Starting /dee2/code/volunteer_pipeline.sh SRR6941591 current disk space = 1551289225216 free memory = 1602348628 SRR6941591 SRAfilesize 2ee58016419a1c33c481036a1f0267c8 SRR6941591.sra SRR6941591.sra file validated SRR6941591 is paired end SRR6941591 is conventional basespace SRR6941591 read1 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR6941591_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.106 32.0 28.0 33.0 18.0 33.0 2 30.9085 33.0 31.0 33.0 27.0 33.0 3 32.11325 33.0 33.0 33.0 28.0 34.0 4 32.65275 33.0 33.0 34.0 31.0 34.0 5 33.1475 33.0 33.0 34.0 33.0 34.0 6 37.08175 38.0 37.0 38.0 36.0 38.0 7 37.50975 38.0 38.0 38.0 37.0 38.0 8 37.55275 38.0 38.0 38.0 38.0 38.0 9 37.6565 38.0 38.0 38.0 38.0 38.0 10-14 37.5878 38.0 38.0 38.0 37.8 38.0 15-19 37.405899999999995 38.0 38.0 38.0 37.2 38.0 20-24 37.51905000000001 38.0 38.0 38.0 38.0 38.0 25-29 37.6211 38.0 38.0 38.0 38.0 38.0 30-34 37.64755 38.0 38.0 38.0 38.0 38.0 35-39 37.589650000000006 38.0 38.0 38.0 38.0 38.0 40-44 37.549850000000006 38.0 38.0 38.0 38.0 38.0 45-49 37.5264 38.0 38.0 38.0 38.0 38.0 50-54 37.39515 38.0 38.0 38.0 37.4 38.0 55-59 37.247800000000005 38.0 38.0 38.0 37.0 38.0 60-64 37.43425 38.0 38.0 38.0 37.2 38.0 65-69 37.36635 38.0 38.0 38.0 37.2 38.0 70-74 37.360949999999995 38.0 38.0 38.0 37.2 38.0 75-79 37.33135 38.0 38.0 38.0 37.0 38.0 80-84 37.3994 38.0 38.0 38.0 37.0 38.0 85-89 37.32039999999999 38.0 38.0 38.0 36.8 38.0 90-94 36.1705 38.0 36.8 38.0 30.6 38.0 95-99 36.87835 38.0 37.8 38.0 35.4 38.0 100-104 37.22025 38.0 38.0 38.0 36.8 38.0 105-109 37.09735 38.0 38.0 38.0 36.0 38.0 110-114 37.12045 38.0 38.0 38.0 36.0 38.0 115-119 36.9203 38.0 38.0 38.0 35.6 38.0 120-124 36.8057 38.0 38.0 38.0 35.2 38.0 125-129 36.7438 38.0 38.0 38.0 35.0 38.0 130-134 36.7087 38.0 38.0 38.0 35.0 38.0 135-139 36.429500000000004 38.0 38.0 38.0 34.0 38.0 140-144 36.3635 38.0 38.0 38.0 33.8 38.0 145-149 36.001850000000005 38.0 38.0 38.0 33.4 38.0 150-151 33.47875 37.0 34.5 38.0 16.5 38.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 1.0 13 0.0 14 1.0 15 1.0 16 1.0 17 0.0 18 0.0 19 1.0 20 1.0 21 3.0 22 0.0 23 3.0 24 4.0 25 2.0 26 12.0 27 11.0 28 17.0 29 16.0 30 17.0 31 25.0 32 42.0 33 81.0 34 110.0 35 177.0 36 424.0 37 3049.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 40.26044492674987 14.812805208898535 10.037981551817689 34.88876831253391 2 25.68784392196098 17.508754377188595 31.465732866433214 25.337668834417208 3 19.025 26.174999999999997 26.174999999999997 28.625 4 23.375 34.125 22.5 20.0 5 21.25 35.65 24.625 18.475 6 17.175 41.625 22.175 19.025 7 11.600000000000001 30.8 41.625 15.975 8 17.95 28.1 28.9 25.05 9 16.175 25.55 32.625 25.650000000000002 10-14 19.225 34.635 23.665 22.475 15-19 20.62 30.495 26.795 22.09 20-24 19.03 32.535 26.145000000000003 22.29 25-29 21.575 32.09 25.235000000000003 21.099999999999998 30-34 21.099999999999998 33.505 24.18 21.215 35-39 20.369999999999997 31.819999999999997 25.705 22.105 40-44 19.18 31.009999999999998 26.834999999999997 22.975 45-49 19.67 31.619999999999997 27.55 21.16 50-54 21.035 31.395 24.755 22.814999999999998 55-59 20.810000000000002 31.424999999999997 25.324999999999996 22.439999999999998 60-64 19.175 32.12 26.729999999999997 21.975 65-69 20.605 31.819999999999997 24.85 22.725 70-74 21.154999999999998 30.8 24.865000000000002 23.18 75-79 20.919999999999998 31.259999999999998 25.569999999999997 22.25 80-84 22.06 31.05 24.645 22.245 85-89 21.115000000000002 30.115 26.685 22.085 90-94 19.665 32.105 24.69 23.54 95-99 20.419999999999998 31.16 24.8 23.62 100-104 20.075000000000003 31.724999999999998 24.91 23.29 105-109 20.65 30.94 25.56 22.85 110-114 20.115 30.48 25.95 23.455000000000002 115-119 19.725 31.919999999999998 24.345 24.01 120-124 19.685 32.46 22.53 25.324999999999996 125-129 21.060000000000002 31.85 24.385 22.705000000000002 130-134 21.975 33.135 22.259999999999998 22.63 135-139 22.24 31.990000000000002 24.09 21.68 140-144 22.34 31.005 24.7 21.955 145-149 21.18 31.319999999999997 23.07 24.43 150-151 19.900000000000002 33.025 22.75 24.325 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.5 7 0.5 8 0.0 9 0.0 10 0.5 11 1.0 12 0.5 13 0.0 14 0.5 15 1.0 16 0.5 17 0.0 18 0.5 19 0.5 20 0.5 21 1.5 22 3.0 23 4.0 24 4.5 25 5.0 26 9.5 27 11.5 28 11.5 29 18.0 30 22.0 31 23.0 32 32.0 33 40.0 34 44.5 35 52.5 36 129.0 37 269.0 38 278.0 39 254.0 40 305.0 41 324.5 42 256.0 43 211.5 44 229.0 45 230.5 46 191.0 47 141.0 48 135.0 49 105.0 50 76.5 51 58.0 52 45.5 53 47.5 54 51.0 55 57.0 56 48.0 57 30.5 58 31.0 59 36.5 60 34.0 61 26.0 62 15.5 63 10.0 64 12.0 65 25.0 66 23.0 67 6.0 68 4.0 69 4.0 70 1.0 71 1.5 72 2.0 73 1.5 74 0.5 75 1.0 76 1.0 77 1.0 78 1.5 79 0.5 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content warn #Base N-Count 1 7.85 2 0.05 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 64.775 #Duplication Level Percentage of deduplicated Percentage of total 1 85.10227711308374 55.125 2 6.483983018139715 8.4 3 3.049015824006175 5.925 4 1.1578541103820919 3.0 5 0.7333076032419915 2.375 6 0.6561173292165187 2.55 7 0.6947124662292551 3.15 8 0.5017367811655732 2.6 9 0.19297568506368198 1.125 >10 1.3894249324585102 13.900000000000002 >50 0.0385951370127364 1.8499999999999999 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCATTAAT 74 1.8499999999999999 No Hit GTTGAAACTAGCATATTGGAAGATTAATCGGCCAAAATAACCATGAGCAG 36 0.8999999999999999 No Hit GGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTACGTT 29 0.7250000000000001 No Hit GCGGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTACG 26 0.65 No Hit CTCATAAGGACCACCATTGTATAACCATTCATCAACGGATGCAGCTTCCC 24 0.6 No Hit GCCAAAATAACCATGAGCAGCCACAATATTATAAGTCTCTTCCTCTTGAC 24 0.6 No Hit CTTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCAT 23 0.575 No Hit CGCAGAAGTAGGAATAATAGCACCAGAGATAATATTGTTTCCATAAAGTA 18 0.44999999999999996 No Hit GGCCAAAATAACCATGAGCAGCCACAATATTATAAGTCTCTTCCTCTTGA 18 0.44999999999999996 No Hit TTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCATT 18 0.44999999999999996 No Hit TTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCATTAATA 18 0.44999999999999996 No Hit CTCGCGAATACCATCAATATCTACTGGAGGGGCTGCGATGAAGGCGATAA 18 0.44999999999999996 No Hit GCGGTCAATAAGGTAGGGATCATCAAAACACCGAACCATCCGATGTAAAG 16 0.4 No Hit CCCAAGTATTAATAACGCGACCTTGACTATCAACTACAGATTGGTTGAAA 16 0.4 No Hit GTGCTCTGCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTCCTA 16 0.4 No Hit CATATAACAAGCTACACCAAGTAAGAAGTGTAGAACAATTAGCTCATAAG 15 0.375 No Hit CGGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTACGT 14 0.35000000000000003 No Hit GGAAAAACCTTATCCATTAATAGCGGGAACTTCAAGAGCAGCTAGATCTA 14 0.35000000000000003 No Hit GCGGAAAAACCTTATCCATTAATAGCGGGAACTTCAAGAGCAGCTAGATC 14 0.35000000000000003 No Hit ACCAGCTACACCTAACATGTGAAATGGATGCATAAGGATGTTGTGCTCTG 13 0.325 No Hit ATCCGATTGCCGCAGAAGTAGGAATAATAGCACCAGAGATAATATTGTTT 13 0.325 No Hit CCAGATTCCTACTACAGGCCAAGCAGCCAAGAAGAAGTGTAAAGAACGAG 13 0.325 No Hit GCCGAAAACACCAGCTACACCTAACATGTGAAATGGATGCATAAGGATGT 12 0.3 No Hit GGTCAATAAGGTAGGGATCATCAAAACACCGAACCATCCGATGTAAAGAC 12 0.3 No Hit AAAAATTCTTATATGTTAGCGGAAAAACCTTATCCATTAATAGCGGGAAC 11 0.27499999999999997 No Hit CTTGACTATCAACTACAGATTGGTTGAAATTGAATCCATTTAGGTTGAAC 11 0.27499999999999997 No Hit GGCGCATACCCAAACGGAAACTAAGTTCCCACTCACGACCCATATAACAA 11 0.27499999999999997 No Hit CAAGTATTAATAACGCGACCTTGACTATCAACTACAGATTGGTTGAAATT 11 0.27499999999999997 No Hit GCTTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCA 11 0.27499999999999997 No Hit GTGAAATGGATGCATAAGGATGTTGTGCTCTGCCTGGAATACAATCATAA 11 0.27499999999999997 No Hit CGCGAATACCATCAATATCTACTGGAGGGGCTGCGATGAAGGCGATAATA 10 0.25 No Hit CGAGAGTTGTTGAAACTAGCATATTGGAAGATTAATCGGCCAAAATAACC 10 0.25 No Hit GTGAGCATTACGTTCGTGCATTACTTCCATACCAAGATTAGCACGGTTGA 10 0.25 No Hit GGATGCATAAGGATGTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAA 10 0.25 No Hit GCTAGTTATCCAGTTGCAGAAGCGACCCCACAGGCTTGTACTTTCGCGTC 10 0.25 No Hit CGGAAAAACCTTATCCATTAATAGCGGGAACTTCAAGAGCAGCTAGATCT 10 0.25 No Hit ATAACAAGCTACACCAAGTAAGAAGTGTAGAACAATTAGCTCATAAGGAC 10 0.25 No Hit GGAAGATTAATCGGCCAAAATAACCATGAGCAGCCACAATATTATAAGTC 9 0.22499999999999998 No Hit GGATCATCAAAACACCGAACCATCCGATGTAAAGACGATTTTCAGTGCTA 9 0.22499999999999998 No Hit CCACAATATTATAAGTCTCTTCCTCTTGACCAAATTTGTAACCCTCATTA 9 0.22499999999999998 No Hit CTCATTAGCAGATTCATTTTCAGTAGTTTCCCTGATCAAACTAGAGGTTA 9 0.22499999999999998 No Hit CTCTTGACCAAATTTGTAACCCTCATTAGCAGATTCATTTTCAGTAGTTT 9 0.22499999999999998 No Hit GCACGGTTGATGATATCAGCCCAAGTATTAATAACGCGACCTTGACTATC 8 0.2 No Hit GTCGCAGCTGCAACAGGAGCTGAATATGCAACAGCAATCCAAGGGCGCAT 8 0.2 No Hit ATAAGGACCACCATTGTATAACCATTCATCAACGGATGCAGCTTCCCAAA 8 0.2 No Hit GGGAAGTTGTGAGCATTACGTTCGTGCATTACTTCCATACCAAGATTAGC 8 0.2 No Hit GCATTACGTTCGTGCATTACTTCCATACCAAGATTAGCACGGTTGATGAT 8 0.2 No Hit AGTACTAATACCTAAAGCAGTGAACCAGATTCCTACTACAGGCCAAGCAG 8 0.2 No Hit GGCCAAGCAGCCAAGAAGAAGTGTAAAGAACGAGAGTTGTTGAAACTAGC 8 0.2 No Hit CATAAGGATGTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAAAGTAC 8 0.2 No Hit GCTAGATCTAGAGGGAAGTTGTGAGCATTACGTTCGTGCATTACTTCCAT 8 0.2 No Hit GGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTACGTTC 8 0.2 No Hit NTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCATTAAT 8 0.2 No Hit GGTAAATCAAGAAAACAGCAGTCGCAGCTGCAACAGGAGCTGAATATGCA 8 0.2 No Hit GCCAAGAAGAAGTGTAAAGAACGAGAGTTGTTGAAACTAGCATATTGGAA 8 0.2 No Hit CAATAAGGTAGGGATCATCAAAACACCGAACCATCCGATGTAAAGACGAT 7 0.17500000000000002 No Hit CAGAGAAGCTTCCTTGACCAATAGGGTAAATCAAGAAAACAGCAGTCGCA 7 0.17500000000000002 No Hit CAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCATTAATAGCGGGAA 7 0.17500000000000002 No Hit ACCAGATATTCCTAAAGGCATACCATCAGAGAAGCTTCCTTGACCAATAG 7 0.17500000000000002 No Hit GCTGAATATGCAACAGCAATCCAAGGGCGCATACCCAAACGGAAACTAAG 7 0.17500000000000002 No Hit GGCGATAATAAATACAGAAGTTGCGGTCAATAAGGTAGGGATCATCAAAA 7 0.17500000000000002 No Hit CCATATAACAAGCTACACCAAGTAAGAAGTGTAGAACAATTAGCTCATAA 7 0.17500000000000002 No Hit GCTGCGATGAAGGCGATAATAAATACAGAAGTTGCGGTCAATAAGGTAGG 7 0.17500000000000002 No Hit GCTACACCTAACATGTGAAATGGATGCATAAGGATGTTGTGCTCTGCCTG 7 0.17500000000000002 No Hit TTCCTCTTGACCAAATTTGTAACCCTCATTAGCAGATTCATTTTCAGTAG 7 0.17500000000000002 No Hit GTTCGCTGGAGTAGGCTGCGAGGCCCAAGCGGCCAAGGAGTGCAGCGGCC 7 0.17500000000000002 No Hit ATGATATCAGCCCAAGTATTAATAACGCGACCTTGACTATCAACTACAGA 7 0.17500000000000002 No Hit CCAAGTATTAATAACGCGACCTTGACTATCAACTACAGATTGGTTGAAAT 7 0.17500000000000002 No Hit GCATAAGGATGTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAAAGTA 7 0.17500000000000002 No Hit GTGCTAGTTATCCAGTTGCAGAAGCGACCCCACAGGCTTGTACTTTCGCG 7 0.17500000000000002 No Hit ATATAACAAGCTACACCAAGTAAGAAGTGTAGAACAATTAGCTCATAAGG 7 0.17500000000000002 No Hit GACCCATATAACAAGCTACACCAAGTAAGAAGTGTAGAACAATTAGCTCA 7 0.17500000000000002 No Hit GCTCTGCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTCCTAAA 7 0.17500000000000002 No Hit GTGCAATCCGATTGCCGCAGAAGTAGGAATAATAGCACCAGAGATAATAT 6 0.15 No Hit TAAGGATGTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAAAGTACCA 6 0.15 No Hit CCCATATAACAAGCTACACCAAGTAAGAAGTGTAGAACAATTAGCTCATA 6 0.15 No Hit GTATTAATAACGCGACCTTGACTATCAACTACAGATTGGTTGAAATTGAA 6 0.15 No Hit GTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTC 6 0.15 No Hit GGAGGGGCTGCGATGAAGGCGATAATAAATACAGAAGTTGCGGTCAATAA 6 0.15 No Hit TTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCATTA 6 0.15 No Hit TCTTGACCAAATTTGTAACCCTCATTAGCAGATTCATTTTCAGTAGTTTC 6 0.15 No Hit GCACTGAATAGGGAACCGCCGAAAACACCAGCTACACCTAACATGTGAAA 6 0.15 No Hit CCCTCATTAGCAGATTCATTTTCAGTAGTTTCCCTGATCAAACTAGAGGT 6 0.15 No Hit CATCAGAGAAGCTTCCTTGACCAATAGGGTAAATCAAGAAAACAGCAGTC 6 0.15 No Hit GAAAAACCTTATCCATTAATAGCGGGAACTTCAAGAGCAGCTAGATCTAG 6 0.15 No Hit GAGCTGAATATGCAACAGCAATCCAAGGGCGCATACCCAAACGGAAACTA 6 0.15 No Hit AGCGGAAAAACCTTATCCATTAATAGCGGGAACTTCAAGAGCAGCTAGAT 6 0.15 No Hit CACCATTGTATAACCATTCATCAACGGATGCAGCTTCCCAAATTGGGTAA 6 0.15 No Hit CTGGAATACAATCATAAAGTTGAAAGTACCAGATATTCCTAAAGGCATAC 6 0.15 No Hit CCTCATTAGCAGATTCATTTTCAGTAGTTTCCCTGATCAAACTAGAGGTT 6 0.15 No Hit GAGAAGCTTCCTTGACCAATAGGGTAAATCAAGAAAACAGCAGTCGCAGC 5 0.125 No Hit GCGATAATAAATACAGAAGTTGCGGTCAATAAGGTAGGGATCATCAAAAC 5 0.125 No Hit ATTACGTTCGTGCATTACTTCCATACCAAGATTAGCACGGTTGATGATAT 5 0.125 No Hit ATCAGAGAAGCTTCCTTGACCAATAGGGTAAATCAAGAAAACAGCAGTCG 5 0.125 No Hit NTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCATT 5 0.125 No Hit CCACCATTGTATAACCATTCATCAACGGATGCAGCTTCCCAAATTGGGTA 5 0.125 No Hit GCATATTGGAAGATTAATCGGCCAAAATAACCATGAGCAGCCACAATATT 5 0.125 No Hit GGGATCATCAAAACACCGAACCATCCGATGTAAAGACGATTTTCAGTGCT 5 0.125 No Hit CACTCACGACCCATATAACAAGCTACACCAAGTAAGAAGTGTAGAACAAT 5 0.125 No Hit TGAAATTGAATCCATTTAGGTTGAACGCCATAGTACTAATACCTAAAGCA 5 0.125 No Hit CCATTGTATAACCATTCATCAACGGATGCAGCTTCCCAAATTGGGTAAAA 5 0.125 No Hit CTACAGGCCAAGCAGCCAAGAAGAAGTGTAAAGAACGAGAGTTGTTGAAA 5 0.125 No Hit ATCAATATCTACTGGAGGGGCTGCGATGAAGGCGATAATAAATACAGAAG 5 0.125 No Hit AAAGAACGAGAGTTGTTGAAACTAGCATATTGGAAGATTAATCGGCCAAA 5 0.125 No Hit TAAAGAACGAGAGTTGTTGAAACTAGCATATTGGAAGATTAATCGGCCAA 5 0.125 No Hit TGACTATCAACTACAGATTGGTTGAAATTGAATCCATTTAGGTTGAACGC 5 0.125 No Hit ATTAATAGCGGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAG 5 0.125 No Hit CAAGAAGAAGTGTAAAGAACGAGAGTTGTTGAAACTAGCATATTGGAAGA 5 0.125 No Hit CCATGAGCAGCCACAATATTATAAGTCTCTTCCTCTTGACCAAATTTGTA 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0125 0.0 0.0 0.0 0.0 66-67 0.025 0.0 0.0 0.0 0.0 68-69 0.025 0.0 0.0 0.0 0.0 70-71 0.025 0.0 0.0 0.0 0.0 72-73 0.037500000000000006 0.0 0.0 0.0 0.0 74-75 0.1375 0.0 0.0 0.0 0.0 76-77 0.175 0.0 0.0 0.0 0.0 78-79 0.25 0.0 0.0 0.0 0.0 80-81 0.325 0.0 0.0 0.0 0.0 82-83 0.48750000000000004 0.0 0.0 0.0 0.0 84-85 0.5375000000000001 0.0 0.0 0.0 0.0 86-87 0.6875 0.0 0.0 0.0 0.0 88-89 0.8125 0.0 0.0 0.0 0.0 90-91 0.875 0.0 0.0 0.0 0.0 92-93 1.1 0.0 0.0 0.0 0.0 94-95 1.35 0.0 0.0 0.0 0.0 96-97 1.4874999999999998 0.0 0.0 0.0 0.0 98-99 1.6749999999999998 0.0 0.0 0.0 0.0 100-101 1.875 0.0 0.0 0.0 0.0 102-103 2.1375 0.0 0.0 0.0 0.0 104-105 2.35 0.0 0.0 0.0 0.0 106-107 2.625 0.0 0.0 0.0 0.0 108-109 3.1625 0.0 0.0 0.0 0.0 110-111 3.425 0.0 0.0 0.0 0.0 112-113 3.95 0.0 0.0 0.0 0.0 114-115 4.375 0.0 0.0 0.0 0.0 116-117 4.8875 0.0 0.0 0.0 0.0 118-119 5.3875 0.0 0.0 0.0 0.0 120-121 5.9625 0.0 0.0 0.0 0.0 122-123 6.5375 0.0 0.0 0.0 0.0 124-125 7.125 0.0 0.0 0.0 0.0 126-127 7.875 0.0 0.0 0.0 0.0 128-129 8.5625 0.0 0.0 0.0 0.0 130-131 9.25 0.0 0.0 0.0 0.0 132-133 10.1 0.0 0.0 0.0 0.0 134-135 10.9125 0.0 0.0 0.0 0.0 136-137 11.825 0.0 0.0 0.0 0.0 138-139 12.6 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGTCTTC 10 0.0068396386 144.9375 145 CCATATA 40 0.0056309355 54.351562 2 CATATAA 45 0.008972557 48.3125 3 >>END_MODULE SRR6941591 read2 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR6941591_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.978 33.0 33.0 34.0 32.0 34.0 2 33.03875 34.0 33.0 34.0 32.0 34.0 3 33.01225 34.0 33.0 34.0 32.0 34.0 4 33.00675 34.0 33.0 34.0 33.0 34.0 5 33.00575 34.0 33.0 34.0 33.0 34.0 6 37.1875 38.0 38.0 38.0 37.0 38.0 7 37.081 38.0 38.0 38.0 37.0 38.0 8 37.15875 38.0 38.0 38.0 37.0 38.0 9 37.066 38.0 38.0 38.0 37.0 38.0 10-14 36.7673 38.0 38.0 38.0 35.4 38.0 15-19 36.95695 38.0 38.0 38.0 36.4 38.0 20-24 37.089999999999996 38.0 38.0 38.0 37.0 38.0 25-29 36.97555 38.0 38.0 38.0 36.6 38.0 30-34 36.5809 38.0 38.0 38.0 34.8 38.0 35-39 36.4454 38.0 38.0 38.0 34.6 38.0 40-44 36.86605000000001 38.0 38.0 38.0 35.8 38.0 45-49 36.96105 38.0 38.0 38.0 36.8 38.0 50-54 36.93275 38.0 38.0 38.0 36.0 38.0 55-59 36.7823 38.0 38.0 38.0 35.8 38.0 60-64 36.907000000000004 38.0 38.0 38.0 36.2 38.0 65-69 36.75275 38.0 38.0 38.0 35.6 38.0 70-74 36.53580000000001 38.0 38.0 38.0 35.0 38.0 75-79 36.66875 38.0 38.0 38.0 35.4 38.0 80-84 36.4517 38.0 38.0 38.0 34.6 38.0 85-89 36.1909 38.0 37.8 38.0 33.2 38.0 90-94 36.566 38.0 38.0 38.0 35.0 38.0 95-99 36.43194999999999 38.0 38.0 38.0 34.4 38.0 100-104 35.536649999999995 38.0 37.0 38.0 30.2 38.0 105-109 35.7771 38.0 37.0 38.0 32.0 38.0 110-114 36.201299999999996 38.0 38.0 38.0 34.0 38.0 115-119 36.13195 38.0 38.0 38.0 34.0 38.0 120-124 35.7414 38.0 37.4 38.0 32.4 38.0 125-129 35.36395 38.0 36.4 38.0 30.0 38.0 130-134 35.3835 38.0 36.2 38.0 31.8 38.0 135-139 31.456300000000006 36.2 25.2 38.0 20.2 38.0 140-144 33.15435 37.0 31.2 38.0 22.6 38.0 145-149 34.0706 38.0 35.0 38.0 25.8 38.0 150-151 29.393875 35.0 18.5 38.0 7.5 38.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6.0 3 11.0 4 4.0 5 2.0 6 0.0 7 1.0 8 2.0 9 3.0 10 0.0 11 3.0 12 0.0 13 3.0 14 5.0 15 2.0 16 1.0 17 3.0 18 0.0 19 5.0 20 4.0 21 9.0 22 7.0 23 6.0 24 9.0 25 18.0 26 20.0 27 26.0 28 35.0 29 37.0 30 42.0 31 59.0 32 80.0 33 101.0 34 151.0 35 251.0 36 777.0 37 2317.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 37.275000000000006 20.8 18.25 23.674999999999997 2 25.894420815611706 19.88991743807856 37.50312734550913 16.7125344008006 3 18.828242363545318 23.885828743114672 38.38257386079118 18.903355032548824 4 22.84784784784785 31.306306306306308 26.45145145145145 19.394394394394393 5 24.44944944944945 31.556556556556558 27.127127127127125 16.866866866866868 6 19.650000000000002 33.375 29.425 17.549999999999997 7 17.125 18.525 46.025 18.325 8 19.325 22.6 32.475 25.6 9 21.9 21.125 35.175 21.8 10-14 23.369999999999997 25.169999999999998 31.36 20.1 15-19 23.225 24.465 32.824999999999996 19.485 20-24 24.055 24.41 32.53 19.005 25-29 23.50735073507351 24.45244524452445 31.588158815881588 20.452045204520452 30-34 23.98479695939188 24.25485097019404 31.501300260052012 20.259051810362074 35-39 23.087308730873087 24.922492249224923 31.753175317531753 20.237023702370237 40-44 22.79 25.97 31.619999999999997 19.62 45-49 22.291687506251876 26.778033410023006 30.854256276883063 20.076022806842055 50-54 22.092209220922093 26.207620762076207 31.038103810381042 20.662066206620665 55-59 22.09 25.72 30.69 21.5 60-64 22.747274727472746 24.432443244324435 31.62816281628163 21.19211921192119 65-69 23.071921576472942 25.177553265979796 30.86926077823347 20.881264379313794 70-74 23.817145143543065 25.027508252475744 31.439431829548862 19.71591477443233 75-79 23.77737773777378 24.817481748174817 30.97809780978098 20.427042704270427 80-84 23.98739873987399 24.387438743874387 32.45324532453245 19.171917191719174 85-89 23.715 25.915 29.515 20.855 90-94 23.435 25.290000000000003 30.42 20.855 95-99 22.939999999999998 25.169999999999998 31.424999999999997 20.465 100-104 24.37 24.72 31.155 19.755 105-109 24.85 23.765 30.990000000000002 20.395 110-114 23.477347734773478 24.877487748774875 31.248124812481247 20.397039703970396 115-119 24.167416741674167 25.152515251525152 30.683068306830684 19.996999699969997 120-124 23.2973297329733 26.53765376537654 29.44294429442944 20.72207220722072 125-129 23.77237723772377 26.0976097609761 29.82298229822982 20.307030703070307 130-134 24.065 26.0 30.435000000000002 19.5 135-139 24.665 26.490000000000002 30.035 18.81 140-144 25.617561756175615 25.542554255425543 30.06800680068007 18.771877187718772 145-149 25.074999999999996 25.415 30.259999999999998 19.25 150-151 24.6 25.0 31.5 18.9 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.5 13 0.5 14 0.0 15 0.0 16 0.5 17 0.5 18 0.5 19 1.0 20 1.0 21 2.0 22 3.5 23 4.5 24 5.0 25 7.0 26 8.5 27 15.5 28 20.0 29 22.0 30 26.5 31 30.5 32 35.5 33 37.5 34 56.5 35 81.5 36 107.0 37 173.0 38 207.0 39 214.5 40 273.0 41 285.0 42 251.0 43 276.5 44 296.0 45 237.5 46 201.0 47 168.5 48 124.0 49 100.5 50 77.0 51 73.0 52 56.5 53 40.0 54 46.5 55 49.5 56 48.5 57 42.5 58 40.5 59 41.5 60 34.5 61 30.5 62 28.5 63 26.5 64 23.0 65 14.5 66 11.0 67 9.0 68 8.0 69 9.0 70 5.0 71 4.0 72 2.5 73 0.5 74 0.0 75 0.5 76 1.5 77 1.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.075 3 0.15 4 0.1 5 0.1 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.01 30-34 0.02 35-39 0.01 40-44 0.0 45-49 0.03 50-54 0.01 55-59 0.0 60-64 0.01 65-69 0.03 70-74 0.03 75-79 0.01 80-84 0.01 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.01 115-119 0.01 120-124 0.01 125-129 0.01 130-134 0.0 135-139 0.0 140-144 0.01 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 63.7 #Duplication Level Percentage of deduplicated Percentage of total 1 82.574568288854 52.6 2 6.63265306122449 8.450000000000001 3 3.767660910518053 7.199999999999999 4 1.9230769230769231 4.9 5 1.7660910518053377 5.625 6 0.7064364207221351 2.7 7 0.5886970172684459 2.625 8 0.47095761381475665 2.4 9 0.23547880690737832 1.35 >10 1.3343799058084773 12.15 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source ATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAA 40 1.0 No Hit GTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGATCTT 26 0.65 No Hit GCTTGTTATATGGGTCGTGAGTGGGAACTTAGTTTCCGTTTGGGTATGCG 20 0.5 No Hit GCACAACATCCTTATGCATCCATTTCACATGTTAGGTGTAGCTGGTGTTT 20 0.5 No Hit CTATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAA 18 0.44999999999999996 No Hit TTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAG 16 0.4 No Hit ATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCA 16 0.4 No Hit TATCTATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTT 16 0.4 No Hit TTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAA 15 0.375 No Hit GGAAACTACTGAAAATGAATCTGCTAATGAGGGTTACAAATTTGGTCAAG 15 0.375 No Hit TGTAGCTGGTGTTTTCGGCGGTTCCCTATTCAGTGCTATGCATGGTTCCT 15 0.375 No Hit AGCACAACATCCTTATGCATCCATTTCACATGTTAGGTGTAGCTGGTGTT 14 0.35000000000000003 No Hit TATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAA 14 0.35000000000000003 No Hit GCTGCGACTGCTGTTTTCTTGATTTACCCTATTGGTCAAGGAAGCTTCTC 14 0.35000000000000003 No Hit GGTACTTTCAACTTTATGATTGTATTCCAGGCAGAGCACAACATCCTTAT 14 0.35000000000000003 No Hit GGTCGCTTCTGCAACTGGATAACTAGCACTGAAAATCGTCTTTACATCGG 13 0.325 No Hit CATCAACCGTGCTAATCTTGGTATGGAAGTAATGCACGAACGTAATGCTC 13 0.325 No Hit GTTTGATCAGGGAAACTACTGAAAATGAATCTGCTAATGAGGGTTACAAA 13 0.325 No Hit CCAAGATCTTACCATGACTGCAATTTTAGAGAGACGCGAAAGTACAAGCC 12 0.3 No Hit GTTATTTTGGCCGATTAATCTTCCAATATGCTAGTTTCAACAACTCTCGT 12 0.3 No Hit ATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCTAAATGG 12 0.3 No Hit GGAAGCTTCTCTGATGGTATGCCTTTAGGAATATCTGGTACTTTCAACTT 12 0.3 No Hit GTGGGAACTTAGTTTCCGTTTGGGTATGCGCCCTTGGATTGCTGTTGCAT 12 0.3 No Hit CCGCAACTTCTGTATTTATTATCGCCTTCATCGCAGCCCCTCCAGTAGAT 11 0.27499999999999997 No Hit ATCGGATTGCACTTTTACCCAATTTGGGAAGCTGCATCCGTTGATGAATG 11 0.27499999999999997 No Hit GTCAAGGTCGCGTTATTAATACTTGGGCTGATATCATCAACCGTGCTAAT 11 0.27499999999999997 No Hit TCTATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGA 11 0.27499999999999997 No Hit CCTACTTCTGCGGCAATCGGATTGCACTTTTACCCAATTTGGGAAGCTGC 10 0.25 No Hit ATTTGGTCAAGAGGAAGAGACTTATAATATTGTGGCTGCTCATGGTTATT 10 0.25 No Hit GTTGCATATTCAGCTCCTGTTGCAGCTGCGACTGCTGTTTTCTTGATTTA 10 0.25 No Hit GGGAACTTAGTTTCCGTTTGGGTATGCGCCCTTGGATTGCTGTTGCATAT 10 0.25 No Hit ATTATCTATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAAT 10 0.25 No Hit GTTCAACCTAAATGGATTCAATTTCAACCAATCTGTAGTTGATAGTCAAG 10 0.25 No Hit GCTTTAGGTATTAGTACTATGGCGTTCAACCTAAATGGATTCAATTTCAA 10 0.25 No Hit ATTATTCCTACTTCTGCGGCAATCGGATTGCACTTTTACCCAATTTGGGA 9 0.22499999999999998 No Hit CTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGAT 9 0.22499999999999998 No Hit GTTTTGATGATCCCTACCTTATTGACCGCAACTTCTGTATTTATTATCGC 9 0.22499999999999998 No Hit TGATGGTATTCGCGAGCCTGTTTCTGGTTCTTTACTTTATGGAAACAATA 9 0.22499999999999998 No Hit CGAAAGTACAAGCCTGTGGGGTCGCTTCTGCAACTGGATAACTAGCACTG 9 0.22499999999999998 No Hit GTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGATCTTACCATGAC 9 0.22499999999999998 No Hit TGGTAACCTCTAGTTTGATCAGGGAAACTACTGAAAATGAATCTGCTAAT 8 0.2 No Hit CTGGTACTTTCAACTTTATGATTGTATTCCAGGCAGAGCACAACATCCTT 8 0.2 No Hit GGGAAACTACTGAAAATGAATCTGCTAATGAGGGTTACAAATTTGGTCAA 8 0.2 No Hit CCTATTCAGTGCTATGCATGGTTCCTTGGTAACCTCTAGTTTGATCAGGG 8 0.2 No Hit GGAATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCTAAA 8 0.2 No Hit GGAACTTAGTTTCCGTTTGGGTATGCGCCCTTGGATTGCTGTTGCATATT 8 0.2 No Hit GTAACCTCTAGTTTGATCAGGGAAACTACTGAAAATGAATCTGCTAATGA 8 0.2 No Hit CACATGTTAGGTGTAGCTGGTGTTTTCGGCGGTTCCCTATTCAGTGCTAT 8 0.2 No Hit GTTAGGTGTAGCTGGTGTTTTCGGCGGTTCCCTATTCAGTGCTATGCATG 8 0.2 No Hit AACATCCTTATGCATCCATTTCACATGTTAGGTGTAGCTGGTGTTTTCGG 8 0.2 No Hit CTTCCAATATGCTAGTTTCAACAACTCTCGTTCTTTACACTTCTTCTTGG 8 0.2 No Hit TGATAGTCAAGGTCGCGTTATTAATACTTGGGCTGATATCATCAACCGTG 8 0.2 No Hit GCTAATTGTTCTACACTTCTTACTTGGTGTAGCTTGTTATATGGGTCGTG 7 0.17500000000000002 No Hit GTTTTATGGACCCACCGGCCCAGAAGCTTCTCAAGCTCAAGCATTTACTT 7 0.17500000000000002 No Hit GAGACTTATAATATTGTGGCTGCTCATGGTTATTTTGGCCGATTAATCTT 7 0.17500000000000002 No Hit TGAAAATGAATCTGCTAATGAGGGTTACAAATTTGGTCAAGAGGAAGAGA 7 0.17500000000000002 No Hit CTTTACTTTATGGAAACAATATTATCTCTGGTGCTATTATTCCTACTTCT 7 0.17500000000000002 No Hit GCATGGTTCCTTGGTAACCTCTAGTTTGATCAGGGAAACTACTGAAAATG 7 0.17500000000000002 No Hit CTTAGTTTCCGTTTGGGTATGCGCCCTTGGATTGCTGTTGCATATTCAGC 7 0.17500000000000002 No Hit TGGGAGTCCTTGCAATTTGAATAAACCAAGATCTTACCATGACTGCAATT 7 0.17500000000000002 No Hit TCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGA 7 0.17500000000000002 No Hit GTTGATGAATGGTTATACAATGGTGGTCCTTATGAGCTAATTGTTCTACA 7 0.17500000000000002 No Hit GCTGCATCCGTTGATGAATGGTTATACAATGGTGGTCCTTATGAGCTAAT 7 0.17500000000000002 No Hit TTTAGGAATATCTGGTACTTTCAACTTTATGATTGTATTCCAGGCAGAGC 7 0.17500000000000002 No Hit GTACAAGCCTGTGGGGTCGCTTCTGCAACTGGATAACTAGCACTGAAAAT 7 0.17500000000000002 No Hit ATCTATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTG 7 0.17500000000000002 No Hit GTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGATCTTACCATGACTG 7 0.17500000000000002 No Hit GTTTGGGTATGCGCCCTTGGATTGCTGTTGCATATTCAGCTCCTGTTGCA 6 0.15 No Hit GAAAGTACAAGCCTGTGGGGTCGCTTCTGCAACTGGATAACTAGCACTGA 6 0.15 No Hit CTCGTTCTTTACACTTCTTCTTGGCTGCTTGGCCTGTAGTAGGAATCTGG 6 0.15 No Hit CCTAAATGGATTCAATTTCAACCAATCTGTAGTTGATAGTCAAGGTCGCG 6 0.15 No Hit TGGGAACTTAGTTTCCGTTTGGGTATGCGCCCTTGGATTGCTGTTGCATA 6 0.15 No Hit CTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCTAAATGGAT 6 0.15 No Hit ATAATATTGTGGCTGCTCATGGTTATTTTGGCCGATTAATCTTCCAATAT 6 0.15 No Hit CGCAGCCCCTCCAGTAGATATTGATGGTATTCGCGAGCCTGTTTCTGGTT 6 0.15 No Hit AGACGCGAAAGTACAAGCCTGTGGGGTCGCTTCTGCAACTGGATAACTAG 6 0.15 No Hit ATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATA 6 0.15 No Hit GTTCGATCCTGGCTCAGGATGAACGCTGGCGGCATGCTTAACACATGCAA 6 0.15 No Hit GCCTTTAGGAATATCTGGTACTTTCAACTTTATGATTGTATTCCAGGCAG 6 0.15 No Hit GTTTCAACAACTCTCGTTCTTTACACTTCTTCTTGGCTGCTTGGCCTGTA 6 0.15 No Hit ATTCAGCTCCTGTTGCAGCTGCGACTGCTGTTTTCTTGATTTACCCTATT 6 0.15 No Hit GTCCTTGCAATTTGAATAAACCAAGATCTTACCATGACTGCAATTTTAGA 6 0.15 No Hit TTTAGAGAGACGCGAAAGTACAAGCCTGTGGGGTCGCTTCTGCAACTGGA 6 0.15 No Hit ATACAATGGTGGTCCTTATGAGCTAATTGTTCTACACTTCTTACTTGGTG 6 0.15 No Hit CGCAACTTCTGTATTTATTATCGCCTTCATCGCAGCCCCTCCAGTAGATA 6 0.15 No Hit CTATTCAGTGCTATGCATGGTTCCTTGGTAACCTCTAGTTTGATCAGGGA 5 0.125 No Hit CGGCGGTTCCCTATTCAGTGCTATGCATGGTTCCTTGGTAACCTCTAGTT 5 0.125 No Hit CTTGGGAGTCCTTGCAATTTGAATAAACCAAGATCTTACCATGACTGCAA 5 0.125 No Hit GGCGTTCAACCTAAATGGATTCAATTTCAACCAATCTGTAGTTGATAGTC 5 0.125 No Hit TAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGATC 5 0.125 No Hit ATTCCTACTTCTGCGGCAATCGGATTGCACTTTTACCCAATTTGGGAAGC 5 0.125 No Hit TTCACATGTTAGGTGTAGCTGGTGTTTTCGGCGGTTCCCTATTCAGTGCT 5 0.125 No Hit GTTTTCGGCGGTTCCCTATTCAGTGCTATGCATGGTTCCTTGGTAACCTC 5 0.125 No Hit CTTTTACCCAATTTGGGAAGCTGCATCCGTTGATGAATGGTTATACAATG 5 0.125 No Hit GTCGCTTCTGCAACTGGATAACTAGCACTGAAAATCGTCTTTACATCGGA 5 0.125 No Hit ATTTCAACCAATCTGTAGTTGATAGTCAAGGTCGCGTTATTAATACTTGG 5 0.125 No Hit ATATCATCAACCGTGCTAATCTTGGTATGGAAGTAATGCACGAACGTAAT 5 0.125 No Hit TATTGATGGTATTCGCGAGCCTGTTTCTGGTTCTTTACTTTATGGAAACA 5 0.125 No Hit ATCGCCTTCATCGCAGCCCCTCCAGTAGATATTGATGGTATTCGCGAGCC 5 0.125 No Hit GTTCCTTGGTAACCTCTAGTTTGATCAGGGAAACTACTGAAAATGAATCT 5 0.125 No Hit CTTCTGTATTTATTATCGCCTTCATCGCAGCCCCTCCAGTAGATATTGAT 5 0.125 No Hit GCAATTTTAGAGAGACGCGAAAGTACAAGCCTGTGGGGTCGCTTCTGCAA 5 0.125 No Hit GAGGGTTACAAATTTGGTCAAGAGGAAGAGACTTATAATATTGTGGCTGC 5 0.125 No Hit GGAATATCTGGTACTTTCAACTTTATGATTGTATTCCAGGCAGAGCACAA 5 0.125 No Hit CTATTATCTATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCA 5 0.125 No Hit CGTTGATGAATGGTTATACAATGGTGGTCCTTATGAGCTAATTGTTCTAC 5 0.125 No Hit AGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGATCT 5 0.125 No Hit CTGCTAATGAGGGTTACAAATTTGGTCAAGAGGAAGAGACTTATAATATT 5 0.125 No Hit CTTGTTATATGGGTCGTGAGTGGGAACTTAGTTTCCGTTTGGGTATGCGC 5 0.125 No Hit AATGGATTCAATTTCAACCAATCTGTAGTTGATAGTCAAGGTCGCGTTAT 5 0.125 No Hit AAACAATATTATCTCTGGTGCTATTATTCCTACTTCTGCGGCAATCGGAT 5 0.125 No Hit ATTATCTCTGGTGCTATTATTCCTACTTCTGCGGCAATCGGATTGCACTT 5 0.125 No Hit GTTATACAATGGTGGTCCTTATGAGCTAATTGTTCTACACTTCTTACTTG 5 0.125 No Hit ATGCGCCCTTGGATTGCTGTTGCATATTCAGCTCCTGTTGCAGCTGCGAC 5 0.125 No Hit AGTCAAGGTCGCGTTATTAATACTTGGGCTGATATCATCAACCGTGCTAA 5 0.125 No Hit GTAGCTTGTTATATGGGTCGTGAGTGGGAACTTAGTTTCCGTTTGGGTAT 5 0.125 No Hit GTAGGAATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCT 5 0.125 No Hit CAACAACTCTCGTTCTTTACACTTCTTCTTGGCTGCTTGGCCTGTAGTAG 5 0.125 No Hit CTCTGGTGCTATTATTCCTACTTCTGCGGCAATCGGATTGCACTTTTACC 5 0.125 No Hit CAATGGTGGTCCTTATGAGCTAATTGTTCTACACTTCTTACTTGGTGTAG 5 0.125 No Hit TTTGAATAAACCAAGATCTTACCATGACTGCAATTTTAGAGAGACGCGAA 5 0.125 No Hit GTTTCTGGTTCTTTACTTTATGGAAACAATATTATCTCTGGTGCTATTAT 5 0.125 No Hit TCGGATTGCACTTTTACCCAATTTGGGAAGCTGCATCCGTTGATGAATGG 5 0.125 No Hit GTCAAGAGGAAGAGACTTATAATATTGTGGCTGCTCATGGTTATTTTGGC 5 0.125 No Hit GTGTTTTCGGCGGTTCCCTATTCAGTGCTATGCATGGTTCCTTGGTAACC 5 0.125 No Hit GGCCGATTAATCTTCCAATATGCTAGTTTCAACAACTCTCGTTCTTTACA 5 0.125 No Hit CTGAAAATGAATCTGCTAATGAGGGTTACAAATTTGGTCAAGAGGAAGAG 5 0.125 No Hit TTTACATCGGATGGTTCGGTGTTTTGATGATCCCTACCTTATTGACCGCA 5 0.125 No Hit GCCGATTAATCTTCCAATATGCTAGTTTCAACAACTCTCGTTCTTTACAC 5 0.125 No Hit CAACATCCTTATGCATCCATTTCACATGTTAGGTGTAGCTGGTGTTTTCG 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0125 0.0 0.0 0.0 0.0 66-67 0.025 0.0 0.0 0.0 0.0 68-69 0.025 0.0 0.0 0.0 0.0 70-71 0.025 0.0 0.0 0.0 0.0 72-73 0.037500000000000006 0.0 0.0 0.0 0.0 74-75 0.1375 0.0 0.0 0.0 0.0 76-77 0.175 0.0 0.0 0.0 0.0 78-79 0.25 0.0 0.0 0.0 0.0 80-81 0.325 0.0 0.0 0.0 0.0 82-83 0.44999999999999996 0.0 0.0 0.0 0.0 84-85 0.5 0.0 0.0 0.0 0.0 86-87 0.6499999999999999 0.0 0.0 0.0 0.0 88-89 0.7625 0.0 0.0 0.0 0.0 90-91 0.825 0.0 0.0 0.0 0.0 92-93 1.0375 0.0 0.0 0.0 0.0 94-95 1.2625000000000002 0.0 0.0 0.0 0.0 96-97 1.375 0.0 0.0 0.0 0.0 98-99 1.5499999999999998 0.0 0.0 0.0 0.0 100-101 1.7625000000000002 0.0 0.0 0.0 0.0 102-103 2.025 0.0 0.0 0.0 0.0 104-105 2.275 0.0 0.0 0.0 0.0 106-107 2.525 0.0 0.0 0.0 0.0 108-109 3.0875 0.0 0.0 0.0 0.0 110-111 3.35 0.0 0.0 0.0 0.0 112-113 3.875 0.0 0.0 0.0 0.0 114-115 4.300000000000001 0.0 0.0 0.0 0.0 116-117 4.7875 0.0 0.0 0.0 0.0 118-119 5.2875 0.0 0.0 0.0 0.0 120-121 5.8375 0.0 0.0 0.0 0.0 122-123 6.4125 0.0 0.0 0.0 0.0 124-125 7.0 0.0 0.0 0.0 0.0 126-127 7.6625 0.0 0.0 0.0 0.0 128-129 8.2375 0.0 0.0 0.0 0.0 130-131 8.8125 0.0 0.0 0.0 0.0 132-133 9.475000000000001 0.0 0.0 0.0 0.0 134-135 10.05 0.0 0.0 0.0 0.0 136-137 10.7875 0.0 0.0 0.0 0.0 138-139 11.462499999999999 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content pass >>END_MODULE Read 1228987 spots for SRR6941591.sra Written 1228987 spots for SRR6941591.sra Read 1228987 spots for SRR6941591.sra Written 1228987 spots for SRR6941591.sra Read 1228987 spots for SRR6941591.sra Written 1228987 spots for SRR6941591.sra Read 1228987 spots for SRR6941591.sra Written 1228987 spots for SRR6941591.sra Read 1228987 spots for SRR6941591.sra Written 1228987 spots for SRR6941591.sra Read 1228987 spots for SRR6941591.sra Written 1228987 spots for SRR6941591.sra Read 1228987 spots for SRR6941591.sra Written 1228987 spots for SRR6941591.sra Read 1228987 spots for SRR6941591.sra Written 1228987 spots for SRR6941591.sra Read 1228987 spots for SRR6941591.sra Written 1228987 spots for SRR6941591.sra Read 1228987 spots for SRR6941591.sra Written 1228987 spots for SRR6941591.sra Read 1229001 spots for SRR6941591.sra Written 1229001 spots for SRR6941591.sra Read 1228987 spots for SRR6941591.sra Written 1228987 spots for SRR6941591.sra Read 1228987 spots for SRR6941591.sra Written 1228987 spots for SRR6941591.sra Read 1228987 spots for SRR6941591.sra Written 1228987 spots for SRR6941591.sra Read 1228987 spots for SRR6941591.sra Written 1228987 spots for SRR6941591.sra Read 1228987 spots for SRR6941591.sra Written 1228987 spots for SRR6941591.sra Read 1228987 spots for SRR6941591.sra Written 1228987 spots for SRR6941591.sra Read 1228987 spots for SRR6941591.sra Written 1228987 spots for SRR6941591.sra Read 1228987 spots for SRR6941591.sra Written 1228987 spots for SRR6941591.sra Read 1228987 spots for SRR6941591.sra Written 1228987 spots for SRR6941591.sra SRR ids: ['SRR6941591.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_1zq5lm6h SRR6941591.sra spots: 24579754 blocks: [[1, 1228987], [1228988, 2457974], [2457975, 3686961], [3686962, 4915948], [4915949, 6144935], [6144936, 7373922], [7373923, 8602909], [8602910, 9831896], [9831897, 11060883], [11060884, 12289870], [12289871, 13518857], [13518858, 14747844], [14747845, 15976831], [15976832, 17205818], [17205819, 18434805], [18434806, 19663792], [19663793, 20892779], [20892780, 22121766], [22121767, 23350753], [23350754, 24579754]] SRR6941591 file size 8307571 SRR6941591 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR6941591 SRR6941591_1.fastq SRR6941591_2.fastq Input file: SRR6941591_1.fastq Paired file: SRR6941591_2.fastq trimmed: SRR6941591-trimmed-pair1.fastq, SRR6941591-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Fri Dec 6 12:23:14 2024 >> started Fri Dec 6 12:23:43 2024 >> done (28.860s) 24579754 read pairs processed; of these: 45963 ( 0.19%) short read pairs filtered out after trimming by size control 33903 ( 0.14%) empty read pairs filtered out after trimming by size control 24499888 (99.68%) read pairs available; of these: 10008112 (40.85%) trimmed read pairs available after processing 14491776 (59.15%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 2 0.00% 19 2 0.00% 20 4 0.00% 21 6 0.00% 22 8 0.00% 23 4 0.00% 24 5 0.00% 25 2 0.00% 26 3 0.00% 27 20 0.00% 28 1 0.00% 29 6 0.00% 30 12 0.00% 31 5 0.00% 32 8 0.00% 33 7 0.00% 34 11 0.00% 35 14 0.00% 36 8 0.00% 37 11 0.00% 38 17 0.00% 39 19 0.00% 40 21 0.00% 41 31 0.00% 42 32 0.00% 43 43 0.00% 44 40 0.00% 45 43 0.00% 46 56 0.00% 47 66 0.00% 48 62 0.00% 49 78 0.00% 50 133 0.00% 51 152 0.00% 52 193 0.00% 53 207 0.00% 54 233 0.00% 55 252 0.00% 56 273 0.00% 57 277 0.00% 58 381 0.00% 59 413 0.00% 60 514 0.00% 61 607 0.00% 62 881 0.00% 63 860 0.00% 64 1118 0.00% 65 1173 0.00% 66 1259 0.01% 67 1333 0.01% 68 1585 0.01% 69 1753 0.01% 70 2098 0.01% 71 2674 0.01% 72 3221 0.01% 73 3525 0.01% 74 3830 0.02% 75 4457 0.02% 76 4736 0.02% 77 5168 0.02% 78 5637 0.02% 79 6630 0.03% 80 7782 0.03% 81 8968 0.04% 82 9895 0.04% 83 10716 0.04% 84 13508 0.06% 85 16333 0.07% 86 16862 0.07% 87 18221 0.07% 88 20428 0.08% 89 20278 0.08% 90 22522 0.09% 91 23255 0.09% 92 27837 0.11% 93 27978 0.11% 94 30093 0.12% 95 33495 0.14% 96 31499 0.13% 97 32668 0.13% 98 36239 0.15% 99 36937 0.15% 100 38069 0.16% 101 39174 0.16% 102 43635 0.18% 103 43051 0.18% 104 48981 0.20% 105 51595 0.21% 106 50693 0.21% 107 52685 0.22% 108 53586 0.22% 109 53424 0.22% 110 56179 0.23% 111 59966 0.24% 112 61186 0.25% 113 62286 0.25% 114 70965 0.29% 115 76173 0.31% 116 78299 0.32% 117 78694 0.32% 118 74811 0.31% 119 73714 0.30% 120 73712 0.30% 121 78102 0.32% 122 91998 0.38% 123 89635 0.37% 124 93998 0.38% 125 100052 0.41% 126 89566 0.37% 127 96416 0.39% 128 96594 0.39% 129 101576 0.41% 130 91473 0.37% 131 100661 0.41% 132 107503 0.44% 133 101445 0.41% 134 113613 0.46% 135 108246 0.44% 136 110849 0.45% 137 110774 0.45% 138 120699 0.49% 139 124569 0.51% 140 124728 0.51% 141 148072 0.60% 142 136714 0.56% 143 150388 0.61% 144 160367 0.65% 145 188930 0.77% 146 203313 0.83% 147 242080 0.99% 148 342132 1.40% 149 608936 2.49% 150 4032071 16.46% 151 14491776 59.15% 24499888 reads passed initial QC criterion=sequence-density sequence-density=0.38 sequence-density-rank=1 fanout-score=5.43 fanout-score-rank=13 prefix-density=1.20 prefix-fanout=1.7 sequence=CAGCCTCACGCGGTGCCTGCCGCTCTAGGATCCGTGAGGCCCAGCTTTCGCATAGGCCCCAGCAGATCCACTACGCAA criterion=fanout-score sequence-density=0.01 sequence-density-rank=44 fanout-score=63.74 fanout-score-rank=1 prefix-density=0.10 prefix-fanout=3.8 sequence=CTTTTTATTTTGTTTCTTTTTATTTAGACCTTCTTCATATTTAGTTTTATCTATTAATCGATAGTATCTACCGGCGCGAACTCGAATTTGATCGCCTTCCATACTTCACAAGCTGCGGCTAGTTCAGGACTCCATTTGCAAGCTGCTCGGATAATTTCATTACCTTCACGAGCAAGATCGCGCCCTTCGTTACGAGCTTGTACACAGGCTTCTAAAGCCACTCGATTAGCTGCTGCACCAGGTGCATTTCCCCAAGGATGTCCTAAAGTTCCTCCACCAAATTGTAATACAGAATCATCCCCAAAGATTTCGGTCAGAGCTGGCATATGCCAAACATGAATACCACCTGAAGCTACTGGTATAACACCTGGCATGGATACCCAGTCCTGAGTGAAAAAGATACCGCGAGCACGATCTTTTTCAATAAAATCGTCGCGCAATAAATCAACAAAACCTAAAGTGATTTCGCGTTCCCCTTCTAACTTACCTACTACTGTACCGGCGTGGATAT criterion=sequence-density sequence-density=0.41 sequence-density-rank=1 fanout-score=3.04 fanout-score-rank=16 prefix-density=1.24 prefix-fanout=1.0 sequence=TTGCGTAGTGGATCTGCTGGGGCCTATGCGAAAGCTGGGCCTCACGGATCCTAGAGCGGCAGGCACCGCGTGAGGCTG criterion=fanout-score sequence-density=0.01 sequence-density-rank=36 fanout-score=41.97 fanout-score-rank=1 prefix-density=0.05 prefix-fanout=7.5 sequence=TTTTTTTTTATGAGATTTTTGCTAAAGTTTCATTTACGCCTAATTCACATCGAGTAGACCTTGTTATTGTGAGAATTCTTAATTCAAGAGTTGTAAGGAGGGACTTATGTCACCACAAACAGAAACTAAAGCAAGTGTTGGATTTAAAGCTGGTGTTAAAGATTATAGATTGACTTACTACACCCCGGAGTATGAAACCAAGGATACTGATATCTTGGCAGCATTCCGAGTATCTCCTCAACCTGGGGTTCCGCCCGAAGAAGCAGGGGCTGCAGTAGCTGCCGAATCTTCTACTGGTACATGGACAACTGTTTGGACTGATGGACTTACTAGTCTTGATCGTTACAAAGGACGATGCTATCACATCGAGCCTGTTCCTGGGGAAGACAGTCAATGGATCTGTTATGTAGCTTATCCATTAGATCTATTTGAAGAGGGTTCCGTTACTAACATGTTTACTTCCATTGTAGGTAACGTATTTGGTTTCAAAGCCCTACGTGCTCTACGTCTG SRR6941591 testing PE reads STAR mapping to Ensembl genome Started job on | Dec 06 12:24:28 Started mapping on | Dec 06 12:24:28 Finished on | Dec 06 12:26:53 Mapping speed, Million of reads per hour | 608.27 Number of input reads | 24499888 Average input read length | 291 UNIQUE READS: Uniquely mapped reads number | 15930094 Uniquely mapped reads % | 65.02% Average mapped length | 293.17 Number of splices: Total | 2934136 Number of splices: Annotated (sjdb) | 2638229 Number of splices: GT/AG | 2780260 Number of splices: GC/AG | 35321 Number of splices: AT/AC | 11517 Number of splices: Non-canonical | 107038 Mismatch rate per base, % | 0.30% Deletion rate per base | 0.02% Deletion average length | 1.89 Insertion rate per base | 0.02% Insertion average length | 2.35 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 7346494 % of reads mapped to multiple loci | 29.99% Number of reads mapped to too many loci | 39209 % of reads mapped to too many loci | 0.16% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 3.81% % of reads unmapped: other | 1.03% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 1241852 1241852 1241852 N_multimapping 7346494 7346494 7346494 N_noFeature 6537580 15413832 6765399 N_ambiguous 521790 5848 239412 UnstrandedReadsAssigned:8870724 PositiveStrandReadsAssigned:510414 NegativeStrandReadsAssigned:8925283 Dataset is classified negative stranded MeadianReadLen=151 20thPercentileLength=149 echo kmer=145 SRR6941591 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,972 [index] number of k-mers: 66,720,672 [index] number of equivalence classes: 111,837 [quant] running in paired-end mode [quant] will process pair 1: SRR6941591-trimmed-pair1.fastq SRR6941591-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 24,499,888 reads, 13,497,739 reads pseudoaligned [quant] estimated average fragment length: 217.067 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,019 rounds 52973 SRR6941591.ke.tsv 35125 SRR6941591.se.tsv 88098 total ==> SRR6941591.ke.tsv <== target_id length eff_length est_counts tpm PNS24245 936 720.415 0 0 PNS24247 1044 827.933 11.6921 1.05984 PNS24249 1928 1711.93 23.0985 1.01261 PNS24246 1044 827.933 11.6921 1.05984 PNS24248 1044 827.933 11.6921 1.05984 PNS24244 1471 1254.93 41.8251 2.50126 PNS24243 293 112.891 0 0 KQK14069 1603 1386.93 2787.66 150.844 KQK14071 474 268.074 59.4573 16.6454 ==> SRR6941591.se.tsv <== BRADI_1g14170v3 3656 BRADI_1g53295v3 59 BRADI_1g59795v3 99 BRADI_1g07683v3 0 BRADI_1g00485v3 0 BRADI_1g20270v3 59 BRADI_1g74790v3 8 BRADI_1g09890v3 0 BRADI_1g77505v3 87 BRADI_1g48960v3 0 SRR6941591 completed mapping pipeline successfully