Starting /dee2/code/volunteer_pipeline.sh SRR6941597 current disk space = 1551379476480 free memory = 1600880424 SRR6941597 SRAfilesize 0e4ee19ffb9d59353803db55a8e078fe SRR6941597.sra SRR6941597.sra file validated SRR6941597 is paired end SRR6941597 is conventional basespace SRR6941597 read1 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR6941597_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.378 34.0 33.0 34.0 30.0 34.0 2 32.908 34.0 33.0 34.0 31.0 34.0 3 33.0765 34.0 33.0 34.0 32.0 34.0 4 33.296 34.0 33.0 34.0 33.0 34.0 5 33.3505 34.0 33.0 34.0 33.0 34.0 6 37.199 38.0 38.0 38.0 36.0 38.0 7 37.5165 38.0 38.0 38.0 37.0 38.0 8 37.5355 38.0 38.0 38.0 38.0 38.0 9 37.592 38.0 38.0 38.0 38.0 38.0 10-14 37.58905 38.0 38.0 38.0 38.0 38.0 15-19 37.65215 38.0 38.0 38.0 38.0 38.0 20-24 37.59075 38.0 38.0 38.0 38.0 38.0 25-29 37.6104 38.0 38.0 38.0 38.0 38.0 30-34 37.50095 38.0 38.0 38.0 38.0 38.0 35-39 37.53625 38.0 38.0 38.0 38.0 38.0 40-44 37.49795 38.0 38.0 38.0 37.8 38.0 45-49 37.4491 38.0 38.0 38.0 37.8 38.0 50-54 37.4845 38.0 38.0 38.0 38.0 38.0 55-59 37.43295 38.0 38.0 38.0 37.6 38.0 60-64 37.402550000000005 38.0 38.0 38.0 37.6 38.0 65-69 37.363150000000005 38.0 38.0 38.0 37.0 38.0 70-74 37.2247 38.0 38.0 38.0 37.0 38.0 75-79 37.292049999999996 38.0 38.0 38.0 37.0 38.0 80-84 37.27185 38.0 38.0 38.0 37.0 38.0 85-89 37.2202 38.0 38.0 38.0 37.0 38.0 90-94 37.129200000000004 38.0 38.0 38.0 36.6 38.0 95-99 37.120999999999995 38.0 38.0 38.0 36.4 38.0 100-104 37.0637 38.0 38.0 38.0 36.0 38.0 105-109 36.94175 38.0 38.0 38.0 35.6 38.0 110-114 36.67945 38.0 38.0 38.0 35.0 38.0 115-119 36.606 38.0 38.0 38.0 34.8 38.0 120-124 36.65545 38.0 38.0 38.0 35.0 38.0 125-129 36.506 38.0 38.0 38.0 34.4 38.0 130-134 36.4449 38.0 38.0 38.0 34.2 38.0 135-139 36.2443 38.0 38.0 38.0 33.6 38.0 140-144 36.058499999999995 38.0 38.0 38.0 33.2 38.0 145-149 35.834450000000004 38.0 37.8 38.0 32.4 38.0 150-151 32.1175 35.5 32.0 38.0 16.5 38.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 9 1.0 10 1.0 11 0.0 12 0.0 13 0.0 14 0.0 15 3.0 16 0.0 17 1.0 18 0.0 19 1.0 20 4.0 21 2.0 22 2.0 23 4.0 24 6.0 25 5.0 26 11.0 27 10.0 28 7.0 29 19.0 30 36.0 31 38.0 32 49.0 33 68.0 34 86.0 35 171.0 36 382.0 37 3093.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 35.841059602649004 13.165562913907285 11.019867549668874 39.973509933774835 2 25.10627656914228 16.60415103775944 32.70817704426106 25.581395348837212 3 21.725 23.825 24.85 29.599999999999998 4 24.25 32.125 22.025 21.6 5 21.725 35.175 25.35 17.75 6 19.225 37.925 22.95 19.900000000000002 7 13.525 28.599999999999998 40.400000000000006 17.474999999999998 8 18.5 26.025 31.15 24.325 9 16.475 24.525 33.675 25.324999999999996 10-14 20.165 33.005 23.580000000000002 23.25 15-19 19.82 29.945 27.439999999999998 22.795 20-24 18.69 31.335 26.775 23.200000000000003 25-29 21.985 29.975 25.685000000000002 22.355 30-34 21.654999999999998 32.365 23.880000000000003 22.1 35-39 20.615 31.2 26.205000000000002 21.98 40-44 19.875 29.92 26.395000000000003 23.810000000000002 45-49 20.225 30.29 27.200000000000003 22.285 50-54 21.175 29.78 25.674999999999997 23.369999999999997 55-59 21.055 30.29 24.625 24.03 60-64 19.54 30.65 26.43 23.380000000000003 65-69 20.919999999999998 30.240000000000002 24.705 24.135 70-74 21.605 29.475 24.375 24.545 75-79 21.555 30.86 24.935 22.650000000000002 80-84 22.805 29.060000000000002 25.124999999999996 23.01 85-89 21.86 28.799999999999997 26.525 22.814999999999998 90-94 20.735 30.825000000000003 25.119999999999997 23.32 95-99 20.810000000000002 30.495 24.38 24.315 100-104 21.05526381595399 30.46761690422606 24.34108527131783 24.136034008502126 105-109 21.345 29.475 25.5 23.68 110-114 20.55976325425089 29.929277223253248 25.83638461152631 23.674574910969554 115-119 19.93084093414854 30.74070361832214 24.721860278640875 24.606595168888443 120-124 20.285 30.69 22.965 26.06 125-129 21.84 30.915 23.745 23.5 130-134 22.12 30.915 23.1 23.865 135-139 22.720000000000002 30.080000000000002 24.085 23.115 140-144 22.935 30.349999999999998 24.275 22.439999999999998 145-149 21.515 29.520000000000003 23.89 25.074999999999996 150-151 20.525 30.7 23.4375 25.337500000000002 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.5 5 0.5 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.5 12 1.5 13 1.0 14 0.0 15 0.0 16 0.5 17 0.5 18 0.5 19 0.5 20 1.5 21 2.0 22 1.0 23 2.0 24 4.0 25 6.0 26 6.5 27 8.0 28 9.5 29 16.5 30 25.5 31 28.5 32 37.5 33 42.5 34 48.0 35 60.5 36 118.0 37 240.5 38 244.0 39 197.0 40 251.0 41 265.5 42 224.5 43 223.5 44 226.0 45 216.0 46 171.0 47 122.0 48 123.5 49 98.5 50 88.0 51 77.0 52 60.5 53 70.0 54 72.0 55 82.5 56 87.5 57 71.5 58 64.5 59 64.0 60 50.0 61 33.5 62 23.0 63 19.5 64 24.0 65 26.0 66 16.0 67 7.5 68 5.5 69 4.5 70 6.5 71 7.5 72 5.0 73 2.5 74 1.5 75 1.5 76 1.5 77 0.5 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content warn #Base N-Count 1 5.625 2 0.025 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.025 105-109 0.0 110-114 0.315 115-119 0.22999999999999998 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 68.825 #Duplication Level Percentage of deduplicated Percentage of total 1 85.8699600435888 59.099999999999994 2 7.373774064656738 10.15 3 1.634580457682528 3.375 4 1.3439883763167455 3.6999999999999997 5 1.0170722847802398 3.5000000000000004 6 0.7628042135851798 3.15 7 0.32691609153650564 1.575 8 0.5811841627315656 3.2 9 0.25426807119505995 1.575 >10 0.7991282237559026 9.025 >50 0.03632401017072285 1.6500000000000001 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCATTAAT 66 1.6500000000000001 No Hit GGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTACGTT 29 0.7250000000000001 No Hit TTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCATTAATA 23 0.575 No Hit GCGGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTACG 23 0.575 No Hit CTCATAAGGACCACCATTGTATAACCATTCATCAACGGATGCAGCTTCCC 22 0.5499999999999999 No Hit GTTGAAACTAGCATATTGGAAGATTAATCGGCCAAAATAACCATGAGCAG 22 0.5499999999999999 No Hit GCCAAAATAACCATGAGCAGCCACAATATTATAAGTCTCTTCCTCTTGAC 19 0.475 No Hit GCGGAAAAACCTTATCCATTAATAGCGGGAACTTCAAGAGCAGCTAGATC 19 0.475 No Hit GCTTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCA 18 0.44999999999999996 No Hit CTTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCAT 18 0.44999999999999996 No Hit CGCAGAAGTAGGAATAATAGCACCAGAGATAATATTGTTTCCATAAAGTA 17 0.42500000000000004 No Hit GTGCTCTGCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTCCTA 16 0.4 No Hit TTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCATT 15 0.375 No Hit CATATAACAAGCTACACCAAGTAAGAAGTGTAGAACAATTAGCTCATAAG 15 0.375 No Hit TTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCATTA 13 0.325 No Hit CTCTTGACCAAATTTGTAACCCTCATTAGCAGATTCATTTTCAGTAGTTT 13 0.325 No Hit CTCGCGAATACCATCAATATCTACTGGAGGGGCTGCGATGAAGGCGATAA 13 0.325 No Hit CTGCGATGAAGGCGATAATAAATACAGAAGTTGCGGTCAATAAGGTAGGG 12 0.3 No Hit GCTAGTTATCCAGTTGCAGAAGCGACCCCACAGGCTTGTACTTTCGCGTC 12 0.3 No Hit GCCGAAAACACCAGCTACACCTAACATGTGAAATGGATGCATAAGGATGT 11 0.27499999999999997 No Hit CTCATTAGCAGATTCATTTTCAGTAGTTTCCCTGATCAAACTAGAGGTTA 11 0.27499999999999997 No Hit CTTGACTATCAACTACAGATTGGTTGAAATTGAATCCATTTAGGTTGAAC 10 0.25 No Hit CCAGATTCCTACTACAGGCCAAGCAGCCAAGAAGAAGTGTAAAGAACGAG 10 0.25 No Hit CGGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTACGT 9 0.22499999999999998 No Hit CAATAAGGTAGGGATCATCAAAACACCGAACCATCCGATGTAAAGACGAT 9 0.22499999999999998 No Hit GGCGCATACCCAAACGGAAACTAAGTTCCCACTCACGACCCATATAACAA 9 0.22499999999999998 No Hit CGGAAAAACCTTATCCATTAATAGCGGGAACTTCAAGAGCAGCTAGATCT 9 0.22499999999999998 No Hit GTTCGCTGGAGTAGGCTGCGAGGCCCAAGCGGCCAAGGAGTGCAGCGGCC 9 0.22499999999999998 No Hit GGCCAAGCAGCCAAGAAGAAGTGTAAAGAACGAGAGTTGTTGAAACTAGC 9 0.22499999999999998 No Hit GGTAAATCAAGAAAACAGCAGTCGCAGCTGCAACAGGAGCTGAATATGCA 9 0.22499999999999998 No Hit GTGCAATCCGATTGCCGCAGAAGTAGGAATAATAGCACCAGAGATAATAT 8 0.2 No Hit CCGCAGAAGTAGGAATAATAGCACCAGAGATAATATTGTTTCCATAAAGT 8 0.2 No Hit GGCCAAAATAACCATGAGCAGCCACAATATTATAAGTCTCTTCCTCTTGA 8 0.2 No Hit GTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTC 8 0.2 No Hit GGAGGGGCTGCGATGAAGGCGATAATAAATACAGAAGTTGCGGTCAATAA 8 0.2 No Hit CCACCATTGTATAACCATTCATCAACGGATGCAGCTTCCCAAATTGGGTA 8 0.2 No Hit CCCAAGTATTAATAACGCGACCTTGACTATCAACTACAGATTGGTTGAAA 8 0.2 No Hit GGATGCATAAGGATGTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAA 8 0.2 No Hit CCACAATATTATAAGTCTCTTCCTCTTGACCAAATTTGTAACCCTCATTA 8 0.2 No Hit CAGAGATAATATTGTTTCCATAAAGTAAAGAACCAGAAACAGGCTCGCGA 8 0.2 No Hit CAGATTCCTACTACAGGCCAAGCAGCCAAGAAGAAGTGTAAAGAACGAGA 8 0.2 No Hit CTAGCATATTGGAAGATTAATCGGCCAAAATAACCATGAGCAGCCACAAT 8 0.2 No Hit AGCGGAAAAACCTTATCCATTAATAGCGGGAACTTCAAGAGCAGCTAGAT 8 0.2 No Hit CACCATTGTATAACCATTCATCAACGGATGCAGCTTCCCAAATTGGGTAA 8 0.2 No Hit NTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCATTAAT 8 0.2 No Hit CCAGATATTCCTAAAGGCATACCATCAGAGAAGCTTCCTTGACCAATAGG 8 0.2 No Hit GTCGCAGCTGCAACAGGAGCTGAATATGCAACAGCAATCCAAGGGCGCAT 7 0.17500000000000002 No Hit TTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCATTAATAG 7 0.17500000000000002 No Hit TGATGATATCAGCCCAAGTATTAATAACGCGACCTTGACTATCAACTACA 7 0.17500000000000002 No Hit GCACTGAATAGGGAACCGCCGAAAACACCAGCTACACCTAACATGTGAAA 7 0.17500000000000002 No Hit GTGAGCATTACGTTCGTGCATTACTTCCATACCAAGATTAGCACGGTTGA 7 0.17500000000000002 No Hit GGGATCATCAAAACACCGAACCATCCGATGTAAAGACGATTTTCAGTGCT 7 0.17500000000000002 No Hit CATCAGAGAAGCTTCCTTGACCAATAGGGTAAATCAAGAAAACAGCAGTC 7 0.17500000000000002 No Hit GAAAAACCTTATCCATTAATAGCGGGAACTTCAAGAGCAGCTAGATCTAG 7 0.17500000000000002 No Hit GCATAAGGATGTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAAAGTA 7 0.17500000000000002 No Hit CCCGAAGTTACGGGGCTATTTTGCCGAGTTCCTTAGAGAGAGTTGTCTCG 6 0.15 No Hit GCACGGTTGATGATATCAGCCCAAGTATTAATAACGCGACCTTGACTATC 6 0.15 No Hit TGGAAGATTAATCGGCCAAAATAACCATGAGCAGCCACAATATTATAAGT 6 0.15 No Hit CGCCATAGTACTAATACCTAAAGCAGTGAACCAGATTCCTACTACAGGCC 6 0.15 No Hit AAGGATGTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAAAGTACCAG 6 0.15 No Hit TAAGGATGTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAAAGTACCA 6 0.15 No Hit AGATAATATTGTTTCCATAAAGTAAAGAACCAGAAACAGGCTCGCGAATA 6 0.15 No Hit ACCAGATATTCCTAAAGGCATACCATCAGAGAAGCTTCCTTGACCAATAG 6 0.15 No Hit CAAGTATTAATAACGCGACCTTGACTATCAACTACAGATTGGTTGAAATT 6 0.15 No Hit ATCCGATTGCCGCAGAAGTAGGAATAATAGCACCAGAGATAATATTGTTT 6 0.15 No Hit GGGAAGTTGTGAGCATTACGTTCGTGCATTACTTCCATACCAAGATTAGC 6 0.15 No Hit ATAAGGATGTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAAAGTACC 6 0.15 No Hit GCGACCTTGACTATCAACTACAGATTGGTTGAAATTGAATCCATTTAGGT 6 0.15 No Hit TGAAATTGAATCCATTTAGGTTGAACGCCATAGTACTAATACCTAAAGCA 6 0.15 No Hit TGCATAAGGATGTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAAAGT 6 0.15 No Hit GATGCATAAGGATGTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAAA 6 0.15 No Hit CATAAGGATGTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAAAGTAC 6 0.15 No Hit CTTTGGAGTAGGCTATGAGACCCAAGCGGGCCAGGAATGCAGCGGCCCGT 6 0.15 No Hit CGCGACCTTGACTATCAACTACAGATTGGTTGAAATTGAATCCATTTAGG 6 0.15 No Hit GCTCTGCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTCCTAAA 6 0.15 No Hit GGGGCTGCGATGAAGGCGATAATAAATACAGAAGTTGCGGTCAATAAGGT 6 0.15 No Hit GAGAAGCTTCCTTGACCAATAGGGTAAATCAAGAAAACAGCAGTCGCAGC 5 0.125 No Hit GGGCGCATACCCAAACGGAAACTAAGTTCCCACTCACGACCCATATAACA 5 0.125 No Hit ACCAGCTACACCTAACATGTGAAATGGATGCATAAGGATGTTGTGCTCTG 5 0.125 No Hit AAAAATTCTTATATGTTAGCGGAAAAACCTTATCCATTAATAGCGGGAAC 5 0.125 No Hit GTATTAATAACGCGACCTTGACTATCAACTACAGATTGGTTGAAATTGAA 5 0.125 No Hit GGAAGATTAATCGGCCAAAATAACCATGAGCAGCCACAATATTATAAGTC 5 0.125 No Hit GGAAAAACCTTATCCATTAATAGCGGGAACTTCAAGAGCAGCTAGATCTA 5 0.125 No Hit GGCGATAATAAATACAGAAGTTGCGGTCAATAAGGTAGGGATCATCAAAA 5 0.125 No Hit ATAAGGACCACCATTGTATAACCATTCATCAACGGATGCAGCTTCCCAAA 5 0.125 No Hit CCATATAACAAGCTACACCAAGTAAGAAGTGTAGAACAATTAGCTCATAA 5 0.125 No Hit GGGTAAATCAAGAAAACAGCAGTCGCAGCTGCAACAGGAGCTGAATATGC 5 0.125 No Hit GATGATATCAGCCCAAGTATTAATAACGCGACCTTGACTATCAACTACAG 5 0.125 No Hit CAGCAATCCAAGGGCGCATACCCAAACGGAAACTAAGTTCCCACTCACGA 5 0.125 No Hit CCCTCATTAGCAGATTCATTTTCAGTAGTTTCCCTGATCAAACTAGAGGT 5 0.125 No Hit CATGCTCCACCGCTTGTGCGGGCCCCCGTCAATTCCTTTGAGTTTCATTC 5 0.125 No Hit GCTGCGATGAAGGCGATAATAAATACAGAAGTTGCGGTCAATAAGGTAGG 5 0.125 No Hit ATGATATCAGCCCAAGTATTAATAACGCGACCTTGACTATCAACTACAGA 5 0.125 No Hit CCAAGTATTAATAACGCGACCTTGACTATCAACTACAGATTGGTTGAAAT 5 0.125 No Hit GTACAAGGCCCGGGAACGGATTCACCGCCGTATGGCTGACCGGCGATTAC 5 0.125 No Hit CCAAGATTAGCACGGTTGATGATATCAGCCCAAGTATTAATAACGCGACC 5 0.125 No Hit ATATAACAAGCTACACCAAGTAAGAAGTGTAGAACAATTAGCTCATAAGG 5 0.125 No Hit GAGCTGAATATGCAACAGCAATCCAAGGGCGCATACCCAAACGGAAACTA 5 0.125 No Hit TAAAGAACGAGAGTTGTTGAAACTAGCATATTGGAAGATTAATCGGCCAA 5 0.125 No Hit GGCAATTTCAAGCACTCTTTGACTCTCTTTTCAAAGTCCTTTTCATCTTT 5 0.125 No Hit GTGAAATGGATGCATAAGGATGTTGTGCTCTGCCTGGAATACAATCATAA 5 0.125 No Hit CAAATTTGTAACCCTCATTAGCAGATTCATTTTCAGTAGTTTCCCTGATC 5 0.125 No Hit GGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTACGTTC 5 0.125 No Hit CATAAGGACCACCATTGTATAACCATTCATCAACGGATGCAGCTTCCCAA 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.0 0.0 0.0 0.0 0.0 70-71 0.0 0.0 0.0 0.0 0.0 72-73 0.0 0.0 0.0 0.0 0.0 74-75 0.0 0.0 0.0 0.0 0.0 76-77 0.05 0.0 0.0 0.0 0.0 78-79 0.0875 0.0 0.0 0.0 0.0 80-81 0.1375 0.0 0.0 0.0 0.0 82-83 0.1875 0.0 0.0 0.0 0.0 84-85 0.25 0.0 0.0 0.0 0.0 86-87 0.35 0.0 0.0 0.0 0.0 88-89 0.475 0.0 0.0 0.0 0.0 90-91 0.6125 0.0 0.0 0.0 0.0 92-93 0.8125 0.0 0.0 0.0 0.0 94-95 0.9375 0.0 0.0 0.0 0.0 96-97 1.175 0.0 0.0 0.0 0.0 98-99 1.425 0.0 0.0 0.0 0.0 100-101 1.6625 0.0 0.0 0.0 0.0 102-103 2.075 0.0 0.0 0.0 0.0 104-105 2.4000000000000004 0.0 0.0 0.0 0.0 106-107 2.7125 0.0 0.0 0.0 0.0 108-109 3.0625 0.0 0.0 0.0 0.0 110-111 3.5 0.0 0.0 0.0 0.0 112-113 3.8625 0.0 0.0 0.0 0.0 114-115 4.225 0.0 0.0 0.0 0.0 116-117 4.65 0.0 0.0 0.0 0.0 118-119 5.125 0.0 0.0 0.0 0.0 120-121 5.7125 0.0 0.0 0.0 0.0 122-123 6.225 0.0 0.0 0.0 0.0 124-125 6.85 0.0 0.0 0.0 0.0 126-127 7.2625 0.0 0.0 0.0 0.0 128-129 7.7 0.0 0.0 0.0 0.0 130-131 8.4875 0.0 0.0 0.0 0.0 132-133 9.1125 0.0 0.0 0.0 0.0 134-135 9.925 0.0 0.0 0.0 0.0 136-137 10.75 0.0 0.0 0.0 0.0 138-139 11.2125 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCATACC 45 0.008978676 48.30417 6 CAGATCG 35 0.003549008 20.701786 130-134 TCGGAAG 60 2.2820105E-4 16.906458 135-139 AGAGCAC 60 2.2820105E-4 16.906458 140-144 ATCGGAA 60 2.2820105E-4 16.906458 135-139 GAGCACA 60 0.004509948 14.491249 140-144 CGGAAGA 60 0.004509948 14.491249 135-139 AAGAGCA 95 0.0073108436 10.677763 140-144 >>END_MODULE SRR6941597 read2 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR6941597_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 33.056 34.0 33.0 34.0 33.0 34.0 2 33.2455 34.0 33.0 34.0 33.0 34.0 3 33.25925 34.0 33.0 34.0 33.0 34.0 4 33.1825 34.0 33.0 34.0 33.0 34.0 5 33.2255 34.0 33.0 34.0 33.0 34.0 6 37.35425 38.0 38.0 38.0 38.0 38.0 7 37.371 38.0 38.0 38.0 38.0 38.0 8 37.3775 38.0 38.0 38.0 38.0 38.0 9 37.37725 38.0 38.0 38.0 38.0 38.0 10-14 37.282500000000006 38.0 38.0 38.0 37.6 38.0 15-19 37.297 38.0 38.0 38.0 37.8 38.0 20-24 37.34205 38.0 38.0 38.0 38.0 38.0 25-29 37.2555 38.0 38.0 38.0 37.0 38.0 30-34 37.2611 38.0 38.0 38.0 37.4 38.0 35-39 37.2892 38.0 38.0 38.0 37.6 38.0 40-44 37.26195 38.0 38.0 38.0 37.2 38.0 45-49 37.24005000000001 38.0 38.0 38.0 37.0 38.0 50-54 37.28285 38.0 38.0 38.0 37.4 38.0 55-59 37.18835 38.0 38.0 38.0 37.0 38.0 60-64 37.072649999999996 38.0 38.0 38.0 37.0 38.0 65-69 37.09205 38.0 38.0 38.0 36.8 38.0 70-74 37.05485 38.0 38.0 38.0 36.4 38.0 75-79 37.0438 38.0 38.0 38.0 36.8 38.0 80-84 37.0115 38.0 38.0 38.0 36.4 38.0 85-89 36.928250000000006 38.0 38.0 38.0 36.0 38.0 90-94 36.8644 38.0 38.0 38.0 36.0 38.0 95-99 36.80815 38.0 38.0 38.0 35.6 38.0 100-104 36.7162 38.0 38.0 38.0 35.0 38.0 105-109 36.46865 38.0 38.0 38.0 34.6 38.0 110-114 36.00555000000001 38.0 38.0 38.0 33.4 38.0 115-119 36.0079 38.0 37.8 38.0 33.2 38.0 120-124 35.9327 38.0 37.4 38.0 32.8 38.0 125-129 35.917649999999995 38.0 37.6 38.0 33.2 38.0 130-134 35.77865 38.0 37.6 38.0 32.4 38.0 135-139 35.357150000000004 38.0 36.0 38.0 31.0 38.0 140-144 34.9394 38.0 36.0 38.0 30.4 38.0 145-149 33.9861 38.0 35.6 38.0 24.6 38.0 150-151 28.710625 34.5 18.0 38.0 2.0 38.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 8.0 3 2.0 4 0.0 5 0.0 6 0.0 7 1.0 8 1.0 9 0.0 10 1.0 11 1.0 12 2.0 13 3.0 14 2.0 15 2.0 16 1.0 17 1.0 18 4.0 19 4.0 20 6.0 21 2.0 22 3.0 23 8.0 24 6.0 25 10.0 26 21.0 27 17.0 28 17.0 29 34.0 30 34.0 31 49.0 32 57.0 33 87.0 34 121.0 35 203.0 36 465.0 37 2827.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 36.875 17.299999999999997 17.849999999999998 27.975 2 27.275 18.0 36.05 18.675 3 19.075 21.9 39.550000000000004 19.475 4 23.599999999999998 29.325000000000003 28.175 18.9 5 26.35 31.0 25.15 17.5 6 19.85 32.65 29.375 18.125 7 17.4 19.3 43.824999999999996 19.475 8 22.225 21.775 31.025000000000002 24.975 9 22.2 20.599999999999998 33.5 23.7 10-14 24.310000000000002 25.585 29.4 20.705000000000002 15-19 24.12 24.65 30.935000000000002 20.294999999999998 20-24 24.505 25.03 30.769999999999996 19.695 25-29 24.525 24.959999999999997 29.475 21.04 30-34 24.435000000000002 24.525 30.415 20.625 35-39 24.055 25.39 29.465000000000003 21.09 40-44 23.735 25.814999999999998 30.03 20.419999999999998 45-49 23.5 26.715 28.83 20.955 50-54 23.35 25.8 29.78 21.07 55-59 23.225 25.314999999999998 29.805 21.654999999999998 60-64 24.07 24.63 30.69 20.61 65-69 24.72 25.465 28.95 20.865000000000002 70-74 24.335 24.98 30.349999999999998 20.335 75-79 24.535 25.130000000000003 29.4 20.935000000000002 80-84 24.395 24.044999999999998 31.275 20.285 85-89 23.87 26.169999999999998 28.544999999999998 21.415 90-94 23.97 25.105 29.315 21.61 95-99 23.275000000000002 24.62 30.725 21.38 100-104 24.815 24.85 30.36 19.975 105-109 25.240000000000002 24.27 29.604999999999997 20.885 110-114 24.15 24.95 30.43 20.47 115-119 24.605 25.174999999999997 29.675 20.544999999999998 120-124 24.169999999999998 26.695 28.535 20.599999999999998 125-129 24.355 25.665 28.860000000000003 21.12 130-134 24.83 26.58 28.384999999999998 20.205000000000002 135-139 25.019999999999996 26.169999999999998 29.285 19.525000000000002 140-144 26.0 26.435 28.08 19.485 145-149 25.06 24.875 29.544999999999998 20.52 150-151 25.26565820727591 24.515564445555693 30.45380672584073 19.764970621327667 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1.0 1 0.5 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.5 8 0.5 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.5 19 0.5 20 1.5 21 2.0 22 1.0 23 1.5 24 4.5 25 9.0 26 11.0 27 12.5 28 16.0 29 22.5 30 24.5 31 23.0 32 30.0 33 40.0 34 60.5 35 79.5 36 101.5 37 143.5 38 182.5 39 204.0 40 232.0 41 244.0 42 231.5 43 244.5 44 256.0 45 221.0 46 186.0 47 149.0 48 109.0 49 88.5 50 80.0 51 85.0 52 75.5 53 66.0 54 70.5 55 85.0 56 79.5 57 58.5 58 64.5 59 74.0 60 62.0 61 51.0 62 49.5 63 37.5 64 22.0 65 18.0 66 11.0 67 9.0 68 12.0 69 10.0 70 9.0 71 8.5 72 7.5 73 7.0 74 4.5 75 2.0 76 2.0 77 2.0 78 1.5 79 0.5 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0125 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 68.72500000000001 #Duplication Level Percentage of deduplicated Percentage of total 1 83.88504910876684 57.65 2 7.602764641687887 10.45 3 3.2375409239723534 6.675000000000001 4 1.4550745725718441 4.0 5 1.054929065114587 3.6249999999999996 6 0.6184066933430339 2.55 7 0.4728992360858494 2.275 8 0.43652237177155323 2.4 9 0.327391778828665 2.025 >10 0.9094216078574027 8.35 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source ATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCA 28 0.7000000000000001 No Hit ATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAA 26 0.65 No Hit GCTTGTTATATGGGTCGTGAGTGGGAACTTAGTTTCCGTTTGGGTATGCG 24 0.6 No Hit TATCTATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTT 18 0.44999999999999996 No Hit GCTAGTTTCAACAACTCTCGTTCTTTACACTTCTTCTTGGCTGCTTGGCC 17 0.42500000000000004 No Hit GGTCGCTTCTGCAACTGGATAACTAGCACTGAAAATCGTCTTTACATCGG 16 0.4 No Hit CTATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAA 14 0.35000000000000003 No Hit GTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGATCTTACCATGAC 14 0.35000000000000003 No Hit ATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCTAAATGG 13 0.325 No Hit GTTCGATCCTGGCTCAGGATGAACGCTGGCGGCATGCTTAACACATGCAA 12 0.3 No Hit ATCGGATTGCACTTTTACCCAATTTGGGAAGCTGCATCCGTTGATGAATG 11 0.27499999999999997 No Hit GGGAACTTAGTTTCCGTTTGGGTATGCGCCCTTGGATTGCTGTTGCATAT 11 0.27499999999999997 No Hit AGCACAACATCCTTATGCATCCATTTCACATGTTAGGTGTAGCTGGTGTT 10 0.25 No Hit TTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAA 10 0.25 No Hit CCTAAATGGATTCAATTTCAACCAATCTGTAGTTGATAGTCAAGGTCGCG 10 0.25 No Hit TGGGAACTTAGTTTCCGTTTGGGTATGCGCCCTTGGATTGCTGTTGCATA 10 0.25 No Hit CTTTACTTTATGGAAACAATATTATCTCTGGTGCTATTATTCCTACTTCT 10 0.25 No Hit TTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAG 10 0.25 No Hit GTTGCATATTCAGCTCCTGTTGCAGCTGCGACTGCTGTTTTCTTGATTTA 10 0.25 No Hit CGCGAAAGTACAAGCCTGTGGGGTCGCTTCTGCAACTGGATAACTAGCAC 10 0.25 No Hit GCTGCGACTGCTGTTTTCTTGATTTACCCTATTGGTCAAGGAAGCTTCTC 10 0.25 No Hit GCACAACATCCTTATGCATCCATTTCACATGTTAGGTGTAGCTGGTGTTT 10 0.25 No Hit GTTCAACCTAAATGGATTCAATTTCAACCAATCTGTAGTTGATAGTCAAG 10 0.25 No Hit GTTTGATCAGGGAAACTACTGAAAATGAATCTGCTAATGAGGGTTACAAA 10 0.25 No Hit GGTACTTTCAACTTTATGATTGTATTCCAGGCAGAGCACAACATCCTTAT 10 0.25 No Hit GGAAACTACTGAAAATGAATCTGCTAATGAGGGTTACAAATTTGGTCAAG 9 0.22499999999999998 No Hit TGAAAATGAATCTGCTAATGAGGGTTACAAATTTGGTCAAGAGGAAGAGA 9 0.22499999999999998 No Hit CTGGATAACTAGCACTGAAAATCGTCTTTACATCGGATGGTTCGGTGTTT 9 0.22499999999999998 No Hit ATTATCTCTGGTGCTATTATTCCTACTTCTGCGGCAATCGGATTGCACTT 9 0.22499999999999998 No Hit GGAAGCTTCTCTGATGGTATGCCTTTAGGAATATCTGGTACTTTCAACTT 9 0.22499999999999998 No Hit GTGGGAACTTAGTTTCCGTTTGGGTATGCGCCCTTGGATTGCTGTTGCAT 9 0.22499999999999998 No Hit CCTTACCAGGGCTTGACATGCCGCGAATCCTCTTGAAAGAGAGGGGTGCC 9 0.22499999999999998 No Hit CTTCCAATATGCTAGTTTCAACAACTCTCGTTCTTTACACTTCTTCTTGG 9 0.22499999999999998 No Hit TGATAGTCAAGGTCGCGTTATTAATACTTGGGCTGATATCATCAACCGTG 9 0.22499999999999998 No Hit CCGCAACTTCTGTATTTATTATCGCCTTCATCGCAGCCCCTCCAGTAGAT 8 0.2 No Hit CTCGTTCTTTACACTTCTTCTTGGCTGCTTGGCCTGTAGTAGGAATCTGG 8 0.2 No Hit ATTTGGTCAAGAGGAAGAGACTTATAATATTGTGGCTGCTCATGGTTATT 8 0.2 No Hit CATGACTGCAATTTTAGAGAGACGCGAAAGTACAAGCCTGTGGGGTCGCT 8 0.2 No Hit GTTATTTTGGCCGATTAATCTTCCAATATGCTAGTTTCAACAACTCTCGT 8 0.2 No Hit GTTTTGATGATCCCTACCTTATTGACCGCAACTTCTGTATTTATTATCGC 8 0.2 No Hit TCTAGTTTGATCAGGGAAACTACTGAAAATGAATCTGCTAATGAGGGTTA 8 0.2 No Hit AGTCAAGGTCGCGTTATTAATACTTGGGCTGATATCATCAACCGTGCTAA 8 0.2 No Hit TGTAGCTGGTGTTTTCGGCGGTTCCCTATTCAGTGCTATGCATGGTTCCT 8 0.2 No Hit GTCAAGGTCGCGTTATTAATACTTGGGCTGATATCATCAACCGTGCTAAT 8 0.2 No Hit ATCTATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTG 8 0.2 No Hit AGCTTGTTATATGGGTCGTGAGTGGGAACTTAGTTTCCGTTTGGGTATGC 8 0.2 No Hit CCTACTTCTGCGGCAATCGGATTGCACTTTTACCCAATTTGGGAAGCTGC 7 0.17500000000000002 No Hit CTTTTACCCAATTTGGGAAGCTGCATCCGTTGATGAATGGTTATACAATG 7 0.17500000000000002 No Hit TATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAA 7 0.17500000000000002 No Hit GGGAAACTACTGAAAATGAATCTGCTAATGAGGGTTACAAATTTGGTCAA 7 0.17500000000000002 No Hit CCAAGATCTTACCATGACTGCAATTTTAGAGAGACGCGAAAGTACAAGCC 7 0.17500000000000002 No Hit CGTCTTTACATCGGATGGTTCGGTGTTTTGATGATCCCTACCTTATTGAC 7 0.17500000000000002 No Hit CTGCTAATGAGGGTTACAAATTTGGTCAAGAGGAAGAGACTTATAATATT 7 0.17500000000000002 No Hit TATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACC 7 0.17500000000000002 No Hit CCTATTCAGTGCTATGCATGGTTCCTTGGTAACCTCTAGTTTGATCAGGG 7 0.17500000000000002 No Hit GTTTCTGGTTCTTTACTTTATGGAAACAATATTATCTCTGGTGCTATTAT 7 0.17500000000000002 No Hit CTTGGCTGCTTGGCCTGTAGTAGGAATCTGGTTCACTGCTTTAGGTATTA 7 0.17500000000000002 No Hit TCGGATTGCACTTTTACCCAATTTGGGAAGCTGCATCCGTTGATGAATGG 7 0.17500000000000002 No Hit GTTAGGTGTAGCTGGTGTTTTCGGCGGTTCCCTATTCAGTGCTATGCATG 7 0.17500000000000002 No Hit CTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGAT 6 0.15 No Hit CTGCTTTAGGTATTAGTACTATGGCGTTCAACCTAAATGGATTCAATTTC 6 0.15 No Hit GATGGTATTCGCGAGCCTGTTTCTGGTTCTTTACTTTATGGAAACAATAT 6 0.15 No Hit GTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGATCTT 6 0.15 No Hit GTACTATGGCGTTCAACCTAAATGGATTCAATTTCAACCAATCTGTAGTT 6 0.15 No Hit CGTTGATGAATGGTTATACAATGGTGGTCCTTATGAGCTAATTGTTCTAC 6 0.15 No Hit CTCTAGTTTGATCAGGGAAACTACTGAAAATGAATCTGCTAATGAGGGTT 6 0.15 No Hit CTTGTTATATGGGTCGTGAGTGGGAACTTAGTTTCCGTTTGGGTATGCGC 6 0.15 No Hit CTAGCACTGAAAATCGTCTTTACATCGGATGGTTCGGTGTTTTGATGATC 6 0.15 No Hit GGCTTGCGGTGGATACCTAGGCACCCAGAGACGAGGAAGGGCGTAGCAAG 6 0.15 No Hit CATCAACCGTGCTAATCTTGGTATGGAAGTAATGCACGAACGTAATGCTC 6 0.15 No Hit AGCTGGTGTTTTCGGCGGTTCCCTATTCAGTGCTATGCATGGTTCCTTGG 6 0.15 No Hit CAATGGTGGTCCTTATGAGCTAATTGTTCTACACTTCTTACTTGGTGTAG 6 0.15 No Hit TGATGGTATTCGCGAGCCTGTTTCTGGTTCTTTACTTTATGGAAACAATA 6 0.15 No Hit TGGCGTTCAACCTAAATGGATTCAATTTCAACCAATCTGTAGTTGATAGT 6 0.15 No Hit AGGGAAACTACTGAAAATGAATCTGCTAATGAGGGTTACAAATTTGGTCA 6 0.15 No Hit CAACATCCTTATGCATCCATTTCACATGTTAGGTGTAGCTGGTGTTTTCG 6 0.15 No Hit GGAAGAGACTTATAATATTGTGGCTGCTCATGGTTATTTTGGCCGATTAA 5 0.125 No Hit ATTCCTACTTCTGCGGCAATCGGATTGCACTTTTACCCAATTTGGGAAGC 5 0.125 No Hit GGTAACCTCTAGTTTGATCAGGGAAACTACTGAAAATGAATCTGCTAATG 5 0.125 No Hit CGGATTGCACTTTTACCCAATTTGGGAAGCTGCATCCGTTGATGAATGGT 5 0.125 No Hit CTGGTACTTTCAACTTTATGATTGTATTCCAGGCAGAGCACAACATCCTT 5 0.125 No Hit ATTTTGGCCGATTAATCTTCCAATATGCTAGTTTCAACAACTCTCGTTCT 5 0.125 No Hit TGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGATCTTACCATGACT 5 0.125 No Hit TATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAAT 5 0.125 No Hit CTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCTAAATGGAT 5 0.125 No Hit CCTGTAGTAGGAATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTT 5 0.125 No Hit GGAATATCTGGTACTTTCAACTTTATGATTGTATTCCAGGCAGAGCACAA 5 0.125 No Hit TGATGATCCCTACCTTATTGACCGCAACTTCTGTATTTATTATCGCCTTC 5 0.125 No Hit CGCAGCCCCTCCAGTAGATATTGATGGTATTCGCGAGCCTGTTTCTGGTT 5 0.125 No Hit AGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGATCT 5 0.125 No Hit CTTCTTGGCTGCTTGGCCTGTAGTAGGAATCTGGTTCACTGCTTTAGGTA 5 0.125 No Hit GCTGTTTTCTTGATTTACCCTATTGGTCAAGGAAGCTTCTCTGATGGTAT 5 0.125 No Hit GTTATACAATGGTGGTCCTTATGAGCTAATTGTTCTACACTTCTTACTTG 5 0.125 No Hit GTTGATAGTCAAGGTCGCGTTATTAATACTTGGGCTGATATCATCAACCG 5 0.125 No Hit CTTAGTTTCCGTTTGGGTATGCGCCCTTGGATTGCTGTTGCATATTCAGC 5 0.125 No Hit CTTACCATGACTGCAATTTTAGAGAGACGCGAAAGTACAAGCCTGTGGGG 5 0.125 No Hit TGCTAATGAGGGTTACAAATTTGGTCAAGAGGAAGAGACTTATAATATTG 5 0.125 No Hit GTAACCTCTAGTTTGATCAGGGAAACTACTGAAAATGAATCTGCTAATGA 5 0.125 No Hit CGAAAGTACAAGCCTGTGGGGTCGCTTCTGCAACTGGATAACTAGCACTG 5 0.125 No Hit TCCAGGCAGAGCACAACATCCTTATGCATCCATTTCACATGTTAGGTGTA 5 0.125 No Hit CGGCAATCGGATTGCACTTTTACCCAATTTGGGAAGCTGCATCCGTTGAT 5 0.125 No Hit TCTATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGA 5 0.125 No Hit CGCAACTTCTGTATTTATTATCGCCTTCATCGCAGCCCCTCCAGTAGATA 5 0.125 No Hit CTTCATCGCAGCCCCTCCAGTAGATATTGATGGTATTCGCGAGCCTGTTT 5 0.125 No Hit GATGATCCCTACCTTATTGACCGCAACTTCTGTATTTATTATCGCCTTCA 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.0 0.0 0.0 0.0 0.0 70-71 0.0 0.0 0.0 0.0 0.0 72-73 0.0 0.0 0.0 0.0 0.0 74-75 0.0 0.0 0.0 0.0 0.0 76-77 0.05 0.0 0.0 0.0 0.0 78-79 0.0875 0.0 0.0 0.0 0.0 80-81 0.1375 0.0 0.0 0.0 0.0 82-83 0.1875 0.0 0.0 0.0 0.0 84-85 0.25 0.0 0.0 0.0 0.0 86-87 0.35 0.0 0.0 0.0 0.0 88-89 0.475 0.0 0.0 0.0 0.0 90-91 0.6125 0.0 0.0 0.0 0.0 92-93 0.8125 0.0 0.0 0.0 0.0 94-95 0.9375 0.0 0.0 0.0 0.0 96-97 1.175 0.0 0.0 0.0 0.0 98-99 1.425 0.0 0.0 0.0 0.0 100-101 1.6625 0.0 0.0 0.0 0.0 102-103 2.0875 0.0 0.0 0.0 0.0 104-105 2.425 0.0 0.0 0.0 0.0 106-107 2.7375 0.0 0.0 0.0 0.0 108-109 3.0875000000000004 0.0 0.0 0.0 0.0 110-111 3.5 0.0 0.0 0.0 0.0 112-113 3.8625 0.0 0.0 0.0 0.0 114-115 4.225 0.0 0.0 0.0 0.0 116-117 4.65 0.0 0.0 0.0 0.0 118-119 5.1125 0.0 0.0 0.0 0.0 120-121 5.725 0.0 0.0 0.0 0.0 122-123 6.25 0.0 0.0 0.0 0.0 124-125 6.9 0.0 0.0 0.0 0.0 126-127 7.325 0.0 0.0 0.0 0.0 128-129 7.775 0.0 0.0 0.0 0.0 130-131 8.5625 0.0 0.0 0.0 0.0 132-133 9.1875 0.0 0.0 0.0 0.0 134-135 10.025 0.0 0.0 0.0 0.0 136-137 10.85 0.0 0.0 0.0 0.0 138-139 11.2875 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AAGAGCG 60 2.2715089E-4 16.916668 140-144 TCGGAAG 60 2.2715089E-4 16.916668 135-139 AGAGCGT 65 4.1823133E-4 15.615384 140-144 ATCGGAA 65 4.1823133E-4 15.615384 135-139 GAGCGTC 60 0.004491891 14.500001 140-144 CGGAAGA 60 0.004491891 14.500001 135-139 >>END_MODULE Read 963058 spots for SRR6941597.sra Written 963058 spots for SRR6941597.sra Read 963058 spots for SRR6941597.sra Written 963058 spots for SRR6941597.sra Read 963058 spots for SRR6941597.sra Written 963058 spots for SRR6941597.sra Read 963058 spots for SRR6941597.sra Written 963058 spots for SRR6941597.sra Read 963058 spots for SRR6941597.sra Written 963058 spots for SRR6941597.sra Read 963058 spots for SRR6941597.sra Written 963058 spots for SRR6941597.sra Read 963058 spots for SRR6941597.sra Written 963058 spots for SRR6941597.sra Read 963058 spots for SRR6941597.sra Written 963058 spots for SRR6941597.sra Read 963058 spots for SRR6941597.sra Written 963058 spots for SRR6941597.sra Read 963058 spots for SRR6941597.sra Written 963058 spots for SRR6941597.sra Read 963068 spots for SRR6941597.sra Written 963068 spots for SRR6941597.sra Read 963058 spots for SRR6941597.sra Written 963058 spots for SRR6941597.sra Read 963058 spots for SRR6941597.sra Read 963058 spots for SRR6941597.sra Written 963058 spots for SRR6941597.sra Written 963058 spots for SRR6941597.sra Read 963058 spots for SRR6941597.sra Written 963058 spots for SRR6941597.sra Read 963058 spots for SRR6941597.sra Written 963058 spots for SRR6941597.sra Read 963058 spots for SRR6941597.sra Written 963058 spots for SRR6941597.sra Read 963058 spots for SRR6941597.sra Written 963058 spots for SRR6941597.sra Read 963058 spots for SRR6941597.sra Written 963058 spots for SRR6941597.sra Read 963058 spots for SRR6941597.sra Written 963058 spots for SRR6941597.sra SRR ids: ['SRR6941597.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_o2j6ncsf SRR6941597.sra spots: 19261170 blocks: [[1, 963058], [963059, 1926116], [1926117, 2889174], [2889175, 3852232], [3852233, 4815290], [4815291, 5778348], [5778349, 6741406], [6741407, 7704464], [7704465, 8667522], [8667523, 9630580], [9630581, 10593638], [10593639, 11556696], [11556697, 12519754], [12519755, 13482812], [13482813, 14445870], [14445871, 15408928], [15408929, 16371986], [16371987, 17335044], [17335045, 18298102], [18298103, 19261170]] SRR6941597 file size 6505278 SRR6941597 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR6941597 SRR6941597_1.fastq SRR6941597_2.fastq Input file: SRR6941597_1.fastq Paired file: SRR6941597_2.fastq trimmed: SRR6941597-trimmed-pair1.fastq, SRR6941597-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Fri Dec 6 12:42:31 2024 >> started Fri Dec 6 12:42:51 2024 >> done (19.880s) 19261170 read pairs processed; of these: 9763 ( 0.05%) short read pairs filtered out after trimming by size control 9955 ( 0.05%) empty read pairs filtered out after trimming by size control 19241452 (99.90%) read pairs available; of these: 9714458 (50.49%) trimmed read pairs available after processing 9526994 (49.51%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 4 0.00% 19 1 0.00% 20 3 0.00% 21 3 0.00% 22 0 0.00% 23 2 0.00% 24 4 0.00% 25 1 0.00% 26 5 0.00% 27 13 0.00% 28 2 0.00% 29 7 0.00% 30 0 0.00% 31 7 0.00% 32 3 0.00% 33 7 0.00% 34 10 0.00% 35 4 0.00% 36 9 0.00% 37 4 0.00% 38 10 0.00% 39 12 0.00% 40 7 0.00% 41 20 0.00% 42 15 0.00% 43 28 0.00% 44 15 0.00% 45 24 0.00% 46 34 0.00% 47 31 0.00% 48 34 0.00% 49 60 0.00% 50 59 0.00% 51 84 0.00% 52 80 0.00% 53 102 0.00% 54 92 0.00% 55 121 0.00% 56 122 0.00% 57 179 0.00% 58 188 0.00% 59 248 0.00% 60 227 0.00% 61 328 0.00% 62 402 0.00% 63 480 0.00% 64 490 0.00% 65 582 0.00% 66 628 0.00% 67 778 0.00% 68 839 0.00% 69 949 0.00% 70 1038 0.01% 71 1283 0.01% 72 1623 0.01% 73 1712 0.01% 74 2006 0.01% 75 2417 0.01% 76 2604 0.01% 77 3001 0.02% 78 3240 0.02% 79 3765 0.02% 80 4311 0.02% 81 4909 0.03% 82 5374 0.03% 83 5960 0.03% 84 7108 0.04% 85 8389 0.04% 86 8810 0.05% 87 10086 0.05% 88 11451 0.06% 89 12010 0.06% 90 13430 0.07% 91 13691 0.07% 92 16562 0.09% 93 17103 0.09% 94 18125 0.09% 95 20767 0.11% 96 20269 0.11% 97 21644 0.11% 98 24172 0.13% 99 25095 0.13% 100 25914 0.13% 101 27483 0.14% 102 29600 0.15% 103 29799 0.15% 104 32704 0.17% 105 34479 0.18% 106 34695 0.18% 107 37580 0.20% 108 38759 0.20% 109 40384 0.21% 110 40296 0.21% 111 43254 0.22% 112 43801 0.23% 113 44554 0.23% 114 49184 0.26% 115 52977 0.28% 116 54530 0.28% 117 55897 0.29% 118 54809 0.28% 119 54619 0.28% 120 55968 0.29% 121 60578 0.31% 122 66051 0.34% 123 66159 0.34% 124 68704 0.36% 125 73770 0.38% 126 69244 0.36% 127 73194 0.38% 128 75187 0.39% 129 78071 0.41% 130 74112 0.39% 131 79021 0.41% 132 83898 0.44% 133 82206 0.43% 134 87859 0.46% 135 86864 0.45% 136 89919 0.47% 137 92022 0.48% 138 100423 0.52% 139 105609 0.55% 140 107321 0.56% 141 125978 0.65% 142 126465 0.66% 143 140695 0.73% 144 154974 0.81% 145 185934 0.97% 146 212646 1.11% 147 268883 1.40% 148 402470 2.09% 149 748410 3.89% 150 4547193 23.63% 151 9526994 49.51% 19241452 reads passed initial QC criterion=sequence-density sequence-density=0.70 sequence-density-rank=1 fanout-score=1.92 fanout-score-rank=32 prefix-density=0.69 prefix-fanout=1.9 sequence=GTATTTAGCCTTG criterion=fanout-score sequence-density=0.01 sequence-density-rank=41 fanout-score=57.18 fanout-score-rank=1 prefix-density=0.06 prefix-fanout=6.5 sequence=AAAAAAAAAGGGGGGTAAGGACCCGCTAAGCTCCTACTTTTTCATGTTTCCAATCCGATCCCTCCGATTACTATAGAGATGAACCCAATCCAGAATATGAACCATAAAAGAAAACACCTACTAAACCAATCACAAGAATACCAGTTACCGTACCTATCAGCCAAAGAGGAATTCTTCCAGTAGTATCGGCCATTTCCCCTACTTTCCTCCACATTTTATCAAGTGGTCATGCTAGAGACAAAAACAGTCATGGATAGTTATGTTATAAGGATGGTATCCTTCCAAATGGGATAAGAGAGTTCTTACTACTCTCTTCTTTTCTCTCAATTAAAGAAGTAATTGGAAAACAAAACAGCAAGTACAAAAATGAGTAATAAACCCCAGTATAGACTGGTACGATTCAATTCAACATTTTGTTCATTCGGGTTTGATTGTGTCATAGTTCTATAGTTGGAATTTAGTTTATCGTTGGATGAACTGCATTGCTGATATTGATCCCAAGAAAAAAACA criterion=sequence-density sequence-density=0.46 sequence-density-rank=1 fanout-score=2.89 fanout-score-rank=23 prefix-density=1.32 prefix-fanout=1.0 sequence=TTGCGTAGTGGATCTGCTGGGGCCTATGCGAAAGCTGGGCCTCACGGATCCTAGAGCGGCAGGCACCGCGTGAGGCTG criterion=fanout-score sequence-density=0.01 sequence-density-rank=33 fanout-score=20.71 fanout-score-rank=1 prefix-density=0.04 prefix-fanout=5.5 sequence=TTTTTTTTTATGAGATTTTTGCTAAAGTTTCATTTACGCCTAATTCACATCGAGTAGACCTTGTTATTGTGAGAATTCTTAATTCAAGAGTTGTAAGGAGGGACTTATGTCACCACAAACAGAAACTAAAGCAAGTGTTGGATTTAAAGCTGGTGTTAAAGATTATAGATTGACTTACTACACCCCGGAGTATGAAACCAAGGATACTGATATCTTGGCAGCATTCCGAGTATCTCCTCAACCTGGGGTTCCGCCCGAAGAAGCAGGGGCTGCAGTAGCTGCCGAATCTTCTACTGGTACATGGACAACTGTTTGGACTGATGGACTTACTAGTCTTGATCGTTACAAAGGACGATGCTATCACATCGAGCCTGTTCCTGGGGAAGACAGTCAATGGATCTGTTATGTAGCTTATCCATTAGATCTATTTGAAGAGGGTTCCGTTACTAACATGTTTACTTCCATTGTAGGTAACGTATTTGGTTTCAAAGCCCTACGTGCTCTACGTCTG SRR6941597 testing PE reads STAR mapping to Ensembl genome Started job on | Dec 06 12:43:33 Started mapping on | Dec 06 12:43:33 Finished on | Dec 06 12:45:28 Mapping speed, Million of reads per hour | 602.34 Number of input reads | 19241452 Average input read length | 291 UNIQUE READS: Uniquely mapped reads number | 11930878 Uniquely mapped reads % | 62.01% Average mapped length | 293.52 Number of splices: Total | 2338830 Number of splices: Annotated (sjdb) | 2117608 Number of splices: GT/AG | 2235678 Number of splices: GC/AG | 27947 Number of splices: AT/AC | 8211 Number of splices: Non-canonical | 66994 Mismatch rate per base, % | 0.31% Deletion rate per base | 0.02% Deletion average length | 1.98 Insertion rate per base | 0.02% Insertion average length | 2.38 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 6033922 % of reads mapped to multiple loci | 31.36% Number of reads mapped to too many loci | 87194 % of reads mapped to too many loci | 0.45% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 3.81% % of reads unmapped: other | 2.38% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 1283071 1283071 1283071 N_multimapping 6033922 6033922 6033922 N_noFeature 4878096 11537420 5043584 N_ambiguous 417356 4681 194974 UnstrandedReadsAssigned:6635426 PositiveStrandReadsAssigned:388777 NegativeStrandReadsAssigned:6692320 Dataset is classified negative stranded MeadianReadLen=151 20thPercentileLength=149 echo kmer=145 SRR6941597 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,972 [index] number of k-mers: 66,720,672 [index] number of equivalence classes: 111,837 [quant] running in paired-end mode [quant] will process pair 1: SRR6941597-trimmed-pair1.fastq SRR6941597-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 19,241,452 reads, 10,063,270 reads pseudoaligned [quant] estimated average fragment length: 222.092 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,028 rounds 52973 SRR6941597.ke.tsv 35125 SRR6941597.se.tsv 88098 total ==> SRR6941597.ke.tsv <== target_id length eff_length est_counts tpm PNS24245 936 715.279 0 0 PNS24247 1044 822.908 13.3658 1.49936 PNS24249 1928 1706.91 16.6056 0.898057 PNS24246 1044 822.908 13.3658 1.49936 PNS24248 1044 822.908 13.3658 1.49936 PNS24244 1471 1249.91 31.2969 2.31145 PNS24243 293 110.436 0 0 KQK14069 1603 1381.91 2261.04 151.039 KQK14071 474 263.698 58.9627 20.641 ==> SRR6941597.se.tsv <== BRADI_1g14170v3 2852 BRADI_1g53295v3 32 BRADI_1g59795v3 55 BRADI_1g07683v3 0 BRADI_1g00485v3 0 BRADI_1g20270v3 55 BRADI_1g74790v3 6 BRADI_1g09890v3 0 BRADI_1g77505v3 52 BRADI_1g48960v3 0 SRR6941597 completed mapping pipeline successfully