Starting /dee2/code/volunteer_pipeline.sh SRR6941593 current disk space = 1551213842432 free memory = 1600803104 SRR6941593 SRAfilesize 23028512f2e5e48d5fa6210ec5089655 SRR6941593.sra SRR6941593.sra file validated SRR6941593 is paired end SRR6941593 is conventional basespace SRR6941593 read1 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR6941593_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.76325 34.0 33.0 34.0 32.0 34.0 2 33.16325 34.0 33.0 34.0 32.0 34.0 3 33.225 34.0 33.0 34.0 32.0 34.0 4 33.402 34.0 33.0 34.0 33.0 34.0 5 33.4965 34.0 33.0 34.0 33.0 34.0 6 37.31875 38.0 38.0 38.0 36.0 38.0 7 37.65225 38.0 38.0 38.0 38.0 38.0 8 37.70075 38.0 38.0 38.0 38.0 38.0 9 37.67425 38.0 38.0 38.0 38.0 38.0 10-14 37.63505 38.0 38.0 38.0 38.0 38.0 15-19 37.6985 38.0 38.0 38.0 38.0 38.0 20-24 37.69905 38.0 38.0 38.0 38.0 38.0 25-29 37.624100000000006 38.0 38.0 38.0 38.0 38.0 30-34 37.5541 38.0 38.0 38.0 38.0 38.0 35-39 37.57025 38.0 38.0 38.0 38.0 38.0 40-44 37.576750000000004 38.0 38.0 38.0 38.0 38.0 45-49 37.579 38.0 38.0 38.0 38.0 38.0 50-54 37.544450000000005 38.0 38.0 38.0 38.0 38.0 55-59 37.557399999999994 38.0 38.0 38.0 38.0 38.0 60-64 37.51215 38.0 38.0 38.0 38.0 38.0 65-69 37.35345 38.0 38.0 38.0 37.0 38.0 70-74 37.326550000000005 38.0 38.0 38.0 37.0 38.0 75-79 37.397949999999994 38.0 38.0 38.0 37.0 38.0 80-84 37.30885 38.0 38.0 38.0 37.0 38.0 85-89 37.152049999999996 38.0 38.0 38.0 36.4 38.0 90-94 37.150400000000005 38.0 38.0 38.0 36.2 38.0 95-99 37.027550000000005 38.0 38.0 38.0 35.8 38.0 100-104 37.10095 38.0 38.0 38.0 36.0 38.0 105-109 36.936150000000005 38.0 38.0 38.0 35.6 38.0 110-114 36.5033 38.0 38.0 38.0 34.6 38.0 115-119 36.4841 38.0 38.0 38.0 34.6 38.0 120-124 36.51665 38.0 38.0 38.0 34.4 38.0 125-129 36.5469 38.0 38.0 38.0 34.4 38.0 130-134 36.431349999999995 38.0 38.0 38.0 34.2 38.0 135-139 36.01145 38.0 37.4 38.0 33.2 38.0 140-144 35.7804 38.0 36.4 38.0 32.8 38.0 145-149 35.40675 38.0 36.0 38.0 31.0 38.0 150-151 31.253124999999997 35.5 30.5 38.0 16.5 38.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 1.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 1.0 14 0.0 15 1.0 16 0.0 17 0.0 18 0.0 19 2.0 20 5.0 21 0.0 22 5.0 23 6.0 24 3.0 25 6.0 26 7.0 27 5.0 28 18.0 29 24.0 30 25.0 31 33.0 32 34.0 33 77.0 34 115.0 35 168.0 36 414.0 37 3050.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 42.04124801692226 14.04019037546272 6.689582231623479 37.228979375991536 2 24.5311327831958 14.928732183045762 34.058514628657164 26.481620405101275 3 20.025000000000002 21.95 27.500000000000004 30.525000000000002 4 24.9 30.275000000000002 22.375 22.45 5 23.575 34.775 22.375 19.275000000000002 6 19.15 34.425 24.05 22.375 7 14.399999999999999 24.474999999999998 42.449999999999996 18.675 8 17.775 23.175 29.375 29.675 9 17.95 19.950000000000003 32.525 29.575000000000003 10-14 21.73 28.04 24.26 25.97 15-19 22.38 26.31 25.865 25.445 20-24 21.349999999999998 27.060000000000002 25.965 25.624999999999996 25-29 22.400000000000002 26.22 26.555 24.825 30-34 22.14 27.985 25.14 24.735 35-39 22.472247224722473 26.112611261126112 26.8026802680268 24.61246124612461 40-44 21.408211231684753 26.563984597689654 26.393959093864076 25.633845076761514 45-49 21.255 26.75 27.08 24.915000000000003 50-54 21.699339867973595 26.46029205841168 25.975195039007804 25.86517303460692 55-59 21.202120212021203 26.792679267926793 26.44264426442644 25.562556255625562 60-64 21.372137213721373 26.017601760176017 26.767676767676768 25.842584258425845 65-69 21.275 26.515 25.985000000000003 26.224999999999998 70-74 23.201160058002902 26.87634381719086 24.71123556177809 25.211260563028155 75-79 21.59 27.1 25.585 25.724999999999998 80-84 22.662266226622663 26.422642264226422 25.73257325732573 25.18251825182518 85-89 21.675 26.045 26.915 25.365 90-94 21.73717371737174 27.217721772177217 24.897489748974895 26.147614761476145 95-99 21.12 27.61 24.945 26.325 100-104 22.134426885377074 27.635527105421083 24.88497699539908 25.345069013802764 105-109 21.89 26.584999999999997 25.77 25.755 110-114 21.890372452565003 26.764381086236323 26.22226684067865 25.12297962052003 115-119 21.34014935097479 27.960707663008066 24.703052172605624 25.996090813411517 120-124 21.125068815374608 28.25183924728492 23.492317701816727 27.130774235523745 125-129 21.814362872574513 27.695539107821567 24.65993198639728 25.83016603320664 130-134 22.61339200880132 27.35410311546732 23.328499274891236 26.704005600840127 135-139 22.97 27.689999999999998 24.740000000000002 24.6 140-144 22.74 28.415000000000003 24.01 24.834999999999997 145-149 21.959999999999997 27.515 24.455 26.07 150-151 20.837500000000002 28.050000000000004 24.1375 26.974999999999998 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 1.5 20 3.0 21 2.5 22 3.5 23 4.0 24 3.0 25 5.0 26 5.5 27 9.0 28 14.0 29 13.5 30 13.5 31 19.5 32 27.5 33 33.5 34 32.0 35 33.5 36 81.5 37 159.5 38 167.5 39 148.0 40 170.5 41 187.5 42 168.5 43 152.5 44 152.5 45 155.0 46 139.0 47 110.5 48 100.0 49 87.0 50 98.5 51 118.0 52 118.0 53 130.5 54 165.5 55 205.0 56 200.5 57 158.5 58 144.5 59 122.0 60 84.0 61 64.0 62 42.5 63 31.5 64 27.5 65 17.0 66 9.0 67 8.0 68 9.5 69 9.0 70 7.5 71 6.5 72 6.0 73 3.5 74 1.5 75 2.0 76 3.0 77 2.5 78 1.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content warn #Base N-Count 1 5.45 2 0.025 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.01 40-44 0.015 45-49 0.0 50-54 0.02 55-59 0.01 60-64 0.01 65-69 0.0 70-74 0.005 75-79 0.0 80-84 0.01 85-89 0.0 90-94 0.01 95-99 0.0 100-104 0.02 105-109 0.0 110-114 0.38999999999999996 115-119 0.23500000000000001 120-124 0.095 125-129 0.02 130-134 0.015 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 71.125 #Duplication Level Percentage of deduplicated Percentage of total 1 83.30404217926186 59.25 2 9.244288224956062 13.15 3 3.093145869947276 6.6000000000000005 4 1.546572934973638 4.3999999999999995 5 1.054481546572935 3.75 6 0.45694200351493847 1.95 7 0.421792618629174 2.1 8 0.17574692442882248 1.0 9 0.1054481546572935 0.675 >10 0.5975395430579965 7.124999999999999 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCATTAAT 40 1.0 No Hit GGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTACGTT 35 0.8750000000000001 No Hit GTTGAAACTAGCATATTGGAAGATTAATCGGCCAAAATAACCATGAGCAG 26 0.65 No Hit GCCAAAATAACCATGAGCAGCCACAATATTATAAGTCTCTTCCTCTTGAC 20 0.5 No Hit GTACAAGGCCCGGGAACGGATTCACCGCCGTATGGCTGACCGGCGATTAC 19 0.475 No Hit GCGGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTACG 15 0.375 No Hit CGCAGAAGTAGGAATAATAGCACCAGAGATAATATTGTTTCCATAAAGTA 14 0.35000000000000003 No Hit GTCGGTTTCGGGTACAGGTACCCTTTTGTTGAAGGTCGTTCGAGCTTTTC 13 0.325 No Hit CTCCACGCGGCATTGCTCCGTCAGGCTTTCGCCCATTGCGGAAAATTCCC 13 0.325 No Hit GTTTACGGCTAGGACTACTGGGGTCTCTAATCCCATTTGCTCCCCTAGCT 13 0.325 No Hit GGCATGATGACTTGGCCTCATCCTCTCCTTCCTCCGGCTTAACACCGGCG 12 0.3 No Hit TTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCATTAATA 12 0.3 No Hit GCTGAATATGCAACAGCAATCCAAGGGCGCATACCCAAACGGAAACTAAG 11 0.27499999999999997 No Hit CAAGTATTAATAACGCGACCTTGACTATCAACTACAGATTGGTTGAAATT 11 0.27499999999999997 No Hit GTGCTCTGCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTCCTA 11 0.27499999999999997 No Hit GTCAATTCCTTTGAGTTTCATTCTTGCGAACGTACTCCCCAGGCGGGATA 10 0.25 No Hit GTTTCTTCTCCGAGAAAAGAAGTTGACGACCCGTAGGCCTTCCACCTCCA 10 0.25 No Hit CTCCACCGCTTGTGCGGGCCCCCGTCAATTCCTTTGAGTTTCATTCTTGC 9 0.22499999999999998 No Hit GGAAAAACCTTATCCATTAATAGCGGGAACTTCAAGAGCAGCTAGATCTA 9 0.22499999999999998 No Hit CTTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCAT 9 0.22499999999999998 No Hit GGCCAAAATAACCATGAGCAGCCACAATATTATAAGTCTCTTCCTCTTGA 8 0.2 No Hit GCTCCACCGCTTGTGCGGGCCCCCGTCAATTCCTTTGAGTTTCATTCTTG 8 0.2 No Hit GCTACACCTAACATGTGAAATGGATGCATAAGGATGTTGTGCTCTGCCTG 8 0.2 No Hit CTCATAAGGACCACCATTGTATAACCATTCATCAACGGATGCAGCTTCCC 8 0.2 No Hit GCTTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCA 8 0.2 No Hit CGGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTACGT 7 0.17500000000000002 No Hit GCACGGTTGATGATATCAGCCCAAGTATTAATAACGCGACCTTGACTATC 7 0.17500000000000002 No Hit GCCGAAAACACCAGCTACACCTAACATGTGAAATGGATGCATAAGGATGT 7 0.17500000000000002 No Hit GTGAGCATTACGTTCGTGCATTACTTCCATACCAAGATTAGCACGGTTGA 7 0.17500000000000002 No Hit GCCAGCTCCTATAGTGTGACGGGCGGTGTGTACAAGGCCCGGGAACGGAT 7 0.17500000000000002 No Hit GGATGCATAAGGATGTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAA 7 0.17500000000000002 No Hit CATGCTCCACCGCTTGTGCGGGCCCCCGTCAATTCCTTTGAGTTTCATTC 7 0.17500000000000002 No Hit CATATAACAAGCTACACCAAGTAAGAAGTGTAGAACAATTAGCTCATAAG 7 0.17500000000000002 No Hit CTCATTAGCAGATTCATTTTCAGTAGTTTCCCTGATCAAACTAGAGGTTA 7 0.17500000000000002 No Hit GGTGTGTACAAGGCCCGGGAACGGATTCACCGCCGTATGGCTGACCGGCG 7 0.17500000000000002 No Hit GCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTCCTAAAGGCAT 7 0.17500000000000002 No Hit GGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTACGTTC 7 0.17500000000000002 No Hit CAGAGAAGCTTCCTTGACCAATAGGGTAAATCAAGAAAACAGCAGTCGCA 6 0.15 No Hit AGAGATAATATTGTTTCCATAAAGTAAAGAACCAGAAACAGGCTCGCGAA 6 0.15 No Hit GCGACCTTGACTATCAACTACAGATTGGTTGAAATTGAATCCATTTAGGT 6 0.15 No Hit GTGTGTACAAGGCCCGGGAACGGATTCACCGCCGTATGGCTGACCGGCGA 6 0.15 No Hit CCAAAATAACCATGAGCAGCCACAATATTATAAGTCTCTTCCTCTTGACC 6 0.15 No Hit CCCACTGCTGCCTCCCGTAGGAGTCTGGGCCGTGTCTCAGTCCCAGTGTG 6 0.15 No Hit GGGCTTACTACTTATATGCTTTCAGCAGTTATCCTCTCCGCACTTGGCTA 6 0.15 No Hit CTCAGATACCGTCATTGTTTCTTCTCCGAGAAAAGAAGTTGACGACCCGT 6 0.15 No Hit CTCAGTGTCAGTGTCGGCCCAGCAGAGTGCTTTCGCCGTTGGTGTTCTTT 6 0.15 No Hit GATGCATAAGGATGTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAAA 6 0.15 No Hit GCGGAAAAACCTTATCCATTAATAGCGGGAACTTCAAGAGCAGCTAGATC 6 0.15 No Hit CCCCACTGCTGCCTCCCGTAGGAGTCTGGGCCGTGTCTCAGTCCCAGTGT 6 0.15 No Hit ACCACATGCTCCACCGCTTGTGCGGGCCCCCGTCAATTCCTTTGAGTTTC 6 0.15 No Hit CACCGCTTGTGCGGGCCCCCGTCAATTCCTTTGAGTTTCATTCTTGCGAA 5 0.125 No Hit CCTAGATGTCCAGTCAACTGCTGCGCCTCAACGCATTTCGGGGAGAACCA 5 0.125 No Hit AGAGAAGCTTCCTTGACCAATAGGGTAAATCAAGAAAACAGCAGTCGCAG 5 0.125 No Hit GGTTGATGATATCAGCCCAAGTATTAATAACGCGACCTTGACTATCAACT 5 0.125 No Hit CGAGAGTTGTTGAAACTAGCATATTGGAAGATTAATCGGCCAAAATAACC 5 0.125 No Hit GGATAACGCTTGCATCCTCTGTCTTACCGCGGCTGCTGGCACAGAGTTAG 5 0.125 No Hit GTGTACAAGGCCCGGGAACGGATTCACCGCCGTATGGCTGACCGGCGATT 5 0.125 No Hit ACTCACGACCCATATAACAAGCTACACCAAGTAAGAAGTGTAGAACAATT 5 0.125 No Hit ATAAGGACCACCATTGTATAACCATTCATCAACGGATGCAGCTTCCCAAA 5 0.125 No Hit CAAGGCCCGGGAACGGATTCACCGCCGTATGGCTGACCGGCGATTACTAG 5 0.125 No Hit CCCAAGTATTAATAACGCGACCTTGACTATCAACTACAGATTGGTTGAAA 5 0.125 No Hit CCAGATTCCTACTACAGGCCAAGCAGCCAAGAAGAAGTGTAAAGAACGAG 5 0.125 No Hit GGATCATCAAAACACCGAACCATCCGATGTAAAGACGATTTTCAGTGCTA 5 0.125 No Hit AGGAATAATAGCACCAGAGATAATATTGTTTCCATAAAGTAAAGAACCAG 5 0.125 No Hit GCTGCGATGAAGGCGATAATAAATACAGAAGTTGCGGTCAATAAGGTAGG 5 0.125 No Hit GGATGTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAAAGTACCAGAT 5 0.125 No Hit GGAAGTTGTGAGCATTACGTTCGTGCATTACTTCCATACCAAGATTAGCA 5 0.125 No Hit TGAGCATTACGTTCGTGCATTACTTCCATACCAAGATTAGCACGGTTGAT 5 0.125 No Hit GTTAGCGGAAAAACCTTATCCATTAATAGCGGGAACTTCAAGAGCAGCTA 5 0.125 No Hit CAGATTCCTACTACAGGCCAAGCAGCCAAGAAGAAGTGTAAAGAACGAGA 5 0.125 No Hit GTGCTAGTTATCCAGTTGCAGAAGCGACCCCACAGGCTTGTACTTTCGCG 5 0.125 No Hit AGATATTCCTAAAGGCATACCATCAGAGAAGCTTCCTTGACCAATAGGGT 5 0.125 No Hit GTAACTTTTATCCGTTGAGCGACGGCCCTTCCACTCGGCACCGTCGGATC 5 0.125 No Hit CATAAGGATGTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAAAGTAC 5 0.125 No Hit GGTGTGTACAAAGGGCAGGGACGTAGTCAACGCGAGCTGATGACTCGCGC 5 0.125 No Hit CTTCACTCCAGTCGCAAGCCTAGCCTTAGGCATCCCCCTCCTTACGGTTA 5 0.125 No Hit GTCCAGTCAACTGCTGCGCCTCAACGCATTTCGGGGAGAACCAGCTAGCT 5 0.125 No Hit CCTGTGTCGGTTTCGGGTACAGGTACCCTTTTGTTGAAGGTCGTTCGAGC 5 0.125 No Hit CTTTCTTTTCCTCTGGCTACTAAGATGTTTCAGTTCGCCAGGTTGTCTCT 5 0.125 No Hit GCCAAGAAGAAGTGTAAAGAACGAGAGTTGTTGAAACTAGCATATTGGAA 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0125 0.0 0.0 0.0 0.0 62-63 0.037500000000000006 0.0 0.0 0.0 0.0 64-65 0.05 0.0 0.0 0.0 0.0 66-67 0.0625 0.0 0.0 0.0 0.0 68-69 0.075 0.0 0.0 0.0 0.0 70-71 0.075 0.0 0.0 0.0 0.0 72-73 0.1 0.0 0.0 0.0 0.0 74-75 0.125 0.0 0.0 0.0 0.0 76-77 0.125 0.0 0.0 0.0 0.0 78-79 0.16249999999999998 0.0 0.0 0.0 0.0 80-81 0.25 0.0 0.0 0.0 0.0 82-83 0.3125 0.0 0.0 0.0 0.0 84-85 0.38749999999999996 0.0 0.0 0.0 0.0 86-87 0.5125 0.0 0.0 0.0 0.0 88-89 0.5375000000000001 0.0 0.0 0.0 0.0 90-91 0.7125 0.0 0.0 0.0 0.0 92-93 0.8625 0.0 0.0 0.0 0.0 94-95 1.0499999999999998 0.0 0.0 0.0 0.0 96-97 1.2875 0.0 0.0 0.0 0.0 98-99 1.55 0.0 0.0 0.0 0.0 100-101 1.775 0.0 0.0 0.0 0.0 102-103 2.175 0.0 0.0 0.0 0.0 104-105 2.6500000000000004 0.0 0.0 0.0 0.0 106-107 2.95 0.0 0.0 0.0 0.0 108-109 3.375 0.0 0.0 0.0 0.0 110-111 3.7125 0.0 0.0 0.0 0.0 112-113 4.25 0.0 0.0 0.0 0.0 114-115 4.762499999999999 0.0 0.0 0.0 0.0 116-117 5.2875 0.0 0.0 0.0 0.0 118-119 6.0125 0.0 0.0 0.0 0.0 120-121 6.6375 0.0 0.0 0.0 0.0 122-123 7.15 0.0 0.0 0.0 0.0 124-125 7.7875 0.0 0.0 0.0 0.0 126-127 8.5625 0.0 0.0 0.0 0.0 128-129 9.3 0.0 0.0 0.0 0.0 130-131 9.8625 0.0 0.0 0.0 0.0 132-133 10.6625 0.0 0.0 0.0 0.0 134-135 11.25 0.0 0.0 0.0 0.0 136-137 11.9125 0.0 0.0 0.0 0.0 138-139 12.6375 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTTTTCT 10 0.0056249425 154.6 1 CTCCACC 10 0.0056249425 154.6 1 CCGCTTG 10 0.0068396386 144.9375 6 TTTTCTT 10 0.0068396386 144.9375 2 CACCGCT 10 0.0068396386 144.9375 4 ACCGCTT 10 0.0068396386 144.9375 5 GCTTGTG 10 0.0068396386 144.9375 8 CTTGTGC 10 0.0068396386 144.9375 9 >>END_MODULE SRR6941593 read2 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR6941593_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 33.2825 34.0 33.0 34.0 33.0 34.0 2 33.32675 34.0 33.0 34.0 33.0 34.0 3 33.33875 34.0 33.0 34.0 33.0 34.0 4 33.39225 34.0 33.0 34.0 33.0 34.0 5 33.37625 34.0 33.0 34.0 33.0 34.0 6 37.55375 38.0 38.0 38.0 38.0 38.0 7 37.54075 38.0 38.0 38.0 38.0 38.0 8 37.5365 38.0 38.0 38.0 38.0 38.0 9 37.465 38.0 38.0 38.0 38.0 38.0 10-14 37.509150000000005 38.0 38.0 38.0 38.0 38.0 15-19 37.5151 38.0 38.0 38.0 38.0 38.0 20-24 37.50285 38.0 38.0 38.0 38.0 38.0 25-29 37.445750000000004 38.0 38.0 38.0 38.0 38.0 30-34 37.4966 38.0 38.0 38.0 38.0 38.0 35-39 37.4568 38.0 38.0 38.0 38.0 38.0 40-44 37.43535000000001 38.0 38.0 38.0 38.0 38.0 45-49 37.43315 38.0 38.0 38.0 38.0 38.0 50-54 37.40259999999999 38.0 38.0 38.0 38.0 38.0 55-59 37.34920000000001 38.0 38.0 38.0 37.8 38.0 60-64 37.29305 38.0 38.0 38.0 37.4 38.0 65-69 37.26275 38.0 38.0 38.0 37.0 38.0 70-74 37.270500000000006 38.0 38.0 38.0 37.2 38.0 75-79 37.21 38.0 38.0 38.0 37.0 38.0 80-84 37.1066 38.0 38.0 38.0 37.0 38.0 85-89 37.103500000000004 38.0 38.0 38.0 36.6 38.0 90-94 37.09955 38.0 38.0 38.0 36.8 38.0 95-99 37.044700000000006 38.0 38.0 38.0 36.0 38.0 100-104 36.750299999999996 38.0 38.0 38.0 35.2 38.0 105-109 36.58435 38.0 38.0 38.0 34.8 38.0 110-114 36.22375000000001 38.0 38.0 38.0 34.0 38.0 115-119 36.1656 38.0 38.0 38.0 33.8 38.0 120-124 36.01345 38.0 38.0 38.0 33.2 38.0 125-129 36.1297 38.0 38.0 38.0 33.6 38.0 130-134 36.16705 38.0 38.0 38.0 33.8 38.0 135-139 35.8974 38.0 37.4 38.0 32.6 38.0 140-144 35.271049999999995 38.0 36.0 38.0 31.0 38.0 145-149 34.2774 38.0 35.2 38.0 27.2 38.0 150-151 29.547375000000002 35.5 27.0 38.0 7.5 38.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 3.0 4 0.0 5 1.0 6 3.0 7 0.0 8 1.0 9 3.0 10 0.0 11 2.0 12 1.0 13 1.0 14 2.0 15 1.0 16 3.0 17 2.0 18 4.0 19 2.0 20 2.0 21 0.0 22 6.0 23 5.0 24 6.0 25 7.0 26 9.0 27 8.0 28 17.0 29 18.0 30 20.0 31 49.0 32 43.0 33 65.0 34 116.0 35 212.0 36 467.0 37 2919.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.8 19.25 12.174999999999999 24.775 2 31.175000000000004 18.575 31.424999999999997 18.825 3 21.2 23.1 35.8 19.900000000000002 4 26.650000000000002 32.324999999999996 22.875 18.15 5 27.224999999999998 33.95 21.325 17.5 6 23.0 35.4 22.15 19.45 7 19.45 19.375 39.875 21.3 8 22.375 23.125 26.125 28.375 9 25.025 20.05 29.125 25.8 10-14 26.815 24.75 26.3 22.134999999999998 15-19 26.345000000000002 25.52 27.089999999999996 21.044999999999998 20-24 26.265 25.095 27.325 21.315 25-29 25.218782817422614 25.778866830024505 26.8740311046657 22.12831924788718 30-34 26.416320816040802 25.36126806340317 27.05635281764088 21.166058302915143 35-39 26.21762176217622 26.712671267126716 25.91259125912591 21.157115711571155 40-44 26.040000000000003 26.25 25.990000000000002 21.72 45-49 26.3963198159908 26.111305565278265 26.151307565378268 21.341067053352667 50-54 26.19 25.35 27.075 21.385 55-59 25.935000000000002 26.369999999999997 26.334999999999997 21.36 60-64 26.14130706535327 24.91624581229061 27.681384069203457 21.261063053152657 65-69 26.237623762376238 25.152515251525152 27.062706270627064 21.547154715471546 70-74 26.77035407081416 24.589917983596717 27.38047609521904 21.259251850370074 75-79 26.266313315665784 25.061253062653133 27.1963598179909 21.476073803690184 80-84 26.515606242496997 25.140056022408963 27.32593037214886 21.018407362945176 85-89 26.487648764876486 25.677567756775677 25.872587258725872 21.962196219621962 90-94 26.064999999999998 25.430000000000003 26.840000000000003 21.665 95-99 26.14 25.605 26.87 21.385 100-104 26.395558223289317 25.97539015606242 26.60564225690276 21.0234093637455 105-109 27.284098869208446 25.12258581006705 26.738717101971382 20.854598218753125 110-114 26.01130056502825 25.711285564278214 26.681334066703332 21.5960798039902 115-119 26.48632431621581 25.906295314765735 26.511325566278316 21.096054802740134 120-124 26.200000000000003 26.724999999999998 25.15 21.925 125-129 26.45632281614081 26.136306815340767 25.321266063303167 22.086104305215258 130-134 26.7003400680136 26.285257051410284 26.010202040408082 21.004200840168032 135-139 26.50132506625331 26.251312565628282 26.75133756687834 20.49602480124006 140-144 27.159073861079165 25.85887883182477 26.87903185477822 20.103015452317848 145-149 27.45323597079124 26.698009402820844 25.792737821346407 20.056016805041512 150-151 27.76735459662289 25.365853658536587 26.816760475297063 20.050031269543464 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.5 19 0.5 20 0.0 21 1.0 22 2.5 23 3.5 24 6.0 25 9.5 26 9.5 27 9.5 28 14.5 29 23.0 30 26.0 31 21.0 32 22.0 33 26.5 34 42.0 35 54.0 36 73.5 37 108.0 38 124.0 39 146.5 40 171.5 41 166.0 42 160.5 43 172.5 44 170.5 45 142.5 46 135.0 47 137.0 48 120.5 49 104.0 50 92.0 51 101.5 52 96.5 53 126.0 54 183.0 55 188.0 56 180.5 57 148.5 58 128.0 59 126.5 60 92.0 61 70.0 62 69.5 63 52.0 64 28.5 65 20.0 66 15.5 67 16.0 68 18.0 69 11.0 70 7.0 71 8.0 72 5.0 73 2.0 74 1.5 75 2.5 76 4.0 77 2.5 78 0.5 79 0.5 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.015 30-34 0.005 35-39 0.01 40-44 0.0 45-49 0.005 50-54 0.0 55-59 0.0 60-64 0.005 65-69 0.01 70-74 0.02 75-79 0.005 80-84 0.04 85-89 0.01 90-94 0.0 95-99 0.0 100-104 0.04 105-109 0.06999999999999999 110-114 0.005 115-119 0.005 120-124 0.0 125-129 0.005 130-134 0.02 135-139 0.005 140-144 0.015 145-149 0.03 150-151 0.0625 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 70.92500000000001 #Duplication Level Percentage of deduplicated Percentage of total 1 81.63553048995418 57.9 2 9.975326048642932 14.149999999999999 3 4.053577722946774 8.625 4 1.5861825872400421 4.5 5 0.8107155445893549 2.875 6 0.8107155445893549 3.45 7 0.31723651744800846 1.575 8 0.24673951357067325 1.4000000000000001 9 0.035248501938667604 0.22499999999999998 >10 0.5287275290800141 5.3 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTTCGATCCTGGCTCAGGATGAACGCTGGCGGCATGCTTAACACATGCAA 31 0.775 No Hit CCTTACCAGGGCTTGACATGCCGCGAATCCTCTTGAAAGAGAGGGGTGCC 19 0.475 No Hit GGAAACTACTGAAAATGAATCTGCTAATGAGGGTTACAAATTTGGTCAAG 16 0.4 No Hit GTTCAACCTAAATGGATTCAATTTCAACCAATCTGTAGTTGATAGTCAAG 16 0.4 No Hit CCTGGCTCAGGATGAACGCTGGCGGCATGCTTAACACATGCAAGTCGAAC 14 0.35000000000000003 No Hit GCTGCGACTGCTGTTTTCTTGATTTACCCTATTGGTCAAGGAAGCTTCTC 14 0.35000000000000003 No Hit GGAAGCTTCTCTGATGGTATGCCTTTAGGAATATCTGGTACTTTCAACTT 13 0.325 No Hit GCACAACATCCTTATGCATCCATTTCACATGTTAGGTGTAGCTGGTGTTT 13 0.325 No Hit ATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAA 12 0.3 No Hit GGCTTGCGGTGGATACCTAGGCACCCAGAGACGAGGAAGGGCGTAGCAAG 12 0.3 No Hit AGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGATCT 11 0.27499999999999997 No Hit AGTGACCAGGCCCGGGCGACTGTTTACCAAAAACACAGGTCTCCGCAAAG 11 0.27499999999999997 No Hit GTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGATCTT 10 0.25 No Hit ATTTGGTCAAGAGGAAGAGACTTATAATATTGTGGCTGCTCATGGTTATT 10 0.25 No Hit GGTACTTTCAACTTTATGATTGTATTCCAGGCAGAGCACAACATCCTTAT 10 0.25 No Hit TGAAAATGAATCTGCTAATGAGGGTTACAAATTTGGTCAAGAGGAAGAGA 9 0.22499999999999998 No Hit TGGGAACTTAGTTTCCGTTTGGGTATGCGCCCTTGGATTGCTGTTGCATA 8 0.2 No Hit CTTGCGGTGGATACCTAGGCACCCAGAGACGAGGAAGGGCGTAGCAAGCG 8 0.2 No Hit GTTATTTTGGCCGATTAATCTTCCAATATGCTAGTTTCAACAACTCTCGT 8 0.2 No Hit GTGGGAACTTAGTTTCCGTTTGGGTATGCGCCCTTGGATTGCTGTTGCAT 8 0.2 No Hit GGGAACTTAGTTTCCGTTTGGGTATGCGCCCTTGGATTGCTGTTGCATAT 8 0.2 No Hit GTCAAGGTCGCGTTATTAATACTTGGGCTGATATCATCAACCGTGCTAAT 8 0.2 No Hit TATCTATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTT 8 0.2 No Hit GGTCGCTTCTGCAACTGGATAACTAGCACTGAAAATCGTCTTTACATCGG 7 0.17500000000000002 No Hit GCTAATTGTTCTACACTTCTTACTTGGTGTAGCTTGTTATATGGGTCGTG 7 0.17500000000000002 No Hit GGAATATCTGGTACTTTCAACTTTATGATTGTATTCCAGGCAGAGCACAA 7 0.17500000000000002 No Hit GCTTGTTATATGGGTCGTGAGTGGGAACTTAGTTTCCGTTTGGGTATGCG 7 0.17500000000000002 No Hit GGAAGGCCTACGGGTCGTCAACTTCTTTTCTCGGAGAAGAAACAATGACG 7 0.17500000000000002 No Hit CTTACCAGGGCTTGACATGCCGCGAATCCTCTTGAAAGAGAGGGGTGCCC 7 0.17500000000000002 No Hit GTTTGATCAGGGAAACTACTGAAAATGAATCTGCTAATGAGGGTTACAAA 7 0.17500000000000002 No Hit GTCGCGATCTCGCGAGGGTGAGCTAACTCCAAAAACCCGTCCTCAGTTCG 7 0.17500000000000002 No Hit TGATAGTCAAGGTCGCGTTATTAATACTTGGGCTGATATCATCAACCGTG 7 0.17500000000000002 No Hit AGCACAACATCCTTATGCATCCATTTCACATGTTAGGTGTAGCTGGTGTT 6 0.15 No Hit GCTGGCGGCATGCTTAACACATGCAAGTCGAACGGGAAGTGGTGTTTCCA 6 0.15 No Hit GTTTGGGTATGCGCCCTTGGATTGCTGTTGCATATTCAGCTCCTGTTGCA 6 0.15 No Hit CTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGAT 6 0.15 No Hit GTCTTTACATCGGATGGTTCGGTGTTTTGATGATCCCTACCTTATTGACC 6 0.15 No Hit GGTGTTTTGATGATCCCTACCTTATTGACCGCAACTTCTGTATTTATTAT 6 0.15 No Hit TATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAA 6 0.15 No Hit CCAAGATCTTACCATGACTGCAATTTTAGAGAGACGCGAAAGTACAAGCC 6 0.15 No Hit TTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAG 6 0.15 No Hit CTTGGTAACCTCTAGTTTGATCAGGGAAACTACTGAAAATGAATCTGCTA 6 0.15 No Hit GCTTGACATGCCGCGAATCCTCTTGAAAGAGAGGGGTGCCCTCGGGAACG 6 0.15 No Hit AGTCAAGGTCGCGTTATTAATACTTGGGCTGATATCATCAACCGTGCTAA 6 0.15 No Hit ATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCA 6 0.15 No Hit CAACAACTCTCGTTCTTTACACTTCTTCTTGGCTGCTTGGCCTGTAGTAG 6 0.15 No Hit ATTCAGTGCTATGCATGGTTCCTTGGTAACCTCTAGTTTGATCAGGGAAA 6 0.15 No Hit TCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGA 6 0.15 No Hit CAATGGTGGTCCTTATGAGCTAATTGTTCTACACTTCTTACTTGGTGTAG 6 0.15 No Hit GGATGATCAGCCACACTGGGACTGAGACACGGCCCAGACTCCTACGGGAG 6 0.15 No Hit TCTATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGA 6 0.15 No Hit GAAACAATGACGGTATCTGAGGAATAAGCATCGGCTAACTCTGTGCCAGC 6 0.15 No Hit GTACAAGCCTGTGGGGTCGCTTCTGCAACTGGATAACTAGCACTGAAAAT 6 0.15 No Hit GTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGATCTTACCATGAC 6 0.15 No Hit GTTCTACACTTCTTACTTGGTGTAGCTTGTTATATGGGTCGTGAGTGGGA 6 0.15 No Hit CAGGGCTTGACATGCCGCGAATCCTCTTGAAAGAGAGGGGTGCCCTCGGG 5 0.125 No Hit GGAATTTCCGGTGGAGCGGTGAAATGCATTGAGATCGGAAAGAACACCAA 5 0.125 No Hit ACCTAGGCACCCAGAGACGAGGAAGGGCGTAGCAAGCGACGAAATGCTTC 5 0.125 No Hit CCTACTTCTGCGGCAATCGGATTGCACTTTTACCCAATTTGGGAAGCTGC 5 0.125 No Hit TTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAA 5 0.125 No Hit ATCGGATTGCACTTTTACCCAATTTGGGAAGCTGCATCCGTTGATGAATG 5 0.125 No Hit CCTAAATGGATTCAATTTCAACCAATCTGTAGTTGATAGTCAAGGTCGCG 5 0.125 No Hit GGGAAACTACTGAAAATGAATCTGCTAATGAGGGTTACAAATTTGGTCAA 5 0.125 No Hit CATGACTGCAATTTTAGAGAGACGCGAAAGTACAAGCCTGTGGGGTCGCT 5 0.125 No Hit GTGACCAGGCCCGGGCGACTGTTTACCAAAAACACAGGTCTCCGCAAAGT 5 0.125 No Hit TGAAAATCGTCTTTACATCGGATGGTTCGGTGTTTTGATGATCCCTACCT 5 0.125 No Hit CTATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAA 5 0.125 No Hit ATTATCTCTGGTGCTATTATTCCTACTTCTGCGGCAATCGGATTGCACTT 5 0.125 No Hit ACCAGGGCTTGACATGCCGCGAATCCTCTTGAAAGAGAGGGGTGCCCTCG 5 0.125 No Hit TTCCAATATGCTAGTTTCAACAACTCTCGTTCTTTACACTTCTTCTTGGC 5 0.125 No Hit GGAATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCTAAA 5 0.125 No Hit GGAACTTAGTTTCCGTTTGGGTATGCGCCCTTGGATTGCTGTTGCATATT 5 0.125 No Hit GCCTGTAGTAGGAATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGT 5 0.125 No Hit GTTTCAACAACTCTCGTTCTTTACACTTCTTCTTGGCTGCTTGGCCTGTA 5 0.125 No Hit CATCAACCGTGCTAATCTTGGTATGGAAGTAATGCACGAACGTAATGCTC 5 0.125 No Hit GTTTCCGTTTGGGTATGCGCCCTTGGATTGCTGTTGCATATTCAGCTCCT 5 0.125 No Hit GCTAACTCCAAAAACCCGTCCTCAGTTCGGATTGCAGGCTGCAACTCGCC 5 0.125 No Hit GTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGATCTTACCATGACTG 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0125 0.0 0.0 0.0 0.0 62-63 0.037500000000000006 0.0 0.0 0.0 0.0 64-65 0.05 0.0 0.0 0.0 0.0 66-67 0.0625 0.0 0.0 0.0 0.0 68-69 0.075 0.0 0.0 0.0 0.0 70-71 0.075 0.0 0.0 0.0 0.0 72-73 0.1 0.0 0.0 0.0 0.0 74-75 0.125 0.0 0.0 0.0 0.0 76-77 0.125 0.0 0.0 0.0 0.0 78-79 0.16249999999999998 0.0 0.0 0.0 0.0 80-81 0.25 0.0 0.0 0.0 0.0 82-83 0.3125 0.0 0.0 0.0 0.0 84-85 0.38749999999999996 0.0 0.0 0.0 0.0 86-87 0.5125 0.0 0.0 0.0 0.0 88-89 0.5375000000000001 0.0 0.0 0.0 0.0 90-91 0.7125 0.0 0.0 0.0 0.0 92-93 0.8625 0.0 0.0 0.0 0.0 94-95 1.0375 0.0 0.0 0.0 0.0 96-97 1.2625 0.0 0.0 0.0 0.0 98-99 1.525 0.0 0.0 0.0 0.0 100-101 1.775 0.0 0.0 0.0 0.0 102-103 2.175 0.0 0.0 0.0 0.0 104-105 2.6375 0.0 0.0 0.0 0.0 106-107 2.95 0.0 0.0 0.0 0.0 108-109 3.4000000000000004 0.0 0.0 0.0 0.0 110-111 3.7375 0.0 0.0 0.0 0.0 112-113 4.275 0.0 0.0 0.0 0.0 114-115 4.7875 0.0 0.0 0.0 0.0 116-117 5.3125 0.0 0.0 0.0 0.0 118-119 6.025 0.0 0.0 0.0 0.0 120-121 6.6375 0.0 0.0 0.0 0.0 122-123 7.175000000000001 0.0 0.0 0.0 0.0 124-125 7.7875 0.0 0.0 0.0 0.0 126-127 8.5875 0.0 0.0 0.0 0.0 128-129 9.325 0.0 0.0 0.0 0.0 130-131 9.8875 0.0 0.0 0.0 0.0 132-133 10.6875 0.0 0.0 0.0 0.0 134-135 11.275 0.0 0.0 0.0 0.0 136-137 11.9125 0.0 0.0 0.0 0.0 138-139 12.6375 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content pass >>END_MODULE Read 937470 spots for SRR6941593.sra Written 937470 spots for SRR6941593.sra Read 937470 spots for SRR6941593.sra Written 937470 spots for SRR6941593.sra Read 937470 spots for SRR6941593.sra Written 937470 spots for SRR6941593.sra Read 937470 spots for SRR6941593.sra Written 937470 spots for SRR6941593.sra Read 937470 spots for SRR6941593.sra Written 937470 spots for SRR6941593.sra Read 937470 spots for SRR6941593.sra Written 937470 spots for SRR6941593.sra Read 937470 spots for SRR6941593.sra Written 937470 spots for SRR6941593.sra Read 937470 spots for SRR6941593.sra Written 937470 spots for SRR6941593.sra Read 937470 spots for SRR6941593.sra Written 937470 spots for SRR6941593.sra Read 937470 spots for SRR6941593.sra Written 937470 spots for SRR6941593.sra Read 937470 spots for SRR6941593.sra Written 937470 spots for SRR6941593.sra Read 937470 spots for SRR6941593.sra Written 937470 spots for SRR6941593.sra Read 937470 spots for SRR6941593.sra Written 937470 spots for SRR6941593.sra Read 937470 spots for SRR6941593.sra Written 937470 spots for SRR6941593.sra Read 937470 spots for SRR6941593.sra Written 937470 spots for SRR6941593.sra Read 937470 spots for SRR6941593.sra Written 937470 spots for SRR6941593.sra Read 937470 spots for SRR6941593.sra Written 937470 spots for SRR6941593.sra Read 937470 spots for SRR6941593.sra Written 937470 spots for SRR6941593.sra Read 937481 spots for SRR6941593.sra Written 937481 spots for SRR6941593.sra Read 937470 spots for SRR6941593.sra Written 937470 spots for SRR6941593.sra SRR ids: ['SRR6941593.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_o8zsana8 SRR6941593.sra spots: 18749411 blocks: [[1, 937470], [937471, 1874940], [1874941, 2812410], [2812411, 3749880], [3749881, 4687350], [4687351, 5624820], [5624821, 6562290], [6562291, 7499760], [7499761, 8437230], [8437231, 9374700], [9374701, 10312170], [10312171, 11249640], [11249641, 12187110], [12187111, 13124580], [13124581, 14062050], [14062051, 14999520], [14999521, 15936990], [15936991, 16874460], [16874461, 17811930], [17811931, 18749411]] SRR6941593 file size 6331859 SRR6941593 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR6941593 SRR6941593_1.fastq SRR6941593_2.fastq Input file: SRR6941593_1.fastq Paired file: SRR6941593_2.fastq trimmed: SRR6941593-trimmed-pair1.fastq, SRR6941593-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Fri Dec 6 12:32:29 2024 >> started Fri Dec 6 12:32:50 2024 >> done (20.722s) 18749411 read pairs processed; of these: 8412 ( 0.04%) short read pairs filtered out after trimming by size control 6057 ( 0.03%) empty read pairs filtered out after trimming by size control 18734942 (99.92%) read pairs available; of these: 9359038 (49.95%) trimmed read pairs available after processing 9375904 (50.05%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 3 0.00% 19 0 0.00% 20 4 0.00% 21 6 0.00% 22 0 0.00% 23 2 0.00% 24 2 0.00% 25 5 0.00% 26 5 0.00% 27 30 0.00% 28 6 0.00% 29 5 0.00% 30 4 0.00% 31 15 0.00% 32 10 0.00% 33 3 0.00% 34 7 0.00% 35 12 0.00% 36 10 0.00% 37 8 0.00% 38 8 0.00% 39 15 0.00% 40 19 0.00% 41 20 0.00% 42 21 0.00% 43 22 0.00% 44 25 0.00% 45 37 0.00% 46 32 0.00% 47 48 0.00% 48 54 0.00% 49 70 0.00% 50 84 0.00% 51 104 0.00% 52 113 0.00% 53 122 0.00% 54 124 0.00% 55 177 0.00% 56 174 0.00% 57 221 0.00% 58 264 0.00% 59 315 0.00% 60 386 0.00% 61 449 0.00% 62 593 0.00% 63 595 0.00% 64 758 0.00% 65 719 0.00% 66 826 0.00% 67 973 0.01% 68 1160 0.01% 69 1272 0.01% 70 1557 0.01% 71 1767 0.01% 72 2202 0.01% 73 2514 0.01% 74 2573 0.01% 75 3145 0.02% 76 3474 0.02% 77 3712 0.02% 78 4344 0.02% 79 5160 0.03% 80 5909 0.03% 81 6586 0.04% 82 7080 0.04% 83 8308 0.04% 84 8972 0.05% 85 11161 0.06% 86 11462 0.06% 87 12566 0.07% 88 15125 0.08% 89 15148 0.08% 90 16703 0.09% 91 16942 0.09% 92 19502 0.10% 93 20829 0.11% 94 21409 0.11% 95 24668 0.13% 96 24470 0.13% 97 26500 0.14% 98 27363 0.15% 99 28823 0.15% 100 29640 0.16% 101 32448 0.17% 102 33259 0.18% 103 33587 0.18% 104 37013 0.20% 105 38059 0.20% 106 38469 0.21% 107 40772 0.22% 108 44676 0.24% 109 44000 0.23% 110 45021 0.24% 111 47005 0.25% 112 48082 0.26% 113 48546 0.26% 114 53642 0.29% 115 55929 0.30% 116 56886 0.30% 117 56020 0.30% 118 58487 0.31% 119 56732 0.30% 120 58924 0.31% 121 63173 0.34% 122 71314 0.38% 123 70076 0.37% 124 71076 0.38% 125 73746 0.39% 126 71499 0.38% 127 73043 0.39% 128 72514 0.39% 129 75269 0.40% 130 71906 0.38% 131 77144 0.41% 132 79937 0.43% 133 78674 0.42% 134 83457 0.45% 135 82892 0.44% 136 87130 0.47% 137 88215 0.47% 138 95427 0.51% 139 99303 0.53% 140 102447 0.55% 141 116984 0.62% 142 117045 0.62% 143 125661 0.67% 144 142075 0.76% 145 170507 0.91% 146 194150 1.04% 147 250215 1.34% 148 361585 1.93% 149 707779 3.78% 150 4257687 22.73% 151 9375904 50.05% 18734942 reads passed initial QC criterion=sequence-density sequence-density=0.58 sequence-density-rank=1 fanout-score=5.02 fanout-score-rank=16 prefix-density=1.60 prefix-fanout=1.8 sequence=ATTTAGCCTTGGACGGAGTCTACCGCCCGATTTGGGCTGCATTCCCAAACAACCCGACTCGTTGACGGCGCCTCGTGGGGCGACAGGGTCCGGGCCGGACGGGGCTCTCACCCTCCCAGGCGCCCCTTTCCAGGGGACTTGGGCCCGGTCCGTCGCTGAGGACGCCTCTCCAGACTACAATTCGGACGGCACGGCCGCCCGATTCTCAAGCTGGGCTGCTCCCGGTTCGCTCGCCGTTACTAGGGGAATCCTTGTAAGTTTCTTCTCCTCCGCTTATTTATATGCTTAAACTCAGCGGGTAGTCCCGCCTGACC criterion=fanout-score sequence-density=0.01 sequence-density-rank=37 fanout-score=58.92 fanout-score-rank=1 prefix-density=0.14 prefix-fanout=5.6 sequence=ATAAAAAAAAGGGGGGGTAAGGACCCGCTAAGCTCCTACTTTTTCATGTTTCCAATCCGATCCCTCCGATTACTATAGAGATGAACCCAATCCAGAATATGAACCATAAAAGAAAACACCTACTAAACCAATCACAAGAATACCAGTTACCGTACCTATCAGCCAAAGAGGAATTCTTCCAGTAGTATCGGCCATTTCCCCTACTTTCCTCCACATTTTATCAAGTGGTCATGCTAGAGACAAAAACAGTCATGGATAGTTATGTTATAAGGATGGTATCCTTCCAAATGGGATAAGAGAGTTCTTACTACTCTCTTCTTTTCTCTCAATTAAAGAAGTAATTGGAAAACAAAACAGCAAGTACAAAAATGAGTAATAAACCCCAGTATAGACTGGTACGATTCAATTCAACATTTTGTTCATTCGGGTTTGATTGTGTCATAGTTCTATAGTTGGAATTTAGTTTATCGTTGGATGAACTGCATTGCTGATATTGATCCCAAGAAAAAAA criterion=sequence-density sequence-density=0.34 sequence-density-rank=1 fanout-score=9.65 fanout-score-rank=8 prefix-density=2.52 prefix-fanout=1.3 sequence=TGGTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGTTAACGAACGAGACCTCAGCCTGCTAACTAGCTATGCGGAGCCATCCCTCCGCAGCTAGCTTCTTAGAGGGACTATCGCCGTTTAGGCGACGGAAGTTTGAGGCAATAACAGGTCTGTGATGCCCTTAGATGTTCTGGGCCGCACGCGCGCTACACTGATGTATTCAACGAGTATATAGCCTTGGCCGACAGGCCCGGGTAATCTTGGGAAATTTCATCGTGATGGGGATAGATCATTGCAATTGTTGGTCTTCAACGAGGAATGCCTAGTAAGCGCGAGTCATCAGCTCGCGTTGACTACGTCCCTGCCCTTTGTACACACC criterion=fanout-score sequence-density=0.01 sequence-density-rank=39 fanout-score=87.00 fanout-score-rank=1 prefix-density=0.87 prefix-fanout=1.1 sequence=AGAAGGGGTGCCCCCTCACAAAAGGGGGTCGCAGTGACCAGGCCCGGGCGACTGTTTACCAAAAACACAGGTCTCCGCAAAGTCGTAAGACCATGTATGGGGGCTGACGCCTGCCCAGTGCCGGAAGGTCAAGGAAGTTGGTGAACTGATGACAGGGAAGCCGGCGACCGAAGCCCCGGTGAACGGCGGCCGTAAC SRR6941593 testing PE reads STAR mapping to Ensembl genome Started job on | Dec 06 12:33:22 Started mapping on | Dec 06 12:33:23 Finished on | Dec 06 12:35:38 Mapping speed, Million of reads per hour | 499.60 Number of input reads | 18734942 Average input read length | 290 UNIQUE READS: Uniquely mapped reads number | 9643126 Uniquely mapped reads % | 51.47% Average mapped length | 293.84 Number of splices: Total | 1518070 Number of splices: Annotated (sjdb) | 1348354 Number of splices: GT/AG | 1431510 Number of splices: GC/AG | 17689 Number of splices: AT/AC | 5441 Number of splices: Non-canonical | 63430 Mismatch rate per base, % | 0.15% Deletion rate per base | 0.01% Deletion average length | 1.52 Insertion rate per base | 0.01% Insertion average length | 1.74 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 7176507 % of reads mapped to multiple loci | 38.31% Number of reads mapped to too many loci | 219277 % of reads mapped to too many loci | 1.17% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 3.45% % of reads unmapped: other | 5.60% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 1920133 1920133 1920133 N_multimapping 7176507 7176507 7176507 N_noFeature 5204234 9371807 5314698 N_ambiguous 315698 5248 158632 UnstrandedReadsAssigned:4123194 PositiveStrandReadsAssigned:266071 NegativeStrandReadsAssigned:4169796 Dataset is classified negative stranded MeadianReadLen=151 20thPercentileLength=148 echo kmer=143 SRR6941593 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,972 [index] number of k-mers: 66,720,672 [index] number of equivalence classes: 111,837 [quant] running in paired-end mode [quant] will process pair 1: SRR6941593-trimmed-pair1.fastq SRR6941593-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 18,734,942 reads, 7,975,615 reads pseudoaligned [quant] estimated average fragment length: 211.275 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 950 rounds 52973 SRR6941593.ke.tsv 35125 SRR6941593.se.tsv 88098 total ==> SRR6941593.ke.tsv <== target_id length eff_length est_counts tpm PNS24245 936 726.115 0 0 PNS24247 1044 833.725 7.73003 0.829845 PNS24249 1928 1717.72 7.8099 0.40694 PNS24246 1044 833.725 7.73003 0.829845 PNS24248 1044 833.725 7.73003 0.829845 PNS24244 1471 1260.72 0 0 PNS24243 293 114.743 0 0 KQK14069 1603 1392.72 810.564 52.0907 KQK14071 474 271.941 22.9293 7.54664 ==> SRR6941593.se.tsv <== BRADI_1g14170v3 995 BRADI_1g53295v3 5 BRADI_1g59795v3 28 BRADI_1g07683v3 0 BRADI_1g00485v3 0 BRADI_1g20270v3 18 BRADI_1g74790v3 13 BRADI_1g09890v3 0 BRADI_1g77505v3 17 BRADI_1g48960v3 0 SRR6941593 completed mapping pipeline successfully