Starting /dee2/code/volunteer_pipeline.sh SRR6941579 current disk space = 1551312797696 free memory = 1337458036 SRR6941579 SRAfilesize 540d70db9ba36755635dfb0f69990d7b SRR6941579.sra SRR6941579.sra file validated SRR6941579 is paired end SRR6941579 is conventional basespace SRR6941579 read1 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR6941579_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 34.49925 35.0 35.0 35.0 35.0 35.0 2 34.48725 35.0 35.0 35.0 34.0 35.0 3 34.6035 35.0 35.0 35.0 34.0 35.0 4 34.61 35.0 35.0 35.0 35.0 35.0 5 34.55625 35.0 35.0 35.0 34.0 35.0 6 39.35875 40.0 40.0 40.0 39.0 40.0 7 39.3905 40.0 40.0 40.0 39.0 40.0 8 39.19125 40.0 40.0 40.0 39.0 40.0 9 39.3985 40.0 40.0 40.0 39.0 40.0 10-14 39.37845 40.0 40.0 40.0 39.0 40.0 15-19 39.17315 40.0 40.0 40.0 38.6 40.0 20-24 39.3369 40.0 40.0 40.0 39.0 40.0 25-29 39.3384 40.0 40.0 40.0 39.0 40.0 30-34 39.248749999999994 40.0 40.0 40.0 39.0 40.0 35-39 39.2379 40.0 40.0 40.0 39.0 40.0 40-44 39.306 40.0 40.0 40.0 39.0 40.0 45-49 39.24745 40.0 40.0 40.0 38.8 40.0 50-54 39.1799 40.0 40.0 40.0 38.8 40.0 55-59 39.1835 40.0 40.0 40.0 38.6 40.0 60-64 39.14455 40.0 40.0 40.0 38.2 40.0 65-69 39.1871 40.0 40.0 40.0 38.6 40.0 70-74 39.10785 40.0 40.0 40.0 38.2 40.0 75-79 39.04045 40.0 39.8 40.0 37.8 40.0 80-84 39.0394 40.0 40.0 40.0 38.0 40.0 85-89 38.877700000000004 40.0 39.2 40.0 37.0 40.0 90-94 39.0059 40.0 39.6 40.0 38.0 40.0 95-99 38.810249999999996 40.0 39.0 40.0 36.8 40.0 100-104 38.2028 39.4 38.4 39.8 35.8 39.8 105-109 38.83855 40.0 39.0 40.0 36.8 40.0 110-114 38.849900000000005 40.0 39.0 40.0 36.8 40.0 115-119 38.8505 40.0 39.0 40.0 36.8 40.0 120-124 38.69075 40.0 39.0 40.0 36.6 40.0 125-129 38.56345 40.0 39.0 40.0 36.0 40.0 130-134 38.472500000000004 40.0 39.0 40.0 36.0 40.0 135-139 38.4236 40.0 39.0 40.0 36.0 40.0 140-144 38.242 40.0 39.0 40.0 35.4 40.0 145-149 37.863 40.0 39.0 40.0 34.6 40.0 150-151 35.497375 38.5 35.0 39.5 25.0 40.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 21 1.0 22 0.0 23 0.0 24 2.0 25 2.0 26 4.0 27 7.0 28 24.0 29 30.0 30 27.0 31 35.0 32 34.0 33 49.0 34 69.0 35 94.0 36 108.0 37 163.0 38 320.0 39 3031.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 43.07576517812343 11.51530356246864 6.82388359257401 38.585047666833916 2 23.446893787575153 13.802605210420843 32.43987975951904 30.31062124248497 3 22.400000000000002 17.224999999999998 25.275 35.099999999999994 4 26.650000000000002 26.05 21.275 26.025 5 23.779724655819777 34.1927409261577 21.8523153942428 20.175219023779725 6 20.275000000000002 37.425000000000004 23.0 19.3 7 13.475000000000001 32.2 39.475 14.85 8 18.0 27.700000000000003 31.4 22.900000000000002 9 16.35 25.15 36.3 22.2 10-14 18.765 34.39 25.369999999999997 21.475 15-19 19.85 31.69 25.240000000000002 23.22 20-24 17.88 31.605 27.685 22.830000000000002 25-29 21.975 31.55 26.295 20.18 30-34 21.959999999999997 32.455 24.474999999999998 21.11 35-39 20.705000000000002 31.965 26.205000000000002 21.125 40-44 19.185 31.695 26.650000000000002 22.470000000000002 45-49 19.650000000000002 30.5 27.91 21.94 50-54 20.925 31.785000000000004 26.305 20.985 55-59 21.05 30.099999999999998 25.035 23.815 60-64 18.634999999999998 31.290000000000003 27.284999999999997 22.79 65-69 20.27 31.740000000000002 25.919999999999998 22.07 70-74 19.814999999999998 31.635 24.245 24.305 75-79 20.495 30.595 27.025 21.884999999999998 80-84 21.64 30.91 25.11 22.34 85-89 21.315 30.28 26.33 22.075 90-94 19.115 30.880000000000003 26.740000000000002 23.265 95-99 19.86 32.065 24.4 23.674999999999997 100-104 20.005 32.300000000000004 25.4 22.295 105-109 19.64 31.474999999999998 26.465 22.42 110-114 20.794999999999998 30.135 25.94 23.13 115-119 19.75 32.225 24.82 23.205000000000002 120-124 19.74 31.89 23.72 24.65 125-129 19.78 32.105 23.915 24.2 130-134 21.88 31.985000000000003 23.799999999999997 22.335 135-139 22.040000000000003 30.975 24.44 22.545 140-144 22.64 30.964999999999996 25.264999999999997 21.13 145-149 20.11 31.95 24.349999999999998 23.59 150-151 20.74787393696848 32.54127063531766 23.19909954977489 23.51175587793897 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.5 4 0.5 5 0.0 6 0.5 7 0.5 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.5 20 1.5 21 2.0 22 3.5 23 4.0 24 4.5 25 5.5 26 5.5 27 8.5 28 13.0 29 20.5 30 23.5 31 22.5 32 26.5 33 27.5 34 36.5 35 46.5 36 94.0 37 239.0 38 302.0 39 250.5 40 267.0 41 308.5 42 270.0 43 279.5 44 273.0 45 230.0 46 199.0 47 161.5 48 144.0 49 88.5 50 64.0 51 60.5 52 50.0 53 49.0 54 47.5 55 56.5 56 63.5 57 47.0 58 40.5 59 39.5 60 30.0 61 25.5 62 18.0 63 11.5 64 13.5 65 8.0 66 2.5 67 1.0 68 2.5 69 3.0 70 1.5 71 1.0 72 1.0 73 0.5 74 0.5 75 0.5 76 0.0 77 0.0 78 0.5 79 0.5 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.35000000000000003 2 0.2 3 0.0 4 0.0 5 0.125 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.05 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.85 #Duplication Level Percentage of deduplicated Percentage of total 1 83.64831552999178 50.9 2 7.559572719802794 9.2 3 2.4650780608052587 4.5 4 1.561216105176664 3.8 5 1.0271158586688578 3.125 6 0.903861955628595 3.3000000000000003 7 0.4519309778142975 1.925 8 0.3697617091207888 1.7999999999999998 9 0.3697617091207888 2.025 >10 1.561216105176664 16.475 >50 0.08216926869350863 2.9499999999999997 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GCGGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTACG 59 1.4749999999999999 No Hit CTTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCAT 59 1.4749999999999999 No Hit GGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTACGTT 46 1.15 No Hit GCTTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCA 38 0.95 No Hit CCCAAGTATTAATAACGCGACCTTGACTATCAACTACAGATTGGTTGAAA 34 0.8500000000000001 No Hit CTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCATTAAT 33 0.8250000000000001 No Hit GGCCAAAATAACCATGAGCAGCCACAATATTATAAGTCTCTTCCTCTTGA 33 0.8250000000000001 No Hit GTTAGCGGAAAAACCTTATCCATTAATAGCGGGAACTTCAAGAGCAGCTA 30 0.75 No Hit CTCATAAGGACCACCATTGTATAACCATTCATCAACGGATGCAGCTTCCC 26 0.65 No Hit GTGCTCTGCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTCCTA 25 0.625 No Hit CGGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTACGT 22 0.5499999999999999 No Hit GCGGAAAAACCTTATCCATTAATAGCGGGAACTTCAAGAGCAGCTAGATC 22 0.5499999999999999 No Hit GCCAAAATAACCATGAGCAGCCACAATATTATAAGTCTCTTCCTCTTGAC 18 0.44999999999999996 No Hit GACCCATATAACAAGCTACACCAAGTAAGAAGTGTAGAACAATTAGCTCA 18 0.44999999999999996 No Hit GGAGGGGCTGCGATGAAGGCGATAATAAATACAGAAGTTGCGGTCAATAA 16 0.4 No Hit CCAGATTCCTACTACAGGCCAAGCAGCCAAGAAGAAGTGTAAAGAACGAG 16 0.4 No Hit AGCGGAAAAACCTTATCCATTAATAGCGGGAACTTCAAGAGCAGCTAGAT 16 0.4 No Hit CGCGACCTTGACTATCAACTACAGATTGGTTGAAATTGAATCCATTTAGG 15 0.375 No Hit GCTCTGCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTCCTAAA 15 0.375 No Hit CCACAATATTATAAGTCTCTTCCTCTTGACCAAATTTGTAACCCTCATTA 13 0.325 No Hit GCATAAGGATGTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAAAGTA 13 0.325 No Hit GTTGAAATTGAATCCATTTAGGTTGAACGCCATAGTACTAATACCTAAAG 13 0.325 No Hit GTTGAAACTAGCATATTGGAAGATTAATCGGCCAAAATAACCATGAGCAG 13 0.325 No Hit GTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTC 12 0.3 No Hit GCTGCGATGAAGGCGATAATAAATACAGAAGTTGCGGTCAATAAGGTAGG 12 0.3 No Hit CTCTTGACCAAATTTGTAACCCTCATTAGCAGATTCATTTTCAGTAGTTT 12 0.3 No Hit CTCTGCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTCCTAAAG 12 0.3 No Hit TTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCATT 11 0.27499999999999997 No Hit TTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCATTA 11 0.27499999999999997 No Hit GGATGCATAAGGATGTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAA 11 0.27499999999999997 No Hit GGTCAATAAGGTAGGGATCATCAAAACACCGAACCATCCGATGTAAAGAC 11 0.27499999999999997 No Hit CCAAGTATTAATAACGCGACCTTGACTATCAACTACAGATTGGTTGAAAT 11 0.27499999999999997 No Hit GCCAAGAAGAAGTGTAAAGAACGAGAGTTGTTGAAACTAGCATATTGGAA 11 0.27499999999999997 No Hit GGCGATAATAAATACAGAAGTTGCGGTCAATAAGGTAGGGATCATCAAAA 10 0.25 No Hit GAGGGAAGTTGTGAGCATTACGTTCGTGCATTACTTCCATACCAAGATTA 10 0.25 No Hit GTGAGCATTACGTTCGTGCATTACTTCCATACCAAGATTAGCACGGTTGA 10 0.25 No Hit CATATAACAAGCTACACCAAGTAAGAAGTGTAGAACAATTAGCTCATAAG 10 0.25 No Hit CATCAGAGAAGCTTCCTTGACCAATAGGGTAAATCAAGAAAACAGCAGTC 10 0.25 No Hit ATCAATATCTACTGGAGGGGCTGCGATGAAGGCGATAATAAATACAGAAG 10 0.25 No Hit GGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTACGTTC 10 0.25 No Hit CGCAGAAGTAGGAATAATAGCACCAGAGATAATATTGTTTCCATAAAGTA 9 0.22499999999999998 No Hit CTTGACTATCAACTACAGATTGGTTGAAATTGAATCCATTTAGGTTGAAC 9 0.22499999999999998 No Hit CCACCATTGTATAACCATTCATCAACGGATGCAGCTTCCCAAATTGGGTA 9 0.22499999999999998 No Hit GTTGATGATATCAGCCCAAGTATTAATAACGCGACCTTGACTATCAACTA 9 0.22499999999999998 No Hit GATGCATAAGGATGTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAAA 9 0.22499999999999998 No Hit CTCATTAGCAGATTCATTTTCAGTAGTTTCCCTGATCAAACTAGAGGTTA 9 0.22499999999999998 No Hit CACCATTGTATAACCATTCATCAACGGATGCAGCTTCCCAAATTGGGTAA 9 0.22499999999999998 No Hit CCCCACTGCTGCCTCCCGTAGGAGTCTGGGCCGTGTCTCAGTCCCAGTGT 9 0.22499999999999998 No Hit GGGGCTGCGATGAAGGCGATAATAAATACAGAAGTTGCGGTCAATAAGGT 9 0.22499999999999998 No Hit GCCGAAAACACCAGCTACACCTAACATGTGAAATGGATGCATAAGGATGT 8 0.2 No Hit GGAAAAACCTTATCCATTAATAGCGGGAACTTCAAGAGCAGCTAGATCTA 8 0.2 No Hit CCCTCATTAGCAGATTCATTTTCAGTAGTTTCCCTGATCAAACTAGAGGT 8 0.2 No Hit GTAAAGAACGAGAGTTGTTGAAACTAGCATATTGGAAGATTAATCGGCCA 8 0.2 No Hit CATCAATATCTACTGGAGGGGCTGCGATGAAGGCGATAATAAATACAGAA 8 0.2 No Hit GCCCAAGTATTAATAACGCGACCTTGACTATCAACTACAGATTGGTTGAA 8 0.2 No Hit CCATGAGCAGCCACAATATTATAAGTCTCTTCCTCTTGACCAAATTTGTA 8 0.2 No Hit CTTATCCATTAATAGCGGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAG 8 0.2 No Hit CTCGCGAATACCATCAATATCTACTGGAGGGGCTGCGATGAAGGCGATAA 8 0.2 No Hit CCCATATAACAAGCTACACCAAGTAAGAAGTGTAGAACAATTAGCTCATA 7 0.17500000000000002 No Hit ACCAGATATTCCTAAAGGCATACCATCAGAGAAGCTTCCTTGACCAATAG 7 0.17500000000000002 No Hit GGGTAATGTTGCTCCAATACCTAACCAAAGAGCTACTGCAGTACCGATTA 7 0.17500000000000002 No Hit GGGAAGTTGTGAGCATTACGTTCGTGCATTACTTCCATACCAAGATTAGC 7 0.17500000000000002 No Hit GCGACCTTGACTATCAACTACAGATTGGTTGAAATTGAATCCATTTAGGT 7 0.17500000000000002 No Hit CTTATATGTTAGCGGAAAAACCTTATCCATTAATAGCGGGAACTTCAAGA 7 0.17500000000000002 No Hit GGCCAAGCAGCCAAGAAGAAGTGTAAAGAACGAGAGTTGTTGAAACTAGC 7 0.17500000000000002 No Hit CACGGTTGATGATATCAGCCCAAGTATTAATAACGCGACCTTGACTATCA 7 0.17500000000000002 No Hit GCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTCCTAAAGGCAT 7 0.17500000000000002 No Hit GGAATAATAGCACCAGAGATAATATTGTTTCCATAAAGTAAAGAACCAGA 7 0.17500000000000002 No Hit GAGCATTACGTTCGTGCATTACTTCCATACCAAGATTAGCACGGTTGATG 7 0.17500000000000002 No Hit GCGGTCAATAAGGTAGGGATCATCAAAACACCGAACCATCCGATGTAAAG 6 0.15 No Hit CGCCATAGTACTAATACCTAAAGCAGTGAACCAGATTCCTACTACAGGCC 6 0.15 No Hit GTGTAAAGAACGAGAGTTGTTGAAACTAGCATATTGGAAGATTAATCGGC 6 0.15 No Hit CTGCGATGAAGGCGATAATAAATACAGAAGTTGCGGTCAATAAGGTAGGG 6 0.15 No Hit CTTCCTCTTGACCAAATTTGTAACCCTCATTAGCAGATTCATTTTCAGTA 6 0.15 No Hit GGTTGATGATATCAGCCCAAGTATTAATAACGCGACCTTGACTATCAACT 6 0.15 No Hit ACGAGAGTTGTTGAAACTAGCATATTGGAAGATTAATCGGCCAAAATAAC 6 0.15 No Hit TGATGATATCAGCCCAAGTATTAATAACGCGACCTTGACTATCAACTACA 6 0.15 No Hit CCATATAACAAGCTACACCAAGTAAGAAGTGTAGAACAATTAGCTCATAA 6 0.15 No Hit CCTACTACAGGCCAAGCAGCCAAGAAGAAGTGTAAAGAACGAGAGTTGTT 6 0.15 No Hit CCAAGAAGAAGTGTAAAGAACGAGAGTTGTTGAAACTAGCATATTGGAAG 6 0.15 No Hit ATCGGCCAAAATAACCATGAGCAGCCACAATATTATAAGTCTCTTCCTCT 6 0.15 No Hit GCTAGTTATCCAGTTGCAGAAGCGACCCCACAGGCTTGTACTTTCGCGTC 6 0.15 No Hit TTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCATTAATA 6 0.15 No Hit GTCCATTAATAGCGGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTG 6 0.15 No Hit CTAGCATATTGGAAGATTAATCGGCCAAAATAACCATGAGCAGCCACAAT 6 0.15 No Hit CATAAGGATGTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAAAGTAC 6 0.15 No Hit CTAGAGGGAAGTTGTGAGCATTACGTTCGTGCATTACTTCCATACCAAGA 6 0.15 No Hit CGGTCAATAAGGTAGGGATCATCAAAACACCGAACCATCCGATGTAAAGA 6 0.15 No Hit AGTTGTTGAAACTAGCATATTGGAAGATTAATCGGCCAAAATAACCATGA 6 0.15 No Hit CATGAGCAGCCACAATATTATAAGTCTCTTCCTCTTGACCAAATTTGTAA 6 0.15 No Hit CCTCATTAGCAGATTCATTTTCAGTAGTTTCCCTGATCAAACTAGAGGTT 6 0.15 No Hit GCACGGTTGATGATATCAGCCCAAGTATTAATAACGCGACCTTGACTATC 5 0.125 No Hit CCGCAGAAGTAGGAATAATAGCACCAGAGATAATATTGTTTCCATAAAGT 5 0.125 No Hit CAGCCAAGAAGAAGTGTAAAGAACGAGAGTTGTTGAAACTAGCATATTGG 5 0.125 No Hit GGCGCATACCCAAACGGAAACTAAGTTCCCACTCACGACCCATATAACAA 5 0.125 No Hit CGAGAGTTGTTGAAACTAGCATATTGGAAGATTAATCGGCCAAAATAACC 5 0.125 No Hit TCTGCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTCCTAAAGG 5 0.125 No Hit TGGAGGGGCTGCGATGAAGGCGATAATAAATACAGAAGTTGCGGTCAATA 5 0.125 No Hit GTTGCGATGAAGGCGATAATAAATACAGAAGTTGCGGTCAATAAGGTAGG 5 0.125 No Hit GCATATTGGAAGATTAATCGGCCAAAATAACCATGAGCAGCCACAATATT 5 0.125 No Hit AGGAATAATAGCACCAGAGATAATATTGTTTCCATAAAGTAAAGAACCAG 5 0.125 No Hit GGAAGTTGTGAGCATTACGTTCGTGCATTACTTCCATACCAAGATTAGCA 5 0.125 No Hit CCTTGACTATCAACTACAGATTGGTTGAAATTGAATCCATTTAGGTTGAA 5 0.125 No Hit CGCATACCCAAACGGAAACTAAGTTCCCACTCACGACCCATATAACAAGC 5 0.125 No Hit GTGCTAGTTATCCAGTTGCAGAAGCGACCCCACAGGCTTGTACTTTCGCG 5 0.125 No Hit GACCTTGACTATCAACTACAGATTGGTTGAAATTGAATCCATTTAGGTTG 5 0.125 No Hit CCAGAGATAATATTGTTTCCATAAAGTAAAGAACCAGAAACAGGCTCGCG 5 0.125 No Hit ATTAATAGCGGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAG 5 0.125 No Hit GTGAAATGGATGCATAAGGATGTTGTGCTCTGCCTGGAATACAATCATAA 5 0.125 No Hit GTAGGAATAATAGCACCAGAGATAATATTGTTTCCATAAAGTAAAGAACC 5 0.125 No Hit GTGGGCATTACGTTCGTGCATTACTTCCATACCAAGATTAGCACGGTTGA 5 0.125 No Hit GGTAAATCAAGAAAACAGCAGTCGCAGCTGCAACAGGAGCTGAATATGCA 5 0.125 No Hit CCACAGGCTTGTACTTTCGCGTCTCTCTAAAATTGCAGTCATGGTAAGAT 5 0.125 No Hit CGGCCAAAATAACCATGAGCAGCCACAATATTATAAGTCTCTTCCTCTTG 5 0.125 No Hit GGACCACCATTGTATAACCATTCATCAACGGATGCAGCTTCCCAAATTGG 5 0.125 No Hit CTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCATTAATAGCGG 5 0.125 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.037500000000000006 0.0 0.0 0.0 0.0 66-67 0.05 0.0 0.0 0.0 0.0 68-69 0.075 0.0 0.0 0.0 0.0 70-71 0.075 0.0 0.0 0.0 0.0 72-73 0.0875 0.0 0.0 0.0 0.0 74-75 0.1125 0.0 0.0 0.0 0.0 76-77 0.21250000000000002 0.0 0.0 0.0 0.0 78-79 0.275 0.0 0.0 0.0 0.0 80-81 0.3375 0.0 0.0 0.0 0.0 82-83 0.4125 0.0 0.0 0.0 0.0 84-85 0.4875 0.0 0.0 0.0 0.0 86-87 0.5625 0.0 0.0 0.0 0.0 88-89 0.625 0.0 0.0 0.0 0.0 90-91 0.7 0.0 0.0 0.0 0.0 92-93 0.825 0.0 0.0 0.0 0.0 94-95 1.025 0.0 0.0 0.0 0.0 96-97 1.225 0.0 0.0 0.0 0.0 98-99 1.35 0.0 0.0 0.0 0.0 100-101 1.5 0.0 0.0 0.0 0.0 102-103 1.675 0.0 0.0 0.0 0.0 104-105 1.9625 0.0 0.0 0.0 0.0 106-107 2.3 0.0 0.0 0.0 0.0 108-109 2.5875 0.0 0.0 0.0 0.0 110-111 2.8375 0.0 0.0 0.0 0.0 112-113 3.2125 0.0 0.0 0.0 0.0 114-115 3.4749999999999996 0.0 0.0 0.0 0.0 116-117 3.675 0.0 0.0 0.0 0.0 118-119 4.0 0.0 0.0 0.0 0.0 120-121 4.35 0.0 0.0 0.0 0.0 122-123 4.7625 0.0 0.0 0.0 0.0 124-125 5.15 0.0 0.0 0.0 0.0 126-127 5.6375 0.0 0.0 0.0 0.0 128-129 6.125 0.0 0.0 0.0 0.0 130-131 6.4625 0.0 0.0 0.0 0.0 132-133 6.8375 0.0 0.0 0.0 0.0 134-135 7.35 0.0 0.0 0.0 0.0 136-137 7.9 0.0 0.0 0.0 0.0 138-139 8.3625 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTTTTCT 20 1.8793453E-6 145.0 5 TTTCTTT 20 1.8793453E-6 145.0 2 TTTTCTT 20 1.8793453E-6 145.0 6 TTCTTTT 20 1.8793453E-6 145.0 3 TCTTTTC 20 1.8793453E-6 145.0 4 CTTTCTT 20 1.8793453E-6 145.0 1 TTTCTTC 25 5.7044963E-6 116.0 7 TTCTTCA 25 5.7044963E-6 116.0 8 TCTTCAA 25 5.7044963E-6 116.0 9 GTGCTCT 40 0.005621335 54.375 1 GATATCA 70 9.353284E-4 41.428574 145 TAGCGGA 20 0.00593511 29.0 30-34 CTTCAAA 20 0.00593511 29.0 10-14 TATATGT 20 0.00593511 29.0 20-24 TTCAAAA 25 4.977651E-4 29.0 10-14 TATGTTA 20 0.00593511 29.0 25-29 TGTTAGC 20 0.00593511 29.0 25-29 AGCGGAA 20 0.00593511 29.0 30-34 TTCTTAT 20 0.00593511 29.0 15-19 CTTATAT 20 0.00593511 29.0 20-24 >>END_MODULE SRR6941579 read2 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR6941579_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 33.95225 35.0 35.0 35.0 32.0 35.0 2 34.165 35.0 35.0 35.0 33.0 35.0 3 34.17 35.0 35.0 35.0 33.0 35.0 4 34.048 35.0 35.0 35.0 32.0 35.0 5 34.15925 35.0 35.0 35.0 33.0 35.0 6 38.87875 40.0 40.0 40.0 37.0 40.0 7 38.858 40.0 40.0 40.0 37.0 40.0 8 38.87175 40.0 40.0 40.0 38.0 40.0 9 38.8875 40.0 40.0 40.0 37.0 40.0 10-14 38.929500000000004 40.0 40.0 40.0 37.8 40.0 15-19 38.94015 40.0 40.0 40.0 37.8 40.0 20-24 38.92545 40.0 40.0 40.0 37.8 40.0 25-29 38.88405 40.0 40.0 40.0 37.8 40.0 30-34 38.82135 40.0 39.8 40.0 37.4 40.0 35-39 38.870999999999995 40.0 40.0 40.0 37.8 40.0 40-44 38.714999999999996 40.0 39.4 40.0 36.8 40.0 45-49 38.52695 40.0 39.0 40.0 36.2 40.0 50-54 38.58365 40.0 39.0 40.0 36.2 40.0 55-59 38.5963 40.0 39.0 40.0 36.2 40.0 60-64 38.5181 40.0 39.0 40.0 36.2 40.0 65-69 38.43525 40.0 39.0 40.0 36.0 40.0 70-74 38.4191 40.0 39.0 40.0 36.0 40.0 75-79 38.2468 40.0 39.0 40.0 35.4 40.0 80-84 38.307900000000004 40.0 39.0 40.0 35.8 40.0 85-89 38.23055 40.0 39.0 40.0 35.6 40.0 90-94 38.13265 40.0 39.0 40.0 35.2 40.0 95-99 38.0698 40.0 39.0 40.0 34.8 40.0 100-104 37.1738 39.0 38.0 39.6 32.8 39.8 105-109 37.58985 40.0 39.0 40.0 34.0 40.0 110-114 34.3345 36.4 34.2 38.4 26.8 38.6 115-119 18.504749999999998 16.6 15.6 23.4 12.8 30.0 120-124 2.0 2.0 2.0 2.0 2.0 2.0 125-129 2.0 2.0 2.0 2.0 2.0 2.0 130-134 2.0 2.0 2.0 2.0 2.0 2.0 135-139 2.0 2.0 2.0 2.0 2.0 2.0 140-144 2.0 2.0 2.0 2.0 2.0 2.0 145-149 2.0 2.0 2.0 2.0 2.0 2.0 150-151 2.0 2.0 2.0 2.0 2.0 2.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 0.0 4 1.0 5 0.0 6 1.0 7 1.0 8 1.0 9 4.0 10 0.0 11 0.0 12 2.0 13 2.0 14 4.0 15 7.0 16 6.0 17 12.0 18 15.0 19 23.0 20 18.0 21 23.0 22 32.0 23 54.0 24 53.0 25 62.0 26 77.0 27 94.0 28 176.0 29 271.0 30 2000.0 31 1060.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 38.7606623181134 19.568489713998996 14.024084295032612 27.64676367285499 2 24.34194033592379 22.63725244422161 37.6034093757834 15.417397844071196 3 18.07470543995989 24.667836550513915 40.08523439458511 17.17222361494109 4 23.289044873401853 30.960140386061667 25.770869892203557 19.979944848332913 5 24.843319127600903 30.960140386061667 28.052143394334422 16.144397092003008 6 20.549999999999997 34.175 27.775 17.5 7 16.950000000000003 21.85 45.125 16.075 8 21.099999999999998 25.7 31.2 22.0 9 22.650000000000002 20.65 34.949999999999996 21.75 10-14 22.13 26.729999999999997 32.214999999999996 18.925 15-19 23.625 24.81 32.195 19.37 20-24 22.695 25.83 32.16 19.314999999999998 25-29 22.855 24.735 32.515 19.895 30-34 24.135 24.315 32.23 19.32 35-39 24.15224567370211 24.492347704311292 32.06962088626588 19.28578573572072 40-44 22.703622173303984 25.48028817290374 31.72903742245347 20.087052231338802 45-49 22.752118750313425 26.42294769570232 31.217090416729356 19.607843137254903 50-54 23.25266796933714 25.948193797284436 31.068690816173156 19.730447417205273 55-59 22.367563774870945 26.742845687365307 30.21600761790207 20.673582919861673 60-64 22.182710608033656 25.292998096764503 31.86917760192327 20.655113693278572 65-69 23.072301221710394 25.52072902062888 30.67794912878029 20.72902062888043 70-74 23.173601722497622 25.296680186270095 31.886235040809176 19.643483050423114 75-79 23.079615923184637 24.70494098819764 31.321264252850572 20.894178835767153 80-84 23.293293293293292 23.613613613613612 33.84384384384384 19.24924924924925 85-89 24.482344703411023 26.177853356006803 30.03401020306092 19.305791737521254 90-94 23.400000000000002 25.069999999999997 30.48 21.05 95-99 22.634999999999998 25.715 31.275 20.375 100-104 23.77618880944047 25.636281814090705 31.06155307765388 19.52597629881494 105-109 24.905 23.425 31.41 20.26 110-114 23.658121369917883 25.500700981373924 31.439014620468658 19.402163028239535 115-119 23.69724713112184 25.841851131874332 30.438326958048535 20.022574778955292 120-124 23.755713560182834 40.82021330624683 19.070594210259014 16.353478923311325 125-129 NaN NaN NaN NaN 130-134 23.61058838288797 25.705683101242 30.247145903901647 20.436582611968383 135-139 23.847966705109563 25.663139948854237 31.394474251617112 19.094419094419095 140-144 24.152249134948097 26.284995281535075 30.550487574709027 19.0122680088078 145-149 23.61632857500626 24.868519909842224 32.30653643876784 19.208615076383673 150-151 23.360040060090135 24.912368552829246 32.43615423134702 19.2914371557336 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 1.5 19 2.5 20 1.0 21 1.0 22 2.0 23 3.0 24 4.5 25 6.5 26 11.5 27 14.0 28 16.0 29 22.5 30 24.5 31 27.5 32 41.0 33 58.5 34 86.0 35 95.0 36 101.0 37 170.5 38 239.5 39 247.5 40 284.0 41 298.0 42 239.0 43 236.5 44 268.5 45 242.5 46 212.0 47 170.0 48 112.5 49 83.0 50 72.5 51 71.5 52 49.5 53 46.5 54 54.5 55 59.0 56 65.0 57 44.5 58 31.5 59 37.0 60 30.5 61 20.5 62 21.5 63 19.5 64 15.5 65 12.0 66 6.5 67 4.5 68 4.0 69 3.5 70 2.0 71 1.0 72 1.0 73 1.0 74 1.0 75 0.5 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.5 82 0.5 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 0.35000000000000003 2 0.27499999999999997 3 0.27499999999999997 4 0.27499999999999997 5 0.27499999999999997 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.03 40-44 0.06 45-49 0.295 50-54 0.20500000000000002 55-59 0.23500000000000001 60-64 0.16999999999999998 65-69 0.13999999999999999 70-74 0.145 75-79 0.02 80-84 0.1 85-89 0.03 90-94 0.0 95-99 0.0 100-104 0.005 105-109 0.0 110-114 0.13999999999999999 115-119 20.265 120-124 21.240000000000002 125-129 100.0 130-134 60.145 135-139 0.28500000000000003 140-144 20.525 145-149 80.035 150-151 0.15 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.1 #Duplication Level Percentage of deduplicated Percentage of total 1 82.5684380032206 51.275000000000006 2 6.964573268921096 8.649999999999999 3 3.059581320450886 5.7 4 2.1739130434782608 5.4 5 1.1674718196457328 3.6249999999999996 6 1.4492753623188406 5.4 7 0.36231884057971014 1.575 8 0.5636070853462157 2.8000000000000003 9 0.1610305958132045 0.8999999999999999 >10 1.529790660225443 14.674999999999999 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source ATTATCTATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAAT 36 0.8999999999999999 No Hit TATCTATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTT 35 0.8750000000000001 No Hit GTTATTTTGGCCGATTAATCTTCCAATATGCTAGTTTCAACAACTCTCGT 25 0.625 No Hit GCTTGTTATATGGGTCGTGAGTGGGAACTTAGTTTCCGTTTGGGTATGCG 23 0.575 No Hit GTGGGAACTTAGTTTCCGTTTGGGTATGCGCCCTTGGATTGCTGTTGCAT 23 0.575 No Hit GTTAGGTGTAGCTGGTGTTTTCGGCGGTTCCCTATTCAGTGCTATGCATG 22 0.5499999999999999 No Hit GCACAACATCCTTATGCATCCATTTCACATGTTAGGTGTAGCTGGTGTTT 21 0.525 No Hit CTATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAA 20 0.5 No Hit GGAATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCTAAA 19 0.475 No Hit CTTCCAATATGCTAGTTTCAACAACTCTCGTTCTTTACACTTCTTCTTGG 17 0.42500000000000004 No Hit GTTCGATCCTGGCTCAGGATGAACGCTGGCGGCATGCTTAACACATGCAA 16 0.4 No Hit GGAAGCTTCTCTGATGGTATGCCTTTAGGAATATCTGGTACTTTCAACTT 16 0.4 No Hit GGAAGAGACTTATAATATTGTGGCTGCTCATGGTTATTTTGGCCGATTAA 15 0.375 No Hit ATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAA 15 0.375 No Hit ATCTATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTG 15 0.375 No Hit CCTAAATGGATTCAATTTCAACCAATCTGTAGTTGATAGTCAAGGTCGCG 14 0.35000000000000003 No Hit CTCTAGTTTGATCAGGGAAACTACTGAAAATGAATCTGCTAATGAGGGTT 13 0.325 No Hit GTTTCAACAACTCTCGTTCTTTACACTTCTTCTTGGCTGCTTGGCCTGTA 13 0.325 No Hit GCTGCGACTGCTGTTTTCTTGATTTACCCTATTGGTCAAGGAAGCTTCTC 13 0.325 No Hit AGGGAAACTACTGAAAATGAATCTGCTAATGAGGGTTACAAATTTGGTCA 13 0.325 No Hit TCTATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGA 13 0.325 No Hit GGGAAACTACTGAAAATGAATCTGCTAATGAGGGTTACAAATTTGGTCAA 12 0.3 No Hit CCAAGATCTTACCATGACTGCAATTTTAGAGAGACGCGAAAGTACAAGCC 12 0.3 No Hit CTTATGAGCTAATTGTTCTACACTTCTTACTTGGTGTAGCTTGTTATATG 12 0.3 No Hit GCTAGTTTCAACAACTCTCGTTCTTTACACTTCTTCTTGGCTGCTTGGCC 12 0.3 No Hit CTAGCACTGAAAATCGTCTTTACATCGGATGGTTCGGTGTTTTGATGATC 12 0.3 No Hit GTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGATCTTACCATGAC 12 0.3 No Hit GGTCGCTTCTGCAACTGGATAACTAGCACTGAAAATCGTCTTTACATCGG 11 0.27499999999999997 No Hit GAGGGTTACAAATTTGGTCAAGAGGAAGAGACTTATAATATTGTGGCTGC 11 0.27499999999999997 No Hit GACTTATAATATTGTGGCTGCTCATGGTTATTTTGGCCGATTAATCTTCC 11 0.27499999999999997 No Hit GTTTTGATGATCCCTACCTTATTGACCGCAACTTCTGTATTTATTATCGC 11 0.27499999999999997 No Hit ATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCTAAATGG 11 0.27499999999999997 No Hit GTTGCATATTCAGCTCCTGTTGCAGCTGCGACTGCTGTTTTCTTGATTTA 11 0.27499999999999997 No Hit GTAGGAATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCT 11 0.27499999999999997 No Hit CTGAAAATGAATCTGCTAATGAGGGTTACAAATTTGGTCAAGAGGAAGAG 11 0.27499999999999997 No Hit CTTAGTTTCCGTTTGGGTATGCGCCCTTGGATTGCTGTTGCATATTCAGC 10 0.25 No Hit ATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCA 10 0.25 No Hit GTTCAACCTAAATGGATTCAATTTCAACCAATCTGTAGTTGATAGTCAAG 10 0.25 No Hit ATTCAGTGCTATGCATGGTTCCTTGGTAACCTCTAGTTTGATCAGGGAAA 9 0.22499999999999998 No Hit GAGGAAGAGACTTATAATATTGTGGCTGCTCATGGTTATTTTGGCCGATT 9 0.22499999999999998 No Hit CGAAAGTACAAGCCTGTGGGGTCGCTTCTGCAACTGGATAACTAGCACTG 9 0.22499999999999998 No Hit CTTTATGATTGTATTCCAGGCAGAGCACAACATCCTTATGCATCCATTTC 9 0.22499999999999998 No Hit CTATTCAGTGCTATGCATGGTTCCTTGGTAACCTCTAGTTTGATCAGGGA 8 0.2 No Hit ATCGGATTGCACTTTTACCCAATTTGGGAAGCTGCATCCGTTGATGAATG 8 0.2 No Hit GTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGATCTT 8 0.2 No Hit GAGACTTATAATATTGTGGCTGCTCATGGTTATTTTGGCCGATTAATCTT 8 0.2 No Hit CCTGTAGTAGGAATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTT 8 0.2 No Hit CGTTGATGAATGGTTATACAATGGTGGTCCTTATGAGCTAATTGTTCTAC 8 0.2 No Hit ATTTGGTCAAGAGGAAGAGACTTATAATATTGTGGCTGCTCATGGTTATT 8 0.2 No Hit CTTGCAATTTGAATAAACCAAGATCTTACCATGACTGCAATTTTAGAGAG 8 0.2 No Hit TGATGGTATTCGCGAGCCTGTTTCTGGTTCTTTACTTTATGGAAACAATA 8 0.2 No Hit CACATGTTAGGTGTAGCTGGTGTTTTCGGCGGTTCCCTATTCAGTGCTAT 8 0.2 No Hit GGATAACTAGCACTGAAAATCGTCTTTACATCGGATGGTTCGGTGTTTTG 8 0.2 No Hit GTTTGATCAGGGAAACTACTGAAAATGAATCTGCTAATGAGGGTTACAAA 8 0.2 No Hit GTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGATCTTACCATGACTG 8 0.2 No Hit CAACATCCTTATGCATCCATTTCACATGTTAGGTGTAGCTGGTGTTTTCG 8 0.2 No Hit GCTCATGGTTATTTTGGCCGATTAATCTTCCAATATGCTAGTTTCAACAA 7 0.17500000000000002 No Hit GTCGTGAGTGGGAACTTAGTTTCCGTTTGGGTATGCGCCCTTGGATTGCT 7 0.17500000000000002 No Hit GGAAACTACTGAAAATGAATCTGCTAATGAGGGTTACAAATTTGGTCAAG 7 0.17500000000000002 No Hit CTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCTAAATGGAT 7 0.17500000000000002 No Hit CCTATTCAGTGCTATGCATGGTTCCTTGGTAACCTCTAGTTTGATCAGGG 7 0.17500000000000002 No Hit GCCTTTAGGAATATCTGGTACTTTCAACTTTATGATTGTATTCCAGGCAG 7 0.17500000000000002 No Hit CTTCTTACTTGGTGTAGCTTGTTATATGGGTCGTGAGTGGGAACTTAGTT 7 0.17500000000000002 No Hit TTGTATTCCAGGCAGAGCACAACATCCTTATGCATCCATTTCACATGTTA 7 0.17500000000000002 No Hit GTAGATATTGATGGTATTCGCGAGCCTGTTTCTGGTTCTTTACTTTATGG 7 0.17500000000000002 No Hit AGCACAACATCCTTATGCATCCATTTCACATGTTAGGTGTAGCTGGTGTT 6 0.15 No Hit CATGGTTATTTTGGCCGATTAATCTTCCAATATGCTAGTTTCAACAACTC 6 0.15 No Hit GCTAATTGTTCTACACTTCTTACTTGGTGTAGCTTGTTATATGGGTCGTG 6 0.15 No Hit ATTTCAACCAATCTGTAGTTGATAGTCAAGGTCGCGTTATTAATACTTGG 6 0.15 No Hit TGTAGCTTGTTATATGGGTCGTGAGTGGGAACTTAGTTTCCGTTTGGGTA 6 0.15 No Hit GTACTATGGCGTTCAACCTAAATGGATTCAATTTCAACCAATCTGTAGTT 6 0.15 No Hit CTGCGACTGCTGTTTTCTTGATTTACCCTATTGGTCAAGGAAGCTTCTCT 6 0.15 No Hit CTTCTTCTTGGCTGCTTGGCCTGTAGTAGGAATCTGGTTCACTGCTTTAG 6 0.15 No Hit AGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGATCT 6 0.15 No Hit CTTGTTATATGGGTCGTGAGTGGGAACTTAGTTTCCGTTTGGGTATGCGC 6 0.15 No Hit AAACAATATTATCTCTGGTGCTATTATTCCTACTTCTGCGGCAATCGGAT 6 0.15 No Hit GCATGGTTCCTTGGTAACCTCTAGTTTGATCAGGGAAACTACTGAAAATG 6 0.15 No Hit GTGCTATGCATGGTTCCTTGGTAACCTCTAGTTTGATCAGGGAAACTACT 6 0.15 No Hit ATTTCACATGTTAGGTGTAGCTGGTGTTTTCGGCGGTTCCCTATTCAGTG 6 0.15 No Hit TTCCAATATGCTAGTTTCAACAACTCTCGTTCTTTACACTTCTTCTTGGC 6 0.15 No Hit GGCTTGCGGTGGATACCTAGGCACCCAGAGACGAGGAAGGGCGTAGCAAG 6 0.15 No Hit TATGCCTTTAGGAATATCTGGTACTTTCAACTTTATGATTGTATTCCAGG 6 0.15 No Hit GTAACCTCTAGTTTGATCAGGGAAACTACTGAAAATGAATCTGCTAATGA 6 0.15 No Hit ATCTTCCAATATGCTAGTTTCAACAACTCTCGTTCTTTACACTTCTTCTT 6 0.15 No Hit GTTATTAATACTTGGGCTGATATCATCAACCGTGCTAATCTTGGTATGGA 6 0.15 No Hit CGCGAAAGTACAAGCCTGTGGGGTCGCTTCTGCAACTGGATAACTAGCAC 6 0.15 No Hit CAGTGCTATGCATGGTTCCTTGGTAACCTCTAGTTTGATCAGGGAAACTA 6 0.15 No Hit TGTAGCTGGTGTTTTCGGCGGTTCCCTATTCAGTGCTATGCATGGTTCCT 6 0.15 No Hit CAATGGTGGTCCTTATGAGCTAATTGTTCTACACTTCTTACTTGGTGTAG 6 0.15 No Hit GTTGCAGCTGCGACTGCTGTTTTCTTGATTTACCCTATTGGTCAAGGAAG 6 0.15 No Hit CTTATTGACCGCAACTTCTGTATTTATTATCGCCTTCATCGCAGCCCCTC 6 0.15 No Hit CGTTATTAATACTTGGGCTGATATCATCAACCGTGCTAATCTTGGTATGG 6 0.15 No Hit GCTGCATCCGTTGATGAATGGTTATACAATGGTGGTCCTTATGAGCTAAT 6 0.15 No Hit TATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGATCTTACCAT 6 0.15 No Hit CAGGGAAACTACTGAAAATGAATCTGCTAATGAGGGTTACAAATTTGGTC 6 0.15 No Hit GAAGAGACTTATAATATTGTGGCTGCTCATGGTTATTTTGGCCGATTAAT 6 0.15 No Hit GGCCGATTAATCTTCCAATATGCTAGTTTCAACAACTCTCGTTCTTTACA 6 0.15 No Hit GAATGGTTATACAATGGTGGTCCTTATGAGCTAATTGTTCTACACTTCTT 6 0.15 No Hit GTTCTTTACTTTATGGAAACAATATTATCTCTGGTGCTATTATTCCTACT 6 0.15 No Hit TGATAGTCAAGGTCGCGTTATTAATACTTGGGCTGATATCATCAACCGTG 6 0.15 No Hit AGCTTGTTATATGGGTCGTGAGTGGGAACTTAGTTTCCGTTTGGGTATGC 6 0.15 No Hit TTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAA 5 0.125 No Hit GGTAACCTCTAGTTTGATCAGGGAAACTACTGAAAATGAATCTGCTAATG 5 0.125 No Hit CAACTCTCGTTCTTTACACTTCTTCTTGGCTGCTTGGCCTGTAGTAGGAA 5 0.125 No Hit TATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAA 5 0.125 No Hit TAGGAATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCTA 5 0.125 No Hit GTTCCTTGGTAACCTCTAGTTTGATCAGGGAAACTACTGAAAATGAATCT 5 0.125 No Hit CTTCTGTATTTATTATCGCCTTCATCGCAGCCCCTCCAGTAGATATTGAT 5 0.125 No Hit TTATAATATTGTGGCTGCTCATGGTTATTTTGGCCGATTAATCTTCCAAT 5 0.125 No Hit GGTTATTTTGGCCGATTAATCTTCCAATATGCTAGTTTCAACAACTCTCG 5 0.125 No Hit GAACTTAGTTTCCGTTTGGGTATGCGCCCTTGGATTGCTGTTGCATATTC 5 0.125 No Hit AGTTGATAGTCAAGGTCGCGTTATTAATACTTGGGCTGATATCATCAACC 5 0.125 No Hit GCATCCATTTCACATGTTAGGTGTAGCTGGTGTTTTCGGCGGTTCCCTAT 5 0.125 No Hit CTTTAGGAATATCTGGTACTTTCAACTTTATGATTGTATTCCAGGCAGAG 5 0.125 No Hit GATGAACGCTGGCGGCATGCTTAACACATGCAAGTCGAACGGGAAGTGGT 5 0.125 No Hit GTTACAAATTTGGTCAAGAGGAAGAGACTTATAATATTGTGGCTGCTCAT 5 0.125 No Hit CACAACATCCTTATGCATCCATTTCACATGTTAGGTGTAGCTGGTGTTTT 5 0.125 No Hit GTTATACAATGGTGGTCCTTATGAGCTAATTGTTCTACACTTCTTACTTG 5 0.125 No Hit AGTCAAGGTCGCGTTATTAATACTTGGGCTGATATCATCAACCGTGCTAA 5 0.125 No Hit CAACAACTCTCGTTCTTTACACTTCTTCTTGGCTGCTTGGCCTGTAGTAG 5 0.125 No Hit GTTTCTGGTTCTTTACTTTATGGAAACAATATTATCTCTGGTGCTATTAT 5 0.125 No Hit ATTGTATTCCAGGCAGAGCACAACATCCTTATGCATCCATTTCACATGTT 5 0.125 No Hit CGGATGGTTCGGTGTTTTGATGATCCCTACCTTATTGACCGCAACTTCTG 5 0.125 No Hit TGCAATTTGAATAAACCAAGATCTTACCATGACTGCAATTTTAGAGAGAC 5 0.125 No Hit TTTAGGAATATCTGGTACTTTCAACTTTATGATTGTATTCCAGGCAGAGC 5 0.125 No Hit GTACAAGCCTGTGGGGTCGCTTCTGCAACTGGATAACTAGCACTGAAAAT 5 0.125 No Hit CTCATGGTTATTTTGGCCGATTAATCTTCCAATATGCTAGTTTCAACAAC 5 0.125 No Hit GGTACTTTCAACTTTATGATTGTATTCCAGGCAGAGCACAACATCCTTAT 5 0.125 No Hit GAGCACAACATCCTTATGCATCCATTTCACATGTTAGGTGTAGCTGGTGT 5 0.125 No Hit ATTGATGGTATTCGCGAGCCTGTTTCTGGTTCTTTACTTTATGGAAACAA 5 0.125 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.037500000000000006 0.0 0.0 0.0 0.0 66-67 0.05 0.0 0.0 0.0 0.0 68-69 0.075 0.0 0.0 0.0 0.0 70-71 0.075 0.0 0.0 0.0 0.0 72-73 0.0875 0.0 0.0 0.0 0.0 74-75 0.1125 0.0 0.0 0.0 0.0 76-77 0.21250000000000002 0.0 0.0 0.0 0.0 78-79 0.275 0.0 0.0 0.0 0.0 80-81 0.3625 0.0 0.0 0.0 0.0 82-83 0.4375 0.0 0.0 0.0 0.0 84-85 0.525 0.0 0.0 0.0 0.0 86-87 0.6125 0.0 0.0 0.0 0.0 88-89 0.675 0.0 0.0 0.0 0.0 90-91 0.75 0.0 0.0 0.0 0.0 92-93 0.875 0.0 0.0 0.0 0.0 94-95 1.1124999999999998 0.0 0.0 0.0 0.0 96-97 1.325 0.0 0.0 0.0 0.0 98-99 1.4500000000000002 0.0 0.0 0.0 0.0 100-101 1.5875 0.0 0.0 0.0 0.0 102-103 1.775 0.0 0.0 0.0 0.0 104-105 2.0375 0.0 0.0 0.0 0.0 106-107 2.375 0.0 0.0 0.0 0.0 108-109 2.45 0.0 0.0 0.0 0.0 110-111 2.45 0.0 0.0 0.0 0.0 112-113 2.45 0.0 0.0 0.0 0.0 114-115 2.45 0.0 0.0 0.0 0.0 116-117 2.45 0.0 0.0 0.0 0.0 118-119 2.45 0.0 0.0 0.0 0.0 120-121 2.45 0.0 0.0 0.0 0.0 122-123 2.45 0.0 0.0 0.0 0.0 124-125 2.45 0.0 0.0 0.0 0.0 126-127 2.45 0.0 0.0 0.0 0.0 128-129 2.45 0.0 0.0 0.0 0.0 130-131 2.45 0.0 0.0 0.0 0.0 132-133 2.45 0.0 0.0 0.0 0.0 134-135 2.45 0.0 0.0 0.0 0.0 136-137 2.45 0.0 0.0 0.0 0.0 138-139 2.45 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content pass >>END_MODULE Read 1542731 spots for SRR6941579.sra Written 1542731 spots for SRR6941579.sra Read 1542731 spots for SRR6941579.sra Written 1542731 spots for SRR6941579.sra Read 1542731 spots for SRR6941579.sra Written 1542731 spots for SRR6941579.sra Read 1542731 spots for SRR6941579.sra Written 1542731 spots for SRR6941579.sra Read 1542731 spots for SRR6941579.sra Written 1542731 spots for SRR6941579.sra Read 1542731 spots for SRR6941579.sra Written 1542731 spots for SRR6941579.sra Read 1542731 spots for SRR6941579.sra Written 1542731 spots for SRR6941579.sra Read 1542731 spots for SRR6941579.sra Written 1542731 spots for SRR6941579.sra Read 1542731 spots for SRR6941579.sra Written 1542731 spots for SRR6941579.sra Read 1542731 spots for SRR6941579.sra Written 1542731 spots for SRR6941579.sra Read 1542731 spots for SRR6941579.sra Written 1542731 spots for SRR6941579.sra Read 1542731 spots for SRR6941579.sra Written 1542731 spots for SRR6941579.sra Read 1542731 spots for SRR6941579.sra Written 1542731 spots for SRR6941579.sra Read 1542747 spots for SRR6941579.sra Written 1542747 spots for SRR6941579.sra Read 1542731 spots for SRR6941579.sra Written 1542731 spots for SRR6941579.sra Read 1542731 spots for SRR6941579.sra Written 1542731 spots for SRR6941579.sra Read 1542731 spots for SRR6941579.sra Written 1542731 spots for SRR6941579.sra Read 1542731 spots for SRR6941579.sra Written 1542731 spots for SRR6941579.sra Read 1542731 spots for SRR6941579.sra Written 1542731 spots for SRR6941579.sra Read 1542731 spots for SRR6941579.sra Written 1542731 spots for SRR6941579.sra SRR ids: ['SRR6941579.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_dctt_v5q SRR6941579.sra spots: 30854636 blocks: [[1, 1542731], [1542732, 3085462], [3085463, 4628193], [4628194, 6170924], [6170925, 7713655], [7713656, 9256386], [9256387, 10799117], [10799118, 12341848], [12341849, 13884579], [13884580, 15427310], [15427311, 16970041], [16970042, 18512772], [18512773, 20055503], [20055504, 21598234], [21598235, 23140965], [23140966, 24683696], [24683697, 26226427], [26226428, 27769158], [27769159, 29311889], [29311890, 30854636]] SRR6941579 file size 10433923 SRR6941579 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR6941579 SRR6941579_1.fastq SRR6941579_2.fastq Input file: SRR6941579_1.fastq Paired file: SRR6941579_2.fastq trimmed: SRR6941579-trimmed-pair1.fastq, SRR6941579-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Fri Dec 6 12:00:50 2024 >> started Fri Dec 6 12:01:31 2024 >> done (41.544s) 30854636 read pairs processed; of these: 32813 ( 0.11%) short read pairs filtered out after trimming by size control 31250 ( 0.10%) empty read pairs filtered out after trimming by size control 30790573 (99.79%) read pairs available; of these: 24731220 (80.32%) trimmed read pairs available after processing 6059353 (19.68%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 3 0.00% 19 2 0.00% 20 4 0.00% 21 1 0.00% 22 4 0.00% 23 0 0.00% 24 7 0.00% 25 11 0.00% 26 10 0.00% 27 6 0.00% 28 7 0.00% 29 14 0.00% 30 18 0.00% 31 17 0.00% 32 17 0.00% 33 34 0.00% 34 28 0.00% 35 40 0.00% 36 35 0.00% 37 46 0.00% 38 39 0.00% 39 58 0.00% 40 78 0.00% 41 99 0.00% 42 93 0.00% 43 134 0.00% 44 137 0.00% 45 129 0.00% 46 166 0.00% 47 171 0.00% 48 231 0.00% 49 273 0.00% 50 294 0.00% 51 385 0.00% 52 456 0.00% 53 440 0.00% 54 518 0.00% 55 649 0.00% 56 662 0.00% 57 748 0.00% 58 870 0.00% 59 1004 0.00% 60 1067 0.00% 61 1145 0.00% 62 1604 0.01% 63 1758 0.01% 64 2078 0.01% 65 2378 0.01% 66 2526 0.01% 67 2732 0.01% 68 3086 0.01% 69 3704 0.01% 70 4011 0.01% 71 5006 0.02% 72 5743 0.02% 73 6091 0.02% 74 6429 0.02% 75 7940 0.03% 76 7513 0.02% 77 8779 0.03% 78 8662 0.03% 79 9526 0.03% 80 10652 0.03% 81 11852 0.04% 82 13567 0.04% 83 13927 0.05% 84 15439 0.05% 85 19373 0.06% 86 19780 0.06% 87 21036 0.07% 88 24515 0.08% 89 25077 0.08% 90 27639 0.09% 91 27647 0.09% 92 30217 0.10% 93 32108 0.10% 94 41633 0.14% 95 35505 0.12% 96 34840 0.11% 97 34643 0.11% 98 34624 0.11% 99 34741 0.11% 100 35755 0.12% 101 37955 0.12% 102 41477 0.13% 103 40604 0.13% 104 44253 0.14% 105 47419 0.15% 106 49459 0.16% 107 47889 0.16% 108 49194 0.16% 109 56662 0.18% 110 52224 0.17% 111 59765 0.19% 112 71241 0.23% 113 53586 0.17% 114 64899 0.21% 115 61085 0.20% 116 80163 0.26% 117 79972 0.26% 118 79975 0.26% 119 95353 0.31% 120 101222 0.33% 121 105135 0.34% 122 110599 0.36% 123 119182 0.39% 124 126975 0.41% 125 133399 0.43% 126 134201 0.44% 127 151021 0.49% 128 193831 0.63% 129 295420 0.96% 130 594728 1.93% 131 1296793 4.21% 132 901551 2.93% 133 4921174 15.98% 134 6287580 20.42% 135 73264 0.24% 136 56061 0.18% 137 57805 0.19% 138 72322 0.23% 139 118895 0.39% 140 634771 2.06% 141 1997297 6.49% 142 216272 0.70% 143 2438146 7.92% 144 109168 0.35% 145 755183 2.45% 146 39210 0.13% 147 47529 0.15% 148 63494 0.21% 149 101392 0.33% 150 720139 2.34% 151 6059353 19.68% 30790573 reads passed initial QC criterion=sequence-density sequence-density=0.38 sequence-density-rank=1 fanout-score=3.14 fanout-score-rank=22 prefix-density=0.44 prefix-fanout=2.7 sequence=TACTTGTTCAAC criterion=fanout-score sequence-density=0.01 sequence-density-rank=38 fanout-score=65.30 fanout-score-rank=1 prefix-density=0.20 prefix-fanout=4.3 sequence=ATAAAAAAAAGGGGGGGTAAGGACCCGCTAAGCTCCTACTTTTTCATGTTTCCAATCCGATCCCTCCGATTACTATAGAGATGAACCCAATCCAGAATATGAACCATAAAAGAAAACACCTACTAAACCAATCACAAGAATACCAGTTACCGTACCTATCAGCCAAAGAGGAATTCTTCCAGTAGTATCGGCCATTTCCCCTACTTTCCTCCACATTTTATCAAGTGGTCATGCTAGAGACAAAAACAGTCATGGATAGTTATGTTATAAGGATGGTATCCTTCCAAATGGGATAAGAGAGTTCTTACTACTCTCTTCTTTTCTCTCAATTAAAGAAGTAATTGGAAAACAAAACAGCAAGTACAAAAATGAGTAATAAACCCCAGTATAGACTGGTACGATTCAATTCAACATTTTGTTCATTCGGGTTTGATTGTGTCATAGTTCTATAGTTGGAATTTAGTTTATCGTTGGATGAACTGCATTGCTGATATTGATCCCAAGAAAAAAA criterion=sequence-density sequence-density=0.26 sequence-density-rank=1 fanout-score=1.95 fanout-score-rank=27 prefix-density=0.25 prefix-fanout=2.0 sequence=GTTGAACAAGTA criterion=fanout-score sequence-density=0.01 sequence-density-rank=32 fanout-score=30.63 fanout-score-rank=1 prefix-density=0.06 prefix-fanout=6.7 sequence=TTTTTTTTTATGAGATTTTTGCTAAAGTTTCATTTACGCCTAATTCACATCGAGTAGACCTTGTTATTGTGAGAATTCTTAATTCAAGAGTTGTAAGGAGGGACTTATGTCACCACAAACAGAAACTAAAGCAAGTGTTGGATTTAAAGCTGGTGTTAAAGATTATAGATTGACTTACTACACCCCGGAGTATGAAACCAAGGATACTGATATCTTGGCAGCATTCCGAGTATCTCCTCAACCTGGGGTTCCGCCCGAAGAAGCAGGGGCTGCAGTAGCTGCCGAATCTTCTACTGGTACATGGACAACTGTTTGGACTGATGGACTTACTAGTCTTGATCGTTACAAAGGACGATGCTATCACATCGAGCCTGTTCCTGGGGAAGACAGTCAATGGATCTGTTATGTAGCTTATCCATTAGATCTATTTGAAGAGGGTTCCGTTACTAACATGTTTACTTCCATTGTAGGTAACGTATTTGGTTTCAAAGCCCTACGTGCTCTACGTCTG SRR6941579 testing PE reads STAR mapping to Ensembl genome Started job on | Dec 06 12:02:22 Started mapping on | Dec 06 12:02:22 Finished on | Dec 06 12:05:09 Mapping speed, Million of reads per hour | 663.75 Number of input reads | 30790573 Average input read length | 274 UNIQUE READS: Uniquely mapped reads number | 19102397 Uniquely mapped reads % | 62.04% Average mapped length | 275.45 Number of splices: Total | 3117905 Number of splices: Annotated (sjdb) | 2805166 Number of splices: GT/AG | 2945860 Number of splices: GC/AG | 37621 Number of splices: AT/AC | 17460 Number of splices: Non-canonical | 116964 Mismatch rate per base, % | 0.36% Deletion rate per base | 0.01% Deletion average length | 1.42 Insertion rate per base | 0.01% Insertion average length | 1.99 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 10204001 % of reads mapped to multiple loci | 33.14% Number of reads mapped to too many loci | 35601 % of reads mapped to too many loci | 0.12% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 4.07% % of reads unmapped: other | 0.63% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 1503758 1503758 1503758 N_multimapping 10204001 10204001 10204001 N_noFeature 8209256 18350698 8555606 N_ambiguous 776514 6913 386900 UnstrandedReadsAssigned:10116627 PositiveStrandReadsAssigned:744786 NegativeStrandReadsAssigned:10159891 Dataset is classified negative stranded MeadianReadLen=151 20thPercentileLength=151 echo kmer=147 SRR6941579 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,972 [index] number of k-mers: 66,720,672 [index] number of equivalence classes: 111,837 [quant] running in paired-end mode [quant] will process pair 1: SRR6941579-trimmed-pair1.fastq SRR6941579-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 30,790,573 reads, 17,097,390 reads pseudoaligned [quant] estimated average fragment length: 239.424 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 998 rounds 52973 SRR6941579.ke.tsv 35125 SRR6941579.se.tsv 88098 total ==> SRR6941579.ke.tsv <== target_id length eff_length est_counts tpm PNS24245 936 698.241 0 0 PNS24247 1044 805.576 11.2451 0.752518 PNS24249 1928 1689.58 3.06455 0.0977797 PNS24246 1044 805.576 11.2451 0.752518 PNS24248 1044 805.576 11.2451 0.752518 PNS24244 1471 1232.58 39.2001 1.71449 PNS24243 293 101.443 0 0 KQK14069 1603 1364.58 3197.73 126.329 KQK14071 474 246.695 52.8001 11.5381 ==> SRR6941579.se.tsv <== BRADI_1g14170v3 4504 BRADI_1g53295v3 16 BRADI_1g59795v3 122 BRADI_1g07683v3 0 BRADI_1g00485v3 0 BRADI_1g20270v3 42 BRADI_1g74790v3 14 BRADI_1g09890v3 0 BRADI_1g77505v3 75 BRADI_1g48960v3 0 SRR6941579 completed mapping pipeline successfully