTCTG 6 0.15 No Hit CACATGTTAGGTGTAGCTGGTGTTTTCGGCGGTTCCCTATTCAGTGCTAT 6 0.15 No Hit AGAACCTTACCAGGGCTTGACATGCCGCGAATCCTCTTGAAAGAGAGGGG 6 0.15 No Hit GCTCATGGTTATTTTGGCCGATTAATCTTCCAATATGCTAGTTTCAACAA 5 0.125 No Hit TGATGGTATGCCTTTAGGAATATCTGGTACTTTCAACTTTATGATTGTAT 5 0.125 No Hit CATGGTTATTTTGGCCGATTAATCTTCCAATATGCTAGTTTCAACAACTC 5 0.125 No Hit ATTATTCCTACTTCTGCGGCAATCGGATTGCACTTTTACCCAATTTGGGA 5 0.125 No Hit GTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCTAAATGGATTCA 5 0.125 No Hit GAATCTGCTAATGAGGGTTACAAATTTGGTCAAGAGGAAGAGACTTATAA 5 0.125 No Hit GCTTGGGAGTCCTTGCAATTTGAATAAACCAAGATCTTACCATGACTGCA 5 0.125 No Hit CTTATGAGCTAATTGTTCTACACTTCTTACTTGGTGTAGCTTGTTATATG 5 0.125 No Hit GGGTTACAAATTTGGTCAAGAGGAAGAGACTTATAATATTGTGGCTGCTC 5 0.125 No Hit TTGCAATTTGAATAAACCAAGATCTTACCATGACTGCAATTTTAGAGAGA 5 0.125 No Hit ATCGTCTTTACATCGGATGGTTCGGTGTTTTGATGATCCCTACCTTATTG 5 0.125 No Hit GAGACTTATAATATTGTGGCTGCTCATGGTTATTTTGGCCGATTAATCTT 5 0.125 No Hit TGATGATCCCTACCTTATTGACCGCAACTTCTGTATTTATTATCGCCTTC 5 0.125 No Hit CTTTAGGAATATCTGGTACTTTCAACTTTATGATTGTATTCCAGGCAGAG 5 0.125 No Hit CATGACTGCAATTTTAGAGAGACGCGAAAGTACAAGCCTGTGGGGTCGCT 5 0.125 No Hit GTTACAAATTTGGTCAAGAGGAAGAGACTTATAATATTGTGGCTGCTCAT 5 0.125 No Hit GTTATATGGGTCGTGAGTGGGAACTTAGTTTCCGTTTGGGTATGCGCCCT 5 0.125 No Hit CGCGAGCCTGTTTCTGGTTCTTTACTTTATGGAAACAATATTATCTCTGG 5 0.125 No Hit CCTATTCAGTGCTATGCATGGTTCCTTGGTAACCTCTAGTTTGATCAGGG 5 0.125 No Hit ATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCTAAATGG 5 0.125 No Hit AACTCTCGTTCTTTACACTTCTTCTTGGCTGCTTGGCCTGTAGTAGGAAT 5 0.125 No Hit TTCCAATATGCTAGTTTCAACAACTCTCGTTCTTTACACTTCTTCTTGGC 5 0.125 No Hit CCCAATTTGGGAAGCTGCATCCGTTGATGAATGGTTATACAATGGTGGTC 5 0.125 No Hit ATCTTCCAATATGCTAGTTTCAACAACTCTCGTTCTTTACACTTCTTCTT 5 0.125 No Hit CCTTACCAGGGCTTGACATGCCGCGAATCCTCTTGAAAGAGAGGGGTGCC 5 0.125 No Hit CGATCCTGGCTCAGGATGAACGCTGGCGGCATGCTTAACACATGCAAGTC 5 0.125 No Hit GAAACAATGACGGTATCTGAGGAATAAGCATCGGCTAACTCTGTGCCAGC 5 0.125 No Hit GAATGGTTATACAATGGTGGTCCTTATGAGCTAATTGTTCTACACTTCTT 5 0.125 No Hit GATCCCTACCTTATTGACCGCAACTTCTGTATTTATTATCGCCTTCATCG 5 0.125 No Hit GAAGCTTCTCTGATGGTATGCCTTTAGGAATATCTGGTACTTTCAACTTT 5 0.125 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0125 0.0 0.0 0.0 0.0 66-67 0.05 0.0 0.0 0.0 0.0 68-69 0.05 0.0 0.0 0.0 0.0 70-71 0.075 0.0 0.0 0.0 0.0 72-73 0.125 0.0 0.0 0.0 0.0 74-75 0.175 0.0 0.0 0.0 0.0 76-77 0.2375 0.0 0.0 0.0 0.0 78-79 0.275 0.0 0.0 0.0 0.0 80-81 0.2875 0.0 0.0 0.0 0.0 82-83 0.4 0.0 0.0 0.0 0.0 84-85 0.4375 0.0 0.0 0.0 0.0 86-87 0.5 0.0 0.0 0.0 0.0 88-89 0.6125 0.0 0.0 0.0 0.0 90-91 0.775 0.0 0.0 0.0 0.0 92-93 0.85 0.0 0.0 0.0 0.0 94-95 1.075 0.0 0.0 0.0 0.0 96-97 1.4625 0.0 0.0 0.0 0.0 98-99 1.75 0.0 0.0 0.0 0.0 100-101 1.975 0.0 0.0 0.0 0.0 102-103 2.3125 0.0 0.0 0.0 0.0 104-105 2.65 0.0 0.0 0.0 0.0 106-107 2.95 0.0 0.0 0.0 0.0 108-109 3.1625 0.0 0.0 0.0 0.0 110-111 3.5125 0.0 0.0 0.0 0.0 112-113 3.825 0.0 0.0 0.0 0.0 114-115 4.225 0.0 0.0 0.0 0.0 116-117 4.675 0.0 0.0 0.0 0.0 118-119 4.8375 0.0 0.0 0.0 0.0 120-121 5.2 0.0 0.0 0.0 0.0 122-123 5.487500000000001 0.0 0.0 0.0 0.0 124-125 5.8125 0.0 0.0 0.0 0.0 126-127 6.3125 0.0 0.0 0.0 0.0 128-129 6.612500000000001 0.0 0.0 0.0 0.0 130-131 7.075 0.0 0.0 0.0 0.0 132-133 7.3875 0.0 0.0 0.0 0.0 134-135 7.824999999999999 0.0 0.0 0.0 0.0 136-137 8.3125 0.0 0.0 0.0 0.0 138-139 8.675 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGAAAAG 10 0.0065806094 146.79747 1 GTCGTGT 40 0.007666461 18.120312 135-139 TCGGAAG 40 0.007666461 18.120312 125-129 GTAGGGA 40 0.007666461 18.120312 140-144 >>END_MODULE Read 1184247 spots for SRR6941563.sra Written 1184247 spots for SRR6941563.sra Read 1184247 spots for SRR6941563.sra Written 1184247 spots for SRR6941563.sra Read 1184247 spots for SRR6941563.sra Written 1184247 spots for SRR6941563.sra Read 1184247 spots for SRR6941563.sra Written 1184247 spots for SRR6941563.sra Read 1184247 spots for SRR6941563.sra Written 1184247 spots for SRR6941563.sra Read 1184262 spots for SRR6941563.sra Written 1184262 spots for SRR6941563.sra Read 1184247 spots for SRR6941563.sra Written 1184247 spots for SRR6941563.sra Read 1184247 spots for SRR6941563.sra Written 1184247 spots for SRR6941563.sra Read 1184247 spots for SRR6941563.sra Written 1184247 spots for SRR6941563.sra Read 1184247 spots for SRR6941563.sra Written 1184247 spots for SRR6941563.sra Read 1184247 spots for SRR6941563.sra Written 1184247 spots for SRR6941563.sra Read 1184247 spots for SRR6941563.sra Written 1184247 spots for SRR6941563.sra Read 1184247 spots for SRR6941563.sra Written 1184247 spots for SRR6941563.sra Read 1184247 spots for SRR6941563.sra Written 1184247 spots for SRR6941563.sra Read 1184247 spots for SRR6941563.sra Written 1184247 spots for SRR6941563.sra Read 1184247 spots for SRR6941563.sra Written 1184247 spots for SRR6941563.sra Read 1184247 spots for SRR6941563.sra Written 1184247 spots for SRR6941563.sra Read 1184247 spots for SRR6941563.sra Written 1184247 spots for SRR6941563.sra Read 1184247 spots for SRR6941563.sra Written 1184247 spots for SRR6941563.sra Read 1184247 spots for SRR6941563.sra Written 1184247 spots for SRR6941563.sra SRR ids: ['SRR6941563.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_3uq83f0r SRR6941563.sra spots: 23684955 blocks: [[1, 1184247], [1184248, 2368494], [2368495, 3552741], [3552742, 4736988], [4736989, 5921235], [5921236, 7105482], [7105483, 8289729], [8289730, 9473976], [9473977, 10658223], [10658224, 11842470], [11842471, 13026717], [13026718, 14210964], [14210965, 15395211], [15395212, 16579458], [16579459, 17763705], [17763706, 18947952], [18947953, 20132199], [20132200, 21316446], [21316447, 22500693], [22500694, 23684955]] SRR6941563 file size 8004353 SRR6941563 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR6941563 SRR6941563_1.fastq SRR6941563_2.fastq Input file: SRR6941563_1.fastq Paired file: SRR6941563_2.fastq trimmed: SRR6941563-trimmed-pair1.fastq, SRR6941563-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Fri Dec 6 11:26:20 2024 >> started Fri Dec 6 11:26:46 2024 >> done (26.491s) 23684955 read pairs processed; of these: 8403 ( 0.04%) short read pairs filtered out after trimming by size control 11290 ( 0.05%) empty read pairs filtered out after trimming by size control 23665262 (99.92%) read pairs available; of these: 4571681 (19.32%) trimmed read pairs available after processing 19093581 (80.68%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 3 0.00% 19 5 0.00% 20 2 0.00% 21 0 0.00% 22 3 0.00% 23 1 0.00% 24 2 0.00% 25 6 0.00% 26 8 0.00% 27 4 0.00% 28 6 0.00% 29 4 0.00% 30 15 0.00% 31 6 0.00% 32 20 0.00% 33 20 0.00% 34 18 0.00% 35 19 0.00% 36 23 0.00% 37 30 0.00% 38 35 0.00% 39 56 0.00% 40 61 0.00% 41 56 0.00% 42 72 0.00% 43 84 0.00% 44 79 0.00% 45 95 0.00% 46 104 0.00% 47 114 0.00% 48 147 0.00% 49 183 0.00% 50 210 0.00% 51 197 0.00% 52 282 0.00% 53 317 0.00% 54 371 0.00% 55 414 0.00% 56 444 0.00% 57 521 0.00% 58 627 0.00% 59 647 0.00% 60 787 0.00% 61 916 0.00% 62 1206 0.01% 63 1309 0.01% 64 1479 0.01% 65 1732 0.01% 66 1821 0.01% 67 1931 0.01% 68 2364 0.01% 69 2674 0.01% 70 3107 0.01% 71 3820 0.02% 72 4338 0.02% 73 4671 0.02% 74 4855 0.02% 75 5926 0.03% 76 5604 0.02% 77 6762 0.03% 78 6830 0.03% 79 7172 0.03% 80 8386 0.04% 81 9138 0.04% 82 10482 0.04% 83 10576 0.04% 84 11558 0.05% 85 13785 0.06% 86 14281 0.06% 87 15206 0.06% 88 17107 0.07% 89 16940 0.07% 90 19109 0.08% 91 19095 0.08% 92 22057 0.09% 93 22105 0.09% 94 23874 0.10% 95 26562 0.11% 96 24139 0.10% 97 24342 0.10% 98 24735 0.10% 99 25952 0.11% 100 26743 0.11% 101 28515 0.12% 102 31043 0.13% 103 30523 0.13% 104 33096 0.14% 105 35008 0.15% 106 35315 0.15% 107 35642 0.15% 108 35452 0.15% 109 39691 0.17% 110 37689 0.16% 111 42289 0.18% 112 43734 0.18% 113 40893 0.17% 114 44725 0.19% 115 40699 0.17% 116 42694 0.18% 117 40993 0.17% 118 41879 0.18% 119 42325 0.18% 120 43234 0.18% 121 43844 0.19% 122 48142 0.20% 123 50134 0.21% 124 52206 0.22% 125 53223 0.22% 126 48590 0.21% 127 49609 0.21% 128 50999 0.22% 129 57654 0.24% 130 56715 0.24% 131 61476 0.26% 132 61409 0.26% 133 54118 0.23% 134 62452 0.26% 135 58438 0.25% 136 61394 0.26% 137 60515 0.26% 138 67173 0.28% 139 70880 0.30% 140 66058 0.28% 141 81360 0.34% 142 71143 0.30% 143 76506 0.32% 144 73396 0.31% 145 84819 0.36% 146 90409 0.38% 147 100009 0.42% 148 124528 0.53% 149 184912 0.78% 150 1323344 5.59% 151 19093581 80.68% 23665262 reads passed initial QC criterion=sequence-density sequence-density=0.28 sequence-density-rank=1 fanout-score=4.86 fanout-score-rank=20 prefix-density=0.41 prefix-fanout=3.4 sequence=TACTTGTTCAAC criterion=fanout-score sequence-density=0.01 sequence-density-rank=43 fanout-score=72.39 fanout-score-rank=1 prefix-density=0.06 prefix-fanout=6.9 sequence=AAAAAAAAAGGGGGGTAAGGACCCGCTAAGCTCCTACTTTTTCATGTTTCCAATCCGATCCCTCCGATTACTATAGAGATGAACCCAATCCAGAATATGAACCATAAAAGAAAACACCTACTAAACCAATCACAAGAATACCAGTTACCGTACCTATCAGCCAAAGAGGAATTCTTCCAGTAGTATCGGCCATTTCCCCTACTTTCCTCCACATTTTATCAAGTGGTCATGCTAGAGACAAAAACAGTCATGGATAGTTATGTTATAAGGATGGTATCCTTCCAAATGGGATAAGAGAGTTCTTACTACTCTCTTCTTTTCTCTCAATTAAAGAAGTAATTGGAAAACAAAACAGCAAGTACAAAAATGAGTAATAAACCCCAGTATAGACTGGTACGATTCAATTCAACATTTTGTTCATTCGGGTTTGATTGTGTCATAGTTCTATAGTTGGAATTTAGTTTATCGTTGGATGAACTGCATTGCTGATATTGATCCCAAGAAAAAAACA criterion=sequence-density sequence-density=0.29 sequence-density-rank=1 fanout-score=2.00 fanout-score-rank=25 prefix-density=0.29 prefix-fanout=2.0 sequence=GTAGTAATTCTAG criterion=fanout-score sequence-density=0.01 sequence-density-rank=41 fanout-score=737.89 fanout-score-rank=1 prefix-density=3.87 prefix-fanout=1.0 sequence=AGTAATGCAACTATGAATCTCATGGAGAGTTCGATCCTGGCTCAGGATGAACGCTGGCGGCATGCTTAACACATGCAAGTCGAACGGGAAGTGGTGTTTCCAGTGGCGAACGGGTGAGTAACGCGTAAGAACCTGCCCTTGGGAGGGGAACAACAACTGGAAACGGTTGCTAATACCCCGTAGGCTGAGGAGCAAAAGGAGAAATCCGCCCAAGGAGGGGCTCGCGTCTGATTAGCTAGTTGGTGAGGCAATAGCTTACCAAGGCGATGATCAGTAGCTGGTCCGAGAGGATGATCAGCCACACTGGGACTGAGACACGGCCCAGACTCCTACGGGAGGCAGCAGTGGGGAATTTTCCGCAATGGGCGAAAGCCTGACGGAGCAATGCCGCGTGGAGGTGGAAGGCCTACGGGTCGTCAACTTCTTTTCTCGGAGAAGAAACAATGACGGTATCTGAGGAATAAGCATCGGCTAACTCTGTGCCAGCAGCCGCGGTAAGACAGAGGATGCA SRR6941563 testing PE reads STAR mapping to Ensembl genome Started job on | Dec 06 11:27:18 Started mapping on | Dec 06 11:27:18 Finished on | Dec 06 11:29:08 Mapping speed, Million of reads per hour | 774.50 Number of input reads | 23665262 Average input read length | 294 UNIQUE READS: Uniquely mapped reads number | 14463487 Uniquely mapped reads % | 61.12% Average mapped length | 296.00 Number of splices: Total | 2608885 Number of splices: Annotated (sjdb) | 2328500 Number of splices: GT/AG | 2441830 Number of splices: GC/AG | 31162 Number of splices: AT/AC | 13715 Number of splices: Non-canonical | 122178 Mismatch rate per base, % | 0.25% Deletion rate per base | 0.02% Deletion average length | 1.91 Insertion rate per base | 0.02% Insertion average length | 2.60 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 7833947 % of reads mapped to multiple loci | 33.10% Number of reads mapped to too many loci | 62165 % of reads mapped to too many loci | 0.26% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 4.28% % of reads unmapped: other | 1.23% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 1375451 1375451 1375451 N_multimapping 7833947 7833947 7833947 N_noFeature 6449524 13933944 6710080 N_ambiguous 506415 5227 244353 UnstrandedReadsAssigned:7507548 PositiveStrandReadsAssigned:524316 NegativeStrandReadsAssigned:7509054 Dataset is classified negative stranded MeadianReadLen=151 20thPercentileLength=151 echo kmer=147 SRR6941563 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,972 [index] number of k-mers: 66,720,672 [index] number of equivalence classes: 111,837 [quant] running in paired-end mode [quant] will process pair 1: SRR6941563-trimmed-pair1.fastq SRR6941563-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 23,665,262 reads, 12,481,021 reads pseudoaligned [quant] estimated average fragment length: 234.991 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,034 rounds 52973 SRR6941563.ke.tsv 35125 SRR6941563.se.tsv 88098 total ==> SRR6941563.ke.tsv <== target_id length eff_length est_counts tpm PNS24245 936 702.288 0 0 PNS24247 1044 810.009 14.4212 1.25825 PNS24249 1928 1694.01 6.14785 0.256485 PNS24246 1044 810.009 14.4212 1.25825 PNS24248 1044 810.009 14.4212 1.25825 PNS24244 1471 1237.01 52.5885 3.00449 PNS24243 293 101.49 0 0 KQK14069 1603 1369.01 1688.54 87.1682 KQK14071 474 249.597 44.0304 12.4672 ==> SRR6941563.se.tsv <== BRADI_1g14170v3 2444 BRADI_1g53295v3 57 BRADI_1g59795v3 77 BRADI_1g07683v3 0 BRADI_1g00485v3 0 BRADI_1g20270v3 54 BRADI_1g74790v3 10 BRADI_1g09890v3 0 BRADI_1g77505v3 71 BRADI_1g48960v3 0 SRR6941563 completed mapping pipeline successfully