Starting /dee2/code/volunteer_pipeline.sh SRR6941584 current disk space = 1551419879424 free memory = 1604063700 SRR6941584 SRAfilesize 7b0e522d5c1a6e8009491ab070b574b6 SRR6941584.sra SRR6941584.sra file validated SRR6941584 is paired end SRR6941584 is conventional basespace SRR6941584 read1 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR6941584_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.1505 33.0 33.0 34.0 31.0 34.0 2 32.678 33.0 33.0 34.0 31.0 34.0 3 32.936 34.0 33.0 34.0 32.0 34.0 4 33.2495 34.0 33.0 34.0 32.0 34.0 5 33.3875 34.0 33.0 34.0 33.0 34.0 6 37.25775 38.0 38.0 38.0 36.0 38.0 7 37.56075 38.0 38.0 38.0 37.0 38.0 8 37.54675 38.0 38.0 38.0 38.0 38.0 9 37.5935 38.0 38.0 38.0 38.0 38.0 10-14 37.613749999999996 38.0 38.0 38.0 38.0 38.0 15-19 37.602850000000004 38.0 38.0 38.0 38.0 38.0 20-24 37.5912 38.0 38.0 38.0 38.0 38.0 25-29 37.5609 38.0 38.0 38.0 38.0 38.0 30-34 37.44065 38.0 38.0 38.0 38.0 38.0 35-39 37.510149999999996 38.0 38.0 38.0 38.0 38.0 40-44 37.48065 38.0 38.0 38.0 38.0 38.0 45-49 37.4578 38.0 38.0 38.0 37.6 38.0 50-54 37.532349999999994 38.0 38.0 38.0 38.0 38.0 55-59 37.460950000000004 38.0 38.0 38.0 37.8 38.0 60-64 37.436 38.0 38.0 38.0 37.6 38.0 65-69 37.36765 38.0 38.0 38.0 37.0 38.0 70-74 37.237100000000005 38.0 38.0 38.0 37.0 38.0 75-79 37.298700000000004 38.0 38.0 38.0 36.8 38.0 80-84 37.23175 38.0 38.0 38.0 36.8 38.0 85-89 37.17399999999999 38.0 38.0 38.0 36.6 38.0 90-94 37.1538 38.0 38.0 38.0 36.4 38.0 95-99 37.09825000000001 38.0 38.0 38.0 36.0 38.0 100-104 37.04755 38.0 38.0 38.0 36.0 38.0 105-109 36.95910000000001 38.0 38.0 38.0 35.6 38.0 110-114 36.58515 38.0 38.0 38.0 34.6 38.0 115-119 36.51595 38.0 38.0 38.0 34.4 38.0 120-124 36.641450000000006 38.0 38.0 38.0 34.4 38.0 125-129 36.49595000000001 38.0 38.0 38.0 34.4 38.0 130-134 36.35065 38.0 38.0 38.0 34.0 38.0 135-139 36.19545000000001 38.0 37.8 38.0 33.8 38.0 140-144 36.025749999999995 38.0 38.0 38.0 33.2 38.0 145-149 35.64399999999999 38.0 37.0 38.0 31.8 38.0 150-151 31.952875 35.5 32.0 38.0 16.5 38.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 11 1.0 12 1.0 13 0.0 14 1.0 15 1.0 16 0.0 17 0.0 18 0.0 19 1.0 20 2.0 21 3.0 22 3.0 23 5.0 24 6.0 25 2.0 26 10.0 27 10.0 28 17.0 29 25.0 30 29.0 31 32.0 32 51.0 33 66.0 34 95.0 35 187.0 36 427.0 37 3025.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 45.03957783641161 13.24538258575198 6.701846965699208 35.0131926121372 2 25.74430823117338 15.736802601951464 29.39704778583938 29.12184138103578 3 21.85 21.75 26.05 30.349999999999998 4 25.0 29.7 22.475 22.825 5 22.75 35.699999999999996 22.575 18.975 6 17.9 40.175 22.775000000000002 19.15 7 12.65 31.424999999999997 41.025 14.899999999999999 8 16.25 28.975 30.9 23.875 9 16.55 25.275 34.699999999999996 23.474999999999998 10-14 19.775000000000002 33.805 23.849999999999998 22.57 15-19 20.455000000000002 30.725 25.915 22.905 20-24 19.035 31.905 26.045 23.015 25-29 21.67 31.574999999999996 25.505 21.25 30-34 21.759999999999998 32.71 24.65 20.880000000000003 35-39 21.11 31.64 25.365 21.884999999999998 40-44 19.215 30.78 27.02 22.985 45-49 20.00700070007001 30.598059805980597 27.302730273027304 22.092209220922093 50-54 21.04 31.175000000000004 25.369999999999997 22.415 55-59 20.855 30.005 25.405 23.735 60-64 19.29 31.424999999999997 26.279999999999998 23.005 65-69 20.175 30.955 24.435000000000002 24.435000000000002 70-74 21.195 31.324999999999996 23.599999999999998 23.880000000000003 75-79 20.735 30.490000000000002 26.56 22.215 80-84 22.145 30.305 24.94 22.61 85-89 21.785 29.625 26.005 22.585 90-94 19.545 30.880000000000003 25.835 23.74 95-99 20.96 30.625000000000004 24.92 23.494999999999997 100-104 20.053021208483393 32.12284913965586 25.050020008003198 22.774109643857543 105-109 20.330000000000002 30.320000000000004 25.83 23.52 110-114 20.38888609757323 30.417524996231727 25.408229915088178 23.785358991106868 115-119 20.665261890427452 31.331527192454345 24.46317479430062 23.54003612281758 120-124 19.634999999999998 31.369999999999997 22.905 26.090000000000003 125-129 21.02 31.630000000000003 23.995 23.355 130-134 21.915000000000003 31.979999999999997 22.53 23.575 135-139 22.705000000000002 30.86 24.25 22.185 140-144 22.015 31.145 24.529999999999998 22.31 145-149 21.55 30.214999999999996 23.805 24.43 150-151 19.675 32.25 23.075000000000003 25.0 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.5 5 0.5 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.5 15 0.5 16 0.0 17 0.0 18 1.5 19 2.0 20 1.5 21 2.5 22 3.5 23 5.0 24 7.0 25 8.5 26 9.0 27 9.5 28 16.5 29 21.5 30 22.0 31 27.0 32 31.0 33 37.0 34 48.0 35 55.5 36 100.0 37 222.5 38 265.5 39 225.0 40 266.0 41 298.5 42 252.0 43 246.0 44 254.5 45 226.0 46 169.5 47 130.5 48 139.5 49 110.0 50 75.5 51 69.5 52 49.0 53 39.0 54 56.0 55 74.0 56 69.5 57 50.5 58 41.5 59 41.5 60 40.0 61 28.0 62 18.0 63 15.0 64 18.5 65 20.5 66 13.0 67 9.0 68 10.5 69 9.0 70 6.5 71 5.5 72 5.5 73 3.5 74 3.0 75 3.5 76 3.5 77 2.0 78 1.0 79 1.0 80 0.5 81 0.5 82 0.5 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content warn #Base N-Count 1 5.25 2 0.075 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.01 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.04 105-109 0.0 110-114 0.485 115-119 0.33999999999999997 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 66.95 #Duplication Level Percentage of deduplicated Percentage of total 1 86.14637789395071 57.675 2 5.974607916355489 8.0 3 2.912621359223301 5.8500000000000005 4 1.2696041822255415 3.4000000000000004 5 0.7468259895444361 2.5 6 0.5601194921583271 2.25 7 0.5601194921583271 2.625 8 0.4107542942494399 2.1999999999999997 9 0.18670649738610903 1.125 >10 1.194921583271098 12.9 >50 0.03734129947722181 1.4749999999999999 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCATTAAT 59 1.4749999999999999 No Hit GCGGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTACG 35 0.8750000000000001 No Hit GGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTACGTT 33 0.8250000000000001 No Hit CTTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCAT 32 0.8 No Hit GGCCAAAATAACCATGAGCAGCCACAATATTATAAGTCTCTTCCTCTTGA 31 0.775 No Hit GCCAAAATAACCATGAGCAGCCACAATATTATAAGTCTCTTCCTCTTGAC 31 0.775 No Hit GCTTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCA 20 0.5 No Hit GTTGAAACTAGCATATTGGAAGATTAATCGGCCAAAATAACCATGAGCAG 20 0.5 No Hit GCCGAAAACACCAGCTACACCTAACATGTGAAATGGATGCATAAGGATGT 19 0.475 No Hit CTCATAAGGACCACCATTGTATAACCATTCATCAACGGATGCAGCTTCCC 19 0.475 No Hit GCGGAAAAACCTTATCCATTAATAGCGGGAACTTCAAGAGCAGCTAGATC 18 0.44999999999999996 No Hit GTGCTCTGCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTCCTA 15 0.375 No Hit CGGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTACGT 14 0.35000000000000003 No Hit CCATATAACAAGCTACACCAAGTAAGAAGTGTAGAACAATTAGCTCATAA 14 0.35000000000000003 No Hit CATATAACAAGCTACACCAAGTAAGAAGTGTAGAACAATTAGCTCATAAG 14 0.35000000000000003 No Hit GCATAAGGATGTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAAAGTA 14 0.35000000000000003 No Hit GGTAAATCAAGAAAACAGCAGTCGCAGCTGCAACAGGAGCTGAATATGCA 14 0.35000000000000003 No Hit GCTGCGATGAAGGCGATAATAAATACAGAAGTTGCGGTCAATAAGGTAGG 13 0.325 No Hit GGCGCATACCCAAACGGAAACTAAGTTCCCACTCACGACCCATATAACAA 12 0.3 No Hit TTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCATTAATA 12 0.3 No Hit GGTCAATAAGGTAGGGATCATCAAAACACCGAACCATCCGATGTAAAGAC 12 0.3 No Hit AGCGGAAAAACCTTATCCATTAATAGCGGGAACTTCAAGAGCAGCTAGAT 12 0.3 No Hit CCCAAGTATTAATAACGCGACCTTGACTATCAACTACAGATTGGTTGAAA 11 0.27499999999999997 No Hit CTCGCGAATACCATCAATATCTACTGGAGGGGCTGCGATGAAGGCGATAA 11 0.27499999999999997 No Hit CGCAGAAGTAGGAATAATAGCACCAGAGATAATATTGTTTCCATAAAGTA 10 0.25 No Hit GCGATAATAAATACAGAAGTTGCGGTCAATAAGGTAGGGATCATCAAAAC 10 0.25 No Hit CCCATATAACAAGCTACACCAAGTAAGAAGTGTAGAACAATTAGCTCATA 10 0.25 No Hit GCTGAATATGCAACAGCAATCCAAGGGCGCATACCCAAACGGAAACTAAG 10 0.25 No Hit GCACTGAATAGGGAACCGCCGAAAACACCAGCTACACCTAACATGTGAAA 10 0.25 No Hit GTGAGCATTACGTTCGTGCATTACTTCCATACCAAGATTAGCACGGTTGA 10 0.25 No Hit CATCAGAGAAGCTTCCTTGACCAATAGGGTAAATCAAGAAAACAGCAGTC 10 0.25 No Hit GCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTCCTAAAGGCAT 10 0.25 No Hit GGGGCTGCGATGAAGGCGATAATAAATACAGAAGTTGCGGTCAATAAGGT 10 0.25 No Hit TTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCATT 9 0.22499999999999998 No Hit TGATGATATCAGCCCAAGTATTAATAACGCGACCTTGACTATCAACTACA 9 0.22499999999999998 No Hit CCACCATTGTATAACCATTCATCAACGGATGCAGCTTCCCAAATTGGGTA 9 0.22499999999999998 No Hit GTGCTAGTTATCCAGTTGCAGAAGCGACCCCACAGGCTTGTACTTTCGCG 9 0.22499999999999998 No Hit CTCTTGACCAAATTTGTAACCCTCATTAGCAGATTCATTTTCAGTAGTTT 9 0.22499999999999998 No Hit GCACGGTTGATGATATCAGCCCAAGTATTAATAACGCGACCTTGACTATC 8 0.2 No Hit CCGCAGAAGTAGGAATAATAGCACCAGAGATAATATTGTTTCCATAAAGT 8 0.2 No Hit GGAGGGGCTGCGATGAAGGCGATAATAAATACAGAAGTTGCGGTCAATAA 8 0.2 No Hit GGAAGATTAATCGGCCAAAATAACCATGAGCAGCCACAATATTATAAGTC 8 0.2 No Hit GGAAAAACCTTATCCATTAATAGCGGGAACTTCAAGAGCAGCTAGATCTA 8 0.2 No Hit ACCAGATATTCCTAAAGGCATACCATCAGAGAAGCTTCCTTGACCAATAG 8 0.2 No Hit CAAGTATTAATAACGCGACCTTGACTATCAACTACAGATTGGTTGAAATT 8 0.2 No Hit TTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCATTA 8 0.2 No Hit GCATATTGGAAGATTAATCGGCCAAAATAACCATGAGCAGCCACAATATT 8 0.2 No Hit GGATGCATAAGGATGTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAA 8 0.2 No Hit GCGACCTTGACTATCAACTACAGATTGGTTGAAATTGAATCCATTTAGGT 8 0.2 No Hit GCGGTCAATAAGGTAGGGATCATCAAAACACCGAACCATCCGATGTAAAG 7 0.17500000000000002 No Hit ACCAGCTACACCTAACATGTGAAATGGATGCATAAGGATGTTGTGCTCTG 7 0.17500000000000002 No Hit CTTGACTATCAACTACAGATTGGTTGAAATTGAATCCATTTAGGTTGAAC 7 0.17500000000000002 No Hit GCAGAAGTAGGAATAATAGCACCAGAGATAATATTGTTTCCATAAAGTAA 7 0.17500000000000002 No Hit GGGAAGTTGTGAGCATTACGTTCGTGCATTACTTCCATACCAAGATTAGC 7 0.17500000000000002 No Hit GGCATACCATCAGAGAAGCTTCCTTGACCAATAGGGTAAATCAAGAAAAC 7 0.17500000000000002 No Hit GCTAGTTATCCAGTTGCAGAAGCGACCCCACAGGCTTGTACTTTCGCGTC 7 0.17500000000000002 No Hit GTAAAGAACGAGAGTTGTTGAAACTAGCATATTGGAAGATTAATCGGCCA 7 0.17500000000000002 No Hit GTTAGCGGAAAAACCTTATCCATTAATAGCGGGAACTTCAAGAGCAGCTA 7 0.17500000000000002 No Hit CAGAGATAATATTGTTTCCATAAAGTAAAGAACCAGAAACAGGCTCGCGA 7 0.17500000000000002 No Hit GATGCATAAGGATGTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAAA 7 0.17500000000000002 No Hit CAGCAGTCGCAGCTGCAACAGGAGCTGAATATGCAACAGCAATCCAAGGG 7 0.17500000000000002 No Hit CACCATTGTATAACCATTCATCAACGGATGCAGCTTCCCAAATTGGGTAA 7 0.17500000000000002 No Hit GACCCATATAACAAGCTACACCAAGTAAGAAGTGTAGAACAATTAGCTCA 7 0.17500000000000002 No Hit CCAGATATTCCTAAAGGCATACCATCAGAGAAGCTTCCTTGACCAATAGG 7 0.17500000000000002 No Hit AGAGAAGCTTCCTTGACCAATAGGGTAAATCAAGAAAACAGCAGTCGCAG 6 0.15 No Hit AAAAATTCTTATATGTTAGCGGAAAAACCTTATCCATTAATAGCGGGAAC 6 0.15 No Hit GTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTC 6 0.15 No Hit GTCGCAGCTGCAACAGGAGCTGAATATGCAACAGCAATCCAAGGGCGCAT 6 0.15 No Hit GGCGATAATAAATACAGAAGTTGCGGTCAATAAGGTAGGGATCATCAAAA 6 0.15 No Hit ATCCGATTGCCGCAGAAGTAGGAATAATAGCACCAGAGATAATATTGTTT 6 0.15 No Hit CCAGATTCCTACTACAGGCCAAGCAGCCAAGAAGAAGTGTAAAGAACGAG 6 0.15 No Hit AGAGATAATATTGTTTCCATAAAGTAAAGAACCAGAAACAGGCTCGCGAA 6 0.15 No Hit CCAAGTATTAATAACGCGACCTTGACTATCAACTACAGATTGGTTGAAAT 6 0.15 No Hit GGCCAAGCAGCCAAGAAGAAGTGTAAAGAACGAGAGTTGTTGAAACTAGC 6 0.15 No Hit CATAAGGATGTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAAAGTAC 6 0.15 No Hit GTGAAATGGATGCATAAGGATGTTGTGCTCTGCCTGGAATACAATCATAA 6 0.15 No Hit GGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTACGTTC 6 0.15 No Hit GGACCACCATTGTATAACCATTCATCAACGGATGCAGCTTCCCAAATTGG 6 0.15 No Hit GCTCTGCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTCCTAAA 6 0.15 No Hit CTGCGATGAAGGCGATAATAAATACAGAAGTTGCGGTCAATAAGGTAGGG 5 0.125 No Hit CAGAGAAGCTTCCTTGACCAATAGGGTAAATCAAGAAAACAGCAGTCGCA 5 0.125 No Hit GTAAAGACGATTTTCAGTGCTAGTTATCCAGTTGCAGAAGCGACCCCACA 5 0.125 No Hit CCTACTACAGGCCAAGCAGCCAAGAAGAAGTGTAAAGAACGAGAGTTGTT 5 0.125 No Hit CCAAGAAGAAGTGTAAAGAACGAGAGTTGTTGAAACTAGCATATTGGAAG 5 0.125 No Hit GATGATATCAGCCCAAGTATTAATAACGCGACCTTGACTATCAACTACAG 5 0.125 No Hit ATCCATTAATAGCGGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTG 5 0.125 No Hit CTAACATGTGAAATGGATGCATAAGGATGTTGTGCTCTGCCTGGAATACA 5 0.125 No Hit GGATGTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAAAGTACCAGAT 5 0.125 No Hit GGAAGTTGTGAGCATTACGTTCGTGCATTACTTCCATACCAAGATTAGCA 5 0.125 No Hit GTTCGCTGGAGTAGGCTGCGAGGCCCAAGCGGCCAAGGAGTGCAGCGGCC 5 0.125 No Hit CAGATTCCTACTACAGGCCAAGCAGCCAAGAAGAAGTGTAAAGAACGAGA 5 0.125 No Hit ACCAAGATTAGCACGGTTGATGATATCAGCCCAAGTATTAATAACGCGAC 5 0.125 No Hit GAGCTGAATATGCAACAGCAATCCAAGGGCGCATACCCAAACGGAAACTA 5 0.125 No Hit AGCTGAATATGCAACAGCAATCCAAGGGCGCATACCCAAACGGAAACTAA 5 0.125 No Hit GCTAGATCTAGAGGGAAGTTGTGAGCATTACGTTCGTGCATTACTTCCAT 5 0.125 No Hit CATTAATAGCGGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGA 5 0.125 No Hit CTCTGCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTCCTAAAG 5 0.125 No Hit CGCGACCTTGACTATCAACTACAGATTGGTTGAAATTGAATCCATTTAGG 5 0.125 No Hit GCCAAGAAGAAGTGTAAAGAACGAGAGTTGTTGAAACTAGCATATTGGAA 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.025 0.0 0.0 0.0 0.0 50-51 0.025 0.0 0.0 0.0 0.0 52-53 0.025 0.0 0.0 0.0 0.0 54-55 0.025 0.0 0.0 0.0 0.0 56-57 0.025 0.0 0.0 0.0 0.0 58-59 0.025 0.0 0.0 0.0 0.0 60-61 0.037500000000000006 0.0 0.0 0.0 0.0 62-63 0.05 0.0 0.0 0.0 0.0 64-65 0.05 0.0 0.0 0.0 0.0 66-67 0.05 0.0 0.0 0.0 0.0 68-69 0.1 0.0 0.0 0.0 0.0 70-71 0.125 0.0 0.0 0.0 0.0 72-73 0.175 0.0 0.0 0.0 0.0 74-75 0.2 0.0 0.0 0.0 0.0 76-77 0.21250000000000002 0.0 0.0 0.0 0.0 78-79 0.25 0.0 0.0 0.0 0.0 80-81 0.275 0.0 0.0 0.0 0.0 82-83 0.32499999999999996 0.0 0.0 0.0 0.0 84-85 0.4 0.0 0.0 0.0 0.0 86-87 0.5 0.0 0.0 0.0 0.0 88-89 0.6125 0.0 0.0 0.0 0.0 90-91 0.7625 0.0 0.0 0.0 0.0 92-93 0.95 0.0 0.0 0.0 0.0 94-95 1.1375000000000002 0.0 0.0 0.0 0.0 96-97 1.275 0.0 0.0 0.0 0.0 98-99 1.475 0.0 0.0 0.0 0.0 100-101 1.7125 0.0 0.0 0.0 0.0 102-103 1.85 0.0 0.0 0.0 0.0 104-105 2.0999999999999996 0.0 0.0 0.0 0.0 106-107 2.4375 0.0 0.0 0.0 0.0 108-109 2.725 0.0 0.0 0.0 0.0 110-111 3.0625 0.0 0.0 0.0 0.0 112-113 3.3625 0.0 0.0 0.0 0.0 114-115 3.825 0.0 0.0 0.0 0.0 116-117 4.2125 0.0 0.0 0.0 0.0 118-119 4.75 0.0 0.0 0.0 0.0 120-121 5.3125 0.0 0.0 0.0 0.0 122-123 5.9375 0.0 0.0 0.0 0.0 124-125 6.625 0.0 0.0 0.0 0.0 126-127 7.6375 0.0 0.0 0.0 0.0 128-129 8.375 0.0 0.0 0.0 0.0 130-131 8.975 0.0 0.0 0.0 0.0 132-133 9.85 0.0 0.0 0.0 0.0 134-135 10.4125 0.0 0.0 0.0 0.0 136-137 11.1125 0.0 0.0 0.0 0.0 138-139 11.662500000000001 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAAATCC 10 0.0068537686 144.8375 7 >>END_MODULE SRR6941584 read2 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR6941584_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 33.0755 34.0 33.0 34.0 32.0 34.0 2 33.2065 34.0 33.0 34.0 33.0 34.0 3 33.25575 34.0 33.0 34.0 33.0 34.0 4 33.2075 34.0 33.0 34.0 33.0 34.0 5 33.208 34.0 33.0 34.0 33.0 34.0 6 37.3875 38.0 38.0 38.0 38.0 38.0 7 37.43425 38.0 38.0 38.0 38.0 38.0 8 37.326 38.0 38.0 38.0 38.0 38.0 9 37.38525 38.0 38.0 38.0 38.0 38.0 10-14 37.33325 38.0 38.0 38.0 37.6 38.0 15-19 37.32615 38.0 38.0 38.0 37.8 38.0 20-24 37.31095 38.0 38.0 38.0 37.2 38.0 25-29 37.223 38.0 38.0 38.0 37.0 38.0 30-34 37.252250000000004 38.0 38.0 38.0 37.4 38.0 35-39 37.2425 38.0 38.0 38.0 37.0 38.0 40-44 37.192 38.0 38.0 38.0 37.0 38.0 45-49 37.2145 38.0 38.0 38.0 37.0 38.0 50-54 37.20345 38.0 38.0 38.0 37.0 38.0 55-59 37.137249999999995 38.0 38.0 38.0 37.0 38.0 60-64 37.021249999999995 38.0 38.0 38.0 36.4 38.0 65-69 36.9559 38.0 38.0 38.0 36.6 38.0 70-74 36.96985 38.0 38.0 38.0 36.0 38.0 75-79 36.956799999999994 38.0 38.0 38.0 36.2 38.0 80-84 36.93725 38.0 38.0 38.0 36.0 38.0 85-89 36.8916 38.0 38.0 38.0 36.0 38.0 90-94 36.82625 38.0 38.0 38.0 35.8 38.0 95-99 36.680150000000005 38.0 38.0 38.0 35.0 38.0 100-104 36.50515 38.0 38.0 38.0 34.6 38.0 105-109 36.342200000000005 38.0 38.0 38.0 33.8 38.0 110-114 35.897800000000004 38.0 37.4 38.0 32.2 38.0 115-119 35.81845 38.0 37.4 38.0 32.2 38.0 120-124 35.75744999999999 38.0 37.0 38.0 31.8 38.0 125-129 35.72455 38.0 36.8 38.0 32.2 38.0 130-134 35.6203 38.0 36.4 38.0 31.4 38.0 135-139 35.23535 38.0 36.0 38.0 31.0 38.0 140-144 34.9228 38.0 36.0 38.0 30.2 38.0 145-149 33.88125 38.0 34.4 38.0 24.6 38.0 150-151 28.427374999999998 34.5 17.5 38.0 2.0 38.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6.0 3 1.0 4 3.0 5 2.0 6 1.0 7 0.0 8 0.0 9 3.0 10 0.0 11 2.0 12 0.0 13 3.0 14 3.0 15 0.0 16 1.0 17 0.0 18 3.0 19 2.0 20 6.0 21 8.0 22 4.0 23 8.0 24 16.0 25 10.0 26 9.0 27 21.0 28 22.0 29 35.0 30 36.0 31 43.0 32 74.0 33 81.0 34 117.0 35 204.0 36 523.0 37 2753.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.949999999999996 21.15 15.2 22.7 2 26.700000000000003 19.125 36.775000000000006 17.4 3 19.900000000000002 23.75 38.875 17.474999999999998 4 24.05 30.575000000000003 26.35 19.025 5 23.849999999999998 32.75 25.825 17.575 6 20.474999999999998 34.175 27.675 17.675 7 18.125 20.5 43.75 17.625 8 20.474999999999998 23.0 31.4 25.124999999999996 9 21.05 21.349999999999998 34.775 22.825 10-14 23.995 25.319999999999997 31.4 19.285 15-19 22.939999999999998 24.585 31.935000000000002 20.54 20-24 23.605 24.265 32.42 19.71 25-29 23.41 25.169999999999998 30.825000000000003 20.595 30-34 24.099999999999998 24.535 31.31 20.055 35-39 23.425 25.185000000000002 30.625000000000004 20.765 40-44 23.7 25.47 30.485 20.345 45-49 22.759999999999998 26.83 30.085 20.325 50-54 22.99 25.19 31.055 20.765 55-59 22.46 25.790000000000003 30.61 21.14 60-64 22.900000000000002 24.81 31.535000000000004 20.755000000000003 65-69 23.555 25.55 30.165 20.73 70-74 23.794999999999998 25.405 30.04 20.76 75-79 23.05 24.935 30.48 21.535 80-84 23.98 24.755 32.01 19.255 85-89 24.4 25.86 29.189999999999998 20.549999999999997 90-94 23.155 25.45 30.095 21.3 95-99 23.24 25.31 30.599999999999998 20.849999999999998 100-104 24.66 24.775 30.575000000000003 19.99 105-109 25.492549254925496 24.287428742874287 30.073007300730076 20.147014701470148 110-114 23.43117155857793 24.70123506175309 31.201560078003897 20.66603330166508 115-119 23.919999999999998 25.005 30.520000000000003 20.555 120-124 23.669999999999998 25.97 29.054999999999996 21.305 125-129 24.09 25.885 29.104999999999997 20.919999999999998 130-134 24.18 26.415 29.29 20.115 135-139 24.735 25.835 30.28 19.15 140-144 25.081254062703135 25.51127556377819 30.011500575028748 19.395969798489922 145-149 25.00500100020004 24.954990998199637 30.141028205641128 19.898979795959193 150-151 24.334125296986368 24.97186444916844 31.561835688383145 19.132174565462048 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.5 12 1.0 13 0.5 14 0.0 15 0.0 16 0.5 17 0.5 18 0.5 19 2.0 20 2.0 21 1.0 22 1.5 23 3.5 24 7.5 25 9.0 26 10.0 27 20.0 28 24.0 29 22.0 30 27.5 31 34.0 32 37.5 33 44.5 34 64.0 35 81.0 36 104.5 37 157.0 38 206.0 39 227.0 40 253.0 41 248.5 42 224.0 43 253.5 44 263.5 45 228.0 46 205.5 47 174.5 48 132.0 49 88.5 50 70.0 51 72.0 52 56.5 53 49.5 54 65.5 55 75.0 56 61.5 57 46.5 58 41.5 59 44.0 60 44.0 61 35.0 62 29.5 63 24.0 64 16.5 65 15.0 66 10.0 67 11.5 68 15.5 69 11.5 70 10.0 71 9.0 72 5.0 73 5.0 74 5.5 75 4.0 76 3.0 77 1.5 78 1.0 79 0.5 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.5 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.01 110-114 0.005 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.005 145-149 0.02 150-151 0.0375 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 65.525 #Duplication Level Percentage of deduplicated Percentage of total 1 83.51774132010684 54.725 2 6.982067913010301 9.15 3 2.9378099961846624 5.775 4 2.327355971003434 6.1 5 0.8393742846241892 2.75 6 0.9156810377718427 3.5999999999999996 7 0.6104540251812286 2.8000000000000003 8 0.2670736360167875 1.4000000000000001 9 0.3052270125906143 1.7999999999999998 >10 1.2972148035101108 11.899999999999999 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source ATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAA 26 0.65 No Hit GTGGGAACTTAGTTTCCGTTTGGGTATGCGCCCTTGGATTGCTGTTGCAT 24 0.6 No Hit GCTTGTTATATGGGTCGTGAGTGGGAACTTAGTTTCCGTTTGGGTATGCG 18 0.44999999999999996 No Hit GGAAGCTTCTCTGATGGTATGCCTTTAGGAATATCTGGTACTTTCAACTT 18 0.44999999999999996 No Hit ATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCA 18 0.44999999999999996 No Hit GTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGATCTT 17 0.42500000000000004 No Hit GCACAACATCCTTATGCATCCATTTCACATGTTAGGTGTAGCTGGTGTTT 17 0.42500000000000004 No Hit GTTCAACCTAAATGGATTCAATTTCAACCAATCTGTAGTTGATAGTCAAG 17 0.42500000000000004 No Hit TATCTATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTT 16 0.4 No Hit GTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGATCTTACCATGAC 16 0.4 No Hit GTTATTTTGGCCGATTAATCTTCCAATATGCTAGTTTCAACAACTCTCGT 15 0.375 No Hit GCTGCGACTGCTGTTTTCTTGATTTACCCTATTGGTCAAGGAAGCTTCTC 15 0.375 No Hit GCTAGTTTCAACAACTCTCGTTCTTTACACTTCTTCTTGGCTGCTTGGCC 14 0.35000000000000003 No Hit GCATGGTTCCTTGGTAACCTCTAGTTTGATCAGGGAAACTACTGAAAATG 14 0.35000000000000003 No Hit GTCAAGGTCGCGTTATTAATACTTGGGCTGATATCATCAACCGTGCTAAT 14 0.35000000000000003 No Hit ATTATCTATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAAT 14 0.35000000000000003 No Hit AGCACAACATCCTTATGCATCCATTTCACATGTTAGGTGTAGCTGGTGTT 13 0.325 No Hit ATCGGATTGCACTTTTACCCAATTTGGGAAGCTGCATCCGTTGATGAATG 13 0.325 No Hit GGAAACTACTGAAAATGAATCTGCTAATGAGGGTTACAAATTTGGTCAAG 13 0.325 No Hit CTATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAA 13 0.325 No Hit GGTCGCTTCTGCAACTGGATAACTAGCACTGAAAATCGTCTTTACATCGG 12 0.3 No Hit TATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAAT 12 0.3 No Hit GTTTTGATGATCCCTACCTTATTGACCGCAACTTCTGTATTTATTATCGC 12 0.3 No Hit GTTTCAACAACTCTCGTTCTTTACACTTCTTCTTGGCTGCTTGGCCTGTA 12 0.3 No Hit GGGAAACTACTGAAAATGAATCTGCTAATGAGGGTTACAAATTTGGTCAA 11 0.27499999999999997 No Hit GTTGATAGTCAAGGTCGCGTTATTAATACTTGGGCTGATATCATCAACCG 11 0.27499999999999997 No Hit GTTTCTGGTTCTTTACTTTATGGAAACAATATTATCTCTGGTGCTATTAT 11 0.27499999999999997 No Hit GCTAATTGTTCTACACTTCTTACTTGGTGTAGCTTGTTATATGGGTCGTG 10 0.25 No Hit TATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAA 10 0.25 No Hit AGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGATCT 10 0.25 No Hit GTTGCATATTCAGCTCCTGTTGCAGCTGCGACTGCTGTTTTCTTGATTTA 10 0.25 No Hit GTTAGGTGTAGCTGGTGTTTTCGGCGGTTCCCTATTCAGTGCTATGCATG 10 0.25 No Hit GTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGATCTTACCATGACTG 10 0.25 No Hit CAACATCCTTATGCATCCATTTCACATGTTAGGTGTAGCTGGTGTTTTCG 10 0.25 No Hit GGAAGAGACTTATAATATTGTGGCTGCTCATGGTTATTTTGGCCGATTAA 9 0.22499999999999998 No Hit CCTAAATGGATTCAATTTCAACCAATCTGTAGTTGATAGTCAAGGTCGCG 9 0.22499999999999998 No Hit TGGGAACTTAGTTTCCGTTTGGGTATGCGCCCTTGGATTGCTGTTGCATA 9 0.22499999999999998 No Hit TGAAAATGAATCTGCTAATGAGGGTTACAAATTTGGTCAAGAGGAAGAGA 9 0.22499999999999998 No Hit TTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAG 9 0.22499999999999998 No Hit GGTTATACAATGGTGGTCCTTATGAGCTAATTGTTCTACACTTCTTACTT 9 0.22499999999999998 No Hit ATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCTAAATGG 9 0.22499999999999998 No Hit GTACAAGCCTGTGGGGTCGCTTCTGCAACTGGATAACTAGCACTGAAAAT 9 0.22499999999999998 No Hit CTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGAT 8 0.2 No Hit AATGGATTCAATTTCAACCAATCTGTAGTTGATAGTCAAGGTCGCGTTAT 8 0.2 No Hit ATGCGCCCTTGGATTGCTGTTGCATATTCAGCTCCTGTTGCAGCTGCGAC 8 0.2 No Hit GGGAACTTAGTTTCCGTTTGGGTATGCGCCCTTGGATTGCTGTTGCATAT 8 0.2 No Hit TGTAGCTGGTGTTTTCGGCGGTTCCCTATTCAGTGCTATGCATGGTTCCT 8 0.2 No Hit GGTACTTTCAACTTTATGATTGTATTCCAGGCAGAGCACAACATCCTTAT 8 0.2 No Hit GTTCTTTACTTTATGGAAACAATATTATCTCTGGTGCTATTATTCCTACT 8 0.2 No Hit TTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAA 7 0.17500000000000002 No Hit TGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGATCTTACCATGACT 7 0.17500000000000002 No Hit CGTCTTTACATCGGATGGTTCGGTGTTTTGATGATCCCTACCTTATTGAC 7 0.17500000000000002 No Hit GGGTTACAAATTTGGTCAAGAGGAAGAGACTTATAATATTGTGGCTGCTC 7 0.17500000000000002 No Hit GGAATATCTGGTACTTTCAACTTTATGATTGTATTCCAGGCAGAGCACAA 7 0.17500000000000002 No Hit TATCTCTGGTGCTATTATTCCTACTTCTGCGGCAATCGGATTGCACTTTT 7 0.17500000000000002 No Hit ATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATA 7 0.17500000000000002 No Hit ATTATCTCTGGTGCTATTATTCCTACTTCTGCGGCAATCGGATTGCACTT 7 0.17500000000000002 No Hit GGATGGTTCGGTGTTTTGATGATCCCTACCTTATTGACCGCAACTTCTGT 7 0.17500000000000002 No Hit CTTAGTTTCCGTTTGGGTATGCGCCCTTGGATTGCTGTTGCATATTCAGC 7 0.17500000000000002 No Hit AGTCAAGGTCGCGTTATTAATACTTGGGCTGATATCATCAACCGTGCTAA 7 0.17500000000000002 No Hit GCCTTTAGGAATATCTGGTACTTTCAACTTTATGATTGTATTCCAGGCAG 7 0.17500000000000002 No Hit GTAGGAATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCT 7 0.17500000000000002 No Hit ATTCAGTGCTATGCATGGTTCCTTGGTAACCTCTAGTTTGATCAGGGAAA 7 0.17500000000000002 No Hit CTCTGGTGCTATTATTCCTACTTCTGCGGCAATCGGATTGCACTTTTACC 7 0.17500000000000002 No Hit GTTTGATCAGGGAAACTACTGAAAATGAATCTGCTAATGAGGGTTACAAA 7 0.17500000000000002 No Hit ATTCCTACTTCTGCGGCAATCGGATTGCACTTTTACCCAATTTGGGAAGC 6 0.15 No Hit GGTAACCTCTAGTTTGATCAGGGAAACTACTGAAAATGAATCTGCTAATG 6 0.15 No Hit CGGATTGCACTTTTACCCAATTTGGGAAGCTGCATCCGTTGATGAATGGT 6 0.15 No Hit CTGGTACTTTCAACTTTATGATTGTATTCCAGGCAGAGCACAACATCCTT 6 0.15 No Hit GCTTGGGAGTCCTTGCAATTTGAATAAACCAAGATCTTACCATGACTGCA 6 0.15 No Hit ATCGCCTTCATCGCAGCCCCTCCAGTAGATATTGATGGTATTCGCGAGCC 6 0.15 No Hit GAGACTTATAATATTGTGGCTGCTCATGGTTATTTTGGCCGATTAATCTT 6 0.15 No Hit GTGAGTGGGAACTTAGTTTCCGTTTGGGTATGCGCCCTTGGATTGCTGTT 6 0.15 No Hit GGCTGCTTGGCCTGTAGTAGGAATCTGGTTCACTGCTTTAGGTATTAGTA 6 0.15 No Hit GCTGTTTTCTTGATTTACCCTATTGGTCAAGGAAGCTTCTCTGATGGTAT 6 0.15 No Hit GTTCGATCCTGGCTCAGGATGAACGCTGGCGGCATGCTTAACACATGCAA 6 0.15 No Hit CTCCAGTAGATATTGATGGTATTCGCGAGCCTGTTTCTGGTTCTTTACTT 6 0.15 No Hit TTCCAATATGCTAGTTTCAACAACTCTCGTTCTTTACACTTCTTCTTGGC 6 0.15 No Hit CTAGCACTGAAAATCGTCTTTACATCGGATGGTTCGGTGTTTTGATGATC 6 0.15 No Hit GGAATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCTAAA 6 0.15 No Hit GTAGCTTGTTATATGGGTCGTGAGTGGGAACTTAGTTTCCGTTTGGGTAT 6 0.15 No Hit TGGGAGTCCTTGCAATTTGAATAAACCAAGATCTTACCATGACTGCAATT 6 0.15 No Hit CATCAACCGTGCTAATCTTGGTATGGAAGTAATGCACGAACGTAATGCTC 6 0.15 No Hit CAATGGTGGTCCTTATGAGCTAATTGTTCTACACTTCTTACTTGGTGTAG 6 0.15 No Hit GTTTCCGTTTGGGTATGCGCCCTTGGATTGCTGTTGCATATTCAGCTCCT 6 0.15 No Hit CGGATGGTTCGGTGTTTTGATGATCCCTACCTTATTGACCGCAACTTCTG 6 0.15 No Hit CGGCAATCGGATTGCACTTTTACCCAATTTGGGAAGCTGCATCCGTTGAT 6 0.15 No Hit TCTATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGA 6 0.15 No Hit CTGTAGTAGGAATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTC 6 0.15 No Hit CCGCAACTTCTGTATTTATTATCGCCTTCATCGCAGCCCCTCCAGTAGAT 5 0.125 No Hit GTCCTTATGAGCTAATTGTTCTACACTTCTTACTTGGTGTAGCTTGTTAT 5 0.125 No Hit ATTAATACTTGGGCTGATATCATCAACCGTGCTAATCTTGGTATGGAAGT 5 0.125 No Hit GTCGTGAGTGGGAACTTAGTTTCCGTTTGGGTATGCGCCCTTGGATTGCT 5 0.125 No Hit ATTTCAACCAATCTGTAGTTGATAGTCAAGGTCGCGTTATTAATACTTGG 5 0.125 No Hit GCAACTTCTGTATTTATTATCGCCTTCATCGCAGCCCCTCCAGTAGATAT 5 0.125 No Hit CTTCTGTATTTATTATCGCCTTCATCGCAGCCCCTCCAGTAGATATTGAT 5 0.125 No Hit CTTTAGGAATATCTGGTACTTTCAACTTTATGATTGTATTCCAGGCAGAG 5 0.125 No Hit GTTATATGGGTCGTGAGTGGGAACTTAGTTTCCGTTTGGGTATGCGCCCT 5 0.125 No Hit GGAAACAATATTATCTCTGGTGCTATTATTCCTACTTCTGCGGCAATCGG 5 0.125 No Hit CTCTGATGGTATGCCTTTAGGAATATCTGGTACTTTCAACTTTATGATTG 5 0.125 No Hit TGAATCTGCTAATGAGGGTTACAAATTTGGTCAAGAGGAAGAGACTTATA 5 0.125 No Hit CTTCTTACTTGGTGTAGCTTGTTATATGGGTCGTGAGTGGGAACTTAGTT 5 0.125 No Hit TACCATGACTGCAATTTTAGAGAGACGCGAAAGTACAAGCCTGTGGGGTC 5 0.125 No Hit GCTGCATCCGTTGATGAATGGTTATACAATGGTGGTCCTTATGAGCTAAT 5 0.125 No Hit GAAGAGACTTATAATATTGTGGCTGCTCATGGTTATTTTGGCCGATTAAT 5 0.125 No Hit GGCCGATTAATCTTCCAATATGCTAGTTTCAACAACTCTCGTTCTTTACA 5 0.125 No Hit GTTCTACACTTCTTACTTGGTGTAGCTTGTTATATGGGTCGTGAGTGGGA 5 0.125 No Hit ATCTATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTG 5 0.125 No Hit GGGTCGTGAGTGGGAACTTAGTTTCCGTTTGGGTATGCGCCCTTGGATTG 5 0.125 No Hit TTTACATCGGATGGTTCGGTGTTTTGATGATCCCTACCTTATTGACCGCA 5 0.125 No Hit TGATAGTCAAGGTCGCGTTATTAATACTTGGGCTGATATCATCAACCGTG 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.025 0.0 0.0 0.0 0.0 50-51 0.025 0.0 0.0 0.0 0.0 52-53 0.025 0.0 0.0 0.0 0.0 54-55 0.025 0.0 0.0 0.0 0.0 56-57 0.025 0.0 0.0 0.0 0.0 58-59 0.025 0.0 0.0 0.0 0.0 60-61 0.037500000000000006 0.0 0.0 0.0 0.0 62-63 0.05 0.0 0.0 0.0 0.0 64-65 0.05 0.0 0.0 0.0 0.0 66-67 0.05 0.0 0.0 0.0 0.0 68-69 0.1 0.0 0.0 0.0 0.0 70-71 0.125 0.0 0.0 0.0 0.0 72-73 0.175 0.0 0.0 0.0 0.0 74-75 0.2 0.0 0.0 0.0 0.0 76-77 0.21250000000000002 0.0 0.0 0.0 0.0 78-79 0.25 0.0 0.0 0.0 0.0 80-81 0.275 0.0 0.0 0.0 0.0 82-83 0.32499999999999996 0.0 0.0 0.0 0.0 84-85 0.4 0.0 0.0 0.0 0.0 86-87 0.5 0.0 0.0 0.0 0.0 88-89 0.6125 0.0 0.0 0.0 0.0 90-91 0.7625 0.0 0.0 0.0 0.0 92-93 0.95 0.0 0.0 0.0 0.0 94-95 1.1625 0.0 0.0 0.0 0.0 96-97 1.2999999999999998 0.0 0.0 0.0 0.0 98-99 1.5 0.0 0.0 0.0 0.0 100-101 1.7375 0.0 0.0 0.0 0.0 102-103 1.875 0.0 0.0 0.0 0.0 104-105 2.1125 0.0 0.0 0.0 0.0 106-107 2.4375 0.0 0.0 0.0 0.0 108-109 2.7 0.0 0.0 0.0 0.0 110-111 3.05 0.0 0.0 0.0 0.0 112-113 3.3625 0.0 0.0 0.0 0.0 114-115 3.825 0.0 0.0 0.0 0.0 116-117 4.2125 0.0 0.0 0.0 0.0 118-119 4.75 0.0 0.0 0.0 0.0 120-121 5.2875 0.0 0.0 0.0 0.0 122-123 5.9 0.0 0.0 0.0 0.0 124-125 6.55 0.0 0.0 0.0 0.0 126-127 7.5625 0.0 0.0 0.0 0.0 128-129 8.3 0.0 0.0 0.0 0.0 130-131 8.8875 0.0 0.0 0.0 0.0 132-133 9.7625 0.0 0.0 0.0 0.0 134-135 10.3125 0.0 0.0 0.0 0.0 136-137 11.0 0.0 0.0 0.0 0.0 138-139 11.525 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content pass >>END_MODULE Read 1034826 spots for SRR6941584.sra Written 1034826 spots for SRR6941584.sra Read 1034826 spots for SRR6941584.sra Written 1034826 spots for SRR6941584.sra Read 1034826 spots for SRR6941584.sra Written 1034826 spots for SRR6941584.sra Read 1034826 spots for SRR6941584.sra Written 1034826 spots for SRR6941584.sra Read 1034826 spots for SRR6941584.sra Written 1034826 spots for SRR6941584.sra Read 1034826 spots for SRR6941584.sra Written 1034826 spots for SRR6941584.sra Read 1034826 spots for SRR6941584.sra Written 1034826 spots for SRR6941584.sra Read 1034826 spots for SRR6941584.sra Written 1034826 spots for SRR6941584.sra Read 1034826 spots for SRR6941584.sra Written 1034826 spots for SRR6941584.sra Read 1034826 spots for SRR6941584.sra Written 1034826 spots for SRR6941584.sra Read 1034835 spots for SRR6941584.sra Written 1034835 spots for SRR6941584.sra Read 1034826 spots for SRR6941584.sra Written 1034826 spots for SRR6941584.sra Read 1034826 spots for SRR6941584.sra Written 1034826 spots for SRR6941584.sra Read 1034826 spots for SRR6941584.sra Written 1034826 spots for SRR6941584.sra Read 1034826 spots for SRR6941584.sra Written 1034826 spots for SRR6941584.sra Read 1034826 spots for SRR6941584.sra Written 1034826 spots for SRR6941584.sra Read 1034826 spots for SRR6941584.sra Written 1034826 spots for SRR6941584.sra Read 1034826 spots for SRR6941584.sra Written 1034826 spots for SRR6941584.sra Read 1034826 spots for SRR6941584.sra Written 1034826 spots for SRR6941584.sra Read 1034826 spots for SRR6941584.sra Written 1034826 spots for SRR6941584.sra SRR ids: ['SRR6941584.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_prsas219 SRR6941584.sra spots: 20696529 blocks: [[1, 1034826], [1034827, 2069652], [2069653, 3104478], [3104479, 4139304], [4139305, 5174130], [5174131, 6208956], [6208957, 7243782], [7243783, 8278608], [8278609, 9313434], [9313435, 10348260], [10348261, 11383086], [11383087, 12417912], [12417913, 13452738], [13452739, 14487564], [14487565, 15522390], [15522391, 16557216], [16557217, 17592042], [17592043, 18626868], [18626869, 19661694], [19661695, 20696529]] SRR6941584 file size 6991674 SRR6941584 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR6941584 SRR6941584_1.fastq SRR6941584_2.fastq Input file: SRR6941584_1.fastq Paired file: SRR6941584_2.fastq trimmed: SRR6941584-trimmed-pair1.fastq, SRR6941584-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Fri Dec 6 12:13:26 2024 >> started Fri Dec 6 12:13:49 2024 >> done (23.313s) 20696529 read pairs processed; of these: 16453 ( 0.08%) short read pairs filtered out after trimming by size control 13524 ( 0.07%) empty read pairs filtered out after trimming by size control 20666552 (99.86%) read pairs available; of these: 10571505 (51.15%) trimmed read pairs available after processing 10095047 (48.85%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 1 0.00% 19 1 0.00% 20 2 0.00% 21 1 0.00% 22 1 0.00% 23 3 0.00% 24 2 0.00% 25 3 0.00% 26 3 0.00% 27 31 0.00% 28 6 0.00% 29 5 0.00% 30 1 0.00% 31 11 0.00% 32 7 0.00% 33 5 0.00% 34 9 0.00% 35 9 0.00% 36 9 0.00% 37 12 0.00% 38 8 0.00% 39 10 0.00% 40 23 0.00% 41 14 0.00% 42 24 0.00% 43 23 0.00% 44 26 0.00% 45 41 0.00% 46 30 0.00% 47 41 0.00% 48 50 0.00% 49 51 0.00% 50 78 0.00% 51 62 0.00% 52 99 0.00% 53 109 0.00% 54 118 0.00% 55 182 0.00% 56 142 0.00% 57 184 0.00% 58 180 0.00% 59 284 0.00% 60 324 0.00% 61 338 0.00% 62 469 0.00% 63 542 0.00% 64 571 0.00% 65 727 0.00% 66 811 0.00% 67 841 0.00% 68 979 0.00% 69 1092 0.01% 70 1339 0.01% 71 1523 0.01% 72 1974 0.01% 73 2090 0.01% 74 2383 0.01% 75 2677 0.01% 76 3017 0.01% 77 3437 0.02% 78 3792 0.02% 79 4223 0.02% 80 4855 0.02% 81 5570 0.03% 82 6334 0.03% 83 6656 0.03% 84 8158 0.04% 85 9998 0.05% 86 10351 0.05% 87 11328 0.05% 88 12936 0.06% 89 13429 0.06% 90 15525 0.08% 91 15682 0.08% 92 18578 0.09% 93 19091 0.09% 94 20596 0.10% 95 23312 0.11% 96 22400 0.11% 97 23100 0.11% 98 25041 0.12% 99 26337 0.13% 100 27539 0.13% 101 29335 0.14% 102 32087 0.16% 103 31681 0.15% 104 34977 0.17% 105 37479 0.18% 106 37546 0.18% 107 39014 0.19% 108 41031 0.20% 109 42939 0.21% 110 43548 0.21% 111 46041 0.22% 112 46970 0.23% 113 45959 0.22% 114 53251 0.26% 115 56111 0.27% 116 58697 0.28% 117 58580 0.28% 118 56927 0.28% 119 58078 0.28% 120 58908 0.29% 121 61318 0.30% 122 72110 0.35% 123 70067 0.34% 124 72779 0.35% 125 77548 0.38% 126 72217 0.35% 127 76065 0.37% 128 77144 0.37% 129 83112 0.40% 130 78086 0.38% 131 85093 0.41% 132 89470 0.43% 133 83111 0.40% 134 93792 0.45% 135 90678 0.44% 136 94760 0.46% 137 94996 0.46% 138 106062 0.51% 139 109875 0.53% 140 111621 0.54% 141 130399 0.63% 142 131867 0.64% 143 147861 0.72% 144 162566 0.79% 145 197998 0.96% 146 230544 1.12% 147 290898 1.41% 148 441879 2.14% 149 830402 4.02% 150 5036162 24.37% 151 10095047 48.85% 20666552 reads passed initial QC criterion=sequence-density sequence-density=0.46 sequence-density-rank=1 fanout-score=1.97 fanout-score-rank=26 prefix-density=0.46 prefix-fanout=2.0 sequence=GTATTTAGCCTTG criterion=fanout-score sequence-density=0.01 sequence-density-rank=37 fanout-score=44.70 fanout-score-rank=1 prefix-density=0.06 prefix-fanout=6.2 sequence=AAAAAAAAAGGGGGGTAAGGACCCGCTAAGCTCCTACTTTTTCATGTTTCCAATCCGATCCCTCCGATTACTATAGAGATGAACCCAATCCAGAATATGAACCATAAAAGAAAACACCTACTAAACCAATCACAAGAATACCAGTTACCGTACCTATCAGCCAAAGAGGAATTCTTCCAGTAGTATCGGCCATTTCCCCTACTTTCCTCCACATTTTATCAAGTGGTCATGCTAGAGACAAAAACAGTCATGGATAGTTATGTTATAAGGATGGTATCCTTCCAAATGGGATAAGAGAGTTCTTACTACTCTCTTCTTTTCTCTCAATTAAAGAAGTAATTGGAAAACAAAACAGCAAGTACAAAAATGAGTAATAAACCCCAGTATAGACTGGTACGATTCAATTCAACATTTTGTTCATTCGGGTTTGATTGTGTCATAGTTCTATAGTTGGAATTTAGTTTATCGTTGGATGAACTGCATTGCTGATATTGATCCCAAGAAAAAAACA criterion=sequence-density sequence-density=0.40 sequence-density-rank=1 fanout-score=2.68 fanout-score-rank=20 prefix-density=1.06 prefix-fanout=1.0 sequence=TTGCGTAGTGGATCTGCTGGGGCCTATGCGAAAGCTGGGCCTCACGGATCCTAGAGCGGCAGGCACCGCGTGAGGCTG criterion=fanout-score sequence-density=0.02 sequence-density-rank=31 fanout-score=24.04 fanout-score-rank=1 prefix-density=0.53 prefix-fanout=1.1 sequence=TGGTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGTTAACGAACGAGACCTCAGCCTGCTAACTAGCTATGCGGAGCCATCCCTCCGCAGCTAGCTTCTTAGAGGGACTATCGCCGTTTAGGCGACGGAAGTTTGAGGCAATAACAGGTCTGTGATGCCCTTAGATGTTCTGGGCCGCACGCGCGCTACACTGATGTATTCAACGAGTATATAGCCTTGGCCGACAGGCCCGGGTAATCTTGGGAAATTTCATCGTGATGGGGATAGATCATTGCAATTGTTGGTCTTCAACGAGGAATGCCTAGTAAGCGCGAGTCATCAGCTCGCGTTGACTACGTCCCTGCCCTTTGTACACACC SRR6941584 testing PE reads STAR mapping to Ensembl genome Started job on | Dec 06 12:14:22 Started mapping on | Dec 06 12:14:23 Finished on | Dec 06 12:16:25 Mapping speed, Million of reads per hour | 609.83 Number of input reads | 20666552 Average input read length | 291 UNIQUE READS: Uniquely mapped reads number | 12796350 Uniquely mapped reads % | 61.92% Average mapped length | 293.58 Number of splices: Total | 2464402 Number of splices: Annotated (sjdb) | 2224875 Number of splices: GT/AG | 2344020 Number of splices: GC/AG | 28382 Number of splices: AT/AC | 9017 Number of splices: Non-canonical | 82983 Mismatch rate per base, % | 0.30% Deletion rate per base | 0.02% Deletion average length | 1.98 Insertion rate per base | 0.02% Insertion average length | 2.44 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 6639500 % of reads mapped to multiple loci | 32.13% Number of reads mapped to too many loci | 55581 % of reads mapped to too many loci | 0.27% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 3.99% % of reads unmapped: other | 1.69% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 1241085 1241085 1241085 N_multimapping 6639500 6639500 6639500 N_noFeature 5270176 12344904 5459617 N_ambiguous 471509 7441 215090 UnstrandedReadsAssigned:7054665 PositiveStrandReadsAssigned:444005 NegativeStrandReadsAssigned:7121643 Dataset is classified negative stranded MeadianReadLen=151 20thPercentileLength=149 echo kmer=145 SRR6941584 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,972 [index] number of k-mers: 66,720,672 [index] number of equivalence classes: 111,837 [quant] running in paired-end mode [quant] will process pair 1: SRR6941584-trimmed-pair1.fastq SRR6941584-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 20,666,552 reads, 11,297,109 reads pseudoaligned [quant] estimated average fragment length: 228.022 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,035 rounds 52973 SRR6941584.ke.tsv 35125 SRR6941584.se.tsv 88098 total ==> SRR6941584.ke.tsv <== target_id length eff_length est_counts tpm PNS24245 936 709.682 0 0 PNS24247 1044 816.978 8.61206 0.916476 PNS24249 1928 1700.98 18.9305 0.96758 PNS24246 1044 816.978 8.61206 0.916476 PNS24248 1044 816.978 8.61206 0.916476 PNS24244 1471 1243.98 7.23335 0.505535 PNS24243 293 109.458 0 0 KQK14069 1603 1375.98 789.478 49.8831 KQK14071 474 259.757 12.8998 4.31758 ==> SRR6941584.se.tsv <== BRADI_1g14170v3 986 BRADI_1g53295v3 27 BRADI_1g59795v3 13 BRADI_1g07683v3 0 BRADI_1g00485v3 0 BRADI_1g20270v3 45 BRADI_1g74790v3 14 BRADI_1g09890v3 0 BRADI_1g77505v3 43 BRADI_1g48960v3 0 SRR6941584 completed mapping pipeline successfully