Starting /dee2/code/volunteer_pipeline.sh SRR6941598 current disk space = 1551375859712 free memory = 1599163196 SRR6941598 SRAfilesize a54dbf97f393412c398eddd726d61c09 SRR6941598.sra SRR6941598.sra file validated SRR6941598 is paired end SRR6941598 is conventional basespace SRR6941598 read1 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR6941598_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 19.221 18.0 18.0 18.0 18.0 32.0 2 25.73725 27.0 25.0 28.0 18.0 30.0 3 25.826 27.0 25.0 29.0 18.0 31.0 4 28.9185 30.0 27.0 31.0 25.0 33.0 5 30.931 32.0 32.0 33.0 27.0 33.0 6 35.916 37.0 36.0 38.0 33.0 38.0 7 37.06225 38.0 37.0 38.0 35.0 38.0 8 37.15875 38.0 38.0 38.0 36.0 38.0 9 37.3945 38.0 38.0 38.0 37.0 38.0 10-14 37.5098 38.0 38.0 38.0 37.2 38.0 15-19 37.415299999999995 38.0 38.0 38.0 37.2 38.0 20-24 37.545049999999996 38.0 38.0 38.0 37.8 38.0 25-29 37.6633 38.0 38.0 38.0 38.0 38.0 30-34 37.6673 38.0 38.0 38.0 38.0 38.0 35-39 37.6074 38.0 38.0 38.0 38.0 38.0 40-44 37.5886 38.0 38.0 38.0 38.0 38.0 45-49 37.552749999999996 38.0 38.0 38.0 37.8 38.0 50-54 37.40985 38.0 38.0 38.0 37.0 38.0 55-59 37.3042 38.0 38.0 38.0 37.0 38.0 60-64 37.43825 38.0 38.0 38.0 37.0 38.0 65-69 37.34865 38.0 38.0 38.0 36.8 38.0 70-74 37.40675 38.0 38.0 38.0 37.0 38.0 75-79 37.36905 38.0 38.0 38.0 37.0 38.0 80-84 37.38075 38.0 38.0 38.0 37.0 38.0 85-89 37.249199999999995 38.0 38.0 38.0 36.6 38.0 90-94 36.2501 38.0 37.2 38.0 30.6 38.0 95-99 36.82665 38.0 37.8 38.0 35.4 38.0 100-104 37.152249999999995 38.0 38.0 38.0 36.2 38.0 105-109 37.02795 38.0 38.0 38.0 35.8 38.0 110-114 37.024950000000004 38.0 38.0 38.0 35.8 38.0 115-119 36.8326 38.0 38.0 38.0 34.8 38.0 120-124 36.79015 38.0 38.0 38.0 35.0 38.0 125-129 36.7194 38.0 38.0 38.0 34.8 38.0 130-134 36.7153 38.0 38.0 38.0 35.0 38.0 135-139 36.3571 38.0 38.0 38.0 33.8 38.0 140-144 36.1612 38.0 38.0 38.0 33.4 38.0 145-149 35.745050000000006 38.0 37.4 38.0 33.0 38.0 150-151 33.157125 37.0 34.0 38.0 16.5 38.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 12 1.0 13 0.0 14 2.0 15 0.0 16 0.0 17 0.0 18 1.0 19 0.0 20 1.0 21 0.0 22 1.0 23 3.0 24 7.0 25 7.0 26 6.0 27 12.0 28 10.0 29 13.0 30 21.0 31 42.0 32 55.0 33 84.0 34 106.0 35 231.0 36 631.0 37 2766.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 29.443544030253914 20.961642355483523 10.237709346299297 39.35710426796326 2 25.431357839459867 18.42960740185046 31.807951987997 24.33108277069267 3 20.025000000000002 24.4 25.75 29.825000000000003 4 22.5 33.725 21.725 22.05 5 22.125 36.449999999999996 23.025000000000002 18.4 6 18.0 37.625 25.55 18.825 7 12.475 28.675 42.225 16.625 8 18.375 26.6 30.925000000000004 24.099999999999998 9 16.85 23.0 34.2 25.95 10-14 19.925 32.574999999999996 23.995 23.505000000000003 15-19 19.975 30.185000000000002 26.27 23.57 20-24 19.634999999999998 30.995 26.119999999999997 23.25 25-29 21.805 30.45 25.88 21.865000000000002 30-34 21.265 31.669999999999998 24.955 22.11 35-39 20.785 30.085 26.36 22.770000000000003 40-44 19.470000000000002 30.09 26.68 23.76 45-49 20.0 30.044999999999998 27.48 22.475 50-54 21.01 30.3 26.224999999999998 22.465 55-59 21.099999999999998 30.064999999999998 25.88 22.955000000000002 60-64 19.445 30.4 26.755000000000003 23.400000000000002 65-69 21.17 30.509999999999998 24.95 23.369999999999997 70-74 21.349999999999998 29.725 25.019999999999996 23.905 75-79 20.825 29.92 26.25 23.005 80-84 22.125 29.395 25.330000000000002 23.150000000000002 85-89 21.240000000000002 29.755 26.029999999999998 22.975 90-94 20.419999999999998 30.630000000000003 25.525 23.425 95-99 20.775 30.099999999999998 25.790000000000003 23.335 100-104 20.435 30.990000000000002 24.905 23.669999999999998 105-109 20.830000000000002 29.07 26.090000000000003 24.01 110-114 20.544999999999998 29.365000000000002 26.88 23.21 115-119 20.45 30.855 24.615000000000002 24.08 120-124 20.075000000000003 30.669999999999998 23.7 25.555 125-129 20.935000000000002 31.135 23.985 23.945 130-134 22.009999999999998 31.115 23.26 23.615 135-139 22.295 30.225 24.62 22.86 140-144 22.17 30.320000000000004 24.38 23.13 145-149 21.54 29.885 24.39 24.185000000000002 150-151 20.4 30.4 24.05 25.15 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.5 2 1.0 3 1.5 4 1.0 5 0.0 6 0.0 7 0.5 8 0.5 9 0.0 10 0.0 11 0.0 12 0.5 13 0.5 14 0.5 15 0.5 16 0.5 17 0.5 18 0.5 19 1.0 20 3.0 21 8.5 22 7.5 23 4.5 24 9.0 25 12.0 26 14.0 27 14.0 28 14.5 29 16.5 30 23.0 31 29.5 32 28.5 33 32.5 34 40.5 35 53.5 36 128.5 37 238.5 38 233.0 39 205.5 40 243.0 41 260.5 42 248.5 43 246.0 44 246.5 45 195.5 46 159.5 47 150.0 48 122.0 49 93.0 50 74.5 51 64.0 52 57.5 53 62.5 54 78.5 55 100.0 56 87.0 57 56.5 58 56.0 59 55.0 60 48.5 61 40.5 62 25.5 63 20.0 64 19.5 65 16.5 66 10.5 67 6.0 68 5.5 69 4.5 70 6.0 71 6.5 72 3.5 73 1.5 74 1.5 75 1.5 76 0.5 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content warn #Base N-Count 1 7.449999999999999 2 0.025 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 73.775 #Duplication Level Percentage of deduplicated Percentage of total 1 86.91968824127414 64.125 2 6.574042697390715 9.700000000000001 3 2.0670958996950186 4.575 4 1.5587936292782107 4.6 5 1.0166045408336157 3.75 6 0.6777363605557438 3.0 7 0.3049813622500847 1.575 8 0.16943409013893596 1.0 9 0.13554727211114878 0.8999999999999999 >10 0.5760759064723823 6.775 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCATTAAT 43 1.075 No Hit TTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCATTAATA 24 0.6 No Hit CTTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCAT 23 0.575 No Hit CGCAGAAGTAGGAATAATAGCACCAGAGATAATATTGTTTCCATAAAGTA 16 0.4 No Hit GGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTACGTT 16 0.4 No Hit TTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCATTA 15 0.375 No Hit CTCATAAGGACCACCATTGTATAACCATTCATCAACGGATGCAGCTTCCC 15 0.375 No Hit GCCAAAATAACCATGAGCAGCCACAATATTATAAGTCTCTTCCTCTTGAC 15 0.375 No Hit TTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCATT 14 0.35000000000000003 No Hit GTTGAAACTAGCATATTGGAAGATTAATCGGCCAAAATAACCATGAGCAG 14 0.35000000000000003 No Hit CGGAAAAACCTTATCCATTAATAGCGGGAACTTCAAGAGCAGCTAGATCT 12 0.3 No Hit GTGCTCTGCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTCCTA 12 0.3 No Hit CTCATTAGCAGATTCATTTTCAGTAGTTTCCCTGATCAAACTAGAGGTTA 12 0.3 No Hit CGGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTACGT 10 0.25 No Hit CCCAAGTATTAATAACGCGACCTTGACTATCAACTACAGATTGGTTGAAA 10 0.25 No Hit CCAGATTCCTACTACAGGCCAAGCAGCCAAGAAGAAGTGTAAAGAACGAG 10 0.25 No Hit GCGGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTACG 10 0.25 No Hit AAAAATTCTTATATGTTAGCGGAAAAACCTTATCCATTAATAGCGGGAAC 9 0.22499999999999998 No Hit ATAAGGACCACCATTGTATAACCATTCATCAACGGATGCAGCTTCCCAAA 9 0.22499999999999998 No Hit GCTACACCTAACATGTGAAATGGATGCATAAGGATGTTGTGCTCTGCCTG 9 0.22499999999999998 No Hit CACCATTGTATAACCATTCATCAACGGATGCAGCTTCCCAAATTGGGTAA 9 0.22499999999999998 No Hit GGCCAAAATAACCATGAGCAGCCACAATATTATAAGTCTCTTCCTCTTGA 8 0.2 No Hit AGATAATATTGTTTCCATAAAGTAAAGAACCAGAAACAGGCTCGCGAATA 8 0.2 No Hit TGATGATATCAGCCCAAGTATTAATAACGCGACCTTGACTATCAACTACA 8 0.2 No Hit CGCATACCCAAACGGAAACTAAGTTCCCACTCACGACCCATATAACAAGC 8 0.2 No Hit GGGAATTCGTAGATCCTCCAGACGTAGAGCACGTAGGGCTTTGAAACCAA 8 0.2 No Hit AGAGAAGCTTCCTTGACCAATAGGGTAAATCAAGAAAACAGCAGTCGCAG 7 0.17500000000000002 No Hit CTTGACTATCAACTACAGATTGGTTGAAATTGAATCCATTTAGGTTGAAC 7 0.17500000000000002 No Hit CAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCATTAATAGCGGGAA 7 0.17500000000000002 No Hit GCCGAAAACACCAGCTACACCTAACATGTGAAATGGATGCATAAGGATGT 7 0.17500000000000002 No Hit CAAGTATTAATAACGCGACCTTGACTATCAACTACAGATTGGTTGAAATT 7 0.17500000000000002 No Hit GGATGCATAAGGATGTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAA 7 0.17500000000000002 No Hit CCACTCACGACCCATATAACAAGCTACACCAAGTAAGAAGTGTAGAACAA 7 0.17500000000000002 No Hit NTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCATTAAT 7 0.17500000000000002 No Hit CGCGACCTTGACTATCAACTACAGATTGGTTGAAATTGAATCCATTTAGG 7 0.17500000000000002 No Hit TCAATATCTACTGGAGGGGCTGCGATGAAGGCGATAATAAATACAGAAGT 6 0.15 No Hit GTATTAATAACGCGACCTTGACTATCAACTACAGATTGGTTGAAATTGAA 6 0.15 No Hit GGAAGATTAATCGGCCAAAATAACCATGAGCAGCCACAATATTATAAGTC 6 0.15 No Hit CTGCGATGAAGGCGATAATAAATACAGAAGTTGCGGTCAATAAGGTAGGG 6 0.15 No Hit CAGAGAAGCTTCCTTGACCAATAGGGTAAATCAAGAAAACAGCAGTCGCA 6 0.15 No Hit GGAAAAACCTTATCCATTAATAGCGGGAACTTCAAGAGCAGCTAGATCTA 6 0.15 No Hit NTTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCAT 6 0.15 No Hit CCATATAACAAGCTACACCAAGTAAGAAGTGTAGAACAATTAGCTCATAA 6 0.15 No Hit GCTAGTTATCCAGTTGCAGAAGCGACCCCACAGGCTTGTACTTTCGCGTC 6 0.15 No Hit GTTCGCTGGAGTAGGCTGCGAGGCCCAAGCGGCCAAGGAGTGCAGCGGCC 6 0.15 No Hit GTTGATGATATCAGCCCAAGTATTAATAACGCGACCTTGACTATCAACTA 6 0.15 No Hit GCTTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCA 6 0.15 No Hit ATATAACAAGCTACACCAAGTAAGAAGTGTAGAACAATTAGCTCATAAGG 6 0.15 No Hit ATCAATATCTACTGGAGGGGCTGCGATGAAGGCGATAATAAATACAGAAG 6 0.15 No Hit AGCGGAAAAACCTTATCCATTAATAGCGGGAACTTCAAGAGCAGCTAGAT 6 0.15 No Hit GGCGGATTTCTCCTTTTGCTCCTCAGCCTACGGGGTATTAGCAACCGTTT 6 0.15 No Hit GATGCATAAGGATGTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAAA 6 0.15 No Hit GCGGAAAAACCTTATCCATTAATAGCGGGAACTTCAAGAGCAGCTAGATC 6 0.15 No Hit GTCAATAAGGTAGGGATCATCAAAACACCGAACCATCCGATGTAAAGACG 6 0.15 No Hit GCTCTGCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTCCTAAA 6 0.15 No Hit CGCGAATACCATCAATATCTACTGGAGGGGCTGCGATGAAGGCGATAATA 5 0.125 No Hit CAGGAACAGGCTCGATGTGATAGCATCGTCCTTTGTAACGATCAAGACTA 5 0.125 No Hit ATCAGAGAAGCTTCCTTGACCAATAGGGTAAATCAAGAAAACAGCAGTCG 5 0.125 No Hit ACCAGATATTCCTAAAGGCATACCATCAGAGAAGCTTCCTTGACCAATAG 5 0.125 No Hit GGCGCATACCCAAACGGAAACTAAGTTCCCACTCACGACCCATATAACAA 5 0.125 No Hit GTCGCAGCTGCAACAGGAGCTGAATATGCAACAGCAATCCAAGGGCGCAT 5 0.125 No Hit GGCGATAATAAATACAGAAGTTGCGGTCAATAAGGTAGGGATCATCAAAA 5 0.125 No Hit GGGAAGTTGTGAGCATTACGTTCGTGCATTACTTCCATACCAAGATTAGC 5 0.125 No Hit TCTTGACCAAATTTGTAACCCTCATTAGCAGATTCATTTTCAGTAGTTTC 5 0.125 No Hit GGATCATCAAAACACCGAACCATCCGATGTAAAGACGATTTTCAGTGCTA 5 0.125 No Hit CATATAACAAGCTACACCAAGTAAGAAGTGTAGAACAATTAGCTCATAAG 5 0.125 No Hit CTAACATGTGAAATGGATGCATAAGGATGTTGTGCTCTGCCTGGAATACA 5 0.125 No Hit GGATGTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAAAGTACCAGAT 5 0.125 No Hit CCAAAATAACCATGAGCAGCCACAATATTATAAGTCTCTTCCTCTTGACC 5 0.125 No Hit CACAATATTATAAGTCTCTTCCTCTTGACCAAATTTGTAACCCTCATTAG 5 0.125 No Hit CTACACCTAACATGTGAAATGGATGCATAAGGATGTTGTGCTCTGCCTGG 5 0.125 No Hit CTCGCTTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTAT 5 0.125 No Hit TGTTGAAACTAGCATATTGGAAGATTAATCGGCCAAAATAACCATGAGCA 5 0.125 No Hit AGATATTCCTAAAGGCATACCATCAGAGAAGCTTCCTTGACCAATAGGGT 5 0.125 No Hit CATAAGGATGTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAAAGTAC 5 0.125 No Hit ACGGAAAAACCTTATCCATTAATAGCGGGAACTTCAAGAGCAGCTAGATC 5 0.125 No Hit GTTGAAATTGAATCCATTTAGGTTGAACGCCATAGTACTAATACCTAAAG 5 0.125 No Hit GCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTCCTAAAGGCAT 5 0.125 No Hit CTCTGCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTCCTAAAG 5 0.125 No Hit GGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTACGTTC 5 0.125 No Hit CCCAGGAACAGGCTCGATGTGATAGCATCGTCCTTTGTAACGATCAAGAC 5 0.125 No Hit CTGGAATACAATCATAAAGTTGAAAGTACCAGATATTCCTAAAGGCATAC 5 0.125 No Hit CCCCACTGCTGCCTCCCGTAGGAGTCTGGGCCGTGTCTCAGTCCCAGTGT 5 0.125 No Hit ATAACAAGCTACACCAAGTAAGAAGTGTAGAACAATTAGCTCATAAGGAC 5 0.125 No Hit CTCGCGAATACCATCAATATCTACTGGAGGGGCTGCGATGAAGGCGATAA 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.025 0.0 0.0 0.0 0.0 66-67 0.025 0.0 0.0 0.0 0.0 68-69 0.025 0.0 0.0 0.0 0.0 70-71 0.025 0.0 0.0 0.0 0.0 72-73 0.05 0.0 0.0 0.0 0.0 74-75 0.0875 0.0 0.0 0.0 0.0 76-77 0.125 0.0 0.0 0.0 0.0 78-79 0.16249999999999998 0.0 0.0 0.0 0.0 80-81 0.25 0.0 0.0 0.0 0.0 82-83 0.2875 0.0 0.0 0.0 0.0 84-85 0.42500000000000004 0.0 0.0 0.0 0.0 86-87 0.4875 0.0 0.0 0.0 0.0 88-89 0.55 0.0 0.0 0.0 0.0 90-91 0.675 0.0 0.0 0.0 0.0 92-93 0.8374999999999999 0.0 0.0 0.0 0.0 94-95 1.0375 0.0 0.0 0.0 0.0 96-97 1.175 0.0 0.0 0.0 0.0 98-99 1.4 0.0 0.0 0.0 0.0 100-101 1.8375 0.0 0.0 0.0 0.0 102-103 2.1875 0.0 0.0 0.0 0.0 104-105 2.65 0.0 0.0 0.0 0.0 106-107 2.9000000000000004 0.0 0.0 0.0 0.0 108-109 3.25 0.0 0.0 0.0 0.0 110-111 3.6500000000000004 0.0 0.0 0.0 0.0 112-113 3.9375 0.0 0.0 0.0 0.0 114-115 4.45 0.0 0.0 0.0 0.0 116-117 5.012499999999999 0.0 0.0 0.0 0.0 118-119 5.575 0.0 0.0 0.0 0.0 120-121 6.1375 0.0 0.0 0.0 0.0 122-123 6.6 0.0 0.0 0.0 0.0 124-125 7.1625 0.0 0.0 0.0 0.0 126-127 7.75 0.0 0.0 0.0 0.0 128-129 8.3625 0.0 0.0 0.0 0.0 130-131 9.3125 0.0 0.0 0.0 0.0 132-133 9.95 0.0 0.0 0.0 0.0 134-135 10.725000000000001 0.0 0.0 0.0 0.0 136-137 11.2375 0.0 0.0 0.0 0.0 138-139 11.7875 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content pass >>END_MODULE SRR6941598 read2 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR6941598_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 33.011 33.0 33.0 34.0 32.0 34.0 2 33.12375 34.0 33.0 34.0 32.0 34.0 3 33.10475 34.0 33.0 34.0 33.0 34.0 4 33.0875 34.0 33.0 34.0 33.0 34.0 5 33.0875 34.0 33.0 34.0 33.0 34.0 6 37.21875 38.0 38.0 38.0 37.0 38.0 7 37.2815 38.0 38.0 38.0 37.0 38.0 8 37.30325 38.0 38.0 38.0 37.0 38.0 9 37.2145 38.0 38.0 38.0 37.0 38.0 10-14 36.96165 38.0 38.0 38.0 36.2 38.0 15-19 37.121849999999995 38.0 38.0 38.0 36.8 38.0 20-24 37.252900000000004 38.0 38.0 38.0 37.0 38.0 25-29 37.188900000000004 38.0 38.0 38.0 37.0 38.0 30-34 36.77804999999999 38.0 38.0 38.0 35.6 38.0 35-39 36.667350000000006 38.0 38.0 38.0 35.0 38.0 40-44 37.084500000000006 38.0 38.0 38.0 36.6 38.0 45-49 37.13645 38.0 38.0 38.0 37.0 38.0 50-54 37.14595 38.0 38.0 38.0 36.8 38.0 55-59 37.022749999999995 38.0 38.0 38.0 36.4 38.0 60-64 37.110049999999994 38.0 38.0 38.0 36.6 38.0 65-69 36.99265 38.0 38.0 38.0 36.2 38.0 70-74 36.716750000000005 38.0 38.0 38.0 35.0 38.0 75-79 36.89335 38.0 38.0 38.0 36.0 38.0 80-84 36.640100000000004 38.0 38.0 38.0 35.2 38.0 85-89 36.43575 38.0 37.8 38.0 34.0 38.0 90-94 36.813599999999994 38.0 38.0 38.0 35.6 38.0 95-99 36.60045 38.0 38.0 38.0 34.6 38.0 100-104 35.72425 38.0 37.0 38.0 30.2 38.0 105-109 36.037349999999996 38.0 37.2 38.0 32.4 38.0 110-114 36.35170000000001 38.0 38.0 38.0 34.0 38.0 115-119 36.33239999999999 38.0 38.0 38.0 34.0 38.0 120-124 35.9486 38.0 37.6 38.0 32.4 38.0 125-129 35.67215 38.0 36.6 38.0 32.0 38.0 130-134 35.7375 38.0 36.8 38.0 32.4 38.0 135-139 32.18805 37.0 27.4 38.0 20.6 38.0 140-144 33.67705 37.4 31.4 38.0 25.8 38.0 145-149 34.626599999999996 38.0 35.6 38.0 29.6 38.0 150-151 30.07125 35.5 28.0 38.0 15.0 38.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 7.0 3 3.0 4 1.0 5 1.0 6 1.0 7 1.0 8 1.0 9 0.0 10 0.0 11 1.0 12 3.0 13 1.0 14 3.0 15 1.0 16 0.0 17 2.0 18 2.0 19 3.0 20 4.0 21 4.0 22 5.0 23 7.0 24 7.0 25 13.0 26 16.0 27 22.0 28 24.0 29 31.0 30 40.0 31 49.0 32 75.0 33 131.0 34 140.0 35 261.0 36 778.0 37 2362.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 35.725 18.15 17.25 28.875 2 27.102102102102105 19.494494494494493 37.212212212212215 16.19119119119119 3 19.97496871088861 23.454317897371716 36.72090112640801 19.849812265331664 4 23.423423423423422 31.406406406406408 25.7007007007007 19.46946946946947 5 23.423423423423422 32.98298298298298 26.25125125125125 17.34234234234234 6 19.975 34.0 28.4 17.625 7 17.25 19.075 43.8 19.875 8 21.5 23.974999999999998 29.425 25.1 9 22.375 20.974999999999998 33.550000000000004 23.1 10-14 24.385 25.755 29.07 20.79 15-19 24.099999999999998 24.965 30.875000000000004 20.06 20-24 24.05 25.28 31.324999999999996 19.345000000000002 25-29 23.9023902390239 25.317531753175317 29.877987798779877 20.9020902090209 30-34 24.24121206060303 25.331266563328164 30.206510325516277 20.22101105055253 35-39 23.875 25.705 29.685 20.735 40-44 23.400000000000002 25.905 29.75 20.945 45-49 23.471173558677936 26.516325816290813 29.951497574878744 20.061003050152507 50-54 23.68618430921546 25.881294064703237 29.736486824341217 20.696034801740087 55-59 22.99 26.195 29.595 21.22 60-64 23.385 25.4 30.464999999999996 20.75 65-69 24.077407740774078 25.59255925592559 29.792979297929794 20.537053705370536 70-74 24.13982796559312 25.91518303660732 29.35587117423485 20.589117823564713 75-79 23.549999999999997 25.840000000000003 30.14 20.47 80-84 24.615000000000002 24.9 30.755 19.73 85-89 24.555 25.724999999999998 29.705 20.015 90-94 23.61 26.115 29.23 21.044999999999998 95-99 23.505000000000003 25.22 30.330000000000002 20.945 100-104 24.345 25.919999999999998 29.38 20.355 105-109 25.115 25.135 29.630000000000003 20.119999999999997 110-114 23.69618480924046 25.76128806440322 30.176508825441275 20.366018300915044 115-119 24.34621731086554 25.061253062653133 29.956497824891244 20.63603180159008 120-124 23.965 26.424999999999997 28.835 20.775 125-129 24.740000000000002 26.375 28.64 20.244999999999997 130-134 24.36 26.3 28.985 20.355 135-139 24.485 26.450000000000003 28.89 20.175 140-144 25.369999999999997 25.5 29.15 19.98 145-149 24.765 25.6 29.5 20.135 150-151 25.224999999999998 25.662499999999998 29.825000000000003 19.287499999999998 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2.0 1 1.5 2 1.0 3 1.0 4 0.5 5 0.0 6 0.0 7 0.0 8 0.0 9 0.5 10 0.5 11 0.5 12 0.5 13 0.0 14 0.0 15 0.0 16 0.0 17 0.5 18 1.0 19 1.5 20 1.5 21 1.5 22 1.5 23 2.0 24 4.0 25 8.0 26 11.0 27 13.0 28 23.0 29 29.5 30 30.5 31 34.5 32 34.0 33 38.0 34 71.0 35 98.0 36 106.0 37 142.0 38 187.0 39 198.5 40 224.0 41 247.5 42 235.5 43 254.0 44 260.5 45 212.0 46 170.0 47 143.0 48 113.0 49 89.5 50 76.5 51 76.5 52 61.5 53 59.0 54 78.0 55 84.0 56 82.0 57 72.0 58 64.0 59 63.5 60 63.5 61 57.5 62 45.0 63 30.5 64 14.0 65 6.5 66 8.5 67 15.5 68 16.5 69 8.0 70 7.0 71 5.5 72 3.5 73 3.0 74 1.0 75 0.5 76 0.5 77 1.0 78 1.5 79 0.5 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.1 3 0.125 4 0.1 5 0.1 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.01 30-34 0.005 35-39 0.0 40-44 0.0 45-49 0.005 50-54 0.005 55-59 0.0 60-64 0.0 65-69 0.01 70-74 0.02 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.005 115-119 0.005 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 71.575 #Duplication Level Percentage of deduplicated Percentage of total 1 84.56164862032833 60.525 2 7.300034928396787 10.45 3 3.108627314006287 6.675000000000001 4 1.8512050296891371 5.3 5 0.9430667132378624 3.375 6 0.5588543485854 2.4 7 0.5239259517988124 2.625 8 0.24449877750611246 1.4000000000000001 9 0.2794271742927 1.7999999999999998 >10 0.6287111421585749 5.45 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source ATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAA 20 0.5 No Hit GTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGATCTT 15 0.375 No Hit GCTTGTTATATGGGTCGTGAGTGGGAACTTAGTTTCCGTTTGGGTATGCG 15 0.375 No Hit TATCTATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTT 14 0.35000000000000003 No Hit ATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCA 13 0.325 No Hit TTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAA 12 0.3 No Hit GTTGCATATTCAGCTCCTGTTGCAGCTGCGACTGCTGTTTTCTTGATTTA 12 0.3 No Hit GCACAACATCCTTATGCATCCATTTCACATGTTAGGTGTAGCTGGTGTTT 12 0.3 No Hit GCTAGTTTCAACAACTCTCGTTCTTTACACTTCTTCTTGGCTGCTTGGCC 11 0.27499999999999997 No Hit GTTGATAGTCAAGGTCGCGTTATTAATACTTGGGCTGATATCATCAACCG 11 0.27499999999999997 No Hit GTGGGAACTTAGTTTCCGTTTGGGTATGCGCCCTTGGATTGCTGTTGCAT 11 0.27499999999999997 No Hit GTTAGGTGTAGCTGGTGTTTTCGGCGGTTCCCTATTCAGTGCTATGCATG 11 0.27499999999999997 No Hit ATTATCTATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAAT 11 0.27499999999999997 No Hit CGCAGCCCCTCCAGTAGATATTGATGGTATTCGCGAGCCTGTTTCTGGTT 10 0.25 No Hit CTATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAA 10 0.25 No Hit GTTATTTTGGCCGATTAATCTTCCAATATGCTAGTTTCAACAACTCTCGT 10 0.25 No Hit GTTCAACCTAAATGGATTCAATTTCAACCAATCTGTAGTTGATAGTCAAG 10 0.25 No Hit GGTACTTTCAACTTTATGATTGTATTCCAGGCAGAGCACAACATCCTTAT 10 0.25 No Hit GTCGTGAGTGGGAACTTAGTTTCCGTTTGGGTATGCGCCCTTGGATTGCT 9 0.22499999999999998 No Hit CCTAAATGGATTCAATTTCAACCAATCTGTAGTTGATAGTCAAGGTCGCG 9 0.22499999999999998 No Hit TATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAAT 9 0.22499999999999998 No Hit ATTTGGTCAAGAGGAAGAGACTTATAATATTGTGGCTGCTCATGGTTATT 9 0.22499999999999998 No Hit CATGACTGCAATTTTAGAGAGACGCGAAAGTACAAGCCTGTGGGGTCGCT 9 0.22499999999999998 No Hit GGAAGCTTCTCTGATGGTATGCCTTTAGGAATATCTGGTACTTTCAACTT 9 0.22499999999999998 No Hit TGATGGTATTCGCGAGCCTGTTTCTGGTTCTTTACTTTATGGAAACAATA 9 0.22499999999999998 No Hit CTTGTTATTGTGAGAATTCTTAATTCAAGAGTTGTAAGGAGGGACTTATG 9 0.22499999999999998 No Hit ATCGGATTGCACTTTTACCCAATTTGGGAAGCTGCATCCGTTGATGAATG 8 0.2 No Hit CTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCTAAATGGAT 8 0.2 No Hit GTTTTGATGATCCCTACCTTATTGACCGCAACTTCTGTATTTATTATCGC 8 0.2 No Hit GTTCGATCCTGGCTCAGGATGAACGCTGGCGGCATGCTTAACACATGCAA 8 0.2 No Hit CAATGGTGGTCCTTATGAGCTAATTGTTCTACACTTCTTACTTGGTGTAG 8 0.2 No Hit GTCAAGGTCGCGTTATTAATACTTGGGCTGATATCATCAACCGTGCTAAT 8 0.2 No Hit GTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGATCTTACCATGACTG 8 0.2 No Hit GGCGTTCAACCTAAATGGATTCAATTTCAACCAATCTGTAGTTGATAGTC 7 0.17500000000000002 No Hit GGTCGCTTCTGCAACTGGATAACTAGCACTGAAAATCGTCTTTACATCGG 7 0.17500000000000002 No Hit TATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAA 7 0.17500000000000002 No Hit CCAAGATCTTACCATGACTGCAATTTTAGAGAGACGCGAAAGTACAAGCC 7 0.17500000000000002 No Hit TGAAAATGAATCTGCTAATGAGGGTTACAAATTTGGTCAAGAGGAAGAGA 7 0.17500000000000002 No Hit AGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGATCT 7 0.17500000000000002 No Hit CTTAGTTTCCGTTTGGGTATGCGCCCTTGGATTGCTGTTGCATATTCAGC 7 0.17500000000000002 No Hit TTCCAATATGCTAGTTTCAACAACTCTCGTTCTTTACACTTCTTCTTGGC 7 0.17500000000000002 No Hit CTGGTGTTTTCGGCGGTTCCCTATTCAGTGCTATGCATGGTTCCTTGGTA 7 0.17500000000000002 No Hit GGGAACTTAGTTTCCGTTTGGGTATGCGCCCTTGGATTGCTGTTGCATAT 7 0.17500000000000002 No Hit GCTGCGACTGCTGTTTTCTTGATTTACCCTATTGGTCAAGGAAGCTTCTC 7 0.17500000000000002 No Hit GTTTGATCAGGGAAACTACTGAAAATGAATCTGCTAATGAGGGTTACAAA 7 0.17500000000000002 No Hit TCCTACTTCTGCGGCAATCGGATTGCACTTTTACCCAATTTGGGAAGCTG 7 0.17500000000000002 No Hit ATCTATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTG 7 0.17500000000000002 No Hit TTCAGCTCCTGTTGCAGCTGCGACTGCTGTTTTCTTGATTTACCCTATTG 7 0.17500000000000002 No Hit CGGCGGTTCCCTATTCAGTGCTATGCATGGTTCCTTGGTAACCTCTAGTT 6 0.15 No Hit TTCACATGTTAGGTGTAGCTGGTGTTTTCGGCGGTTCCCTATTCAGTGCT 6 0.15 No Hit CTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGAT 6 0.15 No Hit GGAAACTACTGAAAATGAATCTGCTAATGAGGGTTACAAATTTGGTCAAG 6 0.15 No Hit CCTGGCTCAGGATGAACGCTGGCGGCATGCTTAACACATGCAAGTCGAAC 6 0.15 No Hit TTTCAACAACTCTCGTTCTTTACACTTCTTCTTGGCTGCTTGGCCTGTAG 6 0.15 No Hit TGGGAACTTAGTTTCCGTTTGGGTATGCGCCCTTGGATTGCTGTTGCATA 6 0.15 No Hit CTTTACTTTATGGAAACAATATTATCTCTGGTGCTATTATTCCTACTTCT 6 0.15 No Hit GTGCTATGCATGGTTCCTTGGTAACCTCTAGTTTGATCAGGGAAACTACT 6 0.15 No Hit ATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCTAAATGG 6 0.15 No Hit GGCTTGCGGTGGATACCTAGGCACCCAGAGACGAGGAAGGGCGTAGCAAG 6 0.15 No Hit TGTAGCTGGTGTTTTCGGCGGTTCCCTATTCAGTGCTATGCATGGTTCCT 6 0.15 No Hit GCTGCATCCGTTGATGAATGGTTATACAATGGTGGTCCTTATGAGCTAAT 6 0.15 No Hit CCTTACCAGGGCTTGACATGCCGCGAATCCTCTTGAAAGAGAGGGGTGCC 6 0.15 No Hit CGGCAATCGGATTGCACTTTTACCCAATTTGGGAAGCTGCATCCGTTGAT 6 0.15 No Hit TCTATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGA 6 0.15 No Hit AGCACAACATCCTTATGCATCCATTTCACATGTTAGGTGTAGCTGGTGTT 5 0.125 No Hit GTAGCTGGTGTTTTCGGCGGTTCCCTATTCAGTGCTATGCATGGTTCCTT 5 0.125 No Hit GGTATTAGTACTATGGCGTTCAACCTAAATGGATTCAATTTCAACCAATC 5 0.125 No Hit CTGCAACTGGATAACTAGCACTGAAAATCGTCTTTACATCGGATGGTTCG 5 0.125 No Hit CTGGTACTTTCAACTTTATGATTGTATTCCAGGCAGAGCACAACATCCTT 5 0.125 No Hit GTCTTTACATCGGATGGTTCGGTGTTTTGATGATCCCTACCTTATTGACC 5 0.125 No Hit ATATCATCAACCGTGCTAATCTTGGTATGGAAGTAATGCACGAACGTAAT 5 0.125 No Hit TGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGATCTTACCATGACT 5 0.125 No Hit ATAATATTGTGGCTGCTCATGGTTATTTTGGCCGATTAATCTTCCAATAT 5 0.125 No Hit CTCTAGTTTGATCAGGGAAACTACTGAAAATGAATCTGCTAATGAGGGTT 5 0.125 No Hit TTCCTACTTCTGCGGCAATCGGATTGCACTTTTACCCAATTTGGGAAGCT 5 0.125 No Hit AATGGATTCAATTTCAACCAATCTGTAGTTGATAGTCAAGGTCGCGTTAT 5 0.125 No Hit CTTGATTTTACCAAAGATGATGAAAACGTAAACTCACAACCATTTATGCG 5 0.125 No Hit ATTATCTCTGGTGCTATTATTCCTACTTCTGCGGCAATCGGATTGCACTT 5 0.125 No Hit GTTATACAATGGTGGTCCTTATGAGCTAATTGTTCTACACTTCTTACTTG 5 0.125 No Hit GGAATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCTAAA 5 0.125 No Hit CAGAAACTAAAGCAAGTGTTGGATTTAAAGCTGGTGTTAAAGATTATAGA 5 0.125 No Hit CAACAACTCTCGTTCTTTACACTTCTTCTTGGCTGCTTGGCCTGTAGTAG 5 0.125 No Hit CGCGAAAGTACAAGCCTGTGGGGTCGCTTCTGCAACTGGATAACTAGCAC 5 0.125 No Hit CATCAACCGTGCTAATCTTGGTATGGAAGTAATGCACGAACGTAATGCTC 5 0.125 No Hit CTCTGGTGCTATTATTCCTACTTCTGCGGCAATCGGATTGCACTTTTACC 5 0.125 No Hit GGATGATCAGCCACACTGGGACTGAGACACGGCCCAGACTCCTACGGGAG 5 0.125 No Hit GAAGAGACTTATAATATTGTGGCTGCTCATGGTTATTTTGGCCGATTAAT 5 0.125 No Hit TGGTTATACAATGGTGGTCCTTATGAGCTAATTGTTCTACACTTCTTACT 5 0.125 No Hit CGCAACTTCTGTATTTATTATCGCCTTCATCGCAGCCCCTCCAGTAGATA 5 0.125 No Hit GTTCTACACTTCTTACTTGGTGTAGCTTGTTATATGGGTCGTGAGTGGGA 5 0.125 No Hit TTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGATCTTA 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.025 0.0 0.0 0.0 0.0 66-67 0.025 0.0 0.0 0.0 0.0 68-69 0.025 0.0 0.0 0.0 0.0 70-71 0.025 0.0 0.0 0.0 0.0 72-73 0.05 0.0 0.0 0.0 0.0 74-75 0.0875 0.0 0.0 0.0 0.0 76-77 0.125 0.0 0.0 0.0 0.0 78-79 0.16249999999999998 0.0 0.0 0.0 0.0 80-81 0.25 0.0 0.0 0.0 0.0 82-83 0.2875 0.0 0.0 0.0 0.0 84-85 0.42500000000000004 0.0 0.0 0.0 0.0 86-87 0.4875 0.0 0.0 0.0 0.0 88-89 0.55 0.0 0.0 0.0 0.0 90-91 0.675 0.0 0.0 0.0 0.0 92-93 0.8374999999999999 0.0 0.0 0.0 0.0 94-95 0.9875 0.0 0.0 0.0 0.0 96-97 1.0875 0.0 0.0 0.0 0.0 98-99 1.2875 0.0 0.0 0.0 0.0 100-101 1.65 0.0 0.0 0.0 0.0 102-103 1.9625000000000001 0.0 0.0 0.0 0.0 104-105 2.4 0.0 0.0 0.0 0.0 106-107 2.6500000000000004 0.0 0.0 0.0 0.0 108-109 3.0 0.0 0.0 0.0 0.0 110-111 3.4000000000000004 0.0 0.0 0.0 0.0 112-113 3.6875 0.0 0.0 0.0 0.0 114-115 4.225 0.0 0.0 0.0 0.0 116-117 4.8125 0.0 0.0 0.0 0.0 118-119 5.387499999999999 0.0 0.0 0.0 0.0 120-121 5.949999999999999 0.0 0.0 0.0 0.0 122-123 6.425 0.0 0.0 0.0 0.0 124-125 6.949999999999999 0.0 0.0 0.0 0.0 126-127 7.449999999999999 0.0 0.0 0.0 0.0 128-129 7.95 0.0 0.0 0.0 0.0 130-131 8.6 0.0 0.0 0.0 0.0 132-133 9.1375 0.0 0.0 0.0 0.0 134-135 9.75 0.0 0.0 0.0 0.0 136-137 10.175 0.0 0.0 0.0 0.0 138-139 10.587499999999999 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGTGTCT 10 0.006830828 145.0 145 GTGCTAT 45 0.008957279 48.333332 6 GCTGGCC 20 0.00593511 29.0 85-89 >>END_MODULE Read 1149794 spots for SRR6941598.sra Written 1149794 spots for SRR6941598.sra Read 1149794 spots for SRR6941598.sra Written 1149794 spots for SRR6941598.sra Read 1149794 spots for SRR6941598.sra Written 1149794 spots for SRR6941598.sra Read 1149794 spots for SRR6941598.sra Written 1149794 spots for SRR6941598.sra Read 1149794 spots for SRR6941598.sra Written 1149794 spots for SRR6941598.sra Read 1149794 spots for SRR6941598.sra Written 1149794 spots for SRR6941598.sra Read 1149794 spots for SRR6941598.sra Written 1149794 spots for SRR6941598.sra Read 1149794 spots for SRR6941598.sra Written 1149794 spots for SRR6941598.sra Read 1149794 spots for SRR6941598.sra Written 1149794 spots for SRR6941598.sra Read 1149794 spots for SRR6941598.sra Written 1149794 spots for SRR6941598.sra Read 1149794 spots for SRR6941598.sra Written 1149794 spots for SRR6941598.sra Read 1149794 spots for SRR6941598.sra Written 1149794 spots for SRR6941598.sra Read 1149794 spots for SRR6941598.sra Written 1149794 spots for SRR6941598.sra Read 1149794 spots for SRR6941598.sra Written 1149794 spots for SRR6941598.sra Read 1149813 spots for SRR6941598.sra Written 1149813 spots for SRR6941598.sra Read 1149794 spots for SRR6941598.sra Written 1149794 spots for SRR6941598.sra Read 1149794 spots for SRR6941598.sra Written 1149794 spots for SRR6941598.sra Read 1149794 spots for SRR6941598.sra Written 1149794 spots for SRR6941598.sra Read 1149794 spots for SRR6941598.sra Written 1149794 spots for SRR6941598.sra Read 1149794 spots for SRR6941598.sra Written 1149794 spots for SRR6941598.sra SRR ids: ['SRR6941598.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_3tymhfo2 SRR6941598.sra spots: 22995899 blocks: [[1, 1149794], [1149795, 2299588], [2299589, 3449382], [3449383, 4599176], [4599177, 5748970], [5748971, 6898764], [6898765, 8048558], [8048559, 9198352], [9198353, 10348146], [10348147, 11497940], [11497941, 12647734], [12647735, 13797528], [13797529, 14947322], [14947323, 16097116], [16097117, 17246910], [17246911, 18396704], [18396705, 19546498], [19546499, 20696292], [20696293, 21846086], [21846087, 22995899]] SRR6941598 file size 7770855 SRR6941598 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR6941598 SRR6941598_1.fastq SRR6941598_2.fastq Input file: SRR6941598_1.fastq Paired file: SRR6941598_2.fastq trimmed: SRR6941598-trimmed-pair1.fastq, SRR6941598-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Fri Dec 6 12:44:00 2024 >> started Fri Dec 6 12:44:32 2024 >> done (31.880s) 22995899 read pairs processed; of these: 24440 ( 0.11%) short read pairs filtered out after trimming by size control 20899 ( 0.09%) empty read pairs filtered out after trimming by size control 22950560 (99.80%) read pairs available; of these: 9464389 (41.24%) trimmed read pairs available after processing 13486171 (58.76%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 2 0.00% 19 1 0.00% 20 4 0.00% 21 4 0.00% 22 2 0.00% 23 2 0.00% 24 10 0.00% 25 7 0.00% 26 6 0.00% 27 30 0.00% 28 4 0.00% 29 2 0.00% 30 9 0.00% 31 4 0.00% 32 9 0.00% 33 2 0.00% 34 4 0.00% 35 4 0.00% 36 5 0.00% 37 12 0.00% 38 6 0.00% 39 17 0.00% 40 15 0.00% 41 19 0.00% 42 21 0.00% 43 40 0.00% 44 30 0.00% 45 54 0.00% 46 29 0.00% 47 45 0.00% 48 55 0.00% 49 68 0.00% 50 87 0.00% 51 98 0.00% 52 132 0.00% 53 153 0.00% 54 167 0.00% 55 206 0.00% 56 208 0.00% 57 208 0.00% 58 254 0.00% 59 345 0.00% 60 364 0.00% 61 468 0.00% 62 542 0.00% 63 709 0.00% 64 771 0.00% 65 842 0.00% 66 980 0.00% 67 1052 0.00% 68 1161 0.01% 69 1369 0.01% 70 1670 0.01% 71 1893 0.01% 72 2423 0.01% 73 2611 0.01% 74 3092 0.01% 75 3529 0.02% 76 3773 0.02% 77 4319 0.02% 78 4559 0.02% 79 5425 0.02% 80 6117 0.03% 81 7066 0.03% 82 8030 0.03% 83 8530 0.04% 84 10516 0.05% 85 12614 0.05% 86 13068 0.06% 87 14414 0.06% 88 16254 0.07% 89 16901 0.07% 90 18226 0.08% 91 19147 0.08% 92 22400 0.10% 93 23360 0.10% 94 24801 0.11% 95 27770 0.12% 96 27013 0.12% 97 29427 0.13% 98 31778 0.14% 99 33618 0.15% 100 33947 0.15% 101 36221 0.16% 102 39110 0.17% 103 39227 0.17% 104 42868 0.19% 105 45187 0.20% 106 46159 0.20% 107 48000 0.21% 108 49047 0.21% 109 51245 0.22% 110 51806 0.23% 111 55155 0.24% 112 55663 0.24% 113 56868 0.25% 114 62002 0.27% 115 66420 0.29% 116 69711 0.30% 117 70539 0.31% 118 68294 0.30% 119 68441 0.30% 120 70562 0.31% 121 73706 0.32% 122 80926 0.35% 123 80314 0.35% 124 83586 0.36% 125 90810 0.40% 126 83801 0.37% 127 88335 0.38% 128 90183 0.39% 129 93491 0.41% 130 87772 0.38% 131 93611 0.41% 132 97669 0.43% 133 95571 0.42% 134 103242 0.45% 135 101628 0.44% 136 103135 0.45% 137 104095 0.45% 138 112624 0.49% 139 115405 0.50% 140 117073 0.51% 141 135317 0.59% 142 129856 0.57% 143 141294 0.62% 144 151904 0.66% 145 177780 0.77% 146 195412 0.85% 147 236855 1.03% 148 336990 1.47% 149 608433 2.65% 150 3912117 17.05% 151 13486171 58.76% 22950560 reads passed initial QC criterion=sequence-density sequence-density=0.40 sequence-density-rank=1 fanout-score=1.96 fanout-score-rank=34 prefix-density=0.40 prefix-fanout=2.0 sequence=CCACTACGCAACTTGGAACGGGCGGGCCATCAGTAGCACACCTAGACCAGGCCCGCAGCTCTACAGCAACGTCCACACCACCCTTAAAGCCCCCACTCGGGTTACAAGCTCTCGCTA criterion=fanout-score sequence-density=0.01 sequence-density-rank=39 fanout-score=97.77 fanout-score-rank=1 prefix-density=0.33 prefix-fanout=3.0 sequence=AGAAAAAAACAAGTTTGCATCTTCAGGAGAATCTATATTTTCGCGAAATGGATCATAATAAAATGGATTTTAGGTATCTAGGGAAAATTCACTTCGAAGTAACTATTTCCTAGATACCTATGCACGGTACTTCACGGTTGAATGAATCAACCTGAAAAATACCTAAAAAAGGCCTAAAGTTAAGGATTTATCAATGGGTAATGTTGCTCCAATACCTAACCAAAGAGCTACTGCAGTACCGATTAAAAAAACGGTCGTAGCTACTGGGCGACGAAATGGATTTTGGAATTTGTTGACATTCTCTAGAAAAGGTACTGTCAATAAGCCTGTTGGCACAGAAACCATTAAGAGAACGCCCAATAACTTATTGGGTACCGTACGGAGTATTTGAAACACGGGAAAGAAGTACCACTCGGGTAATATTTCCAAAGGAGTTGCAAACGGATCCGCGGGTTCACCAATCATTGATGGCTCGAGAACAGCTAAACCTACATTACATGCAATAGTACCT criterion=sequence-density sequence-density=0.40 sequence-density-rank=1 fanout-score=2.62 fanout-score-rank=22 prefix-density=1.05 prefix-fanout=1.0 sequence=TTGCGTAGTGGATCTGCTGGGGCCTATGCGAAAGCTGGGCCTCACGGATCCTAGAGCGGCAGGCACCGCGTGAGGCTG criterion=fanout-score sequence-density=0.04 sequence-density-rank=26 fanout-score=62.67 fanout-score-rank=1 prefix-density=0.99 prefix-fanout=2.7 sequence=GGTGGAAGGATCAGGTGCATGCAGGTGTGGCCGATCGAGGGCATCAAGAAGTTCGAGACCCTCTCGTACCTGCCCCCTCTCTCCGTGGAGTCTCTCCTGAAGCAGATCGAGTACCTGATCCGCTCCAAGTGGGTTCCTTGCCT SRR6941598 testing PE reads STAR mapping to Ensembl genome Started job on | Dec 06 12:45:55 Started mapping on | Dec 06 12:45:56 Finished on | Dec 06 12:48:50 Mapping speed, Million of reads per hour | 474.84 Number of input reads | 22950560 Average input read length | 291 UNIQUE READS: Uniquely mapped reads number | 13553887 Uniquely mapped reads % | 59.06% Average mapped length | 293.36 Number of splices: Total | 2795808 Number of splices: Annotated (sjdb) | 2532574 Number of splices: GT/AG | 2672373 Number of splices: GC/AG | 31739 Number of splices: AT/AC | 10730 Number of splices: Non-canonical | 80966 Mismatch rate per base, % | 0.31% Deletion rate per base | 0.02% Deletion average length | 1.96 Insertion rate per base | 0.02% Insertion average length | 2.40 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 8076560 % of reads mapped to multiple loci | 35.19% Number of reads mapped to too many loci | 67985 % of reads mapped to too many loci | 0.30% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 3.92% % of reads unmapped: other | 1.53% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 1330148 1330148 1330148 N_multimapping 8076560 8076560 8076560 N_noFeature 5384317 13060152 5576228 N_ambiguous 555982 8578 261268 UnstrandedReadsAssigned:7613588 PositiveStrandReadsAssigned:485157 NegativeStrandReadsAssigned:7716391 Dataset is classified negative stranded MeadianReadLen=151 20thPercentileLength=149 echo kmer=145 SRR6941598 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,972 [index] number of k-mers: 66,720,672 [index] number of equivalence classes: 111,837 [quant] running in paired-end mode [quant] will process pair 1: SRR6941598-trimmed-pair1.fastq SRR6941598-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 22,950,560 reads, 12,653,594 reads pseudoaligned [quant] estimated average fragment length: 218.88 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,042 rounds 52973 SRR6941598.ke.tsv 35125 SRR6941598.se.tsv 88098 total ==> SRR6941598.ke.tsv <== target_id length eff_length est_counts tpm PNS24245 936 718.685 0 0 PNS24247 1044 826.12 7.05148 0.649027 PNS24249 1928 1710.12 12.3065 0.547183 PNS24246 1044 826.12 7.05148 0.649027 PNS24248 1044 826.12 7.05148 0.649027 PNS24244 1471 1253.12 10.5391 0.639492 PNS24243 293 111.458 0 0 KQK14069 1603 1385.12 442.655 24.2999 KQK14071 474 265.961 5.83841 1.66918 ==> SRR6941598.se.tsv <== BRADI_1g14170v3 535 BRADI_1g53295v3 47 BRADI_1g59795v3 9 BRADI_1g07683v3 0 BRADI_1g00485v3 0 BRADI_1g20270v3 80 BRADI_1g74790v3 21 BRADI_1g09890v3 0 BRADI_1g77505v3 34 BRADI_1g48960v3 0 SRR6941598 completed mapping pipeline successfully