Starting /dee2/code/volunteer_pipeline.sh SRR6941585 current disk space = 1551480795136 free memory = 1602304296 SRR6941585 SRAfilesize ff03cc73856b1360b9df85cd63bb28e7 SRR6941585.sra SRR6941585.sra file validated SRR6941585 is paired end SRR6941585 is conventional basespace SRR6941585 read1 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR6941585_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 51 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.6175 34.0 33.0 34.0 32.0 34.0 2 32.997 34.0 33.0 34.0 32.0 34.0 3 33.17225 34.0 33.0 34.0 32.0 34.0 4 33.35575 34.0 33.0 34.0 33.0 34.0 5 33.39825 34.0 33.0 34.0 33.0 34.0 6 37.22775 38.0 38.0 38.0 36.0 38.0 7 37.51825 38.0 38.0 38.0 37.0 38.0 8 37.5875 38.0 38.0 38.0 38.0 38.0 9 37.6505 38.0 38.0 38.0 38.0 38.0 10-14 37.58605 38.0 38.0 38.0 38.0 38.0 15-19 37.5892 38.0 38.0 38.0 38.0 38.0 20-24 37.56385000000001 38.0 38.0 38.0 38.0 38.0 25-29 37.55165 38.0 38.0 38.0 38.0 38.0 30-34 37.4152 38.0 38.0 38.0 37.8 38.0 35-39 37.53189999999999 38.0 38.0 38.0 38.0 38.0 40-44 37.491749999999996 38.0 38.0 38.0 38.0 38.0 45-49 37.442699999999995 38.0 38.0 38.0 37.6 38.0 50-54 37.513200000000005 38.0 38.0 38.0 38.0 38.0 55-59 37.4431 38.0 38.0 38.0 37.8 38.0 60-64 37.4338 38.0 38.0 38.0 37.6 38.0 65-69 37.4019 38.0 38.0 38.0 37.6 38.0 70-74 37.33164999999999 38.0 38.0 38.0 37.2 38.0 75-79 37.313050000000004 38.0 38.0 38.0 37.0 38.0 80-84 37.3225 38.0 38.0 38.0 37.0 38.0 85-89 37.25085 38.0 38.0 38.0 37.0 38.0 90-94 37.16795 38.0 38.0 38.0 36.2 38.0 95-99 37.19975 38.0 38.0 38.0 36.2 38.0 100-104 37.09929999999999 38.0 38.0 38.0 36.0 38.0 105-109 36.99745 38.0 38.0 38.0 35.6 38.0 110-114 36.7025 38.0 38.0 38.0 35.0 38.0 115-119 36.640499999999996 38.0 38.0 38.0 34.8 38.0 120-124 36.7789 38.0 38.0 38.0 35.0 38.0 125-129 36.676649999999995 38.0 38.0 38.0 35.0 38.0 130-134 36.5769 38.0 38.0 38.0 34.2 38.0 135-139 36.447199999999995 38.0 38.0 38.0 34.0 38.0 140-144 36.318450000000006 38.0 38.0 38.0 33.8 38.0 145-149 36.0118 38.0 38.0 38.0 33.2 38.0 150-151 32.416125 35.5 33.5 38.0 17.0 38.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 12 1.0 13 0.0 14 1.0 15 1.0 16 0.0 17 2.0 18 3.0 19 0.0 20 1.0 21 0.0 22 2.0 23 3.0 24 4.0 25 6.0 26 9.0 27 9.0 28 13.0 29 19.0 30 29.0 31 33.0 32 51.0 33 55.0 34 104.0 35 145.0 36 374.0 37 3135.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 48.83966244725738 9.941983122362869 5.116033755274262 36.10232067510549 2 23.075000000000003 11.375 34.175 31.374999999999996 3 21.625 16.900000000000002 25.35 36.125 4 26.375 23.474999999999998 21.975 28.175 5 25.575 29.45 22.7 22.275 6 20.825 30.3 24.224999999999998 24.65 7 16.5 20.275000000000002 41.025 22.2 8 19.8 18.575 32.2 29.425 9 19.175 17.825 33.900000000000006 29.099999999999998 10-14 23.849999999999998 24.62 23.544999999999998 27.985 15-19 23.275000000000002 23.330000000000002 26.369999999999997 27.025 20-24 22.915 23.91 25.41 27.765 25-29 23.755000000000003 22.585 25.979999999999997 27.68 30-34 23.835 23.77 25.435000000000002 26.96 35-39 23.09 23.23 25.669999999999998 28.01 40-44 23.46 23.16 25.91 27.47 45-49 21.73717371737174 23.15231523152315 27.527752775277527 27.582758275827583 50-54 23.044999999999998 22.18 25.990000000000002 28.785 55-59 23.22 22.814999999999998 26.16 27.805000000000003 60-64 22.795 22.355 26.565 28.285 65-69 22.79 23.28 25.074999999999996 28.854999999999997 70-74 24.54 23.26 23.880000000000003 28.32 75-79 22.85 24.46 24.89 27.800000000000004 80-84 23.54 24.08 24.775 27.605 85-89 23.255 23.315 25.55 27.88 90-94 23.1 24.55 24.279999999999998 28.07 95-99 23.22 24.41 25.105 27.265 100-104 23.39584896224056 23.645911477869465 24.186046511627907 28.772193048262068 105-109 23.810000000000002 23.315 25.355 27.52 110-114 23.59437751004016 24.076305220883533 25.18574297188755 27.143574297188756 115-119 23.407882860294855 24.039715174004613 24.842041921572562 27.710360044127967 120-124 23.57 24.51 23.294999999999998 28.625 125-129 23.544999999999998 24.275 23.169999999999998 29.01 130-134 24.62 23.425 23.23 28.725 135-139 23.775 24.025 24.48 27.72 140-144 24.22 23.919999999999998 24.08 27.779999999999998 145-149 23.49 24.0 24.245 28.265 150-151 22.3 25.4 23.425 28.875 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.5 21 1.5 22 1.5 23 1.0 24 1.5 25 2.0 26 2.0 27 4.0 28 7.5 29 8.0 30 7.0 31 5.5 32 7.5 33 9.0 34 12.0 35 20.0 36 42.0 37 80.5 38 82.5 39 78.5 40 95.0 41 104.0 42 90.0 43 80.0 44 92.0 45 101.5 46 101.5 47 108.0 48 117.5 49 114.0 50 123.5 51 148.0 52 178.5 53 195.0 54 222.5 55 285.0 56 299.5 57 249.0 58 225.0 59 192.5 60 133.5 61 89.5 62 50.0 63 33.5 64 27.0 65 18.5 66 19.5 67 17.0 68 14.0 69 14.0 70 13.0 71 18.0 72 14.0 73 5.0 74 9.0 75 12.0 76 7.5 77 3.5 78 3.0 79 1.5 80 0.5 81 0.5 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content warn #Base N-Count 1 5.2 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.01 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.025 105-109 0.0 110-114 0.4 115-119 0.29 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 65.975 #Duplication Level Percentage of deduplicated Percentage of total 1 76.69571807502842 50.6 2 12.845774914740431 16.950000000000003 3 4.736642667677151 9.375 4 2.57673361121637 6.800000000000001 5 0.9094353921940129 3.0 6 0.6062902614626753 2.4 7 0.5305039787798408 2.45 8 0.30314513073133764 1.6 9 0.15157256536566882 0.8999999999999999 >10 0.6441834028040925 5.925 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCATTAAT 26 0.65 No Hit GTCGGTTTCGGGTACAGGTACCCTTTTGTTGAAGGTCGTTCGAGCTTTTC 26 0.65 No Hit GCGGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTACG 21 0.525 No Hit GTACAAGGCCCGGGAACGGATTCACCGCCGTATGGCTGACCGGCGATTAC 20 0.5 No Hit GGCATGATGACTTGGCCTCATCCTCTCCTTCCTCCGGCTTAACACCGGCG 13 0.325 No Hit GTACAAAGGGCAGGGACGTAGTCAACGCGAGCTGATGACTCGCGCTTACT 13 0.325 No Hit GTGCGACGTGGGGCTGGATCTCAGTGGATCGTGGCAGCAAGGCCACTCTG 13 0.325 No Hit CTTTCTTTTCCTCTGGCTACTAAGATGTTTCAGTTCGCCAGGTTGTCTCT 12 0.3 No Hit GCTTTCTTTTCCTCTGGCTACTAAGATGTTTCAGTTCGCCAGGTTGTCTC 11 0.27499999999999997 No Hit GCCAAAATAACCATGAGCAGCCACAATATTATAAGTCTCTTCCTCTTGAC 11 0.27499999999999997 No Hit GTTTACGGCTAGGACTACTGGGGTCTCTAATCCCATTTGCTCCCCTAGCT 11 0.27499999999999997 No Hit CTCCACCGCTTGTGCGGGCCCCCGTCAATTCCTTTGAGTTTCATTCTTGC 10 0.25 No Hit ACATGCTCCACCGCTTGTGCGGGCCCCCGTCAATTCCTTTGAGTTTCATT 10 0.25 No Hit GGCTGATCATCCTCTCGGACCAGCTACTGATCATCGCCTTGGTAAGCTAT 10 0.25 No Hit GGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTACGTT 10 0.25 No Hit GTTGAAACTAGCATATTGGAAGATTAATCGGCCAAAATAACCATGAGCAG 10 0.25 No Hit CCCCACTGCTGCCTCCCGTAGGAGTCTGGGCCGTGTCTCAGTCCCAGTGT 10 0.25 No Hit GTGCTCTGCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTCCTA 9 0.22499999999999998 No Hit CTAGCTTTCGTCTCTCAGTGTCAGTGTCGGCCCAGCAGAGTGCTTTCGCC 9 0.22499999999999998 No Hit GCCACGCTTTCACGGTTCGTATTCGTACTGGAAATCAGAATCAAACGAGC 9 0.22499999999999998 No Hit CCGGGAACGGATTCACCGCCGTATGGCTGACCGGCGATTACTAGCGATTC 9 0.22499999999999998 No Hit GCTCCACCGCTTGTGCGGGCCCCCGTCAATTCCTTTGAGTTTCATTCTTG 8 0.2 No Hit GCCAGCTCCTATAGTGTGACGGGCGGTGTGTACAAGGCCCGGGAACGGAT 8 0.2 No Hit GCAAAGGATTCAGCCCGCCGCCCGTGGGGAAGGGAGCTTCGAGGCGGCCG 8 0.2 No Hit GTTTCTTCTCCGAGAAAAGAAGTTGACGACCCGTAGGCCTTCCACCTCCA 8 0.2 No Hit GCTTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCA 8 0.2 No Hit GGCAATTTCAAGCACTCTTTGACTCTCTTTTCAAAGTCCTTTTCATCTTT 8 0.2 No Hit GTCGTCTGCAAAGGATTCAGCCCGCCGCCCGTGGGGAAGGGAGCTTCGAG 8 0.2 No Hit CCTGTGTCGGTTTCGGGTACAGGTACCCTTTTGTTGAAGGTCGTTCGAGC 8 0.2 No Hit CACCGCTTGTGCGGGCCCCCGTCAATTCCTTTGAGTTTCATTCTTGCGAA 7 0.17500000000000002 No Hit GCACTCTTTGACTCTCTTTTCAAAGTCCTTTTCATCTTTCCCTCGCGGTA 7 0.17500000000000002 No Hit CCTAGATGTCCAGTCAACTGCTGCGCCTCAACGCATTTCGGGGAGAACCA 7 0.17500000000000002 No Hit GCGGTCAATAAGGTAGGGATCATCAAAACACCGAACCATCCGATGTAAAG 7 0.17500000000000002 No Hit GTCAATTCCTTTGAGTTTCATTCTTGCGAACGTACTCCCCAGGCGGGATA 7 0.17500000000000002 No Hit GTTCGAGCTTTTCCTGGGAGTATGGCATCGGTTACATACTTCAGTGCCGT 7 0.17500000000000002 No Hit CCACATGCTCCACCGCTTGTGCGGGCCCCCGTCAATTCCTTTGAGTTTCA 7 0.17500000000000002 No Hit GCCGTGTCTCAGTCCCAGTGTGGCTGATCATCCTCTCGGACCAGCTACTG 7 0.17500000000000002 No Hit GGGCTTACTACTTATATGCTTTCAGCAGTTATCCTCTCCGCACTTGGCTA 7 0.17500000000000002 No Hit ATCACGATGAAATTTCCCAAGATTACCCGGGCCTGTCGGCCAAGGCTATA 7 0.17500000000000002 No Hit GTCCAGTCAACTGCTGCGCCTCAACGCATTTCGGGGAGAACCAGCTAGCT 7 0.17500000000000002 No Hit GCCGATGCTTATTCCTCAGATACCGTCATTGTTTCTTCTCCGAGAAAAGA 7 0.17500000000000002 No Hit GCTCTGCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTCCTAAA 7 0.17500000000000002 No Hit GTGGGCTTACTACTTATATGCTTTCAGCAGTTATCCTCTCCGCACTTGGC 7 0.17500000000000002 No Hit CGGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTACGT 6 0.15 No Hit GTGTACAAGGCCCGGGAACGGATTCACCGCCGTATGGCTGACCGGCGATT 6 0.15 No Hit CTTCAAACATGGCCAGCTCCTATAGTGTGACGGGCGGTGTGTACAAGGCC 6 0.15 No Hit GTCAATTCCTTTAAGTTTCAGCCTTGCGACCATACTCCCCCCGGAACCCA 6 0.15 No Hit GGGCGGTGTGTACAAAGGGCAGGGACGTAGTCAACGCGAGCTGATGACTC 6 0.15 No Hit GCCACCTACAGACGCTTTACGCCCAATCATTCCGGATAACGCTTGCATCC 6 0.15 No Hit GTTACGACTTCTCCTTCCTCTAAATGATAAGGTTCAATGGACTTCTCGCG 6 0.15 No Hit GTGTGTACAAGGCCCGGGAACGGATTCACCGCCGTATGGCTGACCGGCGA 6 0.15 No Hit GCCCCATGCTACTCGGGTCAGAGCGTAAGCTAGTGATGCTTTCGGCTACT 6 0.15 No Hit CTTTCCCTCACGGTACTACTTCGCTATCGGTCACCCAGGAGTATTTAGCC 6 0.15 No Hit CTCAGATACCGTCATTGTTTCTTCTCCGAGAAAAGAAGTTGACGACCCGT 6 0.15 No Hit GTCGGTTCGGACCTCTGCTTAGTTTCATCCAAGCTTCATCCTGGTCATGG 6 0.15 No Hit GGCTAACCTAGCCTCCTCCGTCCCTCCGTACCAACAAGGGGTAGTACAGG 6 0.15 No Hit CTTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCAT 6 0.15 No Hit CCCTAGAGTAACTTTTATCCGTTGAGCGACGGCCCTTCCACTCGGCACCG 6 0.15 No Hit ACCACATGCTCCACCGCTTGTGCGGGCCCCCGTCAATTCCTTTGAGTTTC 6 0.15 No Hit GTTAGCTACAGCACTGCACGGGTCGAGTCGCACAGCACCTAGTATCCATC 5 0.125 No Hit GGGCGGTGTGTACAAGGCCCGGGAACGGATTCACCGCCGTATGGCTGACC 5 0.125 No Hit TGGGAATCTCCGGATCTATGCTTATTTTCAACTCCCCGAAGCATTTCGTC 5 0.125 No Hit CTGCGATGAAGGCGATAATAAATACAGAAGTTGCGGTCAATAAGGTAGGG 5 0.125 No Hit CGTCAATTCCTTTGAGTTTCATTCTTGCGAACGTACTCCCCAGGCGGGAT 5 0.125 No Hit ACCTAAGCTGCGCAGGAAAGGCCCAAAGCCAATCCCAGGGAACAGTAAAG 5 0.125 No Hit CACCGCTCCACCGGAAATTCCCTCTGCCCCTACCGTACTCCAGCTTGGTA 5 0.125 No Hit GGCGCATACCCAAACGGAAACTAAGTTCCCACTCACGACCCATATAACAA 5 0.125 No Hit CCCGACTGTCCCTATTAATCATTACTCCGATCCCGAAGGCCAACACAATA 5 0.125 No Hit CCCACCTGTGTCGGTTTCGGGTACAGGTACCCTTTTGTTGAAGGTCGTTC 5 0.125 No Hit GGCATACCATCAGAGAAGCTTCCTTGACCAATAGGGTAAATCAAGAAAAC 5 0.125 No Hit GCCGACCTTGACCCCTGTTATTTTGGGGTCATATCTAGTATTCAGAGTTT 5 0.125 No Hit GCTAGTTATCCAGTTGCAGAAGCGACCCCACAGGCTTGTACTTTCGCGTC 5 0.125 No Hit CATGCTCCACCGCTTGTGCGGGCCCCCGTCAATTCCTTTGAGTTTCATTC 5 0.125 No Hit CATATAACAAGCTACACCAAGTAAGAAGTGTAGAACAATTAGCTCATAAG 5 0.125 No Hit CCCTACCGTACTCCAGCTTGGTAGTTTCCACCGCCTGTCCAGGGTTGAGC 5 0.125 No Hit CCCACTGCTGCCTCCCGTAGGAGTCTGGGCCGTGTCTCAGTCCCAGTGTG 5 0.125 No Hit GTGCTAGTTATCCAGTTGCAGAAGCGACCCCACAGGCTTGTACTTTCGCG 5 0.125 No Hit GGGTGGGCTTACTACTTATATGCTTTCAGCAGTTATCCTCTCCGCACTTG 5 0.125 No Hit CTCCACGCGGCATTGCTCCGTCAGGCTTTCGCCCATTGCGGAAAATTCCC 5 0.125 No Hit GCACGTGTGTCGCCCAGGGCATAAGGGGCATGATGACTTGGCCTCATCCT 5 0.125 No Hit GTGAGCTATTACGCACTCTTTAAAGGGTGGCTGCTTCTAGGCAAACCTCC 5 0.125 No Hit GCCTCATCCTCTCCTTCCTCCGGCTTAACACCGGCGGTCTGTTCAGGGTT 5 0.125 No Hit CTCGCGAATACCATCAATATCTACTGGAGGGGCTGCGATGAAGGCGATAA 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0125 0.0 0.0 0.0 0.0 68-69 0.025 0.0 0.0 0.0 0.0 70-71 0.025 0.0 0.0 0.0 0.0 72-73 0.075 0.0 0.0 0.0 0.0 74-75 0.1 0.0 0.0 0.0 0.0 76-77 0.175 0.0 0.0 0.0 0.0 78-79 0.175 0.0 0.0 0.0 0.0 80-81 0.2625 0.0 0.0 0.0 0.0 82-83 0.3375 0.0 0.0 0.0 0.0 84-85 0.3875 0.0 0.0 0.0 0.0 86-87 0.5125 0.0 0.0 0.0 0.0 88-89 0.6499999999999999 0.0 0.0 0.0 0.0 90-91 0.9125 0.0 0.0 0.0 0.0 92-93 1.125 0.0 0.0 0.0 0.0 94-95 1.4125 0.0 0.0 0.0 0.0 96-97 1.625 0.0 0.0 0.0 0.0 98-99 2.0 0.0 0.0 0.0 0.0 100-101 2.3499999999999996 0.0 0.0 0.0 0.0 102-103 2.7 0.0 0.0 0.0 0.0 104-105 3.1625 0.0 0.0 0.0 0.0 106-107 3.5125 0.0 0.0 0.0 0.0 108-109 3.9625 0.0 0.0 0.0 0.0 110-111 4.387499999999999 0.0 0.0 0.0 0.0 112-113 5.025 0.0 0.0 0.0 0.0 114-115 5.5 0.0 0.0 0.0 0.0 116-117 5.975 0.0 0.0 0.0 0.0 118-119 6.675 0.0 0.0 0.0 0.0 120-121 7.15 0.0 0.0 0.0 0.0 122-123 7.8875 0.0 0.0 0.0 0.0 124-125 8.475 0.0 0.0 0.0 0.0 126-127 9.125 0.0 0.0 0.0 0.0 128-129 9.774999999999999 0.0 0.0 0.0 0.0 130-131 10.587499999999999 0.0 0.0 0.0 0.0 132-133 11.425 0.0 0.0 0.0 0.0 134-135 11.975 0.0 0.0 0.0 0.0 136-137 12.8875 0.0 0.0 0.0 0.0 138-139 13.6125 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTGCGAC 10 0.0054187924 156.51352 1 TGCGACG 10 0.006862618 144.77501 2 TGGGGCT 10 0.006862618 144.77501 9 GTGGGGC 10 0.006862618 144.77501 8 GACGTGG 10 0.006862618 144.77501 5 TCTCGTA 40 0.0056559923 54.290627 145 AAAAAAA 30 0.0014569166 24.12917 140-144 >>END_MODULE SRR6941585 read2 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR6941585_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 51 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 33.1605 34.0 33.0 34.0 33.0 34.0 2 33.255 34.0 33.0 34.0 33.0 34.0 3 33.275 34.0 33.0 34.0 33.0 34.0 4 33.17475 34.0 33.0 34.0 33.0 34.0 5 33.226 34.0 33.0 34.0 33.0 34.0 6 37.381 38.0 38.0 38.0 38.0 38.0 7 37.328 38.0 38.0 38.0 38.0 38.0 8 37.30475 38.0 38.0 38.0 38.0 38.0 9 37.3095 38.0 38.0 38.0 38.0 38.0 10-14 37.307399999999994 38.0 38.0 38.0 38.0 38.0 15-19 37.28245 38.0 38.0 38.0 37.8 38.0 20-24 37.2866 38.0 38.0 38.0 37.8 38.0 25-29 37.26530000000001 38.0 38.0 38.0 38.0 38.0 30-34 37.291149999999995 38.0 38.0 38.0 37.8 38.0 35-39 37.23075 38.0 38.0 38.0 38.0 38.0 40-44 37.24155 38.0 38.0 38.0 37.6 38.0 45-49 37.2693 38.0 38.0 38.0 37.8 38.0 50-54 37.238800000000005 38.0 38.0 38.0 37.6 38.0 55-59 37.15415 38.0 38.0 38.0 37.0 38.0 60-64 37.08175 38.0 38.0 38.0 37.0 38.0 65-69 37.074850000000005 38.0 38.0 38.0 37.0 38.0 70-74 37.056000000000004 38.0 38.0 38.0 37.0 38.0 75-79 37.0175 38.0 38.0 38.0 36.8 38.0 80-84 37.027699999999996 38.0 38.0 38.0 36.8 38.0 85-89 36.96495 38.0 38.0 38.0 36.0 38.0 90-94 36.96185 38.0 38.0 38.0 36.0 38.0 95-99 36.82769999999999 38.0 38.0 38.0 35.4 38.0 100-104 36.6988 38.0 38.0 38.0 35.0 38.0 105-109 36.52935 38.0 38.0 38.0 34.8 38.0 110-114 36.20784999999999 38.0 38.0 38.0 34.0 38.0 115-119 36.07189999999999 38.0 38.0 38.0 33.8 38.0 120-124 36.0818 38.0 38.0 38.0 33.6 38.0 125-129 36.1451 38.0 38.0 38.0 34.0 38.0 130-134 36.0077 38.0 38.0 38.0 33.2 38.0 135-139 35.8122 38.0 38.0 38.0 32.6 38.0 140-144 35.516400000000004 38.0 37.2 38.0 31.2 38.0 145-149 34.7168 38.0 36.0 38.0 28.4 38.0 150-151 29.89825 35.5 27.0 38.0 14.0 38.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4.0 3 5.0 4 2.0 5 1.0 6 2.0 7 2.0 8 2.0 9 2.0 10 2.0 11 0.0 12 1.0 13 2.0 14 2.0 15 1.0 16 2.0 17 7.0 18 4.0 19 3.0 20 3.0 21 2.0 22 4.0 23 12.0 24 6.0 25 5.0 26 15.0 27 12.0 28 14.0 29 20.0 30 36.0 31 39.0 32 48.0 33 66.0 34 106.0 35 170.0 36 397.0 37 3001.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 50.925 16.85 8.5 23.724999999999998 2 33.175 18.15 28.225 20.45 3 24.7 20.275000000000002 29.975 25.05 4 27.825 31.65 21.25 19.275000000000002 5 29.25 33.0 19.625 18.125 6 25.025 34.275 19.525000000000002 21.175 7 22.3 21.55 33.575 22.575 8 25.7 21.7 24.349999999999998 28.249999999999996 9 27.025 22.2 26.775 24.0 10-14 29.37 24.125 22.650000000000002 23.855 15-19 28.815 25.495 23.125 22.564999999999998 20-24 28.555000000000003 24.915000000000003 23.86 22.67 25-29 28.804999999999996 25.665 22.985 22.545 30-34 28.26 25.95 23.52 22.27 35-39 28.365000000000002 26.634999999999998 22.965 22.035 40-44 28.51 26.1 22.770000000000003 22.62 45-49 27.48 27.105 22.34 23.075000000000003 50-54 28.299999999999997 25.455 23.47 22.775000000000002 55-59 27.99 26.345000000000002 23.54 22.125 60-64 28.585 24.685000000000002 23.995 22.735 65-69 28.89 25.405 22.720000000000002 22.985 70-74 29.549999999999997 24.485 23.385 22.58 75-79 28.98 24.709999999999997 23.13 23.18 80-84 28.925 24.87 23.285 22.919999999999998 85-89 28.485 24.94 22.7 23.875 90-94 28.29 25.415 22.884999999999998 23.41 95-99 28.63 25.835 23.18 22.355 100-104 28.42 26.245 23.635 21.7 105-109 29.84798479847985 23.84738473847385 24.69246924692469 21.61216121612161 110-114 28.86644332216611 25.526276313815693 23.161158057902895 22.446122306115306 115-119 29.145 25.290000000000003 23.03 22.535 120-124 28.96 26.235000000000003 21.845 22.96 125-129 28.21 26.26 22.185 23.345 130-134 29.294999999999998 25.855 22.264999999999997 22.585 135-139 29.145 25.71 23.580000000000002 21.565 140-144 29.816490824541226 25.306265313265662 23.061153057652884 21.816090804540227 145-149 29.92248062015504 26.16154038509627 22.9057264316079 21.010252563140785 150-151 29.327163581790895 25.812906453226613 22.648824412206103 22.211105552776388 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.5 13 0.5 14 0.0 15 0.0 16 0.0 17 0.0 18 0.5 19 1.0 20 0.5 21 0.5 22 0.5 23 0.5 24 0.5 25 2.0 26 4.0 27 7.0 28 7.0 29 7.0 30 11.5 31 12.0 32 13.0 33 13.0 34 19.0 35 33.5 36 39.5 37 55.0 38 72.5 39 83.0 40 94.0 41 93.5 42 96.5 43 106.5 44 105.0 45 91.0 46 98.5 47 109.5 48 93.5 49 102.0 50 122.5 51 142.5 52 136.5 53 165.5 54 264.5 55 315.5 56 275.5 57 202.5 58 178.5 59 187.5 60 149.0 61 97.5 62 80.0 63 55.5 64 36.0 65 24.5 66 19.0 67 28.0 68 33.0 69 21.5 70 18.0 71 16.0 72 9.0 73 9.0 74 8.5 75 5.5 76 5.0 77 5.0 78 7.5 79 6.0 80 0.5 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.5 87 0.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.01 110-114 0.005 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.005 145-149 0.025 150-151 0.05 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 66.825 #Duplication Level Percentage of deduplicated Percentage of total 1 76.43097643097643 51.075 2 13.505424616535727 18.05 3 5.349794238683128 10.725 4 1.6835016835016834 4.5 5 1.122334455667789 3.75 6 0.823045267489712 3.3000000000000003 7 0.22446689113355783 1.05 8 0.18705574261129815 1.0 9 0.18705574261129815 1.125 >10 0.4863449307893753 5.425 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTTCGATCCTGGCTCAGGATGAACGCTGGCGGCATGCTTAACACATGCAA 34 0.8500000000000001 No Hit GGCTTGCGGTGGATACCTAGGCACCCAGAGACGAGGAAGGGCGTAGCAAG 32 0.8 No Hit GTGACCAGGCCCGGGCGACTGTTTACCAAAAACACAGGTCTCCGCAAAGT 19 0.475 No Hit GTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGATCTT 16 0.4 No Hit CCTGCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGCAA 16 0.4 No Hit GGCGGGACTACCCGCTGAGTTTAAGCATATAAATAAGCGGAGGAGAAGAA 15 0.375 No Hit GTCTGACATGCGTGCGAGTCGACGGGTTCTGAAACCTGGGATGCGCAAGG 14 0.35000000000000003 No Hit CCTGGCTCAGGATGAACGCTGGCGGCATGCTTAACACATGCAAGTCGAAC 13 0.325 No Hit ATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAA 12 0.3 No Hit CCTTACCAGGGCTTGACATGCCGCGAATCCTCTTGAAAGAGAGGGGTGCC 12 0.3 No Hit GCTAACTCCAAAAACCCGTCCTCAGTTCGGATTGCAGGCTGCAACTCGCC 12 0.3 No Hit GGGAGCTTGACTGCAAGACTCACCCGTCGAGCAGAGACGAAAGTCGGCCT 11 0.27499999999999997 No Hit GCTTGCGGTGGATACCTAGGCACCCAGAGACGAGGAAGGGCGTAGCAAGC 11 0.27499999999999997 No Hit GTCAGGCGGGACTACCCGCTGAGTTTAAGCATATAAATAAGCGGAGGAGA 9 0.22499999999999998 No Hit ACCAGGTCCAGACATAGCAAGGATTGACAGACTGAGAGCTCTTTCTTGAT 9 0.22499999999999998 No Hit GCCGCGAATCCTCTTGAAAGAGAGGGGTGCCCTCGGGAACGCGGACACAG 9 0.22499999999999998 No Hit GGAAGGCCTACGGGTCGTCAACTTCTTTTCTCGGAGAAGAAACAATGACG 9 0.22499999999999998 No Hit AGTGACCAGGCCCGGGCGACTGTTTACCAAAAACACAGGTCTCCGCAAAG 9 0.22499999999999998 No Hit GCTTGTTATATGGGTCGTGAGTGGGAACTTAGTTTCCGTTTGGGTATGCG 8 0.2 No Hit GGAGTCTGACATGCGTGCGAGTCGACGGGTTCTGAAACCTGGGATGCGCA 8 0.2 No Hit ATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCA 8 0.2 No Hit GCTTAACACATGCAAGTCGAACGGGAAGTGGTGTTTCCAGTGGCGAACGG 8 0.2 No Hit GCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGCAAGTA 8 0.2 No Hit CAGGGCTTGACATGCCGCGAATCCTCTTGAAAGAGAGGGGTGCCCTCGGG 7 0.17500000000000002 No Hit GTGCTGAGCTCCCAAGCAGTGGGAGGGGAAAGTGATCTCTGACCGCGTGC 7 0.17500000000000002 No Hit GTTGATAGTCAAGGTCGCGTTATTAATACTTGGGCTGATATCATCAACCG 7 0.17500000000000002 No Hit CGAAAACATTGGTGAGAATCCAATGCCCCGAAAACCCAAGGTTTCCTCCG 7 0.17500000000000002 No Hit GTGGATACCTAGGCACCCAGAGACGAGGAAGGGCGTAGCAAGCGACGAAA 7 0.17500000000000002 No Hit GGCTTTTCAAGTCCGCCGTCAAATCCCAGGGCTCAACCCTGGACAGGCGG 7 0.17500000000000002 No Hit GGCTGATCTTCCCCAAGAGTCCACATCGACGGGAAGGTTTGGCACCTCGA 6 0.15 No Hit GTTAAGTCTCGCAACGAGCGCAACCCTCGTGTTTAGTTGCCACTATGAGT 6 0.15 No Hit GGATGAACGCTGGCGGCATGCTTAACACATGCAAGTCGAACGGGAAGTGG 6 0.15 No Hit CTGGAATCGGTTCAGCCGGAGGTAGGGTCCAGTGGCCGGAAGAGCACCGC 6 0.15 No Hit CTTGCGGTGGATACCTAGGCACCCAGAGACGAGGAAGGGCGTAGCAAGCG 6 0.15 No Hit GGGAATTTCCGGTGGAGCGGTGAAATGCATTGAGATCGGAAAGAACACCA 6 0.15 No Hit CAGCCACACTGGGACTGAGACACGGCCCAGACTCCTACGGGAGGCAGCAG 6 0.15 No Hit AGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGATCT 6 0.15 No Hit GAGAAATCCGCCCAAGGAGGGGCTCGCGTCTGATTAGCTAGTTGGTGAGG 6 0.15 No Hit GGAACAACAACTGGAAACGGTTGCTAATACCCCGTAGGCTGAGGAGCAAA 6 0.15 No Hit GTTATTTTGGCCGATTAATCTTCCAATATGCTAGTTTCAACAACTCTCGT 6 0.15 No Hit CTCGGGAACGCGGACACAGGTGGTGCATGGCTGTCGTCAGCTCGTGCCGT 6 0.15 No Hit CGGACATTGGTCCTCGAGTGCAAAGGCAGAAGGGAGCTTGACTGCAAGAC 6 0.15 No Hit GTCAAAATGCATCGGTAGGGGAGCGTTCCGCCTTAGAGGGAAGCAACCGC 6 0.15 No Hit GACAGGTTAGTTTTACCCTACTGATGACCGTGCCGCGATAGTAATTCAAC 6 0.15 No Hit CTTACCAGGTCCAGACATAGCAAGGATTGACAGACTGAGAGCTCTTTCTT 6 0.15 No Hit ATCCTGGCTCAGGATGAACGCTGGCGGCATGCTTAACACATGCAAGTCGA 6 0.15 No Hit GCTAACTCTGTGCCAGCAGCCGCGGTAAGACAGAGGATGCAAGCGTTATC 6 0.15 No Hit GGATGATCAGCCACACTGGGACTGAGACACGGCCCAGACTCCTACGGGAG 6 0.15 No Hit GCACAACATCCTTATGCATCCATTTCACATGTTAGGTGTAGCTGGTGTTT 6 0.15 No Hit GTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGATCTTACCATGAC 6 0.15 No Hit GTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGATCTTACCATGACTG 6 0.15 No Hit GGTCGCTTCTGCAACTGGATAACTAGCACTGAAAATCGTCTTTACATCGG 5 0.125 No Hit GAACCTTACCAGGGCTTGACATGCCGCGAATCCTCTTGAAAGAGAGGGGT 5 0.125 No Hit CCTAAATGGATTCAATTTCAACCAATCTGTAGTTGATAGTCAAGGTCGCG 5 0.125 No Hit GGGAAGCAACCGCGAAAGCGGGGGTCGACGAAGCGGAAGCGAGAATGTCG 5 0.125 No Hit GGAGGGGCTCGCGTCTGATTAGCTAGTTGGTGAGGCAATAGCTTACCAAG 5 0.125 No Hit CCGTAAACGATGGATACTAGGTGCTGTGCGACTCGACCCGTGCAGTGCTG 5 0.125 No Hit CTGCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGCAAG 5 0.125 No Hit ATTTGGTCAAGAGGAAGAGACTTATAATATTGTGGCTGCTCATGGTTATT 5 0.125 No Hit GATGAACGCTGGCGGCATGCTTAACACATGCAAGTCGAACGGGAAGTGGT 5 0.125 No Hit GGTTAAGTCTCGCAACGAGCGCAACCCTCGTGTTTAGTTGCCACTATGAG 5 0.125 No Hit GGCCTCCCAAAAGGTAACGGAGGCGTGCAAAGGTTTCCTCGGGCCAGACG 5 0.125 No Hit CTTACCAGGGCTTGACATGCCGCGAATCCTCTTGAAAGAGAGGGGTGCCC 5 0.125 No Hit GGGGAATCCGACTGTTTAATTAAAACAAAGCATTGCGATGGTCCTCGCGG 5 0.125 No Hit GTTTGAGGCAATAACAGGTCTGTGATGCCCTTAGATGTTCTGGGCCGCAC 5 0.125 No Hit GGAAGCTTCTCTGATGGTATGCCTTTAGGAATATCTGGTACTTTCAACTT 5 0.125 No Hit GGACTACCCGCTGAGTTTAAGCATATAAATAAGCGGAGGAGAAGAAACTT 5 0.125 No Hit GGGAAGTGGTGTTTCCAGTGGCGAACGGGTGAGTAACGCGTAAGAACCTG 5 0.125 No Hit ATTCAGTGCTATGCATGGTTCCTTGGTAACCTCTAGTTTGATCAGGGAAA 5 0.125 No Hit GGGTGATCTATCCATGACCAGGATGAAGCTTGGATGAAACTAAGCAGAGG 5 0.125 No Hit CATCAACCGTGCTAATCTTGGTATGGAAGTAATGCACGAACGTAATGCTC 5 0.125 No Hit CAGTGACCAGGCCCGGGCGACTGTTTACCAAAAACACAGGTCTCCGCAAA 5 0.125 No Hit ACCTTACCAGGGCTTGACATGCCGCGAATCCTCTTGAAAGAGAGGGGTGC 5 0.125 No Hit GAGAAAATGCCTCGAGCCGAGGTCCGAGTACCAAGCGCTGCAGCGCTGAA 5 0.125 No Hit GGCGACTGTTTACCAAAAACACAGGTCTCCGCAAAGTCGTAAGACCATGT 5 0.125 No Hit CAAGCGTTGTGCTGCTAGGCGAAGCGGTTGAGTGCCGCACCCTAGATGGC 5 0.125 No Hit GTTCAACCTAAATGGATTCAATTTCAACCAATCTGTAGTTGATAGTCAAG 5 0.125 No Hit GTTCCGACCCGCACGAAAGGCGTAACGATCTGGGCACTGTCTCGGAGAGA 5 0.125 No Hit CAGCAAGGACCACCTTGCAAGGCTAAATACTCCTGGGTGACCGATAGCGA 5 0.125 No Hit GCGAAATTCCTTGTCGGGTAAGTTCCGACCCGCACGAAAGGCGTAACGAT 5 0.125 No Hit GTCTGTGATGCCCTTAGATGTTCTGGGCCGCACGCGCGCTACACTGATGT 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0125 0.0 0.0 0.0 0.0 68-69 0.025 0.0 0.0 0.0 0.0 70-71 0.025 0.0 0.0 0.0 0.0 72-73 0.075 0.0 0.0 0.0 0.0 74-75 0.1 0.0 0.0 0.0 0.0 76-77 0.175 0.0 0.0 0.0 0.0 78-79 0.175 0.0 0.0 0.0 0.0 80-81 0.2625 0.0 0.0 0.0 0.0 82-83 0.3375 0.0 0.0 0.0 0.0 84-85 0.3875 0.0 0.0 0.0 0.0 86-87 0.5125 0.0 0.0 0.0 0.0 88-89 0.6499999999999999 0.0 0.0 0.0 0.0 90-91 0.9125 0.0 0.0 0.0 0.0 92-93 1.125 0.0 0.0 0.0 0.0 94-95 1.4125 0.0 0.0 0.0 0.0 96-97 1.625 0.0 0.0 0.0 0.0 98-99 2.0375 0.0 0.0 0.0 0.0 100-101 2.4000000000000004 0.0 0.0 0.0 0.0 102-103 2.75 0.0 0.0 0.0 0.0 104-105 3.2125 0.0 0.0 0.0 0.0 106-107 3.5375 0.0 0.0 0.0 0.0 108-109 3.9875 0.0 0.0 0.0 0.0 110-111 4.4 0.0 0.0 0.0 0.0 112-113 5.074999999999999 0.0 0.0 0.0 0.0 114-115 5.4875 0.0 0.0 0.0 0.0 116-117 5.95 0.0 0.0 0.0 0.0 118-119 6.6625 0.0 0.0 0.0 0.0 120-121 7.0875 0.0 0.0 0.0 0.0 122-123 7.8125 0.0 0.0 0.0 0.0 124-125 8.425 0.0 0.0 0.0 0.0 126-127 9.1 0.0 0.0 0.0 0.0 128-129 9.774999999999999 0.0 0.0 0.0 0.0 130-131 10.587499999999999 0.0 0.0 0.0 0.0 132-133 11.4125 0.0 0.0 0.0 0.0 134-135 11.95 0.0 0.0 0.0 0.0 136-137 12.8625 0.0 0.0 0.0 0.0 138-139 13.6125 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content pass >>END_MODULE Read 1031863 spots for SRR6941585.sra Written 1031863 spots for SRR6941585.sra Read 1031863 spots for SRR6941585.sra Written 1031863 spots for SRR6941585.sra Read 1031863 spots for SRR6941585.sra Written 1031863 spots for SRR6941585.sra Read 1031863 spots for SRR6941585.sra Written 1031863 spots for SRR6941585.sra Read 1031863 spots for SRR6941585.sra Written 1031863 spots for SRR6941585.sra Read 1031863 spots for SRR6941585.sra Written 1031863 spots for SRR6941585.sra Read 1031863 spots for SRR6941585.sra Written 1031863 spots for SRR6941585.sra Read 1031863 spots for SRR6941585.sra Written 1031863 spots for SRR6941585.sra Read 1031863 spots for SRR6941585.sra Written 1031863 spots for SRR6941585.sra Read 1031870 spots for SRR6941585.sra Written 1031870 spots for SRR6941585.sra Read 1031863 spots for SRR6941585.sra Written 1031863 spots for SRR6941585.sra Read 1031863 spots for SRR6941585.sra Written 1031863 spots for SRR6941585.sra Read 1031863 spots for SRR6941585.sra Written 1031863 spots for SRR6941585.sra Read 1031863 spots for SRR6941585.sra Written 1031863 spots for SRR6941585.sra Read 1031863 spots for SRR6941585.sra Written 1031863 spots for SRR6941585.sra Read 1031863 spots for SRR6941585.sra Written 1031863 spots for SRR6941585.sra Read 1031863 spots for SRR6941585.sra Written 1031863 spots for SRR6941585.sra Read 1031863 spots for SRR6941585.sra Written 1031863 spots for SRR6941585.sra Read 1031863 spots for SRR6941585.sra Written 1031863 spots for SRR6941585.sra Read 1031863 spots for SRR6941585.sra Written 1031863 spots for SRR6941585.sra SRR ids: ['SRR6941585.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_oflwd1ob SRR6941585.sra spots: 20637267 blocks: [[1, 1031863], [1031864, 2063726], [2063727, 3095589], [3095590, 4127452], [4127453, 5159315], [5159316, 6191178], [6191179, 7223041], [7223042, 8254904], [8254905, 9286767], [9286768, 10318630], [10318631, 11350493], [11350494, 12382356], [12382357, 13414219], [13414220, 14446082], [14446083, 15477945], [15477946, 16509808], [16509809, 17541671], [17541672, 18573534], [18573535, 19605397], [19605398, 20637267]] SRR6941585 file size 6971592 SRR6941585 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR6941585 SRR6941585_1.fastq SRR6941585_2.fastq Input file: SRR6941585_1.fastq Paired file: SRR6941585_2.fastq trimmed: SRR6941585-trimmed-pair1.fastq, SRR6941585-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Fri Dec 6 12:12:07 2024 >> started Fri Dec 6 12:12:30 2024 >> done (23.818s) 20637267 read pairs processed; of these: 14130 ( 0.07%) short read pairs filtered out after trimming by size control 12364 ( 0.06%) empty read pairs filtered out after trimming by size control 20610773 (99.87%) read pairs available; of these: 9868617 (47.88%) trimmed read pairs available after processing 10742156 (52.12%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 4 0.00% 19 3 0.00% 20 1 0.00% 21 3 0.00% 22 7 0.00% 23 5 0.00% 24 10 0.00% 25 7 0.00% 26 7 0.00% 27 12 0.00% 28 9 0.00% 29 3 0.00% 30 11 0.00% 31 15 0.00% 32 10 0.00% 33 13 0.00% 34 18 0.00% 35 11 0.00% 36 18 0.00% 37 23 0.00% 38 27 0.00% 39 27 0.00% 40 31 0.00% 41 39 0.00% 42 21 0.00% 43 56 0.00% 44 48 0.00% 45 37 0.00% 46 60 0.00% 47 69 0.00% 48 72 0.00% 49 96 0.00% 50 113 0.00% 51 145 0.00% 52 152 0.00% 53 154 0.00% 54 238 0.00% 55 251 0.00% 56 290 0.00% 57 330 0.00% 58 373 0.00% 59 400 0.00% 60 441 0.00% 61 602 0.00% 62 747 0.00% 63 864 0.00% 64 944 0.00% 65 1137 0.01% 66 1198 0.01% 67 1360 0.01% 68 1657 0.01% 69 1749 0.01% 70 2114 0.01% 71 2273 0.01% 72 2922 0.01% 73 3168 0.02% 74 3242 0.02% 75 3986 0.02% 76 4365 0.02% 77 4985 0.02% 78 5622 0.03% 79 6913 0.03% 80 7790 0.04% 81 8015 0.04% 82 9106 0.04% 83 10871 0.05% 84 11210 0.05% 85 13859 0.07% 86 14802 0.07% 87 15694 0.08% 88 17809 0.09% 89 18568 0.09% 90 19757 0.10% 91 21163 0.10% 92 24462 0.12% 93 25500 0.12% 94 25820 0.13% 95 29855 0.14% 96 29815 0.14% 97 32961 0.16% 98 33614 0.16% 99 36509 0.18% 100 37796 0.18% 101 42117 0.20% 102 39955 0.19% 103 40644 0.20% 104 42882 0.21% 105 43061 0.21% 106 44536 0.22% 107 47232 0.23% 108 51712 0.25% 109 55098 0.27% 110 53938 0.26% 111 55865 0.27% 112 57995 0.28% 113 55634 0.27% 114 59885 0.29% 115 63549 0.31% 116 65312 0.32% 117 62631 0.30% 118 64435 0.31% 119 63806 0.31% 120 70779 0.34% 121 72762 0.35% 122 75571 0.37% 123 80460 0.39% 124 78310 0.38% 125 82887 0.40% 126 82081 0.40% 127 83752 0.41% 128 81857 0.40% 129 87082 0.42% 130 82923 0.40% 131 89732 0.44% 132 89392 0.43% 133 89628 0.43% 134 93000 0.45% 135 93291 0.45% 136 98842 0.48% 137 97668 0.47% 138 106736 0.52% 139 109660 0.53% 140 111365 0.54% 141 127629 0.62% 142 130550 0.63% 143 141711 0.69% 144 157122 0.76% 145 182903 0.89% 146 212461 1.03% 147 262281 1.27% 148 378517 1.84% 149 679608 3.30% 150 4229286 20.52% 151 10742156 52.12% 20610773 reads passed initial QC criterion=sequence-density sequence-density=3.12 sequence-density-rank=1 fanout-score=1.99 fanout-score-rank=25 prefix-density=3.09 prefix-fanout=2.0 sequence=GTATTTAGCCTTG criterion=fanout-score sequence-density=0.01 sequence-density-rank=35 fanout-score=59.76 fanout-score-rank=1 prefix-density=0.07 prefix-fanout=5.7 sequence=TTTTTATTTTGTTTCTTTTTATTTAGACCTTCTTCATATTTAGTTTTATCTATTAATCGATAGTATCTACCGGCGCGAACTCGAATTTGATCGCCTTCCATACTTCACAAGCTGCGGCTAGTTCAGGACTCCATTTGCAAGCTGCTCGGATAATTTCATTACCTTCACGAGCAAGATCGCGCCCTTCGTTACGAGCTTGTACACAGGCTTCTAAAGCCACTCGATTAGCTGCTGCACCAGGTGCATTTCCCCAAGGATGTCCTAAAGTTCCTCCACCAAATTGTAATACAGAATCATCCCCAAAGATTTCGGTCAGAGCTGGCATATGCCAAACATGAATACCACCTGAAGCTACTGGTATAACACCTGGCATGGATACCCAGTCCTGAGTGAAAAAGATACCGCGAGCACGATCTTTTTCAATAAAATCGTCGCGCAATAAATCAACAAAACCTAAAGTGATTTCGCGTTCCCCTTCTAACTTACCTACTACTGTACCGGCGTGGATATG criterion=sequence-density sequence-density=1.76 sequence-density-rank=1 fanout-score=2.07 fanout-score-rank=27 prefix-density=1.78 prefix-fanout=2.1 sequence=CCTAGTACGAGAGGA criterion=fanout-score sequence-density=0.02 sequence-density-rank=32 fanout-score=80.94 fanout-score-rank=1 prefix-density=1.24 prefix-fanout=1.1 sequence=AGAAGGGGTGCCCCCTCACAAAAGGGGGTCGCAGTGACCAGGCCCGGGCGACTGTTTACCAAAAACACAGGTCTCCGCAAAGTCGTAAGACCATGTATGGGGGCTGACGCCTGCCCAGTGCCGGAAGGTCAAGGAAGTTGGTGAACTGATGACAGGGAAGCCGGCGACCGAAGCCCCGGTGAACGGCGGCCGTAAC Potential 3prime adapter identified. Now checking if in reference sequence /dee2/code/volunteer_pipeline.sh: line 905: -f: command not found /dee2/code/volunteer_pipeline.sh: line 906: -f: command not found Adapter seq not found in reference. Now shuffling file before clipping skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -t 20 -x GTATTTAGCCTTG -y CCTAGTACGAGAGGA -o SRR6941585 SRR6941585_1.fastq SRR6941585_2.fastq Input file: SRR6941585_1.fastq Paired file: SRR6941585_2.fastq trimmed: SRR6941585-trimmed-pair1.fastq, SRR6941585-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): GTATTTAGCCTTG -- paired 3' end adapter sequence (-y): CCTAGTACGAGAGGA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Fri Dec 6 12:14:21 2024 >> started Fri Dec 6 12:14:29 2024 >> done (7.897s) 6870258 read pairs processed; of these: 236 ( 0.00%) short read pairs filtered out after trimming by size control 1165 ( 0.02%) empty read pairs filtered out after trimming by size control 6868857 (99.98%) read pairs available; of these: 1169 ( 0.02%) trimmed read pairs available after processing 6867688 (99.98%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 1 0.00% 19 0 0.00% 20 1 0.00% 21 0 0.00% 22 2 0.00% 23 2 0.00% 24 2 0.00% 25 1 0.00% 26 3 0.00% 27 3 0.00% 28 6 0.00% 29 1 0.00% 30 3 0.00% 31 10 0.00% 32 2 0.00% 33 1 0.00% 34 7 0.00% 35 5 0.00% 36 5 0.00% 37 4 0.00% 38 9 0.00% 39 9 0.00% 40 8 0.00% 41 12 0.00% 42 8 0.00% 43 19 0.00% 44 17 0.00% 45 17 0.00% 46 17 0.00% 47 22 0.00% 48 26 0.00% 49 22 0.00% 50 42 0.00% 51 44 0.00% 52 49 0.00% 53 55 0.00% 54 77 0.00% 55 85 0.00% 56 108 0.00% 57 117 0.00% 58 139 0.00% 59 129 0.00% 60 153 0.00% 61 191 0.00% 62 228 0.00% 63 278 0.00% 64 328 0.00% 65 401 0.01% 66 405 0.01% 67 470 0.01% 68 506 0.01% 69 590 0.01% 70 740 0.01% 71 714 0.01% 72 976 0.01% 73 1042 0.02% 74 1083 0.02% 75 1292 0.02% 76 1404 0.02% 77 1680 0.02% 78 1854 0.03% 79 2376 0.03% 80 2579 0.04% 81 2609 0.04% 82 3014 0.04% 83 3651 0.05% 84 3715 0.05% 85 4671 0.07% 86 4912 0.07% 87 5277 0.08% 88 5802 0.08% 89 6214 0.09% 90 6536 0.10% 91 7183 0.10% 92 8084 0.12% 93 8532 0.12% 94 8663 0.13% 95 10006 0.15% 96 10067 0.15% 97 11072 0.16% 98 11326 0.16% 99 12190 0.18% 100 12589 0.18% 101 14136 0.21% 102 13175 0.19% 103 13640 0.20% 104 14360 0.21% 105 14441 0.21% 106 14751 0.21% 107 15649 0.23% 108 17193 0.25% 109 18352 0.27% 110 17933 0.26% 111 18754 0.27% 112 19254 0.28% 113 18612 0.27% 114 19884 0.29% 115 21369 0.31% 116 21812 0.32% 117 20842 0.30% 118 21391 0.31% 119 21252 0.31% 120 23638 0.34% 121 24079 0.35% 122 25264 0.37% 123 26760 0.39% 124 26263 0.38% 125 27635 0.40% 126 27348 0.40% 127 28024 0.41% 128 27263 0.40% 129 29202 0.43% 130 27660 0.40% 131 29755 0.43% 132 29775 0.43% 133 29800 0.43% 134 30869 0.45% 135 30881 0.45% 136 33266 0.48% 137 32563 0.47% 138 35738 0.52% 139 36341 0.53% 140 37110 0.54% 141 42526 0.62% 142 43784 0.64% 143 47284 0.69% 144 52538 0.76% 145 61018 0.89% 146 70694 1.03% 147 87220 1.27% 148 126609 1.84% 149 226653 3.30% 150 1409057 20.51% 151 3578947 52.10% criterion=sequence-density sequence-density=3.13 sequence-density-rank=1 fanout-score=1.99 fanout-score-rank=27 prefix-density=3.10 prefix-fanout=2.0 sequence=GTATTTAGCCTTG criterion=fanout-score sequence-density=0.01 sequence-density-rank=35 fanout-score=105.79 fanout-score-rank=1 prefix-density=1.02 prefix-fanout=1.0 sequence=TTTGACATCCCCATGCCGCCACACCACAGGGGGGGGACATGGGGGCGTCAAAAAAGGGATCCTATCACTTATCAACTAATTTGTTCCGACCTAGGATAATAAGCTCATGAGCTTGGTCTTACTTCACCCTAAACGAAAGAAGACTTCCATATCCAAGTTTAGCTCAGACGTAGCTGCCTTCTTTTTGGGCGTGAAGCAGTGTCAAACCAAAATACCCAATAAGCATAAGCATTAGCTCTCCCTGAAAAGGAGGTGATCCAGCCGCACCTTCCAGTACGGCTACCTTGTTACGACTTCACTCCAGTCGCAAGCCTAGCCTTAGGCATCCCCCTCCTTACGGTTAAGGGTAATGACTTCAAACATGGCCAGCTCCTATAGTGTGACGGGCGGTGTGTACAAGGCCCGGGAACGGATTCACCGCCGTATGGCTGACCGGCGATTACTAGCGATTCCTGCTTCATGCAGGCGAGTTGCAGCCTGCAATCCGAACTGAGGACGGGTTTTTGGAGTT criterion=sequence-density sequence-density=1.78 sequence-density-rank=1 fanout-score=2.09 fanout-score-rank=25 prefix-density=1.80 prefix-fanout=2.1 sequence=CCTAGTACGAGAGGA criterion=fanout-score sequence-density=0.02 sequence-density-rank=31 fanout-score=80.03 fanout-score-rank=1 prefix-density=1.24 prefix-fanout=1.1 sequence=AGAAGGGGTGCCCCCTCACAAAAGGGGGTCGCAGTGACCAGGCCCGGGCGACTGTTTACCAAAAACACAGGTCTCCGCAAAGTCGTAAGACCATGTATGGGGGCTGACGCCTGCCCAGTGCCGGAAGGTCAAGGAAGTTGGTGAACTGATGACAGGGAAGCCGGCGACCGAAGCCCCGGTGAACGGCGGCCGTAAC SRR6941585 testing PE reads STAR mapping to Ensembl genome Started job on | Dec 06 12:15:30 Started mapping on | Dec 06 12:15:31 Finished on | Dec 06 12:17:12 Mapping speed, Million of reads per hour | 734.59 Number of input reads | 20609372 Average input read length | 290 UNIQUE READS: Uniquely mapped reads number | 8013523 Uniquely mapped reads % | 38.88% Average mapped length | 294.10 Number of splices: Total | 1004744 Number of splices: Annotated (sjdb) | 878027 Number of splices: GT/AG | 930893 Number of splices: GC/AG | 12416 Number of splices: AT/AC | 2904 Number of splices: Non-canonical | 58531 Mismatch rate per base, % | 0.22% Deletion rate per base | 0.01% Deletion average length | 1.95 Insertion rate per base | 0.01% Insertion average length | 2.37 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 7098562 % of reads mapped to multiple loci | 34.44% Number of reads mapped to too many loci | 763034 % of reads mapped to too many loci | 3.70% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 3.05% % of reads unmapped: other | 19.92% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 5503084 5503084 5503084 N_multimapping 7098562 7098562 7098562 N_noFeature 5398788 7873142 5458075 N_ambiguous 162717 2420 82916 UnstrandedReadsAssigned:2452018 PositiveStrandReadsAssigned:137961 NegativeStrandReadsAssigned:2472532 Dataset is classified negative stranded MeadianReadLen=151 20thPercentileLength=147 echo kmer=143 SRR6941585 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,972 [index] number of k-mers: 66,720,672 [index] number of equivalence classes: 111,837 [quant] running in paired-end mode [quant] will process pair 1: SRR6941585-trimmed-pair1.fastq SRR6941585-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 20,609,372 reads, 5,280,071 reads pseudoaligned [quant] estimated average fragment length: 203.131 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 971 rounds 52973 SRR6941585.ke.tsv 35125 SRR6941585.se.tsv 88098 total ==> SRR6941585.ke.tsv <== target_id length eff_length est_counts tpm PNS24245 936 734.116 1.33376 0.166917 PNS24247 1044 841.869 2.60433 0.284209 PNS24249 1928 1725.87 3.48044 0.185274 PNS24246 1044 841.869 2.60433 0.284209 PNS24248 1044 841.869 2.60433 0.284209 PNS24244 1471 1268.87 2.37281 0.171804 PNS24243 293 117.772 0 0 KQK14069 1603 1400.87 332.599 21.8127 KQK14071 474 279.705 5.53284 1.81733 ==> SRR6941585.se.tsv <== BRADI_1g14170v3 388 BRADI_1g53295v3 15 BRADI_1g59795v3 9 BRADI_1g07683v3 0 BRADI_1g00485v3 0 BRADI_1g20270v3 23 BRADI_1g74790v3 9 BRADI_1g09890v3 0 BRADI_1g77505v3 14 BRADI_1g48960v3 0 SRR6941585 completed mapping pipeline successfully