Starting /dee2/code/volunteer_pipeline.sh SRR6941592 current disk space = 1550850998272 free memory = 1602338296 SRR6941592 SRAfilesize 413ed4e3de4ec684171f1aee7d835c9f SRR6941592.sra SRR6941592.sra file validated SRR6941592 is paired end SRR6941592 is conventional basespace SRR6941592 read1 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR6941592_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.745 33.0 33.0 34.0 32.0 34.0 2 33.05275 34.0 33.0 34.0 32.0 34.0 3 33.19675 34.0 33.0 34.0 32.0 34.0 4 33.3665 34.0 33.0 34.0 33.0 34.0 5 33.3825 34.0 33.0 34.0 33.0 34.0 6 37.13125 38.0 38.0 38.0 36.0 38.0 7 37.54975 38.0 38.0 38.0 37.0 38.0 8 37.58275 38.0 38.0 38.0 38.0 38.0 9 37.63725 38.0 38.0 38.0 38.0 38.0 10-14 37.664249999999996 38.0 38.0 38.0 38.0 38.0 15-19 37.6633 38.0 38.0 38.0 38.0 38.0 20-24 37.64815 38.0 38.0 38.0 38.0 38.0 25-29 37.61424999999999 38.0 38.0 38.0 38.0 38.0 30-34 37.5139 38.0 38.0 38.0 38.0 38.0 35-39 37.5522 38.0 38.0 38.0 38.0 38.0 40-44 37.5236 38.0 38.0 38.0 38.0 38.0 45-49 37.528800000000004 38.0 38.0 38.0 38.0 38.0 50-54 37.52635 38.0 38.0 38.0 38.0 38.0 55-59 37.49065 38.0 38.0 38.0 38.0 38.0 60-64 37.4788 38.0 38.0 38.0 37.6 38.0 65-69 37.3346 38.0 38.0 38.0 37.0 38.0 70-74 37.28245 38.0 38.0 38.0 37.0 38.0 75-79 37.3909 38.0 38.0 38.0 37.2 38.0 80-84 37.3414 38.0 38.0 38.0 37.0 38.0 85-89 37.12885 38.0 38.0 38.0 36.4 38.0 90-94 37.14975 38.0 38.0 38.0 36.4 38.0 95-99 37.0129 38.0 38.0 38.0 36.0 38.0 100-104 37.0641 38.0 38.0 38.0 36.0 38.0 105-109 36.936099999999996 38.0 38.0 38.0 35.2 38.0 110-114 36.543499999999995 38.0 38.0 38.0 34.6 38.0 115-119 36.431 38.0 38.0 38.0 34.2 38.0 120-124 36.4711 38.0 38.0 38.0 34.0 38.0 125-129 36.499900000000004 38.0 38.0 38.0 34.2 38.0 130-134 36.4525 38.0 38.0 38.0 34.0 38.0 135-139 36.19279999999999 38.0 38.0 38.0 33.2 38.0 140-144 35.89745 38.0 36.8 38.0 32.8 38.0 145-149 35.535199999999996 38.0 36.0 38.0 31.2 38.0 150-151 31.597 35.5 32.0 38.0 16.5 38.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 5 1.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 2.0 15 1.0 16 1.0 17 1.0 18 0.0 19 1.0 20 2.0 21 0.0 22 1.0 23 4.0 24 6.0 25 8.0 26 11.0 27 6.0 28 17.0 29 17.0 30 34.0 31 27.0 32 41.0 33 74.0 34 107.0 35 167.0 36 429.0 37 3042.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 36.55100443516828 10.957474563005478 8.635533524654319 43.85598747717193 2 24.281070267566893 13.953488372093023 33.383345836459114 28.382095523880967 3 20.325 20.225 25.1 34.35 4 24.7 27.6 21.775 25.924999999999997 5 24.5 31.7 22.35 21.45 6 20.3 33.1 24.45 22.15 7 16.875 21.775 40.425 20.925 8 19.25 22.3 30.099999999999998 28.349999999999998 9 18.625 19.900000000000002 33.550000000000004 27.925 10-14 22.205 26.915 24.15 26.729999999999997 15-19 22.43 25.105 26.52 25.945 20-24 22.49 25.724999999999998 25.965 25.82 25-29 23.106155307765388 24.65123256162808 26.296314815740786 25.946297314865742 30-34 22.365 25.85 25.385 26.400000000000002 35-39 22.137213721372138 25.562556255625562 26.302630263026305 25.997599759975998 40-44 22.368947579031612 25.060024009603843 26.355542216886757 26.215486194477787 45-49 20.925 25.15 27.455000000000002 26.47 50-54 22.288915566226493 24.9749899959984 25.755302120848338 26.98079231692677 55-59 21.673250987648146 25.36880532079812 25.86888033204981 27.089063359503925 60-64 21.76217621762176 25.212521252125214 26.242624262426244 26.782678267826782 65-69 22.805 25.15 25.405 26.640000000000004 70-74 23.24732473247325 25.597559755975595 24.69246924692469 26.46264626462646 75-79 22.43 25.31 25.224999999999998 27.034999999999997 80-84 23.512351235123514 25.437543754375437 25.092509250925094 25.95759575957596 85-89 22.80114005700285 25.391269563478176 25.686284314215712 26.121306065303262 90-94 22.047204720472045 26.1976197619762 24.59245924592459 27.16271627162716 95-99 21.82 25.66 25.235000000000003 27.284999999999997 100-104 22.262791977192016 26.064122442855 24.643625268844097 27.02946031110889 105-109 22.785 25.7 25.814999999999998 25.7 110-114 22.47439244828279 25.999196625828482 25.808395260092386 25.718015665796344 115-119 22.10468264313647 26.36117517296701 24.872154818008624 26.661987365887896 120-124 22.613744431653235 27.013364032233845 23.179338305220483 27.193553230892437 125-129 22.894578915783157 26.200240048009604 24.00480096019204 26.9003800760152 130-134 23.01230123012301 26.137613761376137 23.737373737373737 27.11271127112711 135-139 22.715 26.735 24.57 25.979999999999997 140-144 23.119999999999997 26.640000000000004 23.59 26.650000000000002 145-149 22.35 26.584999999999997 24.16 26.905 150-151 22.112499999999997 26.137500000000003 24.6 27.150000000000002 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 1.5 20 2.0 21 2.0 22 4.0 23 3.5 24 1.5 25 2.5 26 6.0 27 8.5 28 11.0 29 15.0 30 18.5 31 18.0 32 19.5 33 22.0 34 23.5 35 33.0 36 63.5 37 123.5 38 130.5 39 106.0 40 127.0 41 154.0 42 141.0 43 138.5 44 144.5 45 132.5 46 110.0 47 88.5 48 90.0 49 89.5 50 114.5 51 126.5 52 126.5 53 139.0 54 175.5 55 252.5 56 264.0 57 214.0 58 188.5 59 151.5 60 113.5 61 87.0 62 52.5 63 39.5 64 29.0 65 16.5 66 13.0 67 12.0 68 11.5 69 6.0 70 5.0 71 6.0 72 6.0 73 4.5 74 3.0 75 4.0 76 3.0 77 2.0 78 2.0 79 0.5 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 4.175 2 0.025 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.005 30-34 0.0 35-39 0.01 40-44 0.04 45-49 0.0 50-54 0.04 55-59 0.015 60-64 0.01 65-69 0.0 70-74 0.01 75-79 0.0 80-84 0.01 85-89 0.005 90-94 0.01 95-99 0.0 100-104 0.034999999999999996 105-109 0.0 110-114 0.42 115-119 0.27 120-124 0.105 125-129 0.02 130-134 0.01 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 71.05 #Duplication Level Percentage of deduplicated Percentage of total 1 81.4567206192822 57.875 2 9.781843771991555 13.900000000000002 3 3.764954257565095 8.025 4 2.322308233638283 6.6000000000000005 5 0.9148486980999296 3.25 6 0.633356790992259 2.7 7 0.38705137227304715 1.925 8 0.211118930330753 1.2 9 0.17593244194229415 1.125 >10 0.3518648838845883 3.4000000000000004 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCATTAAT 28 0.7000000000000001 No Hit GTCGGTTTCGGGTACAGGTACCCTTTTGTTGAAGGTCGTTCGAGCTTTTC 14 0.35000000000000003 No Hit TTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCATTAATA 14 0.35000000000000003 No Hit GTACAAGGCCCGGGAACGGATTCACCGCCGTATGGCTGACCGGCGATTAC 13 0.325 No Hit GGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTACGTT 13 0.325 No Hit CCACATGCTCCACCGCTTGTGCGGGCCCCCGTCAATTCCTTTGAGTTTCA 11 0.27499999999999997 No Hit GCGGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTACG 11 0.27499999999999997 No Hit CTCATAAGGACCACCATTGTATAACCATTCATCAACGGATGCAGCTTCCC 11 0.27499999999999997 No Hit GTTGAAACTAGCATATTGGAAGATTAATCGGCCAAAATAACCATGAGCAG 11 0.27499999999999997 No Hit GGCCAAAATAACCATGAGCAGCCACAATATTATAAGTCTCTTCCTCTTGA 10 0.25 No Hit CACCGCTTGTGCGGGCCCCCGTCAATTCCTTTGAGTTTCATTCTTGCGAA 9 0.22499999999999998 No Hit GTCGCCCAGGGCATAAGGGGCATGATGACTTGGCCTCATCCTCTCCTTCC 9 0.22499999999999998 No Hit CTCAGATACCGTCATTGTTTCTTCTCCGAGAAAAGAAGTTGACGACCCGT 9 0.22499999999999998 No Hit GCCAAAATAACCATGAGCAGCCACAATATTATAAGTCTCTTCCTCTTGAC 9 0.22499999999999998 No Hit CTTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCAT 9 0.22499999999999998 No Hit GGCATGATGACTTGGCCTCATCCTCTCCTTCCTCCGGCTTAACACCGGCG 8 0.2 No Hit GCTTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCA 8 0.2 No Hit CTTTGTCCCGCCCATTGTAGCACGTGTGTCGCCCAGGGCATAAGGGGCAT 8 0.2 No Hit CTCATTAGCAGATTCATTTTCAGTAGTTTCCCTGATCAAACTAGAGGTTA 8 0.2 No Hit CTCCACGCGGCATTGCTCCGTCAGGCTTTCGCCCATTGCGGAAAATTCCC 8 0.2 No Hit GTTTACGGCTAGGACTACTGGGGTCTCTAATCCCATTTGCTCCCCTAGCT 8 0.2 No Hit GCCGAAAACACCAGCTACACCTAACATGTGAAATGGATGCATAAGGATGT 7 0.17500000000000002 No Hit TTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCATT 7 0.17500000000000002 No Hit CTCATCTTGGGGTGGGCTTACTACTTATATGCTTTCAGCAGTTATCCTCT 7 0.17500000000000002 No Hit TTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCATTA 7 0.17500000000000002 No Hit GGATGCATAAGGATGTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAA 7 0.17500000000000002 No Hit GCTAGTTATCCAGTTGCAGAAGCGACCCCACAGGCTTGTACTTTCGCGTC 7 0.17500000000000002 No Hit ATATAACAAGCTACACCAAGTAAGAAGTGTAGAACAATTAGCTCATAAGG 7 0.17500000000000002 No Hit CACCATTGTATAACCATTCATCAACGGATGCAGCTTCCCAAATTGGGTAA 7 0.17500000000000002 No Hit GCGGAAAAACCTTATCCATTAATAGCGGGAACTTCAAGAGCAGCTAGATC 7 0.17500000000000002 No Hit CCGGGAACGGATTCACCGCCGTATGGCTGACCGGCGATTACTAGCGATTC 7 0.17500000000000002 No Hit CTTTCTTTTCCTCTGGCTACTAAGATGTTTCAGTTCGCCAGGTTGTCTCT 7 0.17500000000000002 No Hit CCCGAAGTTACGGGGCTATTTTGCCGAGTTCCTTAGAGAGAGTTGTCTCG 6 0.15 No Hit CTCCACCGCTTGTGCGGGCCCCCGTCAATTCCTTTGAGTTTCATTCTTGC 6 0.15 No Hit GGGCGGTGTGTACAAGGCCCGGGAACGGATTCACCGCCGTATGGCTGACC 6 0.15 No Hit CGTCAATTCCTTTGAGTTTCATTCTTGCGAACGTACTCCCCAGGCGGGAT 6 0.15 No Hit CAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCATTAATAGCGGGAA 6 0.15 No Hit ACCGCTTGTGCGGGCCCCCGTCAATTCCTTTGAGTTTCATTCTTGCGAAC 6 0.15 No Hit GCTCCACCGCTTGTGCGGGCCCCCGTCAATTCCTTTGAGTTTCATTCTTG 6 0.15 No Hit CCAGATTCCTACTACAGGCCAAGCAGCCAAGAAGAAGTGTAAAGAACGAG 6 0.15 No Hit CGGGAACGGATTCACCGCCGTATGGCTGACCGGCGATTACTAGCGATTCC 6 0.15 No Hit GCTGCGATGAAGGCGATAATAAATACAGAAGTTGCGGTCAATAAGGTAGG 6 0.15 No Hit GGAAGTTGTGAGCATTACGTTCGTGCATTACTTCCATACCAAGATTAGCA 6 0.15 No Hit GGAACGGATTCACCGCCGTATGGCTGACCGGCGATTACTAGCGATTCCTG 6 0.15 No Hit GCGGTGTGTACAAGGCCCGGGAACGGATTCACCGCCGTATGGCTGACCGG 6 0.15 No Hit AGCGGAAAAACCTTATCCATTAATAGCGGGAACTTCAAGAGCAGCTAGAT 6 0.15 No Hit GTGCGACGTGGGGCTGGATCTCAGTGGATCGTGGCAGCAAGGCCACTCTG 6 0.15 No Hit GGCAATTTCAAGCACTCTTTGACTCTCTTTTCAAAGTCCTTTTCATCTTT 6 0.15 No Hit CCACCGCTTGTGCGGGCCCCCGTCAATTCCTTTGAGTTTCATTCTTGCGA 6 0.15 No Hit CCCCACTGCTGCCTCCCGTAGGAGTCTGGGCCGTGTCTCAGTCCCAGTGT 6 0.15 No Hit GCCCACACCGGATATGGACCGAACTGTCTCACGACGTTCTGAACCCAGCT 5 0.125 No Hit CTAGTATCCATCGTTTACGGCTAGGACTACTGGGGTCTCTAATCCCATTT 5 0.125 No Hit GCGGTCAATAAGGTAGGGATCATCAAAACACCGAACCATCCGATGTAAAG 5 0.125 No Hit CGCAGAAGTAGGAATAATAGCACCAGAGATAATATTGTTTCCATAAAGTA 5 0.125 No Hit AGATAATATTGTTTCCATAAAGTAAAGAACCAGAAACAGGCTCGCGAATA 5 0.125 No Hit CTGCGATGAAGGCGATAATAAATACAGAAGTTGCGGTCAATAAGGTAGGG 5 0.125 No Hit CAGATATTCCTAAAGGCATACCATCAGAGAAGCTTCCTTGACCAATAGGG 5 0.125 No Hit GTACAAAGGGCAGGGACGTAGTCAACGCGAGCTGATGACTCGCGCTTACT 5 0.125 No Hit GTCAGGCTTTCGCCCATTGCGGAAAATTCCCCACTGCTGCCTCCCGTAGG 5 0.125 No Hit GTGTACAAGGCCCGGGAACGGATTCACCGCCGTATGGCTGACCGGCGATT 5 0.125 No Hit CGGGCGGTGTGTACAAGGCCCGGGAACGGATTCACCGCCGTATGGCTGAC 5 0.125 No Hit CATAGTACTAATACCTAAAGCAGTGAACCAGATTCCTACTACAGGCCAAG 5 0.125 No Hit GTCAATTCCTTTGAGTTTCATTCTTGCGAACGTACTCCCCAGGCGGGATA 5 0.125 No Hit ACATGCTCCACCGCTTGTGCGGGCCCCCGTCAATTCCTTTGAGTTTCATT 5 0.125 No Hit GCCACCTACAGACGCTTTACGCCCAATCATTCCGGATAACGCTTGCATCC 5 0.125 No Hit GGTCAATAAGGTAGGGATCATCAAAACACCGAACCATCCGATGTAAAGAC 5 0.125 No Hit CCGAAGTTACGGGGCTATTTTGCCGAGTTCCTTAGAGAGAGTTGTCTCGC 5 0.125 No Hit CCCTACCGTACTCCAGCTTGGTAGTTTCCACCGCCTGTCCAGGGTTGAGC 5 0.125 No Hit GCCCTATGAAGACTCGCTTTCGCTACGGCTCCGGTGGGTTCCGTTCCCTT 5 0.125 No Hit CTCAGTGTCAGTGTCGGCCCAGCAGAGTGCTTTCGCCGTTGGTGTTCTTT 5 0.125 No Hit CCTTAACCAAGCCACTGCCTATGAGTCGCCGGCTCATTCTTCAACAGGCA 5 0.125 No Hit CTTGATTTCACCGGTTTCCGCCTGTGATTTATAAATAGCTTCGGCACAAA 5 0.125 No Hit GCTAGATCTAGAGGGAAGTTGTGAGCATTACGTTCGTGCATTACTTCCAT 5 0.125 No Hit CTCTTGACCAAATTTGTAACCCTCATTAGCAGATTCATTTTCAGTAGTTT 5 0.125 No Hit GCCTCATCCTCTCCTTCCTCCGGCTTAACACCGGCGGTCTGTTCAGGGTT 5 0.125 No Hit GCCAAGAAGAAGTGTAAAGAACGAGAGTTGTTGAAACTAGCATATTGGAA 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.025 0.0 0.0 0.0 0.0 70-71 0.025 0.0 0.0 0.0 0.0 72-73 0.075 0.0 0.0 0.0 0.0 74-75 0.1 0.0 0.0 0.0 0.0 76-77 0.1375 0.0 0.0 0.0 0.0 78-79 0.15 0.0 0.0 0.0 0.0 80-81 0.175 0.0 0.0 0.0 0.0 82-83 0.2375 0.0 0.0 0.0 0.0 84-85 0.3125 0.0 0.0 0.0 0.0 86-87 0.35 0.0 0.0 0.0 0.0 88-89 0.4125 0.0 0.0 0.0 0.0 90-91 0.55 0.0 0.0 0.0 0.0 92-93 0.6625 0.0 0.0 0.0 0.0 94-95 0.875 0.0 0.0 0.0 0.0 96-97 1.125 0.0 0.0 0.0 0.0 98-99 1.3875 0.0 0.0 0.0 0.0 100-101 1.725 0.0 0.0 0.0 0.0 102-103 2.1375 0.0 0.0 0.0 0.0 104-105 2.5875 0.0 0.0 0.0 0.0 106-107 3.05 0.0 0.0 0.0 0.0 108-109 3.375 0.0 0.0 0.0 0.0 110-111 4.0375 0.0 0.0 0.0 0.0 112-113 4.525 0.0 0.0 0.0 0.0 114-115 5.0875 0.0 0.0 0.0 0.0 116-117 5.737500000000001 0.0 0.0 0.0 0.0 118-119 6.449999999999999 0.0 0.0 0.0 0.0 120-121 7.275 0.0 0.0 0.0 0.0 122-123 7.8375 0.0 0.0 0.0 0.0 124-125 8.725 0.0 0.0 0.0 0.0 126-127 9.5125 0.0 0.0 0.0 0.0 128-129 10.4375 0.0 0.0 0.0 0.0 130-131 11.225 0.0 0.0 0.0 0.0 132-133 12.05 0.0 0.0 0.0 0.0 134-135 13.05 0.0 0.0 0.0 0.0 136-137 13.9625 0.0 0.0 0.0 0.0 138-139 14.9625 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CACCTGT 10 0.0068555363 144.825 8 >>END_MODULE SRR6941592 read2 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR6941592_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 33.24325 34.0 33.0 34.0 33.0 34.0 2 33.33075 34.0 33.0 34.0 33.0 34.0 3 33.33675 34.0 33.0 34.0 33.0 34.0 4 33.358 34.0 33.0 34.0 33.0 34.0 5 33.31425 34.0 33.0 34.0 33.0 34.0 6 37.5085 38.0 38.0 38.0 38.0 38.0 7 37.595 38.0 38.0 38.0 38.0 38.0 8 37.41225 38.0 38.0 38.0 38.0 38.0 9 37.49925 38.0 38.0 38.0 38.0 38.0 10-14 37.443850000000005 38.0 38.0 38.0 38.0 38.0 15-19 37.43205 38.0 38.0 38.0 38.0 38.0 20-24 37.44855 38.0 38.0 38.0 38.0 38.0 25-29 37.3934 38.0 38.0 38.0 37.6 38.0 30-34 37.4184 38.0 38.0 38.0 38.0 38.0 35-39 37.446299999999994 38.0 38.0 38.0 38.0 38.0 40-44 37.4125 38.0 38.0 38.0 38.0 38.0 45-49 37.4139 38.0 38.0 38.0 38.0 38.0 50-54 37.3994 38.0 38.0 38.0 37.8 38.0 55-59 37.33995 38.0 38.0 38.0 37.2 38.0 60-64 37.335249999999995 38.0 38.0 38.0 37.0 38.0 65-69 37.2881 38.0 38.0 38.0 37.0 38.0 70-74 37.305 38.0 38.0 38.0 37.0 38.0 75-79 37.24185000000001 38.0 38.0 38.0 37.0 38.0 80-84 37.0923 38.0 38.0 38.0 36.6 38.0 85-89 37.05329999999999 38.0 38.0 38.0 36.4 38.0 90-94 37.0437 38.0 38.0 38.0 36.0 38.0 95-99 37.030350000000006 38.0 38.0 38.0 35.8 38.0 100-104 36.6531 38.0 38.0 38.0 35.0 38.0 105-109 36.56745 38.0 38.0 38.0 34.6 38.0 110-114 36.1732 38.0 38.0 38.0 33.6 38.0 115-119 35.995000000000005 38.0 37.8 38.0 33.0 38.0 120-124 35.9683 38.0 37.8 38.0 32.8 38.0 125-129 35.9857 38.0 38.0 38.0 33.0 38.0 130-134 36.03675 38.0 38.0 38.0 33.2 38.0 135-139 35.728699999999996 38.0 36.8 38.0 31.4 38.0 140-144 35.20005 38.0 36.0 38.0 31.0 38.0 145-149 34.196799999999996 38.0 34.8 38.0 26.4 38.0 150-151 29.991500000000002 35.5 27.0 38.0 11.5 38.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 1.0 4 1.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 1.0 12 0.0 13 2.0 14 0.0 15 1.0 16 1.0 17 6.0 18 1.0 19 3.0 20 8.0 21 0.0 22 3.0 23 9.0 24 8.0 25 11.0 26 13.0 27 10.0 28 23.0 29 23.0 30 35.0 31 42.0 32 50.0 33 80.0 34 112.0 35 196.0 36 459.0 37 2899.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 41.225 16.25 12.4 30.125 2 31.25 17.849999999999998 31.624999999999996 19.275000000000002 3 23.1 22.7 32.125 22.075 4 27.375 30.95 23.275000000000002 18.4 5 28.999999999999996 31.775 20.974999999999998 18.25 6 24.2 34.675 21.575 19.55 7 20.849999999999998 19.275000000000002 38.125 21.75 8 24.625 21.9 26.875 26.6 9 25.775 22.3 27.425 24.5 10-14 27.76 24.665 24.98 22.595000000000002 15-19 27.435 25.055 25.525 21.985 20-24 27.544999999999998 25.580000000000002 25.419999999999998 21.455 25-29 27.512751275127513 25.332533253325334 25.24252425242524 21.91219121912191 30-34 27.251362568128407 25.66628331416571 24.90624531226561 22.176108805440272 35-39 26.2976297629763 26.33763376337634 25.397539753975394 21.967196719671968 40-44 26.540000000000003 26.400000000000002 24.875 22.185 45-49 26.776338816940846 26.146307315365767 25.02125106255313 22.056102805140256 50-54 27.66638331916596 25.006250312515625 25.141257062853146 22.186109305465273 55-59 26.584999999999997 26.179999999999996 24.59 22.645 60-64 27.061353067653382 25.36626831341567 25.796289814490724 21.77608880444022 65-69 27.05905885882882 25.47882182327349 25.2137820673101 22.24833725058759 70-74 27.285457091418287 24.65493098619724 25.76015203040608 22.299459891978398 75-79 27.336366818340917 24.636231811590577 25.631281564078208 22.3961198059903 80-84 27.437346806062727 25.451453153919264 25.641538692411586 21.469661347606422 85-89 27.889183377506626 25.558833825073762 24.828724308646297 21.723258488773318 90-94 27.321366068303416 25.616280814040703 25.216260813040652 21.84609230461523 95-99 27.68 25.695 24.845 21.78 100-104 27.426856714178545 26.066516629157288 25.211302825706426 21.295323830957738 105-109 28.87743356188379 24.223011861268205 25.504229017566686 21.395325559281318 110-114 27.076353817690883 26.051302565128253 24.841242062103106 22.031101555077754 115-119 27.91139556977849 25.786289314465723 24.831241562078105 21.471073553677684 120-124 27.405 26.840000000000003 23.78 21.975 125-129 27.986399319965997 26.66133306665333 23.251162558127906 22.101105055252763 130-134 28.432108027006752 25.641410352588146 24.111027756939237 21.815453863465866 135-139 27.561378068903448 26.121306065303262 24.891244562228113 21.426071303565177 140-144 28.675735147029407 25.575115023004603 24.889977995599118 20.859171834366876 145-149 28.68147258903561 25.77531012404962 25.06502601040416 20.478191276510604 150-151 29.19169169169169 24.874874874874877 23.973973973973976 21.95945945945946 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 1.5 22 2.0 23 0.5 24 2.5 25 4.5 26 4.5 27 9.5 28 15.0 29 20.0 30 17.5 31 14.5 32 17.5 33 23.5 34 40.0 35 54.0 36 64.5 37 88.5 38 104.0 39 115.5 40 136.5 41 129.0 42 126.5 43 140.5 44 147.5 45 133.0 46 104.5 47 99.5 48 99.5 49 99.0 50 107.5 51 121.5 52 112.0 53 131.0 54 205.0 55 259.0 56 240.0 57 175.5 58 154.5 59 152.5 60 127.0 61 106.0 62 81.0 63 51.0 64 32.0 65 21.5 66 17.0 67 15.5 68 16.0 69 12.0 70 9.5 71 8.0 72 6.5 73 7.0 74 5.0 75 2.5 76 3.0 77 3.0 78 2.0 79 1.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.01 30-34 0.005 35-39 0.01 40-44 0.0 45-49 0.005 50-54 0.005 55-59 0.0 60-64 0.005 65-69 0.015 70-74 0.02 75-79 0.005 80-84 0.045 85-89 0.015 90-94 0.005 95-99 0.0 100-104 0.025 105-109 0.095 110-114 0.005 115-119 0.005 120-124 0.0 125-129 0.005 130-134 0.025 135-139 0.005 140-144 0.02 145-149 0.04 150-151 0.1 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 71.05 #Duplication Level Percentage of deduplicated Percentage of total 1 80.2603800140746 57.025000000000006 2 11.048557353976072 15.7 3 4.433497536945813 9.45 4 1.9000703729767767 5.4 5 0.9148486980999296 3.25 6 0.7037297677691766 3.0 7 0.211118930330753 1.05 8 0.1055594651653765 0.6 9 0.07037297677691766 0.44999999999999996 >10 0.3518648838845883 4.075 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTTCGATCCTGGCTCAGGATGAACGCTGGCGGCATGCTTAACACATGCAA 40 1.0 No Hit ATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAA 24 0.6 No Hit GGCTTGCGGTGGATACCTAGGCACCCAGAGACGAGGAAGGGCGTAGCAAG 18 0.44999999999999996 No Hit CCTTACCAGGGCTTGACATGCCGCGAATCCTCTTGAAAGAGAGGGGTGCC 15 0.375 No Hit CTTACCAGGGCTTGACATGCCGCGAATCCTCTTGAAAGAGAGGGGTGCCC 14 0.35000000000000003 No Hit GAACCTTACCAGGGCTTGACATGCCGCGAATCCTCTTGAAAGAGAGGGGT 11 0.27499999999999997 No Hit GCTTGTTATATGGGTCGTGAGTGGGAACTTAGTTTCCGTTTGGGTATGCG 11 0.27499999999999997 No Hit CCTGGCTCAGGATGAACGCTGGCGGCATGCTTAACACATGCAAGTCGAAC 10 0.25 No Hit GTGACCAGGCCCGGGCGACTGTTTACCAAAAACACAGGTCTCCGCAAAGT 10 0.25 No Hit AGTGACCAGGCCCGGGCGACTGTTTACCAAAAACACAGGTCTCCGCAAAG 10 0.25 No Hit GGTCGCTTCTGCAACTGGATAACTAGCACTGAAAATCGTCTTTACATCGG 9 0.22499999999999998 No Hit ACCTTACCAGGGCTTGACATGCCGCGAATCCTCTTGAAAGAGAGGGGTGC 9 0.22499999999999998 No Hit CTATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAA 8 0.2 No Hit ATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCA 8 0.2 No Hit GGATGATCAGCCACACTGGGACTGAGACACGGCCCAGACTCCTACGGGAG 8 0.2 No Hit GGAATTTCCGGTGGAGCGGTGAAATGCATTGAGATCGGAAAGAACACCAA 7 0.17500000000000002 No Hit TTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAA 7 0.17500000000000002 No Hit ATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCTAAATGG 7 0.17500000000000002 No Hit CTAGCACTGAAAATCGTCTTTACATCGGATGGTTCGGTGTTTTGATGATC 7 0.17500000000000002 No Hit GTTGGGTTAAGTCTCGCAACGAGCGCAACCCTCGTGTTTAGTTGCCACTA 7 0.17500000000000002 No Hit GGTACTTTCAACTTTATGATTGTATTCCAGGCAGAGCACAACATCCTTAT 7 0.17500000000000002 No Hit GTTAAGTCTCGCAACGAGCGCAACCCTCGTGTTTAGTTGCCACTATGAGT 6 0.15 No Hit TGGGAACTTAGTTTCCGTTTGGGTATGCGCCCTTGGATTGCTGTTGCATA 6 0.15 No Hit GTTCTGGGCCGCACGCGCGCTACACTGATGTATTCAACGAGTATATAGCC 6 0.15 No Hit AGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGATCT 6 0.15 No Hit CCTGCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGCAA 6 0.15 No Hit CTTGTTATATGGGTCGTGAGTGGGAACTTAGTTTCCGTTTGGGTATGCGC 6 0.15 No Hit CTCGGGAACGCGGACACAGGTGGTGCATGGCTGTCGTCAGCTCGTGCCGT 6 0.15 No Hit CGGACATTGGTCCTCGAGTGCAAAGGCAGAAGGGAGCTTGACTGCAAGAC 6 0.15 No Hit GCTTGACATGCCGCGAATCCTCTTGAAAGAGAGGGGTGCCCTCGGGAACG 6 0.15 No Hit GGAAGCTTCTCTGATGGTATGCCTTTAGGAATATCTGGTACTTTCAACTT 6 0.15 No Hit GTGGGAACTTAGTTTCCGTTTGGGTATGCGCCCTTGGATTGCTGTTGCAT 6 0.15 No Hit ATTAGAGACCCCAGTAGTCCTAGCCGTAAACGATGGATACTAGGTGCTGT 6 0.15 No Hit GTTTCAACAACTCTCGTTCTTTACACTTCTTCTTGGCTGCTTGGCCTGTA 6 0.15 No Hit TCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGA 6 0.15 No Hit GCTGCGACTGCTGTTTTCTTGATTTACCCTATTGGTCAAGGAAGCTTCTC 6 0.15 No Hit CGCGAATCCTCTTGAAAGAGAGGGGTGCCCTCGGGAACGCGGACACAGGT 6 0.15 No Hit GCAAAAGGAGAAATCCGCCCAAGGAGGGGCTCGCGTCTGATTAGCTAGTT 6 0.15 No Hit GTCAAATCCCAGGGCTCAACCCTGGACAGGCGGTGGAAACTACCAAGCTG 6 0.15 No Hit CTTCCAATATGCTAGTTTCAACAACTCTCGTTCTTTACACTTCTTCTTGG 6 0.15 No Hit AGAACCTTACCAGGGCTTGACATGCCGCGAATCCTCTTGAAAGAGAGGGG 6 0.15 No Hit CAGGGCTTGACATGCCGCGAATCCTCTTGAAAGAGAGGGGTGCCCTCGGG 5 0.125 No Hit GTCAAGGAAGCTTCTCTGATGGTATGCCTTTAGGAATATCTGGTACTTTC 5 0.125 No Hit CGGTATCTGAGGAATAAGCATCGGCTAACTCTGTGCCAGCAGCCGCGGTA 5 0.125 No Hit TTCACATGTTAGGTGTAGCTGGTGTTTTCGGCGGTTCCCTATTCAGTGCT 5 0.125 No Hit CTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGAT 5 0.125 No Hit GGCTGATCTTCCCCAAGAGTCCACATCGACGGGAAGGTTTGGCACCTCGA 5 0.125 No Hit GGGAATTTCCGGTGGAGCGGTGAAATGCATTGAGATCGGAAAGAACACCA 5 0.125 No Hit TTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAG 5 0.125 No Hit GTGAAATGCCACTCGAACCCAGAGCTAGCTGGTTCTCCCCGAAATGCGTT 5 0.125 No Hit GGTTAAGTCTCGCAACGAGCGCAACCCTCGTGTTTAGTTGCCACTATGAG 5 0.125 No Hit CCGGAATGATTGGGCGTAAAGCGTCTGTAGGTGGCTTTTCAAGTCCGCCG 5 0.125 No Hit CTGACACTGAGAGACGAAAGCTAGGGGAGCAAATGGGATTAGAGACCCCA 5 0.125 No Hit GTTGATAGTCAAGGTCGCGTTATTAATACTTGGGCTGATATCATCAACCG 5 0.125 No Hit AGTCAAGGTCGCGTTATTAATACTTGGGCTGATATCATCAACCGTGCTAA 5 0.125 No Hit GTTGCATATTCAGCTCCTGTTGCAGCTGCGACTGCTGTTTTCTTGATTTA 5 0.125 No Hit GGGAAGTGGTGTTTCCAGTGGCGAACGGGTGAGTAACGCGTAAGAACCTG 5 0.125 No Hit GACCAGGCCCGGGCGACTGTTTACCAAAAACACAGGTCTCCGCAAAGTCG 5 0.125 No Hit CATCAACCGTGCTAATCTTGGTATGGAAGTAATGCACGAACGTAATGCTC 5 0.125 No Hit CCCAAATAGGTCAACCTTTTAAACTGCCTGCTGAATCCATGAGCAGGCAA 5 0.125 No Hit CCTGAACAGACCGCCGGTGTTAAGCCGGAGGAAGGAGAGGATGAGGCCAA 5 0.125 No Hit GCACAACATCCTTATGCATCCATTTCACATGTTAGGTGTAGCTGGTGTTT 5 0.125 No Hit AATGCATTGAGATCGGAAAGAACACCAACGGCGAAAGCACTCTGCTGGGC 5 0.125 No Hit TCTATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGA 5 0.125 No Hit GGCAGAGGGAATTTCCGGTGGAGCGGTGAAATGCATTGAGATCGGAAAGA 5 0.125 No Hit ATCTATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTG 5 0.125 No Hit GTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGATCTTACCATGACTG 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.025 0.0 0.0 0.0 0.0 70-71 0.025 0.0 0.0 0.0 0.0 72-73 0.075 0.0 0.0 0.0 0.0 74-75 0.1 0.0 0.0 0.0 0.0 76-77 0.1375 0.0 0.0 0.0 0.0 78-79 0.15 0.0 0.0 0.0 0.0 80-81 0.175 0.0 0.0 0.0 0.0 82-83 0.2375 0.0 0.0 0.0 0.0 84-85 0.3125 0.0 0.0 0.0 0.0 86-87 0.375 0.0 0.0 0.0 0.0 88-89 0.4375 0.0 0.0 0.0 0.0 90-91 0.575 0.0 0.0 0.0 0.0 92-93 0.6875 0.0 0.0 0.0 0.0 94-95 0.8999999999999999 0.0 0.0 0.0 0.0 96-97 1.15 0.0 0.0 0.0 0.0 98-99 1.4125 0.0 0.0 0.0 0.0 100-101 1.75 0.0 0.0 0.0 0.0 102-103 2.1625 0.0 0.0 0.0 0.0 104-105 2.5875 0.0 0.0 0.0 0.0 106-107 3.05 0.0 0.0 0.0 0.0 108-109 3.375 0.0 0.0 0.0 0.0 110-111 4.025 0.0 0.0 0.0 0.0 112-113 4.475 0.0 0.0 0.0 0.0 114-115 5.0375 0.0 0.0 0.0 0.0 116-117 5.6875 0.0 0.0 0.0 0.0 118-119 6.4 0.0 0.0 0.0 0.0 120-121 7.2 0.0 0.0 0.0 0.0 122-123 7.775 0.0 0.0 0.0 0.0 124-125 8.7 0.0 0.0 0.0 0.0 126-127 9.524999999999999 0.0 0.0 0.0 0.0 128-129 10.5125 0.0 0.0 0.0 0.0 130-131 11.3 0.0 0.0 0.0 0.0 132-133 12.15 0.0 0.0 0.0 0.0 134-135 13.15 0.0 0.0 0.0 0.0 136-137 14.025 0.0 0.0 0.0 0.0 138-139 15.024999999999999 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATCCTGG 10 0.006830828 145.0 6 TCGATCC 10 0.006830828 145.0 3 TTCGATC 10 0.006830828 145.0 2 CTGGCTC 10 0.006830828 145.0 9 GATCCTG 10 0.006830828 145.0 5 TCCTGGC 10 0.006830828 145.0 7 CCTGGCT 10 0.006830828 145.0 8 GTTCGAT 10 0.006830828 145.0 1 CGATCCT 10 0.006830828 145.0 4 >>END_MODULE Read 821787 spots for SRR6941592.sra Written 821787 spots for SRR6941592.sra Read 821787 spots for SRR6941592.sra Written 821787 spots for SRR6941592.sra Read 821787 spots for SRR6941592.sra Written 821787 spots for SRR6941592.sra Read 821787 spots for SRR6941592.sra Written 821787 spots for SRR6941592.sra Read 821787 spots for SRR6941592.sra Written 821787 spots for SRR6941592.sra Read 821787 spots for SRR6941592.sra Written 821787 spots for SRR6941592.sra Read 821787 spots for SRR6941592.sra Written 821787 spots for SRR6941592.sra Read 821787 spots for SRR6941592.sra Written 821787 spots for SRR6941592.sra Read 821787 spots for SRR6941592.sra Written 821787 spots for SRR6941592.sra Read 821787 spots for SRR6941592.sra Written 821787 spots for SRR6941592.sra Read 821787 spots for SRR6941592.sra Written 821787 spots for SRR6941592.sra Read 821800 spots for SRR6941592.sra Written 821800 spots for SRR6941592.sra Read 821787 spots for SRR6941592.sra Written 821787 spots for SRR6941592.sra Read 821787 spots for SRR6941592.sra Written 821787 spots for SRR6941592.sra Read 821787 spots for SRR6941592.sra Written 821787 spots for SRR6941592.sra Read 821787 spots for SRR6941592.sra Written 821787 spots for SRR6941592.sra Read 821787 spots for SRR6941592.sra Written 821787 spots for SRR6941592.sra Read 821787 spots for SRR6941592.sra Written 821787 spots for SRR6941592.sra Read 821787 spots for SRR6941592.sra Written 821787 spots for SRR6941592.sra Read 821787 spots for SRR6941592.sra Written 821787 spots for SRR6941592.sra SRR ids: ['SRR6941592.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_c9b6fwk5 SRR6941592.sra spots: 16435753 blocks: [[1, 821787], [821788, 1643574], [1643575, 2465361], [2465362, 3287148], [3287149, 4108935], [4108936, 4930722], [4930723, 5752509], [5752510, 6574296], [6574297, 7396083], [7396084, 8217870], [8217871, 9039657], [9039658, 9861444], [9861445, 10683231], [10683232, 11505018], [11505019, 12326805], [12326806, 13148592], [13148593, 13970379], [13970380, 14792166], [14792167, 15613953], [15613954, 16435753]] SRR6941592 file size 5547837 SRR6941592 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR6941592 SRR6941592_1.fastq SRR6941592_2.fastq Input file: SRR6941592_1.fastq Paired file: SRR6941592_2.fastq trimmed: SRR6941592-trimmed-pair1.fastq, SRR6941592-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Fri Dec 6 12:25:11 2024 >> started Fri Dec 6 12:25:38 2024 >> done (26.202s) 16435753 read pairs processed; of these: 5549 ( 0.03%) short read pairs filtered out after trimming by size control 4764 ( 0.03%) empty read pairs filtered out after trimming by size control 16425440 (99.94%) read pairs available; of these: 8562175 (52.13%) trimmed read pairs available after processing 7863265 (47.87%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 1 0.00% 19 3 0.00% 20 2 0.00% 21 1 0.00% 22 3 0.00% 23 1 0.00% 24 4 0.00% 25 9 0.00% 26 3 0.00% 27 11 0.00% 28 3 0.00% 29 7 0.00% 30 10 0.00% 31 12 0.00% 32 8 0.00% 33 9 0.00% 34 11 0.00% 35 12 0.00% 36 8 0.00% 37 9 0.00% 38 7 0.00% 39 18 0.00% 40 27 0.00% 41 35 0.00% 42 33 0.00% 43 19 0.00% 44 32 0.00% 45 27 0.00% 46 42 0.00% 47 32 0.00% 48 61 0.00% 49 67 0.00% 50 77 0.00% 51 115 0.00% 52 112 0.00% 53 160 0.00% 54 147 0.00% 55 178 0.00% 56 228 0.00% 57 213 0.00% 58 295 0.00% 59 339 0.00% 60 379 0.00% 61 463 0.00% 62 598 0.00% 63 672 0.00% 64 770 0.00% 65 853 0.01% 66 999 0.01% 67 1083 0.01% 68 1289 0.01% 69 1341 0.01% 70 1636 0.01% 71 1996 0.01% 72 2313 0.01% 73 2796 0.02% 74 2967 0.02% 75 3489 0.02% 76 3880 0.02% 77 4398 0.03% 78 4862 0.03% 79 5889 0.04% 80 6647 0.04% 81 7251 0.04% 82 8013 0.05% 83 9280 0.06% 84 10153 0.06% 85 12054 0.07% 86 12890 0.08% 87 13953 0.08% 88 15973 0.10% 89 16558 0.10% 90 18344 0.11% 91 19059 0.12% 92 21842 0.13% 93 23036 0.14% 94 23833 0.15% 95 27247 0.17% 96 27280 0.17% 97 30618 0.19% 98 31458 0.19% 99 34211 0.21% 100 34456 0.21% 101 38043 0.23% 102 38041 0.23% 103 39191 0.24% 104 41401 0.25% 105 42477 0.26% 106 44491 0.27% 107 46590 0.28% 108 50321 0.31% 109 51898 0.32% 110 51051 0.31% 111 51689 0.31% 112 53676 0.33% 113 53694 0.33% 114 57654 0.35% 115 60746 0.37% 116 61790 0.38% 117 60977 0.37% 118 62332 0.38% 119 61302 0.37% 120 65831 0.40% 121 67915 0.41% 122 70367 0.43% 123 73761 0.45% 124 72389 0.44% 125 77782 0.47% 126 75332 0.46% 127 77829 0.47% 128 77044 0.47% 129 79936 0.49% 130 77006 0.47% 131 81393 0.50% 132 82631 0.50% 133 82714 0.50% 134 85186 0.52% 135 85598 0.52% 136 89641 0.55% 137 89430 0.54% 138 94988 0.58% 139 99002 0.60% 140 100914 0.61% 141 112525 0.69% 142 114343 0.70% 143 124621 0.76% 144 137252 0.84% 145 160482 0.98% 146 182682 1.11% 147 228923 1.39% 148 325039 1.98% 149 611517 3.72% 150 3441518 20.95% 151 7863265 47.87% 16425440 reads passed initial QC criterion=sequence-density sequence-density=0.74 sequence-density-rank=1 fanout-score=4.97 fanout-score-rank=16 prefix-density=1.98 prefix-fanout=1.9 sequence=ATTTAGCCTTGGACGGAGTCTACCGCCCGATTTGGGCTGCATTCCCAAACAACCCGACTCGTTGACGGCGCCTCGTGGGGCGACAGGGTCCGGGCCGGACGGGGCTCTCACCCTCCCAGGCGCCCCTTTCCAGGGGACTTGGGCCCGGTCCGTCGCTGAGGACGCCTCTCCAGACTACAATTCGGACGGCACGGCCGCCCGATTCTCAAGCTGGGCTGCTCCCGGTTCGCTCGCCGTTACTAGGGGAATCCTTGTAAGTTTCTTCTCCTCCGCTTATTTATATGCTTAAACTCAGCGGGTAGTCCCGCCTGACC criterion=fanout-score sequence-density=0.01 sequence-density-rank=39 fanout-score=311.02 fanout-score-rank=1 prefix-density=1.93 prefix-fanout=1.0 sequence=GTAGAACAAGATATTGGGTATTTCTCGCTTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCATTAATAGCGGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTACGTTCGTGCATTACTTCCATACCAAGATTAGCACGGTTGATGATATCAGCCCAAGTATTAATAACGCGACCTTGACTATCAACTACAGATTGGTTGAAATTGAATCCATTTAGGTTGAACGCCATAGTACTAATACCTAAAGCAGTGAACCAGATTCCTACTACAGGCCAAGCAGCCAAGAAGAAGTGTAAAGAACGAGAGTTGTTGAAACTAGCATATTGGAAGATTAATCGGCCAAAATAACCATGAGCAGCCACAATATTATAAGTCTCTTCCTCTTGACCAAATTTGTAACCCTCATTAGCAGATTCATTTTCAGTAGTTTCCCTGATCAAACTAGAGGTTACCAAGGAACCATGCATAGCACTGAATAGGGAACCG criterion=sequence-density sequence-density=0.65 sequence-density-rank=1 fanout-score=7.97 fanout-score-rank=7 prefix-density=3.69 prefix-fanout=1.4 sequence=TGGTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGTTAACGAACGAGACCTCAGCCTGCTAACTAGCTATGCGGAGCCATCCCTCCGCAGCTAGCTTCTTAGAGGGACTATCGCCGTTTAGGCGACGGAAGTTTGAGGCAATAACAGGTCTGTGATGCCCTTAGATGTTCTGGGCCGCACGCGCGCTACACTGATGTATTCAACGAGTATATAGCCTTGGCCGACAGGCCCGGGTAATCTTGGGAAATTTCATCGTGATGGGGATAGATCATTGCAATTGTTGGTCTTCAACGAGGAATGCCTAGTAAGCGCGAGTCATCAGCTCGCGTTGACTACGTCCCTGCCCTTTGTACACACC criterion=fanout-score sequence-density=0.02 sequence-density-rank=33 fanout-score=64.02 fanout-score-rank=1 prefix-density=0.91 prefix-fanout=1.1 sequence=AGAAGGGGTGCCCCCTCACAAAAGGGGGTCGCAGTGACCAGGCCCGGGCGACTGTTTACCAAAAACACAGGTCTCCGCAAAGTCGTAAGACCATGTATGGGGGCTGACGCCTGCCCAGTGCCGGAAGGTCAAGGAAGTTGGTGAACTGATGACAGGGAAGCCGGCGACCGAAGCCCCGGTGAACGGCGGCCGTAAC SRR6941592 testing PE reads STAR mapping to Ensembl genome Started job on | Dec 06 12:26:19 Started mapping on | Dec 06 12:26:20 Finished on | Dec 06 12:27:43 Mapping speed, Million of reads per hour | 712.43 Number of input reads | 16425440 Average input read length | 288 UNIQUE READS: Uniquely mapped reads number | 7570775 Uniquely mapped reads % | 46.09% Average mapped length | 292.63 Number of splices: Total | 1084840 Number of splices: Annotated (sjdb) | 974836 Number of splices: GT/AG | 1036379 Number of splices: GC/AG | 12655 Number of splices: AT/AC | 3838 Number of splices: Non-canonical | 31968 Mismatch rate per base, % | 0.15% Deletion rate per base | 0.01% Deletion average length | 1.59 Insertion rate per base | 0.01% Insertion average length | 1.75 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 6634411 % of reads mapped to multiple loci | 40.39% Number of reads mapped to too many loci | 329969 % of reads mapped to too many loci | 2.01% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 2.72% % of reads unmapped: other | 8.79% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 2223373 2223373 2223373 N_multimapping 6634411 6634411 6634411 N_noFeature 4340685 7384458 4412678 N_ambiguous 226590 3641 114941 UnstrandedReadsAssigned:3003500 PositiveStrandReadsAssigned:182676 NegativeStrandReadsAssigned:3043156 Dataset is classified negative stranded MeadianReadLen=151 20thPercentileLength=144 echo kmer=139 SRR6941592 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,972 [index] number of k-mers: 66,720,672 [index] number of equivalence classes: 111,837 [quant] running in paired-end mode [quant] will process pair 1: SRR6941592-trimmed-pair1.fastq SRR6941592-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 16,425,440 reads, 6,148,437 reads pseudoaligned [quant] estimated average fragment length: 198.847 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 986 rounds 52973 SRR6941592.ke.tsv 35125 SRR6941592.se.tsv 88098 total ==> SRR6941592.ke.tsv <== target_id length eff_length est_counts tpm PNS24245 936 738.607 0 0 PNS24247 1044 846.153 3.94973 0.45709 PNS24249 1928 1730.15 3.1508 0.178328 PNS24246 1044 846.153 3.94973 0.45709 PNS24248 1044 846.153 3.94973 0.45709 PNS24244 1471 1273.15 0 0 PNS24243 293 121.65 1 0.804951 KQK14069 1603 1405.15 616.3 42.9489 KQK14071 474 283.712 9.77397 3.37347 ==> SRR6941592.se.tsv <== BRADI_1g14170v3 733 BRADI_1g53295v3 8 BRADI_1g59795v3 5 BRADI_1g07683v3 0 BRADI_1g00485v3 0 BRADI_1g20270v3 16 BRADI_1g74790v3 10 BRADI_1g09890v3 0 BRADI_1g77505v3 20 BRADI_1g48960v3 0 SRR6941592 completed mapping pipeline successfully