Starting /dee2/code/volunteer_pipeline.sh SRR6941578 current disk space = 1551329189888 free memory = 1601457268 SRR6941578 SRAfilesize 813429a452f9a1419661f173915c7a89 SRR6941578.sra SRR6941578.sra file validated SRR6941578 is paired end SRR6941578 is conventional basespace SRR6941578 read1 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR6941578_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 34.6575 35.0 35.0 35.0 35.0 35.0 2 34.61125 35.0 35.0 35.0 35.0 35.0 3 34.6175 35.0 35.0 35.0 34.0 35.0 4 34.61475 35.0 35.0 35.0 35.0 35.0 5 34.58175 35.0 35.0 35.0 34.0 35.0 6 39.44975 40.0 40.0 40.0 39.0 40.0 7 39.4535 40.0 40.0 40.0 39.0 40.0 8 39.22475 40.0 40.0 40.0 39.0 40.0 9 39.442 40.0 40.0 40.0 39.0 40.0 10-14 39.44605 40.0 40.0 40.0 39.0 40.0 15-19 39.479699999999994 40.0 40.0 40.0 39.0 40.0 20-24 39.393299999999996 40.0 40.0 40.0 39.0 40.0 25-29 39.40175000000001 40.0 40.0 40.0 39.0 40.0 30-34 39.38875 40.0 40.0 40.0 39.0 40.0 35-39 39.36985 40.0 40.0 40.0 39.0 40.0 40-44 39.379099999999994 40.0 40.0 40.0 39.0 40.0 45-49 39.3321 40.0 40.0 40.0 39.0 40.0 50-54 39.3681 40.0 40.0 40.0 39.0 40.0 55-59 39.32705 40.0 40.0 40.0 39.0 40.0 60-64 39.3298 40.0 40.0 40.0 39.0 40.0 65-69 39.2983 40.0 40.0 40.0 39.0 40.0 70-74 39.314750000000004 40.0 40.0 40.0 39.0 40.0 75-79 39.22985 40.0 40.0 40.0 38.8 40.0 80-84 39.229200000000006 40.0 40.0 40.0 39.0 40.0 85-89 39.225550000000005 40.0 40.0 40.0 39.0 40.0 90-94 39.25915 40.0 40.0 40.0 39.0 40.0 95-99 39.11815 40.0 39.8 40.0 38.6 40.0 100-104 38.32595 39.4 38.6 39.6 37.0 39.8 105-109 39.07165 40.0 39.6 40.0 38.2 40.0 110-114 39.1938 40.0 40.0 40.0 39.0 40.0 115-119 39.1921 40.0 40.0 40.0 39.0 40.0 120-124 39.1472 40.0 40.0 40.0 39.0 40.0 125-129 39.022749999999995 40.0 40.0 40.0 38.4 40.0 130-134 39.0083 40.0 40.0 40.0 37.8 40.0 135-139 38.8548 40.0 39.2 40.0 37.4 40.0 140-144 38.83085 40.0 39.2 40.0 37.6 40.0 145-149 38.7188 40.0 39.2 40.0 37.2 40.0 150-151 37.298 39.5 37.5 40.0 34.0 40.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 14 1.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 2.0 21 0.0 22 2.0 23 0.0 24 1.0 25 5.0 26 5.0 27 3.0 28 4.0 29 15.0 30 18.0 31 32.0 32 22.0 33 36.0 34 49.0 35 71.0 36 83.0 37 142.0 38 290.0 39 3219.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 57.182251190774636 10.052644773126097 3.609927300075207 29.155176736024067 2 24.224999999999998 11.175 30.099999999999998 34.5 3 22.75 17.1 26.700000000000003 33.45 4 26.325 24.925 23.275000000000002 25.474999999999998 5 25.4 31.825 22.775000000000002 20.0 6 20.375 35.15 21.775 22.7 7 14.774999999999999 30.225 38.5 16.5 8 17.525 25.724999999999998 30.7 26.05 9 17.2 22.025 36.275 24.5 10-14 20.630000000000003 30.570000000000004 25.335 23.465 15-19 21.55 28.294999999999998 25.15 25.005 20-24 19.718943788757752 28.750750150030008 26.6003200640128 24.929985997199438 25-29 22.3 28.715000000000003 26.07 22.915 30-34 22.59 29.5 24.38 23.53 35-39 22.15 28.24 26.529999999999998 23.080000000000002 40-44 20.971291387416223 27.78333500050015 26.41292387716315 24.832449734920477 45-49 21.075 26.965 28.470000000000002 23.49 50-54 21.455 28.110000000000003 26.105 24.33 55-59 20.605 27.93 26.39 25.074999999999996 60-64 20.041002050102506 27.751387569378466 26.521326066303313 25.686284314215712 65-69 20.169999999999998 29.28 26.105 24.445 70-74 21.675 29.03 23.52 25.775 75-79 21.665 27.49 26.479999999999997 24.365000000000002 80-84 21.825 28.585 25.3 24.29 85-89 22.035 27.705000000000002 26.35 23.91 90-94 19.92099604980249 29.10145507275364 26.08130406520326 24.89624481224061 95-99 21.38 29.03 24.09 25.5 100-104 21.16 29.265 25.174999999999997 24.4 105-109 21.05 29.315 25.7 23.935000000000002 110-114 21.755 27.894999999999996 25.7 24.65 115-119 20.745 29.2 24.46 25.595000000000002 120-124 21.3 29.145 23.724999999999998 25.83 125-129 20.77 29.104999999999997 24.16 25.965 130-134 21.94 28.975 24.055 25.03 135-139 22.650000000000002 28.24 24.83 24.279999999999998 140-144 23.369999999999997 27.74 24.575 24.315 145-149 21.195 29.115000000000002 24.625 25.064999999999998 150-151 21.075 29.525000000000002 24.0 25.4 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.5 3 0.5 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.5 17 0.5 18 0.0 19 0.5 20 0.5 21 1.0 22 1.5 23 1.0 24 3.0 25 6.0 26 5.0 27 5.5 28 9.0 29 16.0 30 18.0 31 20.5 32 22.5 33 24.0 34 37.0 35 43.0 36 75.5 37 154.5 38 212.5 39 222.5 40 213.5 41 226.0 42 188.5 43 161.5 44 187.0 45 190.5 46 161.0 47 134.0 48 135.0 49 105.5 50 85.5 51 93.0 52 90.0 53 89.0 54 121.5 55 169.0 56 166.0 57 122.0 58 107.0 59 110.0 60 83.5 61 48.5 62 32.5 63 27.5 64 26.0 65 16.5 66 10.5 67 5.0 68 1.0 69 1.5 70 2.0 71 3.0 72 2.5 73 1.5 74 1.0 75 0.0 76 0.5 77 0.5 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.27499999999999997 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.02 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.03 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.005 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.005 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.7 #Duplication Level Percentage of deduplicated Percentage of total 1 82.21690590111643 51.55 2 8.572567783094097 10.75 3 3.4688995215311005 6.525 4 1.674641148325359 4.2 5 0.9968102073365231 3.125 6 0.7177033492822966 2.7 7 0.3987240829346092 1.7500000000000002 8 0.23923444976076555 1.2 9 0.27910685805422647 1.575 >10 1.3556618819776716 13.775 >50 0.07974481658692185 2.85 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GCGGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTACG 63 1.575 No Hit GGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTACGTT 51 1.275 No Hit GCTTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCA 41 1.0250000000000001 No Hit GGCCAAAATAACCATGAGCAGCCACAATATTATAAGTCTCTTCCTCTTGA 40 1.0 No Hit CTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCATTAAT 26 0.65 No Hit GCCAAAATAACCATGAGCAGCCACAATATTATAAGTCTCTTCCTCTTGAC 26 0.65 No Hit GTGCTCTGCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTCCTA 23 0.575 No Hit GTTGAAACTAGCATATTGGAAGATTAATCGGCCAAAATAACCATGAGCAG 21 0.525 No Hit GTGAGCATTACGTTCGTGCATTACTTCCATACCAAGATTAGCACGGTTGA 19 0.475 No Hit CTTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCAT 18 0.44999999999999996 No Hit GCTCTGCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTCCTAAA 18 0.44999999999999996 No Hit GCGACCTTGACTATCAACTACAGATTGGTTGAAATTGAATCCATTTAGGT 17 0.42500000000000004 No Hit GCATAAGGATGTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAAAGTA 17 0.42500000000000004 No Hit GCGGAAAAACCTTATCCATTAATAGCGGGAACTTCAAGAGCAGCTAGATC 17 0.42500000000000004 No Hit CCGGGAACGGATTCACCGCCGTATGGCTGACCGGCGATTACTAGCGATTC 16 0.4 No Hit GTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTC 15 0.375 No Hit GTTAGCGGAAAAACCTTATCCATTAATAGCGGGAACTTCAAGAGCAGCTA 15 0.375 No Hit GCGGTCAATAAGGTAGGGATCATCAAAACACCGAACCATCCGATGTAAAG 14 0.35000000000000003 No Hit GGATGCATAAGGATGTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAA 14 0.35000000000000003 No Hit CCCCACTGCTGCCTCCCGTAGGAGTCTGGGCCGTGTCTCAGTCCCAGTGT 14 0.35000000000000003 No Hit GTTGATGATATCAGCCCAAGTATTAATAACGCGACCTTGACTATCAACTA 13 0.325 No Hit GGAGGGGCTGCGATGAAGGCGATAATAAATACAGAAGTTGCGGTCAATAA 12 0.3 No Hit GCCGAAAACACCAGCTACACCTAACATGTGAAATGGATGCATAAGGATGT 12 0.3 No Hit GCTAGTTATCCAGTTGCAGAAGCGACCCCACAGGCTTGTACTTTCGCGTC 12 0.3 No Hit CTCTTGACCAAATTTGTAACCCTCATTAGCAGATTCATTTTCAGTAGTTT 12 0.3 No Hit GCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTCCTAAAGGCAT 12 0.3 No Hit GGCATGATGACTTGGCCTCATCCTCTCCTTCCTCCGGCTTAACACCGGCG 11 0.27499999999999997 No Hit GGGGCATGATGACTTGGCCTCATCCTCTCCTTCCTCCGGCTTAACACCGG 11 0.27499999999999997 No Hit GGCGATAATAAATACAGAAGTTGCGGTCAATAAGGTAGGGATCATCAAAA 11 0.27499999999999997 No Hit CCCAAGTATTAATAACGCGACCTTGACTATCAACTACAGATTGGTTGAAA 11 0.27499999999999997 No Hit CCCTCATTAGCAGATTCATTTTCAGTAGTTTCCCTGATCAAACTAGAGGT 11 0.27499999999999997 No Hit GTGTGTACAAGGCCCGGGAACGGATTCACCGCCGTATGGCTGACCGGCGA 11 0.27499999999999997 No Hit GCTGCGATGAAGGCGATAATAAATACAGAAGTTGCGGTCAATAAGGTAGG 11 0.27499999999999997 No Hit GCACGGTTGATGATATCAGCCCAAGTATTAATAACGCGACCTTGACTATC 10 0.25 No Hit ACATGCTCCACCGCTTGTGCGGGCCCCCGTCAATTCCTTTGAGTTTCATT 10 0.25 No Hit CTCTGCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTCCTAAAG 10 0.25 No Hit CGGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTACGT 9 0.22499999999999998 No Hit GGAAAAACCTTATCCATTAATAGCGGGAACTTCAAGAGCAGCTAGATCTA 9 0.22499999999999998 No Hit GCTGAATATGCAACAGCAATCCAAGGGCGCATACCCAAACGGAAACTAAG 9 0.22499999999999998 No Hit CCTCACGGTACTACTTCGCTATCGGTCACCCAGGAGTATTTAGCCTTGCA 9 0.22499999999999998 No Hit GTTCGCTGGAGTAGGCTGCGAGGCCCAAGCGGCCAAGGAGTGCAGCGGCC 9 0.22499999999999998 No Hit CTCATAAGGACCACCATTGTATAACCATTCATCAACGGATGCAGCTTCCC 9 0.22499999999999998 No Hit GTCCAGTCAACTGCTGCGCCTCAACGCATTTCGGGGAGAACCAGCTAGCT 9 0.22499999999999998 No Hit CAGAGAAGCTTCCTTGACCAATAGGGTAAATCAAGAAAACAGCAGTCGCA 8 0.2 No Hit GGTTGATGATATCAGCCCAAGTATTAATAACGCGACCTTGACTATCAACT 8 0.2 No Hit AGCGGAAAAACCTTATCCATTAATAGCGGGAACTTCAAGAGCAGCTAGAT 8 0.2 No Hit GCTTTCTTTTCCTCTGGCTACTAAGATGTTTCAGTTCGCCAGGTTGTCTC 8 0.2 No Hit GACCCATATAACAAGCTACACCAAGTAAGAAGTGTAGAACAATTAGCTCA 8 0.2 No Hit GCCAAGAAGAAGTGTAAAGAACGAGAGTTGTTGAAACTAGCATATTGGAA 8 0.2 No Hit CACCGCTTGTGCGGGCCCCCGTCAATTCCTTTGAGTTTCATTCTTGCGAA 7 0.17500000000000002 No Hit GTCTCTTCCTCTTGACCAAATTTGTAACCCTCATTAGCAGATTCATTTTC 7 0.17500000000000002 No Hit GCCACCTACAGACGCTTTACGCCCAATCATTCCGGATAACGCTTGCATCC 7 0.17500000000000002 No Hit CCCTACCGTACTCCAGCTTGGTAGTTTCCACCGCCTGTCCAGGGTTGAGC 7 0.17500000000000002 No Hit CCCACTGCTGCCTCCCGTAGGAGTCTGGGCCGTGTCTCAGTCCCAGTGTG 7 0.17500000000000002 No Hit CATAAGGATGTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAAAGTAC 7 0.17500000000000002 No Hit GTTGAAATTGAATCCATTTAGGTTGAACGCCATAGTACTAATACCTAAAG 7 0.17500000000000002 No Hit GCCCAAGTATTAATAACGCGACCTTGACTATCAACTACAGATTGGTTGAA 7 0.17500000000000002 No Hit CTCCACGCGGCATTGCTCCGTCAGGCTTTCGCCCATTGCGGAAAATTCCC 7 0.17500000000000002 No Hit ACCACATGCTCCACCGCTTGTGCGGGCCCCCGTCAATTCCTTTGAGTTTC 7 0.17500000000000002 No Hit GTGTGTACAAAGGGCAGGGACGTAGTCAACGCGAGCTGATGACTCGCGCT 6 0.15 No Hit ATTACGTTCGTGCATTACTTCCATACCAAGATTAGCACGGTTGATGATAT 6 0.15 No Hit CGAGAGTTGTTGAAACTAGCATATTGGAAGATTAATCGGCCAAAATAACC 6 0.15 No Hit GTGTACAAGGCCCGGGAACGGATTCACCGCCGTATGGCTGACCGGCGATT 6 0.15 No Hit GTCAATTCCTTTGAGTTTCATTCTTGCGAACGTACTCCCCAGGCGGGATA 6 0.15 No Hit GCATTACGTTCGTGCATTACTTCCATACCAAGATTAGCACGGTTGATGAT 6 0.15 No Hit GCTCCACCGCTTGTGCGGGCCCCCGTCAATTCCTTTGAGTTTCATTCTTG 6 0.15 No Hit GAGTTGTTGAAACTAGCATATTGGAAGATTAATCGGCCAAAATAACCATG 6 0.15 No Hit GTAGGGATCATCAAAACACCGAACCATCCGATGTAAAGACGATTTTCAGT 6 0.15 No Hit GTACAAGGCCCGGGAACGGATTCACCGCCGTATGGCTGACCGGCGATTAC 6 0.15 No Hit GATGCATAAGGATGTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAAA 6 0.15 No Hit GCTAGATCTAGAGGGAAGTTGTGAGCATTACGTTCGTGCATTACTTCCAT 6 0.15 No Hit CTCATTAGCAGATTCATTTTCAGTAGTTTCCCTGATCAAACTAGAGGTTA 6 0.15 No Hit GGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTACGTTC 6 0.15 No Hit GTGGGCATTACGTTCGTGCATTACTTCCATACCAAGATTAGCACGGTTGA 6 0.15 No Hit CCATGAGCAGCCACAATATTATAAGTCTCTTCCTCTTGACCAAATTTGTA 6 0.15 No Hit GGTAAATCAAGAAAACAGCAGTCGCAGCTGCAACAGGAGCTGAATATGCA 6 0.15 No Hit CTCGCGAATACCATCAATATCTACTGGAGGGGCTGCGATGAAGGCGATAA 6 0.15 No Hit CCCGAAGTTACGGGGCTATTTTGCCGAGTTCCTTAGAGAGAGTTGTCTCG 5 0.125 No Hit CCTAGATGTCCAGTCAACTGCTGCGCCTCAACGCATTTCGGGGAGAACCA 5 0.125 No Hit CCCATATAACAAGCTACACCAAGTAAGAAGTGTAGAACAATTAGCTCATA 5 0.125 No Hit GGAAGATTAATCGGCCAAAATAACCATGAGCAGCCACAATATTATAAGTC 5 0.125 No Hit CTTGACTATCAACTACAGATTGGTTGAAATTGAATCCATTTAGGTTGAAC 5 0.125 No Hit GACCACCATTGTATAACCATTCATCAACGGATGCAGCTTCCCAAATTGGG 5 0.125 No Hit GTTGCGGTCAATAAGGTAGGGATCATCAAAACACCGAACCATCCGATGTA 5 0.125 No Hit GGGAAGTTGTGAGCATTACGTTCGTGCATTACTTCCATACCAAGATTAGC 5 0.125 No Hit AACGAGAGTTGTTGAAACTAGCATATTGGAAGATTAATCGGCCAAAATAA 5 0.125 No Hit GCACTGAATAGGGAACCGCCGAAAACACCAGCTACACCTAACATGTGAAA 5 0.125 No Hit GGGCTGCGATGAAGGCGATAATAAATACAGAAGTTGCGGTCAATAAGGTA 5 0.125 No Hit GGTCAATAAGGTAGGGATCATCAAAACACCGAACCATCCGATGTAAAGAC 5 0.125 No Hit CCTTGACTATCAACTACAGATTGGTTGAAATTGAATCCATTTAGGTTGAA 5 0.125 No Hit CCTGCTTCATGCAGGCGAGTTGCAGCCTGCAATCCGAACTGAGGACGGGT 5 0.125 No Hit GCCACAATATTATAAGTCTCTTCCTCTTGACCAAATTTGTAACCCTCATT 5 0.125 No Hit CCCGTCAATTCCTTTGAGTTTCATTCTTGCGAACGTACTCCCCAGGCGGG 5 0.125 No Hit GCTCCCCTAGCTTTCGTCTCTCAGTGTCAGTGTCGGCCCAGCAGAGTGCT 5 0.125 No Hit CCACAATATTATAAGTCTCTTCCTCTTGACCAAATTTGTAACCCTCATTA 5 0.125 No Hit CCAAGTATTAATAACGCGACCTTGACTATCAACTACAGATTGGTTGAAAT 5 0.125 No Hit GTTTCCATAAAGTAAAGAACCAGAAACAGGCTCGCGAATACCATCAATAT 5 0.125 No Hit GTGACGGGCGGTGTGTACAAGGCCCGGGAACGGATTCACCGCCGTATGGC 5 0.125 No Hit GTCAATAAGGTAGGGATCATCAAAACACCGAACCATCCGATGTAAAGACG 5 0.125 No Hit GAGCATTACGTTCGTGCATTACTTCCATACCAAGATTAGCACGGTTGATG 5 0.125 No Hit ATAACAAGCTACACCAAGTAAGAAGTGTAGAACAATTAGCTCATAAGGAC 5 0.125 No Hit CCAGATATTCCTAAAGGCATACCATCAGAGAAGCTTCCTTGACCAATAGG 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0125 0.0 0.0 0.0 0.0 58-59 0.025 0.0 0.0 0.0 0.0 60-61 0.0875 0.0 0.0 0.0 0.0 62-63 0.1 0.0 0.0 0.0 0.0 64-65 0.1 0.0 0.0 0.0 0.0 66-67 0.1375 0.0 0.0 0.0 0.0 68-69 0.16249999999999998 0.0 0.0 0.0 0.0 70-71 0.175 0.0 0.0 0.0 0.0 72-73 0.2 0.0 0.0 0.0 0.0 74-75 0.3125 0.0 0.0 0.0 0.0 76-77 0.375 0.0 0.0 0.0 0.0 78-79 0.4 0.0 0.0 0.0 0.0 80-81 0.4625 0.0 0.0 0.0 0.0 82-83 0.6 0.0 0.0 0.0 0.0 84-85 0.7 0.0 0.0 0.0 0.0 86-87 0.8 0.0 0.0 0.0 0.0 88-89 0.9375 0.0 0.0 0.0 0.0 90-91 1.1375 0.0 0.0 0.0 0.0 92-93 1.2875 0.0 0.0 0.0 0.0 94-95 1.525 0.0 0.0 0.0 0.0 96-97 1.7125 0.0 0.0 0.0 0.0 98-99 1.9625 0.0 0.0 0.0 0.0 100-101 2.2625 0.0 0.0 0.0 0.0 102-103 2.5375 0.0 0.0 0.0 0.0 104-105 2.8 0.0 0.0 0.0 0.0 106-107 3.1875 0.0 0.0 0.0 0.0 108-109 3.55 0.0 0.0 0.0 0.0 110-111 3.9375 0.0 0.0 0.0 0.0 112-113 4.475 0.0 0.0 0.0 0.0 114-115 4.949999999999999 0.0 0.0 0.0 0.0 116-117 5.3625 0.0 0.0 0.0 0.0 118-119 5.5875 0.0 0.0 0.0 0.0 120-121 5.925000000000001 0.0 0.0 0.0 0.0 122-123 6.300000000000001 0.0 0.0 0.0 0.0 124-125 6.9375 0.0 0.0 0.0 0.0 126-127 7.4375 0.0 0.0 0.0 0.0 128-129 7.7375 0.0 0.0 0.0 0.0 130-131 8.3125 0.0 0.0 0.0 0.0 132-133 8.850000000000001 0.0 0.0 0.0 0.0 134-135 9.3375 0.0 0.0 0.0 0.0 136-137 9.9 0.0 0.0 0.0 0.0 138-139 10.2625 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCAAGGG 25 8.758201E-4 86.8875 145 GCGGGAA 35 0.003164506 62.8481 1 TGCTCTG 35 0.0033294512 62.0625 2 CGGGAAC 35 0.0033294512 62.0625 2 GCTCTGC 35 0.0033294512 62.0625 3 GTGCTCT 40 0.005370649 54.99209 1 CTCTGCC 40 0.005650197 54.304688 4 TGCCTGG 45 0.009003209 48.270832 7 TCTGCCT 45 0.009003209 48.270832 5 GGGAACT 45 0.009003209 48.270832 3 GCCTGGA 45 0.009003209 48.270832 8 CCTGGAA 45 0.009003209 48.270832 9 CGCAGCT 30 0.0014547207 24.135416 105-109 AATATGC 30 0.0014547207 24.135416 8 ACAGGAG 30 0.0014547207 24.135416 115-119 CTGCAAC 30 0.0014547207 24.135416 110-114 GCAGTCG 35 0.0035632392 20.6875 145 AGCTGAA 35 0.0035632392 20.6875 3 AGAGAAG 40 0.007169387 18.330696 60-64 CCATCAG 40 0.007491275 18.192526 55-59 >>END_MODULE SRR6941578 read2 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR6941578_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 34.1165 35.0 35.0 35.0 33.0 35.0 2 34.0355 35.0 35.0 35.0 33.0 35.0 3 33.88725 35.0 35.0 35.0 32.0 35.0 4 34.11525 35.0 35.0 35.0 33.0 35.0 5 34.23625 35.0 35.0 35.0 33.0 35.0 6 38.60825 40.0 39.0 40.0 37.0 40.0 7 38.9355 40.0 40.0 40.0 38.0 40.0 8 38.9895 40.0 40.0 40.0 38.0 40.0 9 39.02775 40.0 40.0 40.0 39.0 40.0 10-14 38.88420000000001 40.0 40.0 40.0 38.2 40.0 15-19 38.5974 40.0 39.8 40.0 36.8 40.0 20-24 38.94405 40.0 40.0 40.0 38.4 40.0 25-29 38.884299999999996 40.0 40.0 40.0 38.2 40.0 30-34 38.8549 40.0 40.0 40.0 37.8 40.0 35-39 38.8341 40.0 39.8 40.0 37.6 40.0 40-44 38.923199999999994 40.0 40.0 40.0 38.2 40.0 45-49 38.679199999999994 40.0 39.6 40.0 36.8 40.0 50-54 38.85355 40.0 40.0 40.0 37.8 40.0 55-59 38.80930000000001 40.0 39.6 40.0 37.4 40.0 60-64 38.852149999999995 40.0 40.0 40.0 38.0 40.0 65-69 38.7494 40.0 39.6 40.0 37.2 40.0 70-74 38.4833 40.0 39.0 40.0 36.2 40.0 75-79 38.71415 40.0 39.6 40.0 37.2 40.0 80-84 38.75285 40.0 39.4 40.0 37.4 40.0 85-89 38.38905 40.0 39.0 40.0 36.0 40.0 90-94 38.3959 40.0 39.0 40.0 36.2 40.0 95-99 38.28735 40.0 39.0 40.0 35.6 40.0 100-104 37.05775 38.6 37.8 39.4 33.4 39.4 105-109 37.967999999999996 40.0 39.0 40.0 35.2 40.0 110-114 37.8935 40.0 39.0 40.0 34.2 40.0 115-119 37.678599999999996 40.0 39.0 40.0 33.6 40.0 120-124 37.6708 40.0 39.0 40.0 33.8 40.0 125-129 37.306349999999995 40.0 38.8 40.0 33.2 40.0 130-134 37.66145 40.0 39.0 40.0 34.4 40.0 135-139 37.3582 40.0 39.0 40.0 33.8 40.0 140-144 37.02855 40.0 39.0 40.0 32.8 40.0 145-149 36.262249999999995 40.0 38.2 40.0 29.0 40.0 150-151 33.873375 38.5 34.0 39.5 16.0 40.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 12.0 3 0.0 4 1.0 5 0.0 6 1.0 7 0.0 8 0.0 9 1.0 10 1.0 11 1.0 12 0.0 13 0.0 14 1.0 15 5.0 16 1.0 17 2.0 18 3.0 19 1.0 20 7.0 21 4.0 22 18.0 23 9.0 24 11.0 25 8.0 26 23.0 27 27.0 28 18.0 29 29.0 30 34.0 31 42.0 32 41.0 33 48.0 34 80.0 35 109.0 36 121.0 37 196.0 38 378.0 39 2767.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 54.018644494834966 19.526329050138575 7.583774250440917 18.871252204585538 2 26.35542168674699 21.987951807228914 32.8062248995984 18.850401606425706 3 21.72172172172172 22.872872872872875 37.212212212212215 18.193193193193196 4 25.634581553154057 28.47449107816034 25.408394068861522 20.482533299824077 5 25.951903807615228 32.014028056112224 23.822645290581164 18.211422845691384 6 23.582538886101354 34.119417962870045 24.360260913196186 17.937782237832415 7 20.0 20.75187969924812 40.42606516290727 18.82205513784461 8 21.696696696696698 23.5985985985986 28.053053053053052 26.651651651651655 9 23.8988988988989 21.446446446446448 31.53153153153153 23.123123123123122 10-14 25.340681362725455 25.36072144288577 28.82264529058116 20.475951903807616 15-19 25.047638150636846 25.097783572359845 28.718283020760204 21.136295256243105 20-24 24.951169429558774 24.630640556918916 29.97946611909651 20.438723894425802 25-29 25.252929980967647 25.418210958629672 28.263047180206353 21.065811880196332 30-34 26.267580960008008 25.016267080434456 28.71014565293558 20.006006306621956 35-39 26.17688301282051 24.879807692307693 28.565705128205128 20.377604166666664 40-44 24.41907051282051 26.27704326923077 28.245192307692307 21.05869391025641 45-49 24.66453034247947 26.35189264970959 27.59363108351692 21.38994592429401 50-54 25.346822256723595 24.605599238743928 29.037912555716932 21.009665948815545 55-59 24.338942307692307 26.737780448717945 27.614182692307693 21.30909455128205 60-64 24.332849346617934 25.254093025584538 29.25950032543934 21.153557302358184 65-69 25.76235541535226 25.086375244103955 27.8754193580692 21.275849982474586 70-74 26.085432420251394 25.058841203865995 28.50418148129601 20.35154489458661 75-79 25.552271702649904 24.971196713920754 27.676200971797826 21.80033061163152 80-84 25.297887253429458 24.43176128967658 29.943927105236806 20.326424351657156 85-89 26.468085106382976 24.93617021276596 27.97496871088861 20.620775969962455 90-94 25.81226533166458 25.09136420525657 27.68961201501877 21.406758448060074 95-99 25.21147204564793 24.956204014214926 28.585014264978227 21.247309675158917 100-104 24.71538191484026 26.415567480816488 28.150860123376297 20.71819048096695 105-109 26.76282856712067 24.83288938030859 28.06955822485802 20.33472382771272 110-114 26.070782224009655 25.02010858636638 28.28272672431128 20.62638246531269 115-119 25.645145094889045 25.469424641028215 28.62737222612712 20.258058037955617 120-124 25.674315619967793 26.595209339774556 26.58011272141707 21.15036231884058 125-129 25.60076255455777 26.01715747754979 26.87503135503938 21.50704861285306 130-134 25.419555823535323 26.233544367400263 27.48467490704452 20.862224902019896 135-139 25.82658145035479 25.69573750691963 27.628201902269637 20.84947914045594 140-144 26.417850307242873 25.90409992948524 27.918807293240654 19.75924247003123 145-149 26.461863341545577 25.77806827894816 27.412137362361104 20.347931017145154 150-151 25.29212212589521 25.241864555848725 29.551451187335093 19.91456213092097 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 4.0 1 2.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.5 12 1.0 13 0.5 14 0.0 15 0.5 16 0.5 17 0.5 18 2.0 19 2.0 20 1.0 21 1.0 22 1.5 23 5.0 24 6.5 25 5.5 26 6.5 27 8.5 28 11.5 29 19.0 30 21.5 31 19.0 32 22.0 33 37.0 34 64.0 35 77.0 36 82.0 37 123.5 38 176.0 39 179.5 40 195.0 41 202.0 42 163.5 43 190.5 44 214.5 45 177.5 46 153.5 47 137.0 48 112.5 49 89.5 50 82.5 51 94.5 52 88.0 53 93.5 54 137.5 55 180.0 56 169.5 57 117.5 58 90.5 59 90.5 60 87.0 61 73.0 62 59.0 63 35.0 64 15.5 65 17.5 66 16.0 67 5.0 68 5.5 69 5.5 70 5.5 71 5.0 72 2.0 73 3.0 74 2.5 75 1.0 76 1.5 77 1.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.5 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.775 2 0.4 3 0.1 4 0.525 5 0.2 6 0.35000000000000003 7 0.25 8 0.1 9 0.1 10-14 0.2 15-19 0.29 20-24 0.165 25-29 0.16999999999999998 30-34 0.105 35-39 0.16 40-44 0.16 45-49 0.13999999999999999 50-54 0.165 55-59 0.16 60-64 0.135 65-69 0.145 70-74 0.155 75-79 0.185 80-84 0.13 85-89 0.125 90-94 0.125 95-99 0.105 100-104 0.305 105-109 0.515 110-114 0.54 115-119 0.41000000000000003 120-124 0.64 125-129 0.335 130-134 0.49 135-139 0.645 140-144 0.73 145-149 0.555 150-151 0.5125000000000001 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 65.14999999999999 #Duplication Level Percentage of deduplicated Percentage of total 1 81.35072908672295 53.0 2 8.710667689946277 11.35 3 4.182655410590944 8.175 4 1.8035303146584807 4.7 5 0.9976976208749041 3.25 6 0.6907137375287797 2.7 7 0.5755947812739831 2.625 8 0.38372985418265537 2.0 9 0.19186492709132769 1.125 >10 1.1128165771297005 11.075 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTTCGATCCTGGCTCAGGATGAACGCTGGCGGCATGCTTAACACATGCAA 30 0.75 No Hit GCTTGTTATATGGGTCGTGAGTGGGAACTTAGTTTCCGTTTGGGTATGCG 25 0.625 No Hit GGAAGCTTCTCTGATGGTATGCCTTTAGGAATATCTGGTACTTTCAACTT 23 0.575 No Hit ATTATCTATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAAT 23 0.575 No Hit GTGGGAACTTAGTTTCCGTTTGGGTATGCGCCCTTGGATTGCTGTTGCAT 21 0.525 No Hit GTTCAACCTAAATGGATTCAATTTCAACCAATCTGTAGTTGATAGTCAAG 20 0.5 No Hit GTTATTTTGGCCGATTAATCTTCCAATATGCTAGTTTCAACAACTCTCGT 18 0.44999999999999996 No Hit CTATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAA 17 0.42500000000000004 No Hit GGCTTGCGGTGGATACCTAGGCACCCAGAGACGAGGAAGGGCGTAGCAAG 17 0.42500000000000004 No Hit TATCTATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTT 17 0.42500000000000004 No Hit GCTAGTTTCAACAACTCTCGTTCTTTACACTTCTTCTTGGCTGCTTGGCC 16 0.4 No Hit GCTAATTGTTCTACACTTCTTACTTGGTGTAGCTTGTTATATGGGTCGTG 14 0.35000000000000003 No Hit GCACAACATCCTTATGCATCCATTTCACATGTTAGGTGTAGCTGGTGTTT 14 0.35000000000000003 No Hit GTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGATCTTACCATGAC 14 0.35000000000000003 No Hit AGAACCTTACCAGGGCTTGACATGCCGCGAATCCTCTTGAAAGAGAGGGG 14 0.35000000000000003 No Hit CCTTACCAGGGCTTGACATGCCGCGAATCCTCTTGAAAGAGAGGGGTGCC 13 0.325 No Hit ATCTATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTG 13 0.325 No Hit GGAAGAGACTTATAATATTGTGGCTGCTCATGGTTATTTTGGCCGATTAA 12 0.3 No Hit CCTGGCTCAGGATGAACGCTGGCGGCATGCTTAACACATGCAAGTCGAAC 12 0.3 No Hit GTTGCATATTCAGCTCCTGTTGCAGCTGCGACTGCTGTTTTCTTGATTTA 12 0.3 No Hit GTTTCAACAACTCTCGTTCTTTACACTTCTTCTTGGCTGCTTGGCCTGTA 12 0.3 No Hit GCTGCATCCGTTGATGAATGGTTATACAATGGTGGTCCTTATGAGCTAAT 12 0.3 No Hit GTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGATCTT 11 0.27499999999999997 No Hit GGGAAACTACTGAAAATGAATCTGCTAATGAGGGTTACAAATTTGGTCAA 11 0.27499999999999997 No Hit ATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAA 11 0.27499999999999997 No Hit AGGGAAACTACTGAAAATGAATCTGCTAATGAGGGTTACAAATTTGGTCA 11 0.27499999999999997 No Hit GTGGCTGCTCATGGTTATTTTGGCCGATTAATCTTCCAATATGCTAGTTT 10 0.25 No Hit CAGTGACCAGGCCCGGGCGACTGTTTACCAAAAACACAGGTCTCCGCAAA 10 0.25 No Hit GGTACTTTCAACTTTATGATTGTATTCCAGGCAGAGCACAACATCCTTAT 10 0.25 No Hit GTCGTGAGTGGGAACTTAGTTTCCGTTTGGGTATGCGCCCTTGGATTGCT 9 0.22499999999999998 No Hit GGAAACTACTGAAAATGAATCTGCTAATGAGGGTTACAAATTTGGTCAAG 9 0.22499999999999998 No Hit GTGCTATGCATGGTTCCTTGGTAACCTCTAGTTTGATCAGGGAAACTACT 9 0.22499999999999998 No Hit GTTGATAGTCAAGGTCGCGTTATTAATACTTGGGCTGATATCATCAACCG 9 0.22499999999999998 No Hit GTTTGATCAGGGAAACTACTGAAAATGAATCTGCTAATGAGGGTTACAAA 9 0.22499999999999998 No Hit AGCACAACATCCTTATGCATCCATTTCACATGTTAGGTGTAGCTGGTGTT 8 0.2 No Hit GAACCTTACCAGGGCTTGACATGCCGCGAATCCTCTTGAAAGAGAGGGGT 8 0.2 No Hit GTACTATGGCGTTCAACCTAAATGGATTCAATTTCAACCAATCTGTAGTT 8 0.2 No Hit ATTTGGTCAAGAGGAAGAGACTTATAATATTGTGGCTGCTCATGGTTATT 8 0.2 No Hit CTTTAGGAATATCTGGTACTTTCAACTTTATGATTGTATTCCAGGCAGAG 8 0.2 No Hit AAACAATATTATCTCTGGTGCTATTATTCCTACTTCTGCGGCAATCGGAT 8 0.2 No Hit GTAGCTTGTTATATGGGTCGTGAGTGGGAACTTAGTTTCCGTTTGGGTAT 8 0.2 No Hit GTAGGAATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCT 8 0.2 No Hit GAGGAAGAGACTTATAATATTGTGGCTGCTCATGGTTATTTTGGCCGATT 8 0.2 No Hit GTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGATCTTACCATGACTG 8 0.2 No Hit GTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCTAAATGGATTCA 7 0.17500000000000002 No Hit TATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAA 7 0.17500000000000002 No Hit CCAAGATCTTACCATGACTGCAATTTTAGAGAGACGCGAAAGTACAAGCC 7 0.17500000000000002 No Hit AAACTACTGAAAATGAATCTGCTAATGAGGGTTACAAATTTGGTCAAGAG 7 0.17500000000000002 No Hit GGCCTGTAGTAGGAATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCG 7 0.17500000000000002 No Hit GCATGGTTCCTTGGTAACCTCTAGTTTGATCAGGGAAACTACTGAAAATG 7 0.17500000000000002 No Hit CTTACCAGGGCTTGACATGCCGCGAATCCTCTTGAAAGAGAGGGGTGCCC 7 0.17500000000000002 No Hit GTTATACAATGGTGGTCCTTATGAGCTAATTGTTCTACACTTCTTACTTG 7 0.17500000000000002 No Hit GGAATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCTAAA 7 0.17500000000000002 No Hit ATTCAGTGCTATGCATGGTTCCTTGGTAACCTCTAGTTTGATCAGGGAAA 7 0.17500000000000002 No Hit GTTGGGTTAAGTCTCGCAACGAGCGCAACCCTCGTGTTTAGTTGCCACTA 7 0.17500000000000002 No Hit GCTGCGACTGCTGTTTTCTTGATTTACCCTATTGGTCAAGGAAGCTTCTC 7 0.17500000000000002 No Hit ATTGTATTCCAGGCAGAGCACAACATCCTTATGCATCCATTTCACATGTT 7 0.17500000000000002 No Hit GAAGAGACTTATAATATTGTGGCTGCTCATGGTTATTTTGGCCGATTAAT 7 0.17500000000000002 No Hit GTACAAGCCTGTGGGGTCGCTTCTGCAACTGGATAACTAGCACTGAAAAT 7 0.17500000000000002 No Hit GGTCGCTTCTGCAACTGGATAACTAGCACTGAAAATCGTCTTTACATCGG 6 0.15 No Hit GAGCTAATTGTTCTACACTTCTTACTTGGTGTAGCTTGTTATATGGGTCG 6 0.15 No Hit CTGCATCCGTTGATGAATGGTTATACAATGGTGGTCCTTATGAGCTAATT 6 0.15 No Hit CTGGTACTTTCAACTTTATGATTGTATTCCAGGCAGAGCACAACATCCTT 6 0.15 No Hit GTTAAGTCTCGCAACGAGCGCAACCCTCGTGTTTAGTTGCCACTATGAGT 6 0.15 No Hit ATCGCCTTCATCGCAGCCCCTCCAGTAGATATTGATGGTATTCGCGAGCC 6 0.15 No Hit GACTTATAATATTGTGGCTGCTCATGGTTATTTTGGCCGATTAATCTTCC 6 0.15 No Hit GTACGGTAGGGGCAGAGGGAATTTCCGGTGGAGCGGTGAAATGCATTGAG 6 0.15 No Hit GTCGAACGGGAAGTGGTGTTTCCAGTGGCGAACGGGTGAGTAACGCGTAA 6 0.15 No Hit GCTGTTTTCTTGATTTACCCTATTGGTCAAGGAAGCTTCTCTGATGGTAT 6 0.15 No Hit GTTTTGATGATCCCTACCTTATTGACCGCAACTTCTGTATTTATTATCGC 6 0.15 No Hit GCCTGTAGTAGGAATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGT 6 0.15 No Hit GGTTCCTTGGTAACCTCTAGTTTGATCAGGGAAACTACTGAAAATGAATC 6 0.15 No Hit GTTAGGTGTAGCTGGTGTTTTCGGCGGTTCCCTATTCAGTGCTATGCATG 6 0.15 No Hit GTGTTTTCGGCGGTTCCCTATTCAGTGCTATGCATGGTTCCTTGGTAACC 6 0.15 No Hit TCTATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGA 6 0.15 No Hit GCTTTAGGTATTAGTACTATGGCGTTCAACCTAAATGGATTCAATTTCAA 6 0.15 No Hit GAATGGTTATACAATGGTGGTCCTTATGAGCTAATTGTTCTACACTTCTT 6 0.15 No Hit GTTTGGGTATGCGCCCTTGGATTGCTGTTGCATATTCAGCTCCTGTTGCA 5 0.125 No Hit CATGGTTATTTTGGCCGATTAATCTTCCAATATGCTAGTTTCAACAACTC 5 0.125 No Hit GTCCTTATGAGCTAATTGTTCTACACTTCTTACTTGGTGTAGCTTGTTAT 5 0.125 No Hit GTCTTTACATCGGATGGTTCGGTGTTTTGATGATCCCTACCTTATTGACC 5 0.125 No Hit CAACTCTCGTTCTTTACACTTCTTCTTGGCTGCTTGGCCTGTAGTAGGAA 5 0.125 No Hit GGTGTTTTCGGCGGTTCCCTATTCAGTGCTATGCATGGTTCCTTGGTAAC 5 0.125 No Hit GCCTGACGGAGCAATGCCGCGTGGAGGTGGAAGGCCTACGGGTCGTCAAC 5 0.125 No Hit GTTCGGTGTTTTGATGATCCCTACCTTATTGACCGCAACTTCTGTATTTA 5 0.125 No Hit TAGGAATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCTA 5 0.125 No Hit GATTAATCTTCCAATATGCTAGTTTCAACAACTCTCGTTCTTTACACTTC 5 0.125 No Hit GTACTTTCAACTTTATGATTGTATTCCAGGCAGAGCACAACATCCTTATG 5 0.125 No Hit CTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCTAAATGGAT 5 0.125 No Hit GGTTATTTTGGCCGATTAATCTTCCAATATGCTAGTTTCAACAACTCTCG 5 0.125 No Hit GCTTGCGGTGGATACCTAGGCACCCAGAGACGAGGAAGGGCGTAGCAAGC 5 0.125 No Hit GTGACCAGGCCCGGGCGACTGTTTACCAAAAACACAGGTCTCCGCAAAGT 5 0.125 No Hit GTTACAAATTTGGTCAAGAGGAAGAGACTTATAATATTGTGGCTGCTCAT 5 0.125 No Hit GTTATATGGGTCGTGAGTGGGAACTTAGTTTCCGTTTGGGTATGCGCCCT 5 0.125 No Hit GGAAGGCCTACGGGTCGTCAACTTCTTTTCTCGGAGAAGAAACAATGACG 5 0.125 No Hit AGCAAAAGGAGAAATCCGCCCAAGGAGGGGCTCGCGTCTGATTAGCTAGT 5 0.125 No Hit GCTTGACATGCCGCGAATCCTCTTGAAAGAGAGGGGTGCCCTCGGGAACG 5 0.125 No Hit GTTGATGAATGGTTATACAATGGTGGTCCTTATGAGCTAATTGTTCTACA 5 0.125 No Hit CAGGGAAACTACTGAAAATGAATCTGCTAATGAGGGTTACAAATTTGGTC 5 0.125 No Hit AAGGCTTGCGGTGGATACCTAGGCACCCAGAGACGAGGAAGGGCGTAGCA 5 0.125 No Hit GAGCACAACATCCTTATGCATCCATTTCACATGTTAGGTGTAGCTGGTGT 5 0.125 No Hit GTTCTTTACTTTATGGAAACAATATTATCTCTGGTGCTATTATTCCTACT 5 0.125 No Hit GCTAACTCCAAAAACCCGTCCTCAGTTCGGATTGCAGGCTGCAACTCGCC 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0125 0.0 0.0 0.0 0.0 58-59 0.025 0.0 0.0 0.0 0.0 60-61 0.0875 0.0 0.0 0.0 0.0 62-63 0.1 0.0 0.0 0.0 0.0 64-65 0.1 0.0 0.0 0.0 0.0 66-67 0.1375 0.0 0.0 0.0 0.0 68-69 0.16249999999999998 0.0 0.0 0.0 0.0 70-71 0.175 0.0 0.0 0.0 0.0 72-73 0.2 0.0 0.0 0.0 0.0 74-75 0.3125 0.0 0.0 0.0 0.0 76-77 0.375 0.0 0.0 0.0 0.0 78-79 0.4 0.0 0.0 0.0 0.0 80-81 0.4625 0.0 0.0 0.0 0.0 82-83 0.6 0.0 0.0 0.0 0.0 84-85 0.7 0.0 0.0 0.0 0.0 86-87 0.825 0.0 0.0 0.0 0.0 88-89 0.9624999999999999 0.0 0.0 0.0 0.0 90-91 1.1625 0.0 0.0 0.0 0.0 92-93 1.3125 0.0 0.0 0.0 0.0 94-95 1.5499999999999998 0.0 0.0 0.0 0.0 96-97 1.7125 0.0 0.0 0.0 0.0 98-99 1.9625 0.0 0.0 0.0 0.0 100-101 2.2375 0.0 0.0 0.0 0.0 102-103 2.4749999999999996 0.0 0.0 0.0 0.0 104-105 2.725 0.0 0.0 0.0 0.0 106-107 3.0875 0.0 0.0 0.0 0.0 108-109 3.4000000000000004 0.0 0.0 0.0 0.0 110-111 3.7874999999999996 0.0 0.0 0.0 0.0 112-113 4.3 0.0 0.0 0.0 0.0 114-115 4.775 0.0 0.0 0.0 0.0 116-117 5.175000000000001 0.0 0.0 0.0 0.0 118-119 5.387499999999999 0.0 0.0 0.0 0.0 120-121 5.725 0.0 0.0 0.0 0.0 122-123 6.1 0.0 0.0 0.0 0.0 124-125 6.7375 0.0 0.0 0.0 0.0 126-127 7.2375 0.0 0.0 0.0 0.0 128-129 7.55 0.0 0.0 0.0 0.0 130-131 8.1125 0.0 0.0 0.0 0.0 132-133 8.6625 0.0 0.0 0.0 0.0 134-135 9.1625 0.0 0.0 0.0 0.0 136-137 9.7375 0.0 0.0 0.0 0.0 138-139 10.1125 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content pass >>END_MODULE Read 1236879 spots for SRR6941578.sra Written 1236879 spots for SRR6941578.sra Read 1236879 spots for SRR6941578.sra Written 1236879 spots for SRR6941578.sra Read 1236879 spots for SRR6941578.sra Written 1236879 spots for SRR6941578.sra Read 1236879 spots for SRR6941578.sra Written 1236879 spots for SRR6941578.sra Read 1236879 spots for SRR6941578.sra Written 1236879 spots for SRR6941578.sra Read 1236879 spots for SRR6941578.sra Written 1236879 spots for SRR6941578.sra Read 1236879 spots for SRR6941578.sra Written 1236879 spots for SRR6941578.sra Read 1236879 spots for SRR6941578.sra Written 1236879 spots for SRR6941578.sra Read 1236879 spots for SRR6941578.sra Written 1236879 spots for SRR6941578.sra Read 1236879 spots for SRR6941578.sra Written 1236879 spots for SRR6941578.sra Read 1236879 spots for SRR6941578.sra Written 1236879 spots for SRR6941578.sra Read 1236879 spots for SRR6941578.sra Written 1236879 spots for SRR6941578.sra Read 1236879 spots for SRR6941578.sra Written 1236879 spots for SRR6941578.sra Read 1236879 spots for SRR6941578.sra Written 1236879 spots for SRR6941578.sra Read 1236879 spots for SRR6941578.sra Written 1236879 spots for SRR6941578.sra Read 1236879 spots for SRR6941578.sra Written 1236879 spots for SRR6941578.sra Read 1236879 spots for SRR6941578.sra Written 1236879 spots for SRR6941578.sra Read 1236879 spots for SRR6941578.sra Written 1236879 spots for SRR6941578.sra Read 1236879 spots for SRR6941578.sra Written 1236879 spots for SRR6941578.sra Read 1236898 spots for SRR6941578.sra Written 1236898 spots for SRR6941578.sra SRR ids: ['SRR6941578.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_23fdywgo SRR6941578.sra spots: 24737599 blocks: [[1, 1236879], [1236880, 2473758], [2473759, 3710637], [3710638, 4947516], [4947517, 6184395], [6184396, 7421274], [7421275, 8658153], [8658154, 9895032], [9895033, 11131911], [11131912, 12368790], [12368791, 13605669], [13605670, 14842548], [14842549, 16079427], [16079428, 17316306], [17316307, 18553185], [18553186, 19790064], [19790065, 21026943], [21026944, 22263822], [22263823, 23500701], [23500702, 24737599]] SRR6941578 file size 8361060 SRR6941578 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR6941578 SRR6941578_1.fastq SRR6941578_2.fastq Input file: SRR6941578_1.fastq Paired file: SRR6941578_2.fastq trimmed: SRR6941578-trimmed-pair1.fastq, SRR6941578-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Fri Dec 6 11:59:13 2024 >> started Fri Dec 6 11:59:38 2024 >> done (25.054s) 24737599 read pairs processed; of these: 13112 ( 0.05%) short read pairs filtered out after trimming by size control 28522 ( 0.12%) empty read pairs filtered out after trimming by size control 24695965 (99.83%) read pairs available; of these: 5613430 (22.73%) trimmed read pairs available after processing 19082535 (77.27%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 3 0.00% 19 1 0.00% 20 2 0.00% 21 9 0.00% 22 6 0.00% 23 3 0.00% 24 12 0.00% 25 7 0.00% 26 19 0.00% 27 20 0.00% 28 15 0.00% 29 23 0.00% 30 31 0.00% 31 34 0.00% 32 41 0.00% 33 32 0.00% 34 41 0.00% 35 49 0.00% 36 47 0.00% 37 62 0.00% 38 81 0.00% 39 86 0.00% 40 105 0.00% 41 120 0.00% 42 138 0.00% 43 149 0.00% 44 158 0.00% 45 165 0.00% 46 219 0.00% 47 278 0.00% 48 245 0.00% 49 327 0.00% 50 379 0.00% 51 432 0.00% 52 505 0.00% 53 524 0.00% 54 598 0.00% 55 681 0.00% 56 746 0.00% 57 877 0.00% 58 987 0.00% 59 1130 0.00% 60 1206 0.00% 61 1560 0.01% 62 1893 0.01% 63 2060 0.01% 64 2388 0.01% 65 2586 0.01% 66 2720 0.01% 67 2938 0.01% 68 3687 0.01% 69 4230 0.02% 70 4506 0.02% 71 5110 0.02% 72 6238 0.03% 73 6626 0.03% 74 6469 0.03% 75 7401 0.03% 76 7664 0.03% 77 8893 0.04% 78 8518 0.03% 79 9687 0.04% 80 10924 0.04% 81 12097 0.05% 82 13800 0.06% 83 14412 0.06% 84 15252 0.06% 85 18267 0.07% 86 18327 0.07% 87 19700 0.08% 88 22577 0.09% 89 22030 0.09% 90 24758 0.10% 91 25588 0.10% 92 29685 0.12% 93 30261 0.12% 94 31818 0.13% 95 33697 0.14% 96 32578 0.13% 97 33229 0.13% 98 32905 0.13% 99 34809 0.14% 100 35873 0.15% 101 39097 0.16% 102 42570 0.17% 103 41996 0.17% 104 44511 0.18% 105 45084 0.18% 106 44927 0.18% 107 45585 0.18% 108 47639 0.19% 109 52546 0.21% 110 50690 0.21% 111 56181 0.23% 112 57565 0.23% 113 53838 0.22% 114 56909 0.23% 115 54423 0.22% 116 55482 0.22% 117 53150 0.22% 118 53828 0.22% 119 52066 0.21% 120 54551 0.22% 121 55670 0.23% 122 63847 0.26% 123 67546 0.27% 124 67394 0.27% 125 70359 0.28% 126 64998 0.26% 127 63386 0.26% 128 63212 0.26% 129 69311 0.28% 130 67746 0.27% 131 72849 0.29% 132 71216 0.29% 133 65660 0.27% 134 73288 0.30% 135 72275 0.29% 136 76072 0.31% 137 74655 0.30% 138 81289 0.33% 139 81247 0.33% 140 76813 0.31% 141 91911 0.37% 142 83836 0.34% 143 90027 0.36% 144 89689 0.36% 145 104550 0.42% 146 112846 0.46% 147 121302 0.49% 148 152010 0.62% 149 223654 0.91% 150 1523780 6.17% 151 19082535 77.27% 24695965 reads passed initial QC criterion=sequence-density sequence-density=0.38 sequence-density-rank=1 fanout-score=2.40 fanout-score-rank=25 prefix-density=0.39 prefix-fanout=2.3 sequence=GTTACGGCCGCCGTTCACCGGGGCTTCGGTCGCCGGCTTCCCTGTCATCAGTTCACCAACTTCCTTGACCTTCCGGCACTGGGCAGGCGTCAGCCCCCATACATGGTCTTACGACTTTGCGGAGACCTGTGTTTTTGGTAAACAGTCGCCCGGGCCTGGTCACTGC criterion=fanout-score sequence-density=0.01 sequence-density-rank=43 fanout-score=46.17 fanout-score-rank=1 prefix-density=0.11 prefix-fanout=3.8 sequence=ATAAAAAAAAGGGGGGGTAAGGACCCGCTAAGCTCCTACTTTTTCATGTTTCCAATCCGATCCCTCCGATTACTATAGAGATGAACCCAATCCAGAATATGAACCATAAAAGAAAACACCTACTAAACCAATCACAAGAATACCAGTTACCGTACCTATCAGCCAAAGAGGAATTCTTCCAGTAGTATCGGCCATTTCCCCTACTTTCCTCCACATTTTATCAAGTGGTCATGCTAGAGACAAAAACAGTCATGGATAGTTATGTTATAAGGATGGTATCCTTCCAAATGGGATAAGAGAGTTCTTACTACTCTCTTCTTTTCTCTCAATTAAAGAAGTAATTGGAAAACAAAACAGCAAGTACAAAAATGAGTAATAAACCCCAGTATAGACTGGTACGATTCAATTCAACATTTTGTTCATTCGGGTTTGATTGTGTCATAGTTCTATAGTTGGAATTTAGTTTATCGTTGGATGAACTGCATTGCTGATATTGATCCCAAGAAAAAAA criterion=sequence-density sequence-density=0.28 sequence-density-rank=1 fanout-score=2.31 fanout-score-rank=26 prefix-density=0.28 prefix-fanout=2.3 sequence=ATAACGGTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGACCCGCACGAAAGGCGTAACGATCTGGGCACTGTCTCGGAGAGAGGCTCGGTGAAATAGACATGTCTGTGAAGATGCGGACTACCTGCACCTGGACAGAAAGACCCTATGAAGCTTTACTGTTCCCTGGGATTGGCTTTGGGCCTTTCCTGCGCAGCTTAGGTGGAAGGCGAAGAAGGCCCCCTTCCGGGGGGGCCCGAGCCATCAGTGAGATACCACTCTGGAAGAGCTCGGATTCTAACCTTGTGTCAGACCCGCGGGCCAAGGGACAGTCTCAGGTAGACAGTTTCTATGGGGCGTAGGCCTCCCAAAAGGTAACGGAGGCGTGCAAAGGTTTCCTCGGGCCAGACGGACATTGGTCCTCGAGTGCAAAGGCAGAAGGGAGCTTGACTGCAAGACTCACCCGTCGAGCAGAGACGAAAGTCGGCCTTAGTGATCCGACGGTGCCGAGTGGAAGGGCCGTCGCTC criterion=fanout-score sequence-density=0.08 sequence-density-rank=24 fanout-score=37.62 fanout-score-rank=1 prefix-density=2.90 prefix-fanout=1.0 sequence=GCGAACGGGTGCGTAACGCGTGGGAATCTGCCGAACAGTTCGGGCCAAATCCTGAAGAAAGCTCAAAAGCGCTGTTTGATGAGCCTGCGTAGTATTAGGTAGTTGGTCAGGTAAAGGCTGACCAAGCCAATGATGCTTAGCTGGTCTTTTCGGATGATCAGC SRR6941578 testing PE reads STAR mapping to Ensembl genome Started job on | Dec 06 12:00:30 Started mapping on | Dec 06 12:00:31 Finished on | Dec 06 12:02:43 Mapping speed, Million of reads per hour | 673.53 Number of input reads | 24695965 Average input read length | 292 UNIQUE READS: Uniquely mapped reads number | 13591628 Uniquely mapped reads % | 55.04% Average mapped length | 294.36 Number of splices: Total | 2545111 Number of splices: Annotated (sjdb) | 2222632 Number of splices: GT/AG | 2342525 Number of splices: GC/AG | 30863 Number of splices: AT/AC | 10771 Number of splices: Non-canonical | 160952 Mismatch rate per base, % | 0.15% Deletion rate per base | 0.01% Deletion average length | 1.52 Insertion rate per base | 0.01% Insertion average length | 1.98 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 8379186 % of reads mapped to multiple loci | 33.93% Number of reads mapped to too many loci | 176761 % of reads mapped to too many loci | 0.72% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 7.18% % of reads unmapped: other | 3.13% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 2734730 2734730 2734730 N_multimapping 8379186 8379186 8379186 N_noFeature 6868340 13177432 7062024 N_ambiguous 457741 4370 243666 UnstrandedReadsAssigned:6265547 PositiveStrandReadsAssigned:409826 NegativeStrandReadsAssigned:6285938 Dataset is classified negative stranded MeadianReadLen=151 20thPercentileLength=151 echo kmer=147 SRR6941578 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,972 [index] number of k-mers: 66,720,672 [index] number of equivalence classes: 111,837 [quant] running in paired-end mode [quant] will process pair 1: SRR6941578-trimmed-pair1.fastq SRR6941578-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 24,695,965 reads, 11,334,102 reads pseudoaligned [quant] estimated average fragment length: 223.6 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,026 rounds 52973 SRR6941578.ke.tsv 35125 SRR6941578.se.tsv 88098 total ==> SRR6941578.ke.tsv <== target_id length eff_length est_counts tpm PNS24245 936 713.768 0 0 PNS24247 1044 821.4 10.4988 0.797478 PNS24249 1928 1705.4 13.6365 0.498896 PNS24246 1044 821.4 10.4988 0.797478 PNS24248 1044 821.4 10.4988 0.797478 PNS24244 1471 1248.4 28.8671 1.44273 PNS24243 293 107.827 0 0 KQK14069 1603 1380.4 2337.57 105.656 KQK14071 474 260.374 53.2137 12.7515 ==> SRR6941578.se.tsv <== BRADI_1g14170v3 3174 BRADI_1g53295v3 10 BRADI_1g59795v3 89 BRADI_1g07683v3 0 BRADI_1g00485v3 0 BRADI_1g20270v3 44 BRADI_1g74790v3 8 BRADI_1g09890v3 0 BRADI_1g77505v3 55 BRADI_1g48960v3 0 SRR6941578 completed mapping pipeline successfully