Starting /dee2/code/volunteer_pipeline.sh SRR6941576 current disk space = 1551392059392 free memory = 1600574748 SRR6941576 SRAfilesize a0c47086f8bdaf4987e337691092faca SRR6941576.sra SRR6941576.sra file validated SRR6941576 is paired end SRR6941576 is conventional basespace SRR6941576 read1 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR6941576_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 34.495 35.0 35.0 35.0 34.0 35.0 2 34.51075 35.0 35.0 35.0 35.0 35.0 3 34.6165 35.0 35.0 35.0 35.0 35.0 4 34.66 35.0 35.0 35.0 35.0 35.0 5 34.46975 35.0 35.0 35.0 35.0 35.0 6 39.37125 40.0 40.0 40.0 39.0 40.0 7 39.387 40.0 40.0 40.0 39.0 40.0 8 39.3025 40.0 40.0 40.0 39.0 40.0 9 39.41525 40.0 40.0 40.0 39.0 40.0 10-14 39.3477 40.0 40.0 40.0 39.0 40.0 15-19 39.2274 40.0 40.0 40.0 38.8 40.0 20-24 39.3607 40.0 40.0 40.0 39.0 40.0 25-29 39.33335 40.0 40.0 40.0 39.0 40.0 30-34 39.227050000000006 40.0 40.0 40.0 38.8 40.0 35-39 39.269600000000004 40.0 40.0 40.0 39.0 40.0 40-44 39.284400000000005 40.0 40.0 40.0 39.0 40.0 45-49 39.220099999999995 40.0 40.0 40.0 38.8 40.0 50-54 39.1558 40.0 40.0 40.0 38.8 40.0 55-59 39.15555 40.0 40.0 40.0 38.4 40.0 60-64 39.126250000000006 40.0 40.0 40.0 38.0 40.0 65-69 39.155 40.0 40.0 40.0 38.6 40.0 70-74 39.0921 40.0 40.0 40.0 38.0 40.0 75-79 39.0175 40.0 39.8 40.0 37.8 40.0 80-84 39.01845 40.0 40.0 40.0 38.0 40.0 85-89 38.858000000000004 40.0 39.0 40.0 37.0 40.0 90-94 38.92525 40.0 39.0 40.0 37.6 40.0 95-99 38.739549999999994 40.0 39.0 40.0 37.0 40.0 100-104 38.17605 39.4 38.6 39.8 35.8 39.8 105-109 38.8178 40.0 39.0 40.0 37.0 40.0 110-114 38.7481 40.0 39.0 40.0 36.6 40.0 115-119 38.7379 40.0 39.0 40.0 36.8 40.0 120-124 38.57555 40.0 39.0 40.0 36.2 40.0 125-129 38.4483 40.0 39.0 40.0 36.0 40.0 130-134 38.4108 40.0 39.0 40.0 36.0 40.0 135-139 38.32805 40.0 39.0 40.0 36.0 40.0 140-144 38.1635 40.0 39.0 40.0 35.4 40.0 145-149 37.770450000000004 40.0 39.0 40.0 34.8 40.0 150-151 35.199625 38.5 35.0 39.5 25.0 40.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 18 2.0 19 0.0 20 1.0 21 2.0 22 1.0 23 2.0 24 7.0 25 4.0 26 9.0 27 12.0 28 12.0 29 22.0 30 24.0 31 35.0 32 47.0 33 36.0 34 65.0 35 81.0 36 115.0 37 178.0 38 355.0 39 2990.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 47.99699549323986 12.669003505257887 7.536304456685028 31.797696544817228 2 23.244734202607823 14.393179538615847 31.41925777331996 30.94282848545637 3 22.55 20.275000000000002 25.224999999999998 31.95 4 25.35 26.35 22.900000000000002 25.4 5 23.900477506911283 33.249560191002764 23.498366423724555 19.351595878361397 6 19.275000000000002 39.0 22.15 19.575 7 14.075 31.900000000000002 38.5 15.525 8 17.224999999999998 29.125 31.275 22.375 9 17.424999999999997 26.450000000000003 34.125 22.0 10-14 18.705 34.760000000000005 25.679999999999996 20.855 15-19 19.55 32.165 26.215 22.07 20-24 17.98 32.17 27.400000000000002 22.45 25-29 21.805 32.464999999999996 26.57 19.16 30-34 22.650000000000002 33.445 24.255 19.650000000000002 35-39 20.49 33.315 25.974999999999998 20.22 40-44 19.245 32.5 26.240000000000002 22.015 45-49 19.220961048052402 31.84159207960398 28.171408570428518 20.766038301915096 50-54 20.47 32.324999999999996 26.3 20.905 55-59 20.919999999999998 31.405 25.35 22.325 60-64 18.43 32.885 27.02 21.665 65-69 19.56 33.285 25.145 22.009999999999998 70-74 19.705000000000002 32.385000000000005 24.474999999999998 23.435 75-79 20.44 31.365 26.595000000000002 21.6 80-84 22.189999999999998 30.915 25.019999999999996 21.875 85-89 20.76 31.31 26.155 21.775 90-94 19.075 31.724999999999998 26.834999999999997 22.365 95-99 20.16 31.77 24.21 23.86 100-104 19.415 33.375 25.35 21.86 105-109 19.725 32.445 25.759999999999998 22.07 110-114 20.48 30.495 26.33 22.695 115-119 19.695 32.7 24.69 22.915 120-124 19.375 31.674999999999997 24.77 24.18 125-129 19.27 33.085 24.044999999999998 23.599999999999998 130-134 20.875 33.39 24.07 21.665 135-139 22.21 32.485 24.205 21.099999999999998 140-144 22.745 31.240000000000002 25.445 20.57 145-149 20.29 32.79 24.65 22.27 150-151 19.96993234778251 33.56301678777249 23.264845903282385 23.202204961162614 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 3.0 1 2.0 2 0.5 3 0.0 4 0.0 5 0.5 6 1.5 7 1.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.5 19 1.0 20 2.5 21 4.0 22 3.0 23 2.0 24 3.5 25 3.0 26 5.0 27 10.5 28 13.0 29 19.0 30 24.5 31 28.0 32 34.0 33 36.5 34 42.5 35 53.5 36 103.0 37 239.0 38 303.0 39 278.5 40 306.5 41 338.5 42 281.0 43 274.5 44 272.5 45 235.0 46 200.5 47 165.0 48 155.0 49 101.0 50 64.0 51 45.5 52 37.5 53 34.0 54 32.5 55 30.5 56 32.5 57 29.5 58 23.5 59 21.5 60 18.0 61 16.0 62 15.0 63 8.5 64 11.0 65 17.0 66 7.5 67 1.0 68 2.5 69 2.0 70 1.0 71 1.5 72 1.0 73 0.0 74 0.0 75 0.0 76 0.0 77 0.5 78 0.5 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.15 2 0.3 3 0.0 4 0.0 5 0.525 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.005 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.22499999999999998 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.475 #Duplication Level Percentage of deduplicated Percentage of total 1 83.83629599007854 50.7 2 6.614303431169905 8.0 3 2.9764365440264573 5.4 4 1.1575031004547334 2.8000000000000003 5 1.7362546506821002 5.25 6 0.7027697395618024 2.55 7 0.6200909466721786 2.625 8 0.24803637866887143 1.2 9 0.37205456800330716 2.025 >10 1.694915254237288 17.8 >50 0.0413393964448119 1.6500000000000001 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GCGGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTACG 66 1.6500000000000001 No Hit CTTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCAT 43 1.075 No Hit GCTTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCA 42 1.05 No Hit GGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTACGTT 41 1.0250000000000001 No Hit CTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCATTAAT 33 0.8250000000000001 No Hit GCGGAAAAACCTTATCCATTAATAGCGGGAACTTCAAGAGCAGCTAGATC 26 0.65 No Hit GGATGCATAAGGATGTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAA 25 0.625 No Hit GGCCAAAATAACCATGAGCAGCCACAATATTATAAGTCTCTTCCTCTTGA 24 0.6 No Hit GTTAGCGGAAAAACCTTATCCATTAATAGCGGGAACTTCAAGAGCAGCTA 24 0.6 No Hit CCCAAGTATTAATAACGCGACCTTGACTATCAACTACAGATTGGTTGAAA 20 0.5 No Hit CTCATAAGGACCACCATTGTATAACCATTCATCAACGGATGCAGCTTCCC 20 0.5 No Hit GCGACCTTGACTATCAACTACAGATTGGTTGAAATTGAATCCATTTAGGT 19 0.475 No Hit GCATAAGGATGTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAAAGTA 19 0.475 No Hit AGCGGAAAAACCTTATCCATTAATAGCGGGAACTTCAAGAGCAGCTAGAT 19 0.475 No Hit CGGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTACGT 18 0.44999999999999996 No Hit GCCAAAATAACCATGAGCAGCCACAATATTATAAGTCTCTTCCTCTTGAC 18 0.44999999999999996 No Hit TTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCATT 17 0.42500000000000004 No Hit GTGCTCTGCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTCCTA 17 0.42500000000000004 No Hit GCTCTGCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTCCTAAA 17 0.42500000000000004 No Hit CTCTGCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTCCTAAAG 15 0.375 No Hit GACCCATATAACAAGCTACACCAAGTAAGAAGTGTAGAACAATTAGCTCA 14 0.35000000000000003 No Hit CCAGATTCCTACTACAGGCCAAGCAGCCAAGAAGAAGTGTAAAGAACGAG 13 0.325 No Hit GAGTTGTTGAAACTAGCATATTGGAAGATTAATCGGCCAAAATAACCATG 13 0.325 No Hit GCCCAAGTATTAATAACGCGACCTTGACTATCAACTACAGATTGGTTGAA 13 0.325 No Hit GTTGAAACTAGCATATTGGAAGATTAATCGGCCAAAATAACCATGAGCAG 13 0.325 No Hit GCCAAGAAGAAGTGTAAAGAACGAGAGTTGTTGAAACTAGCATATTGGAA 13 0.325 No Hit GTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTC 12 0.3 No Hit GTTGATGATATCAGCCCAAGTATTAATAACGCGACCTTGACTATCAACTA 12 0.3 No Hit CTCATTAGCAGATTCATTTTCAGTAGTTTCCCTGATCAAACTAGAGGTTA 12 0.3 No Hit CTCGCGAATACCATCAATATCTACTGGAGGGGCTGCGATGAAGGCGATAA 12 0.3 No Hit GGAGGGGCTGCGATGAAGGCGATAATAAATACAGAAGTTGCGGTCAATAA 11 0.27499999999999997 No Hit GTGAGCATTACGTTCGTGCATTACTTCCATACCAAGATTAGCACGGTTGA 11 0.27499999999999997 No Hit CCCTCATTAGCAGATTCATTTTCAGTAGTTTCCCTGATCAAACTAGAGGT 11 0.27499999999999997 No Hit ATCAATATCTACTGGAGGGGCTGCGATGAAGGCGATAATAAATACAGAAG 11 0.27499999999999997 No Hit CTCTTGACCAAATTTGTAACCCTCATTAGCAGATTCATTTTCAGTAGTTT 11 0.27499999999999997 No Hit GCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTCCTAAAGGCAT 11 0.27499999999999997 No Hit GGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTACGTTC 11 0.27499999999999997 No Hit CGCGACCTTGACTATCAACTACAGATTGGTTGAAATTGAATCCATTTAGG 11 0.27499999999999997 No Hit GCACGGTTGATGATATCAGCCCAAGTATTAATAACGCGACCTTGACTATC 10 0.25 No Hit GCGGTCAATAAGGTAGGGATCATCAAAACACCGAACCATCCGATGTAAAG 10 0.25 No Hit GCATATTGGAAGATTAATCGGCCAAAATAACCATGAGCAGCCACAATATT 10 0.25 No Hit CATATAACAAGCTACACCAAGTAAGAAGTGTAGAACAATTAGCTCATAAG 10 0.25 No Hit GTGCAATCCGATTGCCGCAGAAGTAGGAATAATAGCACCAGAGATAATAT 9 0.22499999999999998 No Hit CCCATATAACAAGCTACACCAAGTAAGAAGTGTAGAACAATTAGCTCATA 9 0.22499999999999998 No Hit ATCCGATTGCCGCAGAAGTAGGAATAATAGCACCAGAGATAATATTGTTT 9 0.22499999999999998 No Hit GGGAAGTTGTGAGCATTACGTTCGTGCATTACTTCCATACCAAGATTAGC 9 0.22499999999999998 No Hit CCACCATTGTATAACCATTCATCAACGGATGCAGCTTCCCAAATTGGGTA 9 0.22499999999999998 No Hit TTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCATTAATA 9 0.22499999999999998 No Hit ACGCGACCTTGACTATCAACTACAGATTGGTTGAAATTGAATCCATTTAG 9 0.22499999999999998 No Hit GCTAGATCTAGAGGGAAGTTGTGAGCATTACGTTCGTGCATTACTTCCAT 9 0.22499999999999998 No Hit CATTAATAGCGGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGA 9 0.22499999999999998 No Hit GGAAAAACCTTATCCATTAATAGCGGGAACTTCAAGAGCAGCTAGATCTA 8 0.2 No Hit CGAGAGTTGTTGAAACTAGCATATTGGAAGATTAATCGGCCAAAATAACC 8 0.2 No Hit TTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCATTA 8 0.2 No Hit CATCAGAGAAGCTTCCTTGACCAATAGGGTAAATCAAGAAAACAGCAGTC 8 0.2 No Hit GTAAAGAACGAGAGTTGTTGAAACTAGCATATTGGAAGATTAATCGGCCA 8 0.2 No Hit CAGATTCCTACTACAGGCCAAGCAGCCAAGAAGAAGTGTAAAGAACGAGA 8 0.2 No Hit CGCCATAGTACTAATACCTAAAGCAGTGAACCAGATTCCTACTACAGGCC 7 0.17500000000000002 No Hit GGAAGATTAATCGGCCAAAATAACCATGAGCAGCCACAATATTATAAGTC 7 0.17500000000000002 No Hit CTTCCTCTTGACCAAATTTGTAACCCTCATTAGCAGATTCATTTTCAGTA 7 0.17500000000000002 No Hit CTTGACTATCAACTACAGATTGGTTGAAATTGAATCCATTTAGGTTGAAC 7 0.17500000000000002 No Hit TGATGATATCAGCCCAAGTATTAATAACGCGACCTTGACTATCAACTACA 7 0.17500000000000002 No Hit GCATTACGTTCGTGCATTACTTCCATACCAAGATTAGCACGGTTGATGAT 7 0.17500000000000002 No Hit GTTGTTGAAACTAGCATATTGGAAGATTAATCGGCCAAAATAACCATGAG 7 0.17500000000000002 No Hit GCTAGTTATCCAGTTGCAGAAGCGACCCCACAGGCTTGTACTTTCGCGTC 7 0.17500000000000002 No Hit CTAACATGTGAAATGGATGCATAAGGATGTTGTGCTCTGCCTGGAATACA 7 0.17500000000000002 No Hit GTAGGGATCATCAAAACACCGAACCATCCGATGTAAAGACGATTTTCAGT 7 0.17500000000000002 No Hit GTTCGCTGGAGTAGGCTGCGAGGCCCAAGCGGCCAAGGAGTGCAGCGGCC 7 0.17500000000000002 No Hit GGCCAAGCAGCCAAGAAGAAGTGTAAAGAACGAGAGTTGTTGAAACTAGC 7 0.17500000000000002 No Hit GATGCATAAGGATGTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAAA 7 0.17500000000000002 No Hit CATCAATATCTACTGGAGGGGCTGCGATGAAGGCGATAATAAATACAGAA 7 0.17500000000000002 No Hit CACCATTGTATAACCATTCATCAACGGATGCAGCTTCCCAAATTGGGTAA 7 0.17500000000000002 No Hit GTGTAAAGAACGAGAGTTGTTGAAACTAGCATATTGGAAGATTAATCGGC 6 0.15 No Hit CAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCATTAATAGCGGGAA 6 0.15 No Hit GCCGAAAACACCAGCTACACCTAACATGTGAAATGGATGCATAAGGATGT 6 0.15 No Hit GGCGCATACCCAAACGGAAACTAAGTTCCCACTCACGACCCATATAACAA 6 0.15 No Hit GTCGCAGCTGCAACAGGAGCTGAATATGCAACAGCAATCCAAGGGCGCAT 6 0.15 No Hit GAGGGAAGTTGTGAGCATTACGTTCGTGCATTACTTCCATACCAAGATTA 6 0.15 No Hit TCTTGACCAAATTTGTAACCCTCATTAGCAGATTCATTTTCAGTAGTTTC 6 0.15 No Hit GCTGCGATGAAGGCGATAATAAATACAGAAGTTGCGGTCAATAAGGTAGG 6 0.15 No Hit CGGAAAAACCTTATCCATTAATAGCGGGAACTTCAAGAGCAGCTAGATCT 6 0.15 No Hit CCTTGACTATCAACTACAGATTGGTTGAAATTGAATCCATTTAGGTTGAA 6 0.15 No Hit TGCATAAGGATGTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAAAGT 6 0.15 No Hit CTTATATGTTAGCGGAAAAACCTTATCCATTAATAGCGGGAACTTCAAGA 6 0.15 No Hit CCACTCACGACCCATATAACAAGCTACACCAAGTAAGAAGTGTAGAACAA 6 0.15 No Hit CATAAGGATGTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAAAGTAC 6 0.15 No Hit GTTGAAATTGAATCCATTTAGGTTGAACGCCATAGTACTAATACCTAAAG 6 0.15 No Hit GGTAAATCAAGAAAACAGCAGTCGCAGCTGCAACAGGAGCTGAATATGCA 6 0.15 No Hit CCTCATTAGCAGATTCATTTTCAGTAGTTTCCCTGATCAAACTAGAGGTT 6 0.15 No Hit CGCGAATACCATCAATATCTACTGGAGGGGCTGCGATGAAGGCGATAATA 5 0.125 No Hit ACCAGCTACACCTAACATGTGAAATGGATGCATAAGGATGTTGTGCTCTG 5 0.125 No Hit GCGATAATAAATACAGAAGTTGCGGTCAATAAGGTAGGGATCATCAAAAC 5 0.125 No Hit CCGCAGAAGTAGGAATAATAGCACCAGAGATAATATTGTTTCCATAAAGT 5 0.125 No Hit ATCAGAGAAGCTTCCTTGACCAATAGGGTAAATCAAGAAAACAGCAGTCG 5 0.125 No Hit ACCAGATATTCCTAAAGGCATACCATCAGAGAAGCTTCCTTGACCAATAG 5 0.125 No Hit GGTTGATGATATCAGCCCAAGTATTAATAACGCGACCTTGACTATCAACT 5 0.125 No Hit CTACTACAGGCCAAGCAGCCAAGAAGAAGTGTAAAGAACGAGAGTTGTTG 5 0.125 No Hit GGTAAAAGTGCAATCCGATTGCCGCAGAAGTAGGAATAATAGCACCAGAG 5 0.125 No Hit GTCGCAGAAGTAGGAATAATAGCACCAGAGATAATATTGTTTCCATAAAG 5 0.125 No Hit GTTGCGGTCAATAAGGTAGGGATCATCAAAACACCGAACCATCCGATGTA 5 0.125 No Hit CCAAGAAGAAGTGTAAAGAACGAGAGTTGTTGAAACTAGCATATTGGAAG 5 0.125 No Hit GGTCGAACTACCAGAATGTCTAGAAATGTAGAGCTTAAACTAGAAAGGCT 5 0.125 No Hit GCAGTGAACCAGATTCCTACTACAGGCCAAGCAGCCAAGAAGAAGTGTAA 5 0.125 No Hit GGGCTGCGATGAAGGCGATAATAAATACAGAAGTTGCGGTCAATAAGGTA 5 0.125 No Hit GGTCAATAAGGTAGGGATCATCAAAACACCGAACCATCCGATGTAAAGAC 5 0.125 No Hit GGCCCATATAACAAGCTACACCAAGTAAGAAGTGTAGAACAATTAGCTCA 5 0.125 No Hit GGCTGCGATGAAGGCGATAATAAATACAGAAGTTGCGGTCAATAAGGTAG 5 0.125 No Hit GTAGAACAATTAGCTCATAAGGACCACCATTGTATAACCATTCATCAACG 5 0.125 No Hit AGCGGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTAC 5 0.125 No Hit CCACAATATTATAAGTCTCTTCCTCTTGACCAAATTTGTAACCCTCATTA 5 0.125 No Hit CCAAGTATTAATAACGCGACCTTGACTATCAACTACAGATTGGTTGAAAT 5 0.125 No Hit GAAAAACCTTATCCATTAATAGCGGGAACTTCAAGAGCAGCTAGATCTAG 5 0.125 No Hit GTTTCCATAAAGTAAAGAACCAGAAACAGGCTCGCGAATACCATCAATAT 5 0.125 No Hit CTAGCATATTGGAAGATTAATCGGCCAAAATAACCATGAGCAGCCACAAT 5 0.125 No Hit GTGCTAGTTATCCAGTTGCAGAAGCGACCCCACAGGCTTGTACTTTCGCG 5 0.125 No Hit ATATAACAAGCTACACCAAGTAAGAAGTGTAGAACAATTAGCTCATAAGG 5 0.125 No Hit CCAGAGATAATATTGTTTCCATAAAGTAAAGAACCAGAAACAGGCTCGCG 5 0.125 No Hit TAAAGAACGAGAGTTGTTGAAACTAGCATATTGGAAGATTAATCGGCCAA 5 0.125 No Hit CGGTCAATAAGGTAGGGATCATCAAAACACCGAACCATCCGATGTAAAGA 5 0.125 No Hit GTAGGAATAATAGCACCAGAGATAATATTGTTTCCATAAAGTAAAGAACC 5 0.125 No Hit GTGCATTACTTCCATACCAAGATTAGCACGGTTGATGATATCAGCCCAAG 5 0.125 No Hit GTTCGTGCATTACTTCCATACCAAGATTAGCACGGTTGATGATATCAGCC 5 0.125 No Hit CCATGAGCAGCCACAATATTATAAGTCTCTTCCTCTTGACCAAATTTGTA 5 0.125 No Hit CTGGAATACAATCATAAAGTTGAAAGTACCAGATATTCCTAAAGGCATAC 5 0.125 No Hit AGCAGATTCATTTTCAGTAGTTTCCCTGATCAAACTAGAGGTTACCAAGG 5 0.125 No Hit CGGCCAAAATAACCATGAGCAGCCACAATATTATAAGTCTCTTCCTCTTG 5 0.125 No Hit GTCAATAAGGTAGGGATCATCAAAACACCGAACCATCCGATGTAAAGACG 5 0.125 No Hit GGGTAAAAGTGCAATCCGATTGCCGCAGAAGTAGGAATAATAGCACCAGA 5 0.125 No Hit GAGCATTACGTTCGTGCATTACTTCCATACCAAGATTAGCACGGTTGATG 5 0.125 No Hit ATAACAAGCTACACCAAGTAAGAAGTGTAGAACAATTAGCTCATAAGGAC 5 0.125 No Hit CTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCATTAATAGCGG 5 0.125 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0125 0.0 0.0 0.0 0.0 64-65 0.025 0.0 0.0 0.0 0.0 66-67 0.05 0.0 0.0 0.0 0.0 68-69 0.05 0.0 0.0 0.0 0.0 70-71 0.0625 0.0 0.0 0.0 0.0 72-73 0.1125 0.0 0.0 0.0 0.0 74-75 0.125 0.0 0.0 0.0 0.0 76-77 0.1375 0.0 0.0 0.0 0.0 78-79 0.16249999999999998 0.0 0.0 0.0 0.0 80-81 0.2 0.0 0.0 0.0 0.0 82-83 0.25 0.0 0.0 0.0 0.0 84-85 0.275 0.0 0.0 0.0 0.0 86-87 0.275 0.0 0.0 0.0 0.0 88-89 0.35 0.0 0.0 0.0 0.0 90-91 0.4125 0.0 0.0 0.0 0.0 92-93 0.475 0.0 0.0 0.0 0.0 94-95 0.625 0.0 0.0 0.0 0.0 96-97 0.775 0.0 0.0 0.0 0.0 98-99 0.8625 0.0 0.0 0.0 0.0 100-101 1.1124999999999998 0.0 0.0 0.0 0.0 102-103 1.2875 0.0 0.0 0.0 0.0 104-105 1.425 0.0 0.0 0.0 0.0 106-107 1.5499999999999998 0.0 0.0 0.0 0.0 108-109 1.7374999999999998 0.0 0.0 0.0 0.0 110-111 1.9749999999999999 0.0 0.0 0.0 0.0 112-113 2.2375 0.0 0.0 0.0 0.0 114-115 2.5 0.0 0.0 0.0 0.0 116-117 2.7750000000000004 0.0 0.0 0.0 0.0 118-119 3.05 0.0 0.0 0.0 0.0 120-121 3.3375000000000004 0.0 0.0 0.0 0.0 122-123 3.675 0.0 0.0 0.0 0.0 124-125 3.9125 0.0 0.0 0.0 0.0 126-127 4.2125 0.0 0.0 0.0 0.0 128-129 4.4375 0.0 0.0 0.0 0.0 130-131 4.75 0.0 0.0 0.0 0.0 132-133 5.2625 0.0 0.0 0.0 0.0 134-135 5.7375 0.0 0.0 0.0 0.0 136-137 6.2125 0.0 0.0 0.0 0.0 138-139 6.7375 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CAAAAAT 30 1.4118372E-5 96.666664 9 CTTTTCT 25 8.7132835E-4 87.0 1 CTTCAAA 25 8.7132835E-4 87.0 6 TCTTCAA 25 8.7132835E-4 87.0 5 TTTCTTC 30 0.0017973486 72.5 3 TTCAAAA 30 0.0017973486 72.5 7 TTCTTCA 30 0.0017973486 72.5 4 TCAAAAA 35 0.0033124194 62.14286 8 ATTGAAT 40 0.005621335 54.375 145 TTTTCTT 45 0.008957279 48.333332 2 >>END_MODULE SRR6941576 read2 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR6941576_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 34.0575 35.0 35.0 35.0 32.0 35.0 2 34.30925 35.0 35.0 35.0 33.0 35.0 3 34.2775 35.0 35.0 35.0 33.0 35.0 4 34.171 35.0 35.0 35.0 33.0 35.0 5 34.3025 35.0 35.0 35.0 33.0 35.0 6 38.909 40.0 40.0 40.0 38.0 40.0 7 38.87775 40.0 40.0 40.0 38.0 40.0 8 38.9885 40.0 40.0 40.0 38.0 40.0 9 38.9905 40.0 40.0 40.0 38.0 40.0 10-14 38.94884999999999 40.0 40.0 40.0 38.6 40.0 15-19 39.030249999999995 40.0 40.0 40.0 39.0 40.0 20-24 38.96935 40.0 40.0 40.0 38.6 40.0 25-29 38.964749999999995 40.0 40.0 40.0 38.2 40.0 30-34 38.92265 40.0 39.8 40.0 38.2 40.0 35-39 38.9587 40.0 40.0 40.0 38.0 40.0 40-44 38.831649999999996 40.0 39.8 40.0 37.8 40.0 45-49 38.7276 40.0 39.4 40.0 37.2 40.0 50-54 38.66015 40.0 39.0 40.0 37.0 40.0 55-59 38.6997 40.0 39.0 40.0 37.0 40.0 60-64 38.71265 40.0 39.0 40.0 37.0 40.0 65-69 38.58075 40.0 39.0 40.0 36.6 40.0 70-74 38.5941 40.0 39.0 40.0 36.4 40.0 75-79 38.376599999999996 40.0 39.0 40.0 35.8 40.0 80-84 38.3899 40.0 39.0 40.0 36.0 40.0 85-89 38.326299999999996 40.0 39.0 40.0 36.0 40.0 90-94 38.28365 40.0 39.0 40.0 36.0 40.0 95-99 38.1945 40.0 39.0 40.0 35.6 40.0 100-104 37.315 39.0 38.0 39.6 32.8 39.8 105-109 37.92035 40.0 39.0 40.0 34.8 40.0 110-114 34.60595 36.4 34.2 38.4 30.2 38.6 115-119 18.805699999999998 16.6 15.8 23.4 13.4 30.0 120-124 2.0 2.0 2.0 2.0 2.0 2.0 125-129 2.0 2.0 2.0 2.0 2.0 2.0 130-134 2.0 2.0 2.0 2.0 2.0 2.0 135-139 2.0 2.0 2.0 2.0 2.0 2.0 140-144 2.0 2.0 2.0 2.0 2.0 2.0 145-149 2.0 2.0 2.0 2.0 2.0 2.0 150-151 2.0 2.0 2.0 2.0 2.0 2.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 9.0 3 2.0 4 0.0 5 1.0 6 0.0 7 1.0 8 0.0 9 3.0 10 0.0 11 2.0 12 1.0 13 1.0 14 5.0 15 4.0 16 9.0 17 4.0 18 6.0 19 23.0 20 14.0 21 16.0 22 20.0 23 41.0 24 36.0 25 59.0 26 84.0 27 105.0 28 143.0 29 301.0 30 1979.0 31 1131.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 37.69095917856248 20.135236664162285 17.45554720761332 24.718256949661907 2 21.97747183979975 23.90488110137672 37.622027534418024 16.49561952440551 3 19.86986986986987 25.025025025025027 39.16416416416417 15.94094094094094 4 21.051314142678347 29.86232790988736 29.18648310387985 19.899874843554443 5 22.453066332916144 30.663329161451813 29.812265331664577 17.07133917396746 6 19.375 32.550000000000004 30.049999999999997 18.025 7 17.549999999999997 20.8 43.475 18.175 8 19.5 25.15 32.425 22.925 9 22.075 20.925 35.35 21.65 10-14 22.17 25.615 32.835 19.38 15-19 22.495 25.41 33.019999999999996 19.075 20-24 22.725 25.06 33.365 18.85 25-29 22.39 25.590000000000003 32.324999999999996 19.695 30-34 23.455000000000002 24.665 32.59 19.29 35-39 23.158473771065662 24.318647797169575 32.42486372955943 20.09801470220533 40-44 21.54146243873162 25.802740822246673 32.424727418225466 20.231069320796237 45-49 21.895653915481674 26.106549168836366 32.230122171039454 19.7676747446425 50-54 22.162730184147318 25.890712570056046 32.44595676541233 19.500600480384307 55-59 21.968673372366514 26.317369764299652 31.331631887104038 20.382324976229796 60-64 22.13217269498224 25.203862124168293 32.77802791535345 19.885937265496022 65-69 22.0293190573873 25.506579276529745 31.75063791464452 20.713463751438436 70-74 22.563538122873723 25.485291174704823 32.2643586151691 19.68681208725235 75-79 23.308496274441165 24.8587288093214 32.439865979896986 19.39290893634045 80-84 23.1627395067287 24.74861173645505 33.67352043623993 18.41512832057632 85-89 23.83357503625544 25.92888933340001 31.14467170075511 19.092863929589438 90-94 22.689999999999998 25.455 31.490000000000002 20.365 95-99 22.52 26.369999999999997 30.740000000000002 20.369999999999997 100-104 22.761138056902848 26.036301815090756 32.27661383069154 18.925946297314866 105-109 23.32 24.3 32.235 20.145 110-114 23.45604808414726 25.103931880791386 31.525169045830204 19.91485098923115 115-119 22.411196184260074 26.26459143968872 31.793648801305384 19.530563574745827 120-124 23.432050225125245 40.61766757562306 19.86175407444987 16.088528124801826 125-129 NaN NaN NaN NaN 130-134 22.033898305084744 26.60389202762084 30.772128060263654 20.59008160703076 135-139 23.143688541039534 26.11880393337347 31.662653020268916 19.074854505318083 140-144 23.416325244248345 26.870469587141503 31.459186889379136 18.254018279231012 145-149 21.99149362021516 25.69427070302727 34.00050037528146 18.313735301476108 150-151 23.042225285051998 25.09710562586142 33.34168650545045 18.518982583636138 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1.0 1 1.5 2 1.0 3 0.0 4 0.0 5 0.5 6 1.0 7 0.5 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.5 18 0.5 19 0.0 20 0.5 21 1.5 22 2.5 23 2.5 24 3.5 25 6.0 26 11.5 27 18.5 28 24.5 29 31.0 30 33.5 31 36.5 32 40.0 33 61.5 34 99.0 35 105.5 36 113.0 37 183.5 38 229.5 39 231.0 40 269.5 41 321.0 42 281.0 43 255.0 44 279.0 45 232.0 46 207.0 47 167.0 48 113.5 49 94.5 50 80.0 51 78.0 52 57.0 53 34.0 54 35.5 55 38.5 56 34.0 57 27.0 58 21.0 59 20.5 60 19.0 61 16.0 62 11.0 63 10.0 64 16.0 65 17.0 66 10.0 67 4.0 68 3.5 69 3.0 70 1.5 71 1.0 72 0.0 73 0.0 74 1.0 75 1.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 0.17500000000000002 2 0.125 3 0.1 4 0.125 5 0.125 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.015 40-44 0.03 45-49 0.13999999999999999 50-54 0.08 55-59 0.08499999999999999 60-64 0.055 65-69 0.065 70-74 0.06 75-79 0.015 80-84 0.055 85-89 0.015 90-94 0.0 95-99 0.0 100-104 0.005 105-109 0.0 110-114 0.17500000000000002 115-119 20.330000000000002 120-124 21.154999999999998 125-129 100.0 130-134 60.175 135-139 0.33999999999999997 140-144 20.674999999999997 145-149 80.015 150-151 0.2375 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 63.025 #Duplication Level Percentage of deduplicated Percentage of total 1 81.7929393097977 51.55 2 7.417691392304642 9.35 3 3.5700119000396673 6.75 4 1.9833399444664817 5.0 5 1.586671955573185 5.0 6 1.0710035700119 4.05 7 0.4760015866719556 2.1 8 0.3570011900039667 1.7999999999999998 9 0.39666798889329624 2.25 >10 1.3486711622372074 12.15 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source ATTATCTATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAAT 32 0.8 No Hit TATCTATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTT 28 0.7000000000000001 No Hit CTATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAA 22 0.5499999999999999 No Hit ATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAA 20 0.5 No Hit GTTATTTTGGCCGATTAATCTTCCAATATGCTAGTTTCAACAACTCTCGT 19 0.475 No Hit GCTAGTTTCAACAACTCTCGTTCTTTACACTTCTTCTTGGCTGCTTGGCC 18 0.44999999999999996 No Hit GTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGATCTTACCATGAC 18 0.44999999999999996 No Hit ATCTATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTG 18 0.44999999999999996 No Hit GCTTGTTATATGGGTCGTGAGTGGGAACTTAGTTTCCGTTTGGGTATGCG 16 0.4 No Hit CAGGGAAACTACTGAAAATGAATCTGCTAATGAGGGTTACAAATTTGGTC 15 0.375 No Hit GTTAGGTGTAGCTGGTGTTTTCGGCGGTTCCCTATTCAGTGCTATGCATG 15 0.375 No Hit CTTCCAATATGCTAGTTTCAACAACTCTCGTTCTTTACACTTCTTCTTGG 15 0.375 No Hit CTGAAAATGAATCTGCTAATGAGGGTTACAAATTTGGTCAAGAGGAAGAG 15 0.375 No Hit CTGCTAATGAGGGTTACAAATTTGGTCAAGAGGAAGAGACTTATAATATT 14 0.35000000000000003 No Hit GGGAAACTACTGAAAATGAATCTGCTAATGAGGGTTACAAATTTGGTCAA 13 0.325 No Hit GTGGGAACTTAGTTTCCGTTTGGGTATGCGCCCTTGGATTGCTGTTGCAT 13 0.325 No Hit GTTTCAACAACTCTCGTTCTTTACACTTCTTCTTGGCTGCTTGGCCTGTA 13 0.325 No Hit GCACAACATCCTTATGCATCCATTTCACATGTTAGGTGTAGCTGGTGTTT 13 0.325 No Hit CCTAAATGGATTCAATTTCAACCAATCTGTAGTTGATAGTCAAGGTCGCG 12 0.3 No Hit GGAAGCTTCTCTGATGGTATGCCTTTAGGAATATCTGGTACTTTCAACTT 12 0.3 No Hit TCTATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGA 12 0.3 No Hit CAACATCCTTATGCATCCATTTCACATGTTAGGTGTAGCTGGTGTTTTCG 12 0.3 No Hit CTCTAGTTTGATCAGGGAAACTACTGAAAATGAATCTGCTAATGAGGGTT 11 0.27499999999999997 No Hit GGAAGAGACTTATAATATTGTGGCTGCTCATGGTTATTTTGGCCGATTAA 10 0.25 No Hit GGTTATTTTGGCCGATTAATCTTCCAATATGCTAGTTTCAACAACTCTCG 10 0.25 No Hit GTTATACAATGGTGGTCCTTATGAGCTAATTGTTCTACACTTCTTACTTG 10 0.25 No Hit CTAGCACTGAAAATCGTCTTTACATCGGATGGTTCGGTGTTTTGATGATC 10 0.25 No Hit GTAGGAATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCT 10 0.25 No Hit ATTCAGTGCTATGCATGGTTCCTTGGTAACCTCTAGTTTGATCAGGGAAA 10 0.25 No Hit GCTGCATCCGTTGATGAATGGTTATACAATGGTGGTCCTTATGAGCTAAT 10 0.25 No Hit GTTCAACCTAAATGGATTCAATTTCAACCAATCTGTAGTTGATAGTCAAG 10 0.25 No Hit GTTTGATCAGGGAAACTACTGAAAATGAATCTGCTAATGAGGGTTACAAA 10 0.25 No Hit GGTACTTTCAACTTTATGATTGTATTCCAGGCAGAGCACAACATCCTTAT 10 0.25 No Hit GTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGATCTTACCATGACTG 10 0.25 No Hit CTATTCAGTGCTATGCATGGTTCCTTGGTAACCTCTAGTTTGATCAGGGA 9 0.22499999999999998 No Hit GCTCATGGTTATTTTGGCCGATTAATCTTCCAATATGCTAGTTTCAACAA 9 0.22499999999999998 No Hit CTGGTACTTTCAACTTTATGATTGTATTCCAGGCAGAGCACAACATCCTT 9 0.22499999999999998 No Hit GGAAACTACTGAAAATGAATCTGCTAATGAGGGTTACAAATTTGGTCAAG 9 0.22499999999999998 No Hit GTTCCTTGGTAACCTCTAGTTTGATCAGGGAAACTACTGAAAATGAATCT 9 0.22499999999999998 No Hit CTTCTTCTTGGCTGCTTGGCCTGTAGTAGGAATCTGGTTCACTGCTTTAG 9 0.22499999999999998 No Hit CGCAGCCCCTCCAGTAGATATTGATGGTATTCGCGAGCCTGTTTCTGGTT 9 0.22499999999999998 No Hit GTTACAAATTTGGTCAAGAGGAAGAGACTTATAATATTGTGGCTGCTCAT 9 0.22499999999999998 No Hit GCTGCGACTGCTGTTTTCTTGATTTACCCTATTGGTCAAGGAAGCTTCTC 9 0.22499999999999998 No Hit AGGGAAACTACTGAAAATGAATCTGCTAATGAGGGTTACAAATTTGGTCA 9 0.22499999999999998 No Hit AAATTTGGTCAAGAGGAAGAGACTTATAATATTGTGGCTGCTCATGGTTA 8 0.2 No Hit CAACTCTCGTTCTTTACACTTCTTCTTGGCTGCTTGGCCTGTAGTAGGAA 8 0.2 No Hit TATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAAT 8 0.2 No Hit GCATGGTTCCTTGGTAACCTCTAGTTTGATCAGGGAAACTACTGAAAATG 8 0.2 No Hit ATTTCACATGTTAGGTGTAGCTGGTGTTTTCGGCGGTTCCCTATTCAGTG 8 0.2 No Hit CAGTGCTATGCATGGTTCCTTGGTAACCTCTAGTTTGATCAGGGAAACTA 8 0.2 No Hit GAGGAAGAGACTTATAATATTGTGGCTGCTCATGGTTATTTTGGCCGATT 8 0.2 No Hit CGAAAGTACAAGCCTGTGGGGTCGCTTCTGCAACTGGATAACTAGCACTG 8 0.2 No Hit TGGTTATACAATGGTGGTCCTTATGAGCTAATTGTTCTACACTTCTTACT 8 0.2 No Hit GTTTTCTTGATTTACCCTATTGGTCAAGGAAGCTTCTCTGATGGTATGCC 7 0.17500000000000002 No Hit ATCGGATTGCACTTTTACCCAATTTGGGAAGCTGCATCCGTTGATGAATG 7 0.17500000000000002 No Hit CTATTATCTATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCA 7 0.17500000000000002 No Hit CGTTGATGAATGGTTATACAATGGTGGTCCTTATGAGCTAATTGTTCTAC 7 0.17500000000000002 No Hit GACTTATAATATTGTGGCTGCTCATGGTTATTTTGGCCGATTAATCTTCC 7 0.17500000000000002 No Hit GCTAATGAGGGTTACAAATTTGGTCAAGAGGAAGAGACTTATAATATTGT 7 0.17500000000000002 No Hit AACAACTCTCGTTCTTTACACTTCTTCTTGGCTGCTTGGCCTGTAGTAGG 7 0.17500000000000002 No Hit TGAGTGGGAACTTAGTTTCCGTTTGGGTATGCGCCCTTGGATTGCTGTTG 7 0.17500000000000002 No Hit CTTAGTTTCCGTTTGGGTATGCGCCCTTGGATTGCTGTTGCATATTCAGC 7 0.17500000000000002 No Hit TAATCTTCCAATATGCTAGTTTCAACAACTCTCGTTCTTTACACTTCTTC 7 0.17500000000000002 No Hit GTCAAGGTCGCGTTATTAATACTTGGGCTGATATCATCAACCGTGCTAAT 7 0.17500000000000002 No Hit GAGCACAACATCCTTATGCATCCATTTCACATGTTAGGTGTAGCTGGTGT 7 0.17500000000000002 No Hit CGGCGGTTCCCTATTCAGTGCTATGCATGGTTCCTTGGTAACCTCTAGTT 6 0.15 No Hit GTTTGGGTATGCGCCCTTGGATTGCTGTTGCATATTCAGCTCCTGTTGCA 6 0.15 No Hit AGGAATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCTAA 6 0.15 No Hit GTCTTTACATCGGATGGTTCGGTGTTTTGATGATCCCTACCTTATTGACC 6 0.15 No Hit CTCTCGTTCTTTACACTTCTTCTTGGCTGCTTGGCCTGTAGTAGGAATCT 6 0.15 No Hit ATTTCAACCAATCTGTAGTTGATAGTCAAGGTCGCGTTATTAATACTTGG 6 0.15 No Hit GTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGATCTT 6 0.15 No Hit GAGGGTTACAAATTTGGTCAAGAGGAAGAGACTTATAATATTGTGGCTGC 6 0.15 No Hit CCTATTCAGTGCTATGCATGGTTCCTTGGTAACCTCTAGTTTGATCAGGG 6 0.15 No Hit ATTATCTCTGGTGCTATTATTCCTACTTCTGCGGCAATCGGATTGCACTT 6 0.15 No Hit GTTCGATCCTGGCTCAGGATGAACGCTGGCGGCATGCTTAACACATGCAA 6 0.15 No Hit TTCCAATATGCTAGTTTCAACAACTCTCGTTCTTTACACTTCTTCTTGGC 6 0.15 No Hit GGAATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCTAAA 6 0.15 No Hit GTAGTTGATAGTCAAGGTCGCGTTATTAATACTTGGGCTGATATCATCAA 6 0.15 No Hit ATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCA 6 0.15 No Hit ATCTTCCAATATGCTAGTTTCAACAACTCTCGTTCTTTACACTTCTTCTT 6 0.15 No Hit CTTCTATTATCTATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTT 6 0.15 No Hit TGGGTCGTGAGTGGGAACTTAGTTTCCGTTTGGGTATGCGCCCTTGGATT 6 0.15 No Hit TGTAGCTGGTGTTTTCGGCGGTTCCCTATTCAGTGCTATGCATGGTTCCT 6 0.15 No Hit CAATGGTGGTCCTTATGAGCTAATTGTTCTACACTTCTTACTTGGTGTAG 6 0.15 No Hit GTTTCTGGTTCTTTACTTTATGGAAACAATATTATCTCTGGTGCTATTAT 6 0.15 No Hit CGGATGGTTCGGTGTTTTGATGATCCCTACCTTATTGACCGCAACTTCTG 6 0.15 No Hit CACATGTTAGGTGTAGCTGGTGTTTTCGGCGGTTCCCTATTCAGTGCTAT 6 0.15 No Hit TCTTCCAATATGCTAGTTTCAACAACTCTCGTTCTTTACACTTCTTCTTG 6 0.15 No Hit ATTGATGGTATTCGCGAGCCTGTTTCTGGTTCTTTACTTTATGGAAACAA 6 0.15 No Hit GAATAAACCAAGATCTTACCATGACTGCAATTTTAGAGAGACGCGAAAGT 6 0.15 No Hit AGCTTGTTATATGGGTCGTGAGTGGGAACTTAGTTTCCGTTTGGGTATGC 6 0.15 No Hit ACACTTCTTACTTGGTGTAGCTTGTTATATGGGTCGTGAGTGGGAACTTA 5 0.125 No Hit CCTAAATATAACATTAATACTTCAAGTTGCTGAGTTGAGAAAGAGATGGT 5 0.125 No Hit CCGCAACTTCTGTATTTATTATCGCCTTCATCGCAGCCCCTCCAGTAGAT 5 0.125 No Hit GGTAACCTCTAGTTTGATCAGGGAAACTACTGAAAATGAATCTGCTAATG 5 0.125 No Hit ATTTTGGCCGATTAATCTTCCAATATGCTAGTTTCAACAACTCTCGTTCT 5 0.125 No Hit TGTAGCTTGTTATATGGGTCGTGAGTGGGAACTTAGTTTCCGTTTGGGTA 5 0.125 No Hit GGTGTTTTGATGATCCCTACCTTATTGACCGCAACTTCTGTATTTATTAT 5 0.125 No Hit AGTACTATGGCGTTCAACCTAAATGGATTCAATTTCAACCAATCTGTAGT 5 0.125 No Hit GTACTATGGCGTTCAACCTAAATGGATTCAATTTCAACCAATCTGTAGTT 5 0.125 No Hit TAGGAATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCTA 5 0.125 No Hit GATTAATCTTCCAATATGCTAGTTTCAACAACTCTCGTTCTTTACACTTC 5 0.125 No Hit GAGACTTATAATATTGTGGCTGCTCATGGTTATTTTGGCCGATTAATCTT 5 0.125 No Hit GGCTGCTTGGCCTGTAGTAGGAATCTGGTTCACTGCTTTAGGTATTAGTA 5 0.125 No Hit TCGCAGCCCCTCCAGTAGATATTGATGGTATTCGCGAGCCTGTTTCTGGT 5 0.125 No Hit TGAAAATGAATCTGCTAATGAGGGTTACAAATTTGGTCAAGAGGAAGAGA 5 0.125 No Hit TTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAG 5 0.125 No Hit AGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGATCT 5 0.125 No Hit TGAAAATCGTCTTTACATCGGATGGTTCGGTGTTTTGATGATCCCTACCT 5 0.125 No Hit CTTCTTGGCTGCTTGGCCTGTAGTAGGAATCTGGTTCACTGCTTTAGGTA 5 0.125 No Hit GCTGTTTTCTTGATTTACCCTATTGGTCAAGGAAGCTTCTCTGATGGTAT 5 0.125 No Hit AAACAATATTATCTCTGGTGCTATTATTCCTACTTCTGCGGCAATCGGAT 5 0.125 No Hit GTTTTGATGATCCCTACCTTATTGACCGCAACTTCTGTATTTATTATCGC 5 0.125 No Hit ATTAGTACTATGGCGTTCAACCTAAATGGATTCAATTTCAACCAATCTGT 5 0.125 No Hit TTCAGTGCTATGCATGGTTCCTTGGTAACCTCTAGTTTGATCAGGGAAAC 5 0.125 No Hit GTAGCTTGTTATATGGGTCGTGAGTGGGAACTTAGTTTCCGTTTGGGTAT 5 0.125 No Hit CCCTATTCAGTGCTATGCATGGTTCCTTGGTAACCTCTAGTTTGATCAGG 5 0.125 No Hit CAAATTTGGTCAAGAGGAAGAGACTTATAATATTGTGGCTGCTCATGGTT 5 0.125 No Hit GTTGCATATTCAGCTCCTGTTGCAGCTGCGACTGCTGTTTTCTTGATTTA 5 0.125 No Hit GTAACCTCTAGTTTGATCAGGGAAACTACTGAAAATGAATCTGCTAATGA 5 0.125 No Hit GGTTCCTTGGTAACCTCTAGTTTGATCAGGGAAACTACTGAAAATGAATC 5 0.125 No Hit CCATTTCACATGTTAGGTGTAGCTGGTGTTTTCGGCGGTTCCCTATTCAG 5 0.125 No Hit GGTCAAGGAAGCTTCTCTGATGGTATGCCTTTAGGAATATCTGGTACTTT 5 0.125 No Hit GTACAAGCCTGTGGGGTCGCTTCTGCAACTGGATAACTAGCACTGAAAAT 5 0.125 No Hit ATCCATTTCACATGTTAGGTGTAGCTGGTGTTTTCGGCGGTTCCCTATTC 5 0.125 No Hit TGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCTAAATGGATT 5 0.125 No Hit GAATGGTTATACAATGGTGGTCCTTATGAGCTAATTGTTCTACACTTCTT 5 0.125 No Hit GATCCCTACCTTATTGACCGCAACTTCTGTATTTATTATCGCCTTCATCG 5 0.125 No Hit CTTTATGATTGTATTCCAGGCAGAGCACAACATCCTTATGCATCCATTTC 5 0.125 No Hit GTAGATATTGATGGTATTCGCGAGCCTGTTTCTGGTTCTTTACTTTATGG 5 0.125 No Hit GTTCTTTACTTTATGGAAACAATATTATCTCTGGTGCTATTATTCCTACT 5 0.125 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0125 0.0 0.0 0.0 0.0 64-65 0.025 0.0 0.0 0.0 0.0 66-67 0.05 0.0 0.0 0.0 0.0 68-69 0.05 0.0 0.0 0.0 0.0 70-71 0.0625 0.0 0.0 0.0 0.0 72-73 0.1125 0.0 0.0 0.0 0.0 74-75 0.125 0.0 0.0 0.0 0.0 76-77 0.1375 0.0 0.0 0.0 0.0 78-79 0.16249999999999998 0.0 0.0 0.0 0.0 80-81 0.1875 0.0 0.0 0.0 0.0 82-83 0.225 0.0 0.0 0.0 0.0 84-85 0.25 0.0 0.0 0.0 0.0 86-87 0.25 0.0 0.0 0.0 0.0 88-89 0.32499999999999996 0.0 0.0 0.0 0.0 90-91 0.3875 0.0 0.0 0.0 0.0 92-93 0.44999999999999996 0.0 0.0 0.0 0.0 94-95 0.6 0.0 0.0 0.0 0.0 96-97 0.75 0.0 0.0 0.0 0.0 98-99 0.8375 0.0 0.0 0.0 0.0 100-101 1.0875 0.0 0.0 0.0 0.0 102-103 1.2374999999999998 0.0 0.0 0.0 0.0 104-105 1.4 0.0 0.0 0.0 0.0 106-107 1.525 0.0 0.0 0.0 0.0 108-109 1.55 0.0 0.0 0.0 0.0 110-111 1.55 0.0 0.0 0.0 0.0 112-113 1.55 0.0 0.0 0.0 0.0 114-115 1.55 0.0 0.0 0.0 0.0 116-117 1.55 0.0 0.0 0.0 0.0 118-119 1.55 0.0 0.0 0.0 0.0 120-121 1.55 0.0 0.0 0.0 0.0 122-123 1.55 0.0 0.0 0.0 0.0 124-125 1.55 0.0 0.0 0.0 0.0 126-127 1.55 0.0 0.0 0.0 0.0 128-129 1.55 0.0 0.0 0.0 0.0 130-131 1.55 0.0 0.0 0.0 0.0 132-133 1.55 0.0 0.0 0.0 0.0 134-135 1.55 0.0 0.0 0.0 0.0 136-137 1.55 0.0 0.0 0.0 0.0 138-139 1.55 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTATATT 15 3.0800808E-4 113.0 4 ATCTATA 15 3.0800808E-4 113.0 2 TCTATAT 15 3.0800808E-4 113.0 3 TATATTA 20 9.6693676E-4 84.75 5 TATCTAT 20 9.6693676E-4 84.75 1 TTATTTC 25 0.002344875 67.8 9 ATTATTT 30 0.004829774 56.5 8 >>END_MODULE Read 1404472 spots for SRR6941576.sra Written 1404472 spots for SRR6941576.sra Read 1404472 spots for SRR6941576.sra Written 1404472 spots for SRR6941576.sra Read 1404472 spots for SRR6941576.sra Written 1404472 spots for SRR6941576.sra Read 1404472 spots for SRR6941576.sra Written 1404472 spots for SRR6941576.sra Read 1404472 spots for SRR6941576.sra Written 1404472 spots for SRR6941576.sra Read 1404472 spots for SRR6941576.sra Written 1404472 spots for SRR6941576.sra Read 1404472 spots for SRR6941576.sra Written 1404472 spots for SRR6941576.sra Read 1404472 spots for SRR6941576.sra Written 1404472 spots for SRR6941576.sra Read 1404472 spots for SRR6941576.sra Written 1404472 spots for SRR6941576.sra Read 1404472 spots for SRR6941576.sra Written 1404472 spots for SRR6941576.sra Read 1404472 spots for SRR6941576.sra Written 1404472 spots for SRR6941576.sra Read 1404472 spots for SRR6941576.sra Written 1404472 spots for SRR6941576.sra Read 1404472 spots for SRR6941576.sra Written 1404472 spots for SRR6941576.sra Read 1404472 spots for SRR6941576.sra Written 1404472 spots for SRR6941576.sra Read 1404472 spots for SRR6941576.sra Written 1404472 spots for SRR6941576.sra Read 1404472 spots for SRR6941576.sra Written 1404472 spots for SRR6941576.sra Read 1404472 spots for SRR6941576.sra Written 1404472 spots for SRR6941576.sra Read 1404472 spots for SRR6941576.sra Written 1404472 spots for SRR6941576.sra Read 1404483 spots for SRR6941576.sra Written 1404483 spots for SRR6941576.sra Read 1404472 spots for SRR6941576.sra Written 1404472 spots for SRR6941576.sra SRR ids: ['SRR6941576.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_sn9axx7w SRR6941576.sra spots: 28089451 blocks: [[1, 1404472], [1404473, 2808944], [2808945, 4213416], [4213417, 5617888], [5617889, 7022360], [7022361, 8426832], [8426833, 9831304], [9831305, 11235776], [11235777, 12640248], [12640249, 14044720], [14044721, 15449192], [15449193, 16853664], [16853665, 18258136], [18258137, 19662608], [19662609, 21067080], [21067081, 22471552], [22471553, 23876024], [23876025, 25280496], [25280497, 26684968], [26684969, 28089451]] SRR6941576 file size 9496892 SRR6941576 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR6941576 SRR6941576_1.fastq SRR6941576_2.fastq Input file: SRR6941576_1.fastq Paired file: SRR6941576_2.fastq trimmed: SRR6941576-trimmed-pair1.fastq, SRR6941576-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Fri Dec 6 11:53:16 2024 >> started Fri Dec 6 11:53:47 2024 >> done (31.048s) 28089451 read pairs processed; of these: 32773 ( 0.12%) short read pairs filtered out after trimming by size control 32544 ( 0.12%) empty read pairs filtered out after trimming by size control 28024134 (99.77%) read pairs available; of these: 22346573 (79.74%) trimmed read pairs available after processing 5677561 (20.26%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 19 4 0.00% 20 0 0.00% 21 2 0.00% 22 1 0.00% 23 1 0.00% 24 3 0.00% 25 5 0.00% 26 7 0.00% 27 5 0.00% 28 4 0.00% 29 6 0.00% 30 9 0.00% 31 4 0.00% 32 14 0.00% 33 10 0.00% 34 9 0.00% 35 16 0.00% 36 15 0.00% 37 15 0.00% 38 30 0.00% 39 28 0.00% 40 48 0.00% 41 35 0.00% 42 53 0.00% 43 66 0.00% 44 54 0.00% 45 62 0.00% 46 76 0.00% 47 109 0.00% 48 108 0.00% 49 113 0.00% 50 155 0.00% 51 166 0.00% 52 212 0.00% 53 207 0.00% 54 228 0.00% 55 279 0.00% 56 337 0.00% 57 401 0.00% 58 397 0.00% 59 483 0.00% 60 559 0.00% 61 646 0.00% 62 793 0.00% 63 914 0.00% 64 1078 0.00% 65 1187 0.00% 66 1316 0.00% 67 1457 0.01% 68 1694 0.01% 69 1886 0.01% 70 2243 0.01% 71 2562 0.01% 72 3077 0.01% 73 3435 0.01% 74 3513 0.01% 75 4517 0.02% 76 4260 0.02% 77 5056 0.02% 78 5222 0.02% 79 5705 0.02% 80 6372 0.02% 81 7162 0.03% 82 8348 0.03% 83 8578 0.03% 84 10035 0.04% 85 12514 0.04% 86 12811 0.05% 87 13824 0.05% 88 16043 0.06% 89 16774 0.06% 90 18570 0.07% 91 19002 0.07% 92 20215 0.07% 93 22464 0.08% 94 30593 0.11% 95 24420 0.09% 96 24879 0.09% 97 24142 0.09% 98 24637 0.09% 99 24400 0.09% 100 25396 0.09% 101 27019 0.10% 102 29573 0.11% 103 29372 0.10% 104 31839 0.11% 105 33785 0.12% 106 34455 0.12% 107 34770 0.12% 108 35061 0.13% 109 39387 0.14% 110 38318 0.14% 111 43222 0.15% 112 53118 0.19% 113 41182 0.15% 114 50822 0.18% 115 48155 0.17% 116 63574 0.23% 117 63461 0.23% 118 65137 0.23% 119 77282 0.28% 120 82714 0.30% 121 86068 0.31% 122 91822 0.33% 123 98519 0.35% 124 105081 0.37% 125 111942 0.40% 126 113505 0.41% 127 126693 0.45% 128 161469 0.58% 129 245688 0.88% 130 518239 1.85% 131 1120982 4.00% 132 820989 2.93% 133 4592252 16.39% 134 5895566 21.04% 135 65760 0.23% 136 48612 0.17% 137 50710 0.18% 138 63684 0.23% 139 102294 0.37% 140 552235 1.97% 141 1831078 6.53% 142 202764 0.72% 143 2338101 8.34% 144 102461 0.37% 145 713051 2.54% 146 34219 0.12% 147 40830 0.15% 148 53364 0.19% 149 84234 0.30% 150 626039 2.23% 151 5677561 20.26% 28024134 reads passed initial QC criterion=sequence-density sequence-density=0.37 sequence-density-rank=1 fanout-score=2.67 fanout-score-rank=20 prefix-density=0.40 prefix-fanout=2.4 sequence=TACTTGTTCAAC criterion=fanout-score sequence-density=0.01 sequence-density-rank=33 fanout-score=64.25 fanout-score-rank=1 prefix-density=0.20 prefix-fanout=4.6 sequence=ATAAAAAAAAGGGGGGGTAAGGACCCGCTAAGCTCCTACTTTTTCATGTTTCCAATCCGATCCCTCCGATTACTATAGAGATGAACCCAATCCAGAATATGAACCATAAAAGAAAACACCTACTAAACCAATCACAAGAATACCAGTTACCGTACCTATCAGCCAAAGAGGAATTCTTCCAGTAGTATCGGCCATTTCCCCTACTTTCCTCCACATTTTATCAAGTGGTCATGCTAGAGACAAAAACAGTCATGGATAGTTATGTTATAAGGATGGTATCCTTCCAAATGGGATAAGAGAGTTCTTACTACTCTCTTCTTTTCTCTCAATTAAAGAAGTAATTGGAAAACAAAACAGCAAGTACAAAAATGAGTAATAAACCCCAGTATAGACTGGTACGATTCAATTCAACATTTTGTTCATTCGGGTTTGATTGTGTCATAGTTCTATAGTTGGAATTTAGTTTATCGTTGGATGAACTGCATTGCTGATATTGATCCCAAGAAAAAAA criterion=sequence-density sequence-density=0.24 sequence-density-rank=1 fanout-score=1.99 fanout-score-rank=27 prefix-density=0.23 prefix-fanout=2.0 sequence=GTTGAACAAGTA criterion=fanout-score sequence-density=0.01 sequence-density-rank=33 fanout-score=52.76 fanout-score-rank=1 prefix-density=0.23 prefix-fanout=2.2 sequence=TTTTTTTTATTTTCTTAGACTTAGACCCTGCAAGATAATAATTTTTCGCTATTTACGATTTTATATTCTTGTTACTAGATACTCTATAGGAATAAGAATAAATCGCAACTCCTTTCCACTACACATAAAAATTGATTTCGAAAGTC SRR6941576 testing PE reads STAR mapping to Ensembl genome Started job on | Dec 06 11:54:22 Started mapping on | Dec 06 11:54:22 Finished on | Dec 06 11:57:19 Mapping speed, Million of reads per hour | 569.98 Number of input reads | 28024134 Average input read length | 275 UNIQUE READS: Uniquely mapped reads number | 17874672 Uniquely mapped reads % | 63.78% Average mapped length | 276.00 Number of splices: Total | 3448585 Number of splices: Annotated (sjdb) | 3124112 Number of splices: GT/AG | 3278470 Number of splices: GC/AG | 41278 Number of splices: AT/AC | 14581 Number of splices: Non-canonical | 114256 Mismatch rate per base, % | 0.35% Deletion rate per base | 0.01% Deletion average length | 1.48 Insertion rate per base | 0.01% Insertion average length | 2.01 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 8536257 % of reads mapped to multiple loci | 30.46% Number of reads mapped to too many loci | 14561 % of reads mapped to too many loci | 0.05% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 5.34% % of reads unmapped: other | 0.37% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 1635465 1635465 1635465 N_multimapping 8536257 8536257 8536257 N_noFeature 6859284 17159639 7195884 N_ambiguous 706222 8687 340684 UnstrandedReadsAssigned:10309166 PositiveStrandReadsAssigned:706346 NegativeStrandReadsAssigned:10338104 Dataset is classified negative stranded MeadianReadLen=151 20thPercentileLength=151 echo kmer=147 SRR6941576 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,972 [index] number of k-mers: 66,720,672 [index] number of equivalence classes: 111,837 [quant] running in paired-end mode [quant] will process pair 1: SRR6941576-trimmed-pair1.fastq SRR6941576-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 28,024,134 reads, 16,207,616 reads pseudoaligned [quant] estimated average fragment length: 241.074 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,023 rounds 52973 SRR6941576.ke.tsv 35125 SRR6941576.se.tsv 88098 total ==> SRR6941576.ke.tsv <== target_id length eff_length est_counts tpm PNS24245 936 696.402 0 0 PNS24247 1044 803.926 9.39073 0.740944 PNS24249 1928 1687.93 5.4236 0.203815 PNS24246 1044 803.926 9.39073 0.740944 PNS24248 1044 803.926 9.39073 0.740944 PNS24244 1471 1230.93 74.4042 3.83414 PNS24243 293 96.297 0 0 KQK14069 1603 1362.93 2214.13 103.047 KQK14071 474 243.805 42.8798 11.1561 ==> SRR6941576.se.tsv <== BRADI_1g14170v3 2995 BRADI_1g53295v3 22 BRADI_1g59795v3 119 BRADI_1g07683v3 0 BRADI_1g00485v3 0 BRADI_1g20270v3 81 BRADI_1g74790v3 17 BRADI_1g09890v3 0 BRADI_1g77505v3 84 BRADI_1g48960v3 0 SRR6941576 completed mapping pipeline successfully