Starting /dee2/code/volunteer_pipeline.sh SRR6941600 current disk space = 1551361445888 free memory = 1599164480 SRR6941600 SRAfilesize e05c3773b348afe890d6e315cb6019b6 SRR6941600.sra SRR6941600.sra file validated SRR6941600 is paired end SRR6941600 is conventional basespace SRR6941600 read1 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR6941600_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 26.65825 31.0 25.0 32.0 18.0 33.0 2 31.71525 33.0 31.0 33.0 28.0 33.0 3 32.15675 33.0 33.0 33.0 29.0 34.0 4 32.651 33.0 33.0 34.0 31.0 34.0 5 33.18175 34.0 33.0 34.0 33.0 34.0 6 37.0025 38.0 37.0 38.0 35.0 38.0 7 37.2975 38.0 38.0 38.0 36.0 38.0 8 37.486 38.0 38.0 38.0 37.0 38.0 9 37.5865 38.0 38.0 38.0 38.0 38.0 10-14 37.503600000000006 38.0 38.0 38.0 37.6 38.0 15-19 37.33885 38.0 38.0 38.0 37.2 38.0 20-24 37.4822 38.0 38.0 38.0 37.4 38.0 25-29 37.58445 38.0 38.0 38.0 37.8 38.0 30-34 37.61515000000001 38.0 38.0 38.0 38.0 38.0 35-39 37.5378 38.0 38.0 38.0 38.0 38.0 40-44 37.50055 38.0 38.0 38.0 37.6 38.0 45-49 37.4888 38.0 38.0 38.0 37.4 38.0 50-54 37.34439999999999 38.0 38.0 38.0 37.0 38.0 55-59 37.1767 38.0 38.0 38.0 36.2 38.0 60-64 37.31525 38.0 38.0 38.0 37.0 38.0 65-69 37.214749999999995 38.0 38.0 38.0 36.6 38.0 70-74 37.25265 38.0 38.0 38.0 36.6 38.0 75-79 37.22615 38.0 38.0 38.0 36.8 38.0 80-84 37.254450000000006 38.0 38.0 38.0 36.8 38.0 85-89 37.169349999999994 38.0 38.0 38.0 36.4 38.0 90-94 36.21325 38.0 37.2 38.0 30.6 38.0 95-99 36.7567 38.0 37.8 38.0 35.2 38.0 100-104 37.09310000000001 38.0 38.0 38.0 36.0 38.0 105-109 36.9305 38.0 38.0 38.0 35.8 38.0 110-114 36.88645 38.0 38.0 38.0 35.4 38.0 115-119 36.6956 38.0 38.0 38.0 34.8 38.0 120-124 36.57165 38.0 38.0 38.0 34.6 38.0 125-129 36.5037 38.0 38.0 38.0 34.0 38.0 130-134 36.5302 38.0 38.0 38.0 34.2 38.0 135-139 36.16195 38.0 38.0 38.0 33.4 38.0 140-144 36.0218 38.0 37.8 38.0 33.0 38.0 145-149 35.55200000000001 38.0 36.6 38.0 32.2 38.0 150-151 32.89275 37.0 33.5 38.0 16.5 38.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 11 1.0 12 1.0 13 0.0 14 2.0 15 2.0 16 1.0 17 0.0 18 2.0 19 1.0 20 2.0 21 2.0 22 1.0 23 3.0 24 2.0 25 5.0 26 11.0 27 5.0 28 14.0 29 25.0 30 29.0 31 45.0 32 62.0 33 70.0 34 120.0 35 195.0 36 493.0 37 2906.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 38.71316572046499 13.138686131386862 10.516355771830224 37.631792376317925 2 24.05 18.075 32.175 25.7 3 19.025 26.974999999999998 25.650000000000002 28.349999999999998 4 22.650000000000002 32.45 23.525 21.375 5 20.95 36.225 24.474999999999998 18.35 6 18.525 38.0 24.075 19.400000000000002 7 14.025000000000002 25.525 42.199999999999996 18.25 8 18.575 25.124999999999996 29.025000000000002 27.275 9 17.474999999999998 24.2 30.75 27.575 10-14 20.669999999999998 32.295 23.355 23.68 15-19 20.65 28.575 26.775 24.0 20-24 20.46 29.93 26.195 23.415 25-29 22.375 28.999999999999996 25.595000000000002 23.03 30-34 21.215 30.919999999999998 25.0 22.865 35-39 20.995 30.035 25.580000000000002 23.39 40-44 20.215 28.645 27.095000000000002 24.044999999999998 45-49 20.19 30.314999999999998 26.915 22.58 50-54 21.11 29.659999999999997 25.75 23.48 55-59 20.955 29.635 25.635 23.775 60-64 20.125 29.285 26.790000000000003 23.799999999999997 65-69 21.5 29.48 24.605 24.415 70-74 21.555 29.26 25.4 23.785 75-79 21.33 29.17 25.679999999999996 23.82 80-84 22.27 28.93 25.3 23.5 85-89 21.099999999999998 28.58 26.44 23.880000000000003 90-94 21.46 29.585 24.84 24.115000000000002 95-99 20.495 29.375 25.8 24.33 100-104 20.765 29.195 25.775 24.265 105-109 21.279999999999998 28.28 26.305 24.135 110-114 20.61 28.775000000000002 26.200000000000003 24.415 115-119 20.435 29.585 25.130000000000003 24.85 120-124 20.1 30.080000000000002 23.385 26.435 125-129 21.41 29.759999999999998 25.295 23.535 130-134 22.165000000000003 30.005 23.915 23.915 135-139 23.155 29.044999999999998 24.695 23.105 140-144 22.395 29.93 24.625 23.05 145-149 22.31 28.9 24.154999999999998 24.635 150-151 20.3375 29.562500000000004 23.849999999999998 26.25 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.5 10 0.5 11 0.0 12 0.0 13 0.0 14 0.5 15 1.0 16 0.5 17 0.0 18 0.0 19 0.0 20 0.5 21 3.0 22 6.5 23 5.5 24 3.0 25 4.5 26 9.5 27 14.0 28 14.5 29 14.0 30 24.5 31 33.5 32 33.0 33 37.5 34 38.5 35 44.0 36 101.0 37 225.0 38 232.5 39 180.0 40 229.0 41 260.0 42 231.5 43 215.5 44 212.5 45 197.5 46 159.0 47 114.0 48 105.5 49 104.0 50 91.5 51 82.0 52 75.5 53 80.5 54 92.5 55 109.0 56 120.0 57 91.0 58 79.0 59 76.0 60 55.0 61 40.5 62 29.5 63 24.5 64 23.0 65 21.0 66 13.5 67 9.0 68 7.5 69 4.0 70 4.5 71 4.5 72 3.0 73 2.5 74 1.0 75 1.5 76 2.0 77 0.5 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content warn #Base N-Count 1 7.5249999999999995 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 71.35000000000001 #Duplication Level Percentage of deduplicated Percentage of total 1 84.37281009110022 60.199999999999996 2 8.33917309039944 11.899999999999999 3 2.943237561317449 6.3 4 1.5066573230553608 4.3 5 0.8409250175192713 3.0 6 0.5606166783461808 2.4 7 0.2803083391730904 1.4000000000000001 8 0.31534688156972673 1.7999999999999998 9 0.17519271198318148 1.125 >10 0.6306937631394535 5.875 >50 0.0350385423966363 1.7000000000000002 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCATTAAT 68 1.7000000000000002 No Hit TTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCATTAATA 23 0.575 No Hit GGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTACGTT 20 0.5 No Hit GGAAAAACCTTATCCATTAATAGCGGGAACTTCAAGAGCAGCTAGATCTA 17 0.42500000000000004 No Hit CTCATAAGGACCACCATTGTATAACCATTCATCAACGGATGCAGCTTCCC 15 0.375 No Hit GCCAAAATAACCATGAGCAGCCACAATATTATAAGTCTCTTCCTCTTGAC 13 0.325 No Hit CTCATTAGCAGATTCATTTTCAGTAGTTTCCCTGATCAAACTAGAGGTTA 13 0.325 No Hit GTTGAAACTAGCATATTGGAAGATTAATCGGCCAAAATAACCATGAGCAG 13 0.325 No Hit CTCGCGAATACCATCAATATCTACTGGAGGGGCTGCGATGAAGGCGATAA 13 0.325 No Hit GTGAAATGGATGCATAAGGATGTTGTGCTCTGCCTGGAATACAATCATAA 12 0.3 No Hit CTTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCAT 12 0.3 No Hit GGCCAAAATAACCATGAGCAGCCACAATATTATAAGTCTCTTCCTCTTGA 11 0.27499999999999997 No Hit GCCGAAAACACCAGCTACACCTAACATGTGAAATGGATGCATAAGGATGT 11 0.27499999999999997 No Hit GGTCAATAAGGTAGGGATCATCAAAACACCGAACCATCCGATGTAAAGAC 11 0.27499999999999997 No Hit GGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTACGTTC 11 0.27499999999999997 No Hit CGCAGAAGTAGGAATAATAGCACCAGAGATAATATTGTTTCCATAAAGTA 10 0.25 No Hit GTACAAGGCCCGGGAACGGATTCACCGCCGTATGGCTGACCGGCGATTAC 10 0.25 No Hit GCATAAGGATGTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAAAGTA 10 0.25 No Hit ATAACAAGCTACACCAAGTAAGAAGTGTAGAACAATTAGCTCATAAGGAC 10 0.25 No Hit CGGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTACGT 9 0.22499999999999998 No Hit CAAGTATTAATAACGCGACCTTGACTATCAACTACAGATTGGTTGAAATT 9 0.22499999999999998 No Hit CTAACATGTGAAATGGATGCATAAGGATGTTGTGCTCTGCCTGGAATACA 9 0.22499999999999998 No Hit GCGGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTACG 9 0.22499999999999998 No Hit CTCTTGACCAAATTTGTAACCCTCATTAGCAGATTCATTTTCAGTAGTTT 9 0.22499999999999998 No Hit GTATTAATAACGCGACCTTGACTATCAACTACAGATTGGTTGAAATTGAA 8 0.2 No Hit GCTGAATATGCAACAGCAATCCAAGGGCGCATACCCAAACGGAAACTAAG 8 0.2 No Hit TTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCATT 8 0.2 No Hit GTGAGCATTACGTTCGTGCATTACTTCCATACCAAGATTAGCACGGTTGA 8 0.2 No Hit GCATATTGGAAGATTAATCGGCCAAAATAACCATGAGCAGCCACAATATT 8 0.2 No Hit CATATAACAAGCTACACCAAGTAAGAAGTGTAGAACAATTAGCTCATAAG 8 0.2 No Hit GTGCTCTGCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTCCTA 8 0.2 No Hit GAAAAACCTTATCCATTAATAGCGGGAACTTCAAGAGCAGCTAGATCTAG 8 0.2 No Hit GCGGAAAAACCTTATCCATTAATAGCGGGAACTTCAAGAGCAGCTAGATC 8 0.2 No Hit GGCGCATACCCAAACGGAAACTAAGTTCCCACTCACGACCCATATAACAA 7 0.17500000000000002 No Hit GCATTACGTTCGTGCATTACTTCCATACCAAGATTAGCACGGTTGATGAT 7 0.17500000000000002 No Hit TTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCATTA 7 0.17500000000000002 No Hit GCACTGAATAGGGAACCGCCGAAAACACCAGCTACACCTAACATGTGAAA 7 0.17500000000000002 No Hit CAAGGAACCATGCATAGCACTGAATAGGGAACCGCCGAAAACACCAGCTA 7 0.17500000000000002 No Hit GCTTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCA 7 0.17500000000000002 No Hit GGGAATTCGTAGATCCTCCAGACGTAGAGCACGTAGGGCTTTGAAACCAA 7 0.17500000000000002 No Hit GGTAAATCAAGAAAACAGCAGTCGCAGCTGCAACAGGAGCTGAATATGCA 7 0.17500000000000002 No Hit CAATAAGGTAGGGATCATCAAAACACCGAACCATCCGATGTAAAGACGAT 6 0.15 No Hit AAAAATTCTTATATGTTAGCGGAAAAACCTTATCCATTAATAGCGGGAAC 6 0.15 No Hit GGAGGGGCTGCGATGAAGGCGATAATAAATACAGAAGTTGCGGTCAATAA 6 0.15 No Hit GGAAGATTAATCGGCCAAAATAACCATGAGCAGCCACAATATTATAAGTC 6 0.15 No Hit CAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCATTAATAGCGGGAA 6 0.15 No Hit CCAAGAAGAAGTGTAAAGAACGAGAGTTGTTGAAACTAGCATATTGGAAG 6 0.15 No Hit TCTTGACCAAATTTGTAACCCTCATTAGCAGATTCATTTTCAGTAGTTTC 6 0.15 No Hit CCAGATTCCTACTACAGGCCAAGCAGCCAAGAAGAAGTGTAAAGAACGAG 6 0.15 No Hit GGATCATCAAAACACCGAACCATCCGATGTAAAGACGATTTTCAGTGCTA 6 0.15 No Hit GGGATCATCAAAACACCGAACCATCCGATGTAAAGACGATTTTCAGTGCT 6 0.15 No Hit GCTAGTTATCCAGTTGCAGAAGCGACCCCACAGGCTTGTACTTTCGCGTC 6 0.15 No Hit CGGAAAAACCTTATCCATTAATAGCGGGAACTTCAAGAGCAGCTAGATCT 6 0.15 No Hit CCATTGTATAACCATTCATCAACGGATGCAGCTTCCCAAATTGGGTAAAA 6 0.15 No Hit AGCGGAAAAACCTTATCCATTAATAGCGGGAACTTCAAGAGCAGCTAGAT 6 0.15 No Hit GAACAATTAGCTCATAAGGACCACCATTGTATAACCATTCATCAACGGAT 6 0.15 No Hit GCTAGATCTAGAGGGAAGTTGTGAGCATTACGTTCGTGCATTACTTCCAT 6 0.15 No Hit CGCGAATACCATCAATATCTACTGGAGGGGCTGCGATGAAGGCGATAATA 5 0.125 No Hit ACCAGCTACACCTAACATGTGAAATGGATGCATAAGGATGTTGTGCTCTG 5 0.125 No Hit AGAGAAGCTTCCTTGACCAATAGGGTAAATCAAGAAAACAGCAGTCGCAG 5 0.125 No Hit CTCCACCGCTTGTGCGGGCCCCCGTCAATTCCTTTGAGTTTCATTCTTGC 5 0.125 No Hit CCGCAGAAGTAGGAATAATAGCACCAGAGATAATATTGTTTCCATAAAGT 5 0.125 No Hit GGCATGATGACTTGGCCTCATCCTCTCCTTCCTCCGGCTTAACACCGGCG 5 0.125 No Hit CTACTACAGGCCAAGCAGCCAAGAAGAAGTGTAAAGAACGAGAGTTGTTG 5 0.125 No Hit GTCGCAGCTGCAACAGGAGCTGAATATGCAACAGCAATCCAAGGGCGCAT 5 0.125 No Hit ATAAGGACCACCATTGTATAACCATTCATCAACGGATGCAGCTTCCCAAA 5 0.125 No Hit GGGAAGTTGTGAGCATTACGTTCGTGCATTACTTCCATACCAAGATTAGC 5 0.125 No Hit GTGAATGTGAAGAAGTAAGCCATTGTCGCGGCAATAATGAGCCAAAGTAG 5 0.125 No Hit GGATGCATAAGGATGTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAA 5 0.125 No Hit CCCTCATTAGCAGATTCATTTTCAGTAGTTTCCCTGATCAAACTAGAGGT 5 0.125 No Hit GGCTGATCATCCTCTCGGACCAGCTACTGATCATCGCCTTGGTAAGCTAT 5 0.125 No Hit CATCAGAGAAGCTTCCTTGACCAATAGGGTAAATCAAGAAAACAGCAGTC 5 0.125 No Hit GTGGTTCGCTGGAGTAGGCTGCGAGGCCCAAGCGGCCAAGGAGTGCAGCG 5 0.125 No Hit CCAAGTATTAATAACGCGACCTTGACTATCAACTACAGATTGGTTGAAAT 5 0.125 No Hit CAAAGATTTCGGTCAGAGCTGGCATATGCCAAACATGAATACCACCTGAA 5 0.125 No Hit GTTAGCGGAAAAACCTTATCCATTAATAGCGGGAACTTCAAGAGCAGCTA 5 0.125 No Hit CAGAGATAATATTGTTTCCATAAAGTAAAGAACCAGAAACAGGCTCGCGA 5 0.125 No Hit GTGCTAGTTATCCAGTTGCAGAAGCGACCCCACAGGCTTGTACTTTCGCG 5 0.125 No Hit ATATAACAAGCTACACCAAGTAAGAAGTGTAGAACAATTAGCTCATAAGG 5 0.125 No Hit CATAAGGATGTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAAAGTAC 5 0.125 No Hit CAGGCTTGTACTTTCGCGTCTCTCTAAAATTGCAGTCATGGTAAGATCTT 5 0.125 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.0125 0.0 0.0 0.0 0.0 70-71 0.025 0.0 0.0 0.0 0.0 72-73 0.025 0.0 0.0 0.0 0.0 74-75 0.025 0.0 0.0 0.0 0.0 76-77 0.025 0.0 0.0 0.0 0.0 78-79 0.025 0.0 0.0 0.0 0.0 80-81 0.025 0.0 0.0 0.0 0.0 82-83 0.0875 0.0 0.0 0.0 0.0 84-85 0.1125 0.0 0.0 0.0 0.0 86-87 0.21250000000000002 0.0 0.0 0.0 0.0 88-89 0.2625 0.0 0.0 0.0 0.0 90-91 0.2875 0.0 0.0 0.0 0.0 92-93 0.35 0.0 0.0 0.0 0.0 94-95 0.475 0.0 0.0 0.0 0.0 96-97 0.625 0.0 0.0 0.0 0.0 98-99 0.75 0.0 0.0 0.0 0.0 100-101 0.925 0.0 0.0 0.0 0.0 102-103 1.075 0.0 0.0 0.0 0.0 104-105 1.275 0.0 0.0 0.0 0.0 106-107 1.6 0.0 0.0 0.0 0.0 108-109 1.8375 0.0 0.0 0.0 0.0 110-111 2.175 0.0 0.0 0.0 0.0 112-113 2.425 0.0 0.0 0.0 0.0 114-115 2.7249999999999996 0.0 0.0 0.0 0.0 116-117 3.1125 0.0 0.0 0.0 0.0 118-119 3.5375 0.0 0.0 0.0 0.0 120-121 4.0 0.0 0.0 0.0 0.0 122-123 4.3625 0.0 0.0 0.0 0.0 124-125 4.8 0.0 0.0 0.0 0.0 126-127 5.2 0.0 0.0 0.0 0.0 128-129 5.825 0.0 0.0 0.0 0.0 130-131 6.425000000000001 0.0 0.0 0.0 0.0 132-133 7.025 0.0 0.0 0.0 0.0 134-135 7.8625 0.0 0.0 0.0 0.0 136-137 8.7 0.0 0.0 0.0 0.0 138-139 9.4875 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACTTGTA 10 0.006841402 144.925 5 CATTCTT 10 0.006841402 144.925 9 TTGGTTC 10 0.006841402 144.925 3 >>END_MODULE SRR6941600 read2 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR6941600_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 33.08375 33.0 33.0 34.0 32.0 34.0 2 33.18825 34.0 33.0 34.0 33.0 34.0 3 33.206 34.0 33.0 34.0 33.0 34.0 4 33.1805 34.0 33.0 34.0 33.0 34.0 5 33.177 34.0 33.0 34.0 33.0 34.0 6 37.3655 38.0 38.0 38.0 37.0 38.0 7 37.347 38.0 38.0 38.0 37.0 38.0 8 37.36975 38.0 38.0 38.0 37.0 38.0 9 37.3215 38.0 38.0 38.0 37.0 38.0 10-14 37.11635 38.0 38.0 38.0 36.8 38.0 15-19 37.2504 38.0 38.0 38.0 36.8 38.0 20-24 37.329449999999994 38.0 38.0 38.0 37.2 38.0 25-29 37.263850000000005 38.0 38.0 38.0 37.0 38.0 30-34 36.97885 38.0 38.0 38.0 36.4 38.0 35-39 36.8326 38.0 38.0 38.0 36.0 38.0 40-44 37.18095 38.0 38.0 38.0 37.0 38.0 45-49 37.24565 38.0 38.0 38.0 37.0 38.0 50-54 37.2268 38.0 38.0 38.0 37.0 38.0 55-59 37.14695 38.0 38.0 38.0 37.0 38.0 60-64 37.2153 38.0 38.0 38.0 37.0 38.0 65-69 37.0774 38.0 38.0 38.0 36.4 38.0 70-74 36.8942 38.0 38.0 38.0 35.8 38.0 75-79 37.031949999999995 38.0 38.0 38.0 36.2 38.0 80-84 36.81665 38.0 38.0 38.0 35.6 38.0 85-89 36.5874 38.0 38.0 38.0 34.6 38.0 90-94 36.9175 38.0 38.0 38.0 36.0 38.0 95-99 36.812 38.0 38.0 38.0 35.4 38.0 100-104 35.927550000000004 38.0 37.2 38.0 30.8 38.0 105-109 36.188849999999995 38.0 37.2 38.0 32.8 38.0 110-114 36.617149999999995 38.0 38.0 38.0 35.0 38.0 115-119 36.555099999999996 38.0 38.0 38.0 34.6 38.0 120-124 36.23159999999999 38.0 37.6 38.0 33.0 38.0 125-129 35.902750000000005 38.0 37.4 38.0 32.6 38.0 130-134 35.97964999999999 38.0 37.6 38.0 33.4 38.0 135-139 32.79965 37.4 30.0 38.0 21.2 38.0 140-144 34.07165 37.6 33.0 38.0 26.6 38.0 145-149 34.90135 38.0 36.0 38.0 31.0 38.0 150-151 30.57275 35.5 29.5 38.0 16.5 38.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5.0 3 6.0 4 2.0 5 3.0 6 1.0 7 1.0 8 1.0 9 0.0 10 1.0 11 0.0 12 0.0 13 1.0 14 0.0 15 1.0 16 2.0 17 2.0 18 3.0 19 2.0 20 2.0 21 3.0 22 3.0 23 4.0 24 2.0 25 8.0 26 16.0 27 16.0 28 22.0 29 29.0 30 35.0 31 38.0 32 80.0 33 90.0 34 122.0 35 247.0 36 742.0 37 2510.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 37.525 19.55 15.875 27.05 2 27.52064048036027 19.714786089567177 35.126344758568926 17.63822867150363 3 20.485850237916353 23.29075882794891 37.13999499123466 19.083395942900076 4 23.654568210262827 32.94117647058823 25.90738423028786 17.496871088861077 5 24.76214321482223 32.54882323485228 24.887330996494743 17.801702553830744 6 20.4 33.0 26.875 19.725 7 17.8 19.3 42.5 20.4 8 21.375 22.275 30.3 26.05 9 23.1 21.675 33.074999999999996 22.15 10-14 25.31 25.455 28.51 20.724999999999998 15-19 24.47 25.21 29.615000000000002 20.705000000000002 20-24 25.295 25.145 29.865000000000002 19.695 25-29 24.4736710506576 25.51382707406111 29.344401660249037 20.668100215032254 30-34 25.230000000000004 24.925 29.23 20.615 35-39 24.325 25.8 29.285 20.59 40-44 23.655 26.005 29.4 20.94 45-49 23.505000000000003 26.979999999999997 28.310000000000002 21.205 50-54 23.810000000000002 25.759999999999998 29.235 21.195 55-59 23.474999999999998 25.979999999999997 29.32 21.224999999999998 60-64 24.015 25.285000000000004 29.459999999999997 21.240000000000002 65-69 24.37 25.615 28.83 21.185000000000002 70-74 24.91122780695174 25.191297824456115 29.56739184796199 20.330082520630157 75-79 24.265 25.72 29.67 20.345 80-84 25.040000000000003 24.740000000000002 30.005 20.215 85-89 25.195 26.07 28.09 20.645 90-94 24.44 25.5 28.92 21.14 95-99 24.474999999999998 25.205 29.110000000000003 21.21 100-104 25.275 25.81 29.220000000000002 19.695 105-109 25.16 24.22 29.26 21.36 110-114 24.365000000000002 25.21 28.9 21.525 115-119 24.4 25.795 29.085 20.72 120-124 24.715 26.395000000000003 27.415 21.475 125-129 24.610000000000003 26.790000000000003 27.165 21.435000000000002 130-134 25.185000000000002 26.57 28.355000000000004 19.89 135-139 24.185000000000002 27.115000000000002 28.560000000000002 20.14 140-144 25.495 26.150000000000002 28.634999999999998 19.72 145-149 25.335 26.235000000000003 28.185 20.244999999999997 150-151 25.324999999999996 25.95 29.462500000000002 19.2625 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.5 18 1.0 19 1.0 20 1.5 21 3.0 22 4.0 23 4.0 24 7.5 25 12.0 26 14.0 27 13.0 28 13.5 29 21.5 30 27.0 31 27.5 32 29.5 33 47.5 34 70.5 35 73.0 36 93.0 37 139.0 38 186.5 39 190.0 40 213.5 41 224.0 42 202.5 43 231.0 44 233.5 45 195.0 46 172.0 47 143.5 48 114.0 49 95.0 50 73.0 51 77.0 52 68.0 53 78.0 54 104.0 55 110.5 56 106.5 57 84.0 58 79.5 59 96.5 60 79.0 61 55.0 62 54.0 63 34.5 64 16.5 65 14.5 66 12.0 67 11.5 68 10.5 69 8.5 70 7.5 71 5.0 72 1.5 73 2.0 74 2.5 75 2.0 76 2.0 77 0.5 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.075 3 0.17500000000000002 4 0.125 5 0.15 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.015 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.025 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 70.875 #Duplication Level Percentage of deduplicated Percentage of total 1 83.91534391534393 59.475 2 8.430335097001764 11.95 3 2.7865961199294533 5.925 4 1.6225749559082892 4.6 5 0.9523809523809524 3.375 6 0.6701940035273368 2.85 7 0.28218694885361556 1.4000000000000001 8 0.3880070546737213 2.1999999999999997 9 0.3880070546737213 2.475 >10 0.5643738977072311 5.75 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source ATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAA 28 0.7000000000000001 No Hit ATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCA 27 0.675 No Hit GCACAACATCCTTATGCATCCATTTCACATGTTAGGTGTAGCTGGTGTTT 16 0.4 No Hit GTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGATCTT 15 0.375 No Hit GCTTGTTATATGGGTCGTGAGTGGGAACTTAGTTTCCGTTTGGGTATGCG 15 0.375 No Hit GGAAGCTTCTCTGATGGTATGCCTTTAGGAATATCTGGTACTTTCAACTT 15 0.375 No Hit GTTCGATCCTGGCTCAGGATGAACGCTGGCGGCATGCTTAACACATGCAA 13 0.325 No Hit TATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAA 12 0.3 No Hit TTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAG 12 0.3 No Hit CTATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAA 12 0.3 No Hit GGTACTTTCAACTTTATGATTGTATTCCAGGCAGAGCACAACATCCTTAT 12 0.3 No Hit CTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGAT 11 0.27499999999999997 No Hit CTGGTACTTTCAACTTTATGATTGTATTCCAGGCAGAGCACAACATCCTT 11 0.27499999999999997 No Hit CTTCCAATATGCTAGTTTCAACAACTCTCGTTCTTTACACTTCTTCTTGG 11 0.27499999999999997 No Hit GCTAATTGTTCTACACTTCTTACTTGGTGTAGCTTGTTATATGGGTCGTG 10 0.25 No Hit GGGAACTTAGTTTCCGTTTGGGTATGCGCCCTTGGATTGCTGTTGCATAT 10 0.25 No Hit TTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAA 9 0.22499999999999998 No Hit GGAAACTACTGAAAATGAATCTGCTAATGAGGGTTACAAATTTGGTCAAG 9 0.22499999999999998 No Hit CCAAGATCTTACCATGACTGCAATTTTAGAGAGACGCGAAAGTACAAGCC 9 0.22499999999999998 No Hit CCTGGCTCAGGATGAACGCTGGCGGCATGCTTAACACATGCAAGTCGAAC 9 0.22499999999999998 No Hit AGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGATCT 9 0.22499999999999998 No Hit ATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCTAAATGG 9 0.22499999999999998 No Hit CATCAACCGTGCTAATCTTGGTATGGAAGTAATGCACGAACGTAATGCTC 9 0.22499999999999998 No Hit GCTGCGACTGCTGTTTTCTTGATTTACCCTATTGGTCAAGGAAGCTTCTC 9 0.22499999999999998 No Hit GCTGCATCCGTTGATGAATGGTTATACAATGGTGGTCCTTATGAGCTAAT 9 0.22499999999999998 No Hit ATTATCTATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAAT 9 0.22499999999999998 No Hit GTTCAACCTAAATGGATTCAATTTCAACCAATCTGTAGTTGATAGTCAAG 9 0.22499999999999998 No Hit AGCACAACATCCTTATGCATCCATTTCACATGTTAGGTGTAGCTGGTGTT 8 0.2 No Hit CCTAAATGGATTCAATTTCAACCAATCTGTAGTTGATAGTCAAGGTCGCG 8 0.2 No Hit ATCGCCTTCATCGCAGCCCCTCCAGTAGATATTGATGGTATTCGCGAGCC 8 0.2 No Hit CATGACTGCAATTTTAGAGAGACGCGAAAGTACAAGCCTGTGGGGTCGCT 8 0.2 No Hit GTTGCATATTCAGCTCCTGTTGCAGCTGCGACTGCTGTTTTCTTGATTTA 8 0.2 No Hit CAATGGTGGTCCTTATGAGCTAATTGTTCTACACTTCTTACTTGGTGTAG 8 0.2 No Hit GTCAAGGTCGCGTTATTAATACTTGGGCTGATATCATCAACCGTGCTAAT 8 0.2 No Hit GTTAGGTGTAGCTGGTGTTTTCGGCGGTTCCCTATTCAGTGCTATGCATG 8 0.2 No Hit CCTTACCAGGGCTTGACATGCCGCGAATCCTCTTGAAAGAGAGGGGTGCC 8 0.2 No Hit TATCTATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTT 8 0.2 No Hit GTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGATCTTACCATGACTG 8 0.2 No Hit GGGAAACTACTGAAAATGAATCTGCTAATGAGGGTTACAAATTTGGTCAA 7 0.17500000000000002 No Hit TAGGAATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCTA 7 0.17500000000000002 No Hit TGGGAACTTAGTTTCCGTTTGGGTATGCGCCCTTGGATTGCTGTTGCATA 7 0.17500000000000002 No Hit GGAATATCTGGTACTTTCAACTTTATGATTGTATTCCAGGCAGAGCACAA 7 0.17500000000000002 No Hit CTTTACTTTATGGAAACAATATTATCTCTGGTGCTATTATTCCTACTTCT 7 0.17500000000000002 No Hit CCTATTCAGTGCTATGCATGGTTCCTTGGTAACCTCTAGTTTGATCAGGG 7 0.17500000000000002 No Hit GGCTTGCGGTGGATACCTAGGCACCCAGAGACGAGGAAGGGCGTAGCAAG 7 0.17500000000000002 No Hit CGCGAAAGTACAAGCCTGTGGGGTCGCTTCTGCAACTGGATAACTAGCAC 7 0.17500000000000002 No Hit GGTCGCTTCTGCAACTGGATAACTAGCACTGAAAATCGTCTTTACATCGG 6 0.15 No Hit GGTAACCTCTAGTTTGATCAGGGAAACTACTGAAAATGAATCTGCTAATG 6 0.15 No Hit TATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAAT 6 0.15 No Hit GCGAGAGCTTGTAACCCGAGTGGGGGCTTTAAGGGTGGTGTGGACGTTGC 6 0.15 No Hit TGAAAATGAATCTGCTAATGAGGGTTACAAATTTGGTCAAGAGGAAGAGA 6 0.15 No Hit CGCAGCCCCTCCAGTAGATATTGATGGTATTCGCGAGCCTGTTTCTGGTT 6 0.15 No Hit GTGACCAGGCCCGGGCGACTGTTTACCAAAAACACAGGTCTCCGCAAAGT 6 0.15 No Hit TGAAAATCGTCTTTACATCGGATGGTTCGGTGTTTTGATGATCCCTACCT 6 0.15 No Hit CGCGAGCCTGTTTCTGGTTCTTTACTTTATGGAAACAATATTATCTCTGG 6 0.15 No Hit CTTAGTTTCCGTTTGGGTATGCGCCCTTGGATTGCTGTTGCATATTCAGC 6 0.15 No Hit TCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGA 6 0.15 No Hit TGATGGTATTCGCGAGCCTGTTTCTGGTTCTTTACTTTATGGAAACAATA 6 0.15 No Hit GTTTCCGTTTGGGTATGCGCCCTTGGATTGCTGTTGCATATTCAGCTCCT 6 0.15 No Hit TTTTGGCCGATTAATCTTCCAATATGCTAGTTTCAACAACTCTCGTTCTT 6 0.15 No Hit GGCCGATTAATCTTCCAATATGCTAGTTTCAACAACTCTCGTTCTTTACA 6 0.15 No Hit TCTATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGA 6 0.15 No Hit GTTTGATCAGGGAAACTACTGAAAATGAATCTGCTAATGAGGGTTACAAA 6 0.15 No Hit ATCTATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTG 6 0.15 No Hit CAACATCCTTATGCATCCATTTCACATGTTAGGTGTAGCTGGTGTTTTCG 6 0.15 No Hit CTTTTACCCAATTTGGGAAGCTGCATCCGTTGATGAATGGTTATACAATG 5 0.125 No Hit CCGCAACTTCTGTATTTATTATCGCCTTCATCGCAGCCCCTCCAGTAGAT 5 0.125 No Hit GTGAAATCAAGGGGCATTACTTGAATGCAACTGCGGGTACATGTGAAGAA 5 0.125 No Hit CTCGTTCTTTACACTTCTTCTTGGCTGCTTGGCCTGTAGTAGGAATCTGG 5 0.125 No Hit GAATCTGCTAATGAGGGTTACAAATTTGGTCAAGAGGAAGAGACTTATAA 5 0.125 No Hit GTCTTTACATCGGATGGTTCGGTGTTTTGATGATCCCTACCTTATTGACC 5 0.125 No Hit CTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCTAAATGGAT 5 0.125 No Hit CTTGGATTGCTGTTGCATATTCAGCTCCTGTTGCAGCTGCGACTGCTGTT 5 0.125 No Hit ATTTGGTCAAGAGGAAGAGACTTATAATATTGTGGCTGCTCATGGTTATT 5 0.125 No Hit AATGAATCTGCTAATGAGGGTTACAAATTTGGTCAAGAGGAAGAGACTTA 5 0.125 No Hit GTTATTTTGGCCGATTAATCTTCCAATATGCTAGTTTCAACAACTCTCGT 5 0.125 No Hit GTTATACAATGGTGGTCCTTATGAGCTAATTGTTCTACACTTCTTACTTG 5 0.125 No Hit TTCCAATATGCTAGTTTCAACAACTCTCGTTCTTTACACTTCTTCTTGGC 5 0.125 No Hit CTAGCACTGAAAATCGTCTTTACATCGGATGGTTCGGTGTTTTGATGATC 5 0.125 No Hit GGAATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCTAAA 5 0.125 No Hit GTGGGAACTTAGTTTCCGTTTGGGTATGCGCCCTTGGATTGCTGTTGCAT 5 0.125 No Hit ATTCAGTGCTATGCATGGTTCCTTGGTAACCTCTAGTTTGATCAGGGAAA 5 0.125 No Hit TGTAGCTGGTGTTTTCGGCGGTTCCCTATTCAGTGCTATGCATGGTTCCT 5 0.125 No Hit TGGCGTTCAACCTAAATGGATTCAATTTCAACCAATCTGTAGTTGATAGT 5 0.125 No Hit CCTAGGCACCCAGAGACGAGGAAGGGCGTAGCAAGCGACGAAATGCTTCG 5 0.125 No Hit CGGATGGTTCGGTGTTTTGATGATCCCTACCTTATTGACCGCAACTTCTG 5 0.125 No Hit AGGGAAACTACTGAAAATGAATCTGCTAATGAGGGTTACAAATTTGGTCA 5 0.125 No Hit TGCAATTTGAATAAACCAAGATCTTACCATGACTGCAATTTTAGAGAGAC 5 0.125 No Hit CGGCAATCGGATTGCACTTTTACCCAATTTGGGAAGCTGCATCCGTTGAT 5 0.125 No Hit CGCAACTTCTGTATTTATTATCGCCTTCATCGCAGCCCCTCCAGTAGATA 5 0.125 No Hit GCCGATTAATCTTCCAATATGCTAGTTTCAACAACTCTCGTTCTTTACAC 5 0.125 No Hit TTCAGCTCCTGTTGCAGCTGCGACTGCTGTTTTCTTGATTTACCCTATTG 5 0.125 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.0125 0.0 0.0 0.0 0.0 70-71 0.025 0.0 0.0 0.0 0.0 72-73 0.025 0.0 0.0 0.0 0.0 74-75 0.025 0.0 0.0 0.0 0.0 76-77 0.025 0.0 0.0 0.0 0.0 78-79 0.025 0.0 0.0 0.0 0.0 80-81 0.025 0.0 0.0 0.0 0.0 82-83 0.0875 0.0 0.0 0.0 0.0 84-85 0.1125 0.0 0.0 0.0 0.0 86-87 0.21250000000000002 0.0 0.0 0.0 0.0 88-89 0.2625 0.0 0.0 0.0 0.0 90-91 0.2875 0.0 0.0 0.0 0.0 92-93 0.375 0.0 0.0 0.0 0.0 94-95 0.5 0.0 0.0 0.0 0.0 96-97 0.65 0.0 0.0 0.0 0.0 98-99 0.7749999999999999 0.0 0.0 0.0 0.0 100-101 0.95 0.0 0.0 0.0 0.0 102-103 1.075 0.0 0.0 0.0 0.0 104-105 1.275 0.0 0.0 0.0 0.0 106-107 1.6 0.0 0.0 0.0 0.0 108-109 1.8375 0.0 0.0 0.0 0.0 110-111 2.1500000000000004 0.0 0.0 0.0 0.0 112-113 2.4000000000000004 0.0 0.0 0.0 0.0 114-115 2.7 0.0 0.0 0.0 0.0 116-117 3.1 0.0 0.0 0.0 0.0 118-119 3.5375 0.0 0.0 0.0 0.0 120-121 4.0 0.0 0.0 0.0 0.0 122-123 4.3625 0.0 0.0 0.0 0.0 124-125 4.75 0.0 0.0 0.0 0.0 126-127 5.1 0.0 0.0 0.0 0.0 128-129 5.6 0.0 0.0 0.0 0.0 130-131 6.1 0.0 0.0 0.0 0.0 132-133 6.5375 0.0 0.0 0.0 0.0 134-135 7.137499999999999 0.0 0.0 0.0 0.0 136-137 7.8375 0.0 0.0 0.0 0.0 138-139 8.524999999999999 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TGAGAAT 10 0.006830828 145.0 8 TGTGAGA 10 0.006830828 145.0 6 GAGAATT 10 0.006830828 145.0 9 ATTGTGA 10 0.006830828 145.0 4 TTGTGAG 10 0.006830828 145.0 5 >>END_MODULE Read 1065304 spots for SRR6941600.sra Written 1065304 spots for SRR6941600.sra Read 1065304 spots for SRR6941600.sra Written 1065304 spots for SRR6941600.sra Read 1065304 spots for SRR6941600.sra Written 1065304 spots for SRR6941600.sra Read 1065304 spots for SRR6941600.sra Written 1065304 spots for SRR6941600.sra Read 1065304 spots for SRR6941600.sra Written 1065304 spots for SRR6941600.sra Read 1065304 spots for SRR6941600.sra Written 1065304 spots for SRR6941600.sra Read 1065304 spots for SRR6941600.sra Written 1065304 spots for SRR6941600.sra Read 1065304 spots for SRR6941600.sra Written 1065304 spots for SRR6941600.sra Read 1065304 spots for SRR6941600.sra Written 1065304 spots for SRR6941600.sra Read 1065304 spots for SRR6941600.sra Written 1065304 spots for SRR6941600.sra Read 1065318 spots for SRR6941600.sra Written 1065318 spots for SRR6941600.sra Read 1065304 spots for SRR6941600.sra Written 1065304 spots for SRR6941600.sra Read 1065304 spots for SRR6941600.sra Written 1065304 spots for SRR6941600.sra Read 1065304 spots for SRR6941600.sra Written 1065304 spots for SRR6941600.sra Read 1065304 spots for SRR6941600.sra Written 1065304 spots for SRR6941600.sra Read 1065304 spots for SRR6941600.sra Written 1065304 spots for SRR6941600.sra Read 1065304 spots for SRR6941600.sra Written 1065304 spots for SRR6941600.sra Read 1065304 spots for SRR6941600.sra Written 1065304 spots for SRR6941600.sra Read 1065304 spots for SRR6941600.sra Written 1065304 spots for SRR6941600.sra Read 1065304 spots for SRR6941600.sra Written 1065304 spots for SRR6941600.sra SRR ids: ['SRR6941600.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_8a4pozd_ SRR6941600.sra spots: 21306094 blocks: [[1, 1065304], [1065305, 2130608], [2130609, 3195912], [3195913, 4261216], [4261217, 5326520], [5326521, 6391824], [6391825, 7457128], [7457129, 8522432], [8522433, 9587736], [9587737, 10653040], [10653041, 11718344], [11718345, 12783648], [12783649, 13848952], [13848953, 14914256], [14914257, 15979560], [15979561, 17044864], [17044865, 18110168], [18110169, 19175472], [19175473, 20240776], [20240777, 21306094]] SRR6941600 file size 7198235 SRR6941600 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR6941600 SRR6941600_1.fastq SRR6941600_2.fastq Input file: SRR6941600_1.fastq Paired file: SRR6941600_2.fastq trimmed: SRR6941600-trimmed-pair1.fastq, SRR6941600-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Fri Dec 6 12:44:39 2024 >> started Fri Dec 6 12:45:03 2024 >> done (23.884s) 21306094 read pairs processed; of these: 30085 ( 0.14%) short read pairs filtered out after trimming by size control 21279 ( 0.10%) empty read pairs filtered out after trimming by size control 21254730 (99.76%) read pairs available; of these: 8049546 (37.87%) trimmed read pairs available after processing 13205184 (62.13%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 1 0.00% 19 5 0.00% 20 1 0.00% 21 2 0.00% 22 0 0.00% 23 2 0.00% 24 1 0.00% 25 5 0.00% 26 12 0.00% 27 24 0.00% 28 5 0.00% 29 1 0.00% 30 7 0.00% 31 2 0.00% 32 3 0.00% 33 5 0.00% 34 7 0.00% 35 7 0.00% 36 10 0.00% 37 7 0.00% 38 8 0.00% 39 6 0.00% 40 15 0.00% 41 16 0.00% 42 12 0.00% 43 22 0.00% 44 22 0.00% 45 20 0.00% 46 21 0.00% 47 25 0.00% 48 24 0.00% 49 47 0.00% 50 45 0.00% 51 58 0.00% 52 81 0.00% 53 62 0.00% 54 82 0.00% 55 101 0.00% 56 99 0.00% 57 110 0.00% 58 151 0.00% 59 150 0.00% 60 205 0.00% 61 218 0.00% 62 323 0.00% 63 345 0.00% 64 409 0.00% 65 437 0.00% 66 490 0.00% 67 503 0.00% 68 644 0.00% 69 682 0.00% 70 869 0.00% 71 954 0.00% 72 1193 0.01% 73 1362 0.01% 74 1547 0.01% 75 1774 0.01% 76 1819 0.01% 77 2038 0.01% 78 2360 0.01% 79 2754 0.01% 80 3171 0.01% 81 3644 0.02% 82 4162 0.02% 83 4833 0.02% 84 6346 0.03% 85 7913 0.04% 86 8155 0.04% 87 8529 0.04% 88 9707 0.05% 89 9672 0.05% 90 10365 0.05% 91 11059 0.05% 92 13249 0.06% 93 14242 0.07% 94 15151 0.07% 95 16847 0.08% 96 16448 0.08% 97 17887 0.08% 98 19100 0.09% 99 20313 0.10% 100 20856 0.10% 101 22935 0.11% 102 24498 0.12% 103 25563 0.12% 104 27659 0.13% 105 29168 0.14% 106 29633 0.14% 107 31572 0.15% 108 32344 0.15% 109 33592 0.16% 110 34615 0.16% 111 36929 0.17% 112 38845 0.18% 113 39754 0.19% 114 44379 0.21% 115 47292 0.22% 116 49324 0.23% 117 49216 0.23% 118 48500 0.23% 119 48273 0.23% 120 49677 0.23% 121 53680 0.25% 122 60985 0.29% 123 60133 0.28% 124 63586 0.30% 125 66604 0.31% 126 63960 0.30% 127 66308 0.31% 128 66642 0.31% 129 68943 0.32% 130 65107 0.31% 131 70194 0.33% 132 75336 0.35% 133 73621 0.35% 134 80447 0.38% 135 79241 0.37% 136 82610 0.39% 137 83213 0.39% 138 89045 0.42% 139 91857 0.43% 140 94321 0.44% 141 108154 0.51% 142 104768 0.49% 143 115185 0.54% 144 124786 0.59% 145 148269 0.70% 146 164795 0.78% 147 201402 0.95% 148 291513 1.37% 149 543537 2.56% 150 3783677 17.80% 151 13205184 62.13% 21254730 reads passed initial QC criterion=sequence-density sequence-density=0.43 sequence-density-rank=1 fanout-score=2.40 fanout-score-rank=27 prefix-density=0.44 prefix-fanout=2.4 sequence=GTTACGGCCGCCGTTCACCGGGGCTTCGGTCGCCGGCTTCCCTGTCATCAGTTCACCAACTTCCTTGACCTTCCGGCACTGGGCAGGCGTCAGCCCCCATACATGGTCTTACGACTTTGCGGAGACCTGTGTTTTTGGTAAACAGTCGCCCGGGCCTGGTCACTG criterion=fanout-score sequence-density=0.01 sequence-density-rank=38 fanout-score=73.68 fanout-score-rank=1 prefix-density=0.28 prefix-fanout=2.9 sequence=AGAAAAAAACAAGTTTGCATCTTCAGGAGAATCTATATTTTCGCGAAATGGATCATAATAAAATGGATTTTAGGTATCTAGGGAAAATTCACTTCGAAGTAACTATTTCCTAGATACCTATGCACGGTACTTCACGGTTGAATGAATCAACCTGAAAAATACCTAAAAAAGGCCTAAAGTTAAGGATTTATCAATGGGTAATGTTGCTCCAATACCTAACCAAAGAGCTACTGCAGTACCGATTAAAAAAACGGTCGTAGCTACTGGGCGACGAAATGGATTTTGGAATTTGTTGACATTCTCTAGAAAAGGTACTGTCAATAAGCCTGTTGGCACAGAAACCATTAAGAGAACGCCCAATAACTTATTGGGTACCGTACGGAGTATTTGAAACACGGGAAAGAAGTACCACTCGGGTAATATTTCCAAAGGAGTTGCAAACGGATCCGCGGGTTCACCAATCATTGATGGCTCGAGAACAGCTAAACCTACATTACATGCAATAGTACCT criterion=sequence-density sequence-density=0.36 sequence-density-rank=1 fanout-score=2.87 fanout-score-rank=26 prefix-density=1.05 prefix-fanout=1.0 sequence=TTGCGTAGTGGATCTGCTGGGGCCTATGCGAAAGCTGGGCCTCACGGATCCTAGAGCGGCAGGCACCGCGTGAGGCTG criterion=fanout-score sequence-density=0.01 sequence-density-rank=47 fanout-score=26.73 fanout-score-rank=1 prefix-density=0.04 prefix-fanout=3.2 sequence=GGATTTGTTAAATCAAATCCTTGGTTTAATAACGAACGGTGTTAACTTACCATAACAACAACTCAATTCCTATCGAATTCCTATAGTGAAATTCCTATAGGATAGAACATACACAGGGTGTACGCATTATATATGAATGAAAAATATTAATTAACTTAAGCATGCCCTCAATTTTCTGTAATGAGTTGCTATTAATTGAATATCTCTTTTTTTTTATGAGATTTTTGCTAAAGTTTCATTTACGCCTAATTCACATCGAGTAGACCTTGTTATTGTGAGAATTCTTAATTCAAGAGTTGTAAGGAGGGACTTATGTCACCACAAACAGAAACTAAAGCAAGTGTTGGATTTAAAGCTGGTGTTAAAGATTATAGATTGACTTACTACACCCCGGAGTATGAAACCAAGGATACTGATATCTTGGCAGCATTCCGAGTATCTCCTCAACCTGGGGTTCCGCCCGAAGAAGCAGGGGCTGCAGTAGCTGCCGAATCTTCTACTGGTACATGGA SRR6941600 testing PE reads STAR mapping to Ensembl genome Started job on | Dec 06 12:45:53 Started mapping on | Dec 06 12:45:53 Finished on | Dec 06 12:48:01 Mapping speed, Million of reads per hour | 597.79 Number of input reads | 21254730 Average input read length | 294 UNIQUE READS: Uniquely mapped reads number | 12458861 Uniquely mapped reads % | 58.62% Average mapped length | 295.22 Number of splices: Total | 2377886 Number of splices: Annotated (sjdb) | 2144241 Number of splices: GT/AG | 2262892 Number of splices: GC/AG | 27585 Number of splices: AT/AC | 8329 Number of splices: Non-canonical | 79080 Mismatch rate per base, % | 0.29% Deletion rate per base | 0.02% Deletion average length | 1.95 Insertion rate per base | 0.02% Insertion average length | 2.39 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 7306363 % of reads mapped to multiple loci | 34.38% Number of reads mapped to too many loci | 100208 % of reads mapped to too many loci | 0.47% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 3.91% % of reads unmapped: other | 2.63% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 1501130 1501130 1501130 N_multimapping 7306363 7306363 7306363 N_noFeature 5530095 12042983 5696459 N_ambiguous 468914 7229 225324 UnstrandedReadsAssigned:6459852 PositiveStrandReadsAssigned:408649 NegativeStrandReadsAssigned:6537078 Dataset is classified negative stranded MeadianReadLen=151 20thPercentileLength=150 echo kmer=145 SRR6941600 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,972 [index] number of k-mers: 66,720,672 [index] number of equivalence classes: 111,837 [quant] running in paired-end mode [quant] will process pair 1: SRR6941600-trimmed-pair1.fastq SRR6941600-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 21,254,730 reads, 10,639,903 reads pseudoaligned [quant] estimated average fragment length: 226.496 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,071 rounds 52973 SRR6941600.ke.tsv 35125 SRR6941600.se.tsv 88098 total ==> SRR6941600.ke.tsv <== target_id length eff_length est_counts tpm PNS24245 936 710.986 0 0 PNS24247 1044 818.504 8.97709 0.876165 PNS24249 1928 1702.5 2.61882 0.122882 PNS24246 1044 818.504 8.97709 0.876165 PNS24248 1044 818.504 8.97709 0.876165 PNS24244 1471 1245.5 6.44992 0.413695 PNS24243 293 106.318 0 0 KQK14069 1603 1377.5 304.951 17.6851 KQK14071 474 259.275 5.94152 1.83066 ==> SRR6941600.se.tsv <== BRADI_1g14170v3 406 BRADI_1g53295v3 45 BRADI_1g59795v3 13 BRADI_1g07683v3 0 BRADI_1g00485v3 0 BRADI_1g20270v3 93 BRADI_1g74790v3 15 BRADI_1g09890v3 0 BRADI_1g77505v3 35 BRADI_1g48960v3 0 SRR6941600 completed mapping pipeline successfully