Starting /dee2/code/volunteer_pipeline.sh SRR6941590 current disk space = 1551304097792 free memory = 1367603516 SRR6941590 SRAfilesize fb1ce848947acb7ef551f6c8683704a4 SRR6941590.sra SRR6941590.sra file validated SRR6941590 is paired end SRR6941590 is conventional basespace SRR6941590 read1 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR6941590_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 19.412 18.0 18.0 18.0 18.0 32.0 2 27.8815 28.0 27.0 30.0 25.0 31.0 3 29.851 31.0 29.0 33.0 27.0 33.0 4 31.7225 33.0 31.0 33.0 29.0 33.0 5 32.45025 33.0 33.0 33.0 32.0 34.0 6 36.43575 38.0 36.0 38.0 34.0 38.0 7 37.058 38.0 38.0 38.0 35.0 38.0 8 37.45675 38.0 38.0 38.0 37.0 38.0 9 37.63025 38.0 38.0 38.0 38.0 38.0 10-14 37.5153 38.0 38.0 38.0 37.6 38.0 15-19 37.314949999999996 38.0 38.0 38.0 36.6 38.0 20-24 37.5173 38.0 38.0 38.0 37.6 38.0 25-29 37.58515 38.0 38.0 38.0 37.8 38.0 30-34 37.630649999999996 38.0 38.0 38.0 38.0 38.0 35-39 37.554100000000005 38.0 38.0 38.0 38.0 38.0 40-44 37.51090000000001 38.0 38.0 38.0 37.8 38.0 45-49 37.47905 38.0 38.0 38.0 37.6 38.0 50-54 37.34250000000001 38.0 38.0 38.0 36.8 38.0 55-59 37.19375 38.0 38.0 38.0 36.6 38.0 60-64 37.310900000000004 38.0 38.0 38.0 37.0 38.0 65-69 37.268449999999994 38.0 38.0 38.0 36.6 38.0 70-74 37.2782 38.0 38.0 38.0 36.6 38.0 75-79 37.24145 38.0 38.0 38.0 36.6 38.0 80-84 37.2943 38.0 38.0 38.0 37.0 38.0 85-89 37.1876 38.0 38.0 38.0 36.2 38.0 90-94 36.05195 38.0 36.8 38.0 30.2 38.0 95-99 36.6996 38.0 37.8 38.0 34.6 38.0 100-104 37.0724 38.0 38.0 38.0 35.8 38.0 105-109 36.945350000000005 38.0 38.0 38.0 35.4 38.0 110-114 36.90955 38.0 38.0 38.0 35.2 38.0 115-119 36.701800000000006 38.0 38.0 38.0 34.8 38.0 120-124 36.53985 38.0 38.0 38.0 34.4 38.0 125-129 36.411500000000004 38.0 38.0 38.0 34.0 38.0 130-134 36.5065 38.0 38.0 38.0 34.4 38.0 135-139 36.128550000000004 38.0 37.6 38.0 33.2 38.0 140-144 35.916900000000005 38.0 37.4 38.0 33.0 38.0 145-149 35.45935 38.0 36.2 38.0 31.8 38.0 150-151 32.705375 37.0 33.5 38.0 16.5 38.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 5 1.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 2.0 13 0.0 14 0.0 15 0.0 16 1.0 17 1.0 18 0.0 19 2.0 20 1.0 21 2.0 22 1.0 23 7.0 24 5.0 25 4.0 26 5.0 27 14.0 28 16.0 29 26.0 30 31.0 31 29.0 32 54.0 33 77.0 34 141.0 35 236.0 36 572.0 37 2772.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 39.75054229934924 18.031453362255963 7.483731019522777 34.73427331887201 2 22.705676419104776 15.453863465866466 33.758439609902474 28.08202050512628 3 20.075000000000003 22.15 26.75 31.025000000000002 4 25.224999999999998 30.3 22.45 22.025 5 23.5 35.275 23.0 18.224999999999998 6 20.025000000000002 36.35 23.150000000000002 20.474999999999998 7 15.375 26.700000000000003 40.300000000000004 17.625 8 17.4 23.525 30.075000000000003 28.999999999999996 9 18.25 23.25 32.475 26.025 10-14 21.705 30.240000000000002 23.9 24.154999999999998 15-19 21.490000000000002 28.26 26.135 24.115000000000002 20-24 20.315 29.360000000000003 26.185000000000002 24.14 25-29 22.6 28.455000000000002 25.490000000000002 23.455000000000002 30-34 22.105 29.32 24.29 24.285 35-39 21.665 28.615000000000002 25.955000000000002 23.765 40-44 21.14 28.04 25.990000000000002 24.83 45-49 21.115000000000002 28.110000000000003 26.745 24.03 50-54 21.795 28.02 25.629999999999995 24.555 55-59 21.224999999999998 28.305000000000003 25.525 24.945 60-64 20.380000000000003 27.889999999999997 26.455000000000002 25.275 65-69 21.490000000000002 28.384999999999998 25.09 25.035 70-74 22.900000000000002 27.675 24.435000000000002 24.990000000000002 75-79 21.64 28.015 25.919999999999998 24.425 80-84 23.015 28.305000000000003 24.845 23.835 85-89 22.575 26.955000000000002 25.979999999999997 24.490000000000002 90-94 21.18 28.33 25.77 24.72 95-99 21.6 28.994999999999997 24.445 24.959999999999997 100-104 21.775 28.599999999999998 24.635 24.990000000000002 105-109 21.63 28.305000000000003 25.624999999999996 24.44 110-114 21.735 28.22 25.4 24.645 115-119 21.345 28.749999999999996 25.005 24.9 120-124 20.905 28.875 22.66 27.560000000000002 125-129 22.105 28.83 24.305 24.759999999999998 130-134 21.695 29.38 23.47 25.455 135-139 22.785 29.160000000000004 23.835 24.22 140-144 22.775000000000002 28.754999999999995 23.46 25.009999999999998 145-149 21.985 28.105000000000004 24.365000000000002 25.545 150-151 20.8125 29.575000000000003 23.175 26.437500000000004 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.5 11 0.5 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 1.0 18 1.0 19 0.0 20 0.5 21 1.5 22 3.5 23 2.5 24 1.0 25 3.0 26 6.0 27 9.5 28 9.5 29 12.0 30 21.5 31 25.5 32 30.5 33 30.5 34 27.0 35 39.5 36 88.0 37 188.0 38 203.5 39 162.0 40 195.5 41 225.5 42 212.0 43 211.0 44 191.0 45 174.5 46 160.0 47 127.0 48 122.5 49 101.5 50 89.5 51 88.0 52 91.0 53 109.0 54 128.5 55 155.5 56 141.5 57 101.5 58 94.0 59 91.5 60 70.5 61 45.5 62 32.0 63 30.0 64 30.0 65 27.0 66 18.0 67 10.0 68 8.0 69 5.0 70 6.5 71 10.5 72 9.0 73 5.5 74 2.5 75 2.5 76 3.5 77 3.0 78 2.0 79 0.5 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content warn #Base N-Count 1 7.8 2 0.025 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 72.625 #Duplication Level Percentage of deduplicated Percentage of total 1 84.85370051635111 61.625 2 8.089500860585199 11.75 3 3.0292598967297764 6.6000000000000005 4 1.549053356282272 4.5 5 0.7228915662650602 2.625 6 0.5163511187607573 2.25 7 0.34423407917383825 1.7500000000000002 8 0.17211703958691912 1.0 9 0.17211703958691912 1.125 >10 0.5163511187607573 5.45 >50 0.034423407917383825 1.325 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCATTAAT 53 1.325 No Hit GGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTACGTT 23 0.575 No Hit GTTGAAACTAGCATATTGGAAGATTAATCGGCCAAAATAACCATGAGCAG 20 0.5 No Hit GCCAAAATAACCATGAGCAGCCACAATATTATAAGTCTCTTCCTCTTGAC 18 0.44999999999999996 No Hit GCGGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTACG 17 0.42500000000000004 No Hit CTCATAAGGACCACCATTGTATAACCATTCATCAACGGATGCAGCTTCCC 17 0.42500000000000004 No Hit GCTTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCA 16 0.4 No Hit GCTGCGATGAAGGCGATAATAAATACAGAAGTTGCGGTCAATAAGGTAGG 15 0.375 No Hit CTTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCAT 15 0.375 No Hit GCCGAAAACACCAGCTACACCTAACATGTGAAATGGATGCATAAGGATGT 12 0.3 No Hit GCTAGTTATCCAGTTGCAGAAGCGACCCCACAGGCTTGTACTTTCGCGTC 12 0.3 No Hit GGAGGGGCTGCGATGAAGGCGATAATAAATACAGAAGTTGCGGTCAATAA 11 0.27499999999999997 No Hit CGAGAGTTGTTGAAACTAGCATATTGGAAGATTAATCGGCCAAAATAACC 11 0.27499999999999997 No Hit GCATAAGGATGTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAAAGTA 11 0.27499999999999997 No Hit GGAAAAACCTTATCCATTAATAGCGGGAACTTCAAGAGCAGCTAGATCTA 10 0.25 No Hit GTGCTCTGCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTCCTA 10 0.25 No Hit GTCGGTTTCGGGTACAGGTACCCTTTTGTTGAAGGTCGTTCGAGCTTTTC 9 0.22499999999999998 No Hit GGATGCATAAGGATGTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAA 9 0.22499999999999998 No Hit CGGAAAAACCTTATCCATTAATAGCGGGAACTTCAAGAGCAGCTAGATCT 9 0.22499999999999998 No Hit GTACAAGGCCCGGGAACGGATTCACCGCCGTATGGCTGACCGGCGATTAC 9 0.22499999999999998 No Hit CTCCACGCGGCATTGCTCCGTCAGGCTTTCGCCCATTGCGGAAAATTCCC 9 0.22499999999999998 No Hit AGAGAAGCTTCCTTGACCAATAGGGTAAATCAAGAAAACAGCAGTCGCAG 8 0.2 No Hit CGCAGAAGTAGGAATAATAGCACCAGAGATAATATTGTTTCCATAAAGTA 8 0.2 No Hit GGCCAAAATAACCATGAGCAGCCACAATATTATAAGTCTCTTCCTCTTGA 8 0.2 No Hit TTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCATT 8 0.2 No Hit GCGGAAAAACCTTATCCATTAATAGCGGGAACTTCAAGAGCAGCTAGATC 8 0.2 No Hit GTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTC 7 0.17500000000000002 No Hit GTACAAAGGGCAGGGACGTAGTCAACGCGAGCTGATGACTCGCGCTTACT 7 0.17500000000000002 No Hit TTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCATTAATA 7 0.17500000000000002 No Hit GTTCGCTGGAGTAGGCTGCGAGGCCCAAGCGGCCAAGGAGTGCAGCGGCC 7 0.17500000000000002 No Hit CTCACGACCCATATAACAAGCTACACCAAGTAAGAAGTGTAGAACAATTA 7 0.17500000000000002 No Hit GTGCTAGTTATCCAGTTGCAGAAGCGACCCCACAGGCTTGTACTTTCGCG 7 0.17500000000000002 No Hit GGCAATTTCAAGCACTCTTTGACTCTCTTTTCAAAGTCCTTTTCATCTTT 7 0.17500000000000002 No Hit GGAATAATAGCACCAGAGATAATATTGTTTCCATAAAGTAAAGAACCAGA 7 0.17500000000000002 No Hit CACCATTGTATAACCATTCATCAACGGATGCAGCTTCCCAAATTGGGTAA 7 0.17500000000000002 No Hit CTCGCGAATACCATCAATATCTACTGGAGGGGCTGCGATGAAGGCGATAA 7 0.17500000000000002 No Hit CGGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTACGT 6 0.15 No Hit AAAAATTCTTATATGTTAGCGGAAAAACCTTATCCATTAATAGCGGGAAC 6 0.15 No Hit CCCATATAACAAGCTACACCAAGTAAGAAGTGTAGAACAATTAGCTCATA 6 0.15 No Hit ATCCGATTGCCGCAGAAGTAGGAATAATAGCACCAGAGATAATATTGTTT 6 0.15 No Hit CCCAAGTATTAATAACGCGACCTTGACTATCAACTACAGATTGGTTGAAA 6 0.15 No Hit AGGAATAATAGCACCAGAGATAATATTGTTTCCATAAAGTAAAGAACCAG 6 0.15 No Hit CCCTCATTAGCAGATTCATTTTCAGTAGTTTCCCTGATCAAACTAGAGGT 6 0.15 No Hit CATATAACAAGCTACACCAAGTAAGAAGTGTAGAACAATTAGCTCATAAG 6 0.15 No Hit CAGAGATAATATTGTTTCCATAAAGTAAAGAACCAGAAACAGGCTCGCGA 6 0.15 No Hit GTGAAATGGATGCATAAGGATGTTGTGCTCTGCCTGGAATACAATCATAA 6 0.15 No Hit GGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTACGTTC 6 0.15 No Hit ATAACAAGCTACACCAAGTAAGAAGTGTAGAACAATTAGCTCATAAGGAC 6 0.15 No Hit ACCACATGCTCCACCGCTTGTGCGGGCCCCCGTCAATTCCTTTGAGTTTC 6 0.15 No Hit GGGGCTGCGATGAAGGCGATAATAAATACAGAAGTTGCGGTCAATAAGGT 6 0.15 No Hit CCAGATATTCCTAAAGGCATACCATCAGAGAAGCTTCCTTGACCAATAGG 6 0.15 No Hit GCACGGTTGATGATATCAGCCCAAGTATTAATAACGCGACCTTGACTATC 5 0.125 No Hit CGCGAATACCATCAATATCTACTGGAGGGGCTGCGATGAAGGCGATAATA 5 0.125 No Hit GTGCAATCCGATTGCCGCAGAAGTAGGAATAATAGCACCAGAGATAATAT 5 0.125 No Hit ACCAGCTACACCTAACATGTGAAATGGATGCATAAGGATGTTGTGCTCTG 5 0.125 No Hit GCGATAATAAATACAGAAGTTGCGGTCAATAAGGTAGGGATCATCAAAAC 5 0.125 No Hit AGATAATATTGTTTCCATAAAGTAAAGAACCAGAAACAGGCTCGCGAATA 5 0.125 No Hit CAGAGAAGCTTCCTTGACCAATAGGGTAAATCAAGAAAACAGCAGTCGCA 5 0.125 No Hit GGTTGATGATATCAGCCCAAGTATTAATAACGCGACCTTGACTATCAACT 5 0.125 No Hit GCTGAATATGCAACAGCAATCCAAGGGCGCATACCCAAACGGAAACTAAG 5 0.125 No Hit CAATATTATAAGTCTCTTCCTCTTGACCAAATTTGTAACCCTCATTAGCA 5 0.125 No Hit ATCAGCCCAAGTATTAATAACGCGACCTTGACTATCAACTACAGATTGGT 5 0.125 No Hit TTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCATTA 5 0.125 No Hit CCAGATTCCTACTACAGGCCAAGCAGCCAAGAAGAAGTGTAAAGAACGAG 5 0.125 No Hit CCGAAAACACCAGCTACACCTAACATGTGAAATGGATGCATAAGGATGTT 5 0.125 No Hit GTGTGTACAAGGCCCGGGAACGGATTCACCGCCGTATGGCTGACCGGCGA 5 0.125 No Hit CATCAGAGAAGCTTCCTTGACCAATAGGGTAAATCAAGAAAACAGCAGTC 5 0.125 No Hit AGCGGAAAAACCTTATCCATTAATAGCGGGAACTTCAAGAGCAGCTAGAT 5 0.125 No Hit GCTTCCTTGACCAATAGGGTAAATCAAGAAAACAGCAGTCGCAGCTGCAA 5 0.125 No Hit GATGCATAAGGATGTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAAA 5 0.125 No Hit GTCAATAAGGTAGGGATCATCAAAACACCGAACCATCCGATGTAAAGACG 5 0.125 No Hit CTTTCTTTTCCTCTGGCTACTAAGATGTTTCAGTTCGCCAGGTTGTCTCT 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.025 0.0 0.0 0.0 0.0 50-51 0.025 0.0 0.0 0.0 0.0 52-53 0.025 0.0 0.0 0.0 0.0 54-55 0.025 0.0 0.0 0.0 0.0 56-57 0.025 0.0 0.0 0.0 0.0 58-59 0.025 0.0 0.0 0.0 0.0 60-61 0.037500000000000006 0.0 0.0 0.0 0.0 62-63 0.05 0.0 0.0 0.0 0.0 64-65 0.0625 0.0 0.0 0.0 0.0 66-67 0.075 0.0 0.0 0.0 0.0 68-69 0.1 0.0 0.0 0.0 0.0 70-71 0.1125 0.0 0.0 0.0 0.0 72-73 0.125 0.0 0.0 0.0 0.0 74-75 0.125 0.0 0.0 0.0 0.0 76-77 0.16249999999999998 0.0 0.0 0.0 0.0 78-79 0.2 0.0 0.0 0.0 0.0 80-81 0.25 0.0 0.0 0.0 0.0 82-83 0.375 0.0 0.0 0.0 0.0 84-85 0.475 0.0 0.0 0.0 0.0 86-87 0.5125 0.0 0.0 0.0 0.0 88-89 0.575 0.0 0.0 0.0 0.0 90-91 0.6875 0.0 0.0 0.0 0.0 92-93 0.8125 0.0 0.0 0.0 0.0 94-95 1.05 0.0 0.0 0.0 0.0 96-97 1.275 0.0 0.0 0.0 0.0 98-99 1.6375 0.0 0.0 0.0 0.0 100-101 1.925 0.0 0.0 0.0 0.0 102-103 2.275 0.0 0.0 0.0 0.0 104-105 2.9375 0.0 0.0 0.0 0.0 106-107 3.3375000000000004 0.0 0.0 0.0 0.0 108-109 3.7375 0.0 0.0 0.0 0.0 110-111 4.1 0.0 0.0 0.0 0.0 112-113 4.6625 0.0 0.0 0.0 0.0 114-115 5.1875 0.0 0.0 0.0 0.0 116-117 5.824999999999999 0.0 0.0 0.0 0.0 118-119 6.525 0.0 0.0 0.0 0.0 120-121 7.1625 0.0 0.0 0.0 0.0 122-123 7.8375 0.0 0.0 0.0 0.0 124-125 8.337499999999999 0.0 0.0 0.0 0.0 126-127 9.225000000000001 0.0 0.0 0.0 0.0 128-129 9.9375 0.0 0.0 0.0 0.0 130-131 10.912500000000001 0.0 0.0 0.0 0.0 132-133 11.5125 0.0 0.0 0.0 0.0 134-135 12.3375 0.0 0.0 0.0 0.0 136-137 13.3625 0.0 0.0 0.0 0.0 138-139 14.0625 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TGATTGC 10 0.0068396386 144.9375 4 GTGATTG 10 0.0068396386 144.9375 3 AAAAAAA 35 0.0035454615 20.705357 60-64 >>END_MODULE SRR6941590 read2 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR6941590_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 33.06175 33.0 33.0 34.0 32.0 34.0 2 33.1475 34.0 33.0 34.0 33.0 34.0 3 33.1675 34.0 33.0 34.0 33.0 34.0 4 33.14925 34.0 33.0 34.0 33.0 34.0 5 33.07525 34.0 33.0 34.0 33.0 34.0 6 37.2975 38.0 38.0 38.0 37.0 38.0 7 37.284 38.0 38.0 38.0 37.0 38.0 8 37.282 38.0 38.0 38.0 37.0 38.0 9 37.212 38.0 38.0 38.0 37.0 38.0 10-14 36.970749999999995 38.0 38.0 38.0 36.2 38.0 15-19 37.1017 38.0 38.0 38.0 36.6 38.0 20-24 37.245999999999995 38.0 38.0 38.0 37.0 38.0 25-29 37.21265 38.0 38.0 38.0 37.0 38.0 30-34 36.77505 38.0 38.0 38.0 35.4 38.0 35-39 36.6114 38.0 38.0 38.0 34.8 38.0 40-44 37.108900000000006 38.0 38.0 38.0 36.6 38.0 45-49 37.18205 38.0 38.0 38.0 37.0 38.0 50-54 37.105850000000004 38.0 38.0 38.0 37.0 38.0 55-59 37.050149999999995 38.0 38.0 38.0 36.4 38.0 60-64 37.15860000000001 38.0 38.0 38.0 36.8 38.0 65-69 36.99395 38.0 38.0 38.0 36.2 38.0 70-74 36.7154 38.0 38.0 38.0 35.4 38.0 75-79 36.9222 38.0 38.0 38.0 36.0 38.0 80-84 36.675599999999996 38.0 38.0 38.0 35.2 38.0 85-89 36.37134999999999 38.0 37.8 38.0 33.8 38.0 90-94 36.7541 38.0 38.0 38.0 35.4 38.0 95-99 36.58375 38.0 38.0 38.0 34.6 38.0 100-104 35.682249999999996 38.0 37.0 38.0 30.4 38.0 105-109 36.0229 38.0 37.2 38.0 32.2 38.0 110-114 36.3854 38.0 38.0 38.0 34.0 38.0 115-119 36.32875 38.0 38.0 38.0 34.0 38.0 120-124 35.9429 38.0 37.6 38.0 32.4 38.0 125-129 35.6166 38.0 36.4 38.0 31.8 38.0 130-134 35.711749999999995 38.0 36.0 38.0 32.4 38.0 135-139 31.7763 36.6 26.6 38.0 20.6 38.0 140-144 33.427749999999996 37.0 31.2 38.0 25.0 38.0 145-149 34.366550000000004 38.0 35.0 38.0 27.4 38.0 150-151 29.858249999999998 35.5 27.5 38.0 13.5 38.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 8.0 3 2.0 4 4.0 5 2.0 6 4.0 7 0.0 8 1.0 9 1.0 10 2.0 11 0.0 12 1.0 13 1.0 14 1.0 15 0.0 16 2.0 17 2.0 18 1.0 19 2.0 20 1.0 21 2.0 22 5.0 23 3.0 24 5.0 25 10.0 26 23.0 27 17.0 28 24.0 29 31.0 30 43.0 31 56.0 32 74.0 33 106.0 34 176.0 35 292.0 36 825.0 37 2273.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.974999999999994 18.9 12.55 24.575 2 28.37837837837838 19.06906906906907 33.80880880880881 18.743743743743742 3 21.799047857679778 22.199949887246305 35.83061889250814 20.170383362565772 4 25.31296945418127 30.67100650976465 23.935903855783675 20.080120180270406 5 26.61492238357536 33.700550826239365 22.684026039058587 17.00050075112669 6 22.85 33.900000000000006 24.05 19.2 7 19.825 20.025000000000002 40.725 19.425 8 22.25 22.05 29.4 26.3 9 23.599999999999998 21.85 31.4 23.150000000000002 10-14 26.08 24.45 28.215 21.255 15-19 24.775 24.68 28.92 21.625 20-24 26.245 24.245 29.24 20.27 25-29 25.837583758375835 24.63746374637464 28.292829282928295 21.232123212321234 30-34 25.842584258425845 24.627462746274627 28.587858785878588 20.94209420942094 35-39 25.490000000000002 25.805 27.689999999999998 21.015 40-44 24.855 25.785000000000004 28.139999999999997 21.22 45-49 25.006250312515625 26.3913195659783 27.50637531876594 21.096054802740134 50-54 25.016250812540626 25.676283814190707 27.986399319965997 21.321066053302665 55-59 24.635 25.900000000000002 27.665 21.8 60-64 25.36626831341567 24.466223311165557 28.546427321366068 21.621081054052702 65-69 25.713857078561787 24.863729559433914 27.77916687503125 21.643246486973048 70-74 25.271317829457363 25.096274068517125 28.24206051512878 21.390347586896723 75-79 25.480000000000004 25.130000000000003 28.345 21.044999999999998 80-84 25.966298314915747 24.88124406220311 28.531426571328566 20.621031051552578 85-89 25.615 25.080000000000002 27.18 22.125 90-94 25.22 24.975 27.405 22.400000000000002 95-99 24.915000000000003 25.069999999999997 28.38 21.634999999999998 100-104 25.52 25.124999999999996 28.26 21.095 105-109 26.965 24.395 28.055000000000003 20.585 110-114 25.661283064153206 25.071253562678137 27.731386569328464 21.536076803840192 115-119 26.116305815290765 25.661283064153206 27.181359067953398 21.04105205260263 120-124 25.47127356367818 26.32131606580329 26.341317065853293 21.86609330466523 125-129 25.69 26.35 27.11 20.849999999999998 130-134 25.435000000000002 26.229999999999997 26.87 21.465 135-139 25.845000000000002 26.229999999999997 27.005000000000003 20.919999999999998 140-144 27.105 25.39 27.305 20.200000000000003 145-149 27.02 25.629999999999995 27.779999999999998 19.57 150-151 26.8375 25.05 28.3375 19.775000000000002 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.5 4 0.5 5 0.0 6 0.0 7 0.0 8 0.0 9 0.5 10 0.5 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.5 19 0.5 20 1.5 21 2.5 22 1.5 23 3.5 24 5.5 25 6.0 26 7.5 27 9.0 28 13.0 29 18.5 30 20.5 31 21.5 32 22.0 33 32.5 34 51.0 35 61.0 36 88.0 37 123.0 38 154.5 39 176.5 40 185.0 41 180.5 42 167.5 43 195.0 44 218.5 45 185.0 46 165.0 47 150.0 48 131.5 49 116.0 50 87.0 51 85.5 52 91.5 53 97.5 54 122.5 55 149.0 56 138.0 57 111.0 58 97.5 59 89.5 60 77.0 61 67.0 62 66.5 63 48.0 64 24.0 65 14.5 66 15.0 67 21.5 68 18.0 69 8.5 70 6.0 71 6.5 72 8.5 73 7.5 74 7.0 75 7.0 76 5.5 77 4.0 78 2.0 79 1.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.1 3 0.22499999999999998 4 0.15 5 0.15 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.01 30-34 0.01 35-39 0.0 40-44 0.0 45-49 0.005 50-54 0.005 55-59 0.0 60-64 0.005 65-69 0.015 70-74 0.025 75-79 0.0 80-84 0.005 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.005 115-119 0.005 120-124 0.005 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 70.35 #Duplication Level Percentage of deduplicated Percentage of total 1 84.11513859275054 59.175 2 7.782515991471215 10.95 3 3.482587064676617 7.35 4 1.6702203269367448 4.7 5 0.8173418621179815 2.875 6 0.46197583511016344 1.95 7 0.35536602700781805 1.7500000000000002 8 0.14214641080312723 0.8 9 0.24875621890547264 1.575 >10 0.9239516702203269 8.875 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source ATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAA 26 0.65 No Hit GGCTTGCGGTGGATACCTAGGCACCCAGAGACGAGGAAGGGCGTAGCAAG 22 0.5499999999999999 No Hit GTTCGATCCTGGCTCAGGATGAACGCTGGCGGCATGCTTAACACATGCAA 21 0.525 No Hit GTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGATCTT 18 0.44999999999999996 No Hit GCTTGTTATATGGGTCGTGAGTGGGAACTTAGTTTCCGTTTGGGTATGCG 18 0.44999999999999996 No Hit GTCAAGGTCGCGTTATTAATACTTGGGCTGATATCATCAACCGTGCTAAT 16 0.4 No Hit ATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCA 15 0.375 No Hit GCTGCGACTGCTGTTTTCTTGATTTACCCTATTGGTCAAGGAAGCTTCTC 15 0.375 No Hit TATCTATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTT 14 0.35000000000000003 No Hit GCTAATTGTTCTACACTTCTTACTTGGTGTAGCTTGTTATATGGGTCGTG 13 0.325 No Hit GGGAACTTAGTTTCCGTTTGGGTATGCGCCCTTGGATTGCTGTTGCATAT 13 0.325 No Hit GTCGTGAGTGGGAACTTAGTTTCCGTTTGGGTATGCGCCCTTGGATTGCT 12 0.3 No Hit TATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAA 12 0.3 No Hit GTGGGAACTTAGTTTCCGTTTGGGTATGCGCCCTTGGATTGCTGTTGCAT 12 0.3 No Hit GTTCAACCTAAATGGATTCAATTTCAACCAATCTGTAGTTGATAGTCAAG 12 0.3 No Hit AGCACAACATCCTTATGCATCCATTTCACATGTTAGGTGTAGCTGGTGTT 11 0.27499999999999997 No Hit GGTCGCTTCTGCAACTGGATAACTAGCACTGAAAATCGTCTTTACATCGG 11 0.27499999999999997 No Hit AGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGATCT 11 0.27499999999999997 No Hit CCTTACCAGGGCTTGACATGCCGCGAATCCTCTTGAAAGAGAGGGGTGCC 11 0.27499999999999997 No Hit GTTTGATCAGGGAAACTACTGAAAATGAATCTGCTAATGAGGGTTACAAA 11 0.27499999999999997 No Hit GTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGATCTTACCATGAC 11 0.27499999999999997 No Hit ATTTGGTCAAGAGGAAGAGACTTATAATATTGTGGCTGCTCATGGTTATT 10 0.25 No Hit GGCGGGACTACCCGCTGAGTTTAAGCATATAAATAAGCGGAGGAGAAGAA 10 0.25 No Hit GTTTTGATGATCCCTACCTTATTGACCGCAACTTCTGTATTTATTATCGC 10 0.25 No Hit ATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCTAAATGG 10 0.25 No Hit CATCAACCGTGCTAATCTTGGTATGGAAGTAATGCACGAACGTAATGCTC 10 0.25 No Hit CTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGAT 9 0.22499999999999998 No Hit GGAAACTACTGAAAATGAATCTGCTAATGAGGGTTACAAATTTGGTCAAG 9 0.22499999999999998 No Hit GTCTTTACATCGGATGGTTCGGTGTTTTGATGATCCCTACCTTATTGACC 9 0.22499999999999998 No Hit CGCGAAAGTACAAGCCTGTGGGGTCGCTTCTGCAACTGGATAACTAGCAC 9 0.22499999999999998 No Hit GTTAGGTGTAGCTGGTGTTTTCGGCGGTTCCCTATTCAGTGCTATGCATG 9 0.22499999999999998 No Hit GCACAACATCCTTATGCATCCATTTCACATGTTAGGTGTAGCTGGTGTTT 9 0.22499999999999998 No Hit ATTATCTATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAAT 9 0.22499999999999998 No Hit TTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAA 8 0.2 No Hit CCTAAATGGATTCAATTTCAACCAATCTGTAGTTGATAGTCAAGGTCGCG 8 0.2 No Hit ATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATA 8 0.2 No Hit GTTTCAACAACTCTCGTTCTTTACACTTCTTCTTGGCTGCTTGGCCTGTA 8 0.2 No Hit GTTTGGGTATGCGCCCTTGGATTGCTGTTGCATATTCAGCTCCTGTTGCA 7 0.17500000000000002 No Hit CTGGTACTTTCAACTTTATGATTGTATTCCAGGCAGAGCACAACATCCTT 7 0.17500000000000002 No Hit TTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAG 7 0.17500000000000002 No Hit CCTGCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGCAA 7 0.17500000000000002 No Hit GGAAGCTTCTCTGATGGTATGCCTTTAGGAATATCTGGTACTTTCAACTT 7 0.17500000000000002 No Hit GCCTGTAGTAGGAATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGT 7 0.17500000000000002 No Hit ATTCAGTGCTATGCATGGTTCCTTGGTAACCTCTAGTTTGATCAGGGAAA 7 0.17500000000000002 No Hit GGTACTTTCAACTTTATGATTGTATTCCAGGCAGAGCACAACATCCTTAT 7 0.17500000000000002 No Hit GTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGATCTTACCATGACTG 7 0.17500000000000002 No Hit CAACATCCTTATGCATCCATTTCACATGTTAGGTGTAGCTGGTGTTTTCG 7 0.17500000000000002 No Hit CAAGATCTTACCATGACTGCAATTTTAGAGAGACGCGAAAGTACAAGCCT 6 0.15 No Hit GGGAAACTACTGAAAATGAATCTGCTAATGAGGGTTACAAATTTGGTCAA 6 0.15 No Hit TGGGAACTTAGTTTCCGTTTGGGTATGCGCCCTTGGATTGCTGTTGCATA 6 0.15 No Hit TGAAAATGAATCTGCTAATGAGGGTTACAAATTTGGTCAAGAGGAAGAGA 6 0.15 No Hit TGAAAATCGTCTTTACATCGGATGGTTCGGTGTTTTGATGATCCCTACCT 6 0.15 No Hit CTATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAA 6 0.15 No Hit AAACAATATTATCTCTGGTGCTATTATTCCTACTTCTGCGGCAATCGGAT 6 0.15 No Hit GGATGGTTCGGTGTTTTGATGATCCCTACCTTATTGACCGCAACTTCTGT 6 0.15 No Hit GACCAGGCCCGGGCGACTGTTTACCAAAAACACAGGTCTCCGCAAAGTCG 6 0.15 No Hit TCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGA 6 0.15 No Hit TGTAGCTGGTGTTTTCGGCGGTTCCCTATTCAGTGCTATGCATGGTTCCT 6 0.15 No Hit GCTAACTCCAAAAACCCGTCCTCAGTTCGGATTGCAGGCTGCAACTCGCC 6 0.15 No Hit TGATAGTCAAGGTCGCGTTATTAATACTTGGGCTGATATCATCAACCGTG 6 0.15 No Hit GCTGGCGGCATGCTTAACACATGCAAGTCGAACGGGAAGTGGTGTTTCCA 5 0.125 No Hit CCGCAACTTCTGTATTTATTATCGCCTTCATCGCAGCCCCTCCAGTAGAT 5 0.125 No Hit GGTAACCTCTAGTTTGATCAGGGAAACTACTGAAAATGAATCTGCTAATG 5 0.125 No Hit ATCGGATTGCACTTTTACCCAATTTGGGAAGCTGCATCCGTTGATGAATG 5 0.125 No Hit CCTGGCTCAGGATGAACGCTGGCGGCATGCTTAACACATGCAAGTCGAAC 5 0.125 No Hit TAGGAATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCTA 5 0.125 No Hit GCAATTTTAGAGAGACGCGAAAGTACAAGCCTGTGGGGTCGCTTCTGCAA 5 0.125 No Hit GAGACTTATAATATTGTGGCTGCTCATGGTTATTTTGGCCGATTAATCTT 5 0.125 No Hit GCTAGTTTCAACAACTCTCGTTCTTTACACTTCTTCTTGGCTGCTTGGCC 5 0.125 No Hit CATGACTGCAATTTTAGAGAGACGCGAAAGTACAAGCCTGTGGGGTCGCT 5 0.125 No Hit GCTTGCGGTGGATACCTAGGCACCCAGAGACGAGGAAGGGCGTAGCAAGC 5 0.125 No Hit ATGCGCCCTTGGATTGCTGTTGCATATTCAGCTCCTGTTGCAGCTGCGAC 5 0.125 No Hit AGTCAAGGTCGCGTTATTAATACTTGGGCTGATATCATCAACCGTGCTAA 5 0.125 No Hit GTTGCATATTCAGCTCCTGTTGCAGCTGCGACTGCTGTTTTCTTGATTTA 5 0.125 No Hit CTTGATTTACCCTATTGGTCAAGGAAGCTTCTCTGATGGTATGCCTTTAG 5 0.125 No Hit TGATGGTATTCGCGAGCCTGTTTCTGGTTCTTTACTTTATGGAAACAATA 5 0.125 No Hit GGTCAAGGAAGCTTCTCTGATGGTATGCCTTTAGGAATATCTGGTACTTT 5 0.125 No Hit CACATGTTAGGTGTAGCTGGTGTTTTCGGCGGTTCCCTATTCAGTGCTAT 5 0.125 No Hit CCTGAACAGACCGCCGGTGTTAAGCCGGAGGAAGGAGAGGATGAGGCCAA 5 0.125 No Hit GGCCGATTAATCTTCCAATATGCTAGTTTCAACAACTCTCGTTCTTTACA 5 0.125 No Hit GTACAAGCCTGTGGGGTCGCTTCTGCAACTGGATAACTAGCACTGAAAAT 5 0.125 No Hit CCCGAGTGGGGGCTTTAAGGGTGGTGTGGACGTTGCTGTAGAGCTGCGGG 5 0.125 No Hit GCGAAATTCCTTGTCGGGTAAGTTCCGACCCGCACGAAAGGCGTAACGAT 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.025 0.0 0.0 0.0 0.0 50-51 0.025 0.0 0.0 0.0 0.0 52-53 0.025 0.0 0.0 0.0 0.0 54-55 0.025 0.0 0.0 0.0 0.0 56-57 0.025 0.0 0.0 0.0 0.0 58-59 0.025 0.0 0.0 0.0 0.0 60-61 0.037500000000000006 0.0 0.0 0.0 0.0 62-63 0.05 0.0 0.0 0.0 0.0 64-65 0.0625 0.0 0.0 0.0 0.0 66-67 0.075 0.0 0.0 0.0 0.0 68-69 0.1 0.0 0.0 0.0 0.0 70-71 0.1125 0.0 0.0 0.0 0.0 72-73 0.125 0.0 0.0 0.0 0.0 74-75 0.125 0.0 0.0 0.0 0.0 76-77 0.16249999999999998 0.0 0.0 0.0 0.0 78-79 0.2 0.0 0.0 0.0 0.0 80-81 0.25 0.0 0.0 0.0 0.0 82-83 0.375 0.0 0.0 0.0 0.0 84-85 0.4625 0.0 0.0 0.0 0.0 86-87 0.5125 0.0 0.0 0.0 0.0 88-89 0.575 0.0 0.0 0.0 0.0 90-91 0.6875 0.0 0.0 0.0 0.0 92-93 0.8125 0.0 0.0 0.0 0.0 94-95 1.025 0.0 0.0 0.0 0.0 96-97 1.2125 0.0 0.0 0.0 0.0 98-99 1.5375 0.0 0.0 0.0 0.0 100-101 1.775 0.0 0.0 0.0 0.0 102-103 2.075 0.0 0.0 0.0 0.0 104-105 2.7125000000000004 0.0 0.0 0.0 0.0 106-107 3.1125 0.0 0.0 0.0 0.0 108-109 3.525 0.0 0.0 0.0 0.0 110-111 3.95 0.0 0.0 0.0 0.0 112-113 4.475 0.0 0.0 0.0 0.0 114-115 4.95 0.0 0.0 0.0 0.0 116-117 5.574999999999999 0.0 0.0 0.0 0.0 118-119 6.275 0.0 0.0 0.0 0.0 120-121 6.8875 0.0 0.0 0.0 0.0 122-123 7.5625 0.0 0.0 0.0 0.0 124-125 8.1 0.0 0.0 0.0 0.0 126-127 8.95 0.0 0.0 0.0 0.0 128-129 9.5 0.0 0.0 0.0 0.0 130-131 10.2 0.0 0.0 0.0 0.0 132-133 10.7 0.0 0.0 0.0 0.0 134-135 11.4375 0.0 0.0 0.0 0.0 136-137 12.2625 0.0 0.0 0.0 0.0 138-139 12.8625 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content pass >>END_MODULE Read 1155860 spots for SRR6941590.sra Written 1155860 spots for SRR6941590.sra Read 1155860 spots for SRR6941590.sra Written 1155860 spots for SRR6941590.sra Read 1155860 spots for SRR6941590.sra Written 1155860 spots for SRR6941590.sra Read 1155860 spots for SRR6941590.sra Written 1155860 spots for SRR6941590.sra Read 1155860 spots for SRR6941590.sra Written 1155860 spots for SRR6941590.sra Read 1155860 spots for SRR6941590.sra Written 1155860 spots for SRR6941590.sra Read 1155860 spots for SRR6941590.sra Written 1155860 spots for SRR6941590.sra Read 1155860 spots for SRR6941590.sra Written 1155860 spots for SRR6941590.sra Read 1155860 spots for SRR6941590.sra Written 1155860 spots for SRR6941590.sra Read 1155860 spots for SRR6941590.sra Written 1155860 spots for SRR6941590.sra Read 1155860 spots for SRR6941590.sra Written 1155860 spots for SRR6941590.sra Read 1155860 spots for SRR6941590.sra Written 1155860 spots for SRR6941590.sra Read 1155860 spots for SRR6941590.sra Written 1155860 spots for SRR6941590.sra Read 1155860 spots for SRR6941590.sra Written 1155860 spots for SRR6941590.sra Read 1155860 spots for SRR6941590.sra Written 1155860 spots for SRR6941590.sra Read 1155860 spots for SRR6941590.sra Written 1155860 spots for SRR6941590.sra Read 1155860 spots for SRR6941590.sra Written 1155860 spots for SRR6941590.sra Read 1155860 spots for SRR6941590.sra Written 1155860 spots for SRR6941590.sra Read 1155860 spots for SRR6941590.sra Written 1155860 spots for SRR6941590.sra Read 1155860 spots for SRR6941590.sra Written 1155860 spots for SRR6941590.sra SRR ids: ['SRR6941590.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_rec4ahm4 SRR6941590.sra spots: 23117200 blocks: [[1, 1155860], [1155861, 2311720], [2311721, 3467580], [3467581, 4623440], [4623441, 5779300], [5779301, 6935160], [6935161, 8091020], [8091021, 9246880], [9246881, 10402740], [10402741, 11558600], [11558601, 12714460], [12714461, 13870320], [13870321, 15026180], [15026181, 16182040], [16182041, 17337900], [17337901, 18493760], [18493761, 19649620], [19649621, 20805480], [20805481, 21961340], [21961341, 23117200]] SRR6941590 file size 7811960 SRR6941590 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR6941590 SRR6941590_1.fastq SRR6941590_2.fastq Input file: SRR6941590_1.fastq Paired file: SRR6941590_2.fastq trimmed: SRR6941590-trimmed-pair1.fastq, SRR6941590-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Fri Dec 6 12:24:05 2024 >> started Fri Dec 6 12:24:33 2024 >> done (27.931s) 23117200 read pairs processed; of these: 40639 ( 0.18%) short read pairs filtered out after trimming by size control 36558 ( 0.16%) empty read pairs filtered out after trimming by size control 23040003 (99.67%) read pairs available; of these: 9934833 (43.12%) trimmed read pairs available after processing 13105170 (56.88%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 1 0.00% 19 3 0.00% 20 2 0.00% 21 2 0.00% 22 1 0.00% 23 1 0.00% 24 2 0.00% 25 6 0.00% 26 5 0.00% 27 21 0.00% 28 5 0.00% 29 7 0.00% 30 8 0.00% 31 10 0.00% 32 9 0.00% 33 6 0.00% 34 12 0.00% 35 8 0.00% 36 15 0.00% 37 22 0.00% 38 14 0.00% 39 19 0.00% 40 27 0.00% 41 27 0.00% 42 31 0.00% 43 47 0.00% 44 38 0.00% 45 41 0.00% 46 47 0.00% 47 48 0.00% 48 73 0.00% 49 101 0.00% 50 103 0.00% 51 118 0.00% 52 161 0.00% 53 195 0.00% 54 221 0.00% 55 223 0.00% 56 252 0.00% 57 268 0.00% 58 347 0.00% 59 421 0.00% 60 533 0.00% 61 557 0.00% 62 792 0.00% 63 896 0.00% 64 962 0.00% 65 1119 0.00% 66 1258 0.01% 67 1322 0.01% 68 1605 0.01% 69 1730 0.01% 70 2011 0.01% 71 2430 0.01% 72 2897 0.01% 73 3317 0.01% 74 3648 0.02% 75 4118 0.02% 76 4676 0.02% 77 5284 0.02% 78 5825 0.03% 79 6719 0.03% 80 7643 0.03% 81 8487 0.04% 82 9416 0.04% 83 10554 0.05% 84 13073 0.06% 85 15991 0.07% 86 16552 0.07% 87 18202 0.08% 88 21047 0.09% 89 21525 0.09% 90 22847 0.10% 91 23526 0.10% 92 27476 0.12% 93 27940 0.12% 94 29827 0.13% 95 33837 0.15% 96 33396 0.14% 97 35708 0.15% 98 38956 0.17% 99 40660 0.18% 100 42049 0.18% 101 44200 0.19% 102 46504 0.20% 103 46366 0.20% 104 50306 0.22% 105 52717 0.23% 106 53756 0.23% 107 56815 0.25% 108 59701 0.26% 109 60290 0.26% 110 60983 0.26% 111 65317 0.28% 112 66766 0.29% 113 65874 0.29% 114 74126 0.32% 115 78031 0.34% 116 79704 0.35% 117 79287 0.34% 118 79141 0.34% 119 79017 0.34% 120 80693 0.35% 121 84778 0.37% 122 96034 0.42% 123 94194 0.41% 124 95121 0.41% 125 101610 0.44% 126 93933 0.41% 127 99126 0.43% 128 98777 0.43% 129 104454 0.45% 130 97563 0.42% 131 104776 0.45% 132 110038 0.48% 133 104607 0.45% 134 112411 0.49% 135 108193 0.47% 136 111466 0.48% 137 110353 0.48% 138 122535 0.53% 139 124282 0.54% 140 124630 0.54% 141 145013 0.63% 142 136043 0.59% 143 146084 0.63% 144 154318 0.67% 145 182936 0.79% 146 197462 0.86% 147 234785 1.02% 148 328903 1.43% 149 596267 2.59% 150 3881168 16.85% 151 13105170 56.88% 23040003 reads passed initial QC criterion=sequence-density sequence-density=1.45 sequence-density-rank=1 fanout-score=1.99 fanout-score-rank=30 prefix-density=1.43 prefix-fanout=2.0 sequence=GTATTTAGCCTTG criterion=fanout-score sequence-density=0.03 sequence-density-rank=37 fanout-score=17.41 fanout-score-rank=1 prefix-density=0.22 prefix-fanout=2.1 sequence=AAAAACAGTAGAAGTAGAACAGGTATAAATAAGAAAATCTTAGTTAAGAGGGTTCATGTAAAGAACAGGTTCTAAATCACGATCGATTCCCTTTTCAAAACCTGCTGCAGCAGCTCGGGCTCTTCCTGCATGCCACAAATGGCCCACAAAAAAGAAGAATCCTAGAACAAAATGAGAAGTCGATAACCAACTTCTAGGAGAGACATAATTAACTGCATTGATCTCGGTAGCTACGCCACCCACGGAATTTAAAGAGCCTAAAGGAGCATGGGTCATATATTCCGCTGAACGTCGTTCTTGCCAAGGTTGTATGTCTTTTTTCAACCTACTCAAGTCCAAACCGTTGGGCCCCCTTAGAGGTTCTAACCATGGAGCACGGAGGTCCCAAAAACGCATAGTTTCCCCTCCAAAGATAACCTCTCCCGTTGGGGAACGCATTAGATATTTACCTAAACCTGTGGGTCCTTGAGCAGATCCCACATTAGCTCCAAGACGCTGGTCTCTAACTAGA criterion=sequence-density sequence-density=0.44 sequence-density-rank=1 fanout-score=2.99 fanout-score-rank=28 prefix-density=1.30 prefix-fanout=1.0 sequence=TTGCGTAGTGGATCTGCTGGGGCCTATGCGAAAGCTGGGCCTCACGGATCCTAGAGCGGCAGGCACCGCGTGAGGCTG criterion=fanout-score sequence-density=0.01 sequence-density-rank=38 fanout-score=30.96 fanout-score-rank=1 prefix-density=0.03 prefix-fanout=6.7 sequence=TTTTTTTTTATGAGATTTTTGCTAAAGTTTCATTTACGCCTAATTCACATCGAGTAGACCTTGTTATTGTGAGAATTCTTAATTCAAGAGTTGTAAGGAGGGACTTATGTCACCACAAACAGAAACTAAAGCAAGTGTTGGATTTAAAGCTGGTGTTAAAGATTATAGATTGACTTACTACACCCCGGAGTATGAAACCAAGGATACTGATATCTTGGCAGCATTCCGAGTATCTCCTCAACCTGGGGTTCCGCCCGAAGAAGCAGGGGCTGCAGTAGCTGCCGAATCTTCTACTGGTACATGGACAACTGTTTGGACTGATGGACTTACTAGTCTTGATCGTTACAAAGGACGATGCTATCACATCGAGCCTGTTCCTGGGGAAGACAGTCAATGGATCTGTTATGTAGCTTATCCATTAGATCTATTTGAAGAGGGTTCCGTTACTAACATGTTTACTTCCATTGTAGGTAACGTATTTGGTTTCAAAGCCCTACGTGCTCTACGTCTG SRR6941590 testing PE reads STAR mapping to Ensembl genome Started job on | Dec 06 12:25:36 Started mapping on | Dec 06 12:25:36 Finished on | Dec 06 12:28:01 Mapping speed, Million of reads per hour | 572.03 Number of input reads | 23040003 Average input read length | 290 UNIQUE READS: Uniquely mapped reads number | 13008482 Uniquely mapped reads % | 56.46% Average mapped length | 292.38 Number of splices: Total | 2602686 Number of splices: Annotated (sjdb) | 2354716 Number of splices: GT/AG | 2481852 Number of splices: GC/AG | 31107 Number of splices: AT/AC | 7485 Number of splices: Non-canonical | 82242 Mismatch rate per base, % | 0.31% Deletion rate per base | 0.02% Deletion average length | 2.02 Insertion rate per base | 0.02% Insertion average length | 2.41 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 7325657 % of reads mapped to multiple loci | 31.80% Number of reads mapped to too many loci | 292269 % of reads mapped to too many loci | 1.27% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 4.42% % of reads unmapped: other | 6.05% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 2722025 2722025 2722025 N_multimapping 7325657 7325657 7325657 N_noFeature 5918901 12633089 6085126 N_ambiguous 400602 4769 196014 UnstrandedReadsAssigned:6688979 PositiveStrandReadsAssigned:370624 NegativeStrandReadsAssigned:6727342 Dataset is classified negative stranded MeadianReadLen=151 20thPercentileLength=147 echo kmer=143 SRR6941590 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,972 [index] number of k-mers: 66,720,672 [index] number of equivalence classes: 111,837 [quant] running in paired-end mode [quant] will process pair 1: SRR6941590-trimmed-pair1.fastq SRR6941590-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 23,040,003 reads, 10,523,340 reads pseudoaligned [quant] estimated average fragment length: 212.398 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,107 rounds 52973 SRR6941590.ke.tsv 35125 SRR6941590.se.tsv 88098 total ==> SRR6941590.ke.tsv <== target_id length eff_length est_counts tpm PNS24245 936 725.157 0 0 PNS24247 1044 832.602 11.5551 1.11921 PNS24249 1928 1716.6 22.7023 1.06653 PNS24246 1044 832.602 11.5551 1.11921 PNS24248 1044 832.602 11.5551 1.11921 PNS24244 1471 1259.6 12.6324 0.80877 PNS24243 293 115.726 0 0 KQK14069 1603 1391.6 2633.89 152.636 KQK14071 474 272.246 95.1513 28.1856 ==> SRR6941590.se.tsv <== BRADI_1g14170v3 3287 BRADI_1g53295v3 39 BRADI_1g59795v3 67 BRADI_1g07683v3 0 BRADI_1g00485v3 0 BRADI_1g20270v3 121 BRADI_1g74790v3 5 BRADI_1g09890v3 0 BRADI_1g77505v3 63 BRADI_1g48960v3 0 SRR6941590 completed mapping pipeline successfully