Starting /dee2/code/volunteer_pipeline.sh SRR6941577 current disk space = 1551264837632 free memory = 1603735004 SRR6941577 SRAfilesize 7e04cdcee30d1bbc3d458e77ea6697a2 SRR6941577.sra SRR6941577.sra file validated SRR6941577 is paired end SRR6941577 is conventional basespace SRR6941577 read1 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR6941577_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 34.61425 35.0 35.0 35.0 35.0 35.0 2 34.6615 35.0 35.0 35.0 35.0 35.0 3 34.64 35.0 35.0 35.0 35.0 35.0 4 34.613 35.0 35.0 35.0 35.0 35.0 5 34.63625 35.0 35.0 35.0 35.0 35.0 6 39.4325 40.0 40.0 40.0 39.0 40.0 7 39.49225 40.0 40.0 40.0 39.0 40.0 8 39.28 40.0 40.0 40.0 39.0 40.0 9 39.42775 40.0 40.0 40.0 39.0 40.0 10-14 39.478300000000004 40.0 40.0 40.0 39.0 40.0 15-19 39.52714999999999 40.0 40.0 40.0 39.0 40.0 20-24 39.41715 40.0 40.0 40.0 39.0 40.0 25-29 39.42265 40.0 40.0 40.0 39.0 40.0 30-34 39.4217 40.0 40.0 40.0 39.0 40.0 35-39 39.40955 40.0 40.0 40.0 39.0 40.0 40-44 39.39485 40.0 40.0 40.0 39.0 40.0 45-49 39.39725 40.0 40.0 40.0 39.0 40.0 50-54 39.36725 40.0 40.0 40.0 39.0 40.0 55-59 39.36465 40.0 40.0 40.0 39.0 40.0 60-64 39.36175 40.0 40.0 40.0 39.0 40.0 65-69 39.346050000000005 40.0 40.0 40.0 39.0 40.0 70-74 39.3269 40.0 40.0 40.0 39.0 40.0 75-79 39.2742 40.0 40.0 40.0 38.8 40.0 80-84 39.2245 40.0 40.0 40.0 39.0 40.0 85-89 39.268600000000006 40.0 40.0 40.0 39.0 40.0 90-94 39.2715 40.0 40.0 40.0 39.0 40.0 95-99 39.14190000000001 40.0 39.8 40.0 38.6 40.0 100-104 38.384100000000004 39.4 38.6 39.6 37.0 39.8 105-109 39.1096 40.0 39.8 40.0 38.8 40.0 110-114 39.24655 40.0 40.0 40.0 39.0 40.0 115-119 39.282349999999994 40.0 40.0 40.0 39.0 40.0 120-124 39.206100000000006 40.0 40.0 40.0 39.0 40.0 125-129 39.087 40.0 40.0 40.0 38.4 40.0 130-134 39.0621 40.0 39.8 40.0 38.4 40.0 135-139 38.847300000000004 40.0 39.2 40.0 37.4 40.0 140-144 38.9491 40.0 39.4 40.0 37.8 40.0 145-149 38.831450000000004 40.0 39.2 40.0 37.6 40.0 150-151 37.499375 39.5 37.5 40.0 34.0 40.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 18 1.0 19 0.0 20 2.0 21 0.0 22 0.0 23 2.0 24 2.0 25 6.0 26 1.0 27 8.0 28 7.0 29 16.0 30 12.0 31 20.0 32 25.0 33 38.0 34 49.0 35 55.0 36 84.0 37 138.0 38 261.0 39 3273.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 43.583959899749374 10.37593984962406 6.817042606516291 39.22305764411028 2 22.45 13.475000000000001 32.675 31.4 3 22.025 17.599999999999998 24.474999999999998 35.9 4 24.775 26.1 21.349999999999998 27.775 5 25.825 31.3 23.0 19.875 6 19.25 37.7 22.650000000000002 20.4 7 12.3 33.75 38.975 14.975 8 17.549999999999997 26.625 33.35 22.475 9 17.424999999999997 24.15 36.325 22.1 10-14 18.26 34.515 25.85 21.375 15-19 19.55 31.785000000000004 25.895000000000003 22.770000000000003 20-24 17.164291072768194 31.337834458614655 28.29707426856714 23.20080020005001 25-29 21.235 31.22 27.655 19.89 30-34 21.645 32.975 24.375 21.005 35-39 20.575 31.415 27.26 20.75 40-44 18.973538092141464 31.354109349207143 26.48691911360112 23.185433445050272 45-49 19.035 29.82 29.225 21.92 50-54 20.419999999999998 31.385 26.43 21.765 55-59 20.27 30.354999999999997 25.885 23.49 60-64 18.75 31.545 27.025 22.68 65-69 19.605 32.36 25.674999999999997 22.36 70-74 20.255000000000003 30.740000000000002 24.79 24.215 75-79 20.74 29.925 27.134999999999998 22.2 80-84 21.29 30.56 25.480000000000004 22.67 85-89 22.185 29.970000000000002 26.064999999999998 21.78 90-94 18.955 31.540000000000003 26.674999999999997 22.830000000000002 95-99 20.05 32.629999999999995 24.135 23.185 100-104 20.135 32.515 25.035 22.314999999999998 105-109 19.86 31.019999999999996 25.990000000000002 23.13 110-114 20.895 30.159999999999997 25.480000000000004 23.465 115-119 20.115 32.005 24.64 23.24 120-124 20.445 31.314999999999998 23.494999999999997 24.745 125-129 20.405 32.33 23.87 23.395 130-134 21.865000000000002 32.105 23.61 22.42 135-139 20.8 31.540000000000003 24.83 22.830000000000002 140-144 23.095 30.615 24.895 21.395 145-149 20.525 31.795 24.265 23.415 150-151 20.674999999999997 32.337500000000006 23.1875 23.799999999999997 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1.0 1 0.5 2 0.0 3 0.5 4 0.5 5 0.0 6 0.0 7 0.5 8 0.5 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.5 16 0.5 17 0.5 18 0.5 19 0.5 20 2.5 21 3.0 22 4.5 23 6.5 24 6.0 25 6.5 26 12.5 27 10.5 28 8.0 29 17.0 30 27.5 31 35.0 32 35.0 33 37.5 34 47.0 35 58.5 36 96.0 37 204.0 38 289.5 39 294.5 40 306.5 41 316.0 42 275.0 43 258.0 44 235.5 45 194.0 46 170.5 47 147.0 48 123.0 49 90.5 50 72.0 51 58.5 52 50.5 53 48.5 54 51.0 55 71.0 56 73.0 57 45.0 58 36.0 59 37.0 60 29.5 61 22.5 62 20.0 63 20.5 64 16.0 65 8.5 66 5.5 67 2.5 68 2.0 69 1.5 70 1.5 71 2.5 72 1.0 73 0.0 74 0.0 75 0.5 76 0.5 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.25 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.025 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.045 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.975 #Duplication Level Percentage of deduplicated Percentage of total 1 83.35383353833538 50.824999999999996 2 7.708077080770807 9.4 3 3.034030340303403 5.55 4 1.5990159901599015 3.9 5 1.066010660106601 3.25 6 0.5330053300533005 1.95 7 0.4920049200492005 2.1 8 0.3280032800328003 1.6 9 0.2050020500205002 1.125 >10 1.5990159901599015 17.525 >50 0.08200082000820008 2.775 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GCGGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTACG 60 1.5 No Hit CTTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCAT 51 1.275 No Hit GTGCTCTGCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTCCTA 50 1.25 No Hit GGCCAAAATAACCATGAGCAGCCACAATATTATAAGTCTCTTCCTCTTGA 42 1.05 No Hit GCTTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCA 36 0.8999999999999999 No Hit GGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTACGTT 33 0.8250000000000001 No Hit CTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCATTAAT 24 0.6 No Hit CGCGACCTTGACTATCAACTACAGATTGGTTGAAATTGAATCCATTTAGG 23 0.575 No Hit GCGACCTTGACTATCAACTACAGATTGGTTGAAATTGAATCCATTTAGGT 22 0.5499999999999999 No Hit GTGAGCATTACGTTCGTGCATTACTTCCATACCAAGATTAGCACGGTTGA 21 0.525 No Hit CTCATAAGGACCACCATTGTATAACCATTCATCAACGGATGCAGCTTCCC 21 0.525 No Hit AGCGGAAAAACCTTATCCATTAATAGCGGGAACTTCAAGAGCAGCTAGAT 21 0.525 No Hit GCCAAAATAACCATGAGCAGCCACAATATTATAAGTCTCTTCCTCTTGAC 21 0.525 No Hit GGATGCATAAGGATGTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAA 20 0.5 No Hit CTCTGCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTCCTAAAG 20 0.5 No Hit CCACAATATTATAAGTCTCTTCCTCTTGACCAAATTTGTAACCCTCATTA 19 0.475 No Hit GCTCTGCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTCCTAAA 19 0.475 No Hit GTTGAAACTAGCATATTGGAAGATTAATCGGCCAAAATAACCATGAGCAG 18 0.44999999999999996 No Hit CGGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTACGT 17 0.42500000000000004 No Hit GTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTC 17 0.42500000000000004 No Hit CTCTTGACCAAATTTGTAACCCTCATTAGCAGATTCATTTTCAGTAGTTT 16 0.4 No Hit CCATGAGCAGCCACAATATTATAAGTCTCTTCCTCTTGACCAAATTTGTA 16 0.4 No Hit GTTAGCGGAAAAACCTTATCCATTAATAGCGGGAACTTCAAGAGCAGCTA 15 0.375 No Hit CTCATTAGCAGATTCATTTTCAGTAGTTTCCCTGATCAAACTAGAGGTTA 15 0.375 No Hit GGCGATAATAAATACAGAAGTTGCGGTCAATAAGGTAGGGATCATCAAAA 14 0.35000000000000003 No Hit CCACCATTGTATAACCATTCATCAACGGATGCAGCTTCCCAAATTGGGTA 14 0.35000000000000003 No Hit CCCAAGTATTAATAACGCGACCTTGACTATCAACTACAGATTGGTTGAAA 14 0.35000000000000003 No Hit GCTGCGATGAAGGCGATAATAAATACAGAAGTTGCGGTCAATAAGGTAGG 13 0.325 No Hit GGAGGGGCTGCGATGAAGGCGATAATAAATACAGAAGTTGCGGTCAATAA 12 0.3 No Hit GTTGAAATTGAATCCATTTAGGTTGAACGCCATAGTACTAATACCTAAAG 12 0.3 No Hit GCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTCCTAAAGGCAT 12 0.3 No Hit CCAGATTCCTACTACAGGCCAAGCAGCCAAGAAGAAGTGTAAAGAACGAG 11 0.27499999999999997 No Hit CATATAACAAGCTACACCAAGTAAGAAGTGTAGAACAATTAGCTCATAAG 11 0.27499999999999997 No Hit ACGCGACCTTGACTATCAACTACAGATTGGTTGAAATTGAATCCATTTAG 11 0.27499999999999997 No Hit GGGGCTGCGATGAAGGCGATAATAAATACAGAAGTTGCGGTCAATAAGGT 11 0.27499999999999997 No Hit GCACGGTTGATGATATCAGCCCAAGTATTAATAACGCGACCTTGACTATC 10 0.25 No Hit CGCAGAAGTAGGAATAATAGCACCAGAGATAATATTGTTTCCATAAAGTA 10 0.25 No Hit CCGCAGAAGTAGGAATAATAGCACCAGAGATAATATTGTTTCCATAAAGT 10 0.25 No Hit TTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCATT 10 0.25 No Hit CGGAAAAACCTTATCCATTAATAGCGGGAACTTCAAGAGCAGCTAGATCT 10 0.25 No Hit GGCCAAGCAGCCAAGAAGAAGTGTAAAGAACGAGAGTTGTTGAAACTAGC 10 0.25 No Hit CGCCATAGTACTAATACCTAAAGCAGTGAACCAGATTCCTACTACAGGCC 9 0.22499999999999998 No Hit GCCGAAAACACCAGCTACACCTAACATGTGAAATGGATGCATAAGGATGT 9 0.22499999999999998 No Hit GTTGATGATATCAGCCCAAGTATTAATAACGCGACCTTGACTATCAACTA 9 0.22499999999999998 No Hit CCACTCACGACCCATATAACAAGCTACACCAAGTAAGAAGTGTAGAACAA 9 0.22499999999999998 No Hit GCGGAAAAACCTTATCCATTAATAGCGGGAACTTCAAGAGCAGCTAGATC 9 0.22499999999999998 No Hit ACCAGATATTCCTAAAGGCATACCATCAGAGAAGCTTCCTTGACCAATAG 8 0.2 No Hit CAAGTATTAATAACGCGACCTTGACTATCAACTACAGATTGGTTGAAATT 8 0.2 No Hit CCCTCATTAGCAGATTCATTTTCAGTAGTTTCCCTGATCAAACTAGAGGT 8 0.2 No Hit ATCCAGTTGCAGAAGCGACCCCACAGGCTTGTACTTTCGCGTCTCTCTAA 8 0.2 No Hit GATGCATAAGGATGTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAAA 8 0.2 No Hit GTCCATGTACCAGTAGAAGATTCGGCAGCTACTGCAGCCCCTGCTTCTTC 8 0.2 No Hit GGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTACGTTC 8 0.2 No Hit GCCAAGAAGAAGTGTAAAGAACGAGAGTTGTTGAAACTAGCATATTGGAA 8 0.2 No Hit CTGCGATGAAGGCGATAATAAATACAGAAGTTGCGGTCAATAAGGTAGGG 7 0.17500000000000002 No Hit CAGTGAACCAGATTCCTACTACAGGCCAAGCAGCCAAGAAGAAGTGTAAA 7 0.17500000000000002 No Hit AGGAATAATAGCACCAGAGATAATATTGTTTCCATAAAGTAAAGAACCAG 7 0.17500000000000002 No Hit GTAGGGATCATCAAAACACCGAACCATCCGATGTAAAGACGATTTTCAGT 7 0.17500000000000002 No Hit GCCACAATATTATAAGTCTCTTCCTCTTGACCAAATTTGTAACCCTCATT 7 0.17500000000000002 No Hit CCAAGTATTAATAACGCGACCTTGACTATCAACTACAGATTGGTTGAAAT 7 0.17500000000000002 No Hit GCATAAGGATGTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAAAGTA 7 0.17500000000000002 No Hit CCCAGACATACGCAATGCTTTAGCTAATACACGGAAATGCATACCATGAT 7 0.17500000000000002 No Hit CACCATTGTATAACCATTCATCAACGGATGCAGCTTCCCAAATTGGGTAA 7 0.17500000000000002 No Hit GTCAATAAGGTAGGGATCATCAAAACACCGAACCATCCGATGTAAAGACG 7 0.17500000000000002 No Hit CCCCACTGCTGCCTCCCGTAGGAGTCTGGGCCGTGTCTCAGTCCCAGTGT 7 0.17500000000000002 No Hit CTCGCGAATACCATCAATATCTACTGGAGGGGCTGCGATGAAGGCGATAA 7 0.17500000000000002 No Hit GCGGTCAATAAGGTAGGGATCATCAAAACACCGAACCATCCGATGTAAAG 6 0.15 No Hit GCGATAATAAATACAGAAGTTGCGGTCAATAAGGTAGGGATCATCAAAAC 6 0.15 No Hit CTTGACTATCAACTACAGATTGGTTGAAATTGAATCCATTTAGGTTGAAC 6 0.15 No Hit GGTTGATGATATCAGCCCAAGTATTAATAACGCGACCTTGACTATCAACT 6 0.15 No Hit GGGAAGTTGTGAGCATTACGTTCGTGCATTACTTCCATACCAAGATTAGC 6 0.15 No Hit ATCGGCCAAAATAACCATGAGCAGCCACAATATTATAAGTCTCTTCCTCT 6 0.15 No Hit ATAAGGATGTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAAAGTACC 6 0.15 No Hit CCCACTGCTGCCTCCCGTAGGAGTCTGGGCCGTGTCTCAGTCCCAGTGTG 6 0.15 No Hit CTTATATGTTAGCGGAAAAACCTTATCCATTAATAGCGGGAACTTCAAGA 6 0.15 No Hit CTCCACGCGGCATTGCTCCGTCAGGCTTTCGCCCATTGCGGAAAATTCCC 6 0.15 No Hit AGCAGCCACAATATTATAAGTCTCTTCCTCTTGACCAAATTTGTAACCCT 6 0.15 No Hit GACCCATATAACAAGCTACACCAAGTAAGAAGTGTAGAACAATTAGCTCA 6 0.15 No Hit CACCTAACATGTGAAATGGATGCATAAGGATGTTGTGCTCTGCCTGGAAT 6 0.15 No Hit CTTCCTCTTGACCAAATTTGTAACCCTCATTAGCAGATTCATTTTCAGTA 5 0.125 No Hit CAGAGAAGCTTCCTTGACCAATAGGGTAAATCAAGAAAACAGCAGTCGCA 5 0.125 No Hit ACGAGAGTTGTTGAAACTAGCATATTGGAAGATTAATCGGCCAAAATAAC 5 0.125 No Hit GGCGGAAAAACCTTATCCATTAATAGCGGGAACTTCAAGAGCAGCTAGAT 5 0.125 No Hit CGAGAGTTGTTGAAACTAGCATATTGGAAGATTAATCGGCCAAAATAACC 5 0.125 No Hit GGCATGATGACTTGGCCTCATCCTCTCCTTCCTCCGGCTTAACACCGGCG 5 0.125 No Hit GTCTCTTCCTCTTGACCAAATTTGTAACCCTCATTAGCAGATTCATTTTC 5 0.125 No Hit GCATTACGTTCGTGCATTACTTCCATACCAAGATTAGCACGGTTGATGAT 5 0.125 No Hit TTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCATTA 5 0.125 No Hit GATGATATCAGCCCAAGTATTAATAACGCGACCTTGACTATCAACTACAG 5 0.125 No Hit GCATATTGGAAGATTAATCGGCCAAAATAACCATGAGCAGCCACAATATT 5 0.125 No Hit GGTCAATAAGGTAGGGATCATCAAAACACCGAACCATCCGATGTAAAGAC 5 0.125 No Hit AGCATATTGGAAGATTAATCGGCCAAAATAACCATGAGCAGCCACAATAT 5 0.125 No Hit CTCGCTTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTAT 5 0.125 No Hit CTCGAGAACAGCTAAACCTACATTACATGCAATAGTACCTAGAATTACTA 5 0.125 No Hit CAGAGATAATATTGTTTCCATAAAGTAAAGAACCAGAAACAGGCTCGCGA 5 0.125 No Hit ATCAATATCTACTGGAGGGGCTGCGATGAAGGCGATAATAAATACAGAAG 5 0.125 No Hit CCTCCACCAAATTGTAATACAGAATCATCCCCAAAGATTTCGGTCAGAGC 5 0.125 No Hit CCTAGCTTTCGTCTCTCAGTGTCAGTGTCGGCCCAGCAGAGTGCTTTCGC 5 0.125 No Hit TAAAGAACGAGAGTTGTTGAAACTAGCATATTGGAAGATTAATCGGCCAA 5 0.125 No Hit CGGTCAATAAGGTAGGGATCATCAAAACACCGAACCATCCGATGTAAAGA 5 0.125 No Hit GGCGGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTAC 5 0.125 No Hit CCTCATTAGCAGATTCATTTTCAGTAGTTTCCCTGATCAAACTAGAGGTT 5 0.125 No Hit CATAAGGACCACCATTGTATAACCATTCATCAACGGATGCAGCTTCCCAA 5 0.125 No Hit GAGCATTACGTTCGTGCATTACTTCCATACCAAGATTAGCACGGTTGATG 5 0.125 No Hit CGCCGAAAACACCAGCTACACCTAACATGTGAAATGGATGCATAAGGATG 5 0.125 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0125 0.0 0.0 0.0 0.0 62-63 0.025 0.0 0.0 0.0 0.0 64-65 0.025 0.0 0.0 0.0 0.0 66-67 0.025 0.0 0.0 0.0 0.0 68-69 0.025 0.0 0.0 0.0 0.0 70-71 0.025 0.0 0.0 0.0 0.0 72-73 0.0625 0.0 0.0 0.0 0.0 74-75 0.125 0.0 0.0 0.0 0.0 76-77 0.1375 0.0 0.0 0.0 0.0 78-79 0.175 0.0 0.0 0.0 0.0 80-81 0.2625 0.0 0.0 0.0 0.0 82-83 0.35 0.0 0.0 0.0 0.0 84-85 0.425 0.0 0.0 0.0 0.0 86-87 0.5625 0.0 0.0 0.0 0.0 88-89 0.7250000000000001 0.0 0.0 0.0 0.0 90-91 0.875 0.0 0.0 0.0 0.0 92-93 0.9375 0.0 0.0 0.0 0.0 94-95 1.15 0.0 0.0 0.0 0.0 96-97 1.325 0.0 0.0 0.0 0.0 98-99 1.5375 0.0 0.0 0.0 0.0 100-101 1.6625 0.0 0.0 0.0 0.0 102-103 1.8375 0.0 0.0 0.0 0.0 104-105 2.0 0.0 0.0 0.0 0.0 106-107 2.35 0.0 0.0 0.0 0.0 108-109 2.725 0.0 0.0 0.0 0.0 110-111 3.05 0.0 0.0 0.0 0.0 112-113 3.4625 0.0 0.0 0.0 0.0 114-115 3.8375000000000004 0.0 0.0 0.0 0.0 116-117 4.275 0.0 0.0 0.0 0.0 118-119 4.637499999999999 0.0 0.0 0.0 0.0 120-121 4.887499999999999 0.0 0.0 0.0 0.0 122-123 5.1625 0.0 0.0 0.0 0.0 124-125 5.612500000000001 0.0 0.0 0.0 0.0 126-127 6.025 0.0 0.0 0.0 0.0 128-129 6.324999999999999 0.0 0.0 0.0 0.0 130-131 6.7875 0.0 0.0 0.0 0.0 132-133 7.225 0.0 0.0 0.0 0.0 134-135 7.7 0.0 0.0 0.0 0.0 136-137 8.1125 0.0 0.0 0.0 0.0 138-139 8.5125 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCCAAAG 10 0.0068555363 144.825 1 TTAAGGA 10 0.0068555363 144.825 8 GATTTCG 10 0.0068555363 144.825 7 AGATTTC 10 0.0068555363 144.825 6 ATTTCGG 10 0.0068555363 144.825 8 GCGGGAA 35 3.053392E-5 82.75714 1 CGGGAAC 35 3.053392E-5 82.75714 2 GGGAACT 40 5.9210775E-5 72.4125 3 ACTTCAA 45 1.06126114E-4 64.36667 7 GGAACTT 45 1.06126114E-4 64.36667 4 AAATTGA 35 0.0033283124 62.067856 145 CTTCAAG 50 1.7875935E-4 57.93 8 AACTTCA 50 1.7875935E-4 57.93 6 GAACTTC 50 1.7875935E-4 57.93 5 TTCAAGA 55 2.863462E-4 52.663635 9 AGAGCAG 40 2.9323745E-4 21.778196 10-14 ACAGATT 35 0.003561457 20.689285 130-134 GAAATTG 35 0.003561457 20.689285 140-144 CAACTAC 35 0.003561457 20.689285 125-129 AACTACA 35 0.003561457 20.689285 125-129 >>END_MODULE SRR6941577 read2 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR6941577_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 34.00775 35.0 35.0 35.0 33.0 35.0 2 33.9735 35.0 35.0 35.0 32.0 35.0 3 33.8455 35.0 35.0 35.0 31.0 35.0 4 34.15175 35.0 35.0 35.0 33.0 35.0 5 34.1845 35.0 35.0 35.0 33.0 35.0 6 38.73975 40.0 40.0 40.0 37.0 40.0 7 39.01175 40.0 40.0 40.0 38.0 40.0 8 39.00225 40.0 40.0 40.0 38.0 40.0 9 39.032 40.0 40.0 40.0 39.0 40.0 10-14 38.879650000000005 40.0 40.0 40.0 38.4 40.0 15-19 38.6655 40.0 39.8 40.0 37.2 40.0 20-24 38.9782 40.0 40.0 40.0 38.4 40.0 25-29 38.954550000000005 40.0 40.0 40.0 38.4 40.0 30-34 38.916650000000004 40.0 40.0 40.0 38.0 40.0 35-39 38.86514999999999 40.0 40.0 40.0 38.0 40.0 40-44 38.987350000000006 40.0 40.0 40.0 38.6 40.0 45-49 38.741 40.0 39.8 40.0 37.2 40.0 50-54 38.88075 40.0 40.0 40.0 37.6 40.0 55-59 38.812400000000004 40.0 39.8 40.0 37.6 40.0 60-64 38.92954999999999 40.0 40.0 40.0 38.2 40.0 65-69 38.79915 40.0 39.8 40.0 37.6 40.0 70-74 38.549850000000006 40.0 39.2 40.0 36.4 40.0 75-79 38.7636 40.0 39.8 40.0 37.6 40.0 80-84 38.84375 40.0 39.8 40.0 37.8 40.0 85-89 38.5432 40.0 39.0 40.0 36.4 40.0 90-94 38.5219 40.0 39.0 40.0 36.0 40.0 95-99 38.3571 40.0 39.0 40.0 35.6 40.0 100-104 37.20745000000001 38.8 37.8 39.4 33.6 39.6 105-109 38.144600000000004 40.0 39.0 40.0 35.4 40.0 110-114 38.0663 40.0 39.0 40.0 35.6 40.0 115-119 37.86835 40.0 39.0 40.0 34.8 40.0 120-124 37.851 40.0 39.0 40.0 34.6 40.0 125-129 37.5278 40.0 38.8 40.0 33.4 40.0 130-134 37.90955 40.0 39.0 40.0 34.8 40.0 135-139 37.6935 40.0 39.0 40.0 34.6 40.0 140-144 37.53245 40.0 39.0 40.0 34.0 40.0 145-149 36.813750000000006 40.0 38.8 40.0 31.8 40.0 150-151 34.835 38.5 35.0 39.5 25.0 40.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 7.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 2.0 10 0.0 11 0.0 12 0.0 13 0.0 14 1.0 15 1.0 16 1.0 17 2.0 18 3.0 19 2.0 20 12.0 21 7.0 22 9.0 23 12.0 24 13.0 25 18.0 26 20.0 27 25.0 28 31.0 29 29.0 30 31.0 31 35.0 32 30.0 33 46.0 34 62.0 35 85.0 36 110.0 37 178.0 38 363.0 39 2865.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.05545752457776 17.796823796319636 13.410637761532646 28.73708091756995 2 25.270304249434243 23.05758109127483 36.20819713351773 15.463917525773196 3 19.228843264897346 25.137706559839764 39.48422633950926 16.14922383575363 4 24.660291897332662 30.724710619023654 26.220432813286358 18.394564670357322 5 24.191526698420656 31.260967661067934 28.102281273502133 16.445224367009274 6 20.43172690763052 36.04417670682731 26.8574297188755 16.666666666666664 7 16.478555304740404 21.369450714823177 44.16854778028593 17.98344620015049 8 21.061858251940897 24.61808164287503 32.88254445279239 21.437515652391685 9 22.113698973203107 20.485850237916353 35.58727773603806 21.813173052842476 10-14 22.79927812312011 26.178062963705635 32.11349508722679 18.909163825947463 15-19 22.802528597230584 24.959863536022475 32.39514348785872 19.84246437888822 20-24 23.39679358717435 25.83166332665331 32.06412825651302 18.70741482965932 25-29 22.62590829366074 25.512402906539716 31.93184665497369 19.92984214482586 30-34 23.925673645196834 25.112691575678653 32.13462886907743 18.827005910047077 35-39 23.77755511022044 25.180360721442884 31.683366733466933 19.35871743486974 40-44 22.914265671193064 25.1190058626046 31.42255850077667 20.544169965425667 45-49 22.359719438877757 26.452905811623246 31.713426853707418 19.473947895791582 50-54 22.569653237121667 25.72659851673682 31.814992984566043 19.888755261575465 55-59 22.374749498997996 27.059118236472944 30.66633266533066 19.8997995991984 60-64 22.53168361468717 25.341882482592798 31.868957571507288 20.257476331212743 65-69 22.993688007213706 25.283037771766352 31.569982967638516 20.153291253381425 70-74 22.968640416791906 25.593627892996697 31.585011521891595 19.852720168319806 75-79 23.44658248145921 25.125275606333936 31.343956704750454 20.084185207456404 80-84 23.41331463206933 24.680659219556176 33.26153383759956 18.644492310774936 85-89 23.70266479663394 25.826487677820076 30.925666199158485 19.5451813263875 90-94 23.295426080857673 24.93362056009218 31.06557787685988 20.705375482190274 95-99 23.11082177374931 25.18904301667585 31.353598077019377 20.34653713255546 100-104 23.516715189238028 25.569721915470332 31.362313020781045 19.55124987451059 105-109 24.56378538743903 24.2268818826369 31.306883894001107 19.902448835922964 110-114 23.407146451937592 24.85153497735279 31.545042778057375 20.19627579265224 115-119 23.978489219480323 25.189727094536863 31.41679650198522 19.414987183997585 120-124 23.188916876574307 26.045340050377835 29.90931989924433 20.856423173803528 125-129 24.565719449743952 25.62506275730495 30.0632593633899 19.7459584295612 130-134 23.70563989142455 25.67105659997989 30.83844375188499 19.784859756710567 135-139 24.06829170024174 25.700040290088637 30.89746172441579 19.334206285253828 140-144 24.52640064490125 26.063079403466343 30.26501410721483 19.145505844417574 145-149 24.19103215741533 25.177394192541897 30.647677520004024 19.98389613003875 150-151 23.420027641663527 24.739288855383844 32.56690539012439 19.273778112828243 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 7.0 1 3.5 2 0.0 3 1.0 4 1.5 5 0.5 6 1.0 7 1.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.5 19 1.0 20 0.5 21 1.0 22 4.0 23 5.5 24 7.0 25 10.5 26 15.0 27 18.5 28 20.0 29 25.5 30 28.5 31 38.5 32 43.0 33 53.5 34 86.0 35 97.5 36 105.5 37 178.0 38 236.5 39 232.5 40 273.5 41 288.5 42 235.0 43 240.5 44 266.5 45 231.0 46 194.0 47 164.5 48 122.0 49 85.5 50 64.5 51 63.5 52 56.5 53 51.5 54 49.5 55 59.0 56 66.5 57 43.5 58 34.0 59 35.5 60 32.0 61 30.5 62 30.0 63 22.5 64 12.5 65 7.5 66 5.5 67 4.0 68 1.5 69 1.5 70 2.0 71 2.0 72 1.0 73 0.0 74 0.0 75 0.0 76 0.5 77 0.5 78 0.0 79 0.5 80 0.5 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.8250000000000001 2 0.575 3 0.15 4 0.65 5 0.27499999999999997 6 0.4 7 0.325 8 0.17500000000000002 9 0.17500000000000002 10-14 0.26 15-19 0.33999999999999997 20-24 0.2 25-29 0.22499999999999998 30-34 0.16999999999999998 35-39 0.2 40-44 0.215 45-49 0.2 50-54 0.22 55-59 0.2 60-64 0.185 65-69 0.19 70-74 0.19 75-79 0.22 80-84 0.185 85-89 0.18 90-94 0.19499999999999998 95-99 0.155 100-104 0.38999999999999996 105-109 0.565 110-114 0.65 115-119 0.515 120-124 0.75 125-129 0.41000000000000003 130-134 0.53 135-139 0.72 140-144 0.76 145-149 0.645 150-151 0.5125000000000001 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 64.64999999999999 #Duplication Level Percentage of deduplicated Percentage of total 1 81.86388244392884 52.925 2 8.313998453209589 10.75 3 3.5189481825212683 6.825 4 1.9334880123743232 5.0 5 1.1214230471771076 3.6249999999999996 6 0.6573859242072699 2.55 7 0.42536736272235115 1.925 8 0.502706883217324 2.6 9 0.46403712296983757 2.7 >10 1.1987625676720803 11.1 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source ATTATCTATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAAT 34 0.8500000000000001 No Hit AGGGAAACTACTGAAAATGAATCTGCTAATGAGGGTTACAAATTTGGTCA 21 0.525 No Hit GCTAGTTTCAACAACTCTCGTTCTTTACACTTCTTCTTGGCTGCTTGGCC 20 0.5 No Hit TATCTATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTT 19 0.475 No Hit GCTTGTTATATGGGTCGTGAGTGGGAACTTAGTTTCCGTTTGGGTATGCG 18 0.44999999999999996 No Hit GTGGGAACTTAGTTTCCGTTTGGGTATGCGCCCTTGGATTGCTGTTGCAT 16 0.4 No Hit GTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGATCTTACCATGAC 16 0.4 No Hit GGAAGAGACTTATAATATTGTGGCTGCTCATGGTTATTTTGGCCGATTAA 15 0.375 No Hit CTGGTACTTTCAACTTTATGATTGTATTCCAGGCAGAGCACAACATCCTT 15 0.375 No Hit GGAAGCTTCTCTGATGGTATGCCTTTAGGAATATCTGGTACTTTCAACTT 15 0.375 No Hit CTATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAA 14 0.35000000000000003 No Hit GTTATTTTGGCCGATTAATCTTCCAATATGCTAGTTTCAACAACTCTCGT 14 0.35000000000000003 No Hit GTTGCATATTCAGCTCCTGTTGCAGCTGCGACTGCTGTTTTCTTGATTTA 14 0.35000000000000003 No Hit GCACAACATCCTTATGCATCCATTTCACATGTTAGGTGTAGCTGGTGTTT 14 0.35000000000000003 No Hit GGGAAACTACTGAAAATGAATCTGCTAATGAGGGTTACAAATTTGGTCAA 13 0.325 No Hit GCATGGTTCCTTGGTAACCTCTAGTTTGATCAGGGAAACTACTGAAAATG 13 0.325 No Hit GTTCGATCCTGGCTCAGGATGAACGCTGGCGGCATGCTTAACACATGCAA 13 0.325 No Hit GTTCAACCTAAATGGATTCAATTTCAACCAATCTGTAGTTGATAGTCAAG 13 0.325 No Hit CTTCCAATATGCTAGTTTCAACAACTCTCGTTCTTTACACTTCTTCTTGG 13 0.325 No Hit CTGAAAATGAATCTGCTAATGAGGGTTACAAATTTGGTCAAGAGGAAGAG 13 0.325 No Hit GTTATACAATGGTGGTCCTTATGAGCTAATTGTTCTACACTTCTTACTTG 12 0.3 No Hit CTTAGTTTCCGTTTGGGTATGCGCCCTTGGATTGCTGTTGCATATTCAGC 12 0.3 No Hit GTAGGAATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCT 12 0.3 No Hit TCTATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGA 12 0.3 No Hit GTTTGATCAGGGAAACTACTGAAAATGAATCTGCTAATGAGGGTTACAAA 12 0.3 No Hit GTTTCAACAACTCTCGTTCTTTACACTTCTTCTTGGCTGCTTGGCCTGTA 11 0.27499999999999997 No Hit AGCACAACATCCTTATGCATCCATTTCACATGTTAGGTGTAGCTGGTGTT 10 0.25 No Hit ATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAA 10 0.25 No Hit GTTTTGATGATCCCTACCTTATTGACCGCAACTTCTGTATTTATTATCGC 10 0.25 No Hit CTAGCACTGAAAATCGTCTTTACATCGGATGGTTCGGTGTTTTGATGATC 10 0.25 No Hit GAAGAGACTTATAATATTGTGGCTGCTCATGGTTATTTTGGCCGATTAAT 10 0.25 No Hit GCTAATTGTTCTACACTTCTTACTTGGTGTAGCTTGTTATATGGGTCGTG 9 0.22499999999999998 No Hit ATCGGATTGCACTTTTACCCAATTTGGGAAGCTGCATCCGTTGATGAATG 9 0.22499999999999998 No Hit GGGTTACAAATTTGGTCAAGAGGAAGAGACTTATAATATTGTGGCTGCTC 9 0.22499999999999998 No Hit CTTGCAATTTGAATAAACCAAGATCTTACCATGACTGCAATTTTAGAGAG 9 0.22499999999999998 No Hit AAACAATATTATCTCTGGTGCTATTATTCCTACTTCTGCGGCAATCGGAT 9 0.22499999999999998 No Hit TATGCCTTTAGGAATATCTGGTACTTTCAACTTTATGATTGTATTCCAGG 9 0.22499999999999998 No Hit CAATGGTGGTCCTTATGAGCTAATTGTTCTACACTTCTTACTTGGTGTAG 9 0.22499999999999998 No Hit CGAAAGTACAAGCCTGTGGGGTCGCTTCTGCAACTGGATAACTAGCACTG 9 0.22499999999999998 No Hit GCTGCGACTGCTGTTTTCTTGATTTACCCTATTGGTCAAGGAAGCTTCTC 9 0.22499999999999998 No Hit GTTAGGTGTAGCTGGTGTTTTCGGCGGTTCCCTATTCAGTGCTATGCATG 9 0.22499999999999998 No Hit GAATGGTTATACAATGGTGGTCCTTATGAGCTAATTGTTCTACACTTCTT 9 0.22499999999999998 No Hit ATCTATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTG 9 0.22499999999999998 No Hit GTTTTCTTGATTTACCCTATTGGTCAAGGAAGCTTCTCTGATGGTATGCC 8 0.2 No Hit GTCGTGAGTGGGAACTTAGTTTCCGTTTGGGTATGCGCCCTTGGATTGCT 8 0.2 No Hit CAACTCTCGTTCTTTACACTTCTTCTTGGCTGCTTGGCCTGTAGTAGGAA 8 0.2 No Hit CCTAAATGGATTCAATTTCAACCAATCTGTAGTTGATAGTCAAGGTCGCG 8 0.2 No Hit ATCGCCTTCATCGCAGCCCCTCCAGTAGATATTGATGGTATTCGCGAGCC 8 0.2 No Hit ATTTGGTCAAGAGGAAGAGACTTATAATATTGTGGCTGCTCATGGTTATT 8 0.2 No Hit GTTGATAGTCAAGGTCGCGTTATTAATACTTGGGCTGATATCATCAACCG 8 0.2 No Hit GGAATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCTAAA 8 0.2 No Hit GGCTTGCGGTGGATACCTAGGCACCCAGAGACGAGGAAGGGCGTAGCAAG 8 0.2 No Hit CTTACCATGACTGCAATTTTAGAGAGACGCGAAAGTACAAGCCTGTGGGG 8 0.2 No Hit GAGGAAGAGACTTATAATATTGTGGCTGCTCATGGTTATTTTGGCCGATT 8 0.2 No Hit CGGATGGTTCGGTGTTTTGATGATCCCTACCTTATTGACCGCAACTTCTG 8 0.2 No Hit CAACATCCTTATGCATCCATTTCACATGTTAGGTGTAGCTGGTGTTTTCG 8 0.2 No Hit GATTAATCTTCCAATATGCTAGTTTCAACAACTCTCGTTCTTTACACTTC 7 0.17500000000000002 No Hit TGGGAACTTAGTTTCCGTTTGGGTATGCGCCCTTGGATTGCTGTTGCATA 7 0.17500000000000002 No Hit TTATAATATTGTGGCTGCTCATGGTTATTTTGGCCGATTAATCTTCCAAT 7 0.17500000000000002 No Hit GAACTTAGTTTCCGTTTGGGTATGCGCCCTTGGATTGCTGTTGCATATTC 7 0.17500000000000002 No Hit CTTGTTATATGGGTCGTGAGTGGGAACTTAGTTTCCGTTTGGGTATGCGC 7 0.17500000000000002 No Hit GTGCTATGCATGGTTCCTTGGTAACCTCTAGTTTGATCAGGGAAACTACT 7 0.17500000000000002 No Hit GCCTTTAGGAATATCTGGTACTTTCAACTTTATGATTGTATTCCAGGCAG 7 0.17500000000000002 No Hit TGTAGCTGGTGTTTTCGGCGGTTCCCTATTCAGTGCTATGCATGGTTCCT 7 0.17500000000000002 No Hit GTCAAGGTCGCGTTATTAATACTTGGGCTGATATCATCAACCGTGCTAAT 7 0.17500000000000002 No Hit CTCATGGTTATTTTGGCCGATTAATCTTCCAATATGCTAGTTTCAACAAC 7 0.17500000000000002 No Hit GGTACTTTCAACTTTATGATTGTATTCCAGGCAGAGCACAACATCCTTAT 7 0.17500000000000002 No Hit CCTTGTTATTGTGAGAATTCTTAATTCAAGAGTTGTAAGGAGGGACTTAT 6 0.15 No Hit CATGGTTATTTTGGCCGATTAATCTTCCAATATGCTAGTTTCAACAACTC 6 0.15 No Hit GGTAACCTCTAGTTTGATCAGGGAAACTACTGAAAATGAATCTGCTAATG 6 0.15 No Hit TATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAA 6 0.15 No Hit CCAAGATCTTACCATGACTGCAATTTTAGAGAGACGCGAAAGTACAAGCC 6 0.15 No Hit CTTATGAGCTAATTGTTCTACACTTCTTACTTGGTGTAGCTTGTTATATG 6 0.15 No Hit CGTTGATGAATGGTTATACAATGGTGGTCCTTATGAGCTAATTGTTCTAC 6 0.15 No Hit GACTTATAATATTGTGGCTGCTCATGGTTATTTTGGCCGATTAATCTTCC 6 0.15 No Hit GTTATATGGGTCGTGAGTGGGAACTTAGTTTCCGTTTGGGTATGCGCCCT 6 0.15 No Hit CTGGTGTTTTCGGCGGTTCCCTATTCAGTGCTATGCATGGTTCCTTGGTA 6 0.15 No Hit CAAATTTGGTCAAGAGGAAGAGACTTATAATATTGTGGCTGCTCATGGTT 6 0.15 No Hit ATTCAGTGCTATGCATGGTTCCTTGGTAACCTCTAGTTTGATCAGGGAAA 6 0.15 No Hit ATTGTATTCCAGGCAGAGCACAACATCCTTATGCATCCATTTCACATGTT 6 0.15 No Hit GCTGCATCCGTTGATGAATGGTTATACAATGGTGGTCCTTATGAGCTAAT 6 0.15 No Hit GATTTACCCTATTGGTCAAGGAAGCTTCTCTGATGGTATGCCTTTAGGAA 6 0.15 No Hit GTTCTTTACTTTATGGAAACAATATTATCTCTGGTGCTATTATTCCTACT 6 0.15 No Hit GTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGATCTTACCATGACTG 6 0.15 No Hit ATTATTCCTACTTCTGCGGCAATCGGATTGCACTTTTACCCAATTTGGGA 5 0.125 No Hit CCGCAACTTCTGTATTTATTATCGCCTTCATCGCAGCCCCTCCAGTAGAT 5 0.125 No Hit GTGGCTGCTCATGGTTATTTTGGCCGATTAATCTTCCAATATGCTAGTTT 5 0.125 No Hit GGAAACTACTGAAAATGAATCTGCTAATGAGGGTTACAAATTTGGTCAAG 5 0.125 No Hit CGTCTTTACATCGGATGGTTCGGTGTTTTGATGATCCCTACCTTATTGAC 5 0.125 No Hit CTTCTGTATTTATTATCGCCTTCATCGCAGCCCCTCCAGTAGATATTGAT 5 0.125 No Hit TATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAAT 5 0.125 No Hit GAGACTTATAATATTGTGGCTGCTCATGGTTATTTTGGCCGATTAATCTT 5 0.125 No Hit CTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCTAAATGGAT 5 0.125 No Hit CTTCTTCTTGGCTGCTTGGCCTGTAGTAGGAATCTGGTTCACTGCTTTAG 5 0.125 No Hit GGAATATCTGGTACTTTCAACTTTATGATTGTATTCCAGGCAGAGCACAA 5 0.125 No Hit ATAATATTGTGGCTGCTCATGGTTATTTTGGCCGATTAATCTTCCAATAT 5 0.125 No Hit CGCAGCCCCTCCAGTAGATATTGATGGTATTCGCGAGCCTGTTTCTGGTT 5 0.125 No Hit GTTACAAATTTGGTCAAGAGGAAGAGACTTATAATATTGTGGCTGCTCAT 5 0.125 No Hit CCTATTCAGTGCTATGCATGGTTCCTTGGTAACCTCTAGTTTGATCAGGG 5 0.125 No Hit GTAGCTTGTTATATGGGTCGTGAGTGGGAACTTAGTTTCCGTTTGGGTAT 5 0.125 No Hit TATACAATGGTGGTCCTTATGAGCTAATTGTTCTACACTTCTTACTTGGT 5 0.125 No Hit AGGAAGCTTCTCTGATGGTATGCCTTTAGGAATATCTGGTACTTTCAACT 5 0.125 No Hit CTTGATTTACCCTATTGGTCAAGGAAGCTTCTCTGATGGTATGCCTTTAG 5 0.125 No Hit GTTATTGTGAGAATTCTTAATTCAAGAGTTGTAAGGAGGGACTTATGTCA 5 0.125 No Hit CATCAACCGTGCTAATCTTGGTATGGAAGTAATGCACGAACGTAATGCTC 5 0.125 No Hit GTGAGAATTCTTAATTCAAGAGTTGTAAGGAGGGACTTATGTCACCACAA 5 0.125 No Hit GTTTCTGGTTCTTTACTTTATGGAAACAATATTATCTCTGGTGCTATTAT 5 0.125 No Hit CACATGTTAGGTGTAGCTGGTGTTTTCGGCGGTTCCCTATTCAGTGCTAT 5 0.125 No Hit CAGGGAAACTACTGAAAATGAATCTGCTAATGAGGGTTACAAATTTGGTC 5 0.125 No Hit GTACAAGCCTGTGGGGTCGCTTCTGCAACTGGATAACTAGCACTGAAAAT 5 0.125 No Hit GAAGCTTCTCTGATGGTATGCCTTTAGGAATATCTGGTACTTTCAACTTT 5 0.125 No Hit GGAAGCTGCATCCGTTGATGAATGGTTATACAATGGTGGTCCTTATGAGC 5 0.125 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 5 0.125 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0125 0.0 0.0 0.0 0.0 62-63 0.025 0.0 0.0 0.0 0.0 64-65 0.025 0.0 0.0 0.0 0.0 66-67 0.025 0.0 0.0 0.0 0.0 68-69 0.025 0.0 0.0 0.0 0.0 70-71 0.025 0.0 0.0 0.0 0.0 72-73 0.0625 0.0 0.0 0.0 0.0 74-75 0.125 0.0 0.0 0.0 0.0 76-77 0.1375 0.0 0.0 0.0 0.0 78-79 0.175 0.0 0.0 0.0 0.0 80-81 0.2625 0.0 0.0 0.0 0.0 82-83 0.35 0.0 0.0 0.0 0.0 84-85 0.425 0.0 0.0 0.0 0.0 86-87 0.5625 0.0 0.0 0.0 0.0 88-89 0.7250000000000001 0.0 0.0 0.0 0.0 90-91 0.875 0.0 0.0 0.0 0.0 92-93 0.9375 0.0 0.0 0.0 0.0 94-95 1.15 0.0 0.0 0.0 0.0 96-97 1.325 0.0 0.0 0.0 0.0 98-99 1.525 0.0 0.0 0.0 0.0 100-101 1.6375000000000002 0.0 0.0 0.0 0.0 102-103 1.8125 0.0 0.0 0.0 0.0 104-105 1.9500000000000002 0.0 0.0 0.0 0.0 106-107 2.275 0.0 0.0 0.0 0.0 108-109 2.65 0.0 0.0 0.0 0.0 110-111 2.9749999999999996 0.0 0.0 0.0 0.0 112-113 3.3875 0.0 0.0 0.0 0.0 114-115 3.75 0.0 0.0 0.0 0.0 116-117 4.175000000000001 0.0 0.0 0.0 0.0 118-119 4.5375 0.0 0.0 0.0 0.0 120-121 4.7875 0.0 0.0 0.0 0.0 122-123 5.0375 0.0 0.0 0.0 0.0 124-125 5.4625 0.0 0.0 0.0 0.0 126-127 5.875 0.0 0.0 0.0 0.0 128-129 6.175000000000001 0.0 0.0 0.0 0.0 130-131 6.625 0.0 0.0 0.0 0.0 132-133 7.05 0.0 0.0 0.0 0.0 134-135 7.5 0.0 0.0 0.0 0.0 136-137 7.9125 0.0 0.0 0.0 0.0 138-139 8.3 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TGTACGG 10 0.0065880334 146.71796 145 >>END_MODULE Read 1285303 spots for SRR6941577.sra Written 1285303 spots for SRR6941577.sra Read 1285303 spots for SRR6941577.sra Written 1285303 spots for SRR6941577.sra Read 1285303 spots for SRR6941577.sra Written 1285303 spots for SRR6941577.sra Read 1285303 spots for SRR6941577.sra Written 1285303 spots for SRR6941577.sra Read 1285303 spots for SRR6941577.sra Written 1285303 spots for SRR6941577.sra Read 1285308 spots for SRR6941577.sra Written 1285308 spots for SRR6941577.sra Read 1285303 spots for SRR6941577.sra Written 1285303 spots for SRR6941577.sra Read 1285303 spots for SRR6941577.sra Written 1285303 spots for SRR6941577.sra Read 1285303 spots for SRR6941577.sra Written 1285303 spots for SRR6941577.sra Read 1285303 spots for SRR6941577.sra Written 1285303 spots for SRR6941577.sra Read 1285303 spots for SRR6941577.sra Written 1285303 spots for SRR6941577.sra Read 1285303 spots for SRR6941577.sra Written 1285303 spots for SRR6941577.sra Read 1285303 spots for SRR6941577.sra Written 1285303 spots for SRR6941577.sra Read 1285303 spots for SRR6941577.sra Written 1285303 spots for SRR6941577.sra Read 1285303 spots for SRR6941577.sra Written 1285303 spots for SRR6941577.sra Read 1285303 spots for SRR6941577.sra Written 1285303 spots for SRR6941577.sra Read 1285303 spots for SRR6941577.sra Written 1285303 spots for SRR6941577.sra Read 1285303 spots for SRR6941577.sra Written 1285303 spots for SRR6941577.sra Read 1285303 spots for SRR6941577.sra Written 1285303 spots for SRR6941577.sra Read 1285303 spots for SRR6941577.sra Written 1285303 spots for SRR6941577.sra SRR ids: ['SRR6941577.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_4nllu0ew SRR6941577.sra spots: 25706065 blocks: [[1, 1285303], [1285304, 2570606], [2570607, 3855909], [3855910, 5141212], [5141213, 6426515], [6426516, 7711818], [7711819, 8997121], [8997122, 10282424], [10282425, 11567727], [11567728, 12853030], [12853031, 14138333], [14138334, 15423636], [15423637, 16708939], [16708940, 17994242], [17994243, 19279545], [19279546, 20564848], [20564849, 21850151], [21850152, 23135454], [23135455, 24420757], [24420758, 25706065]] SRR6941577 file size 8689241 SRR6941577 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR6941577 SRR6941577_1.fastq SRR6941577_2.fastq Input file: SRR6941577_1.fastq Paired file: SRR6941577_2.fastq trimmed: SRR6941577-trimmed-pair1.fastq, SRR6941577-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Fri Dec 6 11:55:58 2024 >> started Fri Dec 6 11:56:26 2024 >> done (28.430s) 25706065 read pairs processed; of these: 8854 ( 0.03%) short read pairs filtered out after trimming by size control 46575 ( 0.18%) empty read pairs filtered out after trimming by size control 25650636 (99.78%) read pairs available; of these: 4450625 (17.35%) trimmed read pairs available after processing 21200011 (82.65%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 4 0.00% 19 2 0.00% 20 2 0.00% 21 2 0.00% 22 3 0.00% 23 1 0.00% 24 4 0.00% 25 0 0.00% 26 3 0.00% 27 6 0.00% 28 5 0.00% 29 6 0.00% 30 10 0.00% 31 10 0.00% 32 17 0.00% 33 10 0.00% 34 21 0.00% 35 15 0.00% 36 33 0.00% 37 33 0.00% 38 44 0.00% 39 40 0.00% 40 69 0.00% 41 78 0.00% 42 78 0.00% 43 88 0.00% 44 80 0.00% 45 91 0.00% 46 112 0.00% 47 175 0.00% 48 190 0.00% 49 227 0.00% 50 252 0.00% 51 308 0.00% 52 329 0.00% 53 386 0.00% 54 374 0.00% 55 502 0.00% 56 524 0.00% 57 637 0.00% 58 715 0.00% 59 779 0.00% 60 891 0.00% 61 1159 0.00% 62 1395 0.01% 63 1497 0.01% 64 1697 0.01% 65 2012 0.01% 66 2052 0.01% 67 2276 0.01% 68 2641 0.01% 69 3009 0.01% 70 3505 0.01% 71 4324 0.02% 72 5043 0.02% 73 5407 0.02% 74 5450 0.02% 75 6878 0.03% 76 6573 0.03% 77 7386 0.03% 78 7599 0.03% 79 8069 0.03% 80 9201 0.04% 81 10140 0.04% 82 11611 0.05% 83 11782 0.05% 84 12769 0.05% 85 15502 0.06% 86 15886 0.06% 87 17094 0.07% 88 19117 0.07% 89 18692 0.07% 90 21222 0.08% 91 20771 0.08% 92 24781 0.10% 93 24879 0.10% 94 26564 0.10% 95 29065 0.11% 96 25989 0.10% 97 25881 0.10% 98 25481 0.10% 99 27148 0.11% 100 28203 0.11% 101 29758 0.12% 102 32659 0.13% 103 33072 0.13% 104 35528 0.14% 105 37645 0.15% 106 38941 0.15% 107 37649 0.15% 108 37806 0.15% 109 42665 0.17% 110 40632 0.16% 111 45778 0.18% 112 47561 0.19% 113 43480 0.17% 114 49246 0.19% 115 42490 0.17% 116 44848 0.17% 117 43038 0.17% 118 43067 0.17% 119 43903 0.17% 120 45436 0.18% 121 45702 0.18% 122 50386 0.20% 123 52075 0.20% 124 54743 0.21% 125 55124 0.21% 126 52756 0.21% 127 52844 0.21% 128 54875 0.21% 129 61714 0.24% 130 59576 0.23% 131 63847 0.25% 132 63311 0.25% 133 55530 0.22% 134 64195 0.25% 135 60915 0.24% 136 64306 0.25% 137 63344 0.25% 138 69024 0.27% 139 73780 0.29% 140 68704 0.27% 141 82796 0.32% 142 72960 0.28% 143 78879 0.31% 144 75019 0.29% 145 85294 0.33% 146 91686 0.36% 147 102167 0.40% 148 127417 0.50% 149 180212 0.70% 150 1045336 4.08% 151 21200011 82.65% 25650636 reads passed initial QC criterion=sequence-density sequence-density=0.35 sequence-density-rank=1 fanout-score=1.98 fanout-score-rank=31 prefix-density=0.35 prefix-fanout=2.0 sequence=TGCAGCGGCCCGTTATCCTTCCACCGTTGGAAGCGGGCAGTTGTCGCTGCTCTGTGAAGCCAGCCTCACGCTGTGCCTGCCAACATTATGGGCCGCGAAGCCTAGCTTTCGCTTAAGCTCCAACGGCCCACTACGCAACTTGGAACGGGCGGGCCATCAGTAGCACACCTAGACCAGGCCCGCAGCTCTACAGCAACGTCCACACCACCCTTAAAGCCCCCACTCGGGTTACAAGCTCTCGCTAC criterion=fanout-score sequence-density=0.01 sequence-density-rank=36 fanout-score=72.57 fanout-score-rank=1 prefix-density=0.09 prefix-fanout=5.5 sequence=AAAAAAAAAGGGGGGTAAGGACCCGCTAAGCTCCTACTTTTTCATGTTTCCAATCCGATCCCTCCGATTACTATAGAGATGAACCCAATCCAGAATATGAACCATAAAAGAAAACACCTACTAAACCAATCACAAGAATACCAGTTACCGTACCTATCAGCCAAAGAGGAATTCTTCCAGTAGTATCGGCCATTTCCCCTACTTTCCTCCACATTTTATCAAGTGGTCATGCTAGAGACAAAAACAGTCATGGATAGTTATGTTATAAGGATGGTATCCTTCCAAATGGGATAAGAGAGTTCTTACTACTCTCTTCTTTTCTCTCAATTAAAGAAGTAATTGGAAAACAAAACAGCAAGTACAAAAATGAGTAATAAACCCCAGTATAGACTGGTACGATTCAATTCAACATTTTGTTCATTCGGGTTTGATTGTGTCATAGTTCTATAGTTGGAATTTAGTTTATCGTTGGATGAACTGCATTGCTGATATTGATCCCAAGAAAAAAACA criterion=sequence-density sequence-density=0.30 sequence-density-rank=1 fanout-score=1.98 fanout-score-rank=29 prefix-density=0.30 prefix-fanout=2.0 sequence=GCGGCTCTCCTA criterion=fanout-score sequence-density=0.06 sequence-density-rank=24 fanout-score=20.38 fanout-score-rank=1 prefix-density=1.06 prefix-fanout=1.1 sequence=AAAAGATCGTGTATTTACAACTACAACGGAATAGTATACAAAGTCAACACCAACGATTAAATTGAATTTATGGCTACGCAAACCGTTGAAGATAGTTCTAAACCTAAACCAAGACGAACTGGTGCAGGTAGTTTATTGAAACCCTTGAATTCGGAATATGGGAAAGTAGCTCCAGGTTGGGGGACTACTCCTTTTATGGGGGTCGCAATGGCTTTATTCGCGATATTCCTATCTATCATTTTAGAAATTTATAATTCTTCTGTTTTATTGGACGGAATTTTAACCTATTAGGTTTCTACTAACTAAAAGTACGAAGTCGTAGTTTTTCCATCCAAAAAAAGCCTTTCTAGTTTAAGCTCTACATTTCTAGACATTCTGGTAGTTCGACCGCGGAATTTTTTTGTTTCGGTATCTCTGGAATATGAGTAAAGTATATGATTGGTTTGAGGAACGTCTTGAGATTCAGGCAATTGCAGATGATATAACTAGTAAATATGTTCCTCCTCATGTC SRR6941577 testing PE reads STAR mapping to Ensembl genome Started job on | Dec 06 11:57:01 Started mapping on | Dec 06 11:57:01 Finished on | Dec 06 11:58:55 Mapping speed, Million of reads per hour | 810.02 Number of input reads | 25650636 Average input read length | 294 UNIQUE READS: Uniquely mapped reads number | 15467016 Uniquely mapped reads % | 60.30% Average mapped length | 296.14 Number of splices: Total | 3102080 Number of splices: Annotated (sjdb) | 2788396 Number of splices: GT/AG | 2925231 Number of splices: GC/AG | 35695 Number of splices: AT/AC | 17026 Number of splices: Non-canonical | 124128 Mismatch rate per base, % | 0.25% Deletion rate per base | 0.02% Deletion average length | 1.93 Insertion rate per base | 0.02% Insertion average length | 2.66 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 9109400 % of reads mapped to multiple loci | 35.51% Number of reads mapped to too many loci | 22040 % of reads mapped to too many loci | 0.09% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 3.70% % of reads unmapped: other | 0.40% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 1079601 1079601 1079601 N_multimapping 9109400 9109400 9109400 N_noFeature 6669194 14847525 6935495 N_ambiguous 651161 10197 306808 UnstrandedReadsAssigned:8146661 PositiveStrandReadsAssigned:609294 NegativeStrandReadsAssigned:8224713 Dataset is classified negative stranded MeadianReadLen=151 20thPercentileLength=151 echo kmer=147 SRR6941577 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,972 [index] number of k-mers: 66,720,672 [index] number of equivalence classes: 111,837 [quant] running in paired-end mode [quant] will process pair 1: SRR6941577-trimmed-pair1.fastq SRR6941577-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 25,650,636 reads, 14,585,390 reads pseudoaligned [quant] estimated average fragment length: 237.972 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,089 rounds 52973 SRR6941577.ke.tsv 35125 SRR6941577.se.tsv 88098 total ==> SRR6941577.ke.tsv <== target_id length eff_length est_counts tpm PNS24245 936 699.521 0 0 PNS24247 1044 807.028 3.98048 0.330091 PNS24249 1928 1691.03 12.0803 0.478096 PNS24246 1044 807.028 3.98048 0.330091 PNS24248 1044 807.028 3.98048 0.330091 PNS24244 1471 1234.03 23.9783 1.30041 PNS24243 293 99.3895 0 0 KQK14069 1603 1366.03 351.453 17.2185 KQK14071 474 245.792 4.76599 1.29769 ==> SRR6941577.se.tsv <== BRADI_1g14170v3 526 BRADI_1g53295v3 33 BRADI_1g59795v3 11 BRADI_1g07683v3 0 BRADI_1g00485v3 1 BRADI_1g20270v3 94 BRADI_1g74790v3 17 BRADI_1g09890v3 0 BRADI_1g77505v3 37 BRADI_1g48960v3 0 SRR6941577 completed mapping pipeline successfully