Starting /dee2/code/volunteer_pipeline.sh SRR6941603 current disk space = 1551177768960 free memory = 1600815544 SRR6941603 SRAfilesize 5c0fdfa642def0989112e550a3df6bf9 SRR6941603.sra SRR6941603.sra file validated SRR6941603 is paired end SRR6941603 is conventional basespace SRR6941603 read1 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR6941603_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 51 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.6805 34.0 33.0 34.0 32.0 34.0 2 32.92875 34.0 33.0 34.0 32.0 34.0 3 33.09125 34.0 33.0 34.0 32.0 34.0 4 33.31175 34.0 33.0 34.0 32.0 34.0 5 33.332 34.0 33.0 34.0 33.0 34.0 6 37.16075 38.0 38.0 38.0 36.0 38.0 7 37.493 38.0 38.0 38.0 37.0 38.0 8 37.545 38.0 38.0 38.0 38.0 38.0 9 37.60275 38.0 38.0 38.0 38.0 38.0 10-14 37.60335 38.0 38.0 38.0 38.0 38.0 15-19 37.59535 38.0 38.0 38.0 38.0 38.0 20-24 37.533950000000004 38.0 38.0 38.0 38.0 38.0 25-29 37.5416 38.0 38.0 38.0 38.0 38.0 30-34 37.42965 38.0 38.0 38.0 37.8 38.0 35-39 37.46795 38.0 38.0 38.0 38.0 38.0 40-44 37.4983 38.0 38.0 38.0 37.8 38.0 45-49 37.41485 38.0 38.0 38.0 37.4 38.0 50-54 37.5246 38.0 38.0 38.0 38.0 38.0 55-59 37.4662 38.0 38.0 38.0 37.8 38.0 60-64 37.4242 38.0 38.0 38.0 37.0 38.0 65-69 37.3756 38.0 38.0 38.0 37.0 38.0 70-74 37.28215 38.0 38.0 38.0 37.0 38.0 75-79 37.31655 38.0 38.0 38.0 37.0 38.0 80-84 37.2855 38.0 38.0 38.0 36.8 38.0 85-89 37.189949999999996 38.0 38.0 38.0 36.6 38.0 90-94 37.195499999999996 38.0 38.0 38.0 36.2 38.0 95-99 37.15575 38.0 38.0 38.0 36.0 38.0 100-104 37.084 38.0 38.0 38.0 36.0 38.0 105-109 36.9784 38.0 38.0 38.0 35.4 38.0 110-114 36.58895 38.0 38.0 38.0 34.6 38.0 115-119 36.56325 38.0 38.0 38.0 34.6 38.0 120-124 36.754 38.0 38.0 38.0 35.0 38.0 125-129 36.668600000000005 38.0 38.0 38.0 34.4 38.0 130-134 36.481100000000005 38.0 38.0 38.0 34.0 38.0 135-139 36.3839 38.0 38.0 38.0 33.8 38.0 140-144 36.2904 38.0 38.0 38.0 33.8 38.0 145-149 36.01315 38.0 38.0 38.0 33.2 38.0 150-151 32.58 35.5 32.5 38.0 17.0 38.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 9 1.0 10 0.0 11 1.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 1.0 19 1.0 20 1.0 21 1.0 22 5.0 23 5.0 24 3.0 25 4.0 26 7.0 27 7.0 28 19.0 29 20.0 30 28.0 31 24.0 32 49.0 33 67.0 34 96.0 35 179.0 36 396.0 37 3085.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.92461255581823 11.741528762805359 7.2235355923299185 40.110323089046496 2 23.925 15.45 33.7 26.924999999999997 3 20.150000000000002 19.525000000000002 27.425 32.9 4 26.825 26.525 21.45 25.2 5 24.45 30.925000000000004 22.400000000000002 22.225 6 21.15 30.0 25.55 23.3 7 16.825000000000003 18.5 41.449999999999996 23.225 8 19.375 18.5 30.625000000000004 31.5 9 19.0 18.075 32.0 30.925000000000004 10-14 23.905 24.01 24.34 27.744999999999997 15-19 24.060000000000002 22.259999999999998 25.974999999999998 27.705000000000002 20-24 23.775 23.23 24.815 28.18 25-29 23.330000000000002 22.545 25.509999999999998 28.615000000000002 30-34 23.21 23.56 25.064999999999998 28.165000000000003 35-39 22.895 22.955000000000002 25.44 28.71 40-44 23.255 22.830000000000002 25.96 27.955000000000002 45-49 21.384276855371073 22.794558911782357 27.435487097419486 28.385677135427084 50-54 23.14 21.84 26.16 28.860000000000003 55-59 22.56 22.915 26.11 28.415000000000003 60-64 23.24 22.655 25.974999999999998 28.13 65-69 22.34 23.419999999999998 25.624999999999996 28.615000000000002 70-74 24.245 23.855 24.21 27.689999999999998 75-79 23.369999999999997 24.095 24.795 27.74 80-84 23.494999999999997 23.13 25.52 27.855 85-89 22.994999999999997 22.985 25.96 28.060000000000002 90-94 23.294999999999998 23.630000000000003 24.625 28.449999999999996 95-99 22.62 23.974999999999998 24.935 28.470000000000002 100-104 23.519111466880126 23.709225535321192 24.659795877526516 28.111867120272166 105-109 22.845 23.365 25.7 28.09 110-114 23.800904977375563 23.569632981397685 25.565610859728505 27.06385118149824 115-119 22.6882745471878 24.21353670162059 24.690181124880837 28.40800762631077 120-124 24.29 23.810000000000002 23.200000000000003 28.7 125-129 23.305 23.75 23.82 29.125 130-134 23.66 24.529999999999998 23.810000000000002 28.000000000000004 135-139 23.685000000000002 24.325 24.63 27.36 140-144 23.595 24.55 23.995 27.860000000000003 145-149 23.064999999999998 23.665 24.925 28.345 150-151 22.537499999999998 24.637500000000003 24.5625 28.262500000000003 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.5 20 0.5 21 0.5 22 1.0 23 1.0 24 1.5 25 2.5 26 2.5 27 3.5 28 4.5 29 7.0 30 7.0 31 6.0 32 10.0 33 14.5 34 13.5 35 15.5 36 45.0 37 72.0 38 58.5 39 63.5 40 90.0 41 100.0 42 90.5 43 77.0 44 75.0 45 96.5 46 112.0 47 96.5 48 91.0 49 107.5 50 141.0 51 162.5 52 173.0 53 212.0 54 251.0 55 301.0 56 321.0 57 272.5 58 232.5 59 190.5 60 143.0 61 91.5 62 52.0 63 42.5 64 30.5 65 24.0 66 19.0 67 10.5 68 12.0 69 8.0 70 3.5 71 7.0 72 9.5 73 8.5 74 5.0 75 3.0 76 3.5 77 2.0 78 0.5 79 0.5 80 0.5 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 4.825 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.02 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.06 105-109 0.0 110-114 0.5499999999999999 115-119 0.345 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 66.825 #Duplication Level Percentage of deduplicated Percentage of total 1 77.62813318368875 51.87500000000001 2 11.372989150766928 15.2 3 5.312383090160868 10.65 4 2.543958099513655 6.800000000000001 5 0.7856341189674524 2.625 6 0.8978675645342313 3.5999999999999996 7 0.411522633744856 1.925 8 0.3741114852225963 2.0 9 0.18705574261129815 1.125 >10 0.4863449307893753 4.2 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTACAAGGCCCGGGAACGGATTCACCGCCGTATGGCTGACCGGCGATTAC 22 0.5499999999999999 No Hit GTACAAAGGGCAGGGACGTAGTCAACGCGAGCTGATGACTCGCGCTTACT 15 0.375 No Hit GTCGGTTTCGGGTACAGGTACCCTTTTGTTGAAGGTCGTTCGAGCTTTTC 15 0.375 No Hit GTCAATTCCTTTGAGTTTCATTCTTGCGAACGTACTCCCCAGGCGGGATA 15 0.375 No Hit CTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCATTAAT 14 0.35000000000000003 No Hit GGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTACGTT 12 0.3 No Hit GTTGAAACTAGCATATTGGAAGATTAATCGGCCAAAATAACCATGAGCAG 12 0.3 No Hit GTTTACGGCTAGGACTACTGGGGTCTCTAATCCCATTTGCTCCCCTAGCT 12 0.3 No Hit GGCAATTTCAAGCACTCTTTGACTCTCTTTTCAAAGTCCTTTTCATCTTT 11 0.27499999999999997 No Hit ACATGCTCCACCGCTTGTGCGGGCCCCCGTCAATTCCTTTGAGTTTCATT 10 0.25 No Hit GCTCCACCGCTTGTGCGGGCCCCCGTCAATTCCTTTGAGTTTCATTCTTG 10 0.25 No Hit CATGCTCCACCGCTTGTGCGGGCCCCCGTCAATTCCTTTGAGTTTCATTC 10 0.25 No Hit CCGGAACCCAAAGACTTTGATTTCTCATAAGGTGCCGGCGGAGTCCTATA 10 0.25 No Hit CGTCAATTCCTTTGAGTTTCATTCTTGCGAACGTACTCCCCAGGCGGGAT 9 0.22499999999999998 No Hit GTCGCCCAGGGCATAAGGGGCATGATGACTTGGCCTCATCCTCTCCTTCC 9 0.22499999999999998 No Hit TTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCATTAATA 9 0.22499999999999998 No Hit GGGCTTACTACTTATATGCTTTCAGCAGTTATCCTCTCCGCACTTGGCTA 9 0.22499999999999998 No Hit CTCCACGCGGCATTGCTCCGTCAGGCTTTCGCCCATTGCGGAAAATTCCC 9 0.22499999999999998 No Hit CCTAGATGTCCAGTCAACTGCTGCGCCTCAACGCATTTCGGGGAGAACCA 8 0.2 No Hit CTCCACCGCTTGTGCGGGCCCCCGTCAATTCCTTTGAGTTTCATTCTTGC 8 0.2 No Hit GGCATGATGACTTGGCCTCATCCTCTCCTTCCTCCGGCTTAACACCGGCG 8 0.2 No Hit GGTCAATAAGGTAGGGATCATCAAAACACCGAACCATCCGATGTAAAGAC 8 0.2 No Hit ACAAAGGGCAGGGACGTAGTCAACGCGAGCTGATGACTCGCGCTTACTAG 8 0.2 No Hit CCCTACCGTACTCCAGCTTGGTAGTTTCCACCGCCTGTCCAGGGTTGAGC 8 0.2 No Hit GAAAAACCTTATCCATTAATAGCGGGAACTTCAAGAGCAGCTAGATCTAG 8 0.2 No Hit CTTTTCCTCTGGCTACTAAGATGTTTCAGTTCGCCAGGTTGTCTCTTGCC 8 0.2 No Hit CCTGTGTCGGTTTCGGGTACAGGTACCCTTTTGTTGAAGGTCGTTCGAGC 8 0.2 No Hit GGCTGTCTTTGCACCCCCACCTCCTTTATCACTGAGCGGTCATTTAGGGG 8 0.2 No Hit CACCGCTTGTGCGGGCCCCCGTCAATTCCTTTGAGTTTCATTCTTGCGAA 7 0.17500000000000002 No Hit CCTAGAGTAACTTTTATCCGTTGAGCGACGGCCCTTCCACTCGGCACCGT 7 0.17500000000000002 No Hit GCCAGCTCCTATAGTGTGACGGGCGGTGTGTACAAGGCCCGGGAACGGAT 7 0.17500000000000002 No Hit GGTGGGCTTACTACTTATATGCTTTCAGCAGTTATCCTCTCCGCACTTGG 7 0.17500000000000002 No Hit CTTAAACCTATAACCATCTTTCGGCTAACCTAGCCTCCTCCGTCCCTCCG 7 0.17500000000000002 No Hit GCGGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTACG 7 0.17500000000000002 No Hit CTCAGTGTCAGTGTCGGCCCAGCAGAGTGCTTTCGCCGTTGGTGTTCTTT 7 0.17500000000000002 No Hit CCGCATTAATGGGCGAACAGCCCAACCCTTGGAACCACCTACAGCTCCAG 7 0.17500000000000002 No Hit GCCTTTCGTGCGGGTCGGAACTTACCCGACAAGGAATTTCGCTACCTTAG 7 0.17500000000000002 No Hit CCCCACTGCTGCCTCCCGTAGGAGTCTGGGCCGTGTCTCAGTCCCAGTGT 7 0.17500000000000002 No Hit CTTTCTTTTCCTCTGGCTACTAAGATGTTTCAGTTCGCCAGGTTGTCTCT 7 0.17500000000000002 No Hit CGGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTACGT 6 0.15 No Hit GTTAGCTACAGCACTGCACGGGTCGAGTCGCACAGCACCTAGTATCCATC 6 0.15 No Hit CTTTATCACTGAGCGGTCATTTAGGGGCCTTAGCTGGTGATCCGGGCTGT 6 0.15 No Hit GTTGAAGACCAACAATTGCAATGATCTATCCCCATCACGATGAAATTTCC 6 0.15 No Hit CACCTACAGACGCTTTACGCCCAATCATTCCGGATAACGCTTGCATCCTC 6 0.15 No Hit GGGCGGTGTGTACAAGGCCCGGGAACGGATTCACCGCCGTATGGCTGACC 6 0.15 No Hit CGAGAGTTGTTGAAACTAGCATATTGGAAGATTAATCGGCCAAAATAACC 6 0.15 No Hit GTCAGTGTCGGCCCAGCAGAGTGCTTTCGCCGTTGGTGTTCTTTCCGATC 6 0.15 No Hit GTGTGTCGCCCAGGGCATAAGGGGCATGATGACTTGGCCTCATCCTCTCC 6 0.15 No Hit GTCAATTCCTTTAAGTTTCAGCCTTGCGACCATACTCCCCCCGGAACCCA 6 0.15 No Hit GCCGACTTTCGTCTCTGCTCGACGGGTGAGTCTTGCAGTCAAGCTCCCTT 6 0.15 No Hit CTTGTATTGCTCTCCCACAACCCCGTTTTCACGGTTTAGGCTGCTCCCAT 6 0.15 No Hit GGCATTGCTCCGTCAGGCTTTCGCCCATTGCGGAAAATTCCCCACTGCTG 6 0.15 No Hit CGGGAACGGATTCACCGCCGTATGGCTGACCGGCGATTACTAGCGATTCC 6 0.15 No Hit GTGTGTACAAGGCCCGGGAACGGATTCACCGCCGTATGGCTGACCGGCGA 6 0.15 No Hit CCTAATTCTCCGTCACCCGTCACCACCATGGTAGGCCCCTATCCTACCAT 6 0.15 No Hit GGAACGGATTCACCGCCGTATGGCTGACCGGCGATTACTAGCGATTCCTG 6 0.15 No Hit GTCGGTTCGGACCTCTGCTTAGTTTCATCCAAGCTTCATCCTGGTCATGG 6 0.15 No Hit GGCGGTGTGTACAAGGCCCGGGAACGGATTCACCGCCGTATGGCTGACCG 6 0.15 No Hit GGCTAACCTAGCCTCCTCCGTCCCTCCGTACCAACAAGGGGTAGTACAGG 6 0.15 No Hit GGTGTGTACAAGGCCCGGGAACGGATTCACCGCCGTATGGCTGACCGGCG 6 0.15 No Hit GTCCAGTCAACTGCTGCGCCTCAACGCATTTCGGGGAGAACCAGCTAGCT 6 0.15 No Hit GCTCCCTTCTGCCTTTGCACTCGAGGACCAATGTCCGTCTGGCCCGAGGA 6 0.15 No Hit CCGGGAACGGATTCACCGCCGTATGGCTGACCGGCGATTACTAGCGATTC 6 0.15 No Hit CCCGAAGTTACGGGGCTATTTTGCCGAGTTCCTTAGAGAGAGTTGTCTCG 5 0.125 No Hit CACGGTACTACTTCGCTATCGGTCACCCAGGAGTATTTAGCCTTGCAAGG 5 0.125 No Hit CCACAACCCCGTTTTCACGGTTTAGGCTGCTCCCATTTCGCTCGCCGCTA 5 0.125 No Hit CACGTGTGTCGCCCAGGGCATAAGGGGCATGATGACTTGGCCTCATCCTC 5 0.125 No Hit CAGATCGTTACGCCTTTCGTGCGGGTCGGAACTTACCCGACAAGGAATTT 5 0.125 No Hit GTCCGTACCAGTTCTGAGTCGACTGTTCAGCGCTCGGGGAAAGCCCCCGA 5 0.125 No Hit GCTAGGACTACTGGGGTCTCTAATCCCATTTGCTCCCCTAGCTTTCGTCT 5 0.125 No Hit GTGTACAAGGCCCGGGAACGGATTCACCGCCGTATGGCTGACCGGCGATT 5 0.125 No Hit CGGGCGGTGTGTACAAGGCCCGGGAACGGATTCACCGCCGTATGGCTGAC 5 0.125 No Hit CTCATCTTGGGGTGGGCTTACTACTTATATGCTTTCAGCAGTTATCCTCT 5 0.125 No Hit CATGAATCATCGGATCAGCGAGCAAAGCCCGCGTCAGCCTTTTATCTAAT 5 0.125 No Hit GTGCTCTGCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTCCTA 5 0.125 No Hit CCCACTGCTGCCTCCCGTAGGAGTCTGGGCCGTGTCTCAGTCCCAGTGTG 5 0.125 No Hit GTTTCTTCTCCGAGAAAAGAAGTTGACGACCCGTAGGCCTTCCACCTCCA 5 0.125 No Hit GTTGAATACATCAGTGTAGCGCGCGTGCGGCCCAGAACATCTAAGGGCAT 5 0.125 No Hit GTCAGGATTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGC 5 0.125 No Hit CTTTGTCCCGCCCATTGTAGCACGTGTGTCGCCCAGGGCATAAGGGGCAT 5 0.125 No Hit CACCAACTAGCTAATCAGACGCGAGCCCCTCCTTGGGCGGATTTCTCCTT 5 0.125 No Hit GTTCCAAACTCATAGTGGCAACTAAACACGAGGGTTGCGCTCGTTGCGAG 5 0.125 No Hit GCCCATTGTAGCACGTGTGTCGCCCAGGGCATAAGGGGCATGATGACTTG 5 0.125 No Hit CGCTACCTTAGGACCGTTATTGTTACGGCCGCCGTTCACCGGGGCTTCGG 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.0 0.0 0.0 0.0 0.0 70-71 0.05 0.0 0.0 0.0 0.0 72-73 0.05 0.0 0.0 0.0 0.0 74-75 0.075 0.0 0.0 0.0 0.0 76-77 0.0875 0.0 0.0 0.0 0.0 78-79 0.15 0.0 0.0 0.0 0.0 80-81 0.175 0.0 0.0 0.0 0.0 82-83 0.25 0.0 0.0 0.0 0.0 84-85 0.4 0.0 0.0 0.0 0.0 86-87 0.5 0.0 0.0 0.0 0.0 88-89 0.6000000000000001 0.0 0.0 0.0 0.0 90-91 0.7875 0.0 0.0 0.0 0.0 92-93 1.0375 0.0 0.0 0.0 0.0 94-95 1.2 0.0 0.0 0.0 0.0 96-97 1.525 0.0 0.0 0.0 0.0 98-99 1.7375 0.0 0.0 0.0 0.0 100-101 2.125 0.0 0.0 0.0 0.0 102-103 2.6 0.0 0.0 0.0 0.0 104-105 2.9625000000000004 0.0 0.0 0.0 0.0 106-107 3.375 0.0 0.0 0.0 0.0 108-109 3.625 0.0 0.0 0.0 0.0 110-111 4.074999999999999 0.0 0.0 0.0 0.0 112-113 4.6375 0.0 0.0 0.0 0.0 114-115 5.0375 0.0 0.0 0.0 0.0 116-117 5.3875 0.0 0.0 0.0 0.0 118-119 5.949999999999999 0.0 0.0 0.0 0.0 120-121 6.4875 0.0 0.0 0.0 0.0 122-123 7.0125 0.0 0.0 0.0 0.0 124-125 7.625 0.0 0.0 0.0 0.0 126-127 8.2875 0.0 0.0 0.0 0.0 128-129 8.9875 0.0 0.0 0.0 0.0 130-131 9.725000000000001 0.0 0.0 0.0 0.0 132-133 10.65 0.0 0.0 0.0 0.0 134-135 11.375 0.0 0.0 0.0 0.0 136-137 12.1375 0.0 0.0 0.0 0.0 138-139 12.850000000000001 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content pass >>END_MODULE SRR6941603 read2 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR6941603_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 51 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 33.16275 34.0 33.0 34.0 33.0 34.0 2 33.25375 34.0 33.0 34.0 33.0 34.0 3 33.2595 34.0 33.0 34.0 33.0 34.0 4 33.17175 34.0 33.0 34.0 33.0 34.0 5 33.2145 34.0 33.0 34.0 33.0 34.0 6 37.3685 38.0 38.0 38.0 38.0 38.0 7 37.445 38.0 38.0 38.0 38.0 38.0 8 37.402 38.0 38.0 38.0 38.0 38.0 9 37.3875 38.0 38.0 38.0 38.0 38.0 10-14 37.3439 38.0 38.0 38.0 37.6 38.0 15-19 37.29405 38.0 38.0 38.0 37.0 38.0 20-24 37.29540000000001 38.0 38.0 38.0 37.0 38.0 25-29 37.27065 38.0 38.0 38.0 37.0 38.0 30-34 37.29375 38.0 38.0 38.0 37.4 38.0 35-39 37.2976 38.0 38.0 38.0 37.0 38.0 40-44 37.290299999999995 38.0 38.0 38.0 37.0 38.0 45-49 37.259 38.0 38.0 38.0 37.0 38.0 50-54 37.24525 38.0 38.0 38.0 37.0 38.0 55-59 37.16015 38.0 38.0 38.0 37.0 38.0 60-64 37.07235 38.0 38.0 38.0 36.6 38.0 65-69 37.0135 38.0 38.0 38.0 36.6 38.0 70-74 37.0172 38.0 38.0 38.0 36.6 38.0 75-79 36.9985 38.0 38.0 38.0 36.0 38.0 80-84 36.98955 38.0 38.0 38.0 36.0 38.0 85-89 36.97765 38.0 38.0 38.0 36.0 38.0 90-94 36.885200000000005 38.0 38.0 38.0 35.6 38.0 95-99 36.779149999999994 38.0 38.0 38.0 35.0 38.0 100-104 36.60674999999999 38.0 38.0 38.0 34.8 38.0 105-109 36.46945 38.0 38.0 38.0 34.0 38.0 110-114 36.038149999999995 38.0 38.0 38.0 33.4 38.0 115-119 35.8154 38.0 37.6 38.0 32.2 38.0 120-124 35.8334 38.0 37.8 38.0 32.6 38.0 125-129 35.80800000000001 38.0 37.4 38.0 32.8 38.0 130-134 35.6765 38.0 37.0 38.0 32.0 38.0 135-139 35.481049999999996 38.0 36.4 38.0 31.0 38.0 140-144 35.00790000000001 38.0 36.0 38.0 30.6 38.0 145-149 33.66465 38.0 34.6 38.0 21.8 38.0 150-151 28.651 34.5 17.5 38.0 2.0 38.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4.0 3 5.0 4 2.0 5 1.0 6 0.0 7 0.0 8 0.0 9 2.0 10 2.0 11 0.0 12 0.0 13 0.0 14 3.0 15 0.0 16 2.0 17 1.0 18 4.0 19 6.0 20 6.0 21 3.0 22 4.0 23 4.0 24 3.0 25 10.0 26 10.0 27 27.0 28 25.0 29 35.0 30 35.0 31 53.0 32 55.0 33 82.0 34 145.0 35 196.0 36 507.0 37 2768.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 47.599999999999994 14.85 8.774999999999999 28.775000000000002 2 31.724999999999998 18.05 29.225 21.0 3 25.525 21.175 29.525000000000002 23.775 4 27.025 32.725 20.4 19.85 5 30.75 31.825 18.825 18.6 6 25.8 33.050000000000004 19.475 21.675 7 22.0 19.825 34.35 23.825 8 25.4 21.825 22.650000000000002 30.125 9 27.025 22.75 26.025 24.2 10-14 28.87 23.34 23.445 24.345 15-19 29.12 25.995 23.345 21.54 20-24 28.835 24.85 23.44 22.875 25-29 28.1 25.7 23.21 22.99 30-34 28.470000000000002 26.5 22.785 22.245 35-39 28.505000000000003 27.045 22.57 21.88 40-44 28.77 26.715 22.28 22.235 45-49 28.470000000000002 26.665 22.245 22.62 50-54 28.585 25.669999999999998 23.22 22.525000000000002 55-59 28.17 25.645 23.77 22.415 60-64 28.965000000000003 24.265 24.04 22.73 65-69 30.049999999999997 24.565 22.884999999999998 22.5 70-74 28.7 24.83 24.195 22.275 75-79 30.095 24.060000000000002 23.28 22.564999999999998 80-84 28.63 25.314999999999998 23.189999999999998 22.865 85-89 29.23 25.205 22.535 23.03 90-94 29.005 25.53 22.830000000000002 22.634999999999998 95-99 28.810000000000002 25.03 23.35 22.81 100-104 28.425 26.32 24.075 21.18 105-109 29.485897179435888 23.749749949989997 24.159831966393277 22.604520904180838 110-114 29.05790579057906 25.557555755575557 23.17231723172317 22.21222122212221 115-119 29.425 25.674999999999997 22.755 22.145 120-124 28.799999999999997 26.44 21.68 23.080000000000002 125-129 28.58 25.919999999999998 21.83 23.669999999999998 130-134 29.315 26.025 22.3 22.36 135-139 29.26 25.915 22.785 22.040000000000003 140-144 30.498049804980496 25.092509250925094 22.792279227922794 21.61716171617162 145-149 29.664832416208103 26.24312156078039 22.226113056528263 21.865932966483243 150-151 30.672006006757602 25.353522713052186 22.1499186584908 21.82455262169941 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.5 8 0.5 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 1.5 22 1.5 23 0.5 24 1.5 25 3.5 26 5.0 27 5.0 28 4.5 29 7.0 30 10.0 31 9.0 32 10.0 33 15.5 34 17.0 35 18.0 36 26.0 37 60.0 38 79.0 39 83.0 40 92.0 41 77.0 42 80.0 43 100.5 44 106.0 45 99.0 46 99.0 47 105.5 48 94.0 49 108.0 50 119.5 51 127.0 52 134.5 53 191.5 54 276.5 55 308.0 56 296.5 57 226.0 58 199.5 59 190.0 60 146.0 61 113.0 62 92.0 63 64.5 64 39.5 65 21.0 66 12.0 67 27.5 68 29.0 69 13.0 70 12.0 71 12.0 72 7.0 73 6.0 74 3.5 75 2.0 76 4.0 77 3.5 78 2.5 79 1.5 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.02 110-114 0.01 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.01 145-149 0.05 150-151 0.11249999999999999 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 67.025 #Duplication Level Percentage of deduplicated Percentage of total 1 76.72510257366653 51.425 2 13.465124953375607 18.05 3 4.923535994032078 9.9 4 2.051473330846699 5.5 5 1.0443864229765014 3.5000000000000004 6 0.5967922417008579 2.4 7 0.484893696381947 2.275 8 0.14919806042521447 0.8 9 0.07459903021260723 0.44999999999999996 >10 0.484893696381947 5.7 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTTCGATCCTGGCTCAGGATGAACGCTGGCGGCATGCTTAACACATGCAA 49 1.225 No Hit GGCTTGCGGTGGATACCTAGGCACCCAGAGACGAGGAAGGGCGTAGCAAG 24 0.6 No Hit CCTTACCAGGGCTTGACATGCCGCGAATCCTCTTGAAAGAGAGGGGTGCC 22 0.5499999999999999 No Hit GTCTGACATGCGTGCGAGTCGACGGGTTCTGAAACCTGGGATGCGCAAGG 19 0.475 No Hit CCTGGCTCAGGATGAACGCTGGCGGCATGCTTAACACATGCAAGTCGAAC 16 0.4 No Hit CTTACCAGGGCTTGACATGCCGCGAATCCTCTTGAAAGAGAGGGGTGCCC 15 0.375 No Hit ATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAA 14 0.35000000000000003 No Hit GCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGCAAGTA 14 0.35000000000000003 No Hit CCTGCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGCAA 12 0.3 No Hit GACCAGGCCCGGGCGACTGTTTACCAAAAACACAGGTCTCCGCAAAGTCG 12 0.3 No Hit CCTAGGCACCCAGAGACGAGGAAGGGCGTAGCAAGCGACGAAATGCTTCG 11 0.27499999999999997 No Hit AGTGACCAGGCCCGGGCGACTGTTTACCAAAAACACAGGTCTCCGCAAAG 10 0.25 No Hit CGCGAATCCTCTTGAAAGAGAGGGGTGCCCTCGGGAACGCGGACACAGGT 10 0.25 No Hit ACCAGGTCCAGACATAGCAAGGATTGACAGACTGAGAGCTCTTTCTTGAT 9 0.22499999999999998 No Hit GCTTGCGGTGGATACCTAGGCACCCAGAGACGAGGAAGGGCGTAGCAAGC 9 0.22499999999999998 No Hit GTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGATCTT 8 0.2 No Hit GGCGGGACTACCCGCTGAGTTTAAGCATATAAATAAGCGGAGGAGAAGAA 8 0.2 No Hit CTTACCAGGTCCAGACATAGCAAGGATTGACAGACTGAGAGCTCTTTCTT 8 0.2 No Hit GTTGGGTTAAGTCTCGCAACGAGCGCAACCCTCGTGTTTAGTTGCCACTA 8 0.2 No Hit CAGGGCTTGACATGCCGCGAATCCTCTTGAAAGAGAGGGGTGCCCTCGGG 7 0.17500000000000002 No Hit AGCACAACATCCTTATGCATCCATTTCACATGTTAGGTGTAGCTGGTGTT 7 0.17500000000000002 No Hit GGAATTTCCGGTGGAGCGGTGAAATGCATTGAGATCGGAAAGAACACCAA 7 0.17500000000000002 No Hit GGCTGATCTTCCCCAAGAGTCCACATCGACGGGAAGGTTTGGCACCTCGA 7 0.17500000000000002 No Hit CTTGAAAGAGAGGGGTGCCCTCGGGAACGCGGACACAGGTGGTGCATGGC 7 0.17500000000000002 No Hit CTTGCGGTGGATACCTAGGCACCCAGAGACGAGGAAGGGCGTAGCAAGCG 7 0.17500000000000002 No Hit GTTCTGGGCCGCACGCGCGCTACACTGATGTATTCAACGAGTATATAGCC 7 0.17500000000000002 No Hit GCCGCGAATCCTCTTGAAAGAGAGGGGTGCCCTCGGGAACGCGGACACAG 7 0.17500000000000002 No Hit GTGACCAGGCCCGGGCGACTGTTTACCAAAAACACAGGTCTCCGCAAAGT 7 0.17500000000000002 No Hit GCTAAATACTCCTGGGTGACCGATAGCGAAGTAGTACCGTGAGGGAAAGG 7 0.17500000000000002 No Hit GGAAGCTTCTCTGATGGTATGCCTTTAGGAATATCTGGTACTTTCAACTT 7 0.17500000000000002 No Hit ATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCA 7 0.17500000000000002 No Hit GTCTGTGATGCCCTTAGATGTTCTGGGCCGCACGCGCGCTACACTGATGT 7 0.17500000000000002 No Hit GTGCTGAGCTCCCAAGCAGTGGGAGGGGAAAGTGATCTCTGACCGCGTGC 6 0.15 No Hit CTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGAT 6 0.15 No Hit GAACCTTACCAGGGCTTGACATGCCGCGAATCCTCTTGAAAGAGAGGGGT 6 0.15 No Hit GAACGCTGGCGGCATGCTTAACACATGCAAGTCGAACGGGAAGTGGTGTT 6 0.15 No Hit CGGAATGATTGGGCGTAAAGCGTCTGTAGGTGGCTTTTCAAGTCCGCCGT 6 0.15 No Hit GGATTAGAGACCCCAGTAGTCCTAGCCGTAAACGATGGATACTAGGTGCT 6 0.15 No Hit CTTCTTTTCTCGGAGAAGAAACAATGACGGTATCTGAGGAATAAGCATCG 6 0.15 No Hit ACCAGGGCTTGACATGCCGCGAATCCTCTTGAAAGAGAGGGGTGCCCTCG 6 0.15 No Hit GACAAAGGGTCGCGATCTCGCGAGGGTGAGCTAACTCCAAAAACCCGTCC 6 0.15 No Hit GGGTGATCTATCCATGACCAGGATGAAGCTTGGATGAAACTAAGCAGAGG 6 0.15 No Hit TCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGA 6 0.15 No Hit GCTGCGACTGCTGTTTTCTTGATTTACCCTATTGGTCAAGGAAGCTTCTC 6 0.15 No Hit CTTAGATGTTCTGGGCCGCACGCGCGCTACACTGATGTATTCAACGAGTA 6 0.15 No Hit GGCAGAGGGAATTTCCGGTGGAGCGGTGAAATGCATTGAGATCGGAAAGA 6 0.15 No Hit AGAACCTTACCAGGGCTTGACATGCCGCGAATCCTCTTGAAAGAGAGGGG 6 0.15 No Hit CGGGAACGCGGACACAGGTGGTGCATGGCTGTCGTCAGCTCGTGCCGTAA 6 0.15 No Hit TTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAA 5 0.125 No Hit GGGGGCTCGAAGACGATCAGATACCGTCCTAGTCTCAACCATAAACGATG 5 0.125 No Hit GTACTTTCAACTTTATGATTGTATTCCAGGCAGAGCACAACATCCTTATG 5 0.125 No Hit GAGCGTTCCGCCTTAGAGGGAAGCAACCGCGAAAGCGGGGGTCGACGAAG 5 0.125 No Hit GGAAGGGCGTAGCAAGCGACGAAATGCTTCGGGGAGTTGAAAATAAGCAT 5 0.125 No Hit CTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCTAAATGGAT 5 0.125 No Hit GTTTAAGGACACAAGGTGACCCTGCTTTTTCAGGGTAAGAAGGGGTAGAG 5 0.125 No Hit GGAACAACAACTGGAAACGGTTGCTAATACCCCGTAGGCTGAGGAGCAAA 5 0.125 No Hit GGAAGGCCTACGGGTCGTCAACTTCTTTTCTCGGAGAAGAAACAATGACG 5 0.125 No Hit GCACTCTGCTGGGCCGACACTGACACTGAGAGACGAAAGCTAGGGGAGCA 5 0.125 No Hit GGTGAGTAACGCGTAAGAACCTGCCCTTGGGAGGGGAACAACAACTGGAA 5 0.125 No Hit GCGAATCCTCTTGAAAGAGAGGGGTGCCCTCGGGAACGCGGACACAGGTG 5 0.125 No Hit ATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCTAAATGG 5 0.125 No Hit CGGACATTGGTCCTCGAGTGCAAAGGCAGAAGGGAGCTTGACTGCAAGAC 5 0.125 No Hit GCCCAGACTCCTACGGGAGGCAGCAGTGGGGAATTTTCCGCAATGGGCGA 5 0.125 No Hit GCCTCCCAAAAGGTAACGGAGGCGTGCAAAGGTTTCCTCGGGCCAGACGG 5 0.125 No Hit ACCAGGCCCGGGCGACTGTTTACCAAAAACACAGGTCTCCGCAAAGTCGT 5 0.125 No Hit CATCAACCGTGCTAATCTTGGTATGGAAGTAATGCACGAACGTAATGCTC 5 0.125 No Hit CAGGATGAACGCTGGCGGCATGCTTAACACATGCAAGTCGAACGGGAAGT 5 0.125 No Hit ACCTTACCAGGGCTTGACATGCCGCGAATCCTCTTGAAAGAGAGGGGTGC 5 0.125 No Hit GCTGCATCCGTTGATGAATGGTTATACAATGGTGGTCCTTATGAGCTAAT 5 0.125 No Hit GGACAGTCTCAGGTAGACAGTTTCTATGGGGCGTAGGCCTCCCAAAAGGT 5 0.125 No Hit GTTAGGTGTAGCTGGTGTTTTCGGCGGTTCCCTATTCAGTGCTATGCATG 5 0.125 No Hit GCACAACATCCTTATGCATCCATTTCACATGTTAGGTGTAGCTGGTGTTT 5 0.125 No Hit GTCAAATCCCAGGGCTCAACCCTGGACAGGCGGTGGAAACTACCAAGCTG 5 0.125 No Hit GTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGATCTTACCATGAC 5 0.125 No Hit GTGGATACCTAGGCACCCAGAGACGAGGAAGGGCGTAGCAAGCGACGAAA 5 0.125 No Hit GCTAACTCCAAAAACCCGTCCTCAGTTCGGATTGCAGGCTGCAACTCGCC 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.0 0.0 0.0 0.0 0.0 70-71 0.05 0.0 0.0 0.0 0.0 72-73 0.05 0.0 0.0 0.0 0.0 74-75 0.075 0.0 0.0 0.0 0.0 76-77 0.0875 0.0 0.0 0.0 0.0 78-79 0.15 0.0 0.0 0.0 0.0 80-81 0.175 0.0 0.0 0.0 0.0 82-83 0.25 0.0 0.0 0.0 0.0 84-85 0.4 0.0 0.0 0.0 0.0 86-87 0.5 0.0 0.0 0.0 0.0 88-89 0.6000000000000001 0.0 0.0 0.0 0.0 90-91 0.7875 0.0 0.0 0.0 0.0 92-93 1.0625 0.0 0.0 0.0 0.0 94-95 1.25 0.0 0.0 0.0 0.0 96-97 1.5750000000000002 0.0 0.0 0.0 0.0 98-99 1.7875 0.0 0.0 0.0 0.0 100-101 2.175 0.0 0.0 0.0 0.0 102-103 2.65 0.0 0.0 0.0 0.0 104-105 3.0125 0.0 0.0 0.0 0.0 106-107 3.425 0.0 0.0 0.0 0.0 108-109 3.675 0.0 0.0 0.0 0.0 110-111 4.1375 0.0 0.0 0.0 0.0 112-113 4.7 0.0 0.0 0.0 0.0 114-115 5.1125 0.0 0.0 0.0 0.0 116-117 5.45 0.0 0.0 0.0 0.0 118-119 5.975 0.0 0.0 0.0 0.0 120-121 6.5 0.0 0.0 0.0 0.0 122-123 7.0375 0.0 0.0 0.0 0.0 124-125 7.6625 0.0 0.0 0.0 0.0 126-127 8.3375 0.0 0.0 0.0 0.0 128-129 9.0375 0.0 0.0 0.0 0.0 130-131 9.7625 0.0 0.0 0.0 0.0 132-133 10.6875 0.0 0.0 0.0 0.0 134-135 11.425 0.0 0.0 0.0 0.0 136-137 12.1875 0.0 0.0 0.0 0.0 138-139 12.899999999999999 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GACATGC 10 0.006830828 145.0 6 GATCCTG 10 0.006830828 145.0 5 TCCTGGC 10 0.006830828 145.0 7 >>END_MODULE Read 921991 spots for SRR6941603.sra Written 921991 spots for SRR6941603.sra Read 921991 spots for SRR6941603.sra Written 921991 spots for SRR6941603.sra Read 921991 spots for SRR6941603.sra Written 921991 spots for SRR6941603.sra Read 921991 spots for SRR6941603.sra Written 921991 spots for SRR6941603.sra Read 921991 spots for SRR6941603.sra Written 921991 spots for SRR6941603.sra Read 921991 spots for SRR6941603.sra Written 921991 spots for SRR6941603.sra Read 921991 spots for SRR6941603.sra Written 921991 spots for SRR6941603.sra Read 921992 spots for SRR6941603.sra Written 921992 spots for SRR6941603.sra Read 921991 spots for SRR6941603.sra Written 921991 spots for SRR6941603.sra Read 921991 spots for SRR6941603.sra Written 921991 spots for SRR6941603.sra Read 921991 spots for SRR6941603.sra Written 921991 spots for SRR6941603.sra Read 921991 spots for SRR6941603.sra Written 921991 spots for SRR6941603.sra Read 921991 spots for SRR6941603.sra Written 921991 spots for SRR6941603.sra Read 921991 spots for SRR6941603.sra Written 921991 spots for SRR6941603.sra Read 921991 spots for SRR6941603.sra Written 921991 spots for SRR6941603.sra Read 921991 spots for SRR6941603.sra Written 921991 spots for SRR6941603.sra Read 921991 spots for SRR6941603.sra Written 921991 spots for SRR6941603.sra Read 921991 spots for SRR6941603.sra Written 921991 spots for SRR6941603.sra Read 921991 spots for SRR6941603.sra Written 921991 spots for SRR6941603.sra Read 921991 spots for SRR6941603.sra Written 921991 spots for SRR6941603.sra SRR ids: ['SRR6941603.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_tsr5842y SRR6941603.sra spots: 18439821 blocks: [[1, 921991], [921992, 1843982], [1843983, 2765973], [2765974, 3687964], [3687965, 4609955], [4609956, 5531946], [5531947, 6453937], [6453938, 7375928], [7375929, 8297919], [8297920, 9219910], [9219911, 10141901], [10141902, 11063892], [11063893, 11985883], [11985884, 12907874], [12907875, 13829865], [13829866, 14751856], [14751857, 15673847], [15673848, 16595838], [16595839, 17517829], [17517830, 18439821]] SRR6941603 file size 6226949 SRR6941603 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR6941603 SRR6941603_1.fastq SRR6941603_2.fastq Input file: SRR6941603_1.fastq Paired file: SRR6941603_2.fastq trimmed: SRR6941603-trimmed-pair1.fastq, SRR6941603-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Fri Dec 6 12:51:21 2024 >> started Fri Dec 6 12:51:43 2024 >> done (21.247s) 18439821 read pairs processed; of these: 9922 ( 0.05%) short read pairs filtered out after trimming by size control 9888 ( 0.05%) empty read pairs filtered out after trimming by size control 18420011 (99.89%) read pairs available; of these: 8787025 (47.70%) trimmed read pairs available after processing 9632986 (52.30%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 2 0.00% 19 6 0.00% 20 4 0.00% 21 5 0.00% 22 4 0.00% 23 3 0.00% 24 10 0.00% 25 4 0.00% 26 6 0.00% 27 9 0.00% 28 13 0.00% 29 15 0.00% 30 15 0.00% 31 11 0.00% 32 11 0.00% 33 11 0.00% 34 11 0.00% 35 23 0.00% 36 16 0.00% 37 23 0.00% 38 26 0.00% 39 29 0.00% 40 27 0.00% 41 42 0.00% 42 53 0.00% 43 43 0.00% 44 51 0.00% 45 59 0.00% 46 66 0.00% 47 66 0.00% 48 59 0.00% 49 96 0.00% 50 125 0.00% 51 133 0.00% 52 188 0.00% 53 209 0.00% 54 227 0.00% 55 220 0.00% 56 260 0.00% 57 284 0.00% 58 357 0.00% 59 388 0.00% 60 436 0.00% 61 588 0.00% 62 660 0.00% 63 792 0.00% 64 887 0.00% 65 951 0.01% 66 1035 0.01% 67 1104 0.01% 68 1342 0.01% 69 1475 0.01% 70 1745 0.01% 71 1922 0.01% 72 2438 0.01% 73 2637 0.01% 74 2828 0.02% 75 3233 0.02% 76 3659 0.02% 77 4100 0.02% 78 4540 0.02% 79 5481 0.03% 80 6279 0.03% 81 6618 0.04% 82 7368 0.04% 83 9136 0.05% 84 9354 0.05% 85 11188 0.06% 86 11900 0.06% 87 12453 0.07% 88 14406 0.08% 89 14718 0.08% 90 15903 0.09% 91 17243 0.09% 92 19912 0.11% 93 20776 0.11% 94 21339 0.12% 95 24786 0.13% 96 24696 0.13% 97 26844 0.15% 98 26941 0.15% 99 29348 0.16% 100 30228 0.16% 101 34067 0.18% 102 32918 0.18% 103 33978 0.18% 104 36673 0.20% 105 37340 0.20% 106 37808 0.21% 107 40221 0.22% 108 43665 0.24% 109 44564 0.24% 110 44490 0.24% 111 46765 0.25% 112 48997 0.27% 113 48374 0.26% 114 50888 0.28% 115 54693 0.30% 116 55873 0.30% 117 52278 0.28% 118 54970 0.30% 119 53056 0.29% 120 57714 0.31% 121 60871 0.33% 122 62840 0.34% 123 67547 0.37% 124 66217 0.36% 125 71466 0.39% 126 69811 0.38% 127 71414 0.39% 128 68777 0.37% 129 72422 0.39% 130 69429 0.38% 131 73244 0.40% 132 75584 0.41% 133 77886 0.42% 134 80793 0.44% 135 81504 0.44% 136 87211 0.47% 137 87962 0.48% 138 94173 0.51% 139 96750 0.53% 140 98313 0.53% 141 112591 0.61% 142 116312 0.63% 143 126622 0.69% 144 143020 0.78% 145 169127 0.92% 146 196474 1.07% 147 245610 1.33% 148 357703 1.94% 149 648046 3.52% 150 3821475 20.75% 151 9632986 52.30% 18420011 reads passed initial QC criterion=sequence-density sequence-density=3.11 sequence-density-rank=1 fanout-score=1.95 fanout-score-rank=25 prefix-density=3.06 prefix-fanout=1.9 sequence=GTATTTAGCCTTG criterion=fanout-score sequence-density=0.01 sequence-density-rank=34 fanout-score=63.65 fanout-score-rank=1 prefix-density=0.15 prefix-fanout=2.5 sequence=CGACTTCACCCCAGTCGAAGACCCCACCGTGGTATGCGCCAATAAGACCACCAAAGGCCTTTGTGGCACTAGTGGTACACAGAAGTCATGGGTGATCATTGGTCCGATGCTTCGGGCGAAACCAATTCCCAGGGTGTGACGGGC criterion=sequence-density sequence-density=1.38 sequence-density-rank=1 fanout-score=5.82 fanout-score-rank=14 prefix-density=4.71 prefix-fanout=1.7 sequence=TGGTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGTTAACGAACGAGACCTCAGCCTGCTAACTAGCTATGCGGAGCCATCCCTCCGCAGCTAGCTTCTTAGAGGGACTATCGCCGTTTAGGCGACGGAAGTTTGAGGCAATAACAGGTCTGTGATGCCCTTAGATGTTCTGGGCCGCACGCGCGCTACACTGATGTATTCAACGAGTATATAGCCTTGGCCGACAGGCCCGGGTAATCTTGGGAAATTTCATCGTGATGGGGATAGATCATTGCAATTGTTGGTCTTCAACGAGGAATGCCTAGTAAGCGCGAGTCATCAGCTCGCGTTGACTACGTCCCTGCCCTTTGTACACACC criterion=fanout-score sequence-density=0.02 sequence-density-rank=35 fanout-score=84.74 fanout-score-rank=1 prefix-density=1.30 prefix-fanout=1.1 sequence=AGAAGGGGTGCCCCCTCACAAAAGGGGGTCGCAGTGACCAGGCCCGGGCGACTGTTTACCAAAAACACAGGTCTCCGCAAAGTCGTAAGACCATGTATGGGGGCTGACGCCTGCCCAGTGCCGGAAGGTCAAGGAAGTTGGTGAACTGATGACAGGGAAGCCGGCGACCGAAGCCCCGGTGAACGGCGGCCGTAAC Potential 3prime adapter identified. Now checking if in reference sequence /dee2/code/volunteer_pipeline.sh: line 905: -f: command not found /dee2/code/volunteer_pipeline.sh: line 906: -f: command not found Adapter seq not found in reference. Now shuffling file before clipping skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -t 20 -x GTATTTAGCCTTG -y TGGTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGTTAACGAACGAGACCTCAGCCTGCTAACTAGCTATGCGGAGCCATCCCTCCGCAGCTAGCTTCTTAGAGGGACTATCGCCGTTTAGGCGACGGAAGTTTGAGGCAATAACAGGTCTGTGATGCCCTTAGATGTTCTGGGCCGCACGCGCGCTACACTGATGTATTCAACGAGTATATAGCCTTGGCCGACAGGCCCGGGTAATCTTGGGAAATTTCATCGTGATGGGGATAGATCATTGCAATTGTTGGTCTTCAACGAGGAATGCCTAGTAAGCGCGAGTCATCAGCTCGCGTTGACTACGTCCCTGCCCTTTGTACACACC -o SRR6941603 SRR6941603_1.fastq SRR6941603_2.fastq Input file: SRR6941603_1.fastq Paired file: SRR6941603_2.fastq trimmed: SRR6941603-trimmed-pair1.fastq, SRR6941603-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): GTATTTAGCCTTG -- paired 3' end adapter sequence (-y): TGGTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGTTAACGAACGAGAC -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Fri Dec 6 12:53:41 2024 >> started Fri Dec 6 12:53:50 2024 >> done (8.981s) 6140004 read pairs processed; of these: 311 ( 0.01%) short read pairs filtered out after trimming by size control 1689 ( 0.03%) empty read pairs filtered out after trimming by size control 6138004 (99.97%) read pairs available; of these: 974 ( 0.02%) trimmed read pairs available after processing 6137030 (99.98%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 1 0.00% 19 4 0.00% 20 2 0.00% 21 0 0.00% 22 1 0.00% 23 1 0.00% 24 5 0.00% 25 3 0.00% 26 2 0.00% 27 4 0.00% 28 4 0.00% 29 4 0.00% 30 5 0.00% 31 8 0.00% 32 5 0.00% 33 4 0.00% 34 4 0.00% 35 9 0.00% 36 5 0.00% 37 10 0.00% 38 10 0.00% 39 12 0.00% 40 7 0.00% 41 14 0.00% 42 17 0.00% 43 7 0.00% 44 19 0.00% 45 20 0.00% 46 25 0.00% 47 25 0.00% 48 20 0.00% 49 28 0.00% 50 43 0.00% 51 36 0.00% 52 60 0.00% 53 81 0.00% 54 78 0.00% 55 86 0.00% 56 91 0.00% 57 94 0.00% 58 117 0.00% 59 140 0.00% 60 143 0.00% 61 188 0.00% 62 218 0.00% 63 262 0.00% 64 308 0.01% 65 324 0.01% 66 325 0.01% 67 358 0.01% 68 441 0.01% 69 476 0.01% 70 591 0.01% 71 628 0.01% 72 768 0.01% 73 936 0.02% 74 948 0.02% 75 1109 0.02% 76 1242 0.02% 77 1374 0.02% 78 1510 0.02% 79 1838 0.03% 80 2106 0.03% 81 2255 0.04% 82 2474 0.04% 83 2990 0.05% 84 3137 0.05% 85 3743 0.06% 86 3971 0.06% 87 4210 0.07% 88 4635 0.08% 89 4936 0.08% 90 5311 0.09% 91 5788 0.09% 92 6461 0.11% 93 6942 0.11% 94 6930 0.11% 95 8277 0.13% 96 8237 0.13% 97 8948 0.15% 98 8951 0.15% 99 9768 0.16% 100 10088 0.16% 101 11295 0.18% 102 10968 0.18% 103 11334 0.18% 104 12350 0.20% 105 12417 0.20% 106 12627 0.21% 107 13452 0.22% 108 14534 0.24% 109 14976 0.24% 110 14686 0.24% 111 15759 0.26% 112 16297 0.27% 113 16116 0.26% 114 16781 0.27% 115 18113 0.30% 116 18520 0.30% 117 17262 0.28% 118 18155 0.30% 119 17594 0.29% 120 19315 0.31% 121 20312 0.33% 122 21075 0.34% 123 22595 0.37% 124 21721 0.35% 125 23838 0.39% 126 23065 0.38% 127 23757 0.39% 128 22853 0.37% 129 24124 0.39% 130 23314 0.38% 131 24221 0.39% 132 25307 0.41% 133 26023 0.42% 134 26831 0.44% 135 27385 0.45% 136 29032 0.47% 137 29409 0.48% 138 31159 0.51% 139 32419 0.53% 140 32584 0.53% 141 37589 0.61% 142 38907 0.63% 143 42271 0.69% 144 47669 0.78% 145 56375 0.92% 146 65509 1.07% 147 81250 1.32% 148 118523 1.93% 149 215910 3.52% 150 1273945 20.76% 151 3211225 52.32% criterion=sequence-density sequence-density=3.12 sequence-density-rank=1 fanout-score=1.95 fanout-score-rank=24 prefix-density=3.08 prefix-fanout=2.0 sequence=GTATTTAGCCTTG criterion=fanout-score sequence-density=0.04 sequence-density-rank=29 fanout-score=31.03 fanout-score-rank=1 prefix-density=1.32 prefix-fanout=1.0 sequence=AGCACGTGTGTGGCCCAGCCCATAAGGGCCATGCGGACTTGACGTCATCCCCACCTTCCTCCAGTATCTCACTGGCAGTCCCTCGTGAGTGCGGCACGCACCTTTTTCTTTGTTTCGGAGCGGGGCGCGTACTATTACCACTACGTACCACACCACCGGGCGGCTGGCCTTCATGCCGAGTCTTCCTCCGCCGCCAACTCGACGTCGTCGTAACCAAGCCTAACCAAACCTCCATGCTCACTGGTACTTTGACGTCACTATAGGTAGGTCTCCGTGGGTCTTGAGTTCGGCAAGACGACTTCGGTTCACACGTGAAAGTGCTTCGAAAGGCACCGGCTCCCAATGGTGAAATTCTTGCTGAAAGCACAGCTACGTGCTGGCACTCAATCAAGTAGTAGCGCTGGCACGTCACTCGGCTCCTCGGCTCACTTCGGTGGCAATTTCTCCTTAGGCGCATGTCTCAGCAACACAAAACGAGGGTTTCGCTCGTTATAGGACTTGACCAAACATC criterion=sequence-density sequence-density=1.38 sequence-density-rank=1 fanout-score=5.82 fanout-score-rank=13 prefix-density=4.70 prefix-fanout=1.7 sequence=TGGTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGTTAACGAACGAGACCTCAGCCTGCTAACTAGCTATGCGGAGCCATCCCTCCGCAGCTAGCTTCTTAGAGGGACTATCGCCGTTTAGGCGACGGAAGTTTGAGGCAATAACAGGTCTGTGATGCCCTTAGATGTTCTGGGCCGCACGCGCGCTACACTGATGTATTCAACGAGTATATAGCCTTGGCCGACAGGCCCGGGTAATCTTGGGAAATTTCATCGTGATGGGGATAGATCATTGCAATTGTTGGTCTTCAACGAGGAATGCCTAGTAAGCGCGAGTCATCAGCTCGCGTTGACTACGTCCCTGCCCTTTGTACACACC criterion=fanout-score sequence-density=0.02 sequence-density-rank=35 fanout-score=87.99 fanout-score-rank=1 prefix-density=1.28 prefix-fanout=1.1 sequence=AGAAGGGGTGCCCCCTCACAAAAGGGGGTCGCAGTGACCAGGCCCGGGCGACTGTTTACCAAAAACACAGGTCTCCGCAAAGTCGTAAGACCATGTATGGGGGCTGACGCCTGCCCAGTGCCGGAAGGTCAAGGAAGTTGGTGAACTGATGACAGGGAAGCCGGCGACCGAAGCCCCGGTGAACGGCGGCCGTAAC SRR6941603 testing PE reads STAR mapping to Ensembl genome Started job on | Dec 06 12:54:35 Started mapping on | Dec 06 12:54:35 Finished on | Dec 06 12:56:09 Mapping speed, Million of reads per hour | 705.37 Number of input reads | 18418011 Average input read length | 290 UNIQUE READS: Uniquely mapped reads number | 7522857 Uniquely mapped reads % | 40.85% Average mapped length | 294.69 Number of splices: Total | 842335 Number of splices: Annotated (sjdb) | 744313 Number of splices: GT/AG | 789692 Number of splices: GC/AG | 10739 Number of splices: AT/AC | 2228 Number of splices: Non-canonical | 39676 Mismatch rate per base, % | 0.16% Deletion rate per base | 0.01% Deletion average length | 1.56 Insertion rate per base | 0.00% Insertion average length | 1.66 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 6395719 % of reads mapped to multiple loci | 34.73% Number of reads mapped to too many loci | 653583 % of reads mapped to too many loci | 3.55% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 2.90% % of reads unmapped: other | 17.98% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 4503834 4503834 4503834 N_multimapping 6395719 6395719 6395719 N_noFeature 5225270 7403971 5275390 N_ambiguous 148844 1378 81844 UnstrandedReadsAssigned:2148743 PositiveStrandReadsAssigned:117508 NegativeStrandReadsAssigned:2165623 Dataset is classified negative stranded MeadianReadLen=151 20thPercentileLength=148 echo kmer=143 SRR6941603 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,972 [index] number of k-mers: 66,720,672 [index] number of equivalence classes: 111,837 [quant] running in paired-end mode [quant] will process pair 1: SRR6941603-trimmed-pair1.fastq SRR6941603-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 18,418,011 reads, 4,555,508 reads pseudoaligned [quant] estimated average fragment length: 203.099 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 922 rounds 52973 SRR6941603.ke.tsv 35125 SRR6941603.se.tsv 88098 total ==> SRR6941603.ke.tsv <== target_id length eff_length est_counts tpm PNS24245 936 734.047 0 0 PNS24247 1044 841.901 3.54764 0.410947 PNS24249 1928 1725.9 4.72314 0.266884 PNS24246 1044 841.901 3.54764 0.410947 PNS24248 1044 841.901 3.54764 0.410947 PNS24244 1471 1268.9 4.63394 0.356148 PNS24243 293 117.172 0 0 KQK14069 1603 1400.9 973.973 67.8027 KQK14071 474 279.765 25.2992 8.81906 ==> SRR6941603.se.tsv <== BRADI_1g14170v3 1188 BRADI_1g53295v3 4 BRADI_1g59795v3 16 BRADI_1g07683v3 0 BRADI_1g00485v3 0 BRADI_1g20270v3 10 BRADI_1g74790v3 3 BRADI_1g09890v3 0 BRADI_1g77505v3 26 BRADI_1g48960v3 0 SRR6941603 completed mapping pipeline successfully