Starting /dee2/code/volunteer_pipeline.sh SRR6941605 current disk space = 1551099670528 free memory = 1604627520 SRR6941605 SRAfilesize cf591e6aaaa623f6b4cf42f1f643779a SRR6941605.sra SRR6941605.sra file validated SRR6941605 is paired end SRR6941605 is conventional basespace SRR6941605 read1 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR6941605_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 50 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.914 34.0 33.0 34.0 32.0 34.0 2 33.19525 34.0 33.0 34.0 32.0 34.0 3 33.2225 34.0 33.0 34.0 32.0 34.0 4 33.36975 34.0 33.0 34.0 33.0 34.0 5 33.486 34.0 33.0 34.0 33.0 34.0 6 37.33125 38.0 38.0 38.0 36.0 38.0 7 37.6375 38.0 38.0 38.0 38.0 38.0 8 37.68325 38.0 38.0 38.0 38.0 38.0 9 37.70575 38.0 38.0 38.0 38.0 38.0 10-14 37.67444999999999 38.0 38.0 38.0 38.0 38.0 15-19 37.68595 38.0 38.0 38.0 38.0 38.0 20-24 37.6794 38.0 38.0 38.0 38.0 38.0 25-29 37.60935 38.0 38.0 38.0 38.0 38.0 30-34 37.5262 38.0 38.0 38.0 38.0 38.0 35-39 37.591750000000005 38.0 38.0 38.0 38.0 38.0 40-44 37.58555 38.0 38.0 38.0 38.0 38.0 45-49 37.62295 38.0 38.0 38.0 38.0 38.0 50-54 37.5878 38.0 38.0 38.0 38.0 38.0 55-59 37.56615 38.0 38.0 38.0 38.0 38.0 60-64 37.53835 38.0 38.0 38.0 38.0 38.0 65-69 37.397949999999994 38.0 38.0 38.0 37.4 38.0 70-74 37.419050000000006 38.0 38.0 38.0 37.2 38.0 75-79 37.45355000000001 38.0 38.0 38.0 37.6 38.0 80-84 37.39545 38.0 38.0 38.0 37.4 38.0 85-89 37.23435 38.0 38.0 38.0 36.8 38.0 90-94 37.266299999999994 38.0 38.0 38.0 37.0 38.0 95-99 37.16285 38.0 38.0 38.0 36.0 38.0 100-104 37.17655 38.0 38.0 38.0 36.0 38.0 105-109 37.05905 38.0 38.0 38.0 35.8 38.0 110-114 36.58715 38.0 38.0 38.0 34.6 38.0 115-119 36.625249999999994 38.0 38.0 38.0 34.8 38.0 120-124 36.67764999999999 38.0 38.0 38.0 35.0 38.0 125-129 36.765 38.0 38.0 38.0 35.0 38.0 130-134 36.6594 38.0 38.0 38.0 34.6 38.0 135-139 36.42935 38.0 38.0 38.0 34.0 38.0 140-144 36.2162 38.0 38.0 38.0 33.2 38.0 145-149 35.908500000000004 38.0 38.0 38.0 32.4 38.0 150-151 32.26925 35.5 32.5 38.0 16.5 38.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 1.0 4 0.0 5 1.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 1.0 18 1.0 19 5.0 20 1.0 21 2.0 22 1.0 23 2.0 24 2.0 25 3.0 26 11.0 27 5.0 28 6.0 29 17.0 30 27.0 31 28.0 32 37.0 33 59.0 34 84.0 35 159.0 36 393.0 37 3154.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 38.0814717477004 11.61629434954008 6.8856767411300925 43.416557161629434 2 24.137068534267133 13.406703351675839 34.017008504252125 28.4392196098049 3 19.475 19.6 26.900000000000002 34.025 4 26.424999999999997 27.325 21.099999999999998 25.15 5 24.45 31.324999999999996 21.85 22.375 6 20.625 31.65 24.099999999999998 23.625 7 16.45 20.349999999999998 43.0 20.200000000000003 8 19.2 19.025 29.975 31.8 9 18.55 19.05 32.475 29.925 10-14 22.46 24.91 24.13 28.499999999999996 15-19 22.595000000000002 23.54 26.669999999999998 27.195000000000004 20-24 23.119999999999997 24.035 26.14 26.705000000000002 25-29 22.87114355717786 22.72113605680284 26.286314315715785 28.121406070303518 30-34 22.720000000000002 24.305 25.3 27.675 35-39 22.41224122412241 23.1973197319732 26.247624762476246 28.14281428142814 40-44 23.897169150745224 23.887166149844955 25.71771531459438 26.497949384815445 45-49 21.44 23.465 27.125 27.97 50-54 22.776833049914973 22.876863058917678 26.062818845653695 28.283485045513657 55-59 22.56338450767615 23.91358703805571 26.143921588238232 27.379106866029908 60-64 22.842284228422844 22.767276727672765 26.672667266726673 27.71777177717772 65-69 22.245 24.205 25.509999999999998 28.04 70-74 23.517351735173516 23.607360736073606 25.15751575157516 27.71777177717772 75-79 22.475 24.560000000000002 25.25 27.715 80-84 23.8973897389739 24.182418241824184 24.897489748974895 27.022702270227022 85-89 22.651132556627832 23.2061603080154 25.721286064303218 28.421421071053555 90-94 23.07730773077308 24.51245124512451 24.462446244624463 27.947794779477945 95-99 22.325 24.295 25.005 28.375 100-104 22.54789176211674 23.54324013404692 25.21882658930626 28.690041514530083 105-109 22.965 23.305 25.96 27.77 110-114 23.346225325229796 24.14988196293134 25.52112110100959 26.982771610829275 115-119 23.208464971666416 24.437089413770625 24.497266937465522 27.857178677097437 120-124 23.09078170353318 25.232709438494645 23.66629966970273 28.010209188269442 125-129 23.504700940188037 25.1000200040008 23.564712942588518 27.830566113222645 130-134 23.862386238623863 24.222422242224223 23.827382738273826 28.08780878087809 135-139 23.225 24.645 24.875 27.255000000000003 140-144 23.98 24.59 23.815 27.615000000000002 145-149 22.78 24.72 24.47 28.03 150-151 21.3125 25.825 23.974999999999998 28.8875 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.5 2 0.5 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.5 21 1.0 22 1.5 23 1.5 24 1.5 25 1.5 26 1.5 27 3.0 28 5.0 29 7.5 30 9.0 31 9.0 32 10.5 33 12.0 34 17.0 35 24.0 36 44.0 37 85.5 38 91.0 39 86.5 40 100.0 41 98.0 42 88.5 43 94.0 44 102.5 45 110.0 46 115.5 47 100.5 48 92.5 49 110.0 50 152.5 51 180.0 52 176.5 53 188.0 54 240.5 55 319.5 56 313.0 57 235.0 58 202.5 59 160.0 60 112.5 61 79.5 62 51.5 63 41.5 64 27.5 65 17.5 66 12.0 67 10.5 68 9.0 69 6.5 70 6.0 71 5.5 72 5.5 73 3.5 74 3.5 75 5.0 76 6.0 77 3.0 78 0.5 79 0.5 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 4.875 2 0.05 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.005 30-34 0.0 35-39 0.01 40-44 0.03 45-49 0.0 50-54 0.03 55-59 0.015 60-64 0.01 65-69 0.0 70-74 0.01 75-79 0.0 80-84 0.01 85-89 0.005 90-94 0.01 95-99 0.0 100-104 0.034999999999999996 105-109 0.0 110-114 0.455 115-119 0.295 120-124 0.09 125-129 0.02 130-134 0.01 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 68.125 #Duplication Level Percentage of deduplicated Percentage of total 1 77.79816513761469 53.0 2 12.293577981651376 16.75 3 4.4036697247706424 9.0 4 2.495412844036697 6.800000000000001 5 1.1376146788990826 3.875 6 0.7706422018348624 3.15 7 0.40366972477064217 1.925 8 0.11009174311926606 0.6 9 0.25688073394495414 1.575 >10 0.3302752293577982 3.325 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCATTAAT 29 0.7250000000000001 No Hit GTACAAGGCCCGGGAACGGATTCACCGCCGTATGGCTGACCGGCGATTAC 19 0.475 No Hit GTCGGTTTCGGGTACAGGTACCCTTTTGTTGAAGGTCGTTCGAGCTTTTC 16 0.4 No Hit CCCGACTGTCCCTATTAATCATTACTCCGATCCCGAAGGCCAACACAATA 14 0.35000000000000003 No Hit CCTAGATGTCCAGTCAACTGCTGCGCCTCAACGCATTTCGGGGAGAACCA 12 0.3 No Hit GTTGAAACTAGCATATTGGAAGATTAATCGGCCAAAATAACCATGAGCAG 12 0.3 No Hit GGGCTTACTACTTATATGCTTTCAGCAGTTATCCTCTCCGCACTTGGCTA 11 0.27499999999999997 No Hit CCCGAAGTTACGGGGCTATTTTGCCGAGTTCCTTAGAGAGAGTTGTCTCG 10 0.25 No Hit CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCA 10 0.25 No Hit GTACAAAGGGCAGGGACGTAGTCAACGCGAGCTGATGACTCGCGCTTACT 9 0.22499999999999998 No Hit CACATGCTCCACCGCTTGTGCGGGCCCCCGTCAATTCCTTTGAGTTTCAT 9 0.22499999999999998 No Hit CCTCACGGTACTACTTCGCTATCGGTCACCCAGGAGTATTTAGCCTTGCA 9 0.22499999999999998 No Hit GCCAGCTCCTATAGTGTGACGGGCGGTGTGTACAAGGCCCGGGAACGGAT 9 0.22499999999999998 No Hit CTTTCCCTCACGGTACTACTTCGCTATCGGTCACCCAGGAGTATTTAGCC 9 0.22499999999999998 No Hit GTCGGTTCGGACCTCTGCTTAGTTTCATCCAAGCTTCATCCTGGTCATGG 9 0.22499999999999998 No Hit GGCAATTTCAAGCACTCTTTGACTCTCTTTTCAAAGTCCTTTTCATCTTT 9 0.22499999999999998 No Hit CGGGCGGTGTGTACAAGGCCCGGGAACGGATTCACCGCCGTATGGCTGAC 8 0.2 No Hit GTAACTTTTATCCGTTGAGCGACGGCCCTTCCACTCGGCACCGTCGGATC 8 0.2 No Hit GTTTACGGCTAGGACTACTGGGGTCTCTAATCCCATTTGCTCCCCTAGCT 8 0.2 No Hit CACCGCTTGTGCGGGCCCCCGTCAATTCCTTTGAGTTTCATTCTTGCGAA 7 0.17500000000000002 No Hit CCCATATAACAAGCTACACCAAGTAAGAAGTGTAGAACAATTAGCTCATA 7 0.17500000000000002 No Hit GGCGCATACCCAAACGGAAACTAAGTTCCCACTCACGACCCATATAACAA 7 0.17500000000000002 No Hit GGCATGATGACTTGGCCTCATCCTCTCCTTCCTCCGGCTTAACACCGGCG 7 0.17500000000000002 No Hit GTCAATTCCTTTGAGTTTCATTCTTGCGAACGTACTCCCCAGGCGGGATA 7 0.17500000000000002 No Hit CACTCATCTTGGGGTGGGCTTACTACTTATATGCTTTCAGCAGTTATCCT 7 0.17500000000000002 No Hit CATGCTCCACCGCTTGTGCGGGCCCCCGTCAATTCCTTTGAGTTTCATTC 7 0.17500000000000002 No Hit TTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCATTAATA 7 0.17500000000000002 No Hit CTCATAAGGACCACCATTGTATAACCATTCATCAACGGATGCAGCTTCCC 7 0.17500000000000002 No Hit CAAAGGGCAGGGACGTAGTCAACGCGAGCTGATGACTCGCGCTTACTAGG 7 0.17500000000000002 No Hit GCGGAAAAACCTTATCCATTAATAGCGGGAACTTCAAGAGCAGCTAGATC 7 0.17500000000000002 No Hit GTTGAAGACCAACAATTGCAATGATCTATCCCCATCACGATGAAATTTCC 6 0.15 No Hit GTCAATTCCTTTAAGTTTCAGCCTTGCGACCATACTCCCCCCGGAACCCA 6 0.15 No Hit GTCGCAAGCCTAGCCTTAGGCATCCCCCTCCTTACGGTTAAGGGTAATGA 6 0.15 No Hit CCTAGAGTAACTTTTATCCGTTGAGCGACGGCCCTTCCACTCGGCACCGT 6 0.15 No Hit AGACGCTTTACGCCCAATCATTCCGGATAACGCTTGCATCCTCTGTCTTA 6 0.15 No Hit ACATGCTCCACCGCTTGTGCGGGCCCCCGTCAATTCCTTTGAGTTTCATT 6 0.15 No Hit GCTCCACCGCTTGTGCGGGCCCCCGTCAATTCCTTTGAGTTTCATTCTTG 6 0.15 No Hit CCAGATTCCTACTACAGGCCAAGCAGCCAAGAAGAAGTGTAAAGAACGAG 6 0.15 No Hit GTTACGACTTCTCCTTCCTCTAAATGATAAGGTTCAATGGACTTCTCGCG 6 0.15 No Hit CTTGGGGTGGGCTTACTACTTATATGCTTTCAGCAGTTATCCTCTCCGCA 6 0.15 No Hit GTGTGTACAAGGCCCGGGAACGGATTCACCGCCGTATGGCTGACCGGCGA 6 0.15 No Hit GTATTCTCTACCTACCCACCTGTGTCGGTTTCGGGTACAGGTACCCTTTT 6 0.15 No Hit CGGGCGGTGTGTACAAAGGGCAGGGACGTAGTCAACGCGAGCTGATGACT 6 0.15 No Hit CCCACTGCTGCCTCCCGTAGGAGTCTGGGCCGTGTCTCAGTCCCAGTGTG 6 0.15 No Hit CCTTAACCAAGCCACTGCCTATGAGTCGCCGGCTCATTCTTCAACAGGCA 6 0.15 No Hit GCCAAAATAACCATGAGCAGCCACAATATTATAAGTCTCTTCCTCTTGAC 6 0.15 No Hit GGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTACGTT 6 0.15 No Hit GTTGTCTCTTGCCTGCTCATGGATTCAGCAGGCAGTTTAAAAGGTTGACC 6 0.15 No Hit CCTGTGTCGGTTTCGGGTACAGGTACCCTTTTGTTGAAGGTCGTTCGAGC 6 0.15 No Hit CTTTCTTTTCCTCTGGCTACTAAGATGTTTCAGTTCGCCAGGTTGTCTCT 6 0.15 No Hit GTGGGCTTACTACTTATATGCTTTCAGCAGTTATCCTCTCCGCACTTGGC 6 0.15 No Hit GTGCAATCCGATTGCCGCAGAAGTAGGAATAATAGCACCAGAGATAATAT 5 0.125 No Hit CACCTTTCCCTCACGGTACTACTTCGCTATCGGTCACCCAGGAGTATTTA 5 0.125 No Hit AGATAATATTGTTTCCATAAAGTAAAGAACCAGAAACAGGCTCGCGAATA 5 0.125 No Hit GGAAAAACCTTATCCATTAATAGCGGGAACTTCAAGAGCAGCTAGATCTA 5 0.125 No Hit CACGTGTGTCGCCCAGGGCATAAGGGGCATGATGACTTGGCCTCATCCTC 5 0.125 No Hit GTCAGTGTCGGCCCAGCAGAGTGCTTTCGCCGTTGGTGTTCTTTCCGATC 5 0.125 No Hit ATGAAGACTCGCTTTCGCTACGGCTCCGGTGGGTTCCGTTCCCTTAACCA 5 0.125 No Hit GTCGCAGCTGCAACAGGAGCTGAATATGCAACAGCAATCCAAGGGCGCAT 5 0.125 No Hit GGCGATAATAAATACAGAAGTTGCGGTCAATAAGGTAGGGATCATCAAAA 5 0.125 No Hit ATAAGGACCACCATTGTATAACCATTCATCAACGGATGCAGCTTCCCAAA 5 0.125 No Hit GCCGACTTTCGTCTCTGCTCGACGGGTGAGTCTTGCAGTCAAGCTCCCTT 5 0.125 No Hit CTCATCTTGGGGTGGGCTTACTACTTATATGCTTTCAGCAGTTATCCTCT 5 0.125 No Hit GGATAGATCACCCAGGTTCGGGTCCATAAGCAGTGACAATCGCCCTATGA 5 0.125 No Hit GTCGGCATCGTTTATGGTTGAGACTAGGACGGTATCTGATCGTCTTCGAG 5 0.125 No Hit CGGGAACGGATTCACCGCCGTATGGCTGACCGGCGATTACTAGCGATTCC 5 0.125 No Hit CTCACCAACTAGCTAATCAGACGCGAGCCCCTCCTTGGGCGGATTTCTCC 5 0.125 No Hit AGGCGGGATACTTAACGCGTTAGCTACAGCACTGCACGGGTCGAGTCGCA 5 0.125 No Hit GCGACCTTGACTATCAACTACAGATTGGTTGAAATTGAATCCATTTAGGT 5 0.125 No Hit GGTCAATAAGGTAGGGATCATCAAAACACCGAACCATCCGATGTAAAGAC 5 0.125 No Hit ACAAAGGGCAGGGACGTAGTCAACGCGAGCTGATGACTCGCGCTTACTAG 5 0.125 No Hit CTTAAACCTATAACCATCTTTCGGCTAACCTAGCCTCCTCCGTCCCTCCG 5 0.125 No Hit GTCGAGTTATCATGAATCATCGGATCAGCGAGCAAAGCCCGCGTCAGCCT 5 0.125 No Hit CCTGCTTCATGCAGGCGAGTTGCAGCCTGCAATCCGAACTGAGGACGGGT 5 0.125 No Hit CTCAGATACCGTCATTGTTTCTTCTCCGAGAAAAGAAGTTGACGACCCGT 5 0.125 No Hit CTAAGAAGCTAGCTGCGGAGGGATGGCTCCGCATAGCTAGTTAGCAGGCT 5 0.125 No Hit CTTCACTCCAGTCGCAAGCCTAGCCTTAGGCATCCCCCTCCTTACGGTTA 5 0.125 No Hit CCGGAACCCAAAGACTTTGATTTCTCATAAGGTGCCGGCGGAGTCCTATA 5 0.125 No Hit CTTTTCCTCTGGCTACTAAGATGTTTCAGTTCGCCAGGTTGTCTCTTGCC 5 0.125 No Hit CCCCACTGCTGCCTCCCGTAGGAGTCTGGGCCGTGTCTCAGTCCCAGTGT 5 0.125 No Hit CTCAGCCTACGGGGTATTAGCAACCGTTTCCAGTTGTTGTTCCCCTCCCA 5 0.125 No Hit ACCACATGCTCCACCGCTTGTGCGGGCCCCCGTCAATTCCTTTGAGTTTC 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0125 0.0 0.0 0.0 0.0 68-69 0.025 0.0 0.0 0.0 0.0 70-71 0.05 0.0 0.0 0.0 0.0 72-73 0.05 0.0 0.0 0.0 0.0 74-75 0.05 0.0 0.0 0.0 0.0 76-77 0.0625 0.0 0.0 0.0 0.0 78-79 0.125 0.0 0.0 0.0 0.0 80-81 0.15 0.0 0.0 0.0 0.0 82-83 0.2 0.0 0.0 0.0 0.0 84-85 0.25 0.0 0.0 0.0 0.0 86-87 0.4375 0.0 0.0 0.0 0.0 88-89 0.5375 0.0 0.0 0.0 0.0 90-91 0.6875 0.0 0.0 0.0 0.0 92-93 0.875 0.0 0.0 0.0 0.0 94-95 0.975 0.0 0.0 0.0 0.0 96-97 1.1625 0.0 0.0 0.0 0.0 98-99 1.4 0.0 0.0 0.0 0.0 100-101 1.725 0.0 0.0 0.0 0.0 102-103 2.05 0.0 0.0 0.0 0.0 104-105 2.4125 0.0 0.0 0.0 0.0 106-107 2.7625 0.0 0.0 0.0 0.0 108-109 3.1125 0.0 0.0 0.0 0.0 110-111 3.675 0.0 0.0 0.0 0.0 112-113 4.1625 0.0 0.0 0.0 0.0 114-115 4.625 0.0 0.0 0.0 0.0 116-117 5.1 0.0 0.0 0.0 0.0 118-119 5.65 0.0 0.0 0.0 0.0 120-121 6.2625 0.0 0.0 0.0 0.0 122-123 6.775 0.0 0.0 0.0 0.0 124-125 7.5125 0.0 0.0 0.0 0.0 126-127 8.2125 0.0 0.0 0.0 0.0 128-129 8.7625 0.0 0.0 0.0 0.0 130-131 9.5 0.0 0.0 0.0 0.0 132-133 10.0375 0.0 0.0 0.0 0.0 134-135 10.7875 0.0 0.0 0.0 0.0 136-137 11.524999999999999 0.0 0.0 0.0 0.0 138-139 12.225 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCCGGAT 10 0.0068874825 144.6 7 AGAGATC 20 3.6273568E-4 108.45 145 >>END_MODULE SRR6941605 read2 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR6941605_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 50 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 33.27725 34.0 33.0 34.0 33.0 34.0 2 33.38375 34.0 33.0 34.0 33.0 34.0 3 33.4215 34.0 33.0 34.0 33.0 34.0 4 33.39 34.0 33.0 34.0 33.0 34.0 5 33.352 34.0 33.0 34.0 33.0 34.0 6 37.5825 38.0 38.0 38.0 38.0 38.0 7 37.60925 38.0 38.0 38.0 38.0 38.0 8 37.57575 38.0 38.0 38.0 38.0 38.0 9 37.52925 38.0 38.0 38.0 38.0 38.0 10-14 37.5506 38.0 38.0 38.0 38.0 38.0 15-19 37.52695 38.0 38.0 38.0 38.0 38.0 20-24 37.50065 38.0 38.0 38.0 38.0 38.0 25-29 37.470150000000004 38.0 38.0 38.0 38.0 38.0 30-34 37.5187 38.0 38.0 38.0 38.0 38.0 35-39 37.5052 38.0 38.0 38.0 38.0 38.0 40-44 37.4707 38.0 38.0 38.0 38.0 38.0 45-49 37.4743 38.0 38.0 38.0 38.0 38.0 50-54 37.4857 38.0 38.0 38.0 38.0 38.0 55-59 37.4172 38.0 38.0 38.0 38.0 38.0 60-64 37.336 38.0 38.0 38.0 37.8 38.0 65-69 37.311099999999996 38.0 38.0 38.0 37.2 38.0 70-74 37.334250000000004 38.0 38.0 38.0 37.0 38.0 75-79 37.3091 38.0 38.0 38.0 37.0 38.0 80-84 37.24525 38.0 38.0 38.0 37.0 38.0 85-89 37.232150000000004 38.0 38.0 38.0 36.6 38.0 90-94 37.18175 38.0 38.0 38.0 37.0 38.0 95-99 37.079899999999995 38.0 38.0 38.0 36.0 38.0 100-104 36.80845000000001 38.0 38.0 38.0 35.0 38.0 105-109 36.6675 38.0 38.0 38.0 34.8 38.0 110-114 36.3474 38.0 38.0 38.0 34.2 38.0 115-119 36.2346 38.0 38.0 38.0 33.8 38.0 120-124 36.27755 38.0 38.0 38.0 33.8 38.0 125-129 36.312000000000005 38.0 38.0 38.0 34.0 38.0 130-134 36.37555 38.0 38.0 38.0 34.0 38.0 135-139 36.038850000000004 38.0 38.0 38.0 33.0 38.0 140-144 35.5513 38.0 36.2 38.0 31.2 38.0 145-149 34.62595 38.0 35.8 38.0 28.8 38.0 150-151 30.334 35.5 29.0 38.0 12.5 38.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 2.0 4 3.0 5 0.0 6 0.0 7 1.0 8 1.0 9 0.0 10 1.0 11 0.0 12 1.0 13 1.0 14 1.0 15 0.0 16 0.0 17 2.0 18 0.0 19 3.0 20 3.0 21 4.0 22 3.0 23 5.0 24 4.0 25 5.0 26 10.0 27 13.0 28 10.0 29 23.0 30 26.0 31 28.0 32 43.0 33 73.0 34 114.0 35 187.0 36 451.0 37 2979.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 44.324999999999996 15.55 10.45 29.675 2 31.95 18.224999999999998 29.875 19.950000000000003 3 23.65 21.675 31.175000000000004 23.5 4 28.95 30.625000000000004 21.8 18.625 5 30.525000000000002 32.074999999999996 19.225 18.175 6 25.275 33.85 19.45 21.425 7 22.875 19.85 34.725 22.55 8 25.525 22.275 24.925 27.275 9 26.575 21.8 26.575 25.05 10-14 28.610000000000003 24.345 23.02 24.025 15-19 27.925 25.695 23.794999999999998 22.585 20-24 28.444999999999997 25.035 23.96 22.56 25-29 28.852885288528853 25.292529252925295 23.407340734073408 22.447244724472448 30-34 27.996399819990998 26.306315315765787 23.726186309315466 21.971098554927746 35-39 28.28282828282828 26.472647264726472 23.22732273227323 22.017201720172018 40-44 28.46 26.384999999999998 23.080000000000002 22.075 45-49 27.92639631981599 26.416320816040802 23.081154057702886 22.57612880644032 50-54 28.356417820891046 25.346267313365665 24.046202310115504 22.25111255562778 55-59 27.815 26.165 23.695 22.325 60-64 28.666433321666084 24.491224561228062 24.46122306115306 22.381119055952798 65-69 29.176458822941147 25.591279563978198 22.926146307315364 22.30611530576529 70-74 28.795759151830364 24.42988597719544 23.8247649529906 22.949589917983594 75-79 28.48642432121606 25.321266063303167 23.656182809140457 22.536126806340317 80-84 28.269894463062073 24.82869004151453 23.383184114440052 23.518231380983345 85-89 28.72143607180359 24.931246562328116 23.44617230861543 22.90114505725286 90-94 28.85644282214111 25.5612780639032 23.406170308515424 22.176108805440272 95-99 29.14 24.725 23.79 22.345000000000002 100-104 28.257064266066518 26.351587896974245 23.820955238809702 21.57039259814954 105-109 28.6100405425697 24.665899194153862 24.34055758546474 22.383502677811702 110-114 28.111405570278514 25.83129156457823 23.70618530926546 22.351117555877796 115-119 29.481474073703684 25.336266813340668 23.576178808940448 21.6060803040152 120-124 28.42 26.215 22.6 22.765 125-129 28.371418570928547 26.4063203160158 22.62113105655283 22.601130056502825 130-134 28.754313146972045 26.91903785567835 22.548382257338602 21.778266740011002 135-139 28.186409320466023 26.01630081504075 23.84119205960298 21.956097804890245 140-144 29.717971797179715 25.40754075407541 23.672367236723673 21.202120212021203 145-149 29.091636654661862 26.87575030012005 23.264305722288917 20.76830732292917 150-151 29.316816816816814 26.426426426426424 23.04804804804805 21.20870870870871 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.5 6 0.5 7 0.0 8 0.0 9 0.0 10 0.0 11 0.5 12 0.5 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.5 20 0.5 21 0.0 22 0.5 23 1.0 24 2.0 25 2.0 26 3.0 27 4.0 28 4.0 29 8.0 30 12.0 31 10.5 32 11.0 33 18.0 34 29.5 35 33.5 36 38.0 37 61.0 38 79.5 39 84.0 40 93.0 41 98.5 42 96.0 43 114.5 44 120.5 45 101.5 46 102.0 47 103.5 48 95.5 49 100.5 50 108.5 51 131.5 52 136.0 53 162.0 54 247.5 55 313.0 56 293.0 57 214.0 58 188.5 59 181.0 60 137.0 61 107.0 62 89.0 63 62.0 64 40.0 65 25.0 66 18.0 67 22.5 68 29.5 69 19.0 70 9.0 71 8.0 72 5.0 73 4.0 74 4.5 75 6.5 76 4.5 77 2.0 78 2.0 79 0.5 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.01 30-34 0.005 35-39 0.01 40-44 0.0 45-49 0.005 50-54 0.005 55-59 0.0 60-64 0.005 65-69 0.005 70-74 0.02 75-79 0.005 80-84 0.034999999999999996 85-89 0.005 90-94 0.005 95-99 0.0 100-104 0.025 105-109 0.105 110-114 0.005 115-119 0.005 120-124 0.0 125-129 0.005 130-134 0.015 135-139 0.005 140-144 0.01 145-149 0.04 150-151 0.1 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 67.7 #Duplication Level Percentage of deduplicated Percentage of total 1 76.88330871491877 52.05 2 13.58936484490399 18.4 3 4.763663220088627 9.675 4 1.9940915805022157 5.4 5 1.2186115214180206 4.125 6 0.51698670605613 2.1 7 0.22156573116691286 1.05 8 0.258493353028065 1.4000000000000001 9 0.14771048744460857 0.8999999999999999 >10 0.40620384047267355 4.9 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTTCGATCCTGGCTCAGGATGAACGCTGGCGGCATGCTTAACACATGCAA 46 1.15 No Hit GGCTTGCGGTGGATACCTAGGCACCCAGAGACGAGGAAGGGCGTAGCAAG 35 0.8750000000000001 No Hit CCTTACCAGGGCTTGACATGCCGCGAATCCTCTTGAAAGAGAGGGGTGCC 20 0.5 No Hit GTGACCAGGCCCGGGCGACTGTTTACCAAAAACACAGGTCTCCGCAAAGT 13 0.325 No Hit ATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAA 13 0.325 No Hit CTTACCAGGTCCAGACATAGCAAGGATTGACAGACTGAGAGCTCTTTCTT 13 0.325 No Hit CCTAGGCACCCAGAGACGAGGAAGGGCGTAGCAAGCGACGAAATGCTTCG 13 0.325 No Hit CTTACCAGGGCTTGACATGCCGCGAATCCTCTTGAAAGAGAGGGGTGCCC 12 0.3 No Hit GTCTGACATGCGTGCGAGTCGACGGGTTCTGAAACCTGGGATGCGCAAGG 11 0.27499999999999997 No Hit CCTGCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGCAA 10 0.25 No Hit GGCGGGACTACCCGCTGAGTTTAAGCATATAAATAAGCGGAGGAGAAGAA 10 0.25 No Hit CCTGGCTCAGGATGAACGCTGGCGGCATGCTTAACACATGCAAGTCGAAC 9 0.22499999999999998 No Hit GCTTGCGGTGGATACCTAGGCACCCAGAGACGAGGAAGGGCGTAGCAAGC 9 0.22499999999999998 No Hit GTGGGAACTTAGTTTCCGTTTGGGTATGCGCCCTTGGATTGCTGTTGCAT 9 0.22499999999999998 No Hit CCTGAACAGACCGCCGGTGTTAAGCCGGAGGAAGGAGAGGATGAGGCCAA 9 0.22499999999999998 No Hit GTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGATCTT 8 0.2 No Hit CTTGCGGTGGATACCTAGGCACCCAGAGACGAGGAAGGGCGTAGCAAGCG 8 0.2 No Hit GGGAATTTCCGGTGGAGCGGTGAAATGCATTGAGATCGGAAAGAACACCA 8 0.2 No Hit GGAAGGCCTACGGGTCGTCAACTTCTTTTCTCGGAGAAGAAACAATGACG 8 0.2 No Hit CGGACATTGGTCCTCGAGTGCAAAGGCAGAAGGGAGCTTGACTGCAAGAC 8 0.2 No Hit GGAGTCTGACATGCGTGCGAGTCGACGGGTTCTGAAACCTGGGATGCGCA 8 0.2 No Hit AGAACCTTACCAGGGCTTGACATGCCGCGAATCCTCTTGAAAGAGAGGGG 8 0.2 No Hit CAGGGCTTGACATGCCGCGAATCCTCTTGAAAGAGAGGGGTGCCCTCGGG 7 0.17500000000000002 No Hit CTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGAT 7 0.17500000000000002 No Hit GAACCTTACCAGGGCTTGACATGCCGCGAATCCTCTTGAAAGAGAGGGGT 7 0.17500000000000002 No Hit TGAACGCTGGCGGCATGCTTAACACATGCAAGTCGAACGGGAAGTGGTGT 7 0.17500000000000002 No Hit GTTTGTTTGATGGTACGTGCTACTCGGATAACCGTAGTAATTCTAGAGCT 7 0.17500000000000002 No Hit AGTGACCAGGCCCGGGCGACTGTTTACCAAAAACACAGGTCTCCGCAAAG 7 0.17500000000000002 No Hit ACCGTGCTGAGCTCCCAAGCAGTGGGAGGGGAAAGTGATCTCTGACCGCG 6 0.15 No Hit GTTCTGGGCCGCACGCGCGCTACACTGATGTATTCAACGAGTATATAGCC 6 0.15 No Hit AGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGATCT 6 0.15 No Hit GATGAACGCTGGCGGCATGCTTAACACATGCAAGTCGAACGGGAAGTGGT 6 0.15 No Hit CTGACACTGAGAGACGAAAGCTAGGGGAGCAAATGGGATTAGAGACCCCA 6 0.15 No Hit ATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCA 6 0.15 No Hit GGGAAGTGGTGTTTCCAGTGGCGAACGGGTGAGTAACGCGTAAGAACCTG 6 0.15 No Hit CAGTGACCAGGCCCGGGCGACTGTTTACCAAAAACACAGGTCTCCGCAAA 6 0.15 No Hit CAGGTCCAGACATAGCAAGGATTGACAGACTGAGAGCTCTTTCTTGATTC 6 0.15 No Hit CGCGAATCCTCTTGAAAGAGAGGGGTGCCCTCGGGAACGCGGACACAGGT 6 0.15 No Hit GGATGATCAGCCACACTGGGACTGAGACACGGCCCAGACTCCTACGGGAG 6 0.15 No Hit CGATCCTGGCTCAGGATGAACGCTGGCGGCATGCTTAACACATGCAAGTC 6 0.15 No Hit GTTTGATCAGGGAAACTACTGAAAATGAATCTGCTAATGAGGGTTACAAA 6 0.15 No Hit GTCTGTGATGCCCTTAGATGTTCTGGGCCGCACGCGCGCTACACTGATGT 6 0.15 No Hit GGAATTTCCGGTGGAGCGGTGAAATGCATTGAGATCGGAAAGAACACCAA 5 0.125 No Hit GGGAAAGTGATCTCTGACCGCGTGCCTGTTGAAGAATGAGCCGGCGACTC 5 0.125 No Hit GGTCGCTTCTGCAACTGGATAACTAGCACTGAAAATCGTCTTTACATCGG 5 0.125 No Hit TTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAA 5 0.125 No Hit GGAAACTACTGAAAATGAATCTGCTAATGAGGGTTACAAATTTGGTCAAG 5 0.125 No Hit GTTAAGTCTCGCAACGAGCGCAACCCTCGTGTTTAGTTGCCACTATGAGT 5 0.125 No Hit GCTAGCTTCTTAGAGGGACTATCGCCGTTTAGGCGACGGAAGTTTGAGGC 5 0.125 No Hit GCCTGACGGAGCAATGCCGCGTGGAGGTGGAAGGCCTACGGGTCGTCAAC 5 0.125 No Hit GTGAAACCGTGCTGAGCTCCCAAGCAGTGGGAGGGGAAAGTGATCTCTGA 5 0.125 No Hit GGGAAACTACTGAAAATGAATCTGCTAATGAGGGTTACAAATTTGGTCAA 5 0.125 No Hit GCGGGACTACCCGCTGAGTTTAAGCATATAAATAAGCGGAGGAGAAGAAA 5 0.125 No Hit GGGAGCTTGACTGCAAGACTCACCCGTCGAGCAGAGACGAAAGTCGGCCT 5 0.125 No Hit GGCTTGACATGCCGCGAATCCTCTTGAAAGAGAGGGGTGCCCTCGGGAAC 5 0.125 No Hit GTTGAAAATAAGCATAGATCCGGAGATTCCCAAATAGGTCAACCTTTTAA 5 0.125 No Hit ACCAGGTCCAGACATAGCAAGGATTGACAGACTGAGAGCTCTTTCTTGAT 5 0.125 No Hit CTGAGGAATAAGCATCGGCTAACTCTGTGCCAGCAGCCGCGGTAAGACAG 5 0.125 No Hit CTCGACGGATCGCACGGCCCTCGTGCCGGCGACGCATCATTCAAATTTCT 5 0.125 No Hit GTTATTTTGGCCGATTAATCTTCCAATATGCTAGTTTCAACAACTCTCGT 5 0.125 No Hit CTCGGGAACGCGGACACAGGTGGTGCATGGCTGTCGTCAGCTCGTGCCGT 5 0.125 No Hit GTTTTGATGATCCCTACCTTATTGACCGCAACTTCTGTATTTATTATCGC 5 0.125 No Hit CCTATTCAGTGCTATGCATGGTTCCTTGGTAACCTCTAGTTTGATCAGGG 5 0.125 No Hit AGCGATTTGTCTGGTTAATTCCGTTAACGAACGAGACCTCAGCCTGCTAA 5 0.125 No Hit ATTCAGTGCTATGCATGGTTCCTTGGTAACCTCTAGTTTGATCAGGGAAA 5 0.125 No Hit CAGGATGAACGCTGGCGGCATGCTTAACACATGCAAGTCGAACGGGAAGT 5 0.125 No Hit GCTGCGACTGCTGTTTTCTTGATTTACCCTATTGGTCAAGGAAGCTTCTC 5 0.125 No Hit TGGTTATACAATGGTGGTCCTTATGAGCTAATTGTTCTACACTTCTTACT 5 0.125 No Hit GTTCAACCTAAATGGATTCAATTTCAACCAATCTGTAGTTGATAGTCAAG 5 0.125 No Hit GTGAAATACCACTACTTTTAACGTTATTTTACTTATTCCGTGGGTCGGAA 5 0.125 No Hit CACAAGGTGACCCTGCTTTTTCAGGGTAAGAAGGGGTAGAGAAAATGCCT 5 0.125 No Hit GTCAAATCCCAGGGCTCAACCCTGGACAGGCGGTGGAAACTACCAAGCTG 5 0.125 No Hit GTCGCGATCTCGCGAGGGTGAGCTAACTCCAAAAACCCGTCCTCAGTTCG 5 0.125 No Hit GCCCGGATCACCAGCTAAGGCCCCTAAATGACCGCTCAGTGATAAAGGAG 5 0.125 No Hit GAACAGACCGCCGGTGTTAAGCCGGAGGAAGGAGAGGATGAGGCCAAGTC 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0125 0.0 0.0 0.0 0.0 68-69 0.025 0.0 0.0 0.0 0.0 70-71 0.05 0.0 0.0 0.0 0.0 72-73 0.05 0.0 0.0 0.0 0.0 74-75 0.05 0.0 0.0 0.0 0.0 76-77 0.0625 0.0 0.0 0.0 0.0 78-79 0.125 0.0 0.0 0.0 0.0 80-81 0.15 0.0 0.0 0.0 0.0 82-83 0.2 0.0 0.0 0.0 0.0 84-85 0.25 0.0 0.0 0.0 0.0 86-87 0.4375 0.0 0.0 0.0 0.0 88-89 0.55 0.0 0.0 0.0 0.0 90-91 0.7125 0.0 0.0 0.0 0.0 92-93 0.8875 0.0 0.0 0.0 0.0 94-95 0.975 0.0 0.0 0.0 0.0 96-97 1.1625 0.0 0.0 0.0 0.0 98-99 1.4125 0.0 0.0 0.0 0.0 100-101 1.75 0.0 0.0 0.0 0.0 102-103 2.075 0.0 0.0 0.0 0.0 104-105 2.425 0.0 0.0 0.0 0.0 106-107 2.7625 0.0 0.0 0.0 0.0 108-109 3.1125 0.0 0.0 0.0 0.0 110-111 3.7125 0.0 0.0 0.0 0.0 112-113 4.199999999999999 0.0 0.0 0.0 0.0 114-115 4.675000000000001 0.0 0.0 0.0 0.0 116-117 5.15 0.0 0.0 0.0 0.0 118-119 5.7 0.0 0.0 0.0 0.0 120-121 6.2875 0.0 0.0 0.0 0.0 122-123 6.800000000000001 0.0 0.0 0.0 0.0 124-125 7.5375 0.0 0.0 0.0 0.0 126-127 8.2375 0.0 0.0 0.0 0.0 128-129 8.8 0.0 0.0 0.0 0.0 130-131 9.55 0.0 0.0 0.0 0.0 132-133 10.0875 0.0 0.0 0.0 0.0 134-135 10.875 0.0 0.0 0.0 0.0 136-137 11.6125 0.0 0.0 0.0 0.0 138-139 12.325 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGTTCGC 10 0.0068449317 144.90001 145 >>END_MODULE Read 966834 spots for SRR6941605.sra Written 966834 spots for SRR6941605.sra Read 966834 spots for SRR6941605.sra Written 966834 spots for SRR6941605.sra Read 966834 spots for SRR6941605.sra Written 966834 spots for SRR6941605.sra Read 966834 spots for SRR6941605.sra Written 966834 spots for SRR6941605.sra Read 966834 spots for SRR6941605.sra Written 966834 spots for SRR6941605.sra Read 966834 spots for SRR6941605.sra Written 966834 spots for SRR6941605.sra Read 966834 spots for SRR6941605.sra Written 966834 spots for SRR6941605.sra Read 966834 spots for SRR6941605.sra Written 966834 spots for SRR6941605.sra Read 966834 spots for SRR6941605.sra Written 966834 spots for SRR6941605.sra Read 966834 spots for SRR6941605.sra Written 966834 spots for SRR6941605.sra Read 966834 spots for SRR6941605.sra Written 966834 spots for SRR6941605.sra Read 966849 spots for SRR6941605.sra Written 966849 spots for SRR6941605.sra Read 966834 spots for SRR6941605.sra Written 966834 spots for SRR6941605.sra Read 966834 spots for SRR6941605.sra Written 966834 spots for SRR6941605.sra Read 966834 spots for SRR6941605.sra Written 966834 spots for SRR6941605.sra Read 966834 spots for SRR6941605.sra Written 966834 spots for SRR6941605.sra Read 966834 spots for SRR6941605.sra Written 966834 spots for SRR6941605.sra Read 966834 spots for SRR6941605.sra Written 966834 spots for SRR6941605.sra Read 966834 spots for SRR6941605.sra Written 966834 spots for SRR6941605.sra Read 966834 spots for SRR6941605.sra Written 966834 spots for SRR6941605.sra SRR ids: ['SRR6941605.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_3sr_n4sw SRR6941605.sra spots: 19336695 blocks: [[1, 966834], [966835, 1933668], [1933669, 2900502], [2900503, 3867336], [3867337, 4834170], [4834171, 5801004], [5801005, 6767838], [6767839, 7734672], [7734673, 8701506], [8701507, 9668340], [9668341, 10635174], [10635175, 11602008], [11602009, 12568842], [12568843, 13535676], [13535677, 14502510], [14502511, 15469344], [15469345, 16436178], [16436179, 17403012], [17403013, 18369846], [18369847, 19336695]] SRR6941605 file size 6530871 SRR6941605 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR6941605 SRR6941605_1.fastq SRR6941605_2.fastq Input file: SRR6941605_1.fastq Paired file: SRR6941605_2.fastq trimmed: SRR6941605-trimmed-pair1.fastq, SRR6941605-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Fri Dec 6 12:55:47 2024 >> started Fri Dec 6 12:56:11 2024 >> done (24.501s) 19336695 read pairs processed; of these: 8882 ( 0.05%) short read pairs filtered out after trimming by size control 9625 ( 0.05%) empty read pairs filtered out after trimming by size control 19318188 (99.90%) read pairs available; of these: 8985174 (46.51%) trimmed read pairs available after processing 10333014 (53.49%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 6 0.00% 19 5 0.00% 20 3 0.00% 21 3 0.00% 22 5 0.00% 23 3 0.00% 24 7 0.00% 25 5 0.00% 26 5 0.00% 27 12 0.00% 28 3 0.00% 29 6 0.00% 30 6 0.00% 31 13 0.00% 32 13 0.00% 33 16 0.00% 34 13 0.00% 35 18 0.00% 36 12 0.00% 37 24 0.00% 38 19 0.00% 39 19 0.00% 40 28 0.00% 41 35 0.00% 42 33 0.00% 43 50 0.00% 44 35 0.00% 45 47 0.00% 46 49 0.00% 47 65 0.00% 48 98 0.00% 49 87 0.00% 50 121 0.00% 51 137 0.00% 52 177 0.00% 53 162 0.00% 54 215 0.00% 55 235 0.00% 56 289 0.00% 57 286 0.00% 58 286 0.00% 59 394 0.00% 60 413 0.00% 61 583 0.00% 62 688 0.00% 63 769 0.00% 64 860 0.00% 65 929 0.00% 66 1047 0.01% 67 1235 0.01% 68 1358 0.01% 69 1571 0.01% 70 1882 0.01% 71 2029 0.01% 72 2521 0.01% 73 2769 0.01% 74 2985 0.02% 75 3390 0.02% 76 3915 0.02% 77 4222 0.02% 78 4716 0.02% 79 5663 0.03% 80 6503 0.03% 81 7012 0.04% 82 7849 0.04% 83 8708 0.05% 84 9443 0.05% 85 11718 0.06% 86 12270 0.06% 87 13043 0.07% 88 14875 0.08% 89 15291 0.08% 90 16407 0.08% 91 17827 0.09% 92 20370 0.11% 93 21474 0.11% 94 22021 0.11% 95 25082 0.13% 96 24638 0.13% 97 27382 0.14% 98 28439 0.15% 99 30330 0.16% 100 31512 0.16% 101 33974 0.18% 102 33261 0.17% 103 34470 0.18% 104 36125 0.19% 105 37047 0.19% 106 38615 0.20% 107 40323 0.21% 108 42945 0.22% 109 44344 0.23% 110 44392 0.23% 111 45562 0.24% 112 47611 0.25% 113 47351 0.25% 114 49727 0.26% 115 53861 0.28% 116 55009 0.28% 117 52391 0.27% 118 54267 0.28% 119 53877 0.28% 120 58160 0.30% 121 60138 0.31% 122 62770 0.32% 123 66697 0.35% 124 64888 0.34% 125 70636 0.37% 126 68344 0.35% 127 70570 0.37% 128 68843 0.36% 129 72541 0.38% 130 70425 0.36% 131 74096 0.38% 132 75449 0.39% 133 76926 0.40% 134 79708 0.41% 135 80212 0.42% 136 84493 0.44% 137 85046 0.44% 138 91830 0.48% 139 96070 0.50% 140 97245 0.50% 141 107630 0.56% 142 112292 0.58% 143 120434 0.62% 144 136255 0.71% 145 159193 0.82% 146 186028 0.96% 147 236294 1.22% 148 340200 1.76% 149 659463 3.41% 150 4088362 21.16% 151 10333014 53.49% 19318188 reads passed initial QC criterion=sequence-density sequence-density=3.20 sequence-density-rank=1 fanout-score=1.92 fanout-score-rank=32 prefix-density=3.16 prefix-fanout=1.9 sequence=GTATTTAGCCTTG criterion=fanout-score sequence-density=0.01 sequence-density-rank=37 fanout-score=303.61 fanout-score-rank=1 prefix-density=1.52 prefix-fanout=1.0 sequence=GTAGAACAAGATATTGGGTATTTCTCGCTTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCATTAATAGCGGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTACGTTCGTGCATTACTTCCATACCAAGATTAGCACGGTTGATGATATCAGCCCAAGTATTAATAACGCGACCTTGACTATCAACTACAGATTGGTTGAAATTGAATCCATTTAGGTTGAACGCCATAGTACTAATACCTAAAGCAGTGAACCAGATTCCTACTACAGGCCAAGCAGCCAAGAAGAAGTGTAAAGAACGAGAGTTGTTGAAACTAGCATATTGGAAGATTAATCGGCCAAAATAACCATGAGCAGCCACAATATTATAAGTCTCTTCCTCTTGACCAAATTTGTAACCCTCATTAGCAGATTCATTTTCAGTAGTTTCCCTGATCAAACTAGAGGTTACCAAGGAACCATGCATAGCACTGAATAGGGAACCG criterion=sequence-density sequence-density=1.61 sequence-density-rank=1 fanout-score=2.38 fanout-score-rank=28 prefix-density=1.92 prefix-fanout=2.0 sequence=CCGAAAGATGGT criterion=fanout-score sequence-density=0.02 sequence-density-rank=33 fanout-score=80.59 fanout-score-rank=1 prefix-density=1.10 prefix-fanout=1.1 sequence=AGAAGGGGTGCCCCCTCACAAAAGGGGGTCGCAGTGACCAGGCCCGGGCGACTGTTTACCAAAAACACAGGTCTCCGCAAAGTCGTAAGACCATGTATGGGGGCTGACGCCTGCCCAGTGCCGGAAGGTCAAGGAAGTTGGTGAACTGATGACAGGGAAGCCGGCGACCGAAGCCCCGGTGAACGGCGGCCGTAAC Potential 3prime adapter identified. Now checking if in reference sequence /dee2/code/volunteer_pipeline.sh: line 905: -f: command not found /dee2/code/volunteer_pipeline.sh: line 906: -f: command not found Adapter seq not found in reference. Now shuffling file before clipping skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -t 20 -x GTATTTAGCCTTG -y CCGAAAGATGGT -o SRR6941605 SRR6941605_1.fastq SRR6941605_2.fastq Input file: SRR6941605_1.fastq Paired file: SRR6941605_2.fastq trimmed: SRR6941605-trimmed-pair1.fastq, SRR6941605-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): GTATTTAGCCTTG -- paired 3' end adapter sequence (-y): CCGAAAGATGGT -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Fri Dec 6 12:57:37 2024 >> started Fri Dec 6 12:57:43 2024 >> done (6.323s) 6439396 read pairs processed; of these: 467 ( 0.01%) short read pairs filtered out after trimming by size control 2457 ( 0.04%) empty read pairs filtered out after trimming by size control 6436472 (99.95%) read pairs available; of these: 950 ( 0.01%) trimmed read pairs available after processing 6435522 (99.99%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 2 0.00% 19 2 0.00% 20 2 0.00% 21 2 0.00% 22 1 0.00% 23 2 0.00% 24 1 0.00% 25 2 0.00% 26 0 0.00% 27 8 0.00% 28 1 0.00% 29 2 0.00% 30 2 0.00% 31 4 0.00% 32 3 0.00% 33 8 0.00% 34 4 0.00% 35 5 0.00% 36 5 0.00% 37 13 0.00% 38 5 0.00% 39 9 0.00% 40 7 0.00% 41 11 0.00% 42 14 0.00% 43 22 0.00% 44 9 0.00% 45 14 0.00% 46 15 0.00% 47 19 0.00% 48 34 0.00% 49 30 0.00% 50 36 0.00% 51 40 0.00% 52 56 0.00% 53 50 0.00% 54 77 0.00% 55 82 0.00% 56 93 0.00% 57 87 0.00% 58 95 0.00% 59 118 0.00% 60 127 0.00% 61 198 0.00% 62 221 0.00% 63 255 0.00% 64 310 0.00% 65 306 0.00% 66 355 0.01% 67 410 0.01% 68 450 0.01% 69 514 0.01% 70 635 0.01% 71 684 0.01% 72 828 0.01% 73 923 0.01% 74 996 0.02% 75 1185 0.02% 76 1286 0.02% 77 1413 0.02% 78 1587 0.02% 79 1883 0.03% 80 2226 0.03% 81 2336 0.04% 82 2620 0.04% 83 2943 0.05% 84 3157 0.05% 85 3818 0.06% 86 4190 0.07% 87 4379 0.07% 88 4946 0.08% 89 5020 0.08% 90 5480 0.09% 91 6056 0.09% 92 6727 0.10% 93 7279 0.11% 94 7275 0.11% 95 8304 0.13% 96 8183 0.13% 97 9146 0.14% 98 9498 0.15% 99 9995 0.16% 100 10513 0.16% 101 11493 0.18% 102 10962 0.17% 103 11420 0.18% 104 11999 0.19% 105 12337 0.19% 106 12785 0.20% 107 13393 0.21% 108 14329 0.22% 109 14891 0.23% 110 14834 0.23% 111 15218 0.24% 112 16023 0.25% 113 15747 0.24% 114 16663 0.26% 115 17829 0.28% 116 18315 0.28% 117 17444 0.27% 118 18080 0.28% 119 17892 0.28% 120 19365 0.30% 121 20077 0.31% 122 20992 0.33% 123 22339 0.35% 124 21636 0.34% 125 23433 0.36% 126 22555 0.35% 127 23389 0.36% 128 22739 0.35% 129 24301 0.38% 130 23412 0.36% 131 24706 0.38% 132 24952 0.39% 133 25672 0.40% 134 26540 0.41% 135 26861 0.42% 136 28165 0.44% 137 28196 0.44% 138 30769 0.48% 139 31903 0.50% 140 32351 0.50% 141 36018 0.56% 142 37630 0.58% 143 40036 0.62% 144 45334 0.70% 145 52725 0.82% 146 62331 0.97% 147 78623 1.22% 148 113477 1.76% 149 219785 3.41% 150 1361795 21.16% 151 3443062 53.49% criterion=sequence-density sequence-density=3.18 sequence-density-rank=1 fanout-score=1.92 fanout-score-rank=31 prefix-density=3.16 prefix-fanout=1.9 sequence=GTATTTAGCCTTG criterion=fanout-score sequence-density=0.01 sequence-density-rank=38 fanout-score=291.49 fanout-score-rank=1 prefix-density=1.52 prefix-fanout=1.0 sequence=GTAGAACAAGATATTGGGTATTTCTCGCTTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCATTAATAGCGGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTACGTTCGTGCATTACTTCCATACCAAGATTAGCACGGTTGATGATATCAGCCCAAGTATTAATAACGCGACCTTGACTATCAACTACAGATTGGTTGAAATTGAATCCATTTAGGTTGAACGCCATAGTACTAATACCTAAAGCAGTGAACCAGATTCCTACTACAGGCCAAGCAGCCAAGAAGAAGTGTAAAGAACGAGAGTTGTTGAAACTAGCATATTGGAAGATTAATCGGCCAAAATAACCATGAGCAGCCACAATATTATAAGTCTCTTCCTCTTGACCAAATTTGTAACCCTCATTAGCAGATTCATTTTCAGTAGTTTCCCTGATCAAACTAGAGGTTACCAAGGAACCATGCATAGCACTGAATAGGGAACCG criterion=sequence-density sequence-density=1.56 sequence-density-rank=1 fanout-score=2.41 fanout-score-rank=29 prefix-density=1.89 prefix-fanout=2.0 sequence=CCGAAAGATGGT criterion=fanout-score sequence-density=0.02 sequence-density-rank=32 fanout-score=74.86 fanout-score-rank=1 prefix-density=1.09 prefix-fanout=1.1 sequence=AGAAGGGGTGCCCCCTCACAAAAGGGGGTCGCAGTGACCAGGCCCGGGCGACTGTTTACCAAAAACACAGGTCTCCGCAAAGTCGTAAGACCATGTATGGGGGCTGACGCCTGCCCAGTGCCGGAAGGTCAAGGAAGTTGGTGAACTGATGACAGGGAAGCCGGCGACCGAAGCCCCGGTGAACGGCGGCCGTAAC SRR6941605 testing PE reads STAR mapping to Ensembl genome Started job on | Dec 06 12:58:27 Started mapping on | Dec 06 12:58:27 Finished on | Dec 06 13:00:09 Mapping speed, Million of reads per hour | 681.72 Number of input reads | 19315264 Average input read length | 291 UNIQUE READS: Uniquely mapped reads number | 8005573 Uniquely mapped reads % | 41.45% Average mapped length | 294.89 Number of splices: Total | 974806 Number of splices: Annotated (sjdb) | 872797 Number of splices: GT/AG | 924479 Number of splices: GC/AG | 11907 Number of splices: AT/AC | 2783 Number of splices: Non-canonical | 35637 Mismatch rate per base, % | 0.14% Deletion rate per base | 0.01% Deletion average length | 1.60 Insertion rate per base | 0.01% Insertion average length | 1.66 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 6462881 % of reads mapped to multiple loci | 33.46% Number of reads mapped to too many loci | 698801 % of reads mapped to too many loci | 3.62% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 3.05% % of reads unmapped: other | 18.43% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 4850780 4850780 4850780 N_multimapping 6462881 6462881 6462881 N_noFeature 5298016 7856005 5363148 N_ambiguous 183820 1877 102260 UnstrandedReadsAssigned:2523737 PositiveStrandReadsAssigned:147691 NegativeStrandReadsAssigned:2540165 Dataset is classified negative stranded MeadianReadLen=151 20thPercentileLength=149 echo kmer=145 SRR6941605 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,972 [index] number of k-mers: 66,720,672 [index] number of equivalence classes: 111,837 [quant] running in paired-end mode [quant] will process pair 1: SRR6941605-trimmed-pair1.fastq SRR6941605-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 19,315,264 reads, 5,023,805 reads pseudoaligned [quant] estimated average fragment length: 205.746 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 996 rounds 52973 SRR6941605.ke.tsv 35125 SRR6941605.se.tsv 88098 total ==> SRR6941605.ke.tsv <== target_id length eff_length est_counts tpm PNS24245 936 731.625 6.90974 0.96827 PNS24247 1044 839.254 2.59922 0.317521 PNS24249 1928 1723.25 2.23966 0.133246 PNS24246 1044 839.254 2.59922 0.317521 PNS24248 1044 839.254 2.59922 0.317521 PNS24244 1471 1266.25 4.05296 0.328151 PNS24243 293 115.099 0 0 KQK14069 1603 1398.25 802.974 58.876 KQK14071 474 277.202 24.4271 9.03438 ==> SRR6941605.se.tsv <== BRADI_1g14170v3 1040 BRADI_1g53295v3 9 BRADI_1g59795v3 27 BRADI_1g07683v3 0 BRADI_1g00485v3 0 BRADI_1g20270v3 14 BRADI_1g74790v3 2 BRADI_1g09890v3 0 BRADI_1g77505v3 9 BRADI_1g48960v3 0 SRR6941605 completed mapping pipeline successfully