Starting /dee2/code/volunteer_pipeline.sh SRR6941599 current disk space = 1551341486080 free memory = 1603755628 SRR6941599 SRAfilesize 03a6d1129f87c3d33f0119d83ef03bd4 SRR6941599.sra SRR6941599.sra file validated SRR6941599 is paired end SRR6941599 is conventional basespace SRR6941599 read1 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR6941599_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.6705 34.0 33.0 34.0 32.0 34.0 2 33.123 34.0 33.0 34.0 32.0 34.0 3 33.2445 34.0 33.0 34.0 32.0 34.0 4 33.36075 34.0 33.0 34.0 33.0 34.0 5 33.4305 34.0 33.0 34.0 33.0 34.0 6 37.27875 38.0 38.0 38.0 36.0 38.0 7 37.576 38.0 38.0 38.0 37.0 38.0 8 37.6105 38.0 38.0 38.0 38.0 38.0 9 37.6545 38.0 38.0 38.0 38.0 38.0 10-14 37.65310000000001 38.0 38.0 38.0 38.0 38.0 15-19 37.6654 38.0 38.0 38.0 38.0 38.0 20-24 37.61325 38.0 38.0 38.0 38.0 38.0 25-29 37.57755 38.0 38.0 38.0 38.0 38.0 30-34 37.4694 38.0 38.0 38.0 38.0 38.0 35-39 37.49425 38.0 38.0 38.0 37.8 38.0 40-44 37.525 38.0 38.0 38.0 38.0 38.0 45-49 37.53335 38.0 38.0 38.0 38.0 38.0 50-54 37.51125 38.0 38.0 38.0 38.0 38.0 55-59 37.482749999999996 38.0 38.0 38.0 38.0 38.0 60-64 37.427400000000006 38.0 38.0 38.0 37.6 38.0 65-69 37.30835 38.0 38.0 38.0 37.0 38.0 70-74 37.2918 38.0 38.0 38.0 37.0 38.0 75-79 37.3392 38.0 38.0 38.0 37.0 38.0 80-84 37.300200000000004 38.0 38.0 38.0 37.0 38.0 85-89 37.048700000000004 38.0 38.0 38.0 36.4 38.0 90-94 37.110699999999994 38.0 38.0 38.0 36.4 38.0 95-99 36.9467 38.0 38.0 38.0 35.8 38.0 100-104 37.0207 38.0 38.0 38.0 36.0 38.0 105-109 36.8029 38.0 38.0 38.0 35.0 38.0 110-114 36.31609999999999 38.0 38.0 38.0 34.2 38.0 115-119 36.38575 38.0 38.0 38.0 34.0 38.0 120-124 36.41395 38.0 38.0 38.0 34.0 38.0 125-129 36.47035 38.0 38.0 38.0 34.2 38.0 130-134 36.2603 38.0 38.0 38.0 33.8 38.0 135-139 35.98675000000001 38.0 37.6 38.0 33.2 38.0 140-144 35.69955 38.0 36.0 38.0 32.4 38.0 145-149 35.23125 38.0 36.0 38.0 31.0 38.0 150-151 31.026875 35.5 30.0 38.0 15.0 38.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 1.0 10 0.0 11 0.0 12 0.0 13 0.0 14 1.0 15 1.0 16 2.0 17 0.0 18 0.0 19 5.0 20 2.0 21 1.0 22 6.0 23 3.0 24 4.0 25 4.0 26 7.0 27 10.0 28 19.0 29 26.0 30 36.0 31 32.0 32 51.0 33 69.0 34 106.0 35 207.0 36 400.0 37 3006.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.6270470153196 11.753829899630217 7.131537242472266 39.48758584257792 2 23.523523523523522 13.913913913913914 34.68468468468468 27.87787787787788 3 21.0 19.900000000000002 24.825 34.275 4 25.3 28.749999999999996 21.6 24.349999999999998 5 23.3 32.574999999999996 23.325000000000003 20.8 6 19.525000000000002 33.6 23.974999999999998 22.900000000000002 7 16.025 23.5 40.525 19.950000000000003 8 20.1 21.6 29.625 28.675 9 18.325 21.65 31.85 28.175 10-14 22.55 26.840000000000003 24.27 26.340000000000003 15-19 22.485 25.5 26.255 25.759999999999998 20-24 22.48 25.995 25.629999999999995 25.895000000000003 25-29 22.830000000000002 25.39 26.200000000000003 25.580000000000002 30-34 22.155 26.224999999999998 26.279999999999998 25.34 35-39 21.81327199079862 25.94389158373756 26.43396509476422 25.808871330699606 40-44 22.559023609443777 25.175070028011206 26.50560224089636 25.76030412164866 45-49 20.919999999999998 25.790000000000003 27.41 25.88 50-54 21.79480766344855 25.496473413035865 25.896653494072332 26.812065429443248 55-59 21.586475942782833 26.32289686906072 25.682704811443436 26.407922376713017 60-64 21.358203730559584 25.62384357653648 26.82402360354053 26.193929089363404 65-69 22.065 25.569999999999997 25.564999999999998 26.8 70-74 22.78 25.814999999999998 24.705 26.700000000000003 75-79 22.085 26.650000000000002 25.119999999999997 26.145000000000003 80-84 23.080000000000002 25.655 25.585 25.679999999999996 85-89 22.065 24.93 26.58 26.424999999999997 90-94 22.28222822282228 26.022602260226023 24.987498749874987 26.707670767076706 95-99 21.935 25.919999999999998 25.465 26.68 100-104 22.268907563025213 26.58563425370148 24.59483793517407 26.55062024809924 105-109 22.725 25.52 25.91 25.845000000000002 110-114 22.94025869444864 25.678192158639085 26.196587649101616 25.184961497810658 115-119 22.407388816383897 26.643911253890174 25.10792089147676 25.840779038249174 120-124 21.9923870579986 26.94580787338475 23.76039266753481 27.301412401081837 125-129 22.71181354406322 26.958087426227866 23.91217365209563 26.417925377613283 130-134 23.477043112933877 26.59297789336801 23.60708212463739 26.32289686906072 135-139 23.200000000000003 27.615000000000002 23.965 25.22 140-144 23.185 26.775 24.005000000000003 26.035000000000004 145-149 23.150000000000002 26.93 24.185000000000002 25.735000000000003 150-151 21.8125 27.125 24.2875 26.775 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.5 2 0.5 3 0.0 4 0.0 5 0.5 6 0.5 7 0.5 8 1.0 9 0.5 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.5 18 1.0 19 0.5 20 0.0 21 2.0 22 4.0 23 2.5 24 3.0 25 6.5 26 7.0 27 9.0 28 14.0 29 16.0 30 18.0 31 19.0 32 20.5 33 26.0 34 33.0 35 44.5 36 81.5 37 137.0 38 133.5 39 105.5 40 145.5 41 166.0 42 134.5 43 137.0 44 136.0 45 124.5 46 115.5 47 106.0 48 108.5 49 100.5 50 108.5 51 121.5 52 121.0 53 140.0 54 179.5 55 227.5 56 226.0 57 190.5 58 180.0 59 147.0 60 94.0 61 65.5 62 59.5 63 37.0 64 21.5 65 22.5 66 15.0 67 9.0 68 7.0 69 7.5 70 9.0 71 10.5 72 9.5 73 8.0 74 7.0 75 7.0 76 4.5 77 1.0 78 0.5 79 0.5 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content warn #Base N-Count 1 5.35 2 0.1 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.015 40-44 0.04 45-49 0.0 50-54 0.045 55-59 0.03 60-64 0.015 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.01 95-99 0.0 100-104 0.04 105-109 0.0 110-114 0.655 115-119 0.38999999999999996 120-124 0.16999999999999998 125-129 0.03 130-134 0.03 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 73.625 #Duplication Level Percentage of deduplicated Percentage of total 1 83.5314091680815 61.5 2 8.828522920203735 13.0 3 3.9728353140916806 8.774999999999999 4 1.4601018675721562 4.3 5 0.7809847198641765 2.875 6 0.5093378607809848 2.25 7 0.27164685908319186 1.4000000000000001 8 0.27164685908319186 1.6 9 0.0 0.0 >10 0.37351443123938877 4.3 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCATTAAT 37 0.9249999999999999 No Hit GTACAAGGCCCGGGAACGGATTCACCGCCGTATGGCTGACCGGCGATTAC 21 0.525 No Hit GCGGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTACG 16 0.4 No Hit GGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTACGTT 16 0.4 No Hit GCCAAAATAACCATGAGCAGCCACAATATTATAAGTCTCTTCCTCTTGAC 14 0.35000000000000003 No Hit CGCAGAAGTAGGAATAATAGCACCAGAGATAATATTGTTTCCATAAAGTA 13 0.325 No Hit CATATAACAAGCTACACCAAGTAAGAAGTGTAGAACAATTAGCTCATAAG 12 0.3 No Hit GTTGAAACTAGCATATTGGAAGATTAATCGGCCAAAATAACCATGAGCAG 12 0.3 No Hit ACATGCTCCACCGCTTGTGCGGGCCCCCGTCAATTCCTTTGAGTTTCATT 11 0.27499999999999997 No Hit CCCGAAGTTACGGGGCTATTTTGCCGAGTTCCTTAGAGAGAGTTGTCTCG 10 0.25 No Hit GCGGAAAAACCTTATCCATTAATAGCGGGAACTTCAAGAGCAGCTAGATC 10 0.25 No Hit GGGCGGTGTGTACAAGGCCCGGGAACGGATTCACCGCCGTATGGCTGACC 8 0.2 No Hit CACATGCTCCACCGCTTGTGCGGGCCCCCGTCAATTCCTTTGAGTTTCAT 8 0.2 No Hit CCCAAGTATTAATAACGCGACCTTGACTATCAACTACAGATTGGTTGAAA 8 0.2 No Hit GCCAGCTCCTATAGTGTGACGGGCGGTGTGTACAAGGCCCGGGAACGGAT 8 0.2 No Hit GGGCTTACTACTTATATGCTTTCAGCAGTTATCCTCTCCGCACTTGGCTA 8 0.2 No Hit GTGAAATGGATGCATAAGGATGTTGTGCTCTGCCTGGAATACAATCATAA 8 0.2 No Hit CTTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCAT 8 0.2 No Hit CTCGCGAATACCATCAATATCTACTGGAGGGGCTGCGATGAAGGCGATAA 8 0.2 No Hit CCTAGATGTCCAGTCAACTGCTGCGCCTCAACGCATTTCGGGGAGAACCA 7 0.17500000000000002 No Hit GCTCCACCGCTTGTGCGGGCCCCCGTCAATTCCTTTGAGTTTCATTCTTG 7 0.17500000000000002 No Hit GGGATCATCAAAACACCGAACCATCCGATGTAAAGACGATTTTCAGTGCT 7 0.17500000000000002 No Hit GTTTCTTCTCCGAGAAAAGAAGTTGACGACCCGTAGGCCTTCCACCTCCA 7 0.17500000000000002 No Hit GCATAAGGATGTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAAAGTA 7 0.17500000000000002 No Hit GTGCGACGTGGGGCTGGATCTCAGTGGATCGTGGCAGCAAGGCCACTCTG 7 0.17500000000000002 No Hit GGTGTGTACAAGGCCCGGGAACGGATTCACCGCCGTATGGCTGACCGGCG 7 0.17500000000000002 No Hit GGTAAATCAAGAAAACAGCAGTCGCAGCTGCAACAGGAGCTGAATATGCA 7 0.17500000000000002 No Hit CACCGCTTGTGCGGGCCCCCGTCAATTCCTTTGAGTTTCATTCTTGCGAA 6 0.15 No Hit CTCCACCGCTTGTGCGGGCCCCCGTCAATTCCTTTGAGTTTCATTCTTGC 6 0.15 No Hit GTGTACAAGGCCCGGGAACGGATTCACCGCCGTATGGCTGACCGGCGATT 6 0.15 No Hit GTCGGTTTCGGGTACAGGTACCCTTTTGTTGAAGGTCGTTCGAGCTTTTC 6 0.15 No Hit TTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCATT 6 0.15 No Hit GCCGACTTTCGTCTCTGCTCGACGGGTGAGTCTTGCAGTCAAGCTCCCTT 6 0.15 No Hit GTGAGCATTACGTTCGTGCATTACTTCCATACCAAGATTAGCACGGTTGA 6 0.15 No Hit CTTGGGCGGATTTCTCCTTTTGCTCCTCAGCCTACGGGGTATTAGCAACC 6 0.15 No Hit GTGTGTACAAGGCCCGGGAACGGATTCACCGCCGTATGGCTGACCGGCGA 6 0.15 No Hit GTTCGCTGGAGTAGGCTGCGAGGCCCAAGCGGCCAAGGAGTGCAGCGGCC 6 0.15 No Hit CTCATAAGGACCACCATTGTATAACCATTCATCAACGGATGCAGCTTCCC 6 0.15 No Hit CTTGACCAAATTTGTAACCCTCATTAGCAGATTCATTTTCAGTAGTTTCC 6 0.15 No Hit AGCGGAAAAACCTTATCCATTAATAGCGGGAACTTCAAGAGCAGCTAGAT 6 0.15 No Hit GTCGGTTCGGACCTCTGCTTAGTTTCATCCAAGCTTCATCCTGGTCATGG 6 0.15 No Hit ACCACATGCTCCACCGCTTGTGCGGGCCCCCGTCAATTCCTTTGAGTTTC 6 0.15 No Hit AGAGAAGCTTCCTTGACCAATAGGGTAAATCAAGAAAACAGCAGTCGCAG 5 0.125 No Hit GGCCAAAATAACCATGAGCAGCCACAATATTATAAGTCTCTTCCTCTTGA 5 0.125 No Hit GGAAGATTAATCGGCCAAAATAACCATGAGCAGCCACAATATTATAAGTC 5 0.125 No Hit ACCGCTTGTGCGGGCCCCCGTCAATTCCTTTGAGTTTCATTCTTGCGAAC 5 0.125 No Hit GGCATGATGACTTGGCCTCATCCTCTCCTTCCTCCGGCTTAACACCGGCG 5 0.125 No Hit CTCCCATTTCGCTCGCCGCTACTACGGGAATCGCTTTTGCTTTCTTTTCC 5 0.125 No Hit GCCACCTACAGACGCTTTACGCCCAATCATTCCGGATAACGCTTGCATCC 5 0.125 No Hit GGATGCATAAGGATGTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAA 5 0.125 No Hit CACTCATCTTGGGGTGGGCTTACTACTTATATGCTTTCAGCAGTTATCCT 5 0.125 No Hit TTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCATTAATA 5 0.125 No Hit GGCTGATCATCCTCTCGGACCAGCTACTGATCATCGCCTTGGTAAGCTAT 5 0.125 No Hit CGGAAAAACCTTATCCATTAATAGCGGGAACTTCAAGAGCAGCTAGATCT 5 0.125 No Hit GTGCTCTGCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTCCTA 5 0.125 No Hit GCTAATCAGACGCGAGCCCCTCCTTGGGCGGATTTCTCCTTTTGCTCCTC 5 0.125 No Hit GCCCAATCATTCCGGATAACGCTTGCATCCTCTGTCTTACCGCGGCTGCT 5 0.125 No Hit CTCCAGTCGCAAGCCTAGCCTTAGGCATCCCCCTCCTTACGGTTAAGGGT 5 0.125 No Hit GAAAAACCTTATCCATTAATAGCGGGAACTTCAAGAGCAGCTAGATCTAG 5 0.125 No Hit GGGAATTCGTAGATCCTCCAGACGTAGAGCACGTAGGGCTTTGAAACCAA 5 0.125 No Hit CTTCACTCCAGTCGCAAGCCTAGCCTTAGGCATCCCCCTCCTTACGGTTA 5 0.125 No Hit TACAAGGCCCGGGAACGGATTCACCGCCGTATGGCTGACCGGCGATTACT 5 0.125 No Hit CACCATTGTATAACCATTCATCAACGGATGCAGCTTCCCAAATTGGGTAA 5 0.125 No Hit CCCCACTGCTGCCTCCCGTAGGAGTCTGGGCCGTGTCTCAGTCCCAGTGT 5 0.125 No Hit CTTTCTTTTCCTCTGGCTACTAAGATGTTTCAGTTCGCCAGGTTGTCTCT 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.025 0.0 0.0 0.0 0.0 66-67 0.05 0.0 0.0 0.0 0.0 68-69 0.05 0.0 0.0 0.0 0.0 70-71 0.05 0.0 0.0 0.0 0.0 72-73 0.0625 0.0 0.0 0.0 0.0 74-75 0.075 0.0 0.0 0.0 0.0 76-77 0.075 0.0 0.0 0.0 0.0 78-79 0.1125 0.0 0.0 0.0 0.0 80-81 0.1375 0.0 0.0 0.0 0.0 82-83 0.175 0.0 0.0 0.0 0.0 84-85 0.2375 0.0 0.0 0.0 0.0 86-87 0.2875 0.0 0.0 0.0 0.0 88-89 0.32499999999999996 0.0 0.0 0.0 0.0 90-91 0.4375 0.0 0.0 0.0 0.0 92-93 0.6625000000000001 0.0 0.0 0.0 0.0 94-95 0.7375 0.0 0.0 0.0 0.0 96-97 0.9125000000000001 0.0 0.0 0.0 0.0 98-99 1.15 0.0 0.0 0.0 0.0 100-101 1.3625 0.0 0.0 0.0 0.0 102-103 1.6625 0.0 0.0 0.0 0.0 104-105 1.9625 0.0 0.0 0.0 0.0 106-107 2.2125 0.0 0.0 0.0 0.0 108-109 2.725 0.0 0.0 0.0 0.0 110-111 3.225 0.0 0.0 0.0 0.0 112-113 3.8375000000000004 0.0 0.0 0.0 0.0 114-115 4.275 0.0 0.0 0.0 0.0 116-117 4.775 0.0 0.0 0.0 0.0 118-119 5.35 0.0 0.0 0.0 0.0 120-121 5.975 0.0 0.0 0.0 0.0 122-123 6.7375 0.0 0.0 0.0 0.0 124-125 7.5125 0.0 0.0 0.0 0.0 126-127 8.2375 0.0 0.0 0.0 0.0 128-129 9.075 0.0 0.0 0.0 0.0 130-131 9.662500000000001 0.0 0.0 0.0 0.0 132-133 10.287500000000001 0.0 0.0 0.0 0.0 134-135 11.1125 0.0 0.0 0.0 0.0 136-137 11.9375 0.0 0.0 0.0 0.0 138-139 12.4625 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CACGAGA 10 0.0060887975 150.61038 1 GAGAACA 10 0.006836113 144.9625 4 ACGAGAA 10 0.006836113 144.9625 2 AGCGCTG 10 0.006836113 144.9625 145 CGAGAAC 10 0.006836113 144.9625 3 GATCGGA 45 0.008966441 48.320835 145 >>END_MODULE SRR6941599 read2 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR6941599_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 33.189 34.0 33.0 34.0 33.0 34.0 2 33.25575 34.0 33.0 34.0 33.0 34.0 3 33.271 34.0 33.0 34.0 33.0 34.0 4 33.32175 34.0 33.0 34.0 33.0 34.0 5 33.27075 34.0 33.0 34.0 33.0 34.0 6 37.48025 38.0 38.0 38.0 38.0 38.0 7 37.49425 38.0 38.0 38.0 38.0 38.0 8 37.421 38.0 38.0 38.0 38.0 38.0 9 37.42825 38.0 38.0 38.0 38.0 38.0 10-14 37.41995 38.0 38.0 38.0 38.0 38.0 15-19 37.424549999999996 38.0 38.0 38.0 38.0 38.0 20-24 37.393049999999995 38.0 38.0 38.0 38.0 38.0 25-29 37.308350000000004 38.0 38.0 38.0 37.4 38.0 30-34 37.35594999999999 38.0 38.0 38.0 38.0 38.0 35-39 37.33155 38.0 38.0 38.0 37.8 38.0 40-44 37.306599999999996 38.0 38.0 38.0 37.6 38.0 45-49 37.2922 38.0 38.0 38.0 37.2 38.0 50-54 37.30715 38.0 38.0 38.0 37.0 38.0 55-59 37.217200000000005 38.0 38.0 38.0 37.0 38.0 60-64 37.1682 38.0 38.0 38.0 37.0 38.0 65-69 37.0987 38.0 38.0 38.0 37.0 38.0 70-74 37.1534 38.0 38.0 38.0 36.8 38.0 75-79 37.0963 38.0 38.0 38.0 36.6 38.0 80-84 36.9469 38.0 38.0 38.0 35.8 38.0 85-89 36.986900000000006 38.0 38.0 38.0 36.0 38.0 90-94 36.8941 38.0 38.0 38.0 36.0 38.0 95-99 36.82185 38.0 38.0 38.0 35.4 38.0 100-104 36.507600000000004 38.0 38.0 38.0 34.6 38.0 105-109 36.3838 38.0 38.0 38.0 34.2 38.0 110-114 35.96225 38.0 38.0 38.0 33.0 38.0 115-119 35.878699999999995 38.0 37.8 38.0 32.8 38.0 120-124 35.87755 38.0 37.2 38.0 32.6 38.0 125-129 35.88250000000001 38.0 37.6 38.0 33.0 38.0 130-134 35.87715 38.0 37.4 38.0 33.0 38.0 135-139 35.4605 38.0 36.2 38.0 31.0 38.0 140-144 34.89275 38.0 36.0 38.0 30.2 38.0 145-149 33.91330000000001 38.0 34.2 38.0 24.0 38.0 150-151 29.3605 35.5 27.0 38.0 2.0 38.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 2.0 4 3.0 5 0.0 6 0.0 7 1.0 8 1.0 9 0.0 10 2.0 11 1.0 12 1.0 13 2.0 14 2.0 15 2.0 16 1.0 17 4.0 18 1.0 19 4.0 20 2.0 21 5.0 22 7.0 23 5.0 24 8.0 25 7.0 26 14.0 27 13.0 28 18.0 29 28.0 30 39.0 31 47.0 32 77.0 33 75.0 34 132.0 35 216.0 36 483.0 37 2795.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 43.35 16.975 11.525 28.15 2 29.95 20.575 30.425 19.05 3 22.775000000000002 22.45 33.800000000000004 20.974999999999998 4 26.525 31.45 21.85 20.175 5 27.85 33.975 20.275000000000002 17.9 6 24.2 33.975 22.400000000000002 19.425 7 21.425 19.725 37.0 21.85 8 22.8 22.025 27.900000000000002 27.275 9 25.074999999999996 22.325 29.225 23.375 10-14 27.12 25.34 25.025 22.515 15-19 26.05 25.435000000000002 26.565 21.95 20-24 26.88 25.215 26.35 21.555 25-29 26.22786836050815 25.932779833950185 25.932779833950185 21.906571971591475 30-34 26.427642764276428 25.54755475547555 26.34263426342634 21.682168216821683 35-39 26.213932089813476 26.47397109566435 25.718857828674302 21.593238985847876 40-44 26.200000000000003 25.72 25.685000000000002 22.395 45-49 26.5 26.26 25.224999999999998 22.015 50-54 27.534999999999997 25.380000000000003 25.52 21.565 55-59 26.755000000000003 25.6 25.655 21.990000000000002 60-64 26.7026702670267 24.88248824882488 26.44264426442644 21.972197219721973 65-69 26.915383076615324 25.69013802760552 25.580116023204642 21.814362872574513 70-74 27.275910364145656 25.08503401360544 25.880352140856345 21.75870348139256 75-79 27.16 24.845 25.55 22.445 80-84 27.105328996747563 24.408306229672256 26.77508131098324 21.71128346259695 85-89 26.625325065013 25.76015203040608 25.150030006001202 22.464492898579717 90-94 26.6 25.924999999999997 25.685000000000002 21.790000000000003 95-99 26.950000000000003 25.165 25.485000000000003 22.400000000000002 100-104 27.08531398548912 25.769326995246434 25.85939454590943 21.285964473355016 105-109 27.419758650042564 24.460467678133295 26.468379149767163 21.651394522056982 110-114 26.240000000000002 25.900000000000002 25.905 21.955 115-119 26.945000000000004 26.195 25.424999999999997 21.435000000000002 120-124 26.805 26.43 24.595 22.17 125-129 27.204080612091815 26.593989098364755 24.253638045706857 21.948292243836576 130-134 27.44234905707568 26.27182232004402 24.576059226651996 21.709769396228303 135-139 27.204080612091815 25.953893083962594 25.28379256888533 21.55823373506026 140-144 27.744710648727057 25.368879107687693 25.6139648877107 21.272445355874556 145-149 28.18049927460103 26.12436840262144 25.198859372654958 20.496272950122567 150-151 28.88610763454318 25.231539424280353 24.90613266583229 20.97622027534418 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2.0 1 1.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.5 17 0.5 18 0.5 19 0.5 20 0.0 21 0.5 22 1.5 23 3.0 24 6.5 25 10.0 26 10.5 27 10.0 28 13.0 29 17.0 30 18.0 31 28.0 32 32.0 33 24.5 34 38.5 35 52.5 36 57.5 37 86.0 38 112.0 39 127.0 40 151.5 41 150.0 42 136.0 43 154.0 44 170.0 45 138.0 46 123.0 47 115.5 48 89.0 49 92.0 50 107.5 51 108.0 52 90.5 53 128.5 54 202.5 55 224.0 56 210.5 57 164.5 58 134.5 59 142.0 60 116.0 61 84.0 62 70.5 63 53.5 64 32.0 65 23.0 66 17.0 67 19.0 68 23.5 69 17.0 70 11.0 71 7.0 72 7.0 73 5.5 74 7.5 75 7.0 76 6.5 77 5.0 78 2.0 79 1.5 80 0.5 81 0.5 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.03 30-34 0.01 35-39 0.015 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.01 65-69 0.02 70-74 0.04 75-79 0.0 80-84 0.075 85-89 0.02 90-94 0.0 95-99 0.0 100-104 0.075 105-109 0.145 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.015 130-134 0.045 135-139 0.015 140-144 0.034999999999999996 145-149 0.055 150-151 0.125 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 72.125 #Duplication Level Percentage of deduplicated Percentage of total 1 80.86655112651646 58.325 2 11.369150779896014 16.400000000000002 3 3.9168110918544192 8.475000000000001 4 1.4211438474870017 4.1000000000000005 5 0.8318890814558059 3.0 6 0.6239168110918544 2.7 7 0.17331022530329288 0.8750000000000001 8 0.17331022530329288 1.0 9 0.34662045060658575 2.25 >10 0.2772963604852686 2.875 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTTCGATCCTGGCTCAGGATGAACGCTGGCGGCATGCTTAACACATGCAA 27 0.675 No Hit CCTTACCAGGGCTTGACATGCCGCGAATCCTCTTGAAAGAGAGGGGTGCC 20 0.5 No Hit CCTGGCTCAGGATGAACGCTGGCGGCATGCTTAACACATGCAAGTCGAAC 15 0.375 No Hit ATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAA 13 0.325 No Hit GTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGATCTT 10 0.25 No Hit GTGACCAGGCCCGGGCGACTGTTTACCAAAAACACAGGTCTCCGCAAAGT 10 0.25 No Hit GGCTTGCGGTGGATACCTAGGCACCCAGAGACGAGGAAGGGCGTAGCAAG 10 0.25 No Hit TATCTATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTT 10 0.25 No Hit TGGGAACTTAGTTTCCGTTTGGGTATGCGCCCTTGGATTGCTGTTGCATA 9 0.22499999999999998 No Hit GCTTGTTATATGGGTCGTGAGTGGGAACTTAGTTTCCGTTTGGGTATGCG 9 0.22499999999999998 No Hit CTTACCAGGGCTTGACATGCCGCGAATCCTCTTGAAAGAGAGGGGTGCCC 9 0.22499999999999998 No Hit GACCAGGCCCGGGCGACTGTTTACCAAAAACACAGGTCTCCGCAAAGTCG 9 0.22499999999999998 No Hit GCTGCGACTGCTGTTTTCTTGATTTACCCTATTGGTCAAGGAAGCTTCTC 9 0.22499999999999998 No Hit GTCAAGGTCGCGTTATTAATACTTGGGCTGATATCATCAACCGTGCTAAT 9 0.22499999999999998 No Hit CCTGAACAGACCGCCGGTGTTAAGCCGGAGGAAGGAGAGGATGAGGCCAA 9 0.22499999999999998 No Hit GCACAACATCCTTATGCATCCATTTCACATGTTAGGTGTAGCTGGTGTTT 9 0.22499999999999998 No Hit GTTCAACCTAAATGGATTCAATTTCAACCAATCTGTAGTTGATAGTCAAG 9 0.22499999999999998 No Hit GCTAACTCCAAAAACCCGTCCTCAGTTCGGATTGCAGGCTGCAACTCGCC 9 0.22499999999999998 No Hit CTTGCGGTGGATACCTAGGCACCCAGAGACGAGGAAGGGCGTAGCAAGCG 8 0.2 No Hit TTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAG 8 0.2 No Hit ATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCA 8 0.2 No Hit CAGTGACCAGGCCCGGGCGACTGTTTACCAAAAACACAGGTCTCCGCAAA 8 0.2 No Hit GGATGATCAGCCACACTGGGACTGAGACACGGCCCAGACTCCTACGGGAG 8 0.2 No Hit GTTAAGTCTCGCAACGAGCGCAACCCTCGTGTTTAGTTGCCACTATGAGT 7 0.17500000000000002 No Hit TATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAA 7 0.17500000000000002 No Hit GCTTGCGGTGGATACCTAGGCACCCAGAGACGAGGAAGGGCGTAGCAAGC 7 0.17500000000000002 No Hit CCCTATTCAGTGCTATGCATGGTTCCTTGGTAACCTCTAGTTTGATCAGG 7 0.17500000000000002 No Hit CGCGAAAGTACAAGCCTGTGGGGTCGCTTCTGCAACTGGATAACTAGCAC 7 0.17500000000000002 No Hit GGTCGCTTCTGCAACTGGATAACTAGCACTGAAAATCGTCTTTACATCGG 6 0.15 No Hit GGCTGATCTTCCCCAAGAGTCCACATCGACGGGAAGGTTTGGCACCTCGA 6 0.15 No Hit GAACCTTACCAGGGCTTGACATGCCGCGAATCCTCTTGAAAGAGAGGGGT 6 0.15 No Hit CCTAAATGGATTCAATTTCAACCAATCTGTAGTTGATAGTCAAGGTCGCG 6 0.15 No Hit GGATGAACGCTGGCGGCATGCTTAACACATGCAAGTCGAACGGGAAGTGG 6 0.15 No Hit CCTAGCCGTAAACGATGGATACTAGGTGCTGTGCGACTCGACCCGTGCAG 6 0.15 No Hit GTTGAAAATAAGCATAGATCCGGAGATTCCCAAATAGGTCAACCTTTTAA 6 0.15 No Hit GAACGCTGGCGGCATGCTTAACACATGCAAGTCGAACGGGAAGTGGTGTT 6 0.15 No Hit GCTAGTTTCAACAACTCTCGTTCTTTACACTTCTTCTTGGCTGCTTGGCC 6 0.15 No Hit GGAACAACAACTGGAAACGGTTGCTAATACCCCGTAGGCTGAGGAGCAAA 6 0.15 No Hit GTTATTTTGGCCGATTAATCTTCCAATATGCTAGTTTCAACAACTCTCGT 6 0.15 No Hit GGCGGGACTACCCGCTGAGTTTAAGCATATAAATAAGCGGAGGAGAAGAA 6 0.15 No Hit CCTATTCAGTGCTATGCATGGTTCCTTGGTAACCTCTAGTTTGATCAGGG 6 0.15 No Hit CATCAACCGTGCTAATCTTGGTATGGAAGTAATGCACGAACGTAATGCTC 6 0.15 No Hit CACACGTGCTACAATGGGCGGGACAAAGGGTCGCGATCTCGCGAGGGTGA 6 0.15 No Hit GGTGACCCTGCTTTTTCAGGGTAAGAAGGGGTAGAGAAAATGCCTCGAGC 6 0.15 No Hit GTTGGGTTAAGTCTCGCAACGAGCGCAACCCTCGTGTTTAGTTGCCACTA 6 0.15 No Hit GTTAGGTGTAGCTGGTGTTTTCGGCGGTTCCCTATTCAGTGCTATGCATG 6 0.15 No Hit AGCACAACATCCTTATGCATCCATTTCACATGTTAGGTGTAGCTGGTGTT 5 0.125 No Hit GTTGCTAATACCCCGTAGGCTGAGGAGCAAAAGGAGAAATCCGCCCAAGG 5 0.125 No Hit CTGGTACTTTCAACTTTATGATTGTATTCCAGGCAGAGCACAACATCCTT 5 0.125 No Hit GGGACAGTCTCAGGTAGACAGTTTCTATGGGGCGTAGGCCTCCCAAAAGG 5 0.125 No Hit GGGAAACTACTGAAAATGAATCTGCTAATGAGGGTTACAAATTTGGTCAA 5 0.125 No Hit CGTTGATGAATGGTTATACAATGGTGGTCCTTATGAGCTAATTGTTCTAC 5 0.125 No Hit GTTCTGGGCCGCACGCGCGCTACACTGATGTATTCAACGAGTATATAGCC 5 0.125 No Hit GATGAACGCTGGCGGCATGCTTAACACATGCAAGTCGAACGGGAAGTGGT 5 0.125 No Hit CCTGCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGCAA 5 0.125 No Hit CTTCTTGGCTGCTTGGCCTGTAGTAGGAATCTGGTTCACTGCTTTAGGTA 5 0.125 No Hit GTTTTGATGATCCCTACCTTATTGACCGCAACTTCTGTATTTATTATCGC 5 0.125 No Hit ATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATA 5 0.125 No Hit CTGACACTGAGAGACGAAAGCTAGGGGAGCAAATGGGATTAGAGACCCCA 5 0.125 No Hit AGTCAAGGTCGCGTTATTAATACTTGGGCTGATATCATCAACCGTGCTAA 5 0.125 No Hit GTGGGAACTTAGTTTCCGTTTGGGTATGCGCCCTTGGATTGCTGTTGCAT 5 0.125 No Hit GGGAAGTGGTGTTTCCAGTGGCGAACGGGTGAGTAACGCGTAAGAACCTG 5 0.125 No Hit ATCCTGGCTCAGGATGAACGCTGGCGGCATGCTTAACACATGCAAGTCGA 5 0.125 No Hit TCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGA 5 0.125 No Hit GTGTTTTCGGCGGTTCCCTATTCAGTGCTATGCATGGTTCCTTGGTAACC 5 0.125 No Hit GGGGAACAACAACTGGAAACGGTTGCTAATACCCCGTAGGCTGAGGAGCA 5 0.125 No Hit CTTCCAATATGCTAGTTTCAACAACTCTCGTTCTTTACACTTCTTCTTGG 5 0.125 No Hit ATCTATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTG 5 0.125 No Hit GCTTAACACATGCAAGTCGAACGGGAAGTGGTGTTTCCAGTGGCGAACGG 5 0.125 No Hit GTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGATCTTACCATGACTG 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.025 0.0 0.0 0.0 0.0 66-67 0.05 0.0 0.0 0.0 0.0 68-69 0.05 0.0 0.0 0.0 0.0 70-71 0.05 0.0 0.0 0.0 0.0 72-73 0.0625 0.0 0.0 0.0 0.0 74-75 0.075 0.0 0.0 0.0 0.0 76-77 0.075 0.0 0.0 0.0 0.0 78-79 0.1125 0.0 0.0 0.0 0.0 80-81 0.1375 0.0 0.0 0.0 0.0 82-83 0.175 0.0 0.0 0.0 0.0 84-85 0.2375 0.0 0.0 0.0 0.0 86-87 0.2875 0.0 0.0 0.0 0.0 88-89 0.32499999999999996 0.0 0.0 0.0 0.0 90-91 0.4375 0.0 0.0 0.0 0.0 92-93 0.6625000000000001 0.0 0.0 0.0 0.0 94-95 0.75 0.0 0.0 0.0 0.0 96-97 0.9375 0.0 0.0 0.0 0.0 98-99 1.15 0.0 0.0 0.0 0.0 100-101 1.35 0.0 0.0 0.0 0.0 102-103 1.625 0.0 0.0 0.0 0.0 104-105 1.925 0.0 0.0 0.0 0.0 106-107 2.1875 0.0 0.0 0.0 0.0 108-109 2.7 0.0 0.0 0.0 0.0 110-111 3.2 0.0 0.0 0.0 0.0 112-113 3.8 0.0 0.0 0.0 0.0 114-115 4.262499999999999 0.0 0.0 0.0 0.0 116-117 4.775 0.0 0.0 0.0 0.0 118-119 5.362500000000001 0.0 0.0 0.0 0.0 120-121 6.0375 0.0 0.0 0.0 0.0 122-123 6.7875 0.0 0.0 0.0 0.0 124-125 7.5375 0.0 0.0 0.0 0.0 126-127 8.25 0.0 0.0 0.0 0.0 128-129 9.075 0.0 0.0 0.0 0.0 130-131 9.662500000000001 0.0 0.0 0.0 0.0 132-133 10.2375 0.0 0.0 0.0 0.0 134-135 11.0625 0.0 0.0 0.0 0.0 136-137 11.85 0.0 0.0 0.0 0.0 138-139 12.375 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCGGAGG 10 0.006830828 145.0 1 CCCGGCC 10 0.006830828 145.0 6 GAGTAAC 10 0.006830828 145.0 9 AGGAGAG 10 0.006830828 145.0 9 GGGGGGG 20 0.00593511 29.0 80-84 >>END_MODULE Read 1029034 spots for SRR6941599.sra Written 1029034 spots for SRR6941599.sra Read 1029034 spots for SRR6941599.sra Written 1029034 spots for SRR6941599.sra Read 1029034 spots for SRR6941599.sra Written 1029034 spots for SRR6941599.sra Read 1029034 spots for SRR6941599.sra Written 1029034 spots for SRR6941599.sra Read 1029034 spots for SRR6941599.sra Written 1029034 spots for SRR6941599.sra Read 1029034 spots for SRR6941599.sra Written 1029034 spots for SRR6941599.sra Read 1029034 spots for SRR6941599.sra Written 1029034 spots for SRR6941599.sra Read 1029034 spots for SRR6941599.sra Written 1029034 spots for SRR6941599.sra Read 1029034 spots for SRR6941599.sra Written 1029034 spots for SRR6941599.sra Read 1029034 spots for SRR6941599.sra Written 1029034 spots for SRR6941599.sra Read 1029034 spots for SRR6941599.sra Written 1029034 spots for SRR6941599.sra Read 1029034 spots for SRR6941599.sra Written 1029034 spots for SRR6941599.sra Read 1029034 spots for SRR6941599.sra Written 1029034 spots for SRR6941599.sra Read 1029038 spots for SRR6941599.sra Written 1029038 spots for SRR6941599.sra Read 1029034 spots for SRR6941599.sra Written 1029034 spots for SRR6941599.sra Read 1029034 spots for SRR6941599.sra Written 1029034 spots for SRR6941599.sra Read 1029034 spots for SRR6941599.sra Written 1029034 spots for SRR6941599.sra Read 1029034 spots for SRR6941599.sra Written 1029034 spots for SRR6941599.sra Read 1029034 spots for SRR6941599.sra Written 1029034 spots for SRR6941599.sra Read 1029034 spots for SRR6941599.sra Written 1029034 spots for SRR6941599.sra SRR ids: ['SRR6941599.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_dbbao2le SRR6941599.sra spots: 20580684 blocks: [[1, 1029034], [1029035, 2058068], [2058069, 3087102], [3087103, 4116136], [4116137, 5145170], [5145171, 6174204], [6174205, 7203238], [7203239, 8232272], [8232273, 9261306], [9261307, 10290340], [10290341, 11319374], [11319375, 12348408], [12348409, 13377442], [13377443, 14406476], [14406477, 15435510], [15435511, 16464544], [16464545, 17493578], [17493579, 18522612], [18522613, 19551646], [19551647, 20580684]] SRR6941599 file size 6952418 SRR6941599 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR6941599 SRR6941599_1.fastq SRR6941599_2.fastq Input file: SRR6941599_1.fastq Paired file: SRR6941599_2.fastq trimmed: SRR6941599-trimmed-pair1.fastq, SRR6941599-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Fri Dec 6 12:44:00 2024 >> started Fri Dec 6 12:44:24 2024 >> done (24.109s) 20580684 read pairs processed; of these: 9275 ( 0.05%) short read pairs filtered out after trimming by size control 6491 ( 0.03%) empty read pairs filtered out after trimming by size control 20564918 (99.92%) read pairs available; of these: 10362070 (50.39%) trimmed read pairs available after processing 10202848 (49.61%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 19 7 0.00% 20 1 0.00% 21 5 0.00% 22 0 0.00% 23 3 0.00% 24 2 0.00% 25 7 0.00% 26 6 0.00% 27 26 0.00% 28 7 0.00% 29 3 0.00% 30 6 0.00% 31 10 0.00% 32 9 0.00% 33 8 0.00% 34 10 0.00% 35 13 0.00% 36 16 0.00% 37 12 0.00% 38 11 0.00% 39 16 0.00% 40 24 0.00% 41 19 0.00% 42 28 0.00% 43 27 0.00% 44 19 0.00% 45 28 0.00% 46 28 0.00% 47 48 0.00% 48 50 0.00% 49 63 0.00% 50 69 0.00% 51 92 0.00% 52 121 0.00% 53 111 0.00% 54 125 0.00% 55 164 0.00% 56 166 0.00% 57 165 0.00% 58 207 0.00% 59 306 0.00% 60 327 0.00% 61 403 0.00% 62 477 0.00% 63 546 0.00% 64 641 0.00% 65 707 0.00% 66 769 0.00% 67 913 0.00% 68 1015 0.00% 69 1137 0.01% 70 1298 0.01% 71 1604 0.01% 72 1912 0.01% 73 2138 0.01% 74 2356 0.01% 75 2801 0.01% 76 3058 0.01% 77 3514 0.02% 78 3901 0.02% 79 4693 0.02% 80 5503 0.03% 81 5862 0.03% 82 6730 0.03% 83 7755 0.04% 84 8588 0.04% 85 10072 0.05% 86 10661 0.05% 87 11794 0.06% 88 13802 0.07% 89 14219 0.07% 90 15695 0.08% 91 16599 0.08% 92 18602 0.09% 93 19799 0.10% 94 21659 0.11% 95 24201 0.12% 96 24077 0.12% 97 27109 0.13% 98 27751 0.13% 99 30028 0.15% 100 30615 0.15% 101 33479 0.16% 102 34801 0.17% 103 35430 0.17% 104 38721 0.19% 105 39673 0.19% 106 41423 0.20% 107 43417 0.21% 108 46122 0.22% 109 48217 0.23% 110 48286 0.23% 111 50042 0.24% 112 51515 0.25% 113 52790 0.26% 114 56666 0.28% 115 60484 0.29% 116 61279 0.30% 117 61640 0.30% 118 62591 0.30% 119 62446 0.30% 120 66830 0.32% 121 69915 0.34% 122 73367 0.36% 123 76423 0.37% 124 76690 0.37% 125 82472 0.40% 126 80281 0.39% 127 82060 0.40% 128 82095 0.40% 129 86050 0.42% 130 83130 0.40% 131 87935 0.43% 132 91804 0.45% 133 90448 0.44% 134 96285 0.47% 135 97300 0.47% 136 101788 0.49% 137 102378 0.50% 138 109727 0.53% 139 115463 0.56% 140 118502 0.58% 141 133626 0.65% 142 136209 0.66% 143 150921 0.73% 144 167884 0.82% 145 199190 0.97% 146 230320 1.12% 147 294726 1.43% 148 425616 2.07% 149 812486 3.95% 150 4623758 22.48% 151 10202848 49.61% 20564918 reads passed initial QC criterion=sequence-density sequence-density=0.99 sequence-density-rank=1 fanout-score=3.22 fanout-score-rank=23 prefix-density=1.66 prefix-fanout=1.9 sequence=ATTTAGCCTTGCAAGGTGGTCCTTGCTGATTCACACGGGATTCCACGTGCCCCATGCTACTCGGGTCAGAGCGTAAGCTAGTGATGCTTTCGGCTACTGGACTTTAGCCATCTAGGGTGCGGCACTCAACCGCTTCGCCTAGCAGCACAACGCTTGTATTGCTCTCCCACAACCCCGTTTTCACGGTTTAGGCTGCTCCCATTTCGCTCGCCGCTACTACGGGAATCGCTTTTGCTTTCTTTTCCTCTGGCTACTAAGATGTTTCAGTTCGCCAGGTTGTCTCTTGCCTGCTCATGGATTCAGCAGGCAGTTTAAAAGGTTGACCTATTTGGGAATCTCCGGATCTATGCTTATTTTCAACTCCCCGAAGCATTTCGTCGCTTGCTACGCCCTTCCTCGTCTCTGGGTGCCTAGGTATCCACCGCAAGCCTTTCCTCTTTTGAACCTCGCCATTAAC criterion=fanout-score sequence-density=0.01 sequence-density-rank=41 fanout-score=81.76 fanout-score-rank=1 prefix-density=0.12 prefix-fanout=6.1 sequence=AAAAAAAAGGGGGGGTAAGGACCCGCTAAGCTCCTACTTTTTCATGTTTCCAATCCGATCCCTCCGATTACTATAGAGATGAACCCAATCCAGAATATGAACCATAAAAGAAAACACCTACTAAACCAATCACAAGAATACCAGTTACCGTACCTATCAGCCAAAGAGGAATTCTTCCAGTAGTATCGGCCATTTCCCCTACTTTCCTCCACATTTTATCAAGTGGTCATGCTAGAGACAAAAACAGTCATGGATAGTTATGTTATAAGGATGGTATCCTTCCAAATGGGATAAGAGAGTTCTTACTACTCTCTTCTTTTCTCTCAATTAAAGAAGTAATTGGAAAACAAAACAGCAAGTACAAAAATGAGTAATAAACCCCAGTATAGACTGGTACGATTCAATTCAACATTTTGTTCATTCGGGTTTGATTGTGTCATAGTTCTATAGTTGGAATTTAGTTTATCGTTGGATGAACTGCATTGCTGATATTGATCCCAAGAAAAAAACA criterion=sequence-density sequence-density=0.48 sequence-density-rank=1 fanout-score=8.62 fanout-score-rank=5 prefix-density=3.03 prefix-fanout=1.4 sequence=TGGTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGTTAACGAACGAGACCTCAGCCTGCTAACTAGCTATGCGGAGCCATCCCTCCGCAGCTAGCTTCTTAGAGGGACTATCGCCGTTTAGGCGACGGAAGTTTGAGGCAATAACAGGTCTGTGATGCCCTTAGATGTTCTGGGCCGCACGCGCGCTACACTGATGTATTCAACGAGTATATAGCCTTGGCCGACAGGCCCGGGTAATCTTGGGAAATTTCATCGTGATGGGGATAGATCATTGCAATTGTTGGTCTTCAACGAGGAATGCCTAGTAAGCGCGAGTCATCAGCTCGCGTTGACTACGTCCCTGCCCTTTGTACACACC criterion=fanout-score sequence-density=0.01 sequence-density-rank=36 fanout-score=74.06 fanout-score-rank=1 prefix-density=0.77 prefix-fanout=1.2 sequence=AGAAGGGGTGCCCCCTCACAAAAGGGGGTCGCAGTGACCAGGCCCGGGCGACTGTTTACCAAAAACACAGGTCTCCGCAAAGTCGTAAGACCATGTATGGGGGCTGACGCCTGCCCAGTGCCGGAAGGTCAAGGAAGTTGGTGAACTGATGACAGGGAAGCCGGCGACCGAAGCCCCGGTGAACGGCGGCCGTAAC SRR6941599 testing PE reads STAR mapping to Ensembl genome Started job on | Dec 06 12:45:45 Started mapping on | Dec 06 12:45:46 Finished on | Dec 06 12:47:33 Mapping speed, Million of reads per hour | 691.90 Number of input reads | 20564918 Average input read length | 291 UNIQUE READS: Uniquely mapped reads number | 10036578 Uniquely mapped reads % | 48.80% Average mapped length | 293.67 Number of splices: Total | 1738714 Number of splices: Annotated (sjdb) | 1576054 Number of splices: GT/AG | 1667513 Number of splices: GC/AG | 20236 Number of splices: AT/AC | 5081 Number of splices: Non-canonical | 45884 Mismatch rate per base, % | 0.16% Deletion rate per base | 0.01% Deletion average length | 1.63 Insertion rate per base | 0.01% Insertion average length | 1.72 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 7983369 % of reads mapped to multiple loci | 38.82% Number of reads mapped to too many loci | 309495 % of reads mapped to too many loci | 1.50% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 3.22% % of reads unmapped: other | 7.65% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 2550238 2550238 2550238 N_multimapping 7983369 7983369 7983369 N_noFeature 5246418 9759599 5353945 N_ambiguous 340177 5408 175817 UnstrandedReadsAssigned:4449983 PositiveStrandReadsAssigned:271571 NegativeStrandReadsAssigned:4506816 Dataset is classified negative stranded MeadianReadLen=151 20thPercentileLength=148 echo kmer=143 SRR6941599 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,972 [index] number of k-mers: 66,720,672 [index] number of equivalence classes: 111,837 [quant] running in paired-end mode [quant] will process pair 1: SRR6941599-trimmed-pair1.fastq SRR6941599-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 20,564,918 reads, 8,539,470 reads pseudoaligned [quant] estimated average fragment length: 210.869 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,023 rounds 52973 SRR6941599.ke.tsv 35125 SRR6941599.se.tsv 88098 total ==> SRR6941599.ke.tsv <== target_id length eff_length est_counts tpm PNS24245 936 726.565 0 0 PNS24247 1044 834.131 2.28736 0.213686 PNS24249 1928 1718.13 4.9186 0.22308 PNS24246 1044 834.131 2.28736 0.213686 PNS24248 1044 834.131 2.28736 0.213686 PNS24244 1471 1261.13 5.21934 0.322502 PNS24243 293 113.873 0 0 KQK14069 1603 1393.13 924.661 51.721 KQK14071 474 272.896 34.5239 9.85825 ==> SRR6941599.se.tsv <== BRADI_1g14170v3 1142 BRADI_1g53295v3 11 BRADI_1g59795v3 8 BRADI_1g07683v3 0 BRADI_1g00485v3 1 BRADI_1g20270v3 48 BRADI_1g74790v3 11 BRADI_1g09890v3 1 BRADI_1g77505v3 32 BRADI_1g48960v3 0 SRR6941599 completed mapping pipeline successfully