Starting /dee2/code/volunteer_pipeline.sh SRR6941596 current disk space = 1551262195712 free memory = 1603991284 SRR6941596 SRAfilesize b473a586c5e7b72ba9bba0175c2184af SRR6941596.sra SRR6941596.sra file validated SRR6941596 is paired end SRR6941596 is conventional basespace SRR6941596 read1 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR6941596_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.306 34.0 33.0 34.0 31.0 34.0 2 32.992 34.0 33.0 34.0 31.0 34.0 3 33.1365 34.0 33.0 34.0 32.0 34.0 4 33.3755 34.0 33.0 34.0 33.0 34.0 5 33.44675 34.0 33.0 34.0 33.0 34.0 6 37.2805 38.0 38.0 38.0 36.0 38.0 7 37.553 38.0 38.0 38.0 37.0 38.0 8 37.64075 38.0 38.0 38.0 38.0 38.0 9 37.6325 38.0 38.0 38.0 38.0 38.0 10-14 37.63865 38.0 38.0 38.0 38.0 38.0 15-19 37.65045 38.0 38.0 38.0 38.0 38.0 20-24 37.592650000000006 38.0 38.0 38.0 38.0 38.0 25-29 37.59605 38.0 38.0 38.0 38.0 38.0 30-34 37.5235 38.0 38.0 38.0 38.0 38.0 35-39 37.53745 38.0 38.0 38.0 38.0 38.0 40-44 37.55865 38.0 38.0 38.0 38.0 38.0 45-49 37.46595 38.0 38.0 38.0 38.0 38.0 50-54 37.55735 38.0 38.0 38.0 38.0 38.0 55-59 37.51465 38.0 38.0 38.0 38.0 38.0 60-64 37.477349999999994 38.0 38.0 38.0 38.0 38.0 65-69 37.4416 38.0 38.0 38.0 37.6 38.0 70-74 37.34665 38.0 38.0 38.0 37.0 38.0 75-79 37.36805 38.0 38.0 38.0 37.4 38.0 80-84 37.38119999999999 38.0 38.0 38.0 37.0 38.0 85-89 37.27675 38.0 38.0 38.0 37.0 38.0 90-94 37.25935 38.0 38.0 38.0 37.0 38.0 95-99 37.2556 38.0 38.0 38.0 36.8 38.0 100-104 37.10545 38.0 38.0 38.0 36.2 38.0 105-109 37.01285 38.0 38.0 38.0 35.6 38.0 110-114 36.75345 38.0 38.0 38.0 35.2 38.0 115-119 36.711349999999996 38.0 38.0 38.0 34.8 38.0 120-124 36.8243 38.0 38.0 38.0 35.0 38.0 125-129 36.68175000000001 38.0 38.0 38.0 35.0 38.0 130-134 36.5173 38.0 38.0 38.0 34.4 38.0 135-139 36.375099999999996 38.0 38.0 38.0 34.0 38.0 140-144 36.282 38.0 38.0 38.0 33.6 38.0 145-149 35.9712 38.0 38.0 38.0 33.0 38.0 150-151 32.344625 35.5 33.0 38.0 17.0 38.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 12 1.0 13 0.0 14 0.0 15 1.0 16 0.0 17 0.0 18 3.0 19 0.0 20 2.0 21 0.0 22 0.0 23 2.0 24 3.0 25 7.0 26 9.0 27 13.0 28 12.0 29 22.0 30 25.0 31 27.0 32 47.0 33 53.0 34 96.0 35 164.0 36 340.0 37 3173.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 46.66132478632478 14.23611111111111 7.532051282051282 31.570512820512818 2 24.60615153788447 15.95398849712428 30.182545636409102 29.257314328582147 3 20.8 24.45 26.474999999999998 28.275 4 24.6 31.75 20.8 22.85 5 21.675 37.375 22.85 18.099999999999998 6 18.575 37.375 23.375 20.674999999999997 7 14.774999999999999 27.575 39.525 18.125 8 16.75 26.650000000000002 30.099999999999998 26.5 9 17.25 24.125 31.574999999999996 27.05 10-14 21.195 31.685000000000002 23.34 23.78 15-19 21.36 28.87 26.195 23.575 20-24 20.080000000000002 30.2 25.695 24.025 25-29 21.875 29.325000000000003 25.55 23.25 30-34 21.82 31.1 24.785 22.295 35-39 21.2 29.59 26.26 22.95 40-44 20.095 28.660000000000004 26.66 24.585 45-49 20.754150830166033 29.68093618723745 26.79535907181436 22.769553910782157 50-54 21.255 28.720000000000002 25.7 24.325 55-59 20.995 29.395 25.900000000000002 23.71 60-64 20.155 28.93 26.63 24.285 65-69 20.78 29.17 25.185000000000002 24.865000000000002 70-74 21.63 28.884999999999998 24.735 24.75 75-79 21.17 29.020000000000003 25.91 23.9 80-84 22.61 28.865000000000002 25.115 23.41 85-89 21.495 27.705000000000002 26.884999999999998 23.915 90-94 20.885 29.385 24.9 24.83 95-99 20.315 29.544999999999998 25.174999999999997 24.965 100-104 21.048419367747098 29.761904761904763 24.344737895158065 24.844937975190078 105-109 21.805 28.96 25.5 23.735 110-114 21.216229788088782 29.07502259716782 25.424324595761778 24.28442301898162 115-119 21.629072681704262 29.91979949874687 23.984962406015036 24.466165413533837 120-124 21.305 30.665 21.945 26.085 125-129 21.735 30.320000000000004 24.0 23.945 130-134 22.63 30.490000000000002 22.675 24.205 135-139 22.400000000000002 30.080000000000002 23.31 24.21 140-144 22.68 29.17 23.995 24.154999999999998 145-149 22.18 29.060000000000002 24.055 24.705 150-151 20.4125 30.075000000000003 23.0 26.5125 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.5 6 0.5 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 1.0 19 2.0 20 2.0 21 2.0 22 4.0 23 4.0 24 3.5 25 5.5 26 7.5 27 10.0 28 14.0 29 15.5 30 22.5 31 25.5 32 20.5 33 26.5 34 35.5 35 42.0 36 87.0 37 192.5 38 219.5 39 203.0 40 241.5 41 254.0 42 214.0 43 210.0 44 226.5 45 204.5 46 165.0 47 132.0 48 128.0 49 110.5 50 90.0 51 77.5 52 67.5 53 78.5 54 107.0 55 124.0 56 123.5 57 98.5 58 77.0 59 66.5 60 56.5 61 45.5 62 26.0 63 25.5 64 26.5 65 18.5 66 11.5 67 8.0 68 6.0 69 5.0 70 8.0 71 4.5 72 1.5 73 2.0 74 2.5 75 4.0 76 2.5 77 1.5 78 1.0 79 0.0 80 0.0 81 0.5 82 0.5 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content warn #Base N-Count 1 6.4 2 0.025 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.02 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.04 105-109 0.0 110-114 0.43 115-119 0.25 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 69.75 #Duplication Level Percentage of deduplicated Percentage of total 1 84.6594982078853 59.050000000000004 2 8.566308243727597 11.95 3 2.3297491039426523 4.875 4 1.0752688172043012 3.0 5 0.7885304659498209 2.75 6 0.6451612903225806 2.7 7 0.39426523297491045 1.925 8 0.3225806451612903 1.7999999999999998 9 0.43010752688172044 2.7 >10 0.7526881720430108 7.95 >50 0.035842293906810034 1.3 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCATTAAT 52 1.3 No Hit GGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTACGTT 30 0.75 No Hit GCCAAAATAACCATGAGCAGCCACAATATTATAAGTCTCTTCCTCTTGAC 26 0.65 No Hit GCGGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTACG 20 0.5 No Hit GCGGAAAAACCTTATCCATTAATAGCGGGAACTTCAAGAGCAGCTAGATC 20 0.5 No Hit GTTGAAACTAGCATATTGGAAGATTAATCGGCCAAAATAACCATGAGCAG 19 0.475 No Hit GGCCAAAATAACCATGAGCAGCCACAATATTATAAGTCTCTTCCTCTTGA 17 0.42500000000000004 No Hit GCTAGTTATCCAGTTGCAGAAGCGACCCCACAGGCTTGTACTTTCGCGTC 15 0.375 No Hit GGAAAAACCTTATCCATTAATAGCGGGAACTTCAAGAGCAGCTAGATCTA 14 0.35000000000000003 No Hit GTGCTCTGCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTCCTA 14 0.35000000000000003 No Hit CTTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCAT 14 0.35000000000000003 No Hit ATAACAAGCTACACCAAGTAAGAAGTGTAGAACAATTAGCTCATAAGGAC 14 0.35000000000000003 No Hit GTACAAGGCCCGGGAACGGATTCACCGCCGTATGGCTGACCGGCGATTAC 13 0.325 No Hit CTCATAAGGACCACCATTGTATAACCATTCATCAACGGATGCAGCTTCCC 13 0.325 No Hit CATATAACAAGCTACACCAAGTAAGAAGTGTAGAACAATTAGCTCATAAG 12 0.3 No Hit GGTCAATAAGGTAGGGATCATCAAAACACCGAACCATCCGATGTAAAGAC 12 0.3 No Hit GGTAAATCAAGAAAACAGCAGTCGCAGCTGCAACAGGAGCTGAATATGCA 12 0.3 No Hit TTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCATT 11 0.27499999999999997 No Hit CCAGATTCCTACTACAGGCCAAGCAGCCAAGAAGAAGTGTAAAGAACGAG 11 0.27499999999999997 No Hit GCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTCCTAAAGGCAT 11 0.27499999999999997 No Hit GATGATATCAGCCCAAGTATTAATAACGCGACCTTGACTATCAACTACAG 10 0.25 No Hit GCATAAGGATGTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAAAGTA 10 0.25 No Hit CGGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTACGT 9 0.22499999999999998 No Hit AAAAATTCTTATATGTTAGCGGAAAAACCTTATCCATTAATAGCGGGAAC 9 0.22499999999999998 No Hit CGCAGAAGTAGGAATAATAGCACCAGAGATAATATTGTTTCCATAAAGTA 9 0.22499999999999998 No Hit CCGCAGAAGTAGGAATAATAGCACCAGAGATAATATTGTTTCCATAAAGT 9 0.22499999999999998 No Hit GCCGAAAACACCAGCTACACCTAACATGTGAAATGGATGCATAAGGATGT 9 0.22499999999999998 No Hit ATCAGAGAAGCTTCCTTGACCAATAGGGTAAATCAAGAAAACAGCAGTCG 9 0.22499999999999998 No Hit GGATGCATAAGGATGTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAA 9 0.22499999999999998 No Hit GCGACCTTGACTATCAACTACAGATTGGTTGAAATTGAATCCATTTAGGT 9 0.22499999999999998 No Hit TTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCATTAATA 9 0.22499999999999998 No Hit CTAACATGTGAAATGGATGCATAAGGATGTTGTGCTCTGCCTGGAATACA 9 0.22499999999999998 No Hit GTTCGCTGGAGTAGGCTGCGAGGCCCAAGCGGCCAAGGAGTGCAGCGGCC 9 0.22499999999999998 No Hit GCTCTGCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTCCTAAA 9 0.22499999999999998 No Hit GGAGGGGCTGCGATGAAGGCGATAATAAATACAGAAGTTGCGGTCAATAA 8 0.2 No Hit GGAAGATTAATCGGCCAAAATAACCATGAGCAGCCACAATATTATAAGTC 8 0.2 No Hit GTCGCAGCTGCAACAGGAGCTGAATATGCAACAGCAATCCAAGGGCGCAT 8 0.2 No Hit ATAAGGACCACCATTGTATAACCATTCATCAACGGATGCAGCTTCCCAAA 8 0.2 No Hit CCATATAACAAGCTACACCAAGTAAGAAGTGTAGAACAATTAGCTCATAA 8 0.2 No Hit TTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCATTA 8 0.2 No Hit GCTGCGATGAAGGCGATAATAAATACAGAAGTTGCGGTCAATAAGGTAGG 8 0.2 No Hit CGGAAAAACCTTATCCATTAATAGCGGGAACTTCAAGAGCAGCTAGATCT 8 0.2 No Hit CATAAGGATGTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAAAGTAC 8 0.2 No Hit GCACGGTTGATGATATCAGCCCAAGTATTAATAACGCGACCTTGACTATC 7 0.17500000000000002 No Hit AGAGAAGCTTCCTTGACCAATAGGGTAAATCAAGAAAACAGCAGTCGCAG 7 0.17500000000000002 No Hit GTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTC 7 0.17500000000000002 No Hit GGTTGATGATATCAGCCCAAGTATTAATAACGCGACCTTGACTATCAACT 7 0.17500000000000002 No Hit CAAGTATTAATAACGCGACCTTGACTATCAACTACAGATTGGTTGAAATT 7 0.17500000000000002 No Hit GGGATCATCAAAACACCGAACCATCCGATGTAAAGACGATTTTCAGTGCT 7 0.17500000000000002 No Hit GCTACACCTAACATGTGAAATGGATGCATAAGGATGTTGTGCTCTGCCTG 7 0.17500000000000002 No Hit CCAAAATAACCATGAGCAGCCACAATATTATAAGTCTCTTCCTCTTGACC 7 0.17500000000000002 No Hit CTCATTAGCAGATTCATTTTCAGTAGTTTCCCTGATCAAACTAGAGGTTA 7 0.17500000000000002 No Hit GTGAAATGGATGCATAAGGATGTTGTGCTCTGCCTGGAATACAATCATAA 7 0.17500000000000002 No Hit GCCAAGAAGAAGTGTAAAGAACGAGAGTTGTTGAAACTAGCATATTGGAA 7 0.17500000000000002 No Hit AAGTATTAATAACGCGACCTTGACTATCAACTACAGATTGGTTGAAATTG 6 0.15 No Hit GCGGTCAATAAGGTAGGGATCATCAAAACACCGAACCATCCGATGTAAAG 6 0.15 No Hit GGCGCATACCCAAACGGAAACTAAGTTCCCACTCACGACCCATATAACAA 6 0.15 No Hit ATCCGATTGCCGCAGAAGTAGGAATAATAGCACCAGAGATAATATTGTTT 6 0.15 No Hit GGGAAGTTGTGAGCATTACGTTCGTGCATTACTTCCATACCAAGATTAGC 6 0.15 No Hit CCCAAGTATTAATAACGCGACCTTGACTATCAACTACAGATTGGTTGAAA 6 0.15 No Hit GGATCATCAAAACACCGAACCATCCGATGTAAAGACGATTTTCAGTGCTA 6 0.15 No Hit GTAAAGAACGAGAGTTGTTGAAACTAGCATATTGGAAGATTAATCGGCCA 6 0.15 No Hit CGCATACCCAAACGGAAACTAAGTTCCCACTCACGACCCATATAACAAGC 6 0.15 No Hit CCACAATATTATAAGTCTCTTCCTCTTGACCAAATTTGTAACCCTCATTA 6 0.15 No Hit GGCCAAGCAGCCAAGAAGAAGTGTAAAGAACGAGAGTTGTTGAAACTAGC 6 0.15 No Hit GCTTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCA 6 0.15 No Hit AGCGGAAAAACCTTATCCATTAATAGCGGGAACTTCAAGAGCAGCTAGAT 6 0.15 No Hit CTTCACTCCAGTCGCAAGCCTAGCCTTAGGCATCCCCCTCCTTACGGTTA 6 0.15 No Hit CTCTGCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTCCTAAAG 6 0.15 No Hit GGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTACGTTC 6 0.15 No Hit GCCAAGCAGCCAAGAAGAAGTGTAAAGAACGAGAGTTGTTGAAACTAGCA 6 0.15 No Hit CTCGCGAATACCATCAATATCTACTGGAGGGGCTGCGATGAAGGCGATAA 6 0.15 No Hit ACCAGCTACACCTAACATGTGAAATGGATGCATAAGGATGTTGTGCTCTG 5 0.125 No Hit GCCCGACTCCCCAACCTAGGGTAGCTAAGTGTGGAAGTAAAATCAACCCT 5 0.125 No Hit GTATTAATAACGCGACCTTGACTATCAACTACAGATTGGTTGAAATTGAA 5 0.125 No Hit CTGCGATGAAGGCGATAATAAATACAGAAGTTGCGGTCAATAAGGTAGGG 5 0.125 No Hit CCTCAGCCTACGGGGTATTAGCAACCGTTTCCAGTTGTTGTTCCCCTCCC 5 0.125 No Hit CAAGAAAACAGCAGTCGCAGCTGCAACAGGAGCTGAATATGCAACAGCAA 5 0.125 No Hit TTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCATTAATAG 5 0.125 No Hit GCACTGAATAGGGAACCGCCGAAAACACCAGCTACACCTAACATGTGAAA 5 0.125 No Hit GCATATTGGAAGATTAATCGGCCAAAATAACCATGAGCAGCCACAATATT 5 0.125 No Hit CCCTCATTAGCAGATTCATTTTCAGTAGTTTCCCTGATCAAACTAGAGGT 5 0.125 No Hit CACTCACGACCCATATAACAAGCTACACCAAGTAAGAAGTGTAGAACAAT 5 0.125 No Hit CATCAGAGAAGCTTCCTTGACCAATAGGGTAAATCAAGAAAACAGCAGTC 5 0.125 No Hit CCAAGTATTAATAACGCGACCTTGACTATCAACTACAGATTGGTTGAAAT 5 0.125 No Hit CCACTCACGACCCATATAACAAGCTACACCAAGTAAGAAGTGTAGAACAA 5 0.125 No Hit ATATAACAAGCTACACCAAGTAAGAAGTGTAGAACAATTAGCTCATAAGG 5 0.125 No Hit GAGCTGAATATGCAACAGCAATCCAAGGGCGCATACCCAAACGGAAACTA 5 0.125 No Hit GATGCATAAGGATGTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAAA 5 0.125 No Hit GAACAATTAGCTCATAAGGACCACCATTGTATAACCATTCATCAACGGAT 5 0.125 No Hit GTGCGACGTGGGGCTGGATCTCAGTGGATCGTGGCAGCAAGGCCACTCTG 5 0.125 No Hit GTTGAAATTGAATCCATTTAGGTTGAACGCCATAGTACTAATACCTAAAG 5 0.125 No Hit GTTTACGGCTAGGACTACTGGGGTCTCTAATCCCATTTGCTCCCCTAGCT 5 0.125 No Hit GGGGCTGCGATGAAGGCGATAATAAATACAGAAGTTGCGGTCAATAAGGT 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.025 0.0 0.0 0.0 0.0 68-69 0.037500000000000006 0.0 0.0 0.0 0.0 70-71 0.1125 0.0 0.0 0.0 0.0 72-73 0.16249999999999998 0.0 0.0 0.0 0.0 74-75 0.175 0.0 0.0 0.0 0.0 76-77 0.2 0.0 0.0 0.0 0.0 78-79 0.25 0.0 0.0 0.0 0.0 80-81 0.2875 0.0 0.0 0.0 0.0 82-83 0.35 0.0 0.0 0.0 0.0 84-85 0.5125 0.0 0.0 0.0 0.0 86-87 0.6499999999999999 0.0 0.0 0.0 0.0 88-89 0.8625 0.0 0.0 0.0 0.0 90-91 1.125 0.0 0.0 0.0 0.0 92-93 1.3250000000000002 0.0 0.0 0.0 0.0 94-95 1.5125 0.0 0.0 0.0 0.0 96-97 1.9 0.0 0.0 0.0 0.0 98-99 2.0999999999999996 0.0 0.0 0.0 0.0 100-101 2.3625 0.0 0.0 0.0 0.0 102-103 2.6500000000000004 0.0 0.0 0.0 0.0 104-105 3.1 0.0 0.0 0.0 0.0 106-107 3.6375 0.0 0.0 0.0 0.0 108-109 4.1875 0.0 0.0 0.0 0.0 110-111 4.824999999999999 0.0 0.0 0.0 0.0 112-113 5.5875 0.0 0.0 0.0 0.0 114-115 6.3375 0.0 0.0 0.0 0.0 116-117 6.9375 0.0 0.0 0.0 0.0 118-119 7.7 0.0 0.0 0.0 0.0 120-121 8.3875 0.0 0.0 0.0 0.0 122-123 9.4625 0.0 0.0 0.0 0.0 124-125 10.5875 0.0 0.0 0.0 0.0 126-127 11.7375 0.0 0.0 0.0 0.0 128-129 12.3625 0.0 0.0 0.0 0.0 130-131 13.225000000000001 0.0 0.0 0.0 0.0 132-133 14.0 0.0 0.0 0.0 0.0 134-135 14.7625 0.0 0.0 0.0 0.0 136-137 15.525 0.0 0.0 0.0 0.0 138-139 16.6625 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AACTCCA 45 0.009006281 48.266666 145 GTCTGAA 65 4.226478E-4 15.593847 140-144 TCTGAAC 75 0.0012506887 13.5146675 140-144 ACACGTC 85 0.00320638 11.9247055 135-139 CACACGT 85 0.00320638 11.9247055 135-139 TCGGAAG 90 0.004916005 11.262223 125-129 AGAGCAC 90 0.004916005 11.262223 130-134 ATCGGAA 90 0.004916005 11.262223 125-129 >>END_MODULE SRR6941596 read2 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR6941596_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 33.19125 34.0 33.0 34.0 33.0 34.0 2 33.28425 34.0 33.0 34.0 33.0 34.0 3 33.341 34.0 33.0 34.0 33.0 34.0 4 33.2305 34.0 33.0 34.0 33.0 34.0 5 33.32525 34.0 33.0 34.0 33.0 34.0 6 37.50825 38.0 38.0 38.0 38.0 38.0 7 37.48275 38.0 38.0 38.0 38.0 38.0 8 37.44925 38.0 38.0 38.0 38.0 38.0 9 37.42675 38.0 38.0 38.0 38.0 38.0 10-14 37.4117 38.0 38.0 38.0 38.0 38.0 15-19 37.43275 38.0 38.0 38.0 38.0 38.0 20-24 37.401149999999994 38.0 38.0 38.0 38.0 38.0 25-29 37.36805 38.0 38.0 38.0 38.0 38.0 30-34 37.352000000000004 38.0 38.0 38.0 38.0 38.0 35-39 37.39489999999999 38.0 38.0 38.0 38.0 38.0 40-44 37.33969999999999 38.0 38.0 38.0 38.0 38.0 45-49 37.367 38.0 38.0 38.0 38.0 38.0 50-54 37.2969 38.0 38.0 38.0 37.6 38.0 55-59 37.21505 38.0 38.0 38.0 37.0 38.0 60-64 37.137299999999996 38.0 38.0 38.0 37.0 38.0 65-69 37.192099999999996 38.0 38.0 38.0 37.2 38.0 70-74 37.138999999999996 38.0 38.0 38.0 37.0 38.0 75-79 37.11095 38.0 38.0 38.0 37.0 38.0 80-84 37.114 38.0 38.0 38.0 36.8 38.0 85-89 37.0466 38.0 38.0 38.0 36.8 38.0 90-94 36.99995 38.0 38.0 38.0 36.2 38.0 95-99 36.879749999999994 38.0 38.0 38.0 36.0 38.0 100-104 36.7784 38.0 38.0 38.0 35.2 38.0 105-109 36.58495 38.0 38.0 38.0 34.8 38.0 110-114 36.12385 38.0 38.0 38.0 33.4 38.0 115-119 36.095299999999995 38.0 38.0 38.0 33.6 38.0 120-124 36.190200000000004 38.0 38.0 38.0 33.6 38.0 125-129 36.1336 38.0 38.0 38.0 33.8 38.0 130-134 36.005399999999995 38.0 38.0 38.0 33.4 38.0 135-139 35.644349999999996 38.0 37.4 38.0 31.6 38.0 140-144 35.2201 38.0 36.0 38.0 31.0 38.0 145-149 34.21785 38.0 35.8 38.0 26.6 38.0 150-151 28.725 34.5 18.5 38.0 2.0 38.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 2.0 4 3.0 5 1.0 6 2.0 7 0.0 8 0.0 9 2.0 10 2.0 11 1.0 12 1.0 13 0.0 14 1.0 15 5.0 16 2.0 17 3.0 18 4.0 19 2.0 20 3.0 21 4.0 22 5.0 23 8.0 24 8.0 25 7.0 26 10.0 27 24.0 28 16.0 29 26.0 30 25.0 31 35.0 32 39.0 33 68.0 34 109.0 35 198.0 36 460.0 37 2921.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.800000000000004 19.900000000000002 12.225 23.075000000000003 2 28.275 18.775 34.125 18.825 3 20.1 23.025000000000002 37.7 19.175 4 23.599999999999998 30.525000000000002 26.325 19.55 5 27.175 32.7 22.85 17.275 6 22.525000000000002 33.225 25.825 18.425 7 18.025 18.55 42.55 20.875 8 20.875 22.35 29.849999999999998 26.924999999999997 9 22.650000000000002 21.925 31.424999999999997 24.0 10-14 24.845 25.005 29.13 21.02 15-19 24.12 24.72 30.435000000000002 20.724999999999998 20-24 24.87 24.490000000000002 30.5 20.14 25-29 24.52 25.314999999999998 28.655 21.51 30-34 25.09 25.145 29.235 20.53 35-39 24.13 25.5 28.915000000000003 21.455 40-44 24.2 26.14 28.9 20.76 45-49 24.18 26.375 28.994999999999997 20.45 50-54 24.42 25.865 28.93 20.785 55-59 23.635 26.345000000000002 28.470000000000002 21.55 60-64 24.2 24.955 29.830000000000002 21.015 65-69 24.8 25.685000000000002 28.29 21.224999999999998 70-74 24.59 25.619999999999997 29.04 20.75 75-79 24.85 24.965 29.160000000000004 21.025 80-84 25.215 24.525 30.005 20.255000000000003 85-89 24.66 25.705 28.610000000000003 21.025 90-94 24.15 25.595000000000002 28.4 21.855 95-99 25.005 24.87 29.26 20.865000000000002 100-104 24.995 25.855 28.939999999999998 20.21 105-109 25.845169033806766 24.054810962192438 29.66593318663733 20.43408681736347 110-114 24.64492898579716 25.465093018603717 29.15583116623325 20.734146829365873 115-119 25.874999999999996 25.740000000000002 28.599999999999998 19.785 120-124 25.805 27.22 26.400000000000002 20.575 125-129 25.97 26.5 27.005000000000003 20.525 130-134 26.14 26.525 27.584999999999997 19.75 135-139 26.889999999999997 25.779999999999998 27.685 19.645000000000003 140-144 27.755551110222044 25.745149029805965 28.040608121624327 18.458691738347667 145-149 27.600180099054477 25.914252839061486 27.590174596027815 18.89539246585622 150-151 28.119134025779 25.703916906519837 27.380803403829308 18.796145663871854 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.5 2 0.5 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.5 17 0.5 18 0.5 19 1.5 20 2.5 21 2.0 22 1.5 23 2.5 24 2.5 25 8.0 26 12.0 27 11.5 28 11.5 29 18.5 30 24.0 31 24.0 32 29.5 33 38.5 34 55.5 35 79.0 36 104.5 37 129.5 38 156.5 39 182.0 40 213.5 41 219.0 42 212.0 43 235.5 44 255.5 45 210.5 46 166.0 47 141.0 48 116.5 49 106.5 50 83.0 51 80.0 52 79.0 53 75.5 54 95.5 55 120.0 56 112.0 57 89.0 58 79.5 59 84.5 60 70.5 61 55.5 62 53.0 63 32.5 64 18.5 65 15.0 66 11.5 67 11.5 68 10.5 69 6.0 70 4.0 71 9.0 72 9.5 73 4.0 74 3.5 75 3.5 76 3.0 77 3.5 78 2.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.02 110-114 0.02 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.02 145-149 0.055 150-151 0.11249999999999999 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 67.60000000000001 #Duplication Level Percentage of deduplicated Percentage of total 1 81.02810650887574 54.775 2 9.985207100591715 13.5 3 3.6242603550295858 7.35 4 1.7011834319526626 4.6 5 0.9615384615384616 3.25 6 0.9615384615384616 3.9 7 0.2958579881656805 1.4000000000000001 8 0.33284023668639057 1.7999999999999998 9 0.22189349112426035 1.35 >10 0.8875739644970414 8.075000000000001 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTTCGATCCTGGCTCAGGATGAACGCTGGCGGCATGCTTAACACATGCAA 27 0.675 No Hit ATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAA 20 0.5 No Hit GCTTGTTATATGGGTCGTGAGTGGGAACTTAGTTTCCGTTTGGGTATGCG 17 0.42500000000000004 No Hit CTATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAA 16 0.4 No Hit GTGGGAACTTAGTTTCCGTTTGGGTATGCGCCCTTGGATTGCTGTTGCAT 16 0.4 No Hit GCACAACATCCTTATGCATCCATTTCACATGTTAGGTGTAGCTGGTGTTT 16 0.4 No Hit GGAAACTACTGAAAATGAATCTGCTAATGAGGGTTACAAATTTGGTCAAG 14 0.35000000000000003 No Hit GCTGCGACTGCTGTTTTCTTGATTTACCCTATTGGTCAAGGAAGCTTCTC 14 0.35000000000000003 No Hit GGTACTTTCAACTTTATGATTGTATTCCAGGCAGAGCACAACATCCTTAT 14 0.35000000000000003 No Hit ATCGGATTGCACTTTTACCCAATTTGGGAAGCTGCATCCGTTGATGAATG 13 0.325 No Hit CATCAACCGTGCTAATCTTGGTATGGAAGTAATGCACGAACGTAATGCTC 13 0.325 No Hit TCTATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGA 13 0.325 No Hit TTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAG 12 0.3 No Hit ATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCA 12 0.3 No Hit GGTCGCTTCTGCAACTGGATAACTAGCACTGAAAATCGTCTTTACATCGG 11 0.27499999999999997 No Hit TATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAA 11 0.27499999999999997 No Hit GCTAGTTTCAACAACTCTCGTTCTTTACACTTCTTCTTGGCTGCTTGGCC 11 0.27499999999999997 No Hit AGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGATCT 11 0.27499999999999997 No Hit ATTCAGTGCTATGCATGGTTCCTTGGTAACCTCTAGTTTGATCAGGGAAA 11 0.27499999999999997 No Hit GTTCAACCTAAATGGATTCAATTTCAACCAATCTGTAGTTGATAGTCAAG 11 0.27499999999999997 No Hit GTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGATCTT 10 0.25 No Hit ATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATA 10 0.25 No Hit ATGCGCCCTTGGATTGCTGTTGCATATTCAGCTCCTGTTGCAGCTGCGAC 10 0.25 No Hit GGAAGCTTCTCTGATGGTATGCCTTTAGGAATATCTGGTACTTTCAACTT 10 0.25 No Hit CCTACTTCTGCGGCAATCGGATTGCACTTTTACCCAATTTGGGAAGCTGC 9 0.22499999999999998 No Hit GCTAATTGTTCTACACTTCTTACTTGGTGTAGCTTGTTATATGGGTCGTG 9 0.22499999999999998 No Hit GGGAAACTACTGAAAATGAATCTGCTAATGAGGGTTACAAATTTGGTCAA 9 0.22499999999999998 No Hit ATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCTAAATGG 9 0.22499999999999998 No Hit GGGAACTTAGTTTCCGTTTGGGTATGCGCCCTTGGATTGCTGTTGCATAT 9 0.22499999999999998 No Hit GTCAAGGTCGCGTTATTAATACTTGGGCTGATATCATCAACCGTGCTAAT 9 0.22499999999999998 No Hit GGTGTTTTGATGATCCCTACCTTATTGACCGCAACTTCTGTATTTATTAT 8 0.2 No Hit CGTTGATGAATGGTTATACAATGGTGGTCCTTATGAGCTAATTGTTCTAC 8 0.2 No Hit CTTTACTTTATGGAAACAATATTATCTCTGGTGCTATTATTCCTACTTCT 8 0.2 No Hit ATTTGGTCAAGAGGAAGAGACTTATAATATTGTGGCTGCTCATGGTTATT 8 0.2 No Hit GTTTTGATGATCCCTACCTTATTGACCGCAACTTCTGTATTTATTATCGC 8 0.2 No Hit GGCTTGCGGTGGATACCTAGGCACCCAGAGACGAGGAAGGGCGTAGCAAG 8 0.2 No Hit TGTAGCTGGTGTTTTCGGCGGTTCCCTATTCAGTGCTATGCATGGTTCCT 8 0.2 No Hit CGGCAATCGGATTGCACTTTTACCCAATTTGGGAAGCTGCATCCGTTGAT 8 0.2 No Hit ATCTATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTG 8 0.2 No Hit TGGTAACCTCTAGTTTGATCAGGGAAACTACTGAAAATGAATCTGCTAAT 7 0.17500000000000002 No Hit ATATCATCAACCGTGCTAATCTTGGTATGGAAGTAATGCACGAACGTAAT 7 0.17500000000000002 No Hit CATGACTGCAATTTTAGAGAGACGCGAAAGTACAAGCCTGTGGGGTCGCT 7 0.17500000000000002 No Hit ATTATCTCTGGTGCTATTATTCCTACTTCTGCGGCAATCGGATTGCACTT 7 0.17500000000000002 No Hit GTTGCATATTCAGCTCCTGTTGCAGCTGCGACTGCTGTTTTCTTGATTTA 7 0.17500000000000002 No Hit GTTTCAACAACTCTCGTTCTTTACACTTCTTCTTGGCTGCTTGGCCTGTA 7 0.17500000000000002 No Hit TATCTATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTT 7 0.17500000000000002 No Hit GGAAGTAATGCACGAACGTAATGCTCACAACTTCCCTCTAGATCTAGCTG 7 0.17500000000000002 No Hit AGCACAACATCCTTATGCATCCATTTCACATGTTAGGTGTAGCTGGTGTT 6 0.15 No Hit GGAAGAGACTTATAATATTGTGGCTGCTCATGGTTATTTTGGCCGATTAA 6 0.15 No Hit TTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAA 6 0.15 No Hit CTTTTACCCAATTTGGGAAGCTGCATCCGTTGATGAATGGTTATACAATG 6 0.15 No Hit GCGCCCTTGGATTGCTGTTGCATATTCAGCTCCTGTTGCAGCTGCGACTG 6 0.15 No Hit GGTAACCTCTAGTTTGATCAGGGAAACTACTGAAAATGAATCTGCTAATG 6 0.15 No Hit ATTAATACTTGGGCTGATATCATCAACCGTGCTAATCTTGGTATGGAAGT 6 0.15 No Hit GTCGTGAGTGGGAACTTAGTTTCCGTTTGGGTATGCGCCCTTGGATTGCT 6 0.15 No Hit CCTAAATGGATTCAATTTCAACCAATCTGTAGTTGATAGTCAAGGTCGCG 6 0.15 No Hit GGCTGCTTGGCCTGTAGTAGGAATCTGGTTCACTGCTTTAGGTATTAGTA 6 0.15 No Hit GGAATATCTGGTACTTTCAACTTTATGATTGTATTCCAGGCAGAGCACAA 6 0.15 No Hit GAACGCTGGCGGCATGCTTAACACATGCAAGTCGAACGGGAAGTGGTGTT 6 0.15 No Hit AATGGATTCAATTTCAACCAATCTGTAGTTGATAGTCAAGGTCGCGTTAT 6 0.15 No Hit GTTATTTTGGCCGATTAATCTTCCAATATGCTAGTTTCAACAACTCTCGT 6 0.15 No Hit GGTTATACAATGGTGGTCCTTATGAGCTAATTGTTCTACACTTCTTACTT 6 0.15 No Hit GCATGGTTCCTTGGTAACCTCTAGTTTGATCAGGGAAACTACTGAAAATG 6 0.15 No Hit GTTATACAATGGTGGTCCTTATGAGCTAATTGTTCTACACTTCTTACTTG 6 0.15 No Hit CCCTATTCAGTGCTATGCATGGTTCCTTGGTAACCTCTAGTTTGATCAGG 6 0.15 No Hit GACCAGGCCCGGGCGACTGTTTACCAAAAACACAGGTCTCCGCAAAGTCG 6 0.15 No Hit CGCGAAAGTACAAGCCTGTGGGGTCGCTTCTGCAACTGGATAACTAGCAC 6 0.15 No Hit TCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGA 6 0.15 No Hit GGTCAAGGAAGCTTCTCTGATGGTATGCCTTTAGGAATATCTGGTACTTT 6 0.15 No Hit CCTTACCAGGGCTTGACATGCCGCGAATCCTCTTGAAAGAGAGGGGTGCC 6 0.15 No Hit ATTATCTATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAAT 6 0.15 No Hit GTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGATCTTACCATGAC 6 0.15 No Hit TGATAGTCAAGGTCGCGTTATTAATACTTGGGCTGATATCATCAACCGTG 6 0.15 No Hit CAAGATCTTACCATGACTGCAATTTTAGAGAGACGCGAAAGTACAAGCCT 5 0.125 No Hit GTATGCGCCCTTGGATTGCTGTTGCATATTCAGCTCCTGTTGCAGCTGCG 5 0.125 No Hit TCTTAATTCAAGAGTTGTAAGGAGGGACTTATGTCACCACAAACAGAAAC 5 0.125 No Hit CCGCAACTTCTGTATTTATTATCGCCTTCATCGCAGCCCCTCCAGTAGAT 5 0.125 No Hit GTCCTTATGAGCTAATTGTTCTACACTTCTTACTTGGTGTAGCTTGTTAT 5 0.125 No Hit GTACTATGGCGTTCAACCTAAATGGATTCAATTTCAACCAATCTGTAGTT 5 0.125 No Hit CTGCGACTGCTGTTTTCTTGATTTACCCTATTGGTCAAGGAAGCTTCTCT 5 0.125 No Hit ATCGCCTTCATCGCAGCCCCTCCAGTAGATATTGATGGTATTCGCGAGCC 5 0.125 No Hit GTTCCTTGGTAACCTCTAGTTTGATCAGGGAAACTACTGAAAATGAATCT 5 0.125 No Hit GTGACCAGGCCCGGGCGACTGTTTACCAAAAACACAGGTCTCCGCAAAGT 5 0.125 No Hit GCAATTTGAATAAACCAAGATCTTACCATGACTGCAATTTTAGAGAGACG 5 0.125 No Hit AATCGGATTGCACTTTTACCCAATTTGGGAAGCTGCATCCGTTGATGAAT 5 0.125 No Hit ATTGCTGTTGCATATTCAGCTCCTGTTGCAGCTGCGACTGCTGTTTTCTT 5 0.125 No Hit GTTGATAGTCAAGGTCGCGTTATTAATACTTGGGCTGATATCATCAACCG 5 0.125 No Hit GGATGGTTCGGTGTTTTGATGATCCCTACCTTATTGACCGCAACTTCTGT 5 0.125 No Hit CAGTGACCAGGCCCGGGCGACTGTTTACCAAAAACACAGGTCTCCGCAAA 5 0.125 No Hit CACATGTTAGGTGTAGCTGGTGTTTTCGGCGGTTCCCTATTCAGTGCTAT 5 0.125 No Hit CGCTTCTGCAACTGGATAACTAGCACTGAAAATCGTCTTTACATCGGATG 5 0.125 No Hit GTTAGGTGTAGCTGGTGTTTTCGGCGGTTCCCTATTCAGTGCTATGCATG 5 0.125 No Hit GTGTTTTCGGCGGTTCCCTATTCAGTGCTATGCATGGTTCCTTGGTAACC 5 0.125 No Hit GGTAAGTTCCGACCCGCACGAAAGGCGTAACGATCTGGGCACTGTCTCGG 5 0.125 No Hit GTTTGATCAGGGAAACTACTGAAAATGAATCTGCTAATGAGGGTTACAAA 5 0.125 No Hit CTTCCAATATGCTAGTTTCAACAACTCTCGTTCTTTACACTTCTTCTTGG 5 0.125 No Hit TCCTACTTCTGCGGCAATCGGATTGCACTTTTACCCAATTTGGGAAGCTG 5 0.125 No Hit GATGATCCCTACCTTATTGACCGCAACTTCTGTATTTATTATCGCCTTCA 5 0.125 No Hit GTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGATCTTACCATGACTG 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.025 0.0 0.0 0.0 0.0 68-69 0.037500000000000006 0.0 0.0 0.0 0.0 70-71 0.1125 0.0 0.0 0.0 0.0 72-73 0.16249999999999998 0.0 0.0 0.0 0.0 74-75 0.175 0.0 0.0 0.0 0.0 76-77 0.2 0.0 0.0 0.0 0.0 78-79 0.25 0.0 0.0 0.0 0.0 80-81 0.2875 0.0 0.0 0.0 0.0 82-83 0.35 0.0 0.0 0.0 0.0 84-85 0.5125 0.0 0.0 0.0 0.0 86-87 0.6499999999999999 0.0 0.0 0.0 0.0 88-89 0.8625 0.0 0.0 0.0 0.0 90-91 1.0875 0.0 0.0 0.0 0.0 92-93 1.275 0.0 0.0 0.0 0.0 94-95 1.4875 0.0 0.0 0.0 0.0 96-97 1.875 0.0 0.0 0.0 0.0 98-99 2.075 0.0 0.0 0.0 0.0 100-101 2.3375 0.0 0.0 0.0 0.0 102-103 2.625 0.0 0.0 0.0 0.0 104-105 3.075 0.0 0.0 0.0 0.0 106-107 3.575 0.0 0.0 0.0 0.0 108-109 4.112500000000001 0.0 0.0 0.0 0.0 110-111 4.7875 0.0 0.0 0.0 0.0 112-113 5.5875 0.0 0.0 0.0 0.0 114-115 6.387499999999999 0.0 0.0 0.0 0.0 116-117 7.025 0.0 0.0 0.0 0.0 118-119 7.775 0.0 0.0 0.0 0.0 120-121 8.4625 0.0 0.0 0.0 0.0 122-123 9.5 0.0 0.0 0.0 0.0 124-125 10.5875 0.0 0.0 0.0 0.0 126-127 11.7125 0.0 0.0 0.0 0.0 128-129 12.399999999999999 0.0 0.0 0.0 0.0 130-131 13.3 0.0 0.0 0.0 0.0 132-133 14.075 0.0 0.0 0.0 0.0 134-135 14.837499999999999 0.0 0.0 0.0 0.0 136-137 15.6 0.0 0.0 0.0 0.0 138-139 16.6875 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TGTAGGG 65 4.1823133E-4 15.615384 140-144 GTAGGGA 65 4.1823133E-4 15.615384 140-144 GTCGTGT 80 0.0020131238 12.6875 135-139 CGTCGTG 80 0.0020131238 12.6875 135-139 AAGAGCG 90 0.0048656333 11.277777 130-134 TCGGAAG 90 0.0048656333 11.277777 125-129 AGAGCGT 90 0.0048656333 11.277777 130-134 ATCGGAA 90 0.0048656333 11.277777 125-129 >>END_MODULE Read 1367330 spots for SRR6941596.sra Written 1367330 spots for SRR6941596.sra Read 1367330 spots for SRR6941596.sra Written 1367330 spots for SRR6941596.sra Read 1367330 spots for SRR6941596.sra Written 1367330 spots for SRR6941596.sra Read 1367330 spots for SRR6941596.sra Written 1367330 spots for SRR6941596.sra Read 1367330 spots for SRR6941596.sra Written 1367330 spots for SRR6941596.sra Read 1367330 spots for SRR6941596.sra Written 1367330 spots for SRR6941596.sra Read 1367330 spots for SRR6941596.sra Written 1367330 spots for SRR6941596.sra Read 1367330 spots for SRR6941596.sra Written 1367330 spots for SRR6941596.sra Read 1367330 spots for SRR6941596.sra Written 1367330 spots for SRR6941596.sra Read 1367330 spots for SRR6941596.sra Written 1367330 spots for SRR6941596.sra Read 1367330 spots for SRR6941596.sra Written 1367330 spots for SRR6941596.sra Read 1367330 spots for SRR6941596.sra Written 1367330 spots for SRR6941596.sra Read 1367330 spots for SRR6941596.sra Written 1367330 spots for SRR6941596.sra Read 1367330 spots for SRR6941596.sra Written 1367330 spots for SRR6941596.sra Read 1367330 spots for SRR6941596.sra Written 1367330 spots for SRR6941596.sra Read 1367330 spots for SRR6941596.sra Written 1367330 spots for SRR6941596.sra Read 1367330 spots for SRR6941596.sra Written 1367330 spots for SRR6941596.sra Read 1367333 spots for SRR6941596.sra Written 1367333 spots for SRR6941596.sra Read 1367330 spots for SRR6941596.sra Written 1367330 spots for SRR6941596.sra Read 1367330 spots for SRR6941596.sra Written 1367330 spots for SRR6941596.sra SRR ids: ['SRR6941596.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_y8us97pb SRR6941596.sra spots: 27346603 blocks: [[1, 1367330], [1367331, 2734660], [2734661, 4101990], [4101991, 5469320], [5469321, 6836650], [6836651, 8203980], [8203981, 9571310], [9571311, 10938640], [10938641, 12305970], [12305971, 13673300], [13673301, 15040630], [15040631, 16407960], [16407961, 17775290], [17775291, 19142620], [19142621, 20509950], [20509951, 21877280], [21877281, 23244610], [23244611, 24611940], [24611941, 25979270], [25979271, 27346603]] SRR6941596 file size 9245166 SRR6941596 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR6941596 SRR6941596_1.fastq SRR6941596_2.fastq Input file: SRR6941596_1.fastq Paired file: SRR6941596_2.fastq trimmed: SRR6941596-trimmed-pair1.fastq, SRR6941596-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Fri Dec 6 12:36:11 2024 >> started Fri Dec 6 12:36:39 2024 >> done (27.665s) 27346603 read pairs processed; of these: 26446 ( 0.10%) short read pairs filtered out after trimming by size control 21324 ( 0.08%) empty read pairs filtered out after trimming by size control 27298833 (99.83%) read pairs available; of these: 14723333 (53.93%) trimmed read pairs available after processing 12575500 (46.07%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 4 0.00% 19 0 0.00% 20 0 0.00% 21 4 0.00% 22 5 0.00% 23 1 0.00% 24 7 0.00% 25 4 0.00% 26 5 0.00% 27 23 0.00% 28 8 0.00% 29 6 0.00% 30 7 0.00% 31 13 0.00% 32 9 0.00% 33 3 0.00% 34 12 0.00% 35 10 0.00% 36 8 0.00% 37 12 0.00% 38 28 0.00% 39 21 0.00% 40 23 0.00% 41 34 0.00% 42 29 0.00% 43 41 0.00% 44 44 0.00% 45 42 0.00% 46 55 0.00% 47 74 0.00% 48 87 0.00% 49 114 0.00% 50 143 0.00% 51 164 0.00% 52 201 0.00% 53 204 0.00% 54 260 0.00% 55 323 0.00% 56 328 0.00% 57 363 0.00% 58 458 0.00% 59 554 0.00% 60 635 0.00% 61 805 0.00% 62 1013 0.00% 63 1189 0.00% 64 1339 0.00% 65 1496 0.01% 66 1611 0.01% 67 1782 0.01% 68 2167 0.01% 69 2363 0.01% 70 2854 0.01% 71 3373 0.01% 72 4105 0.02% 73 4672 0.02% 74 5014 0.02% 75 5906 0.02% 76 6525 0.02% 77 7516 0.03% 78 8214 0.03% 79 9616 0.04% 80 10890 0.04% 81 12326 0.05% 82 13864 0.05% 83 15449 0.06% 84 18435 0.07% 85 21368 0.08% 86 22331 0.08% 87 25276 0.09% 88 29740 0.11% 89 29794 0.11% 90 34050 0.12% 91 34137 0.13% 92 39737 0.15% 93 40826 0.15% 94 44078 0.16% 95 50012 0.18% 96 47849 0.18% 97 51486 0.19% 98 54625 0.20% 99 57670 0.21% 100 59018 0.22% 101 63955 0.23% 102 66872 0.24% 103 65808 0.24% 104 72452 0.27% 105 75519 0.28% 106 75702 0.28% 107 81018 0.30% 108 86846 0.32% 109 85909 0.31% 110 89230 0.33% 111 92618 0.34% 112 96248 0.35% 113 94333 0.35% 114 108090 0.40% 115 111771 0.41% 116 112770 0.41% 117 112196 0.41% 118 111697 0.41% 119 109844 0.40% 120 111762 0.41% 121 119210 0.44% 122 139014 0.51% 123 134152 0.49% 124 136455 0.50% 125 141896 0.52% 126 132652 0.49% 127 138386 0.51% 128 136378 0.50% 129 144185 0.53% 130 132304 0.48% 131 142002 0.52% 132 152301 0.56% 133 140387 0.51% 134 152614 0.56% 135 146942 0.54% 136 151287 0.55% 137 151314 0.55% 138 165861 0.61% 139 170176 0.62% 140 172775 0.63% 141 201927 0.74% 142 194847 0.71% 143 212511 0.78% 144 229620 0.84% 145 274919 1.01% 146 306998 1.12% 147 373018 1.37% 148 545284 2.00% 149 978102 3.58% 150 5892289 21.58% 151 12575500 46.07% 27298833 reads passed initial QC criterion=sequence-density sequence-density=0.42 sequence-density-rank=1 fanout-score=2.43 fanout-score-rank=26 prefix-density=0.43 prefix-fanout=2.4 sequence=GTTACGGCCGCCGTTCACCGGGGCTTCGGTCGCCGGCTTCCCTGTCATCAGTTCACCAACTTCCTTGACCTTCCGGCACTGGGCAGGCGTCAGCCCCCATACATGGTCTTACGACTTTGCGGAGACCTGTGTTTTTGGTAAACAGTCGCCCGGGCCTGGTCACTG criterion=fanout-score sequence-density=0.01 sequence-density-rank=41 fanout-score=31.61 fanout-score-rank=1 prefix-density=0.05 prefix-fanout=5.8 sequence=AAAAAAAAAGGGGGGTAAGGACCCGCTAAGCTCCTACTTTTTCATGTTTCCAATCCGATCCCTCCGATTACTATAGAGATGAACCCAATCCAGAATATGAACCATAAAAGAAAACACCTACTAAACCAATCACAAGAATACCAGTTACCGTACCTATCAGCCAAAGAGGAATTCTTCCAGTAGTATCGGCCATTTCCCCTACTTTCCTCCACATTTTATCAAGTGGTCATGCTAGAGACAAAAACAGTCATGGATAGTTATGTTATAAGGATGGTATCCTTCCAAATGGGATAAGAGAGTTCTTACTACTCTCTTCTTTTCTCTCAATTAAAGAAGTAATTGGAAAACAAAACAGCAAGTACAAAAATGAGTAATAAACCCCAGTATAGACTGGTACGATTCAATTCAACATTTTGTTCATTCGGGTTTGATTGTGTCATAGTTCTATAGTTGGAATTTAGTTTATCGTTGGATGAACTGCATTGCTGATATTGATCCCAAGAAAAAAACA criterion=sequence-density sequence-density=0.35 sequence-density-rank=1 fanout-score=2.87 fanout-score-rank=26 prefix-density=1.01 prefix-fanout=1.0 sequence=TTGCGTAGTGGATCTGCTGGGGCCTATGCGAAAGCTGGGCCTCACGGATCCTAGAGCGGCAGGCACCGCGTGAGGCTG criterion=fanout-score sequence-density=0.07 sequence-density-rank=28 fanout-score=19.30 fanout-score-rank=1 prefix-density=1.32 prefix-fanout=1.0 sequence=CAAGTCGAACGTTGTTTTCGGGGAGCTGGGCAGAAGGAAAAGAGGCTCCTAGCTAAAGTTGTCTCGCCCTGCTTCAAAACTACAGGGCGCGCGCTACGGCTTTGACCTAACGGCCTCCGTTTGCTGGAATCGGAATAGTTGAGAACAAAGTGGCGAACG SRR6941596 testing PE reads STAR mapping to Ensembl genome Started job on | Dec 06 12:37:10 Started mapping on | Dec 06 12:37:10 Finished on | Dec 06 12:39:38 Mapping speed, Million of reads per hour | 664.03 Number of input reads | 27298833 Average input read length | 287 UNIQUE READS: Uniquely mapped reads number | 15478465 Uniquely mapped reads % | 56.70% Average mapped length | 290.68 Number of splices: Total | 3020751 Number of splices: Annotated (sjdb) | 2720409 Number of splices: GT/AG | 2873297 Number of splices: GC/AG | 35414 Number of splices: AT/AC | 9491 Number of splices: Non-canonical | 102549 Mismatch rate per base, % | 0.29% Deletion rate per base | 0.02% Deletion average length | 1.99 Insertion rate per base | 0.02% Insertion average length | 2.35 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 9823459 % of reads mapped to multiple loci | 35.98% Number of reads mapped to too many loci | 158214 % of reads mapped to too many loci | 0.58% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 4.14% % of reads unmapped: other | 2.60% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 2011516 2011516 2011516 N_multimapping 9823459 9823459 9823459 N_noFeature 6757849 14987988 6956500 N_ambiguous 535094 7972 249275 UnstrandedReadsAssigned:8185522 PositiveStrandReadsAssigned:482505 NegativeStrandReadsAssigned:8272690 Dataset is classified negative stranded MeadianReadLen=151 20thPercentileLength=142 echo kmer=137 SRR6941596 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,972 [index] number of k-mers: 66,720,672 [index] number of equivalence classes: 111,837 [quant] running in paired-end mode [quant] will process pair 1: SRR6941596-trimmed-pair1.fastq SRR6941596-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 27,298,833 reads, 14,106,331 reads pseudoaligned [quant] estimated average fragment length: 209.317 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,078 rounds 52973 SRR6941596.ke.tsv 35125 SRR6941596.se.tsv 88098 total ==> SRR6941596.ke.tsv <== target_id length eff_length est_counts tpm PNS24245 936 728.127 0 0 PNS24247 1044 835.683 4.65696 0.356564 PNS24249 1928 1719.68 11.2889 0.420031 PNS24246 1044 835.683 4.65696 0.356564 PNS24248 1044 835.683 4.65696 0.356564 PNS24244 1471 1262.68 21.7402 1.10165 PNS24243 293 120.745 0 0 KQK14069 1603 1394.68 654.737 30.0378 KQK14071 474 276.918 15.5841 3.60085 ==> SRR6941596.se.tsv <== BRADI_1g14170v3 798 BRADI_1g53295v3 38 BRADI_1g59795v3 11 BRADI_1g07683v3 0 BRADI_1g00485v3 0 BRADI_1g20270v3 53 BRADI_1g74790v3 19 BRADI_1g09890v3 0 BRADI_1g77505v3 56 BRADI_1g48960v3 0 SRR6941596 completed mapping pipeline successfully