Starting /dee2/code/volunteer_pipeline.sh SRR6941573 current disk space = 1551348162560 free memory = 1599132564 SRR6941573 SRAfilesize 2e222e3b9fc584f730dde4e0dade1c57 SRR6941573.sra SRR6941573.sra file validated SRR6941573 is paired end SRR6941573 is conventional basespace SRR6941573 read1 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR6941573_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 34.62725 35.0 35.0 35.0 35.0 35.0 2 34.4255 35.0 35.0 35.0 33.0 35.0 3 34.57075 35.0 35.0 35.0 34.0 35.0 4 34.643 35.0 35.0 35.0 35.0 35.0 5 34.4295 35.0 35.0 35.0 34.0 35.0 6 39.26275 40.0 40.0 40.0 39.0 40.0 7 39.26625 40.0 40.0 40.0 39.0 40.0 8 39.186 40.0 40.0 40.0 38.0 40.0 9 39.41625 40.0 40.0 40.0 39.0 40.0 10-14 39.32845 40.0 40.0 40.0 39.0 40.0 15-19 39.14135 40.0 40.0 40.0 38.0 40.0 20-24 39.281850000000006 40.0 40.0 40.0 39.0 40.0 25-29 39.30255 40.0 40.0 40.0 39.0 40.0 30-34 39.18455 40.0 40.0 40.0 38.8 40.0 35-39 39.2495 40.0 40.0 40.0 39.0 40.0 40-44 39.2283 40.0 40.0 40.0 39.0 40.0 45-49 39.19475 40.0 40.0 40.0 38.4 40.0 50-54 39.0947 40.0 40.0 40.0 37.8 40.0 55-59 39.12435 40.0 40.0 40.0 38.2 40.0 60-64 39.06915 40.0 40.0 40.0 38.0 40.0 65-69 39.132799999999996 40.0 40.0 40.0 38.4 40.0 70-74 39.055249999999994 40.0 40.0 40.0 38.0 40.0 75-79 38.94205 40.0 39.8 40.0 37.4 40.0 80-84 38.980399999999996 40.0 39.4 40.0 38.0 40.0 85-89 38.88005 40.0 39.0 40.0 37.0 40.0 90-94 38.938050000000004 40.0 39.0 40.0 37.4 40.0 95-99 38.6923 40.0 39.0 40.0 36.4 40.0 100-104 38.11325000000001 39.4 38.4 39.8 35.4 39.8 105-109 38.78005 40.0 39.0 40.0 36.6 40.0 110-114 38.7658 40.0 39.0 40.0 36.8 40.0 115-119 38.7324 40.0 39.0 40.0 36.6 40.0 120-124 38.63535 40.0 39.0 40.0 36.2 40.0 125-129 38.41205 40.0 39.0 40.0 36.0 40.0 130-134 38.38165 40.0 39.0 40.0 35.8 40.0 135-139 38.265100000000004 40.0 39.0 40.0 35.4 40.0 140-144 38.142250000000004 40.0 39.0 40.0 35.4 40.0 145-149 37.501149999999996 40.0 38.8 40.0 33.2 40.0 150-151 34.699125 38.5 35.0 39.5 24.5 40.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 15 1.0 16 1.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 1.0 23 4.0 24 4.0 25 5.0 26 5.0 27 11.0 28 19.0 29 19.0 30 38.0 31 37.0 32 42.0 33 52.0 34 61.0 35 73.0 36 137.0 37 200.0 38 350.0 39 2940.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 54.42942942942943 7.032032032032031 3.5285285285285286 35.010010010010014 2 22.035597894209076 7.244923539734269 32.765104036099274 37.954374529957384 3 19.400000000000002 14.124999999999998 24.525 41.949999999999996 4 25.85 19.075 20.525 34.55 5 25.15090543259557 26.358148893360163 23.81790744466801 24.673038229376257 6 22.75 29.075 22.825 25.35 7 15.225 25.874999999999996 40.0 18.9 8 18.099999999999998 21.8 33.925 26.174999999999997 9 20.175 19.35 35.175 25.3 10-14 20.875 26.3 27.46 25.365 15-19 22.09 24.285 26.3 27.325 20-24 21.305 26.174999999999997 26.040000000000003 26.479999999999997 25-29 23.375 23.95 27.07 25.605 30-34 22.505 25.035 25.415 27.045 35-39 22.25 23.955000000000002 26.66 27.134999999999998 40-44 21.48 24.83 26.135 27.555000000000003 45-49 22.775000000000002 22.42 26.97 27.834999999999997 50-54 22.67 23.849999999999998 26.424999999999997 27.055 55-59 20.91 24.560000000000002 27.33 27.200000000000003 60-64 22.71 23.98 26.87 26.44 65-69 21.634999999999998 26.11 25.94 26.314999999999998 70-74 21.8 24.935 25.21 28.055000000000003 75-79 22.895 25.615 25.495 25.995 80-84 21.64 27.08 25.205 26.075 85-89 23.03 24.85 25.019999999999996 27.1 90-94 22.73 25.629999999999995 24.73 26.91 95-99 22.38 25.985000000000003 24.310000000000002 27.325 100-104 22.68 25.46 24.87 26.99 105-109 21.349999999999998 25.1 25.31 28.24 110-114 22.15 25.555 25.509999999999998 26.784999999999997 115-119 21.935 25.845000000000002 24.845 27.375 120-124 22.745 26.729999999999997 23.1 27.425 125-129 22.025 26.105 24.240000000000002 27.63 130-134 23.43 24.38 23.485 28.705000000000002 135-139 22.15 25.855 23.990000000000002 28.005000000000003 140-144 23.98 25.215 23.974999999999998 26.83 145-149 22.345000000000002 26.119999999999997 23.585 27.950000000000003 150-151 22.152612454579625 25.798772083698786 23.380528755795012 28.668086705926576 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.5 12 0.5 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.5 23 2.0 24 3.0 25 1.5 26 1.0 27 3.0 28 7.0 29 8.0 30 10.5 31 14.5 32 15.0 33 20.0 34 21.5 35 22.5 36 44.0 37 84.5 38 125.0 39 151.5 40 155.5 41 157.5 42 119.5 43 93.5 44 110.0 45 118.5 46 120.5 47 111.0 48 100.0 49 89.5 50 100.0 51 123.5 52 142.5 53 154.0 54 201.5 55 309.0 56 321.0 57 207.0 58 169.0 59 183.5 60 132.0 61 76.0 62 50.5 63 39.0 64 24.5 65 10.0 66 6.0 67 3.0 68 5.0 69 3.0 70 3.0 71 5.0 72 7.5 73 7.0 74 2.5 75 1.5 76 1.5 77 0.5 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1 2 0.27499999999999997 3 0.0 4 0.0 5 0.6 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.2375 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.45 #Duplication Level Percentage of deduplicated Percentage of total 1 77.11719418306244 45.074999999999996 2 10.906757912745936 12.75 3 5.175363558597092 9.075 4 2.1813515825491874 5.1 5 1.2403763900769889 3.6249999999999996 6 0.8126603934987169 2.85 7 0.2566295979469632 1.05 8 0.42771599657827203 2.0 9 0.1283147989734816 0.675 >10 1.7108639863130881 16.35 >50 0.0427715996578272 1.4500000000000002 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GGCATGATGACTTGGCCTCATCCTCTCCTTCCTCCGGCTTAACACCGGCG 58 1.4500000000000002 No Hit CCCCACTGCTGCCTCCCGTAGGAGTCTGGGCCGTGTCTCAGTCCCAGTGT 48 1.2 No Hit GTGCTCTGCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTCCTA 47 1.175 No Hit GGCCAAAATAACCATGAGCAGCCACAATATTATAAGTCTCTTCCTCTTGA 34 0.8500000000000001 No Hit GCGGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTACG 33 0.8250000000000001 No Hit GTCCAGTCAACTGCTGCGCCTCAACGCATTTCGGGGAGAACCAGCTAGCT 25 0.625 No Hit GCTCTGCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTCCTAAA 25 0.625 No Hit CTCTGCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTCCTAAAG 21 0.525 No Hit CCGGGAACGGATTCACCGCCGTATGGCTGACCGGCGATTACTAGCGATTC 19 0.475 No Hit GGGGCATGATGACTTGGCCTCATCCTCTCCTTCCTCCGGCTTAACACCGG 18 0.44999999999999996 No Hit GCCACCTACAGACGCTTTACGCCCAATCATTCCGGATAACGCTTGCATCC 18 0.44999999999999996 No Hit GCTTTCTTTTCCTCTGGCTACTAAGATGTTTCAGTTCGCCAGGTTGTCTC 18 0.44999999999999996 No Hit CCCGTCAATTCCTTTGAGTTTCATTCTTGCGAACGTACTCCCCAGGCGGG 17 0.42500000000000004 No Hit GTCCCTATTAATCATTACTCCGATCCCGAAGGCCAACACAATAGGACCGG 16 0.4 No Hit GTCCCAGTGTGGCTGATCATCCTCTCGGACCAGCTACTGATCATCGCCTT 15 0.375 No Hit GCCAAAATAACCATGAGCAGCCACAATATTATAAGTCTCTTCCTCTTGAC 15 0.375 No Hit GGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTACGTT 15 0.375 No Hit GTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTC 13 0.325 No Hit CCTCAGCCTACGGGGTATTAGCAACCGTTTCCAGTTGTTGTTCCCCTCCC 13 0.325 No Hit GTGAGCATTACGTTCGTGCATTACTTCCATACCAAGATTAGCACGGTTGA 13 0.325 No Hit GCCCAATCATTCCGGATAACGCTTGCATCCTCTGTCTTACCGCGGCTGCT 13 0.325 No Hit CCTAGCTTTCGTCTCTCAGTGTCAGTGTCGGCCCAGCAGAGTGCTTTCGC 13 0.325 No Hit GCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTCCTAAAGGCAT 13 0.325 No Hit GTCCTTAAACCTATAACCATCTTTCGGCTAACCTAGCCTCCTCCGTCCCT 13 0.325 No Hit CTCTGCCCCTACCGTACTCCAGCTTGGTAGTTTCCACCGCCTGTCCAGGG 13 0.325 No Hit GGATGCATAAGGATGTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAA 12 0.3 No Hit GTCTCTCAGTGTCAGTGTCGGCCCAGCAGAGTGCTTTCGCCGTTGGTGTT 11 0.27499999999999997 No Hit GCTGCGATGAAGGCGATAATAAATACAGAAGTTGCGGTCAATAAGGTAGG 11 0.27499999999999997 No Hit CCCACTGCTGCCTCCCGTAGGAGTCTGGGCCGTGTCTCAGTCCCAGTGTG 11 0.27499999999999997 No Hit GCTCCTCAGCCTACGGGGTATTAGCAACCGTTTCCAGTTGTTGTTCCCCT 11 0.27499999999999997 No Hit CCACTGCTGCCTCCCGTAGGAGTCTGGGCCGTGTCTCAGTCCCAGTGTGG 10 0.25 No Hit CCGTCAATTCCTTTGAGTTTCATTCTTGCGAACGTACTCCCCAGGCGGGA 10 0.25 No Hit GTTCTATTTCACTACCCACTGGGGGTTCTTTTCACCTTTCCCTCACGGTA 10 0.25 No Hit GTCGGTTTCGGGTACAGGTACCCTTTTGTTGAAGGTCGTTCGAGCTTTTC 10 0.25 No Hit GTCAATTCCTTTGAGTTTCATTCTTGCGAACGTACTCCCCAGGCGGGATA 10 0.25 No Hit GTTCGAGCTTTTCCTGGGAGTATGGCATCGGTTACATACTTCAGTGCCGT 10 0.25 No Hit CCCTACCGTACTCCAGCTTGGTAGTTTCCACCGCCTGTCCAGGGTTGAGC 10 0.25 No Hit CTAGCTTTCGTCTCTCAGTGTCAGTGTCGGCCCAGCAGAGTGCTTTCGCC 10 0.25 No Hit CCCATCACGATGAAATTTCCCAAGATTACCCGGGCCTGTCGGCCAAGGCT 10 0.25 No Hit GCACGTGTGTCGCCCAGGGCATAAGGGGCATGATGACTTGGCCTCATCCT 10 0.25 No Hit GCGGAAAAACCTTATCCATTAATAGCGGGAACTTCAAGAGCAGCTAGATC 10 0.25 No Hit GGAAAAACCTTATCCATTAATAGCGGGAACTTCAAGAGCAGCTAGATCTA 9 0.22499999999999998 No Hit GTTAGCGGAAAAACCTTATCCATTAATAGCGGGAACTTCAAGAGCAGCTA 9 0.22499999999999998 No Hit GGTCGTTCGAGCTTTTCCTGGGAGTATGGCATCGGTTACATACTTCAGTG 9 0.22499999999999998 No Hit GCCGAAAACACCAGCTACACCTAACATGTGAAATGGATGCATAAGGATGT 8 0.2 No Hit GGCGATAATAAATACAGAAGTTGCGGTCAATAAGGTAGGGATCATCAAAA 8 0.2 No Hit CCTCACGGTACTACTTCGCTATCGGTCACCCAGGAGTATTTAGCCTTGCA 8 0.2 No Hit CCATCGTTTACGGCTAGGACTACTGGGGTCTCTAATCCCATTTGCTCCCC 8 0.2 No Hit GTCGGCATCGTTTATGGTTGAGACTAGGACGGTATCTGATCGTCTTCGAG 8 0.2 No Hit GCCCCCGTCAATTCCTTTGAGTTTCATTCTTGCGAACGTACTCCCCAGGC 8 0.2 No Hit CCACAATATTATAAGTCTCTTCCTCTTGACCAAATTTGTAACCCTCATTA 8 0.2 No Hit GGGTGGGCTTACTACTTATATGCTTTCAGCAGTTATCCTCTCCGCACTTG 8 0.2 No Hit CTCCACGCGGCATTGCTCCGTCAGGCTTTCGCCCATTGCGGAAAATTCCC 8 0.2 No Hit CCTGTGTCGGTTTCGGGTACAGGTACCCTTTTGTTGAAGGTCGTTCGAGC 8 0.2 No Hit GTCATTGTTTCTTCTCCGAGAAAAGAAGTTGACGACCCGTAGGCCTTCCA 7 0.17500000000000002 No Hit GTACAAAGGGCAGGGACGTAGTCAACGCGAGCTGATGACTCGCGCTTACT 7 0.17500000000000002 No Hit GCATTACGTTCGTGCATTACTTCCATACCAAGATTAGCACGGTTGATGAT 7 0.17500000000000002 No Hit CCCTCATTAGCAGATTCATTTTCAGTAGTTTCCCTGATCAAACTAGAGGT 7 0.17500000000000002 No Hit CTCCTTTTGCTCCTCAGCCTACGGGGTATTAGCAACCGTTTCCAGTTGTT 7 0.17500000000000002 No Hit GTTGAAACTAGCATATTGGAAGATTAATCGGCCAAAATAACCATGAGCAG 7 0.17500000000000002 No Hit CACCGCTCCACCGGAAATTCCCTCTGCCCCTACCGTACTCCAGCTTGGTA 6 0.15 No Hit GACCTATTTGGGAATCTCCGGATCTATGCTTATTTTCAACTCCCCGAAGC 6 0.15 No Hit CCCCAGTTAAGTAGTCATGCATTACAATAGGAACACCTAATTCTCTCGCA 6 0.15 No Hit CTCCCATTTCGCTCGCCGCTACTACGGGAATCGCTTTTGCTTTCTTTTCC 6 0.15 No Hit GCGACCTTGACTATCAACTACAGATTGGTTGAAATTGAATCCATTTAGGT 6 0.15 No Hit GCATGATGACTTGGCCTCATCCTCTCCTTCCTCCGGCTTAACACCGGCGG 6 0.15 No Hit GCTAGTTATCCAGTTGCAGAAGCGACCCCACAGGCTTGTACTTTCGCGTC 6 0.15 No Hit GACCTGTTGTCCATCGACTACGCCTTTCGGCCTGATCTTAGGCCCTGACT 6 0.15 No Hit ACGCGACCTTGACTATCAACTACAGATTGGTTGAAATTGAATCCATTTAG 6 0.15 No Hit CACCTCCACGCGGCATTGCTCCGTCAGGCTTTCGCCCATTGCGGAAAATT 6 0.15 No Hit GTTCAGGGTTCCAAACTCATAGTGGCAACTAAACACGAGGGTTGCGCTCG 6 0.15 No Hit GCTCCCCTAGCTTTCGTCTCTCAGTGTCAGTGTCGGCCCAGCAGAGTGCT 6 0.15 No Hit CCTCCACGCGGCATTGCTCCGTCAGGCTTTCGCCCATTGCGGAAAATTCC 6 0.15 No Hit GCTTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCA 6 0.15 No Hit CTCTTGACCAAATTTGTAACCCTCATTAGCAGATTCATTTTCAGTAGTTT 6 0.15 No Hit GACCCATATAACAAGCTACACCAAGTAAGAAGTGTAGAACAATTAGCTCA 6 0.15 No Hit CTTTCTTTTCCTCTGGCTACTAAGATGTTTCAGTTCGCCAGGTTGTCTCT 6 0.15 No Hit CTCGCGAATACCATCAATATCTACTGGAGGGGCTGCGATGAAGGCGATAA 6 0.15 No Hit GGGGCTGCGATGAAGGCGATAATAAATACAGAAGTTGCGGTCAATAAGGT 6 0.15 No Hit GGGGGCATGATGACTTGGCCTCATCCTCTCCTTCCTCCGGCTTAACACCG 5 0.125 No Hit GCGGTCAATAAGGTAGGGATCATCAAAACACCGAACCATCCGATGTAAAG 5 0.125 No Hit CGTCAATTCCTTTGAGTTTCATTCTTGCGAACGTACTCCCCAGGCGGGAT 5 0.125 No Hit GGCGCATACCCAAACGGAAACTAAGTTCCCACTCACGACCCATATAACAA 5 0.125 No Hit GTACCGCATTAATGGGCGAACAGCCCAACCCTTGGAACCACCTACAGCTC 5 0.125 No Hit TCTGCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTCCTAAAGG 5 0.125 No Hit GTTGCGGTCAATAAGGTAGGGATCATCAAAACACCGAACCATCCGATGTA 5 0.125 No Hit CCTTAAACCTATAACCATCTTTCGGCTAACCTAGCCTCCTCCGTCCCTCC 5 0.125 No Hit CCTAGAGTAACTTTTATCCGTTGAGCGACGGCCCTTCCACTCGGCACCGT 5 0.125 No Hit GGGCATGATGACTTGGCCTCATCCTCTCCTTCCTCCGGCTTAACACCGGC 5 0.125 No Hit CTCCAGCTTGGTAGTTTCCACCGCCTGTCCAGGGTTGAGCCCTGGGATTT 5 0.125 No Hit CCACAGCTTCGGCAGATCGCTTAGCCCCGTTCATCTTCAGCGCAAGGGCG 5 0.125 No Hit CGGGAACGGATTCACCGCCGTATGGCTGACCGGCGATTACTAGCGATTCC 5 0.125 No Hit CAATCATTCCGGATAACGCTTGCATCCTCTGTCTTACCGCGGCTGCTGGC 5 0.125 No Hit GTAGGGATCATCAAAACACCGAACCATCCGATGTAAAGACGATTTTCAGT 5 0.125 No Hit GTTCGTTAACGGAATTAACCAGACAAATCGCTCCACCAACTAAGAACGGC 5 0.125 No Hit GCCCCTACCGTACTCCAGCTTGGTAGTTTCCACCGCCTGTCCAGGGTTGA 5 0.125 No Hit CCAAGTATTAATAACGCGACCTTGACTATCAACTACAGATTGGTTGAAAT 5 0.125 No Hit GTACAAGGCCCGGGAACGGATTCACCGCCGTATGGCTGACCGGCGATTAC 5 0.125 No Hit CGGGCTGTTTCCCTCTCGACGATGAAGCTTATCCCCCATCGTCTCACTGG 5 0.125 No Hit GGCCAAGCAGCCAAGAAGAAGTGTAAAGAACGAGAGTTGTTGAAACTAGC 5 0.125 No Hit CATCGTTTACGGCTAGGACTACTGGGGTCTCTAATCCCATTTGCTCCCCT 5 0.125 No Hit CTCAGATACCGTCATTGTTTCTTCTCCGAGAAAAGAAGTTGACGACCCGT 5 0.125 No Hit CTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCTA 5 0.125 No Hit GCCTCACCAACTAGCTAATCAGACGCGAGCCCCTCCTTGGGCGGATTTCT 5 0.125 No Hit GCCCATTGTAGCACGTGTGTCGCCCAGGGCATAAGGGGCATGATGACTTG 5 0.125 No Hit CCCTAGAGTAACTTTTATCCGTTGAGCGACGGCCCTTCCACTCGGCACCG 5 0.125 No Hit CGCGACCTTGACTATCAACTACAGATTGGTTGAAATTGAATCCATTTAGG 5 0.125 No Hit GTCTGTTCAGGGTTCCAAACTCATAGTGGCAACTAAACACGAGGGTTGCG 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0125 0.0 0.0 0.0 0.0 56-57 0.025 0.0 0.0 0.0 0.0 58-59 0.05 0.0 0.0 0.0 0.0 60-61 0.05 0.0 0.0 0.0 0.0 62-63 0.0625 0.0 0.0 0.0 0.0 64-65 0.1125 0.0 0.0 0.0 0.0 66-67 0.16249999999999998 0.0 0.0 0.0 0.0 68-69 0.225 0.0 0.0 0.0 0.0 70-71 0.35 0.0 0.0 0.0 0.0 72-73 0.475 0.0 0.0 0.0 0.0 74-75 0.5875 0.0 0.0 0.0 0.0 76-77 0.7375 0.0 0.0 0.0 0.0 78-79 0.8375 0.0 0.0 0.0 0.0 80-81 1.1375 0.0 0.0 0.0 0.0 82-83 1.3624999999999998 0.0 0.0 0.0 0.0 84-85 1.625 0.0 0.0 0.0 0.0 86-87 1.8625 0.0 0.0 0.0 0.0 88-89 2.1125 0.0 0.0 0.0 0.0 90-91 2.4875 0.0 0.0 0.0 0.0 92-93 2.925 0.0 0.0 0.0 0.0 94-95 3.4749999999999996 0.0 0.0 0.0 0.0 96-97 3.8625 0.0 0.0 0.0 0.0 98-99 4.25 0.0 0.0 0.0 0.0 100-101 4.6 0.0 0.0 0.0 0.0 102-103 5.075 0.0 0.0 0.0 0.0 104-105 5.637499999999999 0.0 0.0 0.0 0.0 106-107 6.275 0.0 0.0 0.0 0.0 108-109 6.7 0.0 0.0 0.0 0.0 110-111 7.2625 0.0 0.0 0.0 0.0 112-113 7.9375 0.0 0.0 0.0 0.0 114-115 8.45 0.0 0.0 0.0 0.0 116-117 9.149999999999999 0.0 0.0 0.0 0.0 118-119 9.825 0.0 0.0 0.0 0.0 120-121 10.462499999999999 0.0 0.0 0.0 0.0 122-123 10.9875 0.0 0.0 0.0 0.0 124-125 11.6875 0.0 0.0 0.0 0.0 126-127 12.45 0.0 0.0 0.0 0.0 128-129 12.9875 0.0 0.0 0.0 0.0 130-131 13.712499999999999 0.0 0.0 0.0 0.0 132-133 14.4375 0.0 0.0 0.0 0.0 134-135 14.875 0.0 0.0 0.0 0.0 136-137 15.5 0.0 0.0 0.0 0.0 138-139 16.3125 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGCCAAA 10 0.0065840036 146.77216 1 GCCAAAA 10 0.0065840036 146.77216 2 ACGGTAC 10 0.0065840036 146.77216 5 CGGTACT 10 0.0068396386 144.9375 6 ATAACCA 30 0.0018004265 72.46875 8 TAACCAT 30 0.0018004265 72.46875 9 >>END_MODULE SRR6941573 read2 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR6941573_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 49 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 33.996 35.0 35.0 35.0 32.0 35.0 2 34.24175 35.0 35.0 35.0 33.0 35.0 3 34.20175 35.0 35.0 35.0 32.0 35.0 4 34.09725 35.0 35.0 35.0 32.0 35.0 5 34.27275 35.0 35.0 35.0 33.0 35.0 6 38.83175 40.0 39.0 40.0 37.0 40.0 7 38.7655 40.0 39.0 40.0 37.0 40.0 8 38.8285 40.0 39.0 40.0 37.0 40.0 9 38.898 40.0 40.0 40.0 37.0 40.0 10-14 38.814699999999995 40.0 39.8 40.0 37.0 40.0 15-19 38.84685 40.0 39.8 40.0 37.4 40.0 20-24 38.85210000000001 40.0 40.0 40.0 37.0 40.0 25-29 38.84295 40.0 39.8 40.0 37.0 40.0 30-34 38.717150000000004 40.0 39.6 40.0 36.6 40.0 35-39 38.79545 40.0 39.0 40.0 37.0 40.0 40-44 38.61435 40.0 39.2 40.0 36.4 40.0 45-49 38.4641 40.0 39.0 40.0 36.0 40.0 50-54 38.44795 40.0 39.0 40.0 36.0 40.0 55-59 38.54585 40.0 39.0 40.0 36.0 40.0 60-64 38.51875 40.0 39.0 40.0 36.0 40.0 65-69 38.344849999999994 40.0 39.0 40.0 35.6 40.0 70-74 38.2767 40.0 39.0 40.0 35.4 40.0 75-79 38.12335 40.0 39.0 40.0 35.0 40.0 80-84 38.1541 40.0 39.0 40.0 35.0 40.0 85-89 38.094100000000005 40.0 39.0 40.0 34.4 40.0 90-94 38.0239 40.0 39.0 40.0 34.4 40.0 95-99 37.7821 40.0 39.0 40.0 34.0 40.0 100-104 36.86825 39.0 37.6 39.6 32.0 39.8 105-109 37.39450000000001 40.0 39.0 40.0 34.0 40.0 110-114 33.872699999999995 36.4 34.2 38.2 26.6 38.6 115-119 17.9546 16.6 15.4 23.2 6.8 30.0 120-124 2.0 2.0 2.0 2.0 2.0 2.0 125-129 2.0 2.0 2.0 2.0 2.0 2.0 130-134 2.0 2.0 2.0 2.0 2.0 2.0 135-139 2.0 2.0 2.0 2.0 2.0 2.0 140-144 2.0 2.0 2.0 2.0 2.0 2.0 145-149 2.0 2.0 2.0 2.0 2.0 2.0 150-151 2.0 2.0 2.0 2.0 2.0 2.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 0.0 4 2.0 5 0.0 6 2.0 7 1.0 8 0.0 9 0.0 10 2.0 11 0.0 12 2.0 13 1.0 14 3.0 15 6.0 16 6.0 17 5.0 18 21.0 19 10.0 20 20.0 21 30.0 22 53.0 23 46.0 24 62.0 25 63.0 26 93.0 27 128.0 28 189.0 29 349.0 30 1931.0 31 972.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 53.128128128128125 16.49149149149149 6.806806806806807 23.573573573573572 2 28.982245561390346 21.630407601900476 27.656914228557138 21.73043260815204 3 23.43085771442861 22.255563890972745 33.50837709427357 20.80520130032508 4 27.28182045511378 29.582395598899723 22.95573893473368 20.180045011252815 5 31.58289572393098 31.45786446611653 19.10477619404851 17.854463615903978 6 24.125 36.85 19.400000000000002 19.625 7 21.224999999999998 21.224999999999998 36.025 21.525 8 23.799999999999997 24.075 25.624999999999996 26.5 9 26.424999999999997 21.025 27.950000000000003 24.6 10-14 27.650000000000002 25.515 24.834999999999997 22.0 15-19 28.37 25.295 24.525 21.81 20-24 27.655 24.975 25.34 22.03 25-29 28.185 25.615 23.794999999999998 22.405 30-34 28.110000000000003 26.32 23.935000000000002 21.634999999999998 35-39 27.529999999999998 26.055 25.34 21.075 40-44 27.250000000000004 26.845000000000002 24.19 21.715 45-49 27.57757757757758 27.102102102102105 24.0990990990991 21.22122122122122 50-54 28.655731146229247 24.8249649929986 24.93498699739948 21.584316863372674 55-59 27.336834208552137 26.4766191547887 24.511127781945486 21.67541885471368 60-64 27.114067110066507 25.603840576086412 25.068760314047108 22.21333199979997 65-69 28.622862286228624 25.587558755875587 24.282428242824285 21.507150715071507 70-74 28.049207381107166 25.588838325748863 25.068760314047108 21.293193979096863 75-79 28.74 25.095 24.21 21.955 80-84 27.70554110822164 25.370074014802963 26.050210042008402 20.874174834966993 85-89 29.080000000000002 25.06 24.04 21.82 90-94 27.994999999999997 25.735000000000003 24.315 21.955 95-99 28.29 25.03 24.845 21.834999999999997 100-104 27.889999999999997 27.224999999999998 24.154999999999998 20.73 105-109 28.325 24.15 25.335 22.189999999999998 110-114 28.432551613549812 26.14251352976548 24.61916215674484 20.80577269993987 115-119 28.61002890536634 25.769762473293955 24.50672363956265 21.11348498177705 120-124 26.408473170420464 41.13443501563198 15.497990174184903 16.95910163976265 125-129 NaN NaN NaN NaN 130-134 28.921568627450984 26.34489693313223 22.373051784816493 22.360482654600304 135-139 28.333249635916236 25.746999447597048 24.421232360769345 21.49851855571737 140-144 29.013710747456877 26.20837808807734 24.65407215517786 20.123839009287924 145-149 28.93223305826457 24.8062015503876 24.006001500375092 22.255563890972745 150-151 28.32811521603006 26.086412022542266 23.118346900438322 22.467125860989352 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.5 23 1.0 24 2.0 25 4.5 26 8.0 27 7.5 28 7.5 29 12.5 30 16.0 31 15.0 32 17.5 33 21.5 34 30.5 35 40.0 36 51.0 37 75.5 38 90.0 39 101.0 40 119.5 41 126.5 42 112.0 43 109.5 44 123.0 45 113.0 46 112.5 47 115.5 48 104.0 49 104.0 50 116.0 51 142.0 52 137.0 53 157.5 54 287.0 55 345.5 56 267.0 57 185.5 58 145.5 59 138.0 60 122.0 61 83.5 62 64.5 63 47.0 64 23.5 65 16.5 66 11.5 67 11.0 68 11.5 69 11.5 70 10.0 71 9.0 72 5.0 73 1.0 74 1.0 75 2.5 76 2.0 77 1.0 78 2.5 79 1.5 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 0.1 2 0.025 3 0.025 4 0.025 5 0.025 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.1 50-54 0.02 55-59 0.025 60-64 0.015 65-69 0.01 70-74 0.015 75-79 0.0 80-84 0.02 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.22 115-119 20.43 120-124 21.634999999999998 125-129 100.0 130-134 60.22 135-139 0.43499999999999994 140-144 20.865000000000002 145-149 80.00500000000001 150-151 0.1875 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 65.375 #Duplication Level Percentage of deduplicated Percentage of total 1 77.36137667304016 50.575 2 12.08413001912046 15.8 3 5.009560229445507 9.825000000000001 4 2.5239005736137665 6.6000000000000005 5 0.8413001912045889 2.75 6 0.6883365200764818 2.7 7 0.30592734225621415 1.4000000000000001 8 0.30592734225621415 1.6 9 0.19120458891013384 1.125 >10 0.6500956022944551 6.1 >50 0.03824091778202677 1.525 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTTCGATCCTGGCTCAGGATGAACGCTGGCGGCATGCTTAACACATGCAA 61 1.525 No Hit CCTTACCAGGGCTTGACATGCCGCGAATCCTCTTGAAAGAGAGGGGTGCC 33 0.8250000000000001 No Hit GCTTGTTATATGGGTCGTGAGTGGGAACTTAGTTTCCGTTTGGGTATGCG 22 0.5499999999999999 No Hit GGCTTGCGGTGGATACCTAGGCACCCAGAGACGAGGAAGGGCGTAGCAAG 20 0.5 No Hit GCCTGACGGAGCAATGCCGCGTGGAGGTGGAAGGCCTACGGGTCGTCAAC 17 0.42500000000000004 No Hit GCGAAAGCCTGACGGAGCAATGCCGCGTGGAGGTGGAAGGCCTACGGGTC 13 0.325 No Hit CAGTGACCAGGCCCGGGCGACTGTTTACCAAAAACACAGGTCTCCGCAAA 13 0.325 No Hit GCACAACATCCTTATGCATCCATTTCACATGTTAGGTGTAGCTGGTGTTT 13 0.325 No Hit TATCTATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTT 13 0.325 No Hit AGAACCTTACCAGGGCTTGACATGCCGCGAATCCTCTTGAAAGAGAGGGG 13 0.325 No Hit GCTTGCGGTGGATACCTAGGCACCCAGAGACGAGGAAGGGCGTAGCAAGC 12 0.3 No Hit GTGGGAACTTAGTTTCCGTTTGGGTATGCGCCCTTGGATTGCTGTTGCAT 12 0.3 No Hit GAAACAATGACGGTATCTGAGGAATAAGCATCGGCTAACTCTGTGCCAGC 12 0.3 No Hit GTTGCATATTCAGCTCCTGTTGCAGCTGCGACTGCTGTTTTCTTGATTTA 11 0.27499999999999997 No Hit GAACCTTACCAGGGCTTGACATGCCGCGAATCCTCTTGAAAGAGAGGGGT 10 0.25 No Hit CCTGCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGCAA 10 0.25 No Hit GTTATTTTGGCCGATTAATCTTCCAATATGCTAGTTTCAACAACTCTCGT 10 0.25 No Hit GTTATACAATGGTGGTCCTTATGAGCTAATTGTTCTACACTTCTTACTTG 10 0.25 No Hit CAGGGCTTGACATGCCGCGAATCCTCTTGAAAGAGAGGGGTGCCCTCGGG 9 0.22499999999999998 No Hit CAACAACTGGAAACGGTTGCTAATACCCCGTAGGCTGAGGAGCAAAAGGA 9 0.22499999999999998 No Hit GATGAACGCTGGCGGCATGCTTAACACATGCAAGTCGAACGGGAAGTGGT 9 0.22499999999999998 No Hit GTTGGGTTAAGTCTCGCAACGAGCGCAACCCTCGTGTTTAGTTGCCACTA 9 0.22499999999999998 No Hit GTTAGGTGTAGCTGGTGTTTTCGGCGGTTCCCTATTCAGTGCTATGCATG 9 0.22499999999999998 No Hit AGCACAACATCCTTATGCATCCATTTCACATGTTAGGTGTAGCTGGTGTT 8 0.2 No Hit GGATGAACGCTGGCGGCATGCTTAACACATGCAAGTCGAACGGGAAGTGG 8 0.2 No Hit CCTGGCTCAGGATGAACGCTGGCGGCATGCTTAACACATGCAAGTCGAAC 8 0.2 No Hit GGGAATTTCCGGTGGAGCGGTGAAATGCATTGAGATCGGAAAGAACACCA 8 0.2 No Hit GCTAGTTTCAACAACTCTCGTTCTTTACACTTCTTCTTGGCTGCTTGGCC 8 0.2 No Hit ATTATCTATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAAT 8 0.2 No Hit GTTCAACCTAAATGGATTCAATTTCAACCAATCTGTAGTTGATAGTCAAG 8 0.2 No Hit GTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGATCTTACCATGAC 8 0.2 No Hit GTCGTGAGTGGGAACTTAGTTTCCGTTTGGGTATGCGCCCTTGGATTGCT 7 0.17500000000000002 No Hit GTTAAGTCTCGCAACGAGCGCAACCCTCGTGTTTAGTTGCCACTATGAGT 7 0.17500000000000002 No Hit GGAAGGCCTACGGGTCGTCAACTTCTTTTCTCGGAGAAGAAACAATGACG 7 0.17500000000000002 No Hit CTATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAA 7 0.17500000000000002 No Hit AGTGACCAGGCCCGGGCGACTGTTTACCAAAAACACAGGTCTCCGCAAAG 7 0.17500000000000002 No Hit CCTAGGCACCCAGAGACGAGGAAGGGCGTAGCAAGCGACGAAATGCTTCG 7 0.17500000000000002 No Hit GGCAGAGGGAATTTCCGGTGGAGCGGTGAAATGCATTGAGATCGGAAAGA 7 0.17500000000000002 No Hit GTTTGATCAGGGAAACTACTGAAAATGAATCTGCTAATGAGGGTTACAAA 7 0.17500000000000002 No Hit GTATGCGCCCTTGGATTGCTGTTGCATATTCAGCTCCTGTTGCAGCTGCG 6 0.15 No Hit GCTAATTGTTCTACACTTCTTACTTGGTGTAGCTTGTTATATGGGTCGTG 6 0.15 No Hit CTGCATCCGTTGATGAATGGTTATACAATGGTGGTCCTTATGAGCTAATT 6 0.15 No Hit GGGAAACTACTGAAAATGAATCTGCTAATGAGGGTTACAAATTTGGTCAA 6 0.15 No Hit GGGAAGCAACCGCGAAAGCGGGGGTCGACGAAGCGGAAGCGAGAATGTCG 6 0.15 No Hit GGAGGGGCTCGCGTCTGATTAGCTAGTTGGTGAGGCAATAGCTTACCAAG 6 0.15 No Hit GGGAGCTTGACTGCAAGACTCACCCGTCGAGCAGAGACGAAAGTCGGCCT 6 0.15 No Hit CTGAGGAATAAGCATCGGCTAACTCTGTGCCAGCAGCCGCGGTAAGACAG 6 0.15 No Hit GCCGCGAATCCTCTTGAAAGAGAGGGGTGCCCTCGGGAACGCGGACACAG 6 0.15 No Hit GAGAAATCCGCCCAAGGAGGGGCTCGCGTCTGATTAGCTAGTTGGTGAGG 6 0.15 No Hit GCATCGGCTAACTCTGTGCCAGCAGCCGCGGTAAGACAGAGGATGCAAGC 6 0.15 No Hit CTTAGTTTCCGTTTGGGTATGCGCCCTTGGATTGCTGTTGCATATTCAGC 6 0.15 No Hit GGAAGCTTCTCTGATGGTATGCCTTTAGGAATATCTGGTACTTTCAACTT 6 0.15 No Hit GGTTGCTAATACCCCGTAGGCTGAGGAGCAAAAGGAGAAATCCGCCCAAG 6 0.15 No Hit GGCGATGATCAGTAGCTGGTCCGAGAGGATGATCAGCCACACTGGGACTG 6 0.15 No Hit GGTAGGGGCAGAGGGAATTTCCGGTGGAGCGGTGAAATGCATTGAGATCG 6 0.15 No Hit TGATGGTATTCGCGAGCCTGTTTCTGGTTCTTTACTTTATGGAAACAATA 6 0.15 No Hit CAGGATGAACGCTGGCGGCATGCTTAACACATGCAAGTCGAACGGGAAGT 6 0.15 No Hit GGCTGTCGTCAGCTCGTGCCGTAAGGTGTTGGGTTAAGTCTCGCAACGAG 5 0.125 No Hit GATCAGTAGCTGGTCCGAGAGGATGATCAGCCACACTGGGACTGAGACAC 5 0.125 No Hit GCACTGTTTCGGTGCGGGCTGCGCGAGCGGTACCAAATCGAGGCAAACTC 5 0.125 No Hit GGAAAGAACACCAACGGCGAAAGCACTCTGCTGGGCCGACACTGACACTG 5 0.125 No Hit GCATGGCTGTCGTCAGCTCGTGCCGTAAGGTGTTGGGTTAAGTCTCGCAA 5 0.125 No Hit AGCGTCTGTAGGTGGCTTTTCAAGTCCGCCGTCAAATCCCAGGGCTCAAC 5 0.125 No Hit GAGACTTATAATATTGTGGCTGCTCATGGTTATTTTGGCCGATTAATCTT 5 0.125 No Hit GTTGAAAATAAGCATAGATCCGGAGATTCCCAAATAGGTCAACCTTTTAA 5 0.125 No Hit CATGCCGCGAATCCTCTTGAAAGAGAGGGGTGCCCTCGGGAACGCGGACA 5 0.125 No Hit GCTGTTTTCTTGATTTACCCTATTGGTCAAGGAAGCTTCTCTGATGGTAT 5 0.125 No Hit GCATGGTTCCTTGGTAACCTCTAGTTTGATCAGGGAAACTACTGAAAATG 5 0.125 No Hit CAGGTCAATATTCCTGTACTACCCCTTGTTGGTACGGAGGGACGGAGGAG 5 0.125 No Hit CGGACATTGGTCCTCGAGTGCAAAGGCAGAAGGGAGCTTGACTGCAAGAC 5 0.125 No Hit AGGGAATTTCCGGTGGAGCGGTGAAATGCATTGAGATCGGAAAGAACACC 5 0.125 No Hit AGTCAAGGTCGCGTTATTAATACTTGGGCTGATATCATCAACCGTGCTAA 5 0.125 No Hit GTCGGCTTGAGTAACGAAAACATTGGTGAGAATCCAATGCCCCGAAAACC 5 0.125 No Hit ACCTTACCAGGGCTTGACATGCCGCGAATCCTCTTGAAAGAGAGGGGTGC 5 0.125 No Hit CCTACGGGTCGTCAACTTCTTTTCTCGGAGAAGAAACAATGACGGTATCT 5 0.125 No Hit CGGGTGAGTAACGCGTAAGAACCTGCCCTTGGGAGGGGAACAACAACTGG 5 0.125 No Hit GTGAAATAGAACGTGAAACCGTGCTGAGCTCCCAAGCAGTGGGAGGGGAA 5 0.125 No Hit CTTCCAATATGCTAGTTTCAACAACTCTCGTTCTTTACACTTCTTCTTGG 5 0.125 No Hit GCTTAACACATGCAAGTCGAACGGGAAGTGGTGTTTCCAGTGGCGAACGG 5 0.125 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0125 0.0 0.0 0.0 0.0 56-57 0.025 0.0 0.0 0.0 0.0 58-59 0.05 0.0 0.0 0.0 0.0 60-61 0.05 0.0 0.0 0.0 0.0 62-63 0.0625 0.0 0.0 0.0 0.0 64-65 0.1375 0.0 0.0 0.0 0.0 66-67 0.1875 0.0 0.0 0.0 0.0 68-69 0.25 0.0 0.0 0.0 0.0 70-71 0.375 0.0 0.0 0.0 0.0 72-73 0.5 0.0 0.0 0.0 0.0 74-75 0.6125 0.0 0.0 0.0 0.0 76-77 0.7625 0.0 0.0 0.0 0.0 78-79 0.8625 0.0 0.0 0.0 0.0 80-81 1.1375 0.0 0.0 0.0 0.0 82-83 1.375 0.0 0.0 0.0 0.0 84-85 1.675 0.0 0.0 0.0 0.0 86-87 1.9125 0.0 0.0 0.0 0.0 88-89 2.1875 0.0 0.0 0.0 0.0 90-91 2.55 0.0 0.0 0.0 0.0 92-93 2.9375 0.0 0.0 0.0 0.0 94-95 3.4749999999999996 0.0 0.0 0.0 0.0 96-97 3.8875 0.0 0.0 0.0 0.0 98-99 4.275 0.0 0.0 0.0 0.0 100-101 4.625 0.0 0.0 0.0 0.0 102-103 5.1 0.0 0.0 0.0 0.0 104-105 5.6875 0.0 0.0 0.0 0.0 106-107 6.2875 0.0 0.0 0.0 0.0 108-109 6.375 0.0 0.0 0.0 0.0 110-111 6.375 0.0 0.0 0.0 0.0 112-113 6.375 0.0 0.0 0.0 0.0 114-115 6.375 0.0 0.0 0.0 0.0 116-117 6.375 0.0 0.0 0.0 0.0 118-119 6.375 0.0 0.0 0.0 0.0 120-121 6.375 0.0 0.0 0.0 0.0 122-123 6.375 0.0 0.0 0.0 0.0 124-125 6.375 0.0 0.0 0.0 0.0 126-127 6.375 0.0 0.0 0.0 0.0 128-129 6.375 0.0 0.0 0.0 0.0 130-131 6.375 0.0 0.0 0.0 0.0 132-133 6.375 0.0 0.0 0.0 0.0 134-135 6.375 0.0 0.0 0.0 0.0 136-137 6.375 0.0 0.0 0.0 0.0 138-139 6.375 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content pass >>END_MODULE Read 1840056 spots for SRR6941573.sra Written 1840056 spots for SRR6941573.sra Read 1840056 spots for SRR6941573.sra Written 1840056 spots for SRR6941573.sra Read 1840056 spots for SRR6941573.sra Written 1840056 spots for SRR6941573.sra Read 1840056 spots for SRR6941573.sra Written 1840056 spots for SRR6941573.sra Read 1840075 spots for SRR6941573.sra Written 1840075 spots for SRR6941573.sra Read 1840056 spots for SRR6941573.sra Written 1840056 spots for SRR6941573.sra Read 1840056 spots for SRR6941573.sra Written 1840056 spots for SRR6941573.sra Read 1840056 spots for SRR6941573.sra Written 1840056 spots for SRR6941573.sra Read 1840056 spots for SRR6941573.sra Written 1840056 spots for SRR6941573.sra Read 1840056 spots for SRR6941573.sra Written 1840056 spots for SRR6941573.sra Read 1840056 spots for SRR6941573.sra Written 1840056 spots for SRR6941573.sra Read 1840056 spots for SRR6941573.sra Written 1840056 spots for SRR6941573.sra Read 1840056 spots for SRR6941573.sra Written 1840056 spots for SRR6941573.sra Read 1840056 spots for SRR6941573.sra Written 1840056 spots for SRR6941573.sra Read 1840056 spots for SRR6941573.sra Written 1840056 spots for SRR6941573.sra Read 1840056 spots for SRR6941573.sra Written 1840056 spots for SRR6941573.sra Read 1840056 spots for SRR6941573.sra Written 1840056 spots for SRR6941573.sra Read 1840056 spots for SRR6941573.sra Written 1840056 spots for SRR6941573.sra Read 1840056 spots for SRR6941573.sra Written 1840056 spots for SRR6941573.sra Read 1840056 spots for SRR6941573.sra Written 1840056 spots for SRR6941573.sra SRR ids: ['SRR6941573.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_9a6tjsf7 SRR6941573.sra spots: 36801139 blocks: [[1, 1840056], [1840057, 3680112], [3680113, 5520168], [5520169, 7360224], [7360225, 9200280], [9200281, 11040336], [11040337, 12880392], [12880393, 14720448], [14720449, 16560504], [16560505, 18400560], [18400561, 20240616], [20240617, 22080672], [22080673, 23920728], [23920729, 25760784], [25760785, 27600840], [27600841, 29440896], [29440897, 31280952], [31280953, 33121008], [33121009, 34961064], [34961065, 36801139]] SRR6941573 file size 12448998 SRR6941573 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR6941573 SRR6941573_1.fastq SRR6941573_2.fastq Input file: SRR6941573_1.fastq Paired file: SRR6941573_2.fastq trimmed: SRR6941573-trimmed-pair1.fastq, SRR6941573-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Fri Dec 6 11:50:27 2024 >> started Fri Dec 6 11:51:20 2024 >> done (52.241s) 36801139 read pairs processed; of these: 44085 ( 0.12%) short read pairs filtered out after trimming by size control 41271 ( 0.11%) empty read pairs filtered out after trimming by size control 36715783 (99.77%) read pairs available; of these: 31120970 (84.76%) trimmed read pairs available after processing 5594813 (15.24%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 6 0.00% 19 6 0.00% 20 7 0.00% 21 11 0.00% 22 10 0.00% 23 11 0.00% 24 18 0.00% 25 13 0.00% 26 34 0.00% 27 38 0.00% 28 43 0.00% 29 64 0.00% 30 66 0.00% 31 86 0.00% 32 118 0.00% 33 148 0.00% 34 154 0.00% 35 167 0.00% 36 186 0.00% 37 221 0.00% 38 255 0.00% 39 320 0.00% 40 391 0.00% 41 455 0.00% 42 454 0.00% 43 516 0.00% 44 574 0.00% 45 631 0.00% 46 753 0.00% 47 778 0.00% 48 985 0.00% 49 1121 0.00% 50 1155 0.00% 51 1445 0.00% 52 1684 0.00% 53 1794 0.00% 54 2218 0.01% 55 2392 0.01% 56 2611 0.01% 57 2990 0.01% 58 3534 0.01% 59 3889 0.01% 60 4221 0.01% 61 5705 0.02% 62 6647 0.02% 63 6844 0.02% 64 7658 0.02% 65 8415 0.02% 66 8923 0.02% 67 9701 0.03% 68 11920 0.03% 69 14493 0.04% 70 15432 0.04% 71 16236 0.04% 72 20546 0.06% 73 22132 0.06% 74 21190 0.06% 75 24625 0.07% 76 26335 0.07% 77 31929 0.09% 78 29700 0.08% 79 34305 0.09% 80 36555 0.10% 81 38721 0.11% 82 42274 0.12% 83 45623 0.12% 84 48519 0.13% 85 58193 0.16% 86 61555 0.17% 87 64359 0.18% 88 73099 0.20% 89 68534 0.19% 90 72266 0.20% 91 76056 0.21% 92 81337 0.22% 93 89421 0.24% 94 97623 0.27% 95 89081 0.24% 96 86438 0.24% 97 94010 0.26% 98 88551 0.24% 99 93203 0.25% 100 94520 0.26% 101 101567 0.28% 102 107474 0.29% 103 110652 0.30% 104 117131 0.32% 105 112976 0.31% 106 113991 0.31% 107 116220 0.32% 108 120123 0.33% 109 146984 0.40% 110 125906 0.34% 111 140690 0.38% 112 170366 0.46% 113 123633 0.34% 114 150428 0.41% 115 139988 0.38% 116 177555 0.48% 117 179712 0.49% 118 174150 0.47% 119 201672 0.55% 120 218975 0.60% 121 215461 0.59% 122 218314 0.59% 123 239472 0.65% 124 244265 0.67% 125 258089 0.70% 126 256917 0.70% 127 276613 0.75% 128 319857 0.87% 129 444656 1.21% 130 773306 2.11% 131 1553854 4.23% 132 1092120 2.97% 133 5166805 14.07% 134 6437677 17.53% 135 133449 0.36% 136 110920 0.30% 137 113370 0.31% 138 131829 0.36% 139 188002 0.51% 140 826445 2.25% 141 2086930 5.68% 142 308594 0.84% 143 2450248 6.67% 144 163254 0.44% 145 735589 2.00% 146 70036 0.19% 147 84094 0.23% 148 107167 0.29% 149 168838 0.46% 150 1235359 3.36% 151 5594813 15.24% 36715783 reads passed initial QC criterion=sequence-density sequence-density=0.76 sequence-density-rank=1 fanout-score=5.76 fanout-score-rank=15 prefix-density=2.57 prefix-fanout=1.7 sequence=ATTTAGCCTTGGACGGAGTCTACCGCCCGATTTGGGCTGCATTCCCAAACAACCCGACTCGTTGACGGCGCCTCGTGGGGCGACAGGGTCCGGGCCGGACGGGGCTCTCACCCTCCCAGGCGCCCCTTTCCAGGGGACTTGGGCCCGGTCCGTCGCTGAGGACGCCTCTCCAGACTACAATTCGGACGGCACGGCCGCCCGATTCTCAAGCTGGGCTGCTCCCGGTTCGCTCGCCGTTACTAGGGGAATCCTTGTAAGTTTCTTCTCCTCCGCTTATTTATATGCTTAAACTCAGCGGGTAGTCCCGCCTGACCTGG criterion=fanout-score sequence-density=0.01 sequence-density-rank=36 fanout-score=47.30 fanout-score-rank=1 prefix-density=0.66 prefix-fanout=1.0 sequence=GCATCGCCGGCCCCCATCCGCTTCCCTCCCGGCAATTTCAAGCACTCTTTGACTCTCTTTTCAAAGTCCTTTTCATCTTTCCCTCGCGGTACTTGTTCGCTATCGG criterion=sequence-density sequence-density=0.53 sequence-density-rank=1 fanout-score=9.33 fanout-score-rank=6 prefix-density=3.76 prefix-fanout=1.3 sequence=TGGTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGTTAACGAACGAGACCTCAGCCTGCTAACTAGCTATGCGGAGCCATCCCTCCGCAGCTAGCTTCTTAGAGGGACTATCGCCGTTTAGGCGACGGAAGTTTGAGGCAATAACAGGTCTGTGATGCCCTTAGATGTTCTGGGCCGCACGCGCGCTACACTGATGTATTCAACGAGTATATAGCCTTGGCCGACAGGCCCGGGTAATCTTGGGAAATTTCATCGTGATGGGGATAGATCATTGCAATTGTTGGTCTTCAACGAGGAATGCCTAGTAAGCGCGAGTCATCAGCTCGCGTTGACTACGTCCCTGCCCTTTGTACACACC criterion=fanout-score sequence-density=0.01 sequence-density-rank=37 fanout-score=134.06 fanout-score-rank=1 prefix-density=1.32 prefix-fanout=1.1 sequence=AGAAGGGGTGCCCCCTCACAAAAGGGGGTCGCAGTGACCAGGCCCGGGCGACTGTTTACCAAAAACACAGGTCTCCGCAAAGTCGTAAGACCATGTATGGGGGCTGACGCCTGCCCAGTGCCGGAAGGTCAAGGAAGTTGGTGAACTGATGACAGGGAAGCCGGCGACCGAAGCCCCGGTGAACGGCGGCCGTAAC SRR6941573 testing PE reads STAR mapping to Ensembl genome Started job on | Dec 06 11:51:57 Started mapping on | Dec 06 11:51:57 Finished on | Dec 06 11:54:26 Mapping speed, Million of reads per hour | 887.09 Number of input reads | 36715783 Average input read length | 267 UNIQUE READS: Uniquely mapped reads number | 14863861 Uniquely mapped reads % | 40.48% Average mapped length | 271.89 Number of splices: Total | 2145275 Number of splices: Annotated (sjdb) | 1625245 Number of splices: GT/AG | 1740215 Number of splices: GC/AG | 26488 Number of splices: AT/AC | 9917 Number of splices: Non-canonical | 368655 Mismatch rate per base, % | 0.35% Deletion rate per base | 0.01% Deletion average length | 1.72 Insertion rate per base | 0.01% Insertion average length | 2.09 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 15017247 % of reads mapped to multiple loci | 40.90% Number of reads mapped to too many loci | 703231 % of reads mapped to too many loci | 1.92% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 7.11% % of reads unmapped: other | 9.59% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 6860348 6860348 6860348 N_multimapping 15017247 15017247 15017247 N_noFeature 9766582 14581302 9887394 N_ambiguous 385715 7493 226938 UnstrandedReadsAssigned:4711564 PositiveStrandReadsAssigned:275066 NegativeStrandReadsAssigned:4749529 Dataset is classified negative stranded MeadianReadLen=151 20thPercentileLength=145 echo kmer=141 SRR6941573 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,972 [index] number of k-mers: 66,720,672 [index] number of equivalence classes: 111,837 [quant] running in paired-end mode [quant] will process pair 1: SRR6941573-trimmed-pair1.fastq SRR6941573-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 36,715,783 reads, 12,057,436 reads pseudoaligned [quant] estimated average fragment length: 196.098 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,316 rounds 52973 SRR6941573.ke.tsv 35125 SRR6941573.se.tsv 88098 total ==> SRR6941573.ke.tsv <== target_id length eff_length est_counts tpm PNS24245 936 741.164 0 0 PNS24247 1044 848.902 2.62486 0.120556 PNS24249 1928 1732.9 14.1834 0.319115 PNS24246 1044 848.902 2.62486 0.120556 PNS24248 1044 848.902 2.62486 0.120556 PNS24244 1471 1275.9 7.94205 0.242693 PNS24243 293 116.738 0 0 KQK14069 1603 1407.9 340.766 9.43681 KQK14071 474 282.585 43.7449 6.03559 ==> SRR6941573.se.tsv <== BRADI_1g14170v3 494 BRADI_1g53295v3 14 BRADI_1g59795v3 19 BRADI_1g07683v3 0 BRADI_1g00485v3 0 BRADI_1g20270v3 22 BRADI_1g74790v3 13 BRADI_1g09890v3 0 BRADI_1g77505v3 20 BRADI_1g48960v3 0 SRR6941573 completed mapping pipeline successfully