Starting /dee2/code/volunteer_pipeline.sh SRR6941604 current disk space = 1551084777472 free memory = 1604027252 SRR6941604 SRAfilesize 0e4c41551dcd66c133a4a78480371b0f SRR6941604.sra SRR6941604.sra file validated SRR6941604 is paired end SRR6941604 is conventional basespace SRR6941604 read1 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR6941604_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.45125 33.0 33.0 34.0 31.0 34.0 2 32.987 34.0 33.0 34.0 32.0 34.0 3 33.14975 34.0 33.0 34.0 32.0 34.0 4 33.31825 34.0 33.0 34.0 33.0 34.0 5 33.359 34.0 33.0 34.0 33.0 34.0 6 37.12725 38.0 38.0 38.0 36.0 38.0 7 37.49575 38.0 38.0 38.0 37.0 38.0 8 37.5695 38.0 38.0 38.0 38.0 38.0 9 37.585 38.0 38.0 38.0 38.0 38.0 10-14 37.66825 38.0 38.0 38.0 38.0 38.0 15-19 37.68945 38.0 38.0 38.0 38.0 38.0 20-24 37.66935 38.0 38.0 38.0 38.0 38.0 25-29 37.63205 38.0 38.0 38.0 38.0 38.0 30-34 37.5155 38.0 38.0 38.0 37.8 38.0 35-39 37.53940000000001 38.0 38.0 38.0 38.0 38.0 40-44 37.59265 38.0 38.0 38.0 38.0 38.0 45-49 37.612700000000004 38.0 38.0 38.0 38.0 38.0 50-54 37.553450000000005 38.0 38.0 38.0 38.0 38.0 55-59 37.5184 38.0 38.0 38.0 38.0 38.0 60-64 37.477250000000005 38.0 38.0 38.0 38.0 38.0 65-69 37.3617 38.0 38.0 38.0 37.2 38.0 70-74 37.339850000000006 38.0 38.0 38.0 37.0 38.0 75-79 37.366099999999996 38.0 38.0 38.0 37.0 38.0 80-84 37.4144 38.0 38.0 38.0 37.0 38.0 85-89 37.137 38.0 38.0 38.0 36.4 38.0 90-94 37.187200000000004 38.0 38.0 38.0 36.6 38.0 95-99 37.064949999999996 38.0 38.0 38.0 36.0 38.0 100-104 37.1563 38.0 38.0 38.0 36.0 38.0 105-109 36.92175 38.0 38.0 38.0 35.4 38.0 110-114 36.49335000000001 38.0 38.0 38.0 34.4 38.0 115-119 36.49675 38.0 38.0 38.0 34.2 38.0 120-124 36.5485 38.0 38.0 38.0 34.4 38.0 125-129 36.6386 38.0 38.0 38.0 34.8 38.0 130-134 36.427350000000004 38.0 38.0 38.0 34.2 38.0 135-139 36.1494 38.0 38.0 38.0 33.2 38.0 140-144 35.9581 38.0 37.6 38.0 32.8 38.0 145-149 35.4508 38.0 36.4 38.0 31.4 38.0 150-151 31.638125000000002 35.5 32.0 38.0 16.5 38.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 12 1.0 13 2.0 14 1.0 15 3.0 16 1.0 17 0.0 18 1.0 19 3.0 20 1.0 21 2.0 22 1.0 23 2.0 24 5.0 25 6.0 26 13.0 27 9.0 28 12.0 29 13.0 30 24.0 31 34.0 32 48.0 33 63.0 34 97.0 35 151.0 36 427.0 37 3080.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 40.54693662897713 11.254272942413884 7.283723376281882 40.91506705232711 2 23.63090772693173 14.603650912728183 33.50837709427357 28.257064266066518 3 19.55 20.1 24.6 35.75 4 26.275 26.724999999999998 22.275 24.725 5 23.75 31.3 23.375 21.575 6 19.7 32.4 25.3 22.6 7 15.425 22.675 40.2 21.7 8 19.6 19.775000000000002 31.324999999999996 29.299999999999997 9 18.475 19.925 32.75 28.849999999999998 10-14 23.385 25.66 23.915 27.04 15-19 22.625 23.885 26.185000000000002 27.305 20-24 22.41 25.174999999999997 25.885 26.529999999999998 25-29 22.54 23.580000000000002 26.245 27.634999999999998 30-34 22.745 24.959999999999997 25.72 26.575 35-39 22.09 24.64 27.065 26.205000000000002 40-44 22.996149807490372 24.08620431021551 25.891294564728234 27.026351317565876 45-49 21.37 24.6 27.24 26.790000000000003 50-54 22.451122556127807 24.09620481024051 26.09630481524076 27.35636781839092 55-59 22.365 24.265 26.305 27.065 60-64 22.189999999999998 24.015 26.875 26.919999999999998 65-69 22.439999999999998 25.224999999999998 25.16 27.175 70-74 23.255 25.224999999999998 24.57 26.950000000000003 75-79 22.575 24.959999999999997 25.515 26.950000000000003 80-84 23.580000000000002 24.36 25.169999999999998 26.889999999999997 85-89 22.49 24.060000000000002 25.955000000000002 27.495000000000005 90-94 21.725 25.4 25.165 27.71 95-99 22.725 25.22 24.55 27.505000000000003 100-104 22.916875062518756 25.067520256076826 24.9074722416725 27.108132439731918 105-109 22.95 24.685000000000002 25.69 26.674999999999997 110-114 23.156149517684888 25.180868167202576 25.291398713826368 26.371583601286176 115-119 22.431687139633993 25.76084231637002 24.722988217598395 27.08448232639759 120-124 22.469716688357195 26.153769146060668 23.290619681649815 28.08589448393233 125-129 23.49 25.474999999999998 23.615 27.42 130-134 23.849999999999998 25.645 23.27 27.235 135-139 23.23 25.3 24.795 26.674999999999997 140-144 23.615 25.974999999999998 23.655 26.755000000000003 145-149 22.705000000000002 24.925 24.905 27.465 150-151 21.525 26.0125 23.962500000000002 28.499999999999996 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 1.0 19 2.0 20 1.5 21 1.0 22 2.5 23 2.5 24 1.5 25 3.5 26 4.0 27 6.0 28 8.5 29 10.0 30 14.0 31 17.0 32 13.5 33 11.0 34 14.0 35 22.0 36 57.0 37 114.0 38 119.0 39 104.5 40 126.5 41 129.5 42 104.0 43 101.5 44 106.0 45 116.0 46 123.5 47 94.5 48 83.5 49 95.0 50 130.0 51 153.5 52 154.5 53 167.5 54 203.5 55 284.5 56 308.5 57 239.5 58 203.5 59 174.0 60 118.0 61 74.5 62 42.0 63 35.0 64 25.5 65 17.0 66 14.5 67 10.0 68 8.0 69 5.0 70 3.5 71 5.0 72 5.5 73 1.5 74 0.5 75 2.5 76 2.0 77 0.5 78 0.5 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 4.925 2 0.025 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.005 45-49 0.0 50-54 0.005 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.03 105-109 0.0 110-114 0.48 115-119 0.27499999999999997 120-124 0.11 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 67.325 #Duplication Level Percentage of deduplicated Percentage of total 1 78.94541403639064 53.15 2 11.139992573338285 15.0 3 4.455997029335314 9.0 4 1.708132194578537 4.6 5 1.4110657259561827 4.75 6 0.7426661715558857 3.0 7 0.6683995544002971 3.15 8 0.14853323431117713 0.8 9 0.14853323431117713 0.8999999999999999 >10 0.6312662458225028 5.65 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCATTAAT 23 0.575 No Hit GTACAAGGCCCGGGAACGGATTCACCGCCGTATGGCTGACCGGCGATTAC 23 0.575 No Hit GGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTACGTT 18 0.44999999999999996 No Hit GTCGGTTTCGGGTACAGGTACCCTTTTGTTGAAGGTCGTTCGAGCTTTTC 15 0.375 No Hit CTCATAAGGACCACCATTGTATAACCATTCATCAACGGATGCAGCTTCCC 15 0.375 No Hit GTACAAAGGGCAGGGACGTAGTCAACGCGAGCTGATGACTCGCGCTTACT 13 0.325 No Hit CACCGCTTGTGCGGGCCCCCGTCAATTCCTTTGAGTTTCATTCTTGCGAA 12 0.3 No Hit GTTTACGGCTAGGACTACTGGGGTCTCTAATCCCATTTGCTCCCCTAGCT 12 0.3 No Hit CCTAGATGTCCAGTCAACTGCTGCGCCTCAACGCATTTCGGGGAGAACCA 11 0.27499999999999997 No Hit CCTCACGGTACTACTTCGCTATCGGTCACCCAGGAGTATTTAGCCTTGCA 11 0.27499999999999997 No Hit TTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCATTAATA 11 0.27499999999999997 No Hit GCGGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTACG 11 0.27499999999999997 No Hit CTCAGATACCGTCATTGTTTCTTCTCCGAGAAAAGAAGTTGACGACCCGT 11 0.27499999999999997 No Hit CGGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTACGT 10 0.25 No Hit GTGTACAAGGCCCGGGAACGGATTCACCGCCGTATGGCTGACCGGCGATT 10 0.25 No Hit GTCGGTTCGGACCTCTGCTTAGTTTCATCCAAGCTTCATCCTGGTCATGG 10 0.25 No Hit GTTGAAACTAGCATATTGGAAGATTAATCGGCCAAAATAACCATGAGCAG 10 0.25 No Hit GGAAAAACCTTATCCATTAATAGCGGGAACTTCAAGAGCAGCTAGATCTA 9 0.22499999999999998 No Hit CGGCAATTTCAAGCACTCTTTGACTCTCTTTTCAAAGTCCTTTTCATCTT 9 0.22499999999999998 No Hit CCCACTGCTGCCTCCCGTAGGAGTCTGGGCCGTGTCTCAGTCCCAGTGTG 9 0.22499999999999998 No Hit CCCCACTGCTGCCTCCCGTAGGAGTCTGGGCCGTGTCTCAGTCCCAGTGT 9 0.22499999999999998 No Hit ACCGCTTGTGCGGGCCCCCGTCAATTCCTTTGAGTTTCATTCTTGCGAAC 8 0.2 No Hit GTCAATTCCTTTGAGTTTCATTCTTGCGAACGTACTCCCCAGGCGGGATA 8 0.2 No Hit GCCAAAATAACCATGAGCAGCCACAATATTATAAGTCTCTTCCTCTTGAC 8 0.2 No Hit GTCCAGTCAACTGCTGCGCCTCAACGCATTTCGGGGAGAACCAGCTAGCT 8 0.2 No Hit CTCCACCGCTTGTGCGGGCCCCCGTCAATTCCTTTGAGTTTCATTCTTGC 7 0.17500000000000002 No Hit CGCAGAAGTAGGAATAATAGCACCAGAGATAATATTGTTTCCATAAAGTA 7 0.17500000000000002 No Hit CCTCAGCCTACGGGGTATTAGCAACCGTTTCCAGTTGTTGTTCCCCTCCC 7 0.17500000000000002 No Hit GGCGCATACCCAAACGGAAACTAAGTTCCCACTCACGACCCATATAACAA 7 0.17500000000000002 No Hit CTCACGGTACTACTTCGCTATCGGTCACCCAGGAGTATTTAGCCTTGCAA 7 0.17500000000000002 No Hit GCTCCACCGCTTGTGCGGGCCCCCGTCAATTCCTTTGAGTTTCATTCTTG 7 0.17500000000000002 No Hit GCCAGCTCCTATAGTGTGACGGGCGGTGTGTACAAGGCCCGGGAACGGAT 7 0.17500000000000002 No Hit GGATGCATAAGGATGTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAA 7 0.17500000000000002 No Hit GTCGCCCAGGGCATAAGGGGCATGATGACTTGGCCTCATCCTCTCCTTCC 7 0.17500000000000002 No Hit GCTAGTTATCCAGTTGCAGAAGCGACCCCACAGGCTTGTACTTTCGCGTC 7 0.17500000000000002 No Hit GCTGCGATGAAGGCGATAATAAATACAGAAGTTGCGGTCAATAAGGTAGG 7 0.17500000000000002 No Hit GTGCTCTGCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTCCTA 7 0.17500000000000002 No Hit CCCTACCGTACTCCAGCTTGGTAGTTTCCACCGCCTGTCCAGGGTTGAGC 7 0.17500000000000002 No Hit CCGCATTAATGGGCGAACAGCCCAACCCTTGGAACCACCTACAGCTCCAG 7 0.17500000000000002 No Hit CTCCACGCGGCATTGCTCCGTCAGGCTTTCGCCCATTGCGGAAAATTCCC 7 0.17500000000000002 No Hit CTTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCAT 7 0.17500000000000002 No Hit CTTTTCCTCTGGCTACTAAGATGTTTCAGTTCGCCAGGTTGTCTCTTGCC 7 0.17500000000000002 No Hit CTTTCTTTTCCTCTGGCTACTAAGATGTTTCAGTTCGCCAGGTTGTCTCT 7 0.17500000000000002 No Hit CACCTGTGTCGGTTTCGGGTACAGGTACCCTTTTGTTGAAGGTCGTTCGA 6 0.15 No Hit GGCATGATGACTTGGCCTCATCCTCTCCTTCCTCCGGCTTAACACCGGCG 6 0.15 No Hit CCCACAACCCCGTTTTCACGGTTTAGGCTGCTCCCATTTCGCTCGCCGCT 6 0.15 No Hit CTCCCATTTCGCTCGCCGCTACTACGGGAATCGCTTTTGCTTTCTTTTCC 6 0.15 No Hit ACATGCTCCACCGCTTGTGCGGGCCCCCGTCAATTCCTTTGAGTTTCATT 6 0.15 No Hit CGGGAACGGATTCACCGCCGTATGGCTGACCGGCGATTACTAGCGATTCC 6 0.15 No Hit CCCTCATTAGCAGATTCATTTTCAGTAGTTTCCCTGATCAAACTAGAGGT 6 0.15 No Hit GTGTGTACAAGGCCCGGGAACGGATTCACCGCCGTATGGCTGACCGGCGA 6 0.15 No Hit GCTTGTATTGCTCTCCCACAACCCCGTTTTCACGGTTTAGGCTGCTCCCA 6 0.15 No Hit GTCCTCTCAATGCTCTAACGCCCACACCGGATATGGACCGAACTGTCTCA 6 0.15 No Hit GTTTCTTCTCCGAGAAAAGAAGTTGACGACCCGTAGGCCTTCCACCTCCA 6 0.15 No Hit GCATAAGGATGTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAAAGTA 6 0.15 No Hit CTCTTGACCAAATTTGTAACCCTCATTAGCAGATTCATTTTCAGTAGTTT 6 0.15 No Hit GGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTACGTTC 6 0.15 No Hit CCACACCGGATATGGACCGAACTGTCTCACGACGTTCTGAACCCAGCTCA 6 0.15 No Hit CACCATTGTATAACCATTCATCAACGGATGCAGCTTCCCAAATTGGGTAA 6 0.15 No Hit GCGGAAAAACCTTATCCATTAATAGCGGGAACTTCAAGAGCAGCTAGATC 6 0.15 No Hit CGCGACCTTGACTATCAACTACAGATTGGTTGAAATTGAATCCATTTAGG 6 0.15 No Hit ACCACATGCTCCACCGCTTGTGCGGGCCCCCGTCAATTCCTTTGAGTTTC 6 0.15 No Hit CTCGCGAATACCATCAATATCTACTGGAGGGGCTGCGATGAAGGCGATAA 6 0.15 No Hit CTCTAAGAAGCTAGCTGCGGAGGGATGGCTCCGCATAGCTAGTTAGCAGG 5 0.125 No Hit GGCCGACCTTGACCCCTGTTATTTTGGGGTCATATCTAGTATTCAGAGTT 5 0.125 No Hit CCCAATCATTCCGGATAACGCTTGCATCCTCTGTCTTACCGCGGCTGCTG 5 0.125 No Hit GCGGTCAATAAGGTAGGGATCATCAAAACACCGAACCATCCGATGTAAAG 5 0.125 No Hit CCACAACCCCGTTTTCACGGTTTAGGCTGCTCCCATTTCGCTCGCCGCTA 5 0.125 No Hit AAAAATTCTTATATGTTAGCGGAAAAACCTTATCCATTAATAGCGGGAAC 5 0.125 No Hit CACCTACAGACGCTTTACGCCCAATCATTCCGGATAACGCTTGCATCCTC 5 0.125 No Hit GTATTAATAACGCGACCTTGACTATCAACTACAGATTGGTTGAAATTGAA 5 0.125 No Hit GTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTC 5 0.125 No Hit CAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCATTAATAGCGGGAA 5 0.125 No Hit GCCGAAAACACCAGCTACACCTAACATGTGAAATGGATGCATAAGGATGT 5 0.125 No Hit GTCGCAGCTGCAACAGGAGCTGAATATGCAACAGCAATCCAAGGGCGCAT 5 0.125 No Hit GCTGAATATGCAACAGCAATCCAAGGGCGCATACCCAAACGGAAACTAAG 5 0.125 No Hit CCTTGGGCGGATTTCTCCTTTTGCTCCTCAGCCTACGGGGTATTAGCAAC 5 0.125 No Hit TTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCATT 5 0.125 No Hit ATAAGGACCACCATTGTATAACCATTCATCAACGGATGCAGCTTCCCAAA 5 0.125 No Hit CTTGTATTGCTCTCCCACAACCCCGTTTTCACGGTTTAGGCTGCTCCCAT 5 0.125 No Hit GTTCCCTTAACCAAGCCACTGCCTATGAGTCGCCGGCTCATTCTTCAACA 5 0.125 No Hit CCCAAGTATTAATAACGCGACCTTGACTATCAACTACAGATTGGTTGAAA 5 0.125 No Hit GGGAAACTTCGGAGGGAACCAGCTACTAGATGGTTCGATTAGTCTTTCGC 5 0.125 No Hit GTTACGACTTCTCCTTCCTCTAAATGATAAGGTTCAATGGACTTCTCGCG 5 0.125 No Hit GGTCAATAAGGTAGGGATCATCAAAACACCGAACCATCCGATGTAAAGAC 5 0.125 No Hit CGGAAAAACCTTATCCATTAATAGCGGGAACTTCAAGAGCAGCTAGATCT 5 0.125 No Hit ACAAAGGGCAGGGACGTAGTCAACGCGAGCTGATGACTCGCGCTTACTAG 5 0.125 No Hit CTTAAACCTATAACCATCTTTCGGCTAACCTAGCCTCCTCCGTCCCTCCG 5 0.125 No Hit CTTTCCCTCACGGTACTACTTCGCTATCGGTCACCCAGGAGTATTTAGCC 5 0.125 No Hit GGGCTTACTACTTATATGCTTTCAGCAGTTATCCTCTCCGCACTTGGCTA 5 0.125 No Hit AGCGGAAAAACCTTATCCATTAATAGCGGGAACTTCAAGAGCAGCTAGAT 5 0.125 No Hit AGCACGTGTGTCGCCCAGGGCATAAGGGGCATGATGACTTGGCCTCATCC 5 0.125 No Hit GGGTGGGCTTACTACTTATATGCTTTCAGCAGTTATCCTCTCCGCACTTG 5 0.125 No Hit CTAGTATTCAGAGTTTGCCTCGATTTGGTACCGCTCGCGCAGCCCGCACC 5 0.125 No Hit GGCGGTGTGTACAAGGCCCGGGAACGGATTCACCGCCGTATGGCTGACCG 5 0.125 No Hit TTCACCTTTCCCTCACGGTACTACTTCGCTATCGGTCACCCAGGAGTATT 5 0.125 No Hit GGCAATTTCAAGCACTCTTTGACTCTCTTTTCAAAGTCCTTTTCATCTTT 5 0.125 No Hit GCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTCCTAAAGGCAT 5 0.125 No Hit CCGGAACCCAAAGACTTTGATTTCTCATAAGGTGCCGGCGGAGTCCTATA 5 0.125 No Hit GTTGTCTCTTGCCTGCTCATGGATTCAGCAGGCAGTTTAAAAGGTTGACC 5 0.125 No Hit GTGGGCTTACTACTTATATGCTTTCAGCAGTTATCCTCTCCGCACTTGGC 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.0125 0.0 0.0 0.0 0.0 70-71 0.05 0.0 0.0 0.0 0.0 72-73 0.05 0.0 0.0 0.0 0.0 74-75 0.07500000000000001 0.0 0.0 0.0 0.0 76-77 0.125 0.0 0.0 0.0 0.0 78-79 0.125 0.0 0.0 0.0 0.0 80-81 0.125 0.0 0.0 0.0 0.0 82-83 0.16249999999999998 0.0 0.0 0.0 0.0 84-85 0.25 0.0 0.0 0.0 0.0 86-87 0.3625 0.0 0.0 0.0 0.0 88-89 0.475 0.0 0.0 0.0 0.0 90-91 0.625 0.0 0.0 0.0 0.0 92-93 0.8125 0.0 0.0 0.0 0.0 94-95 1.075 0.0 0.0 0.0 0.0 96-97 1.3 0.0 0.0 0.0 0.0 98-99 1.55 0.0 0.0 0.0 0.0 100-101 1.9125 0.0 0.0 0.0 0.0 102-103 2.2625 0.0 0.0 0.0 0.0 104-105 2.5375 0.0 0.0 0.0 0.0 106-107 2.85 0.0 0.0 0.0 0.0 108-109 3.175 0.0 0.0 0.0 0.0 110-111 3.5875 0.0 0.0 0.0 0.0 112-113 4.0375 0.0 0.0 0.0 0.0 114-115 4.4625 0.0 0.0 0.0 0.0 116-117 4.9125 0.0 0.0 0.0 0.0 118-119 5.5375 0.0 0.0 0.0 0.0 120-121 6.375 0.0 0.0 0.0 0.0 122-123 6.887499999999999 0.0 0.0 0.0 0.0 124-125 7.675000000000001 0.0 0.0 0.0 0.0 126-127 8.2375 0.0 0.0 0.0 0.0 128-129 8.975 0.0 0.0 0.0 0.0 130-131 9.3875 0.0 0.0 0.0 0.0 132-133 10.225 0.0 0.0 0.0 0.0 134-135 10.9625 0.0 0.0 0.0 0.0 136-137 11.5125 0.0 0.0 0.0 0.0 138-139 12.3625 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTCGGAT 10 0.0059484434 151.76317 1 TTTCTTC 40 0.00574973 54.065628 2 CCAGTCA 35 0.003655537 20.596428 140-144 >>END_MODULE SRR6941604 read2 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR6941604_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 50 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 33.198 34.0 33.0 34.0 33.0 34.0 2 33.321 34.0 33.0 34.0 33.0 34.0 3 33.3805 34.0 33.0 34.0 33.0 34.0 4 33.3215 34.0 33.0 34.0 33.0 34.0 5 33.30325 34.0 33.0 34.0 33.0 34.0 6 37.4905 38.0 38.0 38.0 38.0 38.0 7 37.5195 38.0 38.0 38.0 38.0 38.0 8 37.41175 38.0 38.0 38.0 38.0 38.0 9 37.46675 38.0 38.0 38.0 38.0 38.0 10-14 37.4499 38.0 38.0 38.0 38.0 38.0 15-19 37.4251 38.0 38.0 38.0 38.0 38.0 20-24 37.4125 38.0 38.0 38.0 37.8 38.0 25-29 37.364549999999994 38.0 38.0 38.0 37.6 38.0 30-34 37.41635 38.0 38.0 38.0 38.0 38.0 35-39 37.40945000000001 38.0 38.0 38.0 38.0 38.0 40-44 37.378699999999995 38.0 38.0 38.0 38.0 38.0 45-49 37.3487 38.0 38.0 38.0 38.0 38.0 50-54 37.348749999999995 38.0 38.0 38.0 37.4 38.0 55-59 37.304649999999995 38.0 38.0 38.0 37.0 38.0 60-64 37.263600000000004 38.0 38.0 38.0 37.0 38.0 65-69 37.187599999999996 38.0 38.0 38.0 37.0 38.0 70-74 37.246449999999996 38.0 38.0 38.0 37.0 38.0 75-79 37.22474999999999 38.0 38.0 38.0 37.0 38.0 80-84 37.1201 38.0 38.0 38.0 36.8 38.0 85-89 37.13635000000001 38.0 38.0 38.0 36.8 38.0 90-94 37.0451 38.0 38.0 38.0 36.0 38.0 95-99 36.929449999999996 38.0 38.0 38.0 35.8 38.0 100-104 36.713699999999996 38.0 38.0 38.0 35.0 38.0 105-109 36.54615 38.0 38.0 38.0 34.6 38.0 110-114 36.1684 38.0 38.0 38.0 33.8 38.0 115-119 36.01535 38.0 37.8 38.0 33.0 38.0 120-124 35.9554 38.0 37.6 38.0 32.8 38.0 125-129 36.02405 38.0 38.0 38.0 33.2 38.0 130-134 36.07835 38.0 38.0 38.0 33.4 38.0 135-139 35.754949999999994 38.0 37.2 38.0 31.4 38.0 140-144 35.22435 38.0 36.0 38.0 31.0 38.0 145-149 34.1157 38.0 34.4 38.0 25.6 38.0 150-151 29.427125000000004 35.5 27.0 38.0 7.5 38.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4.0 3 3.0 4 1.0 5 0.0 6 0.0 7 2.0 8 1.0 9 0.0 10 0.0 11 0.0 12 0.0 13 1.0 14 0.0 15 3.0 16 2.0 17 1.0 18 1.0 19 1.0 20 6.0 21 4.0 22 6.0 23 4.0 24 8.0 25 9.0 26 14.0 27 11.0 28 14.0 29 19.0 30 40.0 31 47.0 32 45.0 33 78.0 34 125.0 35 182.0 36 501.0 37 2867.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 44.675 16.725 11.4 27.200000000000003 2 32.75 17.4 30.725 19.125 3 22.6 22.7 31.275 23.425 4 26.674999999999997 30.675 23.775 18.875 5 30.049999999999997 32.85 19.1 18.0 6 25.025 34.25 20.9 19.825 7 21.575 19.875 36.65 21.9 8 24.525 21.95 25.124999999999996 28.4 9 25.775 22.325 27.55 24.349999999999998 10-14 28.549999999999997 23.990000000000002 23.75 23.71 15-19 27.975 25.679999999999996 24.515 21.83 20-24 28.389999999999997 24.545 24.605 22.46 25-29 27.175 25.86 24.585 22.38 30-34 28.34 25.45 24.21 22.0 35-39 28.165000000000003 26.415 23.695 21.725 40-44 27.51 25.569999999999997 24.740000000000002 22.18 45-49 27.85 26.245 23.724999999999998 22.18 50-54 27.725 25.474999999999998 24.565 22.235 55-59 27.41 26.125 24.305 22.16 60-64 27.725 24.985 25.55 21.740000000000002 65-69 28.74 25.290000000000003 24.07 21.9 70-74 28.375 24.735 25.115 21.775 75-79 27.71 24.77 24.995 22.525000000000002 80-84 27.677767776777678 24.897489748974895 25.02250225022502 22.402240224022403 85-89 27.92 25.705 23.53 22.845 90-94 28.18 25.480000000000004 24.13 22.21 95-99 27.145000000000003 24.77 25.195 22.89 100-104 27.29 26.525 24.755 21.43 105-109 28.860202141499048 23.9967977584309 25.08255779045332 22.060442309616732 110-114 27.139999999999997 26.400000000000002 24.485 21.975 115-119 28.050000000000004 25.629999999999995 24.709999999999997 21.61 120-124 28.16 25.965 22.835 23.04 125-129 27.77 25.924999999999997 23.78 22.525000000000002 130-134 28.189999999999998 26.279999999999998 23.565 21.965 135-139 27.939999999999998 25.47 24.47 22.12 140-144 29.13 24.965 24.529999999999998 21.375 145-149 28.27848354506352 26.39291787536261 24.187256176853055 21.141342402720817 150-151 28.971728796597446 25.606705028771582 23.705278959219413 21.71628721541156 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.5 6 0.5 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.5 21 0.5 22 0.5 23 1.0 24 3.0 25 4.5 26 2.5 27 2.5 28 5.5 29 11.5 30 14.5 31 16.0 32 19.0 33 15.0 34 15.0 35 31.5 36 63.5 37 84.5 38 89.0 39 103.5 40 121.0 41 117.0 42 108.5 43 132.0 44 134.5 45 113.5 46 108.5 47 102.0 48 95.0 49 101.5 50 107.5 51 115.0 52 124.0 53 166.5 54 236.5 55 280.0 56 273.0 57 198.0 58 166.0 59 176.5 60 140.5 61 104.5 62 82.5 63 47.0 64 27.0 65 22.5 66 16.0 67 22.0 68 25.0 69 13.0 70 6.5 71 8.0 72 7.5 73 4.5 74 2.0 75 2.0 76 2.5 77 1.5 78 2.0 79 1.5 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.5 86 0.5 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.01 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.06999999999999999 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.03 150-151 0.075 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 66.4 #Duplication Level Percentage of deduplicated Percentage of total 1 76.84487951807229 51.025 2 12.801204819277109 17.0 3 4.631024096385542 9.225 4 2.635542168674699 7.000000000000001 5 1.091867469879518 3.6249999999999996 6 0.48945783132530124 1.95 7 0.5647590361445783 2.625 8 0.37650602409638556 2.0 9 0.15060240963855423 0.8999999999999999 >10 0.41415662650602414 4.65 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GGCTTGCGGTGGATACCTAGGCACCCAGAGACGAGGAAGGGCGTAGCAAG 37 0.9249999999999999 No Hit GTTCGATCCTGGCTCAGGATGAACGCTGGCGGCATGCTTAACACATGCAA 34 0.8500000000000001 No Hit CCTGGCTCAGGATGAACGCTGGCGGCATGCTTAACACATGCAAGTCGAAC 20 0.5 No Hit CCTTACCAGGGCTTGACATGCCGCGAATCCTCTTGAAAGAGAGGGGTGCC 16 0.4 No Hit GTGACCAGGCCCGGGCGACTGTTTACCAAAAACACAGGTCTCCGCAAAGT 14 0.35000000000000003 No Hit CCTGCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGCAA 13 0.325 No Hit GTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGATCTT 11 0.27499999999999997 No Hit ATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAA 11 0.27499999999999997 No Hit CTTGCGGTGGATACCTAGGCACCCAGAGACGAGGAAGGGCGTAGCAAGCG 10 0.25 No Hit ACCAGGTCCAGACATAGCAAGGATTGACAGACTGAGAGCTCTTTCTTGAT 10 0.25 No Hit CTTACCAGGGCTTGACATGCCGCGAATCCTCTTGAAAGAGAGGGGTGCCC 10 0.25 No Hit TGAAAATGAATCTGCTAATGAGGGTTACAAATTTGGTCAAGAGGAAGAGA 9 0.22499999999999998 No Hit GCTTGTTATATGGGTCGTGAGTGGGAACTTAGTTTCCGTTTGGGTATGCG 9 0.22499999999999998 No Hit GTTTTGATGATCCCTACCTTATTGACCGCAACTTCTGTATTTATTATCGC 9 0.22499999999999998 No Hit GTCAAGGTCGCGTTATTAATACTTGGGCTGATATCATCAACCGTGCTAAT 9 0.22499999999999998 No Hit CTCAGGATGAACGCTGGCGGCATGCTTAACACATGCAAGTCGAACGGGAA 8 0.2 No Hit TATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAA 8 0.2 No Hit GTTGAAAATAAGCATAGATCCGGAGATTCCCAAATAGGTCAACCTTTTAA 8 0.2 No Hit ATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCA 8 0.2 No Hit CTTACCAGGTCCAGACATAGCAAGGATTGACAGACTGAGAGCTCTTTCTT 8 0.2 No Hit GTGGGAACTTAGTTTCCGTTTGGGTATGCGCCCTTGGATTGCTGTTGCAT 8 0.2 No Hit AGTGACCAGGCCCGGGCGACTGTTTACCAAAAACACAGGTCTCCGCAAAG 8 0.2 No Hit CCTAGGCACCCAGAGACGAGGAAGGGCGTAGCAAGCGACGAAATGCTTCG 8 0.2 No Hit GGATGATCAGCCACACTGGGACTGAGACACGGCCCAGACTCCTACGGGAG 8 0.2 No Hit GCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGCAAGTA 8 0.2 No Hit CAGGGCTTGACATGCCGCGAATCCTCTTGAAAGAGAGGGGTGCCCTCGGG 7 0.17500000000000002 No Hit TTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAA 7 0.17500000000000002 No Hit GCTAATTGTTCTACACTTCTTACTTGGTGTAGCTTGTTATATGGGTCGTG 7 0.17500000000000002 No Hit GGCTGATCTTCCCCAAGAGTCCACATCGACGGGAAGGTTTGGCACCTCGA 7 0.17500000000000002 No Hit GGAAACTACTGAAAATGAATCTGCTAATGAGGGTTACAAATTTGGTCAAG 7 0.17500000000000002 No Hit GTTAAGTCTCGCAACGAGCGCAACCCTCGTGTTTAGTTGCCACTATGAGT 7 0.17500000000000002 No Hit TTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAG 7 0.17500000000000002 No Hit GTTATTTTGGCCGATTAATCTTCCAATATGCTAGTTTCAACAACTCTCGT 7 0.17500000000000002 No Hit GGCGGGACTACCCGCTGAGTTTAAGCATATAAATAAGCGGAGGAGAAGAA 7 0.17500000000000002 No Hit GTCTGACATGCGTGCGAGTCGACGGGTTCTGAAACCTGGGATGCGCAAGG 7 0.17500000000000002 No Hit GGAAGCTTCTCTGATGGTATGCCTTTAGGAATATCTGGTACTTTCAACTT 7 0.17500000000000002 No Hit CACACGTGCTACAATGGGCGGGACAAAGGGTCGCGATCTCGCGAGGGTGA 7 0.17500000000000002 No Hit GCTGCGACTGCTGTTTTCTTGATTTACCCTATTGGTCAAGGAAGCTTCTC 7 0.17500000000000002 No Hit GTTCAACCTAAATGGATTCAATTTCAACCAATCTGTAGTTGATAGTCAAG 7 0.17500000000000002 No Hit GCTAACTCCAAAAACCCGTCCTCAGTTCGGATTGCAGGCTGCAACTCGCC 7 0.17500000000000002 No Hit GGAATTTCCGGTGGAGCGGTGAAATGCATTGAGATCGGAAAGAACACCAA 6 0.15 No Hit CGGGGCTAAGCGATCTGCCGAAGCTGTGGGATGTCAAAATGCATCGGTAG 6 0.15 No Hit GTTTGGAACCCTGAACAGACCGCCGGTGTTAAGCCGGAGGAAGGAGAGGA 6 0.15 No Hit GCTAGTTTCAACAACTCTCGTTCTTTACACTTCTTCTTGGCTGCTTGGCC 6 0.15 No Hit GGAACAACAACTGGAAACGGTTGCTAATACCCCGTAGGCTGAGGAGCAAA 6 0.15 No Hit GGAAGGCCTACGGGTCGTCAACTTCTTTTCTCGGAGAAGAAACAATGACG 6 0.15 No Hit ATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCTAAATGG 6 0.15 No Hit GCAATAGCTTACCAAGGCGATGATCAGTAGCTGGTCCGAGAGGATGATCA 6 0.15 No Hit GACCAGGCCCGGGCGACTGTTTACCAAAAACACAGGTCTCCGCAAAGTCG 6 0.15 No Hit TCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGA 6 0.15 No Hit CCCAAATAGGTCAACCTTTTAAACTGCCTGCTGAATCCATGAGCAGGCAA 6 0.15 No Hit TGAAGAATGAGCCGGCGACTCATAGGCAGTGGCTTGGTTAAGGGAACGGA 6 0.15 No Hit CTTCCAATATGCTAGTTTCAACAACTCTCGTTCTTTACACTTCTTCTTGG 6 0.15 No Hit AGCACAACATCCTTATGCATCCATTTCACATGTTAGGTGTAGCTGGTGTT 5 0.125 No Hit GCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGTTAACGAAC 5 0.125 No Hit GTTGTGGTTAGGGGTGAAATGCCACTCGAACCCAGAGCTAGCTGGTTCTC 5 0.125 No Hit CTCGTTCTTTACACTTCTTCTTGGCTGCTTGGCCTGTAGTAGGAATCTGG 5 0.125 No Hit GGGTGAGTAACGCGTAAGAACCTGCCCTTGGGAGGGGAACAACAACTGGA 5 0.125 No Hit GGGAAACTACTGAAAATGAATCTGCTAATGAGGGTTACAAATTTGGTCAA 5 0.125 No Hit GCAGCCGCGGTAAGACAGAGGATGCAAGCGTTATCCGGAATGATTGGGCG 5 0.125 No Hit GGCTGCTTGGCCTGTAGTAGGAATCTGGTTCACTGCTTTAGGTATTAGTA 5 0.125 No Hit GGCTTGACATGCCGCGAATCCTCTTGAAAGAGAGGGGTGCCCTCGGGAAC 5 0.125 No Hit ATTTGGTCAAGAGGAAGAGACTTATAATATTGTGGCTGCTCATGGTTATT 5 0.125 No Hit AGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGATCT 5 0.125 No Hit GCCGCGAATCCTCTTGAAAGAGAGGGGTGCCCTCGGGAACGCGGACACAG 5 0.125 No Hit GCTTGCGGTGGATACCTAGGCACCCAGAGACGAGGAAGGGCGTAGCAAGC 5 0.125 No Hit GCTTGACATGCCGCGAATCCTCTTGAAAGAGAGGGGTGCCCTCGGGAACG 5 0.125 No Hit GGGAAGTGGTGTTTCCAGTGGCGAACGGGTGAGTAACGCGTAAGAACCTG 5 0.125 No Hit GGGAACTTAGTTTCCGTTTGGGTATGCGCCCTTGGATTGCTGTTGCATAT 5 0.125 No Hit GTTGGGTTAAGTCTCGCAACGAGCGCAACCCTCGTGTTTAGTTGCCACTA 5 0.125 No Hit ACCTTACCAGGGCTTGACATGCCGCGAATCCTCTTGAAAGAGAGGGGTGC 5 0.125 No Hit GGATAACTAGCACTGAAAATCGTCTTTACATCGGATGGTTCGGTGTTTTG 5 0.125 No Hit CCTGAACAGACCGCCGGTGTTAAGCCGGAGGAAGGAGAGGATGAGGCCAA 5 0.125 No Hit GCACAACATCCTTATGCATCCATTTCACATGTTAGGTGTAGCTGGTGTTT 5 0.125 No Hit AAGGCTTGCGGTGGATACCTAGGCACCCAGAGACGAGGAAGGGCGTAGCA 5 0.125 No Hit GTTTGATCAGGGAAACTACTGAAAATGAATCTGCTAATGAGGGTTACAAA 5 0.125 No Hit GTTCCGACCCGCACGAAAGGCGTAACGATCTGGGCACTGTCTCGGAGAGA 5 0.125 No Hit GTCAAATCCCAGGGCTCAACCCTGGACAGGCGGTGGAAACTACCAAGCTG 5 0.125 No Hit AGAACCTTACCAGGGCTTGACATGCCGCGAATCCTCTTGAAAGAGAGGGG 5 0.125 No Hit GCTTAACACATGCAAGTCGAACGGGAAGTGGTGTTTCCAGTGGCGAACGG 5 0.125 No Hit GGCTTTTCAAGTCCGCCGTCAAATCCCAGGGCTCAACCCTGGACAGGCGG 5 0.125 No Hit GTCGCGATCTCGCGAGGGTGAGCTAACTCCAAAAACCCGTCCTCAGTTCG 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.0125 0.0 0.0 0.0 0.0 70-71 0.05 0.0 0.0 0.0 0.0 72-73 0.05 0.0 0.0 0.0 0.0 74-75 0.07500000000000001 0.0 0.0 0.0 0.0 76-77 0.125 0.0 0.0 0.0 0.0 78-79 0.125 0.0 0.0 0.0 0.0 80-81 0.125 0.0 0.0 0.0 0.0 82-83 0.16249999999999998 0.0 0.0 0.0 0.0 84-85 0.25 0.0 0.0 0.0 0.0 86-87 0.375 0.0 0.0 0.0 0.0 88-89 0.5 0.0 0.0 0.0 0.0 90-91 0.65 0.0 0.0 0.0 0.0 92-93 0.8374999999999999 0.0 0.0 0.0 0.0 94-95 1.1 0.0 0.0 0.0 0.0 96-97 1.3 0.0 0.0 0.0 0.0 98-99 1.5375 0.0 0.0 0.0 0.0 100-101 1.8875 0.0 0.0 0.0 0.0 102-103 2.2625 0.0 0.0 0.0 0.0 104-105 2.5375 0.0 0.0 0.0 0.0 106-107 2.85 0.0 0.0 0.0 0.0 108-109 3.1875 0.0 0.0 0.0 0.0 110-111 3.5625 0.0 0.0 0.0 0.0 112-113 4.0375 0.0 0.0 0.0 0.0 114-115 4.525 0.0 0.0 0.0 0.0 116-117 4.987500000000001 0.0 0.0 0.0 0.0 118-119 5.637499999999999 0.0 0.0 0.0 0.0 120-121 6.4875 0.0 0.0 0.0 0.0 122-123 7.025 0.0 0.0 0.0 0.0 124-125 7.875 0.0 0.0 0.0 0.0 126-127 8.45 0.0 0.0 0.0 0.0 128-129 9.15 0.0 0.0 0.0 0.0 130-131 9.5625 0.0 0.0 0.0 0.0 132-133 10.4 0.0 0.0 0.0 0.0 134-135 11.1375 0.0 0.0 0.0 0.0 136-137 11.6875 0.0 0.0 0.0 0.0 138-139 12.5375 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TTAAGCC 10 0.0063807624 148.29488 145 TTCGGGA 10 0.0068892627 144.5875 2 GGTCGCG 10 0.0068892627 144.5875 6 >>END_MODULE Read 864936 spots for SRR6941604.sra Written 864936 spots for SRR6941604.sra Read 864936 spots for SRR6941604.sra Written 864936 spots for SRR6941604.sra Read 864936 spots for SRR6941604.sra Written 864936 spots for SRR6941604.sra Read 864952 spots for SRR6941604.sra Written 864952 spots for SRR6941604.sra Read 864936 spots for SRR6941604.sra Written 864936 spots for SRR6941604.sra Read 864936 spots for SRR6941604.sra Written 864936 spots for SRR6941604.sra Read 864936 spots for SRR6941604.sra Written 864936 spots for SRR6941604.sra Read 864936 spots for SRR6941604.sra Written 864936 spots for SRR6941604.sra Read 864936 spots for SRR6941604.sra Written 864936 spots for SRR6941604.sra Read 864936 spots for SRR6941604.sra Written 864936 spots for SRR6941604.sra Read 864936 spots for SRR6941604.sra Written 864936 spots for SRR6941604.sra Read 864936 spots for SRR6941604.sra Written 864936 spots for SRR6941604.sra Read 864936 spots for SRR6941604.sra Written 864936 spots for SRR6941604.sra Read 864936 spots for SRR6941604.sra Written 864936 spots for SRR6941604.sra Read 864936 spots for SRR6941604.sra Written 864936 spots for SRR6941604.sra Read 864936 spots for SRR6941604.sra Written 864936 spots for SRR6941604.sra Read 864936 spots for SRR6941604.sra Written 864936 spots for SRR6941604.sra Read 864936 spots for SRR6941604.sra Written 864936 spots for SRR6941604.sra Read 864936 spots for SRR6941604.sra Written 864936 spots for SRR6941604.sra Read 864936 spots for SRR6941604.sra Written 864936 spots for SRR6941604.sra SRR ids: ['SRR6941604.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_wrqweco5 SRR6941604.sra spots: 17298736 blocks: [[1, 864936], [864937, 1729872], [1729873, 2594808], [2594809, 3459744], [3459745, 4324680], [4324681, 5189616], [5189617, 6054552], [6054553, 6919488], [6919489, 7784424], [7784425, 8649360], [8649361, 9514296], [9514297, 10379232], [10379233, 11244168], [11244169, 12109104], [12109105, 12974040], [12974041, 13838976], [13838977, 14703912], [14703913, 15568848], [15568849, 16433784], [16433785, 17298736]] SRR6941604 file size 5840273 SRR6941604 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR6941604 SRR6941604_1.fastq SRR6941604_2.fastq Input file: SRR6941604_1.fastq Paired file: SRR6941604_2.fastq trimmed: SRR6941604-trimmed-pair1.fastq, SRR6941604-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Fri Dec 6 12:53:55 2024 >> started Fri Dec 6 12:54:15 2024 >> done (19.582s) 17298736 read pairs processed; of these: 7946 ( 0.05%) short read pairs filtered out after trimming by size control 8100 ( 0.05%) empty read pairs filtered out after trimming by size control 17282690 (99.91%) read pairs available; of these: 8304856 (48.05%) trimmed read pairs available after processing 8977834 (51.95%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 2 0.00% 19 2 0.00% 20 3 0.00% 21 2 0.00% 22 2 0.00% 23 2 0.00% 24 8 0.00% 25 6 0.00% 26 9 0.00% 27 17 0.00% 28 8 0.00% 29 13 0.00% 30 13 0.00% 31 14 0.00% 32 12 0.00% 33 15 0.00% 34 22 0.00% 35 14 0.00% 36 13 0.00% 37 15 0.00% 38 23 0.00% 39 24 0.00% 40 24 0.00% 41 41 0.00% 42 33 0.00% 43 44 0.00% 44 51 0.00% 45 40 0.00% 46 64 0.00% 47 64 0.00% 48 67 0.00% 49 103 0.00% 50 120 0.00% 51 107 0.00% 52 173 0.00% 53 155 0.00% 54 200 0.00% 55 219 0.00% 56 240 0.00% 57 233 0.00% 58 284 0.00% 59 385 0.00% 60 364 0.00% 61 518 0.00% 62 607 0.00% 63 665 0.00% 64 791 0.00% 65 867 0.01% 66 985 0.01% 67 1077 0.01% 68 1274 0.01% 69 1358 0.01% 70 1588 0.01% 71 1788 0.01% 72 2193 0.01% 73 2520 0.01% 74 2533 0.01% 75 3015 0.02% 76 3289 0.02% 77 3666 0.02% 78 4077 0.02% 79 4923 0.03% 80 5489 0.03% 81 6070 0.04% 82 6599 0.04% 83 7746 0.04% 84 8059 0.05% 85 10309 0.06% 86 10431 0.06% 87 11083 0.06% 88 12621 0.07% 89 13311 0.08% 90 14347 0.08% 91 15353 0.09% 92 17603 0.10% 93 18690 0.11% 94 19178 0.11% 95 21963 0.13% 96 21911 0.13% 97 24348 0.14% 98 24822 0.14% 99 26900 0.16% 100 27550 0.16% 101 30543 0.18% 102 30047 0.17% 103 30907 0.18% 104 32771 0.19% 105 33688 0.19% 106 34933 0.20% 107 36679 0.21% 108 39492 0.23% 109 40791 0.24% 110 40441 0.23% 111 42328 0.24% 112 43335 0.25% 113 43718 0.25% 114 45290 0.26% 115 49121 0.28% 116 50232 0.29% 117 48952 0.28% 118 50599 0.29% 119 49401 0.29% 120 53558 0.31% 121 55792 0.32% 122 57529 0.33% 123 62064 0.36% 124 60043 0.35% 125 65799 0.38% 126 63167 0.37% 127 64680 0.37% 128 63992 0.37% 129 66950 0.39% 130 64406 0.37% 131 68014 0.39% 132 70055 0.41% 133 71332 0.41% 134 73770 0.43% 135 74978 0.43% 136 79451 0.46% 137 78616 0.45% 138 84872 0.49% 139 88918 0.51% 140 90951 0.53% 141 102610 0.59% 142 105695 0.61% 143 114858 0.66% 144 129507 0.75% 145 152970 0.89% 146 178126 1.03% 147 227790 1.32% 148 329355 1.91% 149 635165 3.68% 150 3731208 21.59% 151 8977834 51.95% 17282690 reads passed initial QC criterion=sequence-density sequence-density=1.14 sequence-density-rank=1 fanout-score=2.44 fanout-score-rank=25 prefix-density=1.15 prefix-fanout=2.4 sequence=GTTACGGCCGCCGTTCACCGGGGCTTCGGTCGCCGGCTTCCCTGTCATCAGTTCACCAACTTCCTTGACCTTCCGGCACTGGGCAGGCGTCAGCCCCCATACATGGTCTTACGACTTTGCGGAGACCTGTGTTTTTGGTAAACAGTCGCCCGGGCCTGGTCACTGCGACCCCCTTTTGTGAGGGGGCACCCCTTCT criterion=fanout-score sequence-density=0.01 sequence-density-rank=41 fanout-score=267.76 fanout-score-rank=1 prefix-density=1.86 prefix-fanout=1.0 sequence=GTAGAACAAGATATTGGGTATTTCTCGCTTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCATTAATAGCGGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTACGTTCGTGCATTACTTCCATACCAAGATTAGCACGGTTGATGATATCAGCCCAAGTATTAATAACGCGACCTTGACTATCAACTACAGATTGGTTGAAATTGAATCCATTTAGGTTGAACGCCATAGTACTAATACCTAAAGCAGTGAACCAGATTCCTACTACAGGCCAAGCAGCCAAGAAGAAGTGTAAAGAACGAGAGTTGTTGAAACTAGCATATTGGAAGATTAATCGGCCAAAATAACCATGAGCAGCCACAATATTATAAGTCTCTTCCTCTTGACCAAATTTGTAACCCTCATTAGCAGATTCATTTTCAGTAGTTTCCCTGATCAAACTAGAGGTTACCAAGGAACCATGCATAGCACTGAATAGGGAACCG criterion=sequence-density sequence-density=1.03 sequence-density-rank=1 fanout-score=6.55 fanout-score-rank=8 prefix-density=4.28 prefix-fanout=1.6 sequence=TGGTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGTTAACGAACGAGACCTCAGCCTGCTAACTAGCTATGCGGAGCCATCCCTCCGCAGCTAGCTTCTTAGAGGGACTATCGCCGTTTAGGCGACGGAAGTTTGAGGCAATAACAGGTCTGTGATGCCCTTAGATGTTCTGGGCCGCACGCGCGCTACACTGATGTATTCAACGAGTATATAGCCTTGGCCGACAGGCCCGGGTAATCTTGGGAAATTTCATCGTGATGGGGATAGATCATTGCAATTGTTGGTCTTCAACGAGGAATGCCTAGTAAGCGCGAGTCATCAGCTCGCGTTGACTACGTCCCTGCCCTTTGTACACACC criterion=fanout-score sequence-density=0.02 sequence-density-rank=36 fanout-score=85.21 fanout-score-rank=1 prefix-density=1.23 prefix-fanout=1.1 sequence=AGAAGGGGTGCCCCCTCACAAAAGGGGGTCGCAGTGACCAGGCCCGGGCGACTGTTTACCAAAAACACAGGTCTCCGCAAAGTCGTAAGACCATGTATGGGGGCTGACGCCTGCCCAGTGCCGGAAGGTCAAGGAAGTTGGTGAACTGATGACAGGGAAGCCGGCGACCGAAGCCCCGGTGAACGGCGGCCGTAAC SRR6941604 testing PE reads STAR mapping to Ensembl genome Started job on | Dec 06 12:54:45 Started mapping on | Dec 06 12:54:45 Finished on | Dec 06 12:56:15 Mapping speed, Million of reads per hour | 691.31 Number of input reads | 17282690 Average input read length | 291 UNIQUE READS: Uniquely mapped reads number | 7701273 Uniquely mapped reads % | 44.56% Average mapped length | 294.69 Number of splices: Total | 861145 Number of splices: Annotated (sjdb) | 761338 Number of splices: GT/AG | 807775 Number of splices: GC/AG | 10718 Number of splices: AT/AC | 3224 Number of splices: Non-canonical | 39428 Mismatch rate per base, % | 0.15% Deletion rate per base | 0.01% Deletion average length | 1.55 Insertion rate per base | 0.00% Insertion average length | 1.72 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 6250208 % of reads mapped to multiple loci | 36.16% Number of reads mapped to too many loci | 478058 % of reads mapped to too many loci | 2.77% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 3.02% % of reads unmapped: other | 13.49% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 3334991 3334991 3334991 N_multimapping 6250208 6250208 6250208 N_noFeature 5025731 7554821 5086576 N_ambiguous 179499 1537 96599 UnstrandedReadsAssigned:2496043 PositiveStrandReadsAssigned:144915 NegativeStrandReadsAssigned:2518098 Dataset is classified negative stranded MeadianReadLen=151 20thPercentileLength=149 echo kmer=145 SRR6941604 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,972 [index] number of k-mers: 66,720,672 [index] number of equivalence classes: 111,837 [quant] running in paired-end mode [quant] will process pair 1: SRR6941604-trimmed-pair1.fastq SRR6941604-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 17,282,690 reads, 5,065,082 reads pseudoaligned [quant] estimated average fragment length: 203.728 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 943 rounds 52973 SRR6941604.ke.tsv 35125 SRR6941604.se.tsv 88098 total ==> SRR6941604.ke.tsv <== target_id length eff_length est_counts tpm PNS24245 936 733.376 0 0 PNS24247 1044 841.272 5.10077 0.588019 PNS24249 1928 1725.27 7.73042 0.434548 PNS24246 1044 841.272 5.10077 0.588019 PNS24248 1044 841.272 5.10077 0.588019 PNS24244 1471 1268.27 5.96726 0.456305 PNS24243 293 115.911 0 0 KQK14069 1603 1400.27 1145.56 79.3409 KQK14071 474 278.254 22.9654 8.00433 ==> SRR6941604.se.tsv <== BRADI_1g14170v3 1440 BRADI_1g53295v3 4 BRADI_1g59795v3 28 BRADI_1g07683v3 0 BRADI_1g00485v3 0 BRADI_1g20270v3 18 BRADI_1g74790v3 5 BRADI_1g09890v3 0 BRADI_1g77505v3 14 BRADI_1g48960v3 0 SRR6941604 completed mapping pipeline successfully