Starting /dee2/code/volunteer_pipeline.sh SRR6941606 current disk space = 1551184781312 free memory = 1602324156 SRR6941606 SRAfilesize c54b9791da1a16ecdcf3af1aa1c7de75 SRR6941606.sra SRR6941606.sra file validated SRR6941606 is paired end SRR6941606 is conventional basespace SRR6941606 read1 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR6941606_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.66075 34.0 33.0 34.0 32.0 34.0 2 33.08725 34.0 33.0 34.0 32.0 34.0 3 33.199 34.0 33.0 34.0 32.0 34.0 4 33.388 34.0 33.0 34.0 33.0 34.0 5 33.3855 34.0 33.0 34.0 33.0 34.0 6 37.2495 38.0 38.0 38.0 36.0 38.0 7 37.48725 38.0 38.0 38.0 37.0 38.0 8 37.59925 38.0 38.0 38.0 38.0 38.0 9 37.602 38.0 38.0 38.0 38.0 38.0 10-14 37.63735 38.0 38.0 38.0 38.0 38.0 15-19 37.6178 38.0 38.0 38.0 38.0 38.0 20-24 37.62475 38.0 38.0 38.0 38.0 38.0 25-29 37.52235 38.0 38.0 38.0 38.0 38.0 30-34 37.4302 38.0 38.0 38.0 37.8 38.0 35-39 37.494949999999996 38.0 38.0 38.0 37.8 38.0 40-44 37.4998 38.0 38.0 38.0 38.0 38.0 45-49 37.513099999999994 38.0 38.0 38.0 38.0 38.0 50-54 37.475350000000006 38.0 38.0 38.0 38.0 38.0 55-59 37.464749999999995 38.0 38.0 38.0 38.0 38.0 60-64 37.4503 38.0 38.0 38.0 37.4 38.0 65-69 37.31605 38.0 38.0 38.0 37.0 38.0 70-74 37.3192 38.0 38.0 38.0 37.0 38.0 75-79 37.350849999999994 38.0 38.0 38.0 37.0 38.0 80-84 37.31785 38.0 38.0 38.0 37.0 38.0 85-89 37.097699999999996 38.0 38.0 38.0 36.0 38.0 90-94 37.140699999999995 38.0 38.0 38.0 36.0 38.0 95-99 37.01115 38.0 38.0 38.0 35.8 38.0 100-104 37.06245 38.0 38.0 38.0 36.0 38.0 105-109 36.87660000000001 38.0 38.0 38.0 35.2 38.0 110-114 36.521699999999996 38.0 38.0 38.0 34.2 38.0 115-119 36.4842 38.0 38.0 38.0 34.0 38.0 120-124 36.544650000000004 38.0 38.0 38.0 34.0 38.0 125-129 36.60039999999999 38.0 38.0 38.0 34.4 38.0 130-134 36.40325 38.0 38.0 38.0 34.0 38.0 135-139 36.112399999999994 38.0 37.8 38.0 33.2 38.0 140-144 36.007600000000004 38.0 37.2 38.0 32.8 38.0 145-149 35.59705 38.0 36.2 38.0 31.2 38.0 150-151 31.555375 35.5 31.0 38.0 16.5 38.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 1.0 8 0.0 9 0.0 10 1.0 11 0.0 12 0.0 13 0.0 14 1.0 15 1.0 16 0.0 17 0.0 18 0.0 19 0.0 20 2.0 21 1.0 22 4.0 23 4.0 24 5.0 25 8.0 26 7.0 27 13.0 28 18.0 29 18.0 30 22.0 31 36.0 32 58.0 33 76.0 34 95.0 35 180.0 36 412.0 37 3037.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 42.653653389606966 12.134001582695857 6.752835663413348 38.459509364283825 2 24.081020255063766 14.453613403350838 31.532883220805203 29.932483120780194 3 19.825 20.424999999999997 26.0 33.75 4 25.6 27.250000000000004 22.825 24.325 5 24.525 32.5 22.325 20.65 6 19.650000000000002 32.875 24.125 23.35 7 16.025 23.425 40.875 19.675 8 18.075 20.8 30.15 30.975 9 19.55 19.8 32.9 27.750000000000004 10-14 21.725 26.775 24.48 27.02 15-19 22.25 25.25 26.325 26.174999999999997 20-24 22.295 26.0 25.56 26.145000000000003 25-29 22.695 25.185000000000002 25.990000000000002 26.13 30-34 22.115000000000002 26.205000000000002 25.324999999999996 26.355 35-39 22.007200720072007 25.782578257825783 26.33763376337634 25.872587258725872 40-44 22.103841536614645 25.185074029611844 25.885354141656663 26.825730292116845 45-49 21.17 25.374999999999996 27.565 25.89 50-54 21.968787515006003 25.70528211284514 25.85534213685474 26.47058823529412 55-59 21.934386877375474 25.440088017603518 26.300260052010405 26.325265053010604 60-64 22.048307246086914 25.148772315847378 26.413962094314147 26.38895834375156 65-69 22.595000000000002 25.855 24.825 26.724999999999998 70-74 23.251162558127906 25.27126356317816 24.671233561678083 26.806340317015852 75-79 21.990000000000002 26.165 25.435000000000002 26.41 80-84 23.15231523152315 25.86758675867587 25.082508250825082 25.897589758975897 85-89 22.215 24.805 25.869999999999997 27.11 90-94 22.842284228422844 26.292629262926294 24.64246424642464 26.222622262226224 95-99 21.884999999999998 25.814999999999998 25.45 26.85 100-104 22.27891156462585 25.820328131252502 25.53021208483393 26.370548219287716 105-109 22.705000000000002 24.965 26.040000000000003 26.290000000000003 110-114 22.851121481258467 25.29981434091023 26.01736163379999 25.83170254403131 115-119 22.056318268363565 26.355346227076858 25.002505261048203 26.58583024351137 120-124 22.013711654906672 26.642646249311912 23.46494520342291 27.878696892358505 125-129 22.4706176544136 26.62165541385346 23.775943985996502 27.131782945736433 130-134 23.058458768815324 26.934040106015907 23.793569035355304 26.213932089813476 135-139 23.669999999999998 25.695 24.435000000000002 26.200000000000003 140-144 23.035 26.665 24.665 25.635 145-149 22.43 26.3 24.709999999999997 26.56 150-151 19.85 27.400000000000002 24.2 28.549999999999997 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1.0 1 0.5 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 1.0 20 2.0 21 2.0 22 1.5 23 1.0 24 1.5 25 2.5 26 5.5 27 7.5 28 9.0 29 12.0 30 15.5 31 16.0 32 19.5 33 24.5 34 17.5 35 22.5 36 62.5 37 129.0 38 137.5 39 103.0 40 133.0 41 155.5 42 137.5 43 140.5 44 141.0 45 141.5 46 125.0 47 92.5 48 102.0 49 101.5 50 111.0 51 146.0 52 142.0 53 152.5 54 193.5 55 235.5 56 257.0 57 212.0 58 170.0 59 147.5 60 109.5 61 77.0 62 49.0 63 38.5 64 28.5 65 14.0 66 8.0 67 8.5 68 7.0 69 5.0 70 5.0 71 4.5 72 4.5 73 3.0 74 1.0 75 0.5 76 2.0 77 2.5 78 1.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content warn #Base N-Count 1 5.225 2 0.025 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.01 40-44 0.04 45-49 0.0 50-54 0.04 55-59 0.02 60-64 0.015 65-69 0.0 70-74 0.005 75-79 0.0 80-84 0.01 85-89 0.0 90-94 0.01 95-99 0.0 100-104 0.04 105-109 0.0 110-114 0.35500000000000004 115-119 0.21 120-124 0.08499999999999999 125-129 0.025 130-134 0.015 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 69.6 #Duplication Level Percentage of deduplicated Percentage of total 1 80.81896551724138 56.25 2 10.272988505747128 14.299999999999999 3 3.8433908045977008 8.025 4 2.119252873563218 5.8999999999999995 5 1.113505747126437 3.875 6 0.646551724137931 2.7 7 0.3232758620689655 1.575 8 0.17959770114942528 1.0 9 0.17959770114942528 1.125 >10 0.5028735632183908 5.25 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCATTAAT 36 0.8999999999999999 No Hit GTACAAGGCCCGGGAACGGATTCACCGCCGTATGGCTGACCGGCGATTAC 21 0.525 No Hit GTCGGTTTCGGGTACAGGTACCCTTTTGTTGAAGGTCGTTCGAGCTTTTC 18 0.44999999999999996 No Hit GGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTACGTT 16 0.4 No Hit GCGGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTACG 14 0.35000000000000003 No Hit GCCAAAATAACCATGAGCAGCCACAATATTATAAGTCTCTTCCTCTTGAC 14 0.35000000000000003 No Hit GTTGAAACTAGCATATTGGAAGATTAATCGGCCAAAATAACCATGAGCAG 14 0.35000000000000003 No Hit CTTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCAT 13 0.325 No Hit GCGGAAAAACCTTATCCATTAATAGCGGGAACTTCAAGAGCAGCTAGATC 12 0.3 No Hit CCTAGATGTCCAGTCAACTGCTGCGCCTCAACGCATTTCGGGGAGAACCA 11 0.27499999999999997 No Hit CCTCACGGTACTACTTCGCTATCGGTCACCCAGGAGTATTTAGCCTTGCA 11 0.27499999999999997 No Hit CGCAGAAGTAGGAATAATAGCACCAGAGATAATATTGTTTCCATAAAGTA 10 0.25 No Hit GGCATGATGACTTGGCCTCATCCTCTCCTTCCTCCGGCTTAACACCGGCG 10 0.25 No Hit CTCCACGCGGCATTGCTCCGTCAGGCTTTCGCCCATTGCGGAAAATTCCC 10 0.25 No Hit GCTCCACCGCTTGTGCGGGCCCCCGTCAATTCCTTTGAGTTTCATTCTTG 9 0.22499999999999998 No Hit CCCTACCGTACTCCAGCTTGGTAGTTTCCACCGCCTGTCCAGGGTTGAGC 9 0.22499999999999998 No Hit CCACATGCTCCACCGCTTGTGCGGGCCCCCGTCAATTCCTTTGAGTTTCA 9 0.22499999999999998 No Hit GGGTGGGCTTACTACTTATATGCTTTCAGCAGTTATCCTCTCCGCACTTG 9 0.22499999999999998 No Hit CCGGGAACGGATTCACCGCCGTATGGCTGACCGGCGATTACTAGCGATTC 9 0.22499999999999998 No Hit GCCGAAAACACCAGCTACACCTAACATGTGAAATGGATGCATAAGGATGT 8 0.2 No Hit GTCCTCTCAATGCTCTAACGCCCACACCGGATATGGACCGAACTGTCTCA 8 0.2 No Hit GGGCTTACTACTTATATGCTTTCAGCAGTTATCCTCTCCGCACTTGGCTA 8 0.2 No Hit GTCGGTTCGGACCTCTGCTTAGTTTCATCCAAGCTTCATCCTGGTCATGG 8 0.2 No Hit CCTGTGTCGGTTTCGGGTACAGGTACCCTTTTGTTGAAGGTCGTTCGAGC 8 0.2 No Hit CGGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTACGT 7 0.17500000000000002 No Hit CTAATACCTAAAGCAGTGAACCAGATTCCTACTACAGGCCAAGCAGCCAA 7 0.17500000000000002 No Hit GTGTACAAGGCCCGGGAACGGATTCACCGCCGTATGGCTGACCGGCGATT 7 0.17500000000000002 No Hit GCCAGCTCCTATAGTGTGACGGGCGGTGTGTACAAGGCCCGGGAACGGAT 7 0.17500000000000002 No Hit CTTGGGGTGGGCTTACTACTTATATGCTTTCAGCAGTTATCCTCTCCGCA 7 0.17500000000000002 No Hit GTGTGTACAAGGCCCGGGAACGGATTCACCGCCGTATGGCTGACCGGCGA 7 0.17500000000000002 No Hit GGCTGATCATCCTCTCGGACCAGCTACTGATCATCGCCTTGGTAAGCTAT 7 0.17500000000000002 No Hit GCTTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCA 7 0.17500000000000002 No Hit CTCTGCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTCCTAAAG 7 0.17500000000000002 No Hit GGCCAAAATAACCATGAGCAGCCACAATATTATAAGTCTCTTCCTCTTGA 6 0.15 No Hit CGTCAATTCCTTTGAGTTTCATTCTTGCGAACGTACTCCCCAGGCGGGAT 6 0.15 No Hit GGCGCATACCCAAACGGAAACTAAGTTCCCACTCACGACCCATATAACAA 6 0.15 No Hit GTCGCAGCTGCAACAGGAGCTGAATATGCAACAGCAATCCAAGGGCGCAT 6 0.15 No Hit GTTCTTTTCACCTTTCCCTCACGGTACTACTTCGCTATCGGTCACCCAGG 6 0.15 No Hit ACATGCTCCACCGCTTGTGCGGGCCCCCGTCAATTCCTTTGAGTTTCATT 6 0.15 No Hit GTCGCCCAGGGCATAAGGGGCATGATGACTTGGCCTCATCCTCTCCTTCC 6 0.15 No Hit GCTGCGATGAAGGCGATAATAAATACAGAAGTTGCGGTCAATAAGGTAGG 6 0.15 No Hit CCACAATATTATAAGTCTCTTCCTCTTGACCAAATTTGTAACCCTCATTA 6 0.15 No Hit GCCCAATCATTCCGGATAACGCTTGCATCCTCTGTCTTACCGCGGCTGCT 6 0.15 No Hit GAAAAACCTTATCCATTAATAGCGGGAACTTCAAGAGCAGCTAGATCTAG 6 0.15 No Hit GCATAAGGATGTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAAAGTA 6 0.15 No Hit GGCGGATTTCTCCTTTTGCTCCTCAGCCTACGGGGTATTAGCAACCGTTT 6 0.15 No Hit GGCAATTTCAAGCACTCTTTGACTCTCTTTTCAAAGTCCTTTTCATCTTT 6 0.15 No Hit ATCACGATGAAATTTCCCAAGATTACCCGGGCCTGTCGGCCAAGGCTATA 6 0.15 No Hit GTTTACGGCTAGGACTACTGGGGTCTCTAATCCCATTTGCTCCCCTAGCT 6 0.15 No Hit GACCCATATAACAAGCTACACCAAGTAAGAAGTGTAGAACAATTAGCTCA 6 0.15 No Hit ACCACATGCTCCACCGCTTGTGCGGGCCCCCGTCAATTCCTTTGAGTTTC 6 0.15 No Hit CACCGCTTGTGCGGGCCCCCGTCAATTCCTTTGAGTTTCATTCTTGCGAA 5 0.125 No Hit CAGAGAAGCTTCCTTGACCAATAGGGTAAATCAAGAAAACAGCAGTCGCA 5 0.125 No Hit CAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCATTAATAGCGGGAA 5 0.125 No Hit CCTCAGCCTACGGGGTATTAGCAACCGTTTCCAGTTGTTGTTCCCCTCCC 5 0.125 No Hit GGAAAAACCTTATCCATTAATAGCGGGAACTTCAAGAGCAGCTAGATCTA 5 0.125 No Hit ATGAAGACTCGCTTTCGCTACGGCTCCGGTGGGTTCCGTTCCCTTAACCA 5 0.125 No Hit GGCGATAATAAATACAGAAGTTGCGGTCAATAAGGTAGGGATCATCAAAA 5 0.125 No Hit CAAGTATTAATAACGCGACCTTGACTATCAACTACAGATTGGTTGAAATT 5 0.125 No Hit CGGGCGGTGTGTACAAGGCCCGGGAACGGATTCACCGCCGTATGGCTGAC 5 0.125 No Hit GCCGACTTTCGTCTCTGCTCGACGGGTGAGTCTTGCAGTCAAGCTCCCTT 5 0.125 No Hit GGGAAGTTGTGAGCATTACGTTCGTGCATTACTTCCATACCAAGATTAGC 5 0.125 No Hit GTTCGAGCTTTTCCTGGGAGTATGGCATCGGTTACATACTTCAGTGCCGT 5 0.125 No Hit GCATATTGGAAGATTAATCGGCCAAAATAACCATGAGCAGCCACAATATT 5 0.125 No Hit GGTGGGCTTACTACTTATATGCTTTCAGCAGTTATCCTCTCCGCACTTGG 5 0.125 No Hit GCTAGTTATCCAGTTGCAGAAGCGACCCCACAGGCTTGTACTTTCGCGTC 5 0.125 No Hit TTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCATTAATA 5 0.125 No Hit CGGAAAAACCTTATCCATTAATAGCGGGAACTTCAAGAGCAGCTAGATCT 5 0.125 No Hit GTGCTCTGCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTCCTA 5 0.125 No Hit CTACAGGCCAAGCAGCCAAGAAGAAGTGTAAAGAACGAGAGTTGTTGAAA 5 0.125 No Hit GTTTCTTCTCCGAGAAAAGAAGTTGACGACCCGTAGGCCTTCCACCTCCA 5 0.125 No Hit AGCGGAAAAACCTTATCCATTAATAGCGGGAACTTCAAGAGCAGCTAGAT 5 0.125 No Hit CAGGGTTCCAAACTCATAGTGGCAACTAAACACGAGGGTTGCGCTCGTTG 5 0.125 No Hit GCTTTCTTTTCCTCTGGCTACTAAGATGTTTCAGTTCGCCAGGTTGTCTC 5 0.125 No Hit CATAAGGATGTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAAAGTAC 5 0.125 No Hit TAAAGAACGAGAGTTGTTGAAACTAGCATATTGGAAGATTAATCGGCCAA 5 0.125 No Hit GTGAAATGGATGCATAAGGATGTTGTGCTCTGCCTGGAATACAATCATAA 5 0.125 No Hit CCGGAACCCAAAGACTTTGATTTCTCATAAGGTGCCGGCGGAGTCCTATA 5 0.125 No Hit GCACGTGTGTCGCCCAGGGCATAAGGGGCATGATGACTTGGCCTCATCCT 5 0.125 No Hit CCCCACTGCTGCCTCCCGTAGGAGTCTGGGCCGTGTCTCAGTCCCAGTGT 5 0.125 No Hit GCCGATGCTTATTCCTCAGATACCGTCATTGTTTCTTCTCCGAGAAAAGA 5 0.125 No Hit GGGGCTGCGATGAAGGCGATAATAAATACAGAAGTTGCGGTCAATAAGGT 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0125 0.0 0.0 0.0 0.0 54-55 0.025 0.0 0.0 0.0 0.0 56-57 0.025 0.0 0.0 0.0 0.0 58-59 0.037500000000000006 0.0 0.0 0.0 0.0 60-61 0.05 0.0 0.0 0.0 0.0 62-63 0.05 0.0 0.0 0.0 0.0 64-65 0.05 0.0 0.0 0.0 0.0 66-67 0.05 0.0 0.0 0.0 0.0 68-69 0.05 0.0 0.0 0.0 0.0 70-71 0.05 0.0 0.0 0.0 0.0 72-73 0.07500000000000001 0.0 0.0 0.0 0.0 74-75 0.125 0.0 0.0 0.0 0.0 76-77 0.125 0.0 0.0 0.0 0.0 78-79 0.125 0.0 0.0 0.0 0.0 80-81 0.1375 0.0 0.0 0.0 0.0 82-83 0.175 0.0 0.0 0.0 0.0 84-85 0.3375 0.0 0.0 0.0 0.0 86-87 0.38749999999999996 0.0 0.0 0.0 0.0 88-89 0.48750000000000004 0.0 0.0 0.0 0.0 90-91 0.5874999999999999 0.0 0.0 0.0 0.0 92-93 0.7625 0.0 0.0 0.0 0.0 94-95 0.9125 0.0 0.0 0.0 0.0 96-97 1.1 0.0 0.0 0.0 0.0 98-99 1.1749999999999998 0.0 0.0 0.0 0.0 100-101 1.3125 0.0 0.0 0.0 0.0 102-103 1.55 0.0 0.0 0.0 0.0 104-105 1.775 0.0 0.0 0.0 0.0 106-107 2.05 0.0 0.0 0.0 0.0 108-109 2.3499999999999996 0.0 0.0 0.0 0.0 110-111 2.725 0.0 0.0 0.0 0.0 112-113 3.0875 0.0 0.0 0.0 0.0 114-115 3.3875 0.0 0.0 0.0 0.0 116-117 3.8125 0.0 0.0 0.0 0.0 118-119 4.300000000000001 0.0 0.0 0.0 0.0 120-121 4.7625 0.0 0.0 0.0 0.0 122-123 5.2875 0.0 0.0 0.0 0.0 124-125 5.887499999999999 0.0 0.0 0.0 0.0 126-127 6.612500000000001 0.0 0.0 0.0 0.0 128-129 7.25 0.0 0.0 0.0 0.0 130-131 7.925 0.0 0.0 0.0 0.0 132-133 8.600000000000001 0.0 0.0 0.0 0.0 134-135 9.325 0.0 0.0 0.0 0.0 136-137 9.975 0.0 0.0 0.0 0.0 138-139 10.725 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TTTGTCC 15 1.14231094E-4 144.9625 145 GTACAAG 20 3.0755144E-4 112.957794 1 CCCGGGA 20 3.5913987E-4 108.72187 9 TACAAGG 20 3.5913987E-4 108.72187 2 AGGCCCG 20 3.5913987E-4 108.72187 6 ACAAGGC 20 3.5913987E-4 108.72187 3 CAAGGCC 20 3.5913987E-4 108.72187 4 GCCCGGG 20 3.5913987E-4 108.72187 8 GGCCCGG 25 8.7222434E-4 86.97751 7 AAGGCCC 25 8.7222434E-4 86.97751 5 AACTGAG 20 0.005942617 28.992498 95-99 GCAGCCT 20 0.005942617 28.992498 80-84 TCATGCA 20 0.005942617 28.992498 65-69 GCTGACC 20 0.005942617 28.992498 35-39 CTAGCGA 20 0.005942617 28.992498 50-54 CTGCTTC 20 0.005942617 28.992498 60-64 TTTGGAG 20 0.005942617 28.992498 110-114 CAGGCGA 20 0.005942617 28.992498 70-74 CCGGGAA 20 0.005942617 28.992498 10-14 ATCGCGA 20 0.005942617 28.992498 135-139 >>END_MODULE SRR6941606 read2 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR6941606_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 33.16325 34.0 33.0 34.0 33.0 34.0 2 33.29175 34.0 33.0 34.0 33.0 34.0 3 33.31575 34.0 33.0 34.0 33.0 34.0 4 33.30475 34.0 33.0 34.0 33.0 34.0 5 33.25825 34.0 33.0 34.0 33.0 34.0 6 37.40525 38.0 38.0 38.0 38.0 38.0 7 37.4435 38.0 38.0 38.0 38.0 38.0 8 37.35175 38.0 38.0 38.0 38.0 38.0 9 37.32425 38.0 38.0 38.0 38.0 38.0 10-14 37.351200000000006 38.0 38.0 38.0 37.8 38.0 15-19 37.344049999999996 38.0 38.0 38.0 38.0 38.0 20-24 37.3836 38.0 38.0 38.0 38.0 38.0 25-29 37.2899 38.0 38.0 38.0 37.2 38.0 30-34 37.368550000000006 38.0 38.0 38.0 37.8 38.0 35-39 37.376450000000006 38.0 38.0 38.0 37.6 38.0 40-44 37.327 38.0 38.0 38.0 37.0 38.0 45-49 37.3663 38.0 38.0 38.0 37.4 38.0 50-54 37.2893 38.0 38.0 38.0 37.0 38.0 55-59 37.28505 38.0 38.0 38.0 37.0 38.0 60-64 37.2096 38.0 38.0 38.0 36.8 38.0 65-69 37.119550000000004 38.0 38.0 38.0 36.8 38.0 70-74 37.1246 38.0 38.0 38.0 36.8 38.0 75-79 37.102500000000006 38.0 38.0 38.0 37.0 38.0 80-84 37.02065 38.0 38.0 38.0 36.2 38.0 85-89 37.0914 38.0 38.0 38.0 36.4 38.0 90-94 37.01035 38.0 38.0 38.0 36.0 38.0 95-99 36.87735 38.0 38.0 38.0 35.4 38.0 100-104 36.581399999999995 38.0 38.0 38.0 35.0 38.0 105-109 36.389149999999994 38.0 38.0 38.0 34.0 38.0 110-114 35.95695 38.0 38.0 38.0 33.2 38.0 115-119 35.80875 38.0 37.8 38.0 32.2 38.0 120-124 35.83785 38.0 37.4 38.0 32.4 38.0 125-129 35.9918 38.0 38.0 38.0 33.0 38.0 130-134 35.93725 38.0 37.8 38.0 33.0 38.0 135-139 35.61 38.0 36.8 38.0 31.0 38.0 140-144 35.0736 38.0 36.0 38.0 31.0 38.0 145-149 34.12885 38.0 35.0 38.0 25.8 38.0 150-151 29.589624999999998 35.5 27.0 38.0 7.5 38.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 1.0 4 0.0 5 0.0 6 0.0 7 0.0 8 1.0 9 1.0 10 1.0 11 0.0 12 2.0 13 0.0 14 1.0 15 1.0 16 4.0 17 6.0 18 5.0 19 3.0 20 3.0 21 4.0 22 3.0 23 7.0 24 15.0 25 9.0 26 12.0 27 18.0 28 24.0 29 30.0 30 30.0 31 54.0 32 62.0 33 69.0 34 103.0 35 197.0 36 505.0 37 2826.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 44.925 18.575 10.775 25.724999999999998 2 31.324999999999996 17.9 31.05 19.725 3 23.275000000000002 21.425 33.725 21.575 4 26.150000000000002 32.0 23.599999999999998 18.25 5 29.049999999999997 32.875 20.0 18.075 6 23.674999999999997 34.0 22.8 19.525000000000002 7 20.875 19.2 38.775 21.15 8 23.849999999999998 21.65 26.974999999999998 27.525 9 26.375 22.175 26.625 24.825 10-14 27.615000000000002 25.185000000000002 24.825 22.375 15-19 26.995 25.28 25.655 22.07 20-24 27.43 24.955 26.0 21.615000000000002 25-29 27.375475095019002 25.71014202840568 25.01500300060012 21.899379875975196 30-34 27.151357567878392 25.90129506475324 25.53627681384069 21.411070553527676 35-39 27.077707770777078 26.17761776177618 24.937493749374937 21.807180718071805 40-44 26.919999999999998 25.924999999999997 25.314999999999998 21.84 45-49 26.926346317315865 27.236361818090906 24.591229561478073 21.246062303115156 50-54 27.060000000000002 25.39 25.595000000000002 21.955 55-59 26.790000000000003 25.795 25.729999999999997 21.685 60-64 26.421321066053306 25.261263063153155 26.446322316115804 21.871093554677735 65-69 27.70915637345602 25.648847327099066 24.58368755313297 22.058308746311948 70-74 27.371842960740185 25.661415353838457 25.30632658164541 21.660415103775943 75-79 27.66638331916596 24.331216560828043 25.6262813140657 22.376118805940298 80-84 27.384168918242768 25.102571800260183 26.298408886220354 21.214850395276695 85-89 27.46912036805521 25.523828574286146 24.46867030054508 22.538380757113565 90-94 27.345000000000002 25.69 25.324999999999996 21.64 95-99 27.279999999999998 24.7 25.790000000000003 22.23 100-104 26.619640802441342 26.539596778228024 25.26389514232828 21.57686727700235 105-109 28.592169820766998 23.901071392810653 26.29918894562932 21.20756984079303 110-114 26.921346067303364 26.046302315115753 25.331266563328164 21.701085054252715 115-119 26.631331566578332 26.17130856542827 25.556277813890695 21.641082054102707 120-124 27.08 27.150000000000002 23.200000000000003 22.57 125-129 26.687668766876687 26.292629262926294 24.56745674567457 22.452245224522454 130-134 27.56689172293073 26.07151787946987 24.66116529132283 21.70042510627657 135-139 26.852685268526855 26.422642264226422 24.98249824982498 21.74217421742174 140-144 28.470694138827767 25.32006401280256 24.9249849969994 21.284256851370273 145-149 28.11046075341438 25.469007954374906 25.298914402921607 21.121616889289108 150-151 28.594669002627953 25.003128519584532 25.52871980978601 20.873482668001504 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.5 7 0.5 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.5 21 1.0 22 0.5 23 0.5 24 3.0 25 5.5 26 5.0 27 7.0 28 9.5 29 17.5 30 20.5 31 18.5 32 22.5 33 26.0 34 34.5 35 51.5 36 66.0 37 84.5 38 102.5 39 119.5 40 136.0 41 123.0 42 125.0 43 157.0 44 168.0 45 138.0 46 119.5 47 114.0 48 94.0 49 93.0 50 100.5 51 108.0 52 101.5 53 134.0 54 206.0 55 252.0 56 240.0 57 188.0 58 159.5 59 166.0 60 142.5 61 88.0 62 67.5 63 46.5 64 26.5 65 20.5 66 13.0 67 15.5 68 18.0 69 10.5 70 8.0 71 5.0 72 2.5 73 3.0 74 2.5 75 4.0 76 3.0 77 1.0 78 1.5 79 1.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.02 30-34 0.005 35-39 0.01 40-44 0.0 45-49 0.005 50-54 0.0 55-59 0.0 60-64 0.005 65-69 0.015 70-74 0.025 75-79 0.005 80-84 0.06999999999999999 85-89 0.015 90-94 0.0 95-99 0.0 100-104 0.055 105-109 0.13 110-114 0.005 115-119 0.005 120-124 0.0 125-129 0.01 130-134 0.025 135-139 0.01 140-144 0.02 145-149 0.055 150-151 0.11249999999999999 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 68.925 #Duplication Level Percentage of deduplicated Percentage of total 1 79.65179542981502 54.900000000000006 2 10.845121508886471 14.95 3 4.642727602466449 9.6 4 2.140007254261879 5.8999999999999995 5 0.9793253536452665 3.375 6 0.4715270221254987 1.95 7 0.3627130939426913 1.7500000000000002 8 0.29017047515415306 1.6 9 0.14508523757707653 0.8999999999999999 >10 0.4715270221254987 5.075 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTTCGATCCTGGCTCAGGATGAACGCTGGCGGCATGCTTAACACATGCAA 33 0.8250000000000001 No Hit ATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAA 23 0.575 No Hit GTGACCAGGCCCGGGCGACTGTTTACCAAAAACACAGGTCTCCGCAAAGT 22 0.5499999999999999 No Hit CCTGGCTCAGGATGAACGCTGGCGGCATGCTTAACACATGCAAGTCGAAC 15 0.375 No Hit GGCTTGCGGTGGATACCTAGGCACCCAGAGACGAGGAAGGGCGTAGCAAG 15 0.375 No Hit CCTTACCAGGGCTTGACATGCCGCGAATCCTCTTGAAAGAGAGGGGTGCC 15 0.375 No Hit AGTGACCAGGCCCGGGCGACTGTTTACCAAAAACACAGGTCTCCGCAAAG 14 0.35000000000000003 No Hit ATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCA 12 0.3 No Hit GTGGGAACTTAGTTTCCGTTTGGGTATGCGCCCTTGGATTGCTGTTGCAT 12 0.3 No Hit CCTGCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGCAA 11 0.27499999999999997 No Hit GCACAACATCCTTATGCATCCATTTCACATGTTAGGTGTAGCTGGTGTTT 11 0.27499999999999997 No Hit GTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGATCTT 10 0.25 No Hit GCTTGTTATATGGGTCGTGAGTGGGAACTTAGTTTCCGTTTGGGTATGCG 10 0.25 No Hit ATTTGGTCAAGAGGAAGAGACTTATAATATTGTGGCTGCTCATGGTTATT 9 0.22499999999999998 No Hit CTATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAA 9 0.22499999999999998 No Hit GGAAGCTTCTCTGATGGTATGCCTTTAGGAATATCTGGTACTTTCAACTT 9 0.22499999999999998 No Hit GCTAACTCCAAAAACCCGTCCTCAGTTCGGATTGCAGGCTGCAACTCGCC 9 0.22499999999999998 No Hit ATCGGATTGCACTTTTACCCAATTTGGGAAGCTGCATCCGTTGATGAATG 8 0.2 No Hit GGAAGGCCTACGGGTCGTCAACTTCTTTTCTCGGAGAAGAAACAATGACG 8 0.2 No Hit CGGACATTGGTCCTCGAGTGCAAAGGCAGAAGGGAGCTTGACTGCAAGAC 8 0.2 No Hit CATCAACCGTGCTAATCTTGGTATGGAAGTAATGCACGAACGTAATGCTC 8 0.2 No Hit TCTATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGA 8 0.2 No Hit GTTCCGACCCGCACGAAAGGCGTAACGATCTGGGCACTGTCTCGGAGAGA 8 0.2 No Hit GTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGATCTTACCATGAC 8 0.2 No Hit CTGAAAATGAATCTGCTAATGAGGGTTACAAATTTGGTCAAGAGGAAGAG 8 0.2 No Hit AGCACAACATCCTTATGCATCCATTTCACATGTTAGGTGTAGCTGGTGTT 7 0.17500000000000002 No Hit GCTAATTGTTCTACACTTCTTACTTGGTGTAGCTTGTTATATGGGTCGTG 7 0.17500000000000002 No Hit CTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGAT 7 0.17500000000000002 No Hit GCTAGTTTCAACAACTCTCGTTCTTTACACTTCTTCTTGGCTGCTTGGCC 7 0.17500000000000002 No Hit GCTTGCGGTGGATACCTAGGCACCCAGAGACGAGGAAGGGCGTAGCAAGC 7 0.17500000000000002 No Hit GTTAAGCCGGAGGAAGGAGAGGATGAGGCCAAGTCATCATGCCCCTTATG 7 0.17500000000000002 No Hit GGGAACTTAGTTTCCGTTTGGGTATGCGCCCTTGGATTGCTGTTGCATAT 7 0.17500000000000002 No Hit ATTGTATTCCAGGCAGAGCACAACATCCTTATGCATCCATTTCACATGTT 7 0.17500000000000002 No Hit GGATGATCAGCCACACTGGGACTGAGACACGGCCCAGACTCCTACGGGAG 7 0.17500000000000002 No Hit TTCAGCTCCTGTTGCAGCTGCGACTGCTGTTTTCTTGATTTACCCTATTG 7 0.17500000000000002 No Hit CAGGGCTTGACATGCCGCGAATCCTCTTGAAAGAGAGGGGTGCCCTCGGG 6 0.15 No Hit GGCTGTCGTCAGCTCGTGCCGTAAGGTGTTGGGTTAAGTCTCGCAACGAG 6 0.15 No Hit GGCTGATCTTCCCCAAGAGTCCACATCGACGGGAAGGTTTGGCACCTCGA 6 0.15 No Hit GTGAGTGGGAACTTAGTTTCCGTTTGGGTATGCGCCCTTGGATTGCTGTT 6 0.15 No Hit GCGAAAGCCTGACGGAGCAATGCCGCGTGGAGGTGGAAGGCCTACGGGTC 6 0.15 No Hit GAGAAATCCGCCCAAGGAGGGGCTCGCGTCTGATTAGCTAGTTGGTGAGG 6 0.15 No Hit TGAACGCTGGCGGCATGCTTAACACATGCAAGTCGAACGGGAAGTGGTGT 6 0.15 No Hit CTGACACTGAGAGACGAAAGCTAGGGGAGCAAATGGGATTAGAGACCCCA 6 0.15 No Hit CTTACCAGGTCCAGACATAGCAAGGATTGACAGACTGAGAGCTCTTTCTT 6 0.15 No Hit GTTATTGTGAGAATTCTTAATTCAAGAGTTGTAAGGAGGGACTTATGTCA 6 0.15 No Hit GTCAAGGTCGCGTTATTAATACTTGGGCTGATATCATCAACCGTGCTAAT 6 0.15 No Hit CCTGAACAGACCGCCGGTGTTAAGCCGGAGGAAGGAGAGGATGAGGCCAA 6 0.15 No Hit TATCTATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTT 6 0.15 No Hit CCGCAACTTCTGTATTTATTATCGCCTTCATCGCAGCCCCTCCAGTAGAT 5 0.125 No Hit CGGACACAGGTGGTGCATGGCTGTCGTCAGCTCGTGCCGTAAGGTGTTGG 5 0.125 No Hit GGGTGAGTAACGCGTAAGAACCTGCCCTTGGGAGGGGAACAACAACTGGA 5 0.125 No Hit GGAATTTTCCGCAATGGGCGAAAGCCTGACGGAGCAATGCCGCGTGGAGG 5 0.125 No Hit CCTAAATGGATTCAATTTCAACCAATCTGTAGTTGATAGTCAAGGTCGCG 5 0.125 No Hit GGTGTTTTGATGATCCCTACCTTATTGACCGCAACTTCTGTATTTATTAT 5 0.125 No Hit TATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAA 5 0.125 No Hit CTTGCGGTGGATACCTAGGCACCCAGAGACGAGGAAGGGCGTAGCAAGCG 5 0.125 No Hit ATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATA 5 0.125 No Hit ATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCTAAATGG 5 0.125 No Hit GTTGATAGTCAAGGTCGCGTTATTAATACTTGGGCTGATATCATCAACCG 5 0.125 No Hit ACCAGGGCTTGACATGCCGCGAATCCTCTTGAAAGAGAGGGGTGCCCTCG 5 0.125 No Hit GGAATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCTAAA 5 0.125 No Hit GCTTGACATGCCGCGAATCCTCTTGAAAGAGAGGGGTGCCCTCGGGAACG 5 0.125 No Hit GTTGCATATTCAGCTCCTGTTGCAGCTGCGACTGCTGTTTTCTTGATTTA 5 0.125 No Hit ATTCAGTGCTATGCATGGTTCCTTGGTAACCTCTAGTTTGATCAGGGAAA 5 0.125 No Hit ATCCTGGCTCAGGATGAACGCTGGCGGCATGCTTAACACATGCAAGTCGA 5 0.125 No Hit CTCTGGTGCTATTATTCCTACTTCTGCGGCAATCGGATTGCACTTTTACC 5 0.125 No Hit CAATGGTGGTCCTTATGAGCTAATTGTTCTACACTTCTTACTTGGTGTAG 5 0.125 No Hit GTTTCTGGTTCTTTACTTTATGGAAACAATATTATCTCTGGTGCTATTAT 5 0.125 No Hit CCTAGGCACCCAGAGACGAGGAAGGGCGTAGCAAGCGACGAAATGCTTCG 5 0.125 No Hit GTTAGGTGTAGCTGGTGTTTTCGGCGGTTCCCTATTCAGTGCTATGCATG 5 0.125 No Hit GTGTTTTCGGCGGTTCCCTATTCAGTGCTATGCATGGTTCCTTGGTAACC 5 0.125 No Hit AAGGCTTGCGGTGGATACCTAGGCACCCAGAGACGAGGAAGGGCGTAGCA 5 0.125 No Hit GTACAAGCCTGTGGGGTCGCTTCTGCAACTGGATAACTAGCACTGAAAAT 5 0.125 No Hit GTTTGATCAGGGAAACTACTGAAAATGAATCTGCTAATGAGGGTTACAAA 5 0.125 No Hit GGCTTTTCAAGTCCGCCGTCAAATCCCAGGGCTCAACCCTGGACAGGCGG 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0125 0.0 0.0 0.0 0.0 60-61 0.025 0.0 0.0 0.0 0.0 62-63 0.025 0.0 0.0 0.0 0.0 64-65 0.025 0.0 0.0 0.0 0.0 66-67 0.025 0.0 0.0 0.0 0.0 68-69 0.025 0.0 0.0 0.0 0.0 70-71 0.025 0.0 0.0 0.0 0.0 72-73 0.05 0.0 0.0 0.0 0.0 74-75 0.1 0.0 0.0 0.0 0.0 76-77 0.1 0.0 0.0 0.0 0.0 78-79 0.1 0.0 0.0 0.0 0.0 80-81 0.1125 0.0 0.0 0.0 0.0 82-83 0.15 0.0 0.0 0.0 0.0 84-85 0.3125 0.0 0.0 0.0 0.0 86-87 0.36250000000000004 0.0 0.0 0.0 0.0 88-89 0.4625 0.0 0.0 0.0 0.0 90-91 0.5874999999999999 0.0 0.0 0.0 0.0 92-93 0.7625 0.0 0.0 0.0 0.0 94-95 0.9125 0.0 0.0 0.0 0.0 96-97 1.1 0.0 0.0 0.0 0.0 98-99 1.1749999999999998 0.0 0.0 0.0 0.0 100-101 1.3125 0.0 0.0 0.0 0.0 102-103 1.5625 0.0 0.0 0.0 0.0 104-105 1.8 0.0 0.0 0.0 0.0 106-107 2.075 0.0 0.0 0.0 0.0 108-109 2.375 0.0 0.0 0.0 0.0 110-111 2.75 0.0 0.0 0.0 0.0 112-113 3.1125 0.0 0.0 0.0 0.0 114-115 3.4124999999999996 0.0 0.0 0.0 0.0 116-117 3.8375 0.0 0.0 0.0 0.0 118-119 4.300000000000001 0.0 0.0 0.0 0.0 120-121 4.75 0.0 0.0 0.0 0.0 122-123 5.2625 0.0 0.0 0.0 0.0 124-125 5.887499999999999 0.0 0.0 0.0 0.0 126-127 6.612500000000001 0.0 0.0 0.0 0.0 128-129 7.25 0.0 0.0 0.0 0.0 130-131 7.925 0.0 0.0 0.0 0.0 132-133 8.625 0.0 0.0 0.0 0.0 134-135 9.350000000000001 0.0 0.0 0.0 0.0 136-137 10.0 0.0 0.0 0.0 0.0 138-139 10.7625 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATTATCT 10 0.006830828 145.0 1 >>END_MODULE Read 1065005 spots for SRR6941606.sra Written 1065005 spots for SRR6941606.sra Read 1065005 spots for SRR6941606.sra Written 1065005 spots for SRR6941606.sra Read 1065005 spots for SRR6941606.sra Written 1065005 spots for SRR6941606.sra Read 1065005 spots for SRR6941606.sra Written 1065005 spots for SRR6941606.sra Read 1065005 spots for SRR6941606.sra Written 1065005 spots for SRR6941606.sra Read 1065005 spots for SRR6941606.sra Written 1065005 spots for SRR6941606.sra Read 1065005 spots for SRR6941606.sra Written 1065005 spots for SRR6941606.sra Read 1065005 spots for SRR6941606.sra Written 1065005 spots for SRR6941606.sra Read 1065005 spots for SRR6941606.sra Written 1065005 spots for SRR6941606.sra Read 1065005 spots for SRR6941606.sra Written 1065005 spots for SRR6941606.sra Read 1065005 spots for SRR6941606.sra Written 1065005 spots for SRR6941606.sra Read 1065005 spots for SRR6941606.sra Written 1065005 spots for SRR6941606.sra Read 1065005 spots for SRR6941606.sra Written 1065005 spots for SRR6941606.sra Read 1065005 spots for SRR6941606.sra Written 1065005 spots for SRR6941606.sra Read 1065005 spots for SRR6941606.sra Written 1065005 spots for SRR6941606.sra Read 1065005 spots for SRR6941606.sra Written 1065005 spots for SRR6941606.sra Read 1065013 spots for SRR6941606.sra Written 1065013 spots for SRR6941606.sra Read 1065005 spots for SRR6941606.sra Written 1065005 spots for SRR6941606.sra Read 1065005 spots for SRR6941606.sra Written 1065005 spots for SRR6941606.sra Read 1065005 spots for SRR6941606.sra Written 1065005 spots for SRR6941606.sra SRR ids: ['SRR6941606.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_5auiczk0 SRR6941606.sra spots: 21300108 blocks: [[1, 1065005], [1065006, 2130010], [2130011, 3195015], [3195016, 4260020], [4260021, 5325025], [5325026, 6390030], [6390031, 7455035], [7455036, 8520040], [8520041, 9585045], [9585046, 10650050], [10650051, 11715055], [11715056, 12780060], [12780061, 13845065], [13845066, 14910070], [14910071, 15975075], [15975076, 17040080], [17040081, 18105085], [18105086, 19170090], [19170091, 20235095], [20235096, 21300108]] SRR6941606 file size 7196207 SRR6941606 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR6941606 SRR6941606_1.fastq SRR6941606_2.fastq Input file: SRR6941606_1.fastq Paired file: SRR6941606_2.fastq trimmed: SRR6941606-trimmed-pair1.fastq, SRR6941606-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Fri Dec 6 12:57:32 2024 >> started Fri Dec 6 12:57:58 2024 >> done (26.235s) 21300108 read pairs processed; of these: 9528 ( 0.04%) short read pairs filtered out after trimming by size control 6916 ( 0.03%) empty read pairs filtered out after trimming by size control 21283664 (99.92%) read pairs available; of these: 10241860 (48.12%) trimmed read pairs available after processing 11041804 (51.88%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 19 2 0.00% 20 1 0.00% 21 9 0.00% 22 3 0.00% 23 2 0.00% 24 6 0.00% 25 4 0.00% 26 4 0.00% 27 12 0.00% 28 4 0.00% 29 6 0.00% 30 7 0.00% 31 7 0.00% 32 10 0.00% 33 7 0.00% 34 10 0.00% 35 11 0.00% 36 7 0.00% 37 9 0.00% 38 7 0.00% 39 23 0.00% 40 25 0.00% 41 19 0.00% 42 23 0.00% 43 28 0.00% 44 35 0.00% 45 32 0.00% 46 31 0.00% 47 41 0.00% 48 45 0.00% 49 55 0.00% 50 66 0.00% 51 85 0.00% 52 131 0.00% 53 114 0.00% 54 133 0.00% 55 169 0.00% 56 168 0.00% 57 201 0.00% 58 260 0.00% 59 296 0.00% 60 332 0.00% 61 399 0.00% 62 479 0.00% 63 557 0.00% 64 652 0.00% 65 731 0.00% 66 820 0.00% 67 943 0.00% 68 1140 0.01% 69 1200 0.01% 70 1438 0.01% 71 1637 0.01% 72 2054 0.01% 73 2204 0.01% 74 2306 0.01% 75 2801 0.01% 76 3210 0.02% 77 3441 0.02% 78 3948 0.02% 79 4710 0.02% 80 5262 0.02% 81 5765 0.03% 82 6321 0.03% 83 7499 0.04% 84 8244 0.04% 85 10139 0.05% 86 10440 0.05% 87 11434 0.05% 88 13426 0.06% 89 13526 0.06% 90 14898 0.07% 91 15710 0.07% 92 18130 0.09% 93 18823 0.09% 94 19647 0.09% 95 22250 0.10% 96 22387 0.11% 97 24850 0.12% 98 25553 0.12% 99 27136 0.13% 100 28153 0.13% 101 31178 0.15% 102 31310 0.15% 103 31745 0.15% 104 34097 0.16% 105 35074 0.16% 106 36493 0.17% 107 38601 0.18% 108 42184 0.20% 109 44000 0.21% 110 43560 0.20% 111 44445 0.21% 112 46400 0.22% 113 45718 0.21% 114 49917 0.23% 115 52560 0.25% 116 54418 0.26% 117 53735 0.25% 118 55636 0.26% 119 54787 0.26% 120 59120 0.28% 121 61845 0.29% 122 65767 0.31% 123 68425 0.32% 124 67707 0.32% 125 72675 0.34% 126 70856 0.33% 127 73091 0.34% 128 72845 0.34% 129 78155 0.37% 130 74753 0.35% 131 80592 0.38% 132 83576 0.39% 133 82169 0.39% 134 87008 0.41% 135 88003 0.41% 136 93308 0.44% 137 93062 0.44% 138 101032 0.47% 139 107272 0.50% 140 110516 0.52% 141 126467 0.59% 142 129612 0.61% 143 142580 0.67% 144 162724 0.76% 145 193861 0.91% 146 228568 1.07% 147 296764 1.39% 148 434544 2.04% 149 839794 3.95% 150 4802608 22.56% 151 11041804 51.88% 21283664 reads passed initial QC criterion=sequence-density sequence-density=1.04 sequence-density-rank=1 fanout-score=2.49 fanout-score-rank=26 prefix-density=1.07 prefix-fanout=2.4 sequence=GTTACGGCCGCCGTTCACCGGGGCTTCGGTCGCCGGCTTCCCTGTCATCAGTTCACCAACTTCCTTGACCTTCCGGCACTGGGCAGGCGTCAGCCCCCATACATGGTCTTACGACTTTGCGGAGACCTGTGTTTTTGGTAAACAGTCGCCCGGGCCTGGTCACTGCGACCCCCTTTTGTGAGGGGGCACCCCTTCT criterion=fanout-score sequence-density=0.01 sequence-density-rank=43 fanout-score=84.38 fanout-score-rank=1 prefix-density=0.20 prefix-fanout=2.5 sequence=CGACTTCACCCCAGTCGAAGACCCCACCGTGGTATGCGCCAATAAGACCACCAAAGGCCTTTGTGGCACTAGTGGTACACAGAAGTCATGGGTGATCATTGGTCCGATGCTTCGGGCGAAACCAATTCCCAGGGTGTGACGGGC criterion=sequence-density sequence-density=0.76 sequence-density-rank=1 fanout-score=2.34 fanout-score-rank=30 prefix-density=0.74 prefix-fanout=2.3 sequence=ATAACGGTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGACCCGCACGAAAGGCGTAACGATCTGGGCACTGTCTCGGAGAGAGGCTCGGTGAAATAGACATGTCTGTGAAGATGCGGACTACCTGCACCTGGACAGAAAGACCCTATGAAGCTTTACTGTTCCCTGGGATTGGCTTTGGGCCTTTCCTGCGCAGCTTAGGTGGAAGGCGAAGAAGGCCCCCTTCCGGGGGGGCCCGAGCCATCAGTGAGATACCACTCTGGAAGAGCTCGGATTCTAACCTTGTGTCAGACCCGCGGGCCAAGGGACAGTCTCAGGTAGACAGTTTCTATGGGGCGTAGGCCTCCCAAAAGGTAACGGAGGCGTGCAAAGGTTTCCTCGGGCCAGACGGACATTGGTCCTCGAGTGCAAAGGCAGAAGGGAGCTTGACTGCAAGACTCACCCGTCGAGCAGAGACGAAAGTCGGCCTTAGTGATCCGACGGTGCCGAGTGGAAGGGCCGTCGCTC criterion=fanout-score sequence-density=0.02 sequence-density-rank=33 fanout-score=70.77 fanout-score-rank=1 prefix-density=1.02 prefix-fanout=1.1 sequence=AGAAGGGGTGCCCCCTCACAAAAGGGGGTCGCAGTGACCAGGCCCGGGCGACTGTTTACCAAAAACACAGGTCTCCGCAAAGTCGTAAGACCATGTATGGGGGCTGACGCCTGCCCAGTGCCGGAAGGTCAAGGAAGTTGGTGAACTGATGACAGGGAAGCCGGCGACCGAAGCCCCGGTGAACGGCGGCCGTAAC SRR6941606 testing PE reads STAR mapping to Ensembl genome Started job on | Dec 06 12:58:43 Started mapping on | Dec 06 12:58:43 Finished on | Dec 06 13:00:22 Mapping speed, Million of reads per hour | 773.95 Number of input reads | 21283664 Average input read length | 292 UNIQUE READS: Uniquely mapped reads number | 10379030 Uniquely mapped reads % | 48.77% Average mapped length | 294.95 Number of splices: Total | 1418460 Number of splices: Annotated (sjdb) | 1261360 Number of splices: GT/AG | 1338358 Number of splices: GC/AG | 16608 Number of splices: AT/AC | 4627 Number of splices: Non-canonical | 58867 Mismatch rate per base, % | 0.16% Deletion rate per base | 0.01% Deletion average length | 1.55 Insertion rate per base | 0.01% Insertion average length | 1.74 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 7964245 % of reads mapped to multiple loci | 37.42% Number of reads mapped to too many loci | 369205 % of reads mapped to too many loci | 1.73% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 3.04% % of reads unmapped: other | 9.04% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 2945775 2945775 2945775 N_multimapping 7964245 7964245 7964245 N_noFeature 6230001 10128555 6334338 N_ambiguous 294348 5131 151891 UnstrandedReadsAssigned:3854681 PositiveStrandReadsAssigned:245344 NegativeStrandReadsAssigned:3892801 Dataset is classified negative stranded MeadianReadLen=151 20thPercentileLength=150 echo kmer=145 SRR6941606 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,972 [index] number of k-mers: 66,720,672 [index] number of equivalence classes: 111,837 [quant] running in paired-end mode [quant] will process pair 1: SRR6941606-trimmed-pair1.fastq SRR6941606-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 21,283,664 reads, 7,630,341 reads pseudoaligned [quant] estimated average fragment length: 215.595 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 959 rounds 52973 SRR6941606.ke.tsv 35125 SRR6941606.se.tsv 88098 total ==> SRR6941606.ke.tsv <== target_id length eff_length est_counts tpm PNS24245 936 721.919 0 0 PNS24247 1044 829.405 4.4259 0.414954 PNS24249 1928 1713.4 2.72229 0.123549 PNS24246 1044 829.405 4.4259 0.414954 PNS24248 1044 829.405 4.4259 0.414954 PNS24244 1471 1256.4 0 0 PNS24243 293 112.103 0 0 KQK14069 1603 1388.4 692.143 38.7655 KQK14071 474 269.144 16.7211 4.83108 ==> SRR6941606.se.tsv <== BRADI_1g14170v3 885 BRADI_1g53295v3 4 BRADI_1g59795v3 10 BRADI_1g07683v3 0 BRADI_1g00485v3 0 BRADI_1g20270v3 16 BRADI_1g74790v3 8 BRADI_1g09890v3 0 BRADI_1g77505v3 17 BRADI_1g48960v3 0 SRR6941606 completed mapping pipeline successfully