Starting /dee2/code/volunteer_pipeline.sh SRR6941586 current disk space = 1551495446528 free memory = 1607213480 SRR6941586 SRAfilesize d902140b69f816d8c83cbba389595585 SRR6941586.sra SRR6941586.sra file validated SRR6941586 is paired end SRR6941586 is conventional basespace SRR6941586 read1 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR6941586_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 50 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 24.889 27.0 18.0 32.0 18.0 33.0 2 24.3685 25.0 18.0 29.0 18.0 33.0 3 28.19725 29.0 27.0 31.0 18.0 33.0 4 30.75675 31.0 30.0 33.0 27.0 33.0 5 32.0385 33.0 32.0 33.0 31.0 33.0 6 35.97775 37.0 36.0 38.0 33.0 38.0 7 36.772 38.0 37.0 38.0 34.0 38.0 8 37.38525 38.0 38.0 38.0 37.0 38.0 9 37.57125 38.0 38.0 38.0 37.0 38.0 10-14 37.481849999999994 38.0 38.0 38.0 37.2 38.0 15-19 37.326299999999996 38.0 38.0 38.0 37.2 38.0 20-24 37.4477 38.0 38.0 38.0 37.2 38.0 25-29 37.541 38.0 38.0 38.0 37.8 38.0 30-34 37.5468 38.0 38.0 38.0 38.0 38.0 35-39 37.4992 38.0 38.0 38.0 37.6 38.0 40-44 37.4048 38.0 38.0 38.0 37.2 38.0 45-49 37.4 38.0 38.0 38.0 37.0 38.0 50-54 37.2386 38.0 38.0 38.0 36.6 38.0 55-59 37.1187 38.0 38.0 38.0 36.2 38.0 60-64 37.258300000000006 38.0 38.0 38.0 36.2 38.0 65-69 37.174249999999994 38.0 38.0 38.0 36.6 38.0 70-74 37.2007 38.0 38.0 38.0 36.4 38.0 75-79 37.10205 38.0 38.0 38.0 35.8 38.0 80-84 37.193200000000004 38.0 38.0 38.0 36.2 38.0 85-89 37.07405 38.0 38.0 38.0 35.8 38.0 90-94 36.070350000000005 38.0 37.0 38.0 29.6 38.0 95-99 36.617149999999995 38.0 37.8 38.0 34.4 38.0 100-104 36.934549999999994 38.0 38.0 38.0 35.0 38.0 105-109 36.76735 38.0 38.0 38.0 34.8 38.0 110-114 36.6721 38.0 38.0 38.0 34.6 38.0 115-119 36.51275 38.0 38.0 38.0 34.2 38.0 120-124 36.390950000000004 38.0 38.0 38.0 33.8 38.0 125-129 36.31529999999999 38.0 38.0 38.0 33.6 38.0 130-134 36.36655 38.0 38.0 38.0 34.0 38.0 135-139 35.96065 38.0 36.6 38.0 33.0 38.0 140-144 35.81955000000001 38.0 36.0 38.0 32.8 38.0 145-149 35.283699999999996 38.0 36.0 38.0 31.2 38.0 150-151 32.32475 37.0 33.5 38.0 15.0 38.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 5 1.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 1.0 14 0.0 15 1.0 16 1.0 17 0.0 18 0.0 19 2.0 20 2.0 21 1.0 22 1.0 23 0.0 24 8.0 25 4.0 26 6.0 27 10.0 28 22.0 29 26.0 30 44.0 31 60.0 32 65.0 33 95.0 34 129.0 35 242.0 36 674.0 37 2605.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 40.01065530101226 10.895045285029301 7.112413425679276 41.98188598827917 2 26.18154538634659 13.603400850212552 32.358089522380595 27.85696424106027 3 20.525 18.55 25.45 35.475 4 25.05 26.875 22.3 25.775 5 24.825 29.7 23.025000000000002 22.45 6 20.175 31.65 24.474999999999998 23.7 7 16.425 21.125 39.1 23.35 8 19.675 20.775 30.475 29.075 9 19.7 18.375 32.975 28.95 10-14 22.755 25.064999999999998 23.799999999999997 28.38 15-19 22.71 23.830000000000002 26.57 26.889999999999997 20-24 23.125 23.815 25.96 27.1 25-29 23.305 23.225 25.7 27.77 30-34 22.42 24.19 25.895000000000003 27.495000000000005 35-39 22.175 23.885 26.08 27.860000000000003 40-44 23.24 23.205000000000002 26.040000000000003 27.515 45-49 21.4 23.669999999999998 27.425 27.505000000000003 50-54 22.41 23.36 25.755 28.475 55-59 22.16 23.87 26.165 27.805000000000003 60-64 22.785 23.315 26.384999999999998 27.515 65-69 22.84 23.695 25.405 28.060000000000002 70-74 24.175 24.085 24.295 27.445000000000004 75-79 23.335 24.75 24.349999999999998 27.565 80-84 23.57 24.385 24.610000000000003 27.435 85-89 22.765 23.23 25.865 28.139999999999997 90-94 22.42 25.14 24.4 28.04 95-99 22.45 24.03 25.235000000000003 28.285 100-104 23.119999999999997 23.93 25.205 27.744999999999997 105-109 23.625 23.61 25.35 27.415 110-114 22.805 24.575 25.624999999999996 26.995 115-119 23.419999999999998 24.59 24.42 27.57 120-124 23.095 25.155 23.515 28.235 125-129 23.285 24.79 23.57 28.355000000000004 130-134 23.064999999999998 25.495 23.150000000000002 28.29 135-139 23.580000000000002 25.005 24.675 26.740000000000002 140-144 24.169999999999998 25.305 23.395 27.13 145-149 22.58 24.995 24.685000000000002 27.74 150-151 22.925 25.387500000000003 23.5625 28.125 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 1.0 21 1.5 22 1.5 23 1.5 24 2.0 25 3.5 26 5.0 27 9.5 28 12.0 29 11.5 30 14.5 31 16.0 32 14.0 33 12.0 34 16.0 35 22.5 36 41.5 37 85.5 38 90.0 39 80.0 40 103.0 41 110.5 42 102.5 43 109.0 44 117.0 45 111.5 46 118.5 47 112.0 48 90.0 49 91.0 50 120.0 51 142.0 52 139.0 53 152.0 54 207.0 55 285.5 56 293.0 57 242.0 58 211.5 59 185.5 60 138.5 61 96.0 62 67.0 63 43.5 64 31.5 65 22.0 66 14.5 67 10.5 68 11.0 69 9.0 70 13.0 71 16.0 72 14.0 73 9.5 74 4.5 75 6.5 76 5.5 77 2.0 78 1.0 79 0.0 80 0.5 81 0.5 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content warn #Base N-Count 1 6.15 2 0.025 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 72.2 #Duplication Level Percentage of deduplicated Percentage of total 1 81.4404432132964 58.8 2 10.837950138504155 15.65 3 3.4972299168975067 7.575 4 1.662049861495845 4.8 5 0.8310249307479225 3.0 6 0.7271468144044322 3.15 7 0.24238227146814403 1.225 8 0.24238227146814403 1.4000000000000001 9 0.13850415512465375 0.8999999999999999 >10 0.3808864265927978 3.5000000000000004 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCATTAAT 24 0.6 No Hit GTACAAGGCCCGGGAACGGATTCACCGCCGTATGGCTGACCGGCGATTAC 17 0.42500000000000004 No Hit GTGTACAAGGCCCGGGAACGGATTCACCGCCGTATGGCTGACCGGCGATT 13 0.325 No Hit GGGCTTACTACTTATATGCTTTCAGCAGTTATCCTCTCCGCACTTGGCTA 12 0.3 No Hit ACATGCTCCACCGCTTGTGCGGGCCCCCGTCAATTCCTTTGAGTTTCATT 11 0.27499999999999997 No Hit GCCAGCTCCTATAGTGTGACGGGCGGTGTGTACAAGGCCCGGGAACGGAT 11 0.27499999999999997 No Hit GTTGAAACTAGCATATTGGAAGATTAATCGGCCAAAATAACCATGAGCAG 11 0.27499999999999997 No Hit ACCACATGCTCCACCGCTTGTGCGGGCCCCCGTCAATTCCTTTGAGTTTC 11 0.27499999999999997 No Hit CACCGCTTGTGCGGGCCCCCGTCAATTCCTTTGAGTTTCATTCTTGCGAA 10 0.25 No Hit GTACAAAGGGCAGGGACGTAGTCAACGCGAGCTGATGACTCGCGCTTACT 10 0.25 No Hit CCCACTGCTGCCTCCCGTAGGAGTCTGGGCCGTGTCTCAGTCCCAGTGTG 10 0.25 No Hit CCTAGATGTCCAGTCAACTGCTGCGCCTCAACGCATTTCGGGGAGAACCA 9 0.22499999999999998 No Hit CGGGAACGGATTCACCGCCGTATGGCTGACCGGCGATTACTAGCGATTCC 9 0.22499999999999998 No Hit GCGGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTACG 9 0.22499999999999998 No Hit GTTTACGGCTAGGACTACTGGGGTCTCTAATCCCATTTGCTCCCCTAGCT 9 0.22499999999999998 No Hit CGGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTACGT 8 0.2 No Hit CTTGTCCGTACCAGTTCTGAGTCGACTGTTCAGCGCTCGGGGAAAGCCCC 8 0.2 No Hit GGTGGGCTTACTACTTATATGCTTTCAGCAGTTATCCTCTCCGCACTTGG 8 0.2 No Hit TTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCATTAATA 8 0.2 No Hit GTGCTCTGCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTCCTA 8 0.2 No Hit GGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTACGTT 8 0.2 No Hit CTCCACGCGGCATTGCTCCGTCAGGCTTTCGCCCATTGCGGAAAATTCCC 8 0.2 No Hit GGCATGATGACTTGGCCTCATCCTCTCCTTCCTCCGGCTTAACACCGGCG 7 0.17500000000000002 No Hit CCACATGCTCCACCGCTTGTGCGGGCCCCCGTCAATTCCTTTGAGTTTCA 7 0.17500000000000002 No Hit CTCAGATACCGTCATTGTTTCTTCTCCGAGAAAAGAAGTTGACGACCCGT 7 0.17500000000000002 No Hit CTCAGTGTCAGTGTCGGCCCAGCAGAGTGCTTTCGCCGTTGGTGTTCTTT 7 0.17500000000000002 No Hit GCGGAAAAACCTTATCCATTAATAGCGGGAACTTCAAGAGCAGCTAGATC 7 0.17500000000000002 No Hit CCGGGAACGGATTCACCGCCGTATGGCTGACCGGCGATTACTAGCGATTC 7 0.17500000000000002 No Hit CTCGCGAATACCATCAATATCTACTGGAGGGGCTGCGATGAAGGCGATAA 7 0.17500000000000002 No Hit CTCCACCGCTTGTGCGGGCCCCCGTCAATTCCTTTGAGTTTCATTCTTGC 6 0.15 No Hit CTCGCCTGTATTTAGCCTTGGACGGAGTCTACCGCCCGATTTGGGCTGCA 6 0.15 No Hit CCTCAGCCTACGGGGTATTAGCAACCGTTTCCAGTTGTTGTTCCCCTCCC 6 0.15 No Hit GGAAAAACCTTATCCATTAATAGCGGGAACTTCAAGAGCAGCTAGATCTA 6 0.15 No Hit CGGCAATTTCAAGCACTCTTTGACTCTCTTTTCAAAGTCCTTTTCATCTT 6 0.15 No Hit CACATGCTCCACCGCTTGTGCGGGCCCCCGTCAATTCCTTTGAGTTTCAT 6 0.15 No Hit GTCAATTCCTTTGAGTTTCATTCTTGCGAACGTACTCCCCAGGCGGGATA 6 0.15 No Hit CTCATCTTGGGGTGGGCTTACTACTTATATGCTTTCAGCAGTTATCCTCT 6 0.15 No Hit GCTCCACCGCTTGTGCGGGCCCCCGTCAATTCCTTTGAGTTTCATTCTTG 6 0.15 No Hit GGCTGATCATCCTCTCGGACCAGCTACTGATCATCGCCTTGGTAAGCTAT 6 0.15 No Hit CGGAAAAACCTTATCCATTAATAGCGGGAACTTCAAGAGCAGCTAGATCT 6 0.15 No Hit GTCGAGTTATCATGAATCATCGGATCAGCGAGCAAAGCCCGCGTCAGCCT 6 0.15 No Hit CCCGTTTTCACGGTTTAGGCTGCTCCCATTTCGCTCGCCGCTACTACGGG 6 0.15 No Hit CTTTCCCTCACGGTACTACTTCGCTATCGGTCACCCAGGAGTATTTAGCC 6 0.15 No Hit GTTCCGTTCCCTTAACCAAGCCACTGCCTATGAGTCGCCGGCTCATTCTT 6 0.15 No Hit GTGCGACGTGGGGCTGGATCTCAGTGGATCGTGGCAGCAAGGCCACTCTG 6 0.15 No Hit CCGCATTAATGGGCGAACAGCCCAACCCTTGGAACCACCTACAGCTCCAG 6 0.15 No Hit CCCCACTGCTGCCTCCCGTAGGAGTCTGGGCCGTGTCTCAGTCCCAGTGT 6 0.15 No Hit CTCAGCCTACGGGGTATTAGCAACCGTTTCCAGTTGTTGTTCCCCTCCCA 6 0.15 No Hit CTTTCTTTTCCTCTGGCTACTAAGATGTTTCAGTTCGCCAGGTTGTCTCT 6 0.15 No Hit CTCTGCCCCTACCGTACTCCAGCTTGGTAGTTTCCACCGCCTGTCCAGGG 6 0.15 No Hit CTCTGTCTTACCGCGGCTGCTGGCACAGAGTTAGCCGATGCTTATTCCTC 5 0.125 No Hit CGCAGAAGTAGGAATAATAGCACCAGAGATAATATTGTTTCCATAAAGTA 5 0.125 No Hit GGGCGGTGTGTACAAGGCCCGGGAACGGATTCACCGCCGTATGGCTGACC 5 0.125 No Hit GGCCAAAATAACCATGAGCAGCCACAATATTATAAGTCTCTTCCTCTTGA 5 0.125 No Hit ACCGCTTGTGCGGGCCCCCGTCAATTCCTTTGAGTTTCATTCTTGCGAAC 5 0.125 No Hit CACGTGTGTCGCCCAGGGCATAAGGGGCATGATGACTTGGCCTCATCCTC 5 0.125 No Hit GTCGGTTTCGGGTACAGGTACCCTTTTGTTGAAGGTCGTTCGAGCTTTTC 5 0.125 No Hit CCCTCATTAGCAGATTCATTTTCAGTAGTTTCCCTGATCAAACTAGAGGT 5 0.125 No Hit CACTCATCTTGGGGTGGGCTTACTACTTATATGCTTTCAGCAGTTATCCT 5 0.125 No Hit CATGCTCCACCGCTTGTGCGGGCCCCCGTCAATTCCTTTGAGTTTCATTC 5 0.125 No Hit CAATCATTCCGGATAACGCTTGCATCCTCTGTCTTACCGCGGCTGCTGGC 5 0.125 No Hit CTTAAACCTATAACCATCTTTCGGCTAACCTAGCCTCCTCCGTCCCTCCG 5 0.125 No Hit GCCTGTTATCCCTAGAGTAACTTTTATCCGTTGAGCGACGGCCCTTCCAC 5 0.125 No Hit GGTTTTTGGAGTTAGCTCACCCTCGCGAGATCGCGACCCTTTGTCCCGCC 5 0.125 No Hit CCCTACCGTACTCCAGCTTGGTAGTTTCCACCGCCTGTCCAGGGTTGAGC 5 0.125 No Hit CTCATAAGGACCACCATTGTATAACCATTCATCAACGGATGCAGCTTCCC 5 0.125 No Hit GGCGGATTTCTCCTTTTGCTCCTCAGCCTACGGGGTATTAGCAACCGTTT 5 0.125 No Hit AGCACGTGTGTCGCCCAGGGCATAAGGGGCATGATGACTTGGCCTCATCC 5 0.125 No Hit GGCAATTTCAAGCACTCTTTGACTCTCTTTTCAAAGTCCTTTTCATCTTT 5 0.125 No Hit GGCAACTAAACACGAGGGTTGCGCTCGTTGCGAGACTTAACCCAACACCT 5 0.125 No Hit CTTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCAT 5 0.125 No Hit TAGATGTCCAGTCAACTGCTGCGCCTCAACGCATTTCGGGGAGAACCAGC 5 0.125 No Hit CGCGACCTTGACTATCAACTACAGATTGGTTGAAATTGAATCCATTTAGG 5 0.125 No Hit ATAACAAGCTACACCAAGTAAGAAGTGTAGAACAATTAGCTCATAAGGAC 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.025 0.0 0.0 0.0 0.0 62-63 0.05 0.0 0.0 0.0 0.0 64-65 0.05 0.0 0.0 0.0 0.0 66-67 0.075 0.0 0.0 0.0 0.0 68-69 0.125 0.0 0.0 0.0 0.0 70-71 0.16249999999999998 0.0 0.0 0.0 0.0 72-73 0.21250000000000002 0.0 0.0 0.0 0.0 74-75 0.2875 0.0 0.0 0.0 0.0 76-77 0.3375 0.0 0.0 0.0 0.0 78-79 0.4 0.0 0.0 0.0 0.0 80-81 0.4625 0.0 0.0 0.0 0.0 82-83 0.475 0.0 0.0 0.0 0.0 84-85 0.525 0.0 0.0 0.0 0.0 86-87 0.7 0.0 0.0 0.0 0.0 88-89 0.8375 0.0 0.0 0.0 0.0 90-91 1.05 0.0 0.0 0.0 0.0 92-93 1.2625 0.0 0.0 0.0 0.0 94-95 1.5375 0.0 0.0 0.0 0.0 96-97 1.7625 0.0 0.0 0.0 0.0 98-99 2.15 0.0 0.0 0.0 0.0 100-101 2.5125 0.0 0.0 0.0 0.0 102-103 2.9 0.0 0.0 0.0 0.0 104-105 3.2750000000000004 0.0 0.0 0.0 0.0 106-107 3.775 0.0 0.0 0.0 0.0 108-109 4.3125 0.0 0.0 0.0 0.0 110-111 5.074999999999999 0.0 0.0 0.0 0.0 112-113 5.575 0.0 0.0 0.0 0.0 114-115 6.199999999999999 0.0 0.0 0.0 0.0 116-117 6.8875 0.0 0.0 0.0 0.0 118-119 7.4125 0.0 0.0 0.0 0.0 120-121 8.075 0.0 0.0 0.0 0.0 122-123 8.725 0.0 0.0 0.0 0.0 124-125 9.525 0.0 0.0 0.0 0.0 126-127 10.475 0.0 0.0 0.0 0.0 128-129 11.3 0.0 0.0 0.0 0.0 130-131 12.25 0.0 0.0 0.0 0.0 132-133 13.2375 0.0 0.0 0.0 0.0 134-135 14.1875 0.0 0.0 0.0 0.0 136-137 14.95 0.0 0.0 0.0 0.0 138-139 15.5375 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTTACGG 10 0.006836113 144.9625 7 GCTTTCT 25 7.470885E-4 90.366234 1 CTTTTCT 25 8.7222434E-4 86.97751 6 TTTTCTT 25 8.7222434E-4 86.97751 7 CTTTCTT 25 8.7222434E-4 86.97751 2 TTTCTTT 30 0.0017991947 72.48125 3 TTTCTTC 30 0.0017991947 72.48125 8 TTCTTCA 45 1.0562863E-4 64.42778 9 TTCTTTT 40 0.005627093 54.360935 4 TCTTTTC 45 0.008966441 48.320835 5 AAAAATT 30 0.0014459731 24.160418 15-19 GCGGAAA 40 0.007666461 18.120312 30-34 CGGAAAA 40 0.007666461 18.120312 30-34 >>END_MODULE SRR6941586 read2 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR6941586_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 50 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.9545 33.0 33.0 34.0 32.0 34.0 2 33.08825 34.0 33.0 34.0 32.0 34.0 3 33.099 34.0 33.0 34.0 33.0 34.0 4 33.016 34.0 33.0 34.0 32.0 34.0 5 33.02475 34.0 33.0 34.0 32.0 34.0 6 37.2115 38.0 38.0 38.0 37.0 38.0 7 37.2795 38.0 38.0 38.0 37.0 38.0 8 37.2715 38.0 38.0 38.0 37.0 38.0 9 37.1735 38.0 38.0 38.0 37.0 38.0 10-14 36.930949999999996 38.0 38.0 38.0 35.6 38.0 15-19 37.0746 38.0 38.0 38.0 36.4 38.0 20-24 37.262 38.0 38.0 38.0 37.0 38.0 25-29 37.18435 38.0 38.0 38.0 36.8 38.0 30-34 36.807750000000006 38.0 38.0 38.0 35.6 38.0 35-39 36.58989999999999 38.0 38.0 38.0 34.4 38.0 40-44 37.10695 38.0 38.0 38.0 36.4 38.0 45-49 37.173 38.0 38.0 38.0 36.8 38.0 50-54 37.117149999999995 38.0 38.0 38.0 36.6 38.0 55-59 37.00725 38.0 38.0 38.0 36.2 38.0 60-64 37.0789 38.0 38.0 38.0 36.2 38.0 65-69 36.971050000000005 38.0 38.0 38.0 35.6 38.0 70-74 36.724000000000004 38.0 38.0 38.0 34.8 38.0 75-79 36.85674999999999 38.0 38.0 38.0 35.4 38.0 80-84 36.657650000000004 38.0 38.0 38.0 35.0 38.0 85-89 36.38785 38.0 37.8 38.0 33.8 38.0 90-94 36.7305 38.0 38.0 38.0 35.0 38.0 95-99 36.6023 38.0 38.0 38.0 34.4 38.0 100-104 35.58785 38.0 36.6 38.0 30.2 38.0 105-109 35.795950000000005 38.0 36.6 38.0 31.2 38.0 110-114 36.36095 38.0 38.0 38.0 34.0 38.0 115-119 36.305600000000005 38.0 38.0 38.0 34.0 38.0 120-124 35.861599999999996 38.0 37.2 38.0 32.4 38.0 125-129 35.562200000000004 38.0 36.8 38.0 31.0 38.0 130-134 35.53455 38.0 36.0 38.0 31.8 38.0 135-139 32.14275 37.0 27.8 38.0 20.6 38.0 140-144 33.49985 37.6 31.6 38.0 24.6 38.0 145-149 34.0878 38.0 34.8 38.0 26.2 38.0 150-151 29.83675 35.5 27.5 38.0 7.5 38.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 4.0 4 1.0 5 1.0 6 1.0 7 1.0 8 0.0 9 0.0 10 2.0 11 0.0 12 2.0 13 1.0 14 3.0 15 0.0 16 0.0 17 2.0 18 5.0 19 2.0 20 1.0 21 6.0 22 6.0 23 6.0 24 13.0 25 11.0 26 24.0 27 23.0 28 33.0 29 38.0 30 34.0 31 59.0 32 80.0 33 112.0 34 178.0 35 299.0 36 717.0 37 2332.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 42.3 15.8 10.85 31.05 2 31.407851962990748 17.079269817454364 30.282570642660666 21.230307576894223 3 23.760640961442164 21.382073109664496 31.071607411116673 23.785678517776667 4 28.853853853853856 29.47947947947948 23.04804804804805 18.61861861861862 5 31.38138138138138 31.956956956956954 17.992992992992992 18.66866866866867 6 24.575 34.325 19.875 21.224999999999998 7 23.1 18.3 35.099999999999994 23.5 8 25.05 22.85 24.675 27.425 9 26.075 22.075 25.874999999999996 25.974999999999998 10-14 28.31641582079104 24.8162408120406 22.916145807290363 23.951197559877993 15-19 28.435 25.53 24.08 21.955 20-24 28.04 25.259999999999998 24.22 22.48 25-29 28.221411070553525 25.531276563828193 23.416170808540425 22.831141557077853 30-34 27.722772277227726 25.752575257525752 24.352435243524354 22.172217221722175 35-39 28.247824782478247 26.062606260626065 23.692369236923692 21.997199719971995 40-44 27.93 26.11 23.28 22.68 45-49 27.99419912986948 25.978896834525177 23.158473771065662 22.86843026453968 50-54 28.461423071153558 25.281264063203164 23.641182059102956 22.616130806540326 55-59 27.57 25.755 23.880000000000003 22.795 60-64 28.987898789878987 24.857485748574856 24.392439243924393 21.76217621762176 65-69 28.10921638245737 25.54383157473621 23.728559283892583 22.61839275891384 70-74 28.954343151472724 24.818722808421263 24.178626794019102 22.048307246086914 75-79 28.816440822041102 24.036201810090503 24.24121206060303 22.906145307265362 80-84 28.71643582179109 24.866243312165608 24.056202810140505 22.361118055902796 85-89 28.294999999999998 25.285000000000004 23.485 22.935 90-94 27.615000000000002 26.08 23.155 23.150000000000002 95-99 28.48 25.345000000000002 23.745 22.43 100-104 27.74138706935347 26.116305815290765 24.321216060803042 21.821091054552728 105-109 28.73 24.505 24.86 21.905 110-114 27.69638481924096 25.631281564078208 23.701185059252964 22.97114855742787 115-119 28.88644432221611 25.716285814290714 23.231161558077904 22.16610830541527 120-124 28.351417570878546 26.541327066353315 22.211110555527778 22.89614480724036 125-129 29.566478323916197 25.67128356417821 22.086104305215258 22.676133806690334 130-134 29.315 26.450000000000003 22.37 21.865000000000002 135-139 28.765 25.855 23.755000000000003 21.625 140-144 30.036501825091257 26.01130056502825 22.87114355717786 21.081054052702637 145-149 29.635 25.865 23.175 21.325 150-151 30.9375 26.3 22.075 20.6875 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.5 21 2.0 22 2.5 23 2.5 24 3.5 25 4.5 26 4.5 27 4.5 28 10.0 29 14.5 30 17.5 31 17.5 32 15.0 33 17.5 34 24.5 35 34.0 36 48.0 37 60.0 38 66.0 39 95.0 40 112.0 41 102.0 42 103.0 43 108.5 44 117.0 45 101.0 46 88.0 47 101.0 48 98.5 49 90.5 50 99.5 51 116.5 52 128.5 53 174.5 54 253.5 55 301.0 56 280.5 57 213.5 58 164.5 59 164.0 60 146.0 61 113.0 62 100.0 63 65.5 64 35.0 65 23.0 66 15.0 67 21.5 68 28.5 69 21.0 70 12.5 71 8.0 72 6.5 73 7.0 74 6.0 75 6.5 76 5.5 77 7.5 78 7.5 79 2.5 80 0.5 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.025 3 0.15 4 0.1 5 0.1 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.005 15-19 0.0 20-24 0.0 25-29 0.005 30-34 0.01 35-39 0.01 40-44 0.0 45-49 0.015 50-54 0.005 55-59 0.0 60-64 0.01 65-69 0.015 70-74 0.015 75-79 0.005 80-84 0.005 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.005 105-109 0.0 110-114 0.005 115-119 0.005 120-124 0.005 125-129 0.005 130-134 0.0 135-139 0.0 140-144 0.005 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 71.0 #Duplication Level Percentage of deduplicated Percentage of total 1 79.8943661971831 56.725 2 11.725352112676056 16.650000000000002 3 4.014084507042254 8.55 4 2.147887323943662 6.1 5 0.8450704225352111 3.0 6 0.45774647887323944 1.95 7 0.24647887323943662 1.225 8 0.17605633802816903 1.0 9 0.17605633802816903 1.125 >10 0.31690140845070425 3.675 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTTCGATCCTGGCTCAGGATGAACGCTGGCGGCATGCTTAACACATGCAA 38 0.95 No Hit CCTTACCAGGGCTTGACATGCCGCGAATCCTCTTGAAAGAGAGGGGTGCC 23 0.575 No Hit GGCTTGCGGTGGATACCTAGGCACCCAGAGACGAGGAAGGGCGTAGCAAG 18 0.44999999999999996 No Hit GTGACCAGGCCCGGGCGACTGTTTACCAAAAACACAGGTCTCCGCAAAGT 14 0.35000000000000003 No Hit CCTGGCTCAGGATGAACGCTGGCGGCATGCTTAACACATGCAAGTCGAAC 11 0.27499999999999997 No Hit GCTTGCGGTGGATACCTAGGCACCCAGAGACGAGGAAGGGCGTAGCAAGC 11 0.27499999999999997 No Hit GCTTGACATGCCGCGAATCCTCTTGAAAGAGAGGGGTGCCCTCGGGAACG 11 0.27499999999999997 No Hit GGATGATCAGCCACACTGGGACTGAGACACGGCCCAGACTCCTACGGGAG 11 0.27499999999999997 No Hit AGTGACCAGGCCCGGGCGACTGTTTACCAAAAACACAGGTCTCCGCAAAG 10 0.25 No Hit ACCAGGTCCAGACATAGCAAGGATTGACAGACTGAGAGCTCTTTCTTGAT 9 0.22499999999999998 No Hit CTTACCAGGGCTTGACATGCCGCGAATCCTCTTGAAAGAGAGGGGTGCCC 9 0.22499999999999998 No Hit GACCAGGCCCGGGCGACTGTTTACCAAAAACACAGGTCTCCGCAAAGTCG 9 0.22499999999999998 No Hit CCTGAACAGACCGCCGGTGTTAAGCCGGAGGAAGGAGAGGATGAGGCCAA 9 0.22499999999999998 No Hit GCTTAACACATGCAAGTCGAACGGGAAGTGGTGTTTCCAGTGGCGAACGG 9 0.22499999999999998 No Hit GGCTGATCTTCCCCAAGAGTCCACATCGACGGGAAGGTTTGGCACCTCGA 8 0.2 No Hit CCTGCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGCAA 8 0.2 No Hit CGGACATTGGTCCTCGAGTGCAAAGGCAGAAGGGAGCTTGACTGCAAGAC 8 0.2 No Hit CGCGAATCCTCTTGAAAGAGAGGGGTGCCCTCGGGAACGCGGACACAGGT 8 0.2 No Hit GCTAACTCCAAAAACCCGTCCTCAGTTCGGATTGCAGGCTGCAACTCGCC 8 0.2 No Hit GTTAAGTCTCGCAACGAGCGCAACCCTCGTGTTTAGTTGCCACTATGAGT 7 0.17500000000000002 No Hit CTTGCGGTGGATACCTAGGCACCCAGAGACGAGGAAGGGCGTAGCAAGCG 7 0.17500000000000002 No Hit CTATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAA 7 0.17500000000000002 No Hit GTCTGACATGCGTGCGAGTCGACGGGTTCTGAAACCTGGGATGCGCAAGG 7 0.17500000000000002 No Hit GGAAGCTTCTCTGATGGTATGCCTTTAGGAATATCTGGTACTTTCAACTT 7 0.17500000000000002 No Hit ATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCA 7 0.17500000000000002 No Hit CAGTGACCAGGCCCGGGCGACTGTTTACCAAAAACACAGGTCTCCGCAAA 7 0.17500000000000002 No Hit CAGGGCTTGACATGCCGCGAATCCTCTTGAAAGAGAGGGGTGCCCTCGGG 6 0.15 No Hit TTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAA 6 0.15 No Hit CTGCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGCAAG 6 0.15 No Hit GCTTGTTATATGGGTCGTGAGTGGGAACTTAGTTTCCGTTTGGGTATGCG 6 0.15 No Hit CTGACACTGAGAGACGAAAGCTAGGGGAGCAAATGGGATTAGAGACCCCA 6 0.15 No Hit CACACGTGCTACAATGGGCGGGACAAAGGGTCGCGATCTCGCGAGGGTGA 6 0.15 No Hit GCTGCGACTGCTGTTTTCTTGATTTACCCTATTGGTCAAGGAAGCTTCTC 6 0.15 No Hit CCTAGGCACCCAGAGACGAGGAAGGGCGTAGCAAGCGACGAAATGCTTCG 6 0.15 No Hit GGACAGTCTCAGGTAGACAGTTTCTATGGGGCGTAGGCCTCCCAAAAGGT 6 0.15 No Hit GGCAGAGGGAATTTCCGGTGGAGCGGTGAAATGCATTGAGATCGGAAAGA 6 0.15 No Hit TATCTATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTT 6 0.15 No Hit GGTACTTTCAACTTTATGATTGTATTCCAGGCAGAGCACAACATCCTTAT 6 0.15 No Hit GCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGCAAGTA 6 0.15 No Hit AGACAGAGGATGCAAGCGTTATCCGGAATGATTGGGCGTAAAGCGTCTGT 5 0.125 No Hit GTTGCTAATACCCCGTAGGCTGAGGAGCAAAAGGAGAAATCCGCCCAAGG 5 0.125 No Hit CTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGAT 5 0.125 No Hit CGGACACAGGTGGTGCATGGCTGTCGTCAGCTCGTGCCGTAAGGTGTTGG 5 0.125 No Hit GCTGGTCCGAGAGGATGATCAGCCACACTGGGACTGAGACACGGCCCAGA 5 0.125 No Hit GGAAGGGCCGTCGCTCAACGGATAAAAGTTACTCTAGGGATAACAGGCTG 5 0.125 No Hit GGGAATTTCCGGTGGAGCGGTGAAATGCATTGAGATCGGAAAGAACACCA 5 0.125 No Hit TGAAAATGAATCTGCTAATGAGGGTTACAAATTTGGTCAAGAGGAAGAGA 5 0.125 No Hit CGAGTATATAGCCTTGGCCGACAGGCCCGGGTAATCTTGGGAAATTTCAT 5 0.125 No Hit GGTTAAGTCTCGCAACGAGCGCAACCCTCGTGTTTAGTTGCCACTATGAG 5 0.125 No Hit CGGCTCTTCGCCACCTGGAGCTGTAGGTGGTTCCAAGGGTTGGGCTGTTC 5 0.125 No Hit GGAAGGCCTACGGGTCGTCAACTTCTTTTCTCGGAGAAGAAACAATGACG 5 0.125 No Hit ATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCTAAATGG 5 0.125 No Hit GCCGGAGGAAGGAGAGGATGAGGCCAAGTCATCATGCCCCTTATGCCCTG 5 0.125 No Hit CTTACCAGGTCCAGACATAGCAAGGATTGACAGACTGAGAGCTCTTTCTT 5 0.125 No Hit GGGTAAGTTCCGACCCGCACGAAAGGCGTAACGATCTGGGCACTGTCTCG 5 0.125 No Hit GTTGGGTTAAGTCTCGCAACGAGCGCAACCCTCGTGTTTAGTTGCCACTA 5 0.125 No Hit GTCAAGGTCGCGTTATTAATACTTGGGCTGATATCATCAACCGTGCTAAT 5 0.125 No Hit CGGCCAGTGAGACGATGGGGGATAAGCTTCATCGTCGAGAGGGAAACAGC 5 0.125 No Hit CGGGTGAGTAACGCGTAAGAACCTGCCCTTGGGAGGGGAACAACAACTGG 5 0.125 No Hit CGATCCTGGCTCAGGATGAACGCTGGCGGCATGCTTAACACATGCAAGTC 5 0.125 No Hit GTCAAATCCCAGGGCTCAACCCTGGACAGGCGGTGGAAACTACCAAGCTG 5 0.125 No Hit CTCGACCCGTGCAGTGCTGTAGCTAACGCGTTAAGTATCCCGCCTGGGGA 5 0.125 No Hit CCGCAAAGTCGTAAGACCATGTATGGGGGCTGACGCCTGCCCAGTGCCGG 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.025 0.0 0.0 0.0 0.0 62-63 0.05 0.0 0.0 0.0 0.0 64-65 0.05 0.0 0.0 0.0 0.0 66-67 0.075 0.0 0.0 0.0 0.0 68-69 0.125 0.0 0.0 0.0 0.0 70-71 0.16249999999999998 0.0 0.0 0.0 0.0 72-73 0.21250000000000002 0.0 0.0 0.0 0.0 74-75 0.2875 0.0 0.0 0.0 0.0 76-77 0.3375 0.0 0.0 0.0 0.0 78-79 0.4 0.0 0.0 0.0 0.0 80-81 0.4625 0.0 0.0 0.0 0.0 82-83 0.475 0.0 0.0 0.0 0.0 84-85 0.525 0.0 0.0 0.0 0.0 86-87 0.7125 0.0 0.0 0.0 0.0 88-89 0.8625 0.0 0.0 0.0 0.0 90-91 1.075 0.0 0.0 0.0 0.0 92-93 1.2625 0.0 0.0 0.0 0.0 94-95 1.525 0.0 0.0 0.0 0.0 96-97 1.6875 0.0 0.0 0.0 0.0 98-99 2.0375 0.0 0.0 0.0 0.0 100-101 2.3375000000000004 0.0 0.0 0.0 0.0 102-103 2.725 0.0 0.0 0.0 0.0 104-105 3.0999999999999996 0.0 0.0 0.0 0.0 106-107 3.575 0.0 0.0 0.0 0.0 108-109 4.112500000000001 0.0 0.0 0.0 0.0 110-111 4.875 0.0 0.0 0.0 0.0 112-113 5.375 0.0 0.0 0.0 0.0 114-115 5.9875 0.0 0.0 0.0 0.0 116-117 6.675 0.0 0.0 0.0 0.0 118-119 7.2125 0.0 0.0 0.0 0.0 120-121 7.875 0.0 0.0 0.0 0.0 122-123 8.525 0.0 0.0 0.0 0.0 124-125 9.2625 0.0 0.0 0.0 0.0 126-127 10.075 0.0 0.0 0.0 0.0 128-129 10.774999999999999 0.0 0.0 0.0 0.0 130-131 11.5375 0.0 0.0 0.0 0.0 132-133 12.3125 0.0 0.0 0.0 0.0 134-135 12.975 0.0 0.0 0.0 0.0 136-137 13.5875 0.0 0.0 0.0 0.0 138-139 14.149999999999999 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content pass >>END_MODULE Read 1005791 spots for SRR6941586.sra Written 1005791 spots for SRR6941586.sra Read 1005791 spots for SRR6941586.sra Written 1005791 spots for SRR6941586.sra Read 1005791 spots for SRR6941586.sra Written 1005791 spots for SRR6941586.sra Read 1005791 spots for SRR6941586.sra Written 1005791 spots for SRR6941586.sra Read 1005791 spots for SRR6941586.sra Written 1005791 spots for SRR6941586.sra Read 1005791 spots for SRR6941586.sra Written 1005791 spots for SRR6941586.sra Read 1005791 spots for SRR6941586.sra Written 1005791 spots for SRR6941586.sra Read 1005791 spots for SRR6941586.sra Written 1005791 spots for SRR6941586.sra Read 1005791 spots for SRR6941586.sra Written 1005791 spots for SRR6941586.sra Read 1005791 spots for SRR6941586.sra Written 1005791 spots for SRR6941586.sra Read 1005791 spots for SRR6941586.sra Written 1005791 spots for SRR6941586.sra Read 1005791 spots for SRR6941586.sra Written 1005791 spots for SRR6941586.sra Read 1005791 spots for SRR6941586.sra Written 1005791 spots for SRR6941586.sra Read 1005791 spots for SRR6941586.sra Written 1005791 spots for SRR6941586.sra Read 1005791 spots for SRR6941586.sra Written 1005791 spots for SRR6941586.sra Read 1005791 spots for SRR6941586.sra Written 1005791 spots for SRR6941586.sra Read 1005791 spots for SRR6941586.sra Written 1005791 spots for SRR6941586.sra Read 1005791 spots for SRR6941586.sra Written 1005791 spots for SRR6941586.sra Read 1005791 spots for SRR6941586.sra Written 1005791 spots for SRR6941586.sra Read 1005801 spots for SRR6941586.sra Written 1005801 spots for SRR6941586.sra SRR ids: ['SRR6941586.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_88kda0of SRR6941586.sra spots: 20115830 blocks: [[1, 1005791], [1005792, 2011582], [2011583, 3017373], [3017374, 4023164], [4023165, 5028955], [5028956, 6034746], [6034747, 7040537], [7040538, 8046328], [8046329, 9052119], [9052120, 10057910], [10057911, 11063701], [11063702, 12069492], [12069493, 13075283], [13075284, 14081074], [14081075, 15086865], [15086866, 16092656], [16092657, 17098447], [17098448, 18104238], [18104239, 19110029], [19110030, 20115830]] SRR6941586 file size 6794894 SRR6941586 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR6941586 SRR6941586_1.fastq SRR6941586_2.fastq Input file: SRR6941586_1.fastq Paired file: SRR6941586_2.fastq trimmed: SRR6941586-trimmed-pair1.fastq, SRR6941586-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Fri Dec 6 12:14:34 2024 >> started Fri Dec 6 12:14:57 2024 >> done (22.557s) 20115830 read pairs processed; of these: 21443 ( 0.11%) short read pairs filtered out after trimming by size control 15710 ( 0.08%) empty read pairs filtered out after trimming by size control 20078677 (99.82%) read pairs available; of these: 8902160 (44.34%) trimmed read pairs available after processing 11176517 (55.66%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 2 0.00% 19 6 0.00% 20 3 0.00% 21 2 0.00% 22 7 0.00% 23 4 0.00% 24 0 0.00% 25 5 0.00% 26 1 0.00% 27 20 0.00% 28 5 0.00% 29 10 0.00% 30 12 0.00% 31 15 0.00% 32 9 0.00% 33 10 0.00% 34 12 0.00% 35 14 0.00% 36 10 0.00% 37 11 0.00% 38 21 0.00% 39 25 0.00% 40 26 0.00% 41 34 0.00% 42 48 0.00% 43 43 0.00% 44 50 0.00% 45 61 0.00% 46 64 0.00% 47 63 0.00% 48 90 0.00% 49 93 0.00% 50 128 0.00% 51 142 0.00% 52 195 0.00% 53 155 0.00% 54 235 0.00% 55 280 0.00% 56 316 0.00% 57 345 0.00% 58 411 0.00% 59 484 0.00% 60 572 0.00% 61 690 0.00% 62 756 0.00% 63 991 0.00% 64 1067 0.01% 65 1257 0.01% 66 1442 0.01% 67 1421 0.01% 68 1851 0.01% 69 1893 0.01% 70 2323 0.01% 71 2523 0.01% 72 3172 0.02% 73 3419 0.02% 74 3843 0.02% 75 4451 0.02% 76 5137 0.03% 77 5827 0.03% 78 6326 0.03% 79 7574 0.04% 80 8743 0.04% 81 9247 0.05% 82 10436 0.05% 83 12309 0.06% 84 13433 0.07% 85 15937 0.08% 86 16897 0.08% 87 18078 0.09% 88 20720 0.10% 89 21524 0.11% 90 23062 0.11% 91 24186 0.12% 92 26950 0.13% 93 28663 0.14% 94 29494 0.15% 95 33719 0.17% 96 34492 0.17% 97 38202 0.19% 98 39031 0.19% 99 41673 0.21% 100 42087 0.21% 101 46817 0.23% 102 45932 0.23% 103 46195 0.23% 104 49385 0.25% 105 50891 0.25% 106 52632 0.26% 107 55266 0.28% 108 60366 0.30% 109 61568 0.31% 110 61260 0.31% 111 62345 0.31% 112 63817 0.32% 113 63702 0.32% 114 66856 0.33% 115 70447 0.35% 116 72099 0.36% 117 70364 0.35% 118 72579 0.36% 119 71763 0.36% 120 77945 0.39% 121 79875 0.40% 122 80914 0.40% 123 86188 0.43% 124 83647 0.42% 125 89732 0.45% 126 87544 0.44% 127 88625 0.44% 128 87730 0.44% 129 92091 0.46% 130 89393 0.45% 131 93170 0.46% 132 94158 0.47% 133 93786 0.47% 134 97041 0.48% 135 98378 0.49% 136 103113 0.51% 137 100913 0.50% 138 107160 0.53% 139 108208 0.54% 140 108125 0.54% 141 121383 0.60% 142 122229 0.61% 143 128861 0.64% 144 139252 0.69% 145 160190 0.80% 146 180138 0.90% 147 215078 1.07% 148 296210 1.48% 149 535882 2.67% 150 3342032 16.64% 151 11176517 55.66% 20078677 reads passed initial QC criterion=sequence-density sequence-density=2.43 sequence-density-rank=1 fanout-score=1.98 fanout-score-rank=29 prefix-density=2.39 prefix-fanout=2.0 sequence=GTATTTAGCCTTG criterion=fanout-score sequence-density=0.03 sequence-density-rank=33 fanout-score=35.60 fanout-score-rank=1 prefix-density=0.96 prefix-fanout=1.1 sequence=TTTGACATCCCCATGCCGCCACACCACAGGGGGGGGACATGGGGGCGTCAAAAAAGGGATCCTATCACTTATCAACTAATTTGTTCCGACCTAGGATAATAAGCTCATGAGCTTGGTCTTACTTCACCCTAAACGAAAGAAGACTTCCATATCCAAGTTTAGCTCAGACGTAGCTGCCTTCTTTTTGGGCGTGAAGCAGTGTCAAACCAAAATACCCAATAAGCATAAGCATTAGCTCTCCCTGAAAAGGAGGTGATCCAGCCGCACCTTCCAGTACGGCTACCTTGTT criterion=sequence-density sequence-density=0.98 sequence-density-rank=1 fanout-score=6.72 fanout-score-rank=7 prefix-density=4.27 prefix-fanout=1.5 sequence=TGGTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGTTAACGAACGAGACCTCAGCCTGCTAACTAGCTATGCGGAGCCATCCCTCCGCAGCTAGCTTCTTAGAGGGACTATCGCCGTTTAGGCGACGGAAGTTTGAGGCAATAACAGGTCTGTGATGCCCTTAGATGTTCTGGGCCGCACGCGCGCTACACTGATGTATTCAACGAGTATATAGCCTTGGCCGACAGGCCCGGGTAATCTTGGGAAATTTCATCGTGATGGGGATAGATCATTGCAATTGTTGGTCTTCAACGAGGAATGCCTAGTAAGCGCGAGTCATCAGCTCGCGTTGACTACGTCCCTGCCCTTTGTACACACC criterion=fanout-score sequence-density=0.04 sequence-density-rank=32 fanout-score=49.34 fanout-score-rank=1 prefix-density=2.09 prefix-fanout=1.0 sequence=CGCGGTAAGACGGGGGGGGCAAGTGTTCTTCGGAATGACTGGGCGTAAAGGGCACGTAGGCGGTGAATCGGGTTGAAAGTGAAAGTCGCCAAAAAGTGGCGGAATGCTCTCGAAACCAATTCACTTGAGTGAGACAGAGGAGAGTGGAATTTCGTGTGTAGGGGTGAAA SRR6941586 testing PE reads STAR mapping to Ensembl genome Started job on | Dec 06 12:15:52 Started mapping on | Dec 06 12:15:52 Finished on | Dec 06 12:17:37 Mapping speed, Million of reads per hour | 688.41 Number of input reads | 20078677 Average input read length | 288 UNIQUE READS: Uniquely mapped reads number | 8280624 Uniquely mapped reads % | 41.24% Average mapped length | 293.56 Number of splices: Total | 1077321 Number of splices: Annotated (sjdb) | 969896 Number of splices: GT/AG | 1028205 Number of splices: GC/AG | 12899 Number of splices: AT/AC | 3434 Number of splices: Non-canonical | 32783 Mismatch rate per base, % | 0.17% Deletion rate per base | 0.01% Deletion average length | 1.59 Insertion rate per base | 0.01% Insertion average length | 1.68 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 7963214 % of reads mapped to multiple loci | 39.66% Number of reads mapped to too many loci | 572089 % of reads mapped to too many loci | 2.85% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 2.44% % of reads unmapped: other | 13.81% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 3840484 3840484 3840484 N_multimapping 7963214 7963214 7963214 N_noFeature 5275241 8107577 5339929 N_ambiguous 223413 3274 118479 UnstrandedReadsAssigned:2781970 PositiveStrandReadsAssigned:169773 NegativeStrandReadsAssigned:2822216 Dataset is classified negative stranded MeadianReadLen=151 20thPercentileLength=145 echo kmer=141 SRR6941586 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,972 [index] number of k-mers: 66,720,672 [index] number of equivalence classes: 111,837 [quant] running in paired-end mode [quant] will process pair 1: SRR6941586-trimmed-pair1.fastq SRR6941586-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 20,078,677 reads, 6,138,052 reads pseudoaligned [quant] estimated average fragment length: 198.228 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 903 rounds 52973 SRR6941586.ke.tsv 35125 SRR6941586.se.tsv 88098 total ==> SRR6941586.ke.tsv <== target_id length eff_length est_counts tpm PNS24245 936 739.178 0 0 PNS24247 1044 846.772 2.72343 0.277951 PNS24249 1928 1730.77 18.8297 0.940207 PNS24246 1044 846.772 2.72343 0.277951 PNS24248 1044 846.772 2.72343 0.277951 PNS24244 1471 1273.77 0 0 PNS24243 293 120.828 0 0 KQK14069 1603 1405.77 560.101 34.4327 KQK14071 474 283.077 11.3661 3.46998 ==> SRR6941586.se.tsv <== BRADI_1g14170v3 679 BRADI_1g53295v3 2 BRADI_1g59795v3 8 BRADI_1g07683v3 0 BRADI_1g00485v3 0 BRADI_1g20270v3 43 BRADI_1g74790v3 8 BRADI_1g09890v3 0 BRADI_1g77505v3 6 BRADI_1g48960v3 0 SRR6941586 completed mapping pipeline successfully