Starting /dee2/code/volunteer_pipeline.sh SRR6941601 current disk space = 1551345434624 free memory = 1328105412 SRR6941601 SRAfilesize 63863271c995db3f1e3a0ce5479952d1 SRR6941601.sra SRR6941601.sra file validated SRR6941601 is paired end SRR6941601 is conventional basespace SRR6941601 read1 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR6941601_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 26.96425 32.0 18.0 33.0 18.0 33.0 2 30.1265 31.0 29.0 33.0 25.0 33.0 3 30.82375 31.0 29.0 33.0 27.0 33.0 4 30.67125 31.0 29.0 33.0 27.0 33.0 5 32.3175 33.0 33.0 33.0 32.0 33.0 6 36.6355 38.0 37.0 38.0 34.0 38.0 7 37.2195 38.0 38.0 38.0 36.0 38.0 8 37.604 38.0 38.0 38.0 37.0 38.0 9 37.6765 38.0 38.0 38.0 38.0 38.0 10-14 37.5149 38.0 38.0 38.0 37.8 38.0 15-19 37.41065 38.0 38.0 38.0 37.4 38.0 20-24 37.52485 38.0 38.0 38.0 38.0 38.0 25-29 37.639250000000004 38.0 38.0 38.0 38.0 38.0 30-34 37.652699999999996 38.0 38.0 38.0 38.0 38.0 35-39 37.59845 38.0 38.0 38.0 38.0 38.0 40-44 37.56555 38.0 38.0 38.0 37.8 38.0 45-49 37.5366 38.0 38.0 38.0 37.6 38.0 50-54 37.39005 38.0 38.0 38.0 37.2 38.0 55-59 37.2954 38.0 38.0 38.0 36.8 38.0 60-64 37.4124 38.0 38.0 38.0 37.0 38.0 65-69 37.3269 38.0 38.0 38.0 36.8 38.0 70-74 37.38275 38.0 38.0 38.0 37.0 38.0 75-79 37.32 38.0 38.0 38.0 36.8 38.0 80-84 37.4014 38.0 38.0 38.0 37.0 38.0 85-89 37.27575 38.0 38.0 38.0 36.4 38.0 90-94 36.35275 38.0 37.2 38.0 30.6 38.0 95-99 36.885400000000004 38.0 37.8 38.0 35.2 38.0 100-104 37.14635 38.0 38.0 38.0 36.0 38.0 105-109 37.058049999999994 38.0 38.0 38.0 35.8 38.0 110-114 37.010149999999996 38.0 38.0 38.0 35.4 38.0 115-119 36.824650000000005 38.0 38.0 38.0 34.8 38.0 120-124 36.72149999999999 38.0 38.0 38.0 34.6 38.0 125-129 36.62545 38.0 38.0 38.0 34.4 38.0 130-134 36.6627 38.0 38.0 38.0 34.8 38.0 135-139 36.390049999999995 38.0 38.0 38.0 34.0 38.0 140-144 36.2293 38.0 38.0 38.0 33.8 38.0 145-149 35.81755 38.0 37.4 38.0 33.0 38.0 150-151 33.065 37.0 33.5 38.0 16.5 38.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 19 3.0 20 2.0 21 2.0 22 2.0 23 3.0 24 1.0 25 2.0 26 6.0 27 11.0 28 12.0 29 17.0 30 27.0 31 38.0 32 54.0 33 78.0 34 101.0 35 201.0 36 517.0 37 2923.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 31.81093998937865 9.559214020180564 9.028146574614976 49.60169941582581 2 23.025000000000002 15.225 33.925 27.825 3 20.95 17.325 24.099999999999998 37.625 4 27.400000000000002 25.5 21.099999999999998 26.0 5 23.875 31.424999999999997 22.325 22.375 6 20.674999999999997 32.05 24.925 22.35 7 17.325 21.625 40.375 20.674999999999997 8 19.975 19.025 30.8 30.2 9 19.075 19.825 33.825 27.275 10-14 22.11 26.245 23.605 28.04 15-19 22.31 24.625 26.015 27.05 20-24 22.405 25.650000000000002 25.97 25.974999999999998 25-29 23.365 24.11 25.985000000000003 26.540000000000003 30-34 23.04 24.68 25.545 26.735 35-39 22.27 25.055 25.91 26.765 40-44 23.44 23.555 26.284999999999997 26.72 45-49 21.36 24.085 27.975 26.58 50-54 22.564999999999998 24.645 25.740000000000002 27.05 55-59 21.959999999999997 24.740000000000002 25.650000000000002 27.650000000000002 60-64 22.439999999999998 24.285 26.119999999999997 27.155 65-69 22.439999999999998 24.735 25.014999999999997 27.810000000000002 70-74 23.150000000000002 25.169999999999998 24.25 27.43 75-79 22.61 25.130000000000003 24.73 27.529999999999998 80-84 23.62 25.115 24.485 26.779999999999998 85-89 22.405 24.18 26.47 26.945000000000004 90-94 22.235 24.9 25.290000000000003 27.575 95-99 21.525 25.365 25.275 27.834999999999997 100-104 22.95 24.95 24.85 27.250000000000004 105-109 22.745 24.965 25.77 26.52 110-114 22.735 25.480000000000004 25.335 26.450000000000003 115-119 22.305 25.240000000000002 25.230000000000004 27.224999999999998 120-124 22.55 26.355 23.04 28.055000000000003 125-129 22.97 26.174999999999997 23.075000000000003 27.779999999999998 130-134 23.189999999999998 25.0 24.18 27.63 135-139 23.665 25.650000000000002 24.325 26.36 140-144 23.91 25.655 23.595 26.840000000000003 145-149 23.080000000000002 25.195 24.25 27.474999999999998 150-151 22.237499999999997 26.150000000000002 23.8375 27.775 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.5 3 0.5 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 1.0 22 1.0 23 0.0 24 1.0 25 3.0 26 3.0 27 3.5 28 5.0 29 7.0 30 7.0 31 8.0 32 11.5 33 13.0 34 11.5 35 20.5 36 61.0 37 113.5 38 104.5 39 87.0 40 115.0 41 126.5 42 111.5 43 110.5 44 108.0 45 112.0 46 128.0 47 123.5 48 119.0 49 130.0 50 159.0 51 161.5 52 163.5 53 185.5 54 220.0 55 276.0 56 273.0 57 215.0 58 179.0 59 147.0 60 101.5 61 71.0 62 51.0 63 38.0 64 29.5 65 17.0 66 16.5 67 15.0 68 8.5 69 3.5 70 2.5 71 6.0 72 3.5 73 0.5 74 1.0 75 4.5 76 4.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content warn #Base N-Count 1 5.8500000000000005 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 69.325 #Duplication Level Percentage of deduplicated Percentage of total 1 79.08402452217814 54.825 2 11.215290299314821 15.55 3 4.543815362423368 9.45 4 2.3800937612693835 6.6000000000000005 5 1.153984853948792 4.0 6 0.46880634691669676 1.95 7 0.3606202668589975 1.7500000000000002 8 0.2163721601153985 1.2 9 0.25243418680129825 1.575 >10 0.32455824017309776 3.1 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCATTAAT 30 0.75 No Hit CTCATAAGGACCACCATTGTATAACCATTCATCAACGGATGCAGCTTCCC 15 0.375 No Hit CCGGAACCCAAAGACTTTGATTTCTCATAAGGTGCCGGCGGAGTCCTATA 14 0.35000000000000003 No Hit GGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTACGTT 13 0.325 No Hit GCGGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTACG 12 0.3 No Hit CTGCGATGAAGGCGATAATAAATACAGAAGTTGCGGTCAATAAGGTAGGG 10 0.25 No Hit GTACAAGGCCCGGGAACGGATTCACCGCCGTATGGCTGACCGGCGATTAC 10 0.25 No Hit GTTGAAACTAGCATATTGGAAGATTAATCGGCCAAAATAACCATGAGCAG 10 0.25 No Hit CTTTCTTTTCCTCTGGCTACTAAGATGTTTCAGTTCGCCAGGTTGTCTCT 10 0.25 No Hit CGGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTACGT 9 0.22499999999999998 No Hit GTACAAAGGGCAGGGACGTAGTCAACGCGAGCTGATGACTCGCGCTTACT 9 0.22499999999999998 No Hit CTCCCATTTCGCTCGCCGCTACTACGGGAATCGCTTTTGCTTTCTTTTCC 9 0.22499999999999998 No Hit CATGCTCCACCGCTTGTGCGGGCCCCCGTCAATTCCTTTGAGTTTCATTC 9 0.22499999999999998 No Hit GTCGAGTTATCATGAATCATCGGATCAGCGAGCAAAGCCCGCGTCAGCCT 9 0.22499999999999998 No Hit CTCAGATACCGTCATTGTTTCTTCTCCGAGAAAAGAAGTTGACGACCCGT 9 0.22499999999999998 No Hit GTCGGTTCGGACCTCTGCTTAGTTTCATCCAAGCTTCATCCTGGTCATGG 9 0.22499999999999998 No Hit CGCAGAAGTAGGAATAATAGCACCAGAGATAATATTGTTTCCATAAAGTA 8 0.2 No Hit GGCGGTGTGTACAAAGGGCAGGGACGTAGTCAACGCGAGCTGATGACTCG 8 0.2 No Hit CCCGACTGTCCCTATTAATCATTACTCCGATCCCGAAGGCCAACACAATA 8 0.2 No Hit GCTTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCA 8 0.2 No Hit AGCGGAAAAACCTTATCCATTAATAGCGGGAACTTCAAGAGCAGCTAGAT 8 0.2 No Hit CACCATTGTATAACCATTCATCAACGGATGCAGCTTCCCAAATTGGGTAA 8 0.2 No Hit CACCGCTTGTGCGGGCCCCCGTCAATTCCTTTGAGTTTCATTCTTGCGAA 7 0.17500000000000002 No Hit CCTAGATGTCCAGTCAACTGCTGCGCCTCAACGCATTTCGGGGAGAACCA 7 0.17500000000000002 No Hit CACCTTTCCCTCACGGTACTACTTCGCTATCGGTCACCCAGGAGTATTTA 7 0.17500000000000002 No Hit CTCCACCGCTTGTGCGGGCCCCCGTCAATTCCTTTGAGTTTCATTCTTGC 7 0.17500000000000002 No Hit CACCTACAGACGCTTTACGCCCAATCATTCCGGATAACGCTTGCATCCTC 7 0.17500000000000002 No Hit CGAGAGTTGTTGAAACTAGCATATTGGAAGATTAATCGGCCAAAATAACC 7 0.17500000000000002 No Hit CATATAACAAGCTACACCAAGTAAGAAGTGTAGAACAATTAGCTCATAAG 7 0.17500000000000002 No Hit CTAGAATTACTACGGTTATCCGAGTAGCACGTACCATCAAACAAACTATA 7 0.17500000000000002 No Hit GGCAATTTCAAGCACTCTTTGACTCTCTTTTCAAAGTCCTTTTCATCTTT 7 0.17500000000000002 No Hit CCGCAGGCTCCACGCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTC 7 0.17500000000000002 No Hit CCCGAAGTTACGGGGCTATTTTGCCGAGTTCCTTAGAGAGAGTTGTCTCG 6 0.15 No Hit CCCTGACTCACCCTCCGTGGACGAACCTTGCGGAGGAAACCTTGGGTTTT 6 0.15 No Hit CGGCAATTTCAAGCACTCTTTGACTCTCTTTTCAAAGTCCTTTTCATCTT 6 0.15 No Hit GTCAATTCCTTTGAGTTTCATTCTTGCGAACGTACTCCCCAGGCGGGATA 6 0.15 No Hit CAACAACTTAAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGC 6 0.15 No Hit GGTGGGCTTACTACTTATATGCTTTCAGCAGTTATCCTCTCCGCACTTGG 6 0.15 No Hit GTTACGACTTCTCCTTCCTCTAAATGATAAGGTTCAATGGACTTCTCGCG 6 0.15 No Hit CTCAGTGTCAGTGTCGGCCCAGCAGAGTGCTTTCGCCGTTGGTGTTCTTT 6 0.15 No Hit GCCAAAATAACCATGAGCAGCCACAATATTATAAGTCTCTTCCTCTTGAC 6 0.15 No Hit GTTTACGGCTAGGACTACTGGGGTCTCTAATCCCATTTGCTCCCCTAGCT 6 0.15 No Hit CCTGTGTCGGTTTCGGGTACAGGTACCCTTTTGTTGAAGGTCGTTCGAGC 6 0.15 No Hit ACCACATGCTCCACCGCTTGTGCGGGCCCCCGTCAATTCCTTTGAGTTTC 6 0.15 No Hit CTCGCGAATACCATCAATATCTACTGGAGGGGCTGCGATGAAGGCGATAA 6 0.15 No Hit CTGGAGGGGCTGCGATGAAGGCGATAATAAATACAGAAGTTGCGGTCAAT 5 0.125 No Hit CCGCAGAAGTAGGAATAATAGCACCAGAGATAATATTGTTTCCATAAAGT 5 0.125 No Hit CAGATATTCCTAAAGGCATACCATCAGAGAAGCTTCCTTGACCAATAGGG 5 0.125 No Hit GGCGATAATAAATACAGAAGTTGCGGTCAATAAGGTAGGGATCATCAAAA 5 0.125 No Hit GTCGGTTTCGGGTACAGGTACCCTTTTGTTGAAGGTCGTTCGAGCTTTTC 5 0.125 No Hit CAAGTATTAATAACGCGACCTTGACTATCAACTACAGATTGGTTGAAATT 5 0.125 No Hit TGGAGGGGCTGCGATGAAGGCGATAATAAATACAGAAGTTGCGGTCAATA 5 0.125 No Hit GCCGACTTTCGTCTCTGCTCGACGGGTGAGTCTTGCAGTCAAGCTCCCTT 5 0.125 No Hit CCTCACGGTACTACTTCGCTATCGGTCACCCAGGAGTATTTAGCCTTGCA 5 0.125 No Hit CTTGTATTGCTCTCCCACAACCCCGTTTTCACGGTTTAGGCTGCTCCCAT 5 0.125 No Hit AGGAATAATAGCACCAGAGATAATATTGTTTCCATAAAGTAAAGAACCAG 5 0.125 No Hit GTCTTTCTGTCCAGGTGCAGGTAGTCCGCATCTTCACAGACATGTCTATT 5 0.125 No Hit CTCAAACTTCCGTCGCCTAAACGGCGATAGTCCCTCTAAGAAGCTAGCTG 5 0.125 No Hit TTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCATTAATA 5 0.125 No Hit CCTAATTCTCCGTCACCCGTCACCACCATGGTAGGCCCCTATCCTACCAT 5 0.125 No Hit CTCCCTTCTGCCTTTGCACTCGAGGACCAATGTCCGTCTGGCCCGAGGAA 5 0.125 No Hit CACGCTTTCACGGTTCGTATTCGTACTGGAAATCAGAATCAAACGAGCTT 5 0.125 No Hit CTTTCCCTCACGGTACTACTTCGCTATCGGTCACCCAGGAGTATTTAGCC 5 0.125 No Hit CCTCTAAGAAGCTAGCTGCGGAGGGATGGCTCCGCATAGCTAGTTAGCAG 5 0.125 No Hit GTCGGAACTTACCCGACAAGGAATTTCGCTACCTTAGGACCGTTATTGTT 5 0.125 No Hit CTACAGGCCAAGCAGCCAAGAAGAAGTGTAAAGAACGAGAGTTGTTGAAA 5 0.125 No Hit GTGTACAAAGGGCAGGGACGTAGTCAACGCGAGCTGATGACTCGCGCTTA 5 0.125 No Hit GTAACTTTTATCCGTTGAGCGACGGCCCTTCCACTCGGCACCGTCGGATC 5 0.125 No Hit CGCGGCTGCTGGCACCAGACTTGCCCTCCAATGGATCCTCGTTAAGGGAT 5 0.125 No Hit CTCTTGACCAAATTTGTAACCCTCATTAGCAGATTCATTTTCAGTAGTTT 5 0.125 No Hit CTCCACGCGGCATTGCTCCGTCAGGCTTTCGCCCATTGCGGAAAATTCCC 5 0.125 No Hit CTTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCAT 5 0.125 No Hit GTCTCGCGCCCCTAGGTATTCTCTACCTACCCACCTGTGTCGGTTTCGGG 5 0.125 No Hit GTCCAGTCAACTGCTGCGCCTCAACGCATTTCGGGGAGAACCAGCTAGCT 5 0.125 No Hit GGTAAATCAAGAAAACAGCAGTCGCAGCTGCAACAGGAGCTGAATATGCA 5 0.125 No Hit GTCGGGGCGGGCGGCGGGCGCAGGCGCCGCTTGCTAGCTTGGATTCTGAC 5 0.125 No Hit GTGGGCTTACTACTTATATGCTTTCAGCAGTTATCCTCTCCGCACTTGGC 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.025 0.0 0.0 0.0 0.0 68-69 0.075 0.0 0.0 0.0 0.0 70-71 0.075 0.0 0.0 0.0 0.0 72-73 0.1 0.0 0.0 0.0 0.0 74-75 0.1 0.0 0.0 0.0 0.0 76-77 0.1 0.0 0.0 0.0 0.0 78-79 0.125 0.0 0.0 0.0 0.0 80-81 0.16249999999999998 0.0 0.0 0.0 0.0 82-83 0.21250000000000002 0.0 0.0 0.0 0.0 84-85 0.275 0.0 0.0 0.0 0.0 86-87 0.375 0.0 0.0 0.0 0.0 88-89 0.4375 0.0 0.0 0.0 0.0 90-91 0.55 0.0 0.0 0.0 0.0 92-93 0.7125 0.0 0.0 0.0 0.0 94-95 0.8875 0.0 0.0 0.0 0.0 96-97 1.0125 0.0 0.0 0.0 0.0 98-99 1.225 0.0 0.0 0.0 0.0 100-101 1.4625 0.0 0.0 0.0 0.0 102-103 1.7625 0.0 0.0 0.0 0.0 104-105 2.05 0.0 0.0 0.0 0.0 106-107 2.35 0.0 0.0 0.0 0.0 108-109 2.6875 0.0 0.0 0.0 0.0 110-111 3.175 0.0 0.0 0.0 0.0 112-113 3.575 0.0 0.0 0.0 0.0 114-115 4.2375 0.0 0.0 0.0 0.0 116-117 4.6625 0.0 0.0 0.0 0.0 118-119 5.0625 0.0 0.0 0.0 0.0 120-121 5.5875 0.0 0.0 0.0 0.0 122-123 6.025 0.0 0.0 0.0 0.0 124-125 6.3375 0.0 0.0 0.0 0.0 126-127 6.825 0.0 0.0 0.0 0.0 128-129 7.387499999999999 0.0 0.0 0.0 0.0 130-131 7.9625 0.0 0.0 0.0 0.0 132-133 8.6125 0.0 0.0 0.0 0.0 134-135 9.3125 0.0 0.0 0.0 0.0 136-137 9.8625 0.0 0.0 0.0 0.0 138-139 10.5625 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGCTATT 10 0.0068378756 144.95 145 ATATGCA 10 0.0068378756 144.95 145 AAAAAAA 35 0.00354369 20.707142 135-139 CTTTTCA 50 0.0013329011 17.394 25-29 >>END_MODULE SRR6941601 read2 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR6941601_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 33.078 33.0 33.0 34.0 32.0 34.0 2 33.1465 34.0 33.0 34.0 33.0 34.0 3 33.1565 34.0 33.0 34.0 33.0 34.0 4 33.14375 34.0 33.0 34.0 33.0 34.0 5 33.1955 34.0 33.0 34.0 33.0 34.0 6 37.32325 38.0 38.0 38.0 37.0 38.0 7 37.258 38.0 38.0 38.0 37.0 38.0 8 37.2105 38.0 38.0 38.0 37.0 38.0 9 37.1235 38.0 38.0 38.0 37.0 38.0 10-14 36.96315 38.0 38.0 38.0 35.8 38.0 15-19 37.08710000000001 38.0 38.0 38.0 36.4 38.0 20-24 37.24855 38.0 38.0 38.0 37.0 38.0 25-29 37.18385 38.0 38.0 38.0 36.8 38.0 30-34 36.825599999999994 38.0 38.0 38.0 35.6 38.0 35-39 36.6997 38.0 38.0 38.0 34.8 38.0 40-44 37.1261 38.0 38.0 38.0 36.6 38.0 45-49 37.148 38.0 38.0 38.0 36.6 38.0 50-54 37.199650000000005 38.0 38.0 38.0 37.0 38.0 55-59 37.0514 38.0 38.0 38.0 36.2 38.0 60-64 37.08585 38.0 38.0 38.0 36.4 38.0 65-69 37.00605 38.0 38.0 38.0 36.2 38.0 70-74 36.70375 38.0 38.0 38.0 35.2 38.0 75-79 36.90390000000001 38.0 38.0 38.0 35.6 38.0 80-84 36.6981 38.0 38.0 38.0 35.2 38.0 85-89 36.46495 38.0 37.8 38.0 33.6 38.0 90-94 36.7641 38.0 38.0 38.0 35.0 38.0 95-99 36.63185 38.0 38.0 38.0 34.6 38.0 100-104 35.64785 38.0 36.8 38.0 30.4 38.0 105-109 35.953700000000005 38.0 36.8 38.0 32.0 38.0 110-114 36.50269999999999 38.0 38.0 38.0 34.2 38.0 115-119 36.41420000000001 38.0 38.0 38.0 34.0 38.0 120-124 36.035450000000004 38.0 37.6 38.0 32.6 38.0 125-129 35.8397 38.0 36.8 38.0 32.4 38.0 130-134 35.9165 38.0 37.2 38.0 33.0 38.0 135-139 32.9136 37.6 29.8 38.0 21.2 38.0 140-144 34.18145 37.6 33.2 38.0 27.0 38.0 145-149 35.05655 38.0 36.0 38.0 31.0 38.0 150-151 30.65725 35.5 29.5 38.0 15.5 38.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 3.0 4 1.0 5 0.0 6 0.0 7 1.0 8 0.0 9 3.0 10 2.0 11 1.0 12 1.0 13 2.0 14 3.0 15 1.0 16 2.0 17 3.0 18 0.0 19 0.0 20 5.0 21 1.0 22 6.0 23 3.0 24 14.0 25 13.0 26 13.0 27 17.0 28 22.0 29 33.0 30 48.0 31 53.0 32 75.0 33 98.0 34 146.0 35 288.0 36 730.0 37 2410.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 39.574999999999996 14.825 12.25 33.35 2 30.525000000000002 18.9 31.55 19.025 3 22.59194395796848 22.566925193895422 30.998248686514884 23.842882161621215 4 27.1703777833375 31.123342506880157 21.816362271703778 19.88991743807856 5 29.82236677508131 30.147610708031024 21.165874405804352 18.864148111083313 6 24.975 32.800000000000004 20.349999999999998 21.875 7 21.775 18.9 37.225 22.1 8 25.074999999999996 22.15 26.575 26.200000000000003 9 26.6 21.75 29.049999999999997 22.6 10-14 27.839999999999996 25.165 23.5 23.494999999999997 15-19 28.215 25.240000000000002 24.9 21.645 20-24 28.375 24.865000000000002 24.85 21.91 25-29 27.089999999999996 25.814999999999998 24.67 22.425 30-34 27.6 26.284999999999997 24.44 21.675 35-39 27.305 26.71 23.69 22.295 40-44 27.33 26.045 24.185000000000002 22.439999999999998 45-49 27.145000000000003 27.11 23.46 22.285 50-54 27.375 25.119999999999997 25.374999999999996 22.13 55-59 27.224999999999998 25.8 24.445 22.53 60-64 27.534999999999997 24.545 25.455 22.465 65-69 28.375 25.045 24.23 22.35 70-74 28.03 24.86 25.380000000000003 21.73 75-79 27.889999999999997 24.57 24.915000000000003 22.625 80-84 28.09 24.955 24.86 22.095000000000002 85-89 27.705000000000002 25.480000000000004 24.54 22.275 90-94 27.875 25.46 24.575 22.09 95-99 27.805000000000003 24.905 24.805 22.485 100-104 27.76 25.900000000000002 25.435000000000002 20.905 105-109 28.455000000000002 23.915 25.945 21.685 110-114 27.37 25.445 25.15 22.035 115-119 28.18 25.419999999999998 24.64 21.759999999999998 120-124 27.905 26.22 23.200000000000003 22.675 125-129 28.110000000000003 25.6 23.555 22.735 130-134 27.295 26.815 23.875 22.015 135-139 27.115000000000002 26.279999999999998 25.009999999999998 21.595 140-144 28.189999999999998 25.85 24.265 21.695 145-149 28.349999999999998 25.285000000000004 24.695 21.67 150-151 29.15 25.5625 23.4625 21.825 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.5 9 0.5 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.5 19 0.5 20 0.0 21 0.5 22 0.5 23 0.0 24 1.5 25 4.0 26 4.0 27 4.0 28 5.0 29 5.0 30 8.5 31 8.5 32 10.0 33 17.5 34 23.0 35 33.5 36 49.0 37 70.0 38 88.5 39 110.5 40 131.0 41 127.5 42 111.0 43 128.0 44 151.0 45 133.0 46 114.5 47 121.5 48 128.5 49 118.5 50 122.5 51 138.0 52 132.5 53 161.0 54 227.0 55 261.0 56 248.5 57 186.0 58 145.0 59 147.0 60 130.0 61 95.5 62 72.0 63 49.5 64 32.5 65 23.5 66 14.0 67 19.0 68 27.0 69 20.0 70 9.5 71 7.0 72 5.0 73 2.0 74 3.0 75 5.5 76 3.5 77 2.0 78 1.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.075 4 0.075 5 0.075 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 68.875 #Duplication Level Percentage of deduplicated Percentage of total 1 77.49546279491834 53.37499999999999 2 13.10344827586207 18.05 3 5.081669691470054 10.5 4 1.8874773139745917 5.2 5 1.0163339382940109 3.5000000000000004 6 0.2903811252268602 1.2 7 0.43557168784029043 2.1 8 0.18148820326678766 1.0 9 0.03629764065335753 0.22499999999999998 >10 0.47186932849364793 4.8500000000000005 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTTCGATCCTGGCTCAGGATGAACGCTGGCGGCATGCTTAACACATGCAA 30 0.75 No Hit CCTGCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGCAA 25 0.625 No Hit GGCTTGCGGTGGATACCTAGGCACCCAGAGACGAGGAAGGGCGTAGCAAG 22 0.5499999999999999 No Hit CTGCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGCAAG 15 0.375 No Hit ATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAA 13 0.325 No Hit CTTACCAGGTCCAGACATAGCAAGGATTGACAGACTGAGAGCTCTTTCTT 13 0.325 No Hit CCTGGCTCAGGATGAACGCTGGCGGCATGCTTAACACATGCAAGTCGAAC 12 0.3 No Hit AGTGACCAGGCCCGGGCGACTGTTTACCAAAAACACAGGTCTCCGCAAAG 12 0.3 No Hit CCTTACCAGGGCTTGACATGCCGCGAATCCTCTTGAAAGAGAGGGGTGCC 11 0.27499999999999997 No Hit GCTAACTCCAAAAACCCGTCCTCAGTTCGGATTGCAGGCTGCAACTCGCC 11 0.27499999999999997 No Hit TTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAA 10 0.25 No Hit GTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGATCTT 10 0.25 No Hit GTGACCAGGCCCGGGCGACTGTTTACCAAAAACACAGGTCTCCGCAAAGT 10 0.25 No Hit GGAAGCTTCTCTGATGGTATGCCTTTAGGAATATCTGGTACTTTCAACTT 9 0.22499999999999998 No Hit GGCTGATCTTCCCCAAGAGTCCACATCGACGGGAAGGTTTGGCACCTCGA 8 0.2 No Hit TGGGAACTTAGTTTCCGTTTGGGTATGCGCCCTTGGATTGCTGTTGCATA 8 0.2 No Hit GTCTGACATGCGTGCGAGTCGACGGGTTCTGAAACCTGGGATGCGCAAGG 8 0.2 No Hit GGAGTCTGACATGCGTGCGAGTCGACGGGTTCTGAAACCTGGGATGCGCA 8 0.2 No Hit GGATGATCAGCCACACTGGGACTGAGACACGGCCCAGACTCCTACGGGAG 8 0.2 No Hit GGAAACTACTGAAAATGAATCTGCTAATGAGGGTTACAAATTTGGTCAAG 7 0.17500000000000002 No Hit CCAAGATCTTACCATGACTGCAATTTTAGAGAGACGCGAAAGTACAAGCC 7 0.17500000000000002 No Hit TGCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGCAAGT 7 0.17500000000000002 No Hit CTTTACTTTATGGAAACAATATTATCTCTGGTGCTATTATTCCTACTTCT 7 0.17500000000000002 No Hit GCTTGTTATATGGGTCGTGAGTGGGAACTTAGTTTCCGTTTGGGTATGCG 7 0.17500000000000002 No Hit GTTTTGATGATCCCTACCTTATTGACCGCAACTTCTGTATTTATTATCGC 7 0.17500000000000002 No Hit CTTACCAGGGCTTGACATGCCGCGAATCCTCTTGAAAGAGAGGGGTGCCC 7 0.17500000000000002 No Hit CTGACACTGAGAGACGAAAGCTAGGGGAGCAAATGGGATTAGAGACCCCA 7 0.17500000000000002 No Hit ATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCA 7 0.17500000000000002 No Hit CAGGTCCAGACATAGCAAGGATTGACAGACTGAGAGCTCTTTCTTGATTC 7 0.17500000000000002 No Hit GTTTGATCAGGGAAACTACTGAAAATGAATCTGCTAATGAGGGTTACAAA 7 0.17500000000000002 No Hit CTTCCAATATGCTAGTTTCAACAACTCTCGTTCTTTACACTTCTTCTTGG 7 0.17500000000000002 No Hit ACCAGGTCCAGACATAGCAAGGATTGACAGACTGAGAGCTCTTTCTTGAT 6 0.15 No Hit TTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAG 6 0.15 No Hit GGCGGGACTACCCGCTGAGTTTAAGCATATAAATAAGCGGAGGAGAAGAA 6 0.15 No Hit TCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGA 6 0.15 No Hit GTTTGTTTGATGGTACGTGCTACTCGGATAACCGTAGTAATTCTAGAGCT 6 0.15 No Hit GCACAACATCCTTATGCATCCATTTCACATGTTAGGTGTAGCTGGTGTTT 6 0.15 No Hit GCTTAACACATGCAAGTCGAACGGGAAGTGGTGTTTCCAGTGGCGAACGG 6 0.15 No Hit GTCTGTGATGCCCTTAGATGTTCTGGGCCGCACGCGCGCTACACTGATGT 6 0.15 No Hit CAGGGCTTGACATGCCGCGAATCCTCTTGAAAGAGAGGGGTGCCCTCGGG 5 0.125 No Hit GGAATTTCCGGTGGAGCGGTGAAATGCATTGAGATCGGAAAGAACACCAA 5 0.125 No Hit GGTCGCTTCTGCAACTGGATAACTAGCACTGAAAATCGTCTTTACATCGG 5 0.125 No Hit TTCACATGTTAGGTGTAGCTGGTGTTTTCGGCGGTTCCCTATTCAGTGCT 5 0.125 No Hit GTGCTGAGCTCCCAAGCAGTGGGAGGGGAAAGTGATCTCTGACCGCGTGC 5 0.125 No Hit TATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAA 5 0.125 No Hit AGCGTCTGTAGGTGGCTTTTCAAGTCCGCCGTCAAATCCCAGGGCTCAAC 5 0.125 No Hit CAGGTCTGTGATGCCCTTAGATGTTCTGGGCCGCACGCGCGCTACACTGA 5 0.125 No Hit GCCGCGAATCCTCTTGAAAGAGAGGGGTGCCCTCGGGAACGCGGACACAG 5 0.125 No Hit GCTTGCGGTGGATACCTAGGCACCCAGAGACGAGGAAGGGCGTAGCAAGC 5 0.125 No Hit ATTTATTATCGCCTTCATCGCAGCCCCTCCAGTAGATATTGATGGTATTC 5 0.125 No Hit TGAAAATCGTCTTTACATCGGATGGTTCGGTGTTTTGATGATCCCTACCT 5 0.125 No Hit CTATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAA 5 0.125 No Hit CTTCTTTTCTCGGAGAAGAAACAATGACGGTATCTGAGGAATAAGCATCG 5 0.125 No Hit CTTGGATTTATGAAAGACGAACAACTGCGAAAGCATTTGCCAAGGATGTT 5 0.125 No Hit GTTGGGTTAAGTCTCGCAACGAGCGCAACCCTCGTGTTTAGTTGCCACTA 5 0.125 No Hit GCTGCGACTGCTGTTTTCTTGATTTACCCTATTGGTCAAGGAAGCTTCTC 5 0.125 No Hit CTTAGATGTTCTGGGCCGCACGCGCGCTACACTGATGTATTCAACGAGTA 5 0.125 No Hit GTTAGGTGTAGCTGGTGTTTTCGGCGGTTCCCTATTCAGTGCTATGCATG 5 0.125 No Hit TGCAATTTGAATAAACCAAGATCTTACCATGACTGCAATTTTAGAGAGAC 5 0.125 No Hit CCTCAGCCTGCTAACTAGCTATGCGGAGCCATCCCTCCGCAGCTAGCTTC 5 0.125 No Hit CGGCAATCGGATTGCACTTTTACCCAATTTGGGAAGCTGCATCCGTTGAT 5 0.125 No Hit CTCATAGGCAGTGGCTTGGTTAAGGGAACGGAACCCACCGGAGCCGTAGC 5 0.125 No Hit GGTACTTTCAACTTTATGATTGTATTCCAGGCAGAGCACAACATCCTTAT 5 0.125 No Hit GGCTTTTCAAGTCCGCCGTCAAATCCCAGGGCTCAACCCTGGACAGGCGG 5 0.125 No Hit TGAAAATAAGCATAGATCCGGAGATTCCCAAATAGGTCAACCTTTTAAAC 5 0.125 No Hit GCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGCAAGTA 5 0.125 No Hit GAACAGACCGCCGGTGTTAAGCCGGAGGAAGGAGAGGATGAGGCCAAGTC 5 0.125 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.025 0.0 0.0 0.0 0.0 68-69 0.075 0.0 0.0 0.0 0.0 70-71 0.075 0.0 0.0 0.0 0.0 72-73 0.1 0.0 0.0 0.0 0.0 74-75 0.1 0.0 0.0 0.0 0.0 76-77 0.1 0.0 0.0 0.0 0.0 78-79 0.125 0.0 0.0 0.0 0.0 80-81 0.16249999999999998 0.0 0.0 0.0 0.0 82-83 0.21250000000000002 0.0 0.0 0.0 0.0 84-85 0.275 0.0 0.0 0.0 0.0 86-87 0.375 0.0 0.0 0.0 0.0 88-89 0.4375 0.0 0.0 0.0 0.0 90-91 0.55 0.0 0.0 0.0 0.0 92-93 0.7125 0.0 0.0 0.0 0.0 94-95 0.875 0.0 0.0 0.0 0.0 96-97 0.9875 0.0 0.0 0.0 0.0 98-99 1.2 0.0 0.0 0.0 0.0 100-101 1.4375 0.0 0.0 0.0 0.0 102-103 1.6875 0.0 0.0 0.0 0.0 104-105 1.975 0.0 0.0 0.0 0.0 106-107 2.25 0.0 0.0 0.0 0.0 108-109 2.5875000000000004 0.0 0.0 0.0 0.0 110-111 3.075 0.0 0.0 0.0 0.0 112-113 3.4625 0.0 0.0 0.0 0.0 114-115 4.1 0.0 0.0 0.0 0.0 116-117 4.512499999999999 0.0 0.0 0.0 0.0 118-119 4.9375 0.0 0.0 0.0 0.0 120-121 5.4375 0.0 0.0 0.0 0.0 122-123 5.85 0.0 0.0 0.0 0.0 124-125 6.1125 0.0 0.0 0.0 0.0 126-127 6.5 0.0 0.0 0.0 0.0 128-129 6.925 0.0 0.0 0.0 0.0 130-131 7.3125 0.0 0.0 0.0 0.0 132-133 7.8375 0.0 0.0 0.0 0.0 134-135 8.35 0.0 0.0 0.0 0.0 136-137 8.7875 0.0 0.0 0.0 0.0 138-139 9.412500000000001 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATCCTGG 15 1.1411342E-4 145.0 6 TTTCTAG 10 0.006830828 145.0 5 TTATTTC 10 0.006830828 145.0 2 TTCGATC 15 1.1411342E-4 145.0 2 TAGTTAA 10 0.006830828 145.0 9 ATTATTT 10 0.006830828 145.0 1 CTGGCTC 15 1.1411342E-4 145.0 9 GATCCTG 15 1.1411342E-4 145.0 5 TATTTCT 10 0.006830828 145.0 3 CTAGTTA 10 0.006830828 145.0 8 TTCTAGT 10 0.006830828 145.0 6 ATACCCC 15 1.1411342E-4 145.0 145 TCCTGGC 15 1.1411342E-4 145.0 7 ATTTCTA 10 0.006830828 145.0 4 CCTGGCT 15 1.1411342E-4 145.0 8 TCGATCC 20 3.5877043E-4 108.75 3 GTTCGAT 20 3.5877043E-4 108.75 1 CGATCCT 20 3.5877043E-4 108.75 4 GAAACGG 20 0.00593511 29.0 130-134 TGCAAGT 20 0.00593511 29.0 45-49 >>END_MODULE Read 998348 spots for SRR6941601.sra Written 998348 spots for SRR6941601.sra Read 998348 spots for SRR6941601.sra Written 998348 spots for SRR6941601.sra Read 998348 spots for SRR6941601.sra Written 998348 spots for SRR6941601.sra Read 998348 spots for SRR6941601.sra Written 998348 spots for SRR6941601.sra Read 998348 spots for SRR6941601.sra Written 998348 spots for SRR6941601.sra Read 998348 spots for SRR6941601.sra Written 998348 spots for SRR6941601.sra Read 998348 spots for SRR6941601.sra Written 998348 spots for SRR6941601.sra Read 998348 spots for SRR6941601.sra Written 998348 spots for SRR6941601.sra Read 998348 spots for SRR6941601.sra Written 998348 spots for SRR6941601.sra Read 998348 spots for SRR6941601.sra Written 998348 spots for SRR6941601.sra Read 998348 spots for SRR6941601.sra Written 998348 spots for SRR6941601.sra Read 998348 spots for SRR6941601.sra Written 998348 spots for SRR6941601.sra Read 998348 spots for SRR6941601.sra Written 998348 spots for SRR6941601.sra Read 998348 spots for SRR6941601.sra Written 998348 spots for SRR6941601.sra Read 998358 spots for SRR6941601.sra Written 998358 spots for SRR6941601.sra Read 998348 spots for SRR6941601.sra Written 998348 spots for SRR6941601.sra Read 998348 spots for SRR6941601.sra Written 998348 spots for SRR6941601.sra Read 998348 spots for SRR6941601.sra Written 998348 spots for SRR6941601.sra Read 998348 spots for SRR6941601.sra Written 998348 spots for SRR6941601.sra Read 998348 spots for SRR6941601.sra Written 998348 spots for SRR6941601.sra SRR ids: ['SRR6941601.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_4xm0az6v SRR6941601.sra spots: 19966970 blocks: [[1, 998348], [998349, 1996696], [1996697, 2995044], [2995045, 3993392], [3993393, 4991740], [4991741, 5990088], [5990089, 6988436], [6988437, 7986784], [7986785, 8985132], [8985133, 9983480], [9983481, 10981828], [10981829, 11980176], [11980177, 12978524], [12978525, 13976872], [13976873, 14975220], [14975221, 15973568], [15973569, 16971916], [16971917, 17970264], [17970265, 18968612], [18968613, 19966970]] SRR6941601 file size 6744450 SRR6941601 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR6941601 SRR6941601_1.fastq SRR6941601_2.fastq Input file: SRR6941601_1.fastq Paired file: SRR6941601_2.fastq trimmed: SRR6941601-trimmed-pair1.fastq, SRR6941601-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Fri Dec 6 12:44:00 2024 >> started Fri Dec 6 12:44:25 2024 >> done (24.671s) 19966970 read pairs processed; of these: 23921 ( 0.12%) short read pairs filtered out after trimming by size control 17923 ( 0.09%) empty read pairs filtered out after trimming by size control 19925126 (99.79%) read pairs available; of these: 7864465 (39.47%) trimmed read pairs available after processing 12060661 (60.53%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 3 0.00% 19 3 0.00% 20 4 0.00% 21 6 0.00% 22 3 0.00% 23 2 0.00% 24 10 0.00% 25 8 0.00% 26 3 0.00% 27 8 0.00% 28 8 0.00% 29 11 0.00% 30 17 0.00% 31 15 0.00% 32 19 0.00% 33 17 0.00% 34 20 0.00% 35 20 0.00% 36 25 0.00% 37 22 0.00% 38 36 0.00% 39 31 0.00% 40 36 0.00% 41 45 0.00% 42 43 0.00% 43 51 0.00% 44 58 0.00% 45 63 0.00% 46 54 0.00% 47 69 0.00% 48 66 0.00% 49 108 0.00% 50 110 0.00% 51 118 0.00% 52 162 0.00% 53 155 0.00% 54 215 0.00% 55 204 0.00% 56 243 0.00% 57 260 0.00% 58 302 0.00% 59 307 0.00% 60 376 0.00% 61 468 0.00% 62 548 0.00% 63 615 0.00% 64 661 0.00% 65 861 0.00% 66 894 0.00% 67 915 0.00% 68 1138 0.01% 69 1234 0.01% 70 1440 0.01% 71 1605 0.01% 72 2062 0.01% 73 2169 0.01% 74 2410 0.01% 75 2627 0.01% 76 3101 0.02% 77 3402 0.02% 78 3695 0.02% 79 4536 0.02% 80 5201 0.03% 81 5596 0.03% 82 6419 0.03% 83 7155 0.04% 84 8395 0.04% 85 10494 0.05% 86 11397 0.06% 87 11539 0.06% 88 13131 0.07% 89 13460 0.07% 90 14279 0.07% 91 15466 0.08% 92 17908 0.09% 93 18865 0.09% 94 19489 0.10% 95 22674 0.11% 96 22929 0.12% 97 24848 0.12% 98 26298 0.13% 99 28312 0.14% 100 28433 0.14% 101 31418 0.16% 102 32050 0.16% 103 32639 0.16% 104 34609 0.17% 105 35623 0.18% 106 37388 0.19% 107 39537 0.20% 108 41278 0.21% 109 43618 0.22% 110 44271 0.22% 111 46142 0.23% 112 47634 0.24% 113 47262 0.24% 114 50130 0.25% 115 54475 0.27% 116 57380 0.29% 117 55387 0.28% 118 55567 0.28% 119 56550 0.28% 120 60944 0.31% 121 62132 0.31% 122 65400 0.33% 123 68714 0.34% 124 67746 0.34% 125 72506 0.36% 126 70732 0.35% 127 73352 0.37% 128 73884 0.37% 129 77376 0.39% 130 75257 0.38% 131 79058 0.40% 132 79102 0.40% 133 81630 0.41% 134 84651 0.42% 135 85065 0.43% 136 88421 0.44% 137 88168 0.44% 138 93293 0.47% 139 97688 0.49% 140 98157 0.49% 141 108010 0.54% 142 109826 0.55% 143 117519 0.59% 144 126777 0.64% 145 144017 0.72% 146 165061 0.83% 147 198629 1.00% 148 275459 1.38% 149 492413 2.47% 150 3270515 16.41% 151 12060661 60.53% 19925126 reads passed initial QC criterion=sequence-density sequence-density=2.24 sequence-density-rank=1 fanout-score=1.95 fanout-score-rank=27 prefix-density=2.20 prefix-fanout=2.0 sequence=TTCGCTATCGGTC criterion=fanout-score sequence-density=0.01 sequence-density-rank=37 fanout-score=305.34 fanout-score-rank=1 prefix-density=1.80 prefix-fanout=1.0 sequence=GTAGAACAAGATATTGGGTATTTCTCGCTTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCATTAATAGCGGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTACGTTCGTGCATTACTTCCATACCAAGATTAGCACGGTTGATGATATCAGCCCAAGTATTAATAACGCGACCTTGACTATCAACTACAGATTGGTTGAAATTGAATCCATTTAGGTTGAACGCCATAGTACTAATACCTAAAGCAGTGAACCAGATTCCTACTACAGGCCAAGCAGCCAAGAAGAAGTGTAAAGAACGAGAGTTGTTGAAACTAGCATATTGGAAGATTAATCGGCCAAAATAACCATGAGCAGCCACAATATTATAAGTCTCTTCCTCTTGACCAAATTTGTAACCCTCATTAGCAGATTCATTTTCAGTAGTTTCCCTGATCAAACTAGAGGTTACCAAGGAACCATGCATAGCACTGAATAGGGAACCG criterion=sequence-density sequence-density=4.21 sequence-density-rank=1 fanout-score=1.98 fanout-score-rank=26 prefix-density=4.17 prefix-fanout=2.0 sequence=GGTGGTGCATGGC criterion=fanout-score sequence-density=0.01 sequence-density-rank=30 fanout-score=103.80 fanout-score-rank=1 prefix-density=1.17 prefix-fanout=1.1 sequence=AGAAGGGGTGCCCCCTCACAAAAGGGGGTCGCAGTGACCAGGCCCGGGCGACTGTTTACCAAAAACACAGGTCTCCGCAAAGTCGTAAGACCATGTATGGGGGCTGACGCCTGCCCAGTGCCGGAAGGTCAAGGAAGTTGGTGAACTGATGACAGGGAAGCCGGCGACCGAAGCCCCGGTGAACGGCGGCCGTAAC Potential 3prime adapter identified. Now checking if in reference sequence /dee2/code/volunteer_pipeline.sh: line 905: -f: command not found /dee2/code/volunteer_pipeline.sh: line 906: -f: command not found Adapter seq not found in reference. Now shuffling file before clipping skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -t 20 -x TTCGCTATCGGTC -y GGTGGTGCATGGC -o SRR6941601 SRR6941601_1.fastq SRR6941601_2.fastq Input file: SRR6941601_1.fastq Paired file: SRR6941601_2.fastq trimmed: SRR6941601-trimmed-pair1.fastq, SRR6941601-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): TTCGCTATCGGTC -- paired 3' end adapter sequence (-y): GGTGGTGCATGGC -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Fri Dec 6 12:47:24 2024 >> started Fri Dec 6 12:47:35 2024 >> done (11.000s) 9962563 read pairs processed; of these: 3456 ( 0.03%) short read pairs filtered out after trimming by size control 1519 ( 0.02%) empty read pairs filtered out after trimming by size control 9957588 (99.95%) read pairs available; of these: 1487 ( 0.01%) trimmed read pairs available after processing 9956101 (99.99%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 19 1 0.00% 20 1 0.00% 21 3 0.00% 22 0 0.00% 23 1 0.00% 24 4 0.00% 25 4 0.00% 26 2 0.00% 27 3 0.00% 28 6 0.00% 29 7 0.00% 30 8 0.00% 31 7 0.00% 32 12 0.00% 33 11 0.00% 34 10 0.00% 35 7 0.00% 36 15 0.00% 37 14 0.00% 38 23 0.00% 39 10 0.00% 40 13 0.00% 41 17 0.00% 42 26 0.00% 43 25 0.00% 44 31 0.00% 45 29 0.00% 46 26 0.00% 47 35 0.00% 48 33 0.00% 49 54 0.00% 50 62 0.00% 51 55 0.00% 52 73 0.00% 53 73 0.00% 54 112 0.00% 55 107 0.00% 56 120 0.00% 57 114 0.00% 58 148 0.00% 59 156 0.00% 60 187 0.00% 61 250 0.00% 62 277 0.00% 63 305 0.00% 64 321 0.00% 65 431 0.00% 66 416 0.00% 67 440 0.00% 68 548 0.01% 69 600 0.01% 70 733 0.01% 71 795 0.01% 72 1056 0.01% 73 1098 0.01% 74 1213 0.01% 75 1359 0.01% 76 1591 0.02% 77 1699 0.02% 78 1888 0.02% 79 2263 0.02% 80 2562 0.03% 81 2819 0.03% 82 3185 0.03% 83 3647 0.04% 84 4207 0.04% 85 5120 0.05% 86 5711 0.06% 87 5784 0.06% 88 6545 0.07% 89 6656 0.07% 90 7207 0.07% 91 7809 0.08% 92 8916 0.09% 93 9355 0.09% 94 9777 0.10% 95 11477 0.12% 96 11494 0.12% 97 12280 0.12% 98 13155 0.13% 99 14096 0.14% 100 14213 0.14% 101 15885 0.16% 102 15956 0.16% 103 16304 0.16% 104 17356 0.17% 105 17882 0.18% 106 18666 0.19% 107 19729 0.20% 108 20710 0.21% 109 21676 0.22% 110 22059 0.22% 111 23007 0.23% 112 24138 0.24% 113 23723 0.24% 114 25142 0.25% 115 27197 0.27% 116 28682 0.29% 117 27794 0.28% 118 27643 0.28% 119 28102 0.28% 120 30400 0.31% 121 30938 0.31% 122 32584 0.33% 123 34485 0.35% 124 33972 0.34% 125 36410 0.37% 126 35509 0.36% 127 36483 0.37% 128 36938 0.37% 129 38672 0.39% 130 37835 0.38% 131 39519 0.40% 132 39354 0.40% 133 40818 0.41% 134 42372 0.43% 135 42794 0.43% 136 44307 0.44% 137 44265 0.44% 138 46626 0.47% 139 48774 0.49% 140 49253 0.49% 141 54219 0.54% 142 54821 0.55% 143 58415 0.59% 144 63480 0.64% 145 72237 0.73% 146 82288 0.83% 147 99630 1.00% 148 138041 1.39% 149 246423 2.47% 150 1633884 16.41% 151 6025248 60.51% criterion=sequence-density sequence-density=2.22 sequence-density-rank=1 fanout-score=1.96 fanout-score-rank=27 prefix-density=2.21 prefix-fanout=2.0 sequence=TTCGCTATCGGTC criterion=fanout-score sequence-density=0.01 sequence-density-rank=36 fanout-score=287.11 fanout-score-rank=1 prefix-density=1.78 prefix-fanout=1.0 sequence=GTAGAACAAGATATTGGGTATTTCTCGCTTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCATTAATAGCGGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTACGTTCGTGCATTACTTCCATACCAAGATTAGCACGGTTGATGATATCAGCCCAAGTATTAATAACGCGACCTTGACTATCAACTACAGATTGGTTGAAATTGAATCCATTTAGGTTGAACGCCATAGTACTAATACCTAAAGCAGTGAACCAGATTCCTACTACAGGCCAAGCAGCCAAGAAGAAGTGTAAAGAACGAGAGTTGTTGAAACTAGCATATTGGAAGATTAATCGGCCAAAATAACCATGAGCAGCCACAATATTATAAGTCTCTTCCTCTTGACCAAATTTGTAACCCTCATTAGCAGATTCATTTTCAGTAGTTTCCCTGATCAAACTAGAGGTTACCAAGGAACCATGCATAGCACTGAATAGGGAACCG criterion=sequence-density sequence-density=4.18 sequence-density-rank=1 fanout-score=1.98 fanout-score-rank=25 prefix-density=4.14 prefix-fanout=2.0 sequence=GGTGGTGCATGGC criterion=fanout-score sequence-density=0.01 sequence-density-rank=28 fanout-score=105.49 fanout-score-rank=1 prefix-density=1.16 prefix-fanout=1.1 sequence=AGAAGGGGTGCCCCCTCACAAAAGGGGGTCGCAGTGACCAGGCCCGGGCGACTGTTTACCAAAAACACAGGTCTCCGCAAAGTCGTAAGACCATGTATGGGGGCTGACGCCTGCCCAGTGCCGGAAGGTCAAGGAAGTTGGTGAACTGATGACAGGGAAGCCGGCGACCGAAGCCCCGGTGAACGGCGGCCGTAAC SRR6941601 testing PE reads STAR mapping to Ensembl genome Started job on | Dec 06 12:50:19 Started mapping on | Dec 06 12:50:20 Finished on | Dec 06 12:52:15 Mapping speed, Million of reads per hour | 623.59 Number of input reads | 19920151 Average input read length | 292 UNIQUE READS: Uniquely mapped reads number | 7931646 Uniquely mapped reads % | 39.82% Average mapped length | 295.31 Number of splices: Total | 953418 Number of splices: Annotated (sjdb) | 853240 Number of splices: GT/AG | 902655 Number of splices: GC/AG | 11595 Number of splices: AT/AC | 3805 Number of splices: Non-canonical | 35363 Mismatch rate per base, % | 0.25% Deletion rate per base | 0.02% Deletion average length | 1.87 Insertion rate per base | 0.01% Insertion average length | 2.31 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 6128161 % of reads mapped to multiple loci | 30.76% Number of reads mapped to too many loci | 816591 % of reads mapped to too many loci | 4.10% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 2.17% % of reads unmapped: other | 23.15% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 5866145 5866145 5866145 N_multimapping 6128161 6128161 6128161 N_noFeature 4957180 7771188 5024800 N_ambiguous 183411 1774 92663 UnstrandedReadsAssigned:2791055 PositiveStrandReadsAssigned:158684 NegativeStrandReadsAssigned:2814183 Dataset is classified negative stranded MeadianReadLen=151 20thPercentileLength=149 echo kmer=145 SRR6941601 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,972 [index] number of k-mers: 66,720,672 [index] number of equivalence classes: 111,837 [quant] running in paired-end mode [quant] will process pair 1: SRR6941601-trimmed-pair1.fastq SRR6941601-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 19,920,151 reads, 5,190,334 reads pseudoaligned [quant] estimated average fragment length: 207.987 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,028 rounds 52973 SRR6941601.ke.tsv 35125 SRR6941601.se.tsv 88098 total ==> SRR6941601.ke.tsv <== target_id length eff_length est_counts tpm PNS24245 936 729.38 14.5897 2.12191 PNS24247 1044 837.013 0 0 PNS24249 1928 1721.01 10.6773 0.658132 PNS24246 1044 837.013 0 0 PNS24248 1044 837.013 0 0 PNS24244 1471 1264.01 6.73297 0.565053 PNS24243 293 113.531 0 0 KQK14069 1603 1396.01 1020.79 77.568 KQK14071 474 274.206 17.7361 6.86145 ==> SRR6941601.se.tsv <== BRADI_1g14170v3 1272 BRADI_1g53295v3 32 BRADI_1g59795v3 39 BRADI_1g07683v3 0 BRADI_1g00485v3 0 BRADI_1g20270v3 23 BRADI_1g74790v3 1 BRADI_1g09890v3 0 BRADI_1g77505v3 24 BRADI_1g48960v3 0 SRR6941601 completed mapping pipeline successfully