Starting /dee2/code/volunteer_pipeline.sh SRR6941607 current disk space = 1551109193728 free memory = 1604065668 SRR6941607 SRAfilesize 476d6d2b41565d3bfda430eb8c70f413 SRR6941607.sra SRR6941607.sra file validated SRR6941607 is paired end SRR6941607 is conventional basespace SRR6941607 read1 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR6941607_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.95725 34.0 33.0 34.0 32.0 34.0 2 33.22125 34.0 33.0 34.0 32.0 34.0 3 33.31875 34.0 33.0 34.0 32.0 34.0 4 33.4385 34.0 33.0 34.0 33.0 34.0 5 33.486 34.0 33.0 34.0 33.0 34.0 6 37.3035 38.0 38.0 38.0 36.0 38.0 7 37.669 38.0 38.0 38.0 38.0 38.0 8 37.685 38.0 38.0 38.0 38.0 38.0 9 37.69725 38.0 38.0 38.0 38.0 38.0 10-14 37.713049999999996 38.0 38.0 38.0 38.0 38.0 15-19 37.6913 38.0 38.0 38.0 38.0 38.0 20-24 37.6644 38.0 38.0 38.0 38.0 38.0 25-29 37.6674 38.0 38.0 38.0 38.0 38.0 30-34 37.540749999999996 38.0 38.0 38.0 38.0 38.0 35-39 37.5775 38.0 38.0 38.0 38.0 38.0 40-44 37.56305 38.0 38.0 38.0 38.0 38.0 45-49 37.595150000000004 38.0 38.0 38.0 38.0 38.0 50-54 37.546800000000005 38.0 38.0 38.0 38.0 38.0 55-59 37.5283 38.0 38.0 38.0 38.0 38.0 60-64 37.506949999999996 38.0 38.0 38.0 38.0 38.0 65-69 37.3904 38.0 38.0 38.0 37.4 38.0 70-74 37.425000000000004 38.0 38.0 38.0 37.0 38.0 75-79 37.45145 38.0 38.0 38.0 37.6 38.0 80-84 37.3474 38.0 38.0 38.0 37.2 38.0 85-89 37.19025 38.0 38.0 38.0 36.6 38.0 90-94 37.22195 38.0 38.0 38.0 36.6 38.0 95-99 37.139799999999994 38.0 38.0 38.0 36.0 38.0 100-104 37.173950000000005 38.0 38.0 38.0 36.0 38.0 105-109 37.01915 38.0 38.0 38.0 35.8 38.0 110-114 36.4842 38.0 38.0 38.0 34.4 38.0 115-119 36.47305 38.0 38.0 38.0 34.2 38.0 120-124 36.54255 38.0 38.0 38.0 34.2 38.0 125-129 36.641200000000005 38.0 38.0 38.0 34.8 38.0 130-134 36.4955 38.0 38.0 38.0 34.0 38.0 135-139 36.2598 38.0 38.0 38.0 33.4 38.0 140-144 36.0124 38.0 37.8 38.0 32.8 38.0 145-149 35.564 38.0 36.2 38.0 31.0 38.0 150-151 31.6075 35.5 31.0 38.0 16.5 38.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 1.0 8 0.0 9 0.0 10 0.0 11 0.0 12 1.0 13 0.0 14 1.0 15 0.0 16 1.0 17 1.0 18 0.0 19 3.0 20 2.0 21 1.0 22 1.0 23 2.0 24 2.0 25 6.0 26 8.0 27 11.0 28 10.0 29 14.0 30 25.0 31 32.0 32 46.0 33 62.0 34 91.0 35 191.0 36 401.0 37 3087.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 49.09377462568952 10.795902285263988 6.172839506172839 33.937483582873654 2 23.492619464598448 12.859644733550162 32.849637227920944 30.79809857393045 3 19.775000000000002 18.9 27.575 33.75 4 25.1 25.95 22.75 26.200000000000003 5 24.2 31.974999999999998 23.325000000000003 20.5 6 20.525 32.275 23.724999999999998 23.474999999999998 7 15.625 23.974999999999998 40.625 19.775000000000002 8 19.775000000000002 23.075000000000003 28.9 28.249999999999996 9 19.125 21.25 33.1 26.525 10-14 22.27 27.365000000000002 24.154999999999998 26.21 15-19 22.48 26.515 25.505 25.5 20-24 21.765 26.995 25.755 25.485000000000003 25-29 23.18 26.305 25.25 25.264999999999997 30-34 22.48 27.0 25.64 24.88 35-39 22.136106805340265 26.87634381719086 25.211260563028155 25.776288814440722 40-44 22.739095638255304 25.725290116046416 25.30012004801921 26.23549419767907 45-49 21.790000000000003 26.005 27.155 25.05 50-54 22.240008003601623 25.561502676204288 25.586513931269074 26.611975388925018 55-59 22.20833124968745 26.458968845326797 25.288793318997847 26.043906585987898 60-64 21.897189718971894 25.697569756975696 26.117611761176118 26.287628762876285 65-69 22.775000000000002 25.44 25.34 26.445 70-74 22.351117555877796 26.051302565128253 24.206210310515523 27.391369568478424 75-79 22.03 26.369999999999997 25.31 26.290000000000003 80-84 23.549999999999997 26.055 24.52 25.874999999999996 85-89 22.634999999999998 25.779999999999998 25.124999999999996 26.46 90-94 22.64613230661533 26.151307565378268 24.8012400620031 26.401320066003297 95-99 22.634999999999998 26.55 24.7 26.115 100-104 22.25001250562753 26.256815567005155 24.596068230703814 26.8971036966635 105-109 22.400000000000002 25.779999999999998 25.369999999999997 26.450000000000003 110-114 23.460513748554767 26.014175840748 25.31543759111245 25.209872819584778 115-119 23.003210917118203 26.67068031306442 24.32269717037929 26.003411599438092 120-124 22.849704616000803 26.174026234104335 23.72083708821468 27.255432061680185 125-129 23.044608921784356 27.000400080016 23.80476095219044 26.150230046009206 130-134 23.258488773315996 26.939040856128422 23.338500775116266 26.46396959543932 135-139 23.294999999999998 26.82 23.945 25.94 140-144 23.580000000000002 26.97 23.82 25.629999999999995 145-149 23.185 26.63 24.19 25.995 150-151 22.625 26.2625 23.8625 27.250000000000004 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 1.0 20 1.5 21 2.0 22 2.5 23 2.5 24 2.5 25 3.0 26 4.5 27 6.0 28 13.5 29 14.0 30 10.5 31 15.5 32 19.5 33 23.5 34 31.5 35 35.5 36 70.0 37 131.5 38 146.5 39 140.0 40 151.5 41 169.5 42 165.5 43 148.0 44 145.0 45 142.5 46 111.0 47 96.5 48 116.5 49 125.0 50 123.0 51 116.0 52 123.5 53 146.5 54 176.0 55 204.5 56 202.0 57 168.5 58 137.5 59 105.5 60 73.5 61 54.5 62 36.5 63 29.5 64 34.5 65 28.5 66 18.0 67 18.0 68 15.0 69 12.0 70 14.0 71 16.0 72 21.5 73 17.0 74 9.0 75 13.5 76 15.5 77 11.5 78 7.5 79 2.0 80 1.0 81 0.5 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 4.825 2 0.075 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.005 40-44 0.04 45-49 0.0 50-54 0.045 55-59 0.015 60-64 0.01 65-69 0.0 70-74 0.005 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.005 95-99 0.0 100-104 0.045 105-109 0.0 110-114 0.5349999999999999 115-119 0.33999999999999997 120-124 0.13 125-129 0.02 130-134 0.015 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 73.4 #Duplication Level Percentage of deduplicated Percentage of total 1 83.31062670299727 61.150000000000006 2 9.536784741144414 14.000000000000002 3 2.7247956403269753 6.0 4 1.9073569482288828 5.6000000000000005 5 1.2602179836512262 4.625 6 0.3746594005449591 1.6500000000000001 7 0.2724795640326975 1.4000000000000001 8 0.10217983651226158 0.6 9 0.13623978201634876 0.8999999999999999 >10 0.3746594005449591 4.075 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCATTAAT 25 0.625 No Hit GCTTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCA 18 0.44999999999999996 No Hit GTGCGACGTGGGGCTGGATCTCAGTGGATCGTGGCAGCAAGGCCACTCTG 17 0.42500000000000004 No Hit CTTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCAT 17 0.42500000000000004 No Hit GCGGAAAAACCTTATCCATTAATAGCGGGAACTTCAAGAGCAGCTAGATC 15 0.375 No Hit GCGGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTACG 13 0.325 No Hit GGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTACGTT 13 0.325 No Hit GTTGAAACTAGCATATTGGAAGATTAATCGGCCAAAATAACCATGAGCAG 13 0.325 No Hit GCCAAAATAACCATGAGCAGCCACAATATTATAAGTCTCTTCCTCTTGAC 12 0.3 No Hit CTTGTCCGTACCAGTTCTGAGTCGACTGTTCAGCGCTCGGGGAAAGCCCC 10 0.25 No Hit GTACAAGGCCCGGGAACGGATTCACCGCCGTATGGCTGACCGGCGATTAC 10 0.25 No Hit GGCCAAAATAACCATGAGCAGCCACAATATTATAAGTCTCTTCCTCTTGA 9 0.22499999999999998 No Hit GCCGAAAACACCAGCTACACCTAACATGTGAAATGGATGCATAAGGATGT 9 0.22499999999999998 No Hit GTTCGAGCTTTTCCTGGGAGTATGGCATCGGTTACATACTTCAGTGCCGT 9 0.22499999999999998 No Hit GCCAGCTCCTATAGTGTGACGGGCGGTGTGTACAAGGCCCGGGAACGGAT 9 0.22499999999999998 No Hit GGATGCATAAGGATGTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAA 8 0.2 No Hit TTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCATTAATA 8 0.2 No Hit GGTAAATCAAGAAAACAGCAGTCGCAGCTGCAACAGGAGCTGAATATGCA 8 0.2 No Hit CCCGAAGTTACGGGGCTATTTTGCCGAGTTCCTTAGAGAGAGTTGTCTCG 7 0.17500000000000002 No Hit GTGCAATCCGATTGCCGCAGAAGTAGGAATAATAGCACCAGAGATAATAT 7 0.17500000000000002 No Hit CGAGAGTTGTTGAAACTAGCATATTGGAAGATTAATCGGCCAAAATAACC 7 0.17500000000000002 No Hit GTCGGTTTCGGGTACAGGTACCCTTTTGTTGAAGGTCGTTCGAGCTTTTC 7 0.17500000000000002 No Hit GTGAGCATTACGTTCGTGCATTACTTCCATACCAAGATTAGCACGGTTGA 7 0.17500000000000002 No Hit CCCTCATTAGCAGATTCATTTTCAGTAGTTTCCCTGATCAAACTAGAGGT 7 0.17500000000000002 No Hit CGGAAAAACCTTATCCATTAATAGCGGGAACTTCAAGAGCAGCTAGATCT 7 0.17500000000000002 No Hit ATAACAAGCTACACCAAGTAAGAAGTGTAGAACAATTAGCTCATAAGGAC 7 0.17500000000000002 No Hit CGAGCTTTTCCTGGGAGTATGGCATCGGTTACATACTTCAGTGCCGTAGC 6 0.15 No Hit GTCGCAGCTGCAACAGGAGCTGAATATGCAACAGCAATCCAAGGGCGCAT 6 0.15 No Hit GCTGAATATGCAACAGCAATCCAAGGGCGCATACCCAAACGGAAACTAAG 6 0.15 No Hit GGGAAGTTGTGAGCATTACGTTCGTGCATTACTTCCATACCAAGATTAGC 6 0.15 No Hit GTCGCCCAGGGCATAAGGGGCATGATGACTTGGCCTCATCCTCTCCTTCC 6 0.15 No Hit GTGCTCTGCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTCCTA 6 0.15 No Hit GTATTCTCTACCTACCCACCTGTGTCGGTTTCGGGTACAGGTACCCTTTT 6 0.15 No Hit CTCAGAGCCAATCCTTTTCCCGAAGTTACGGATCCGTTTTGCCGACTTCC 6 0.15 No Hit GGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTACGTTC 6 0.15 No Hit CCTGTGTCGGTTTCGGGTACAGGTACCCTTTTGTTGAAGGTCGTTCGAGC 6 0.15 No Hit CTTTCTTTTCCTCTGGCTACTAAGATGTTTCAGTTCGCCAGGTTGTCTCT 6 0.15 No Hit GCGGTCAATAAGGTAGGGATCATCAAAACACCGAACCATCCGATGTAAAG 5 0.125 No Hit CTCCACCGCTTGTGCGGGCCCCCGTCAATTCCTTTGAGTTTCATTCTTGC 5 0.125 No Hit CGCAGAAGTAGGAATAATAGCACCAGAGATAATATTGTTTCCATAAAGTA 5 0.125 No Hit GTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTC 5 0.125 No Hit CAGAGAAGCTTCCTTGACCAATAGGGTAAATCAAGAAAACAGCAGTCGCA 5 0.125 No Hit GTCATTGTTTCTTCTCCGAGAAAAGAAGTTGACGACCCGTAGGCCTTCCA 5 0.125 No Hit GGAAAAACCTTATCCATTAATAGCGGGAACTTCAAGAGCAGCTAGATCTA 5 0.125 No Hit GTCGGATGGGGAGCCCGCAGGCCGTTGCAGCGCAGTGCCCCGAGGGACAC 5 0.125 No Hit GCTTTACCTGATAGAACTCGTAATGGGCTCCAGCTATCCTGAGGGAAACT 5 0.125 No Hit CAGATATTCCTAAAGGCATACCATCAGAGAAGCTTCCTTGACCAATAGGG 5 0.125 No Hit GTCTCTTCCTCTTGACCAAATTTGTAACCCTCATTAGCAGATTCATTTTC 5 0.125 No Hit CAAGTATTAATAACGCGACCTTGACTATCAACTACAGATTGGTTGAAATT 5 0.125 No Hit CGGGCGGTGTGTACAAGGCCCGGGAACGGATTCACCGCCGTATGGCTGAC 5 0.125 No Hit CCATATAACAAGCTACACCAAGTAAGAAGTGTAGAACAATTAGCTCATAA 5 0.125 No Hit GTCGCAAGCCTAGCCTTAGGCATCCCCCTCCTTACGGTTAAGGGTAATGA 5 0.125 No Hit TTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCATTA 5 0.125 No Hit GGCATACCATCAGAGAAGCTTCCTTGACCAATAGGGTAAATCAAGAAAAC 5 0.125 No Hit GCCACCTACAGACGCTTTACGCCCAATCATTCCGGATAACGCTTGCATCC 5 0.125 No Hit CCAGATTCCTACTACAGGCCAAGCAGCCAAGAAGAAGTGTAAAGAACGAG 5 0.125 No Hit GGATCATCAAAACACCGAACCATCCGATGTAAAGACGATTTTCAGTGCTA 5 0.125 No Hit AGGAATAATAGCACCAGAGATAATATTGTTTCCATAAAGTAAAGAACCAG 5 0.125 No Hit GCCGAGTTCCTTAGAGAGAGTTGTCTCGCGCCCCTAGGTATTCTCTACCT 5 0.125 No Hit GTGTGTACAAGGCCCGGGAACGGATTCACCGCCGTATGGCTGACCGGCGA 5 0.125 No Hit GCTGCGATGAAGGCGATAATAAATACAGAAGTTGCGGTCAATAAGGTAGG 5 0.125 No Hit GGATGTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAAAGTACCAGAT 5 0.125 No Hit CCTAGGTATTCTCTACCTACCCACCTGTGTCGGTTTCGGGTACAGGTACC 5 0.125 No Hit GCTAATCAGACGCGAGCCCCTCCTTGGGCGGATTTCTCCTTTTGCTCCTC 5 0.125 No Hit CATCAGAGAAGCTTCCTTGACCAATAGGGTAAATCAAGAAAACAGCAGTC 5 0.125 No Hit CTACAGGCCAAGCAGCCAAGAAGAAGTGTAAAGAACGAGAGTTGTTGAAA 5 0.125 No Hit GCCGTGTCTCAGTCCCAGTGTGGCTGATCATCCTCTCGGACCAGCTACTG 5 0.125 No Hit GTTAGCGGAAAAACCTTATCCATTAATAGCGGGAACTTCAAGAGCAGCTA 5 0.125 No Hit CTCATAAGGACCACCATTGTATAACCATTCATCAACGGATGCAGCTTCCC 5 0.125 No Hit GCATAAGGATGTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAAAGTA 5 0.125 No Hit GATGCATAAGGATGTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAAA 5 0.125 No Hit GTGAAATGGATGCATAAGGATGTTGTGCTCTGCCTGGAATACAATCATAA 5 0.125 No Hit GCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTCCTAAAGGCAT 5 0.125 No Hit GCCAAGAAGAAGTGTAAAGAACGAGAGTTGTTGAAACTAGCATATTGGAA 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.025 0.0 0.0 0.0 0.0 58-59 0.025 0.0 0.0 0.0 0.0 60-61 0.025 0.0 0.0 0.0 0.0 62-63 0.025 0.0 0.0 0.0 0.0 64-65 0.025 0.0 0.0 0.0 0.0 66-67 0.0625 0.0 0.0 0.0 0.0 68-69 0.075 0.0 0.0 0.0 0.0 70-71 0.075 0.0 0.0 0.0 0.0 72-73 0.15000000000000002 0.0 0.0 0.0 0.0 74-75 0.2625 0.0 0.0 0.0 0.0 76-77 0.3125 0.0 0.0 0.0 0.0 78-79 0.35 0.0 0.0 0.0 0.0 80-81 0.42500000000000004 0.0 0.0 0.0 0.0 82-83 0.5375000000000001 0.0 0.0 0.0 0.0 84-85 0.6125 0.0 0.0 0.0 0.0 86-87 0.8 0.0 0.0 0.0 0.0 88-89 1.05 0.0 0.0 0.0 0.0 90-91 1.25 0.0 0.0 0.0 0.0 92-93 1.4 0.0 0.0 0.0 0.0 94-95 1.7125 0.0 0.0 0.0 0.0 96-97 2.2 0.0 0.0 0.0 0.0 98-99 2.5875 0.0 0.0 0.0 0.0 100-101 2.9875 0.0 0.0 0.0 0.0 102-103 3.1875 0.0 0.0 0.0 0.0 104-105 3.5875000000000004 0.0 0.0 0.0 0.0 106-107 3.8625 0.0 0.0 0.0 0.0 108-109 4.2125 0.0 0.0 0.0 0.0 110-111 4.675000000000001 0.0 0.0 0.0 0.0 112-113 5.1375 0.0 0.0 0.0 0.0 114-115 5.487500000000001 0.0 0.0 0.0 0.0 116-117 5.824999999999999 0.0 0.0 0.0 0.0 118-119 6.4 0.0 0.0 0.0 0.0 120-121 7.0125 0.0 0.0 0.0 0.0 122-123 7.475 0.0 0.0 0.0 0.0 124-125 8.275 0.0 0.0 0.0 0.0 126-127 8.925 0.0 0.0 0.0 0.0 128-129 9.7125 0.0 0.0 0.0 0.0 130-131 10.4875 0.0 0.0 0.0 0.0 132-133 11.2625 0.0 0.0 0.0 0.0 134-135 12.075 0.0 0.0 0.0 0.0 136-137 12.9375 0.0 0.0 0.0 0.0 138-139 13.65 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCTGAAC 65 0.007814131 13.338462 140-144 >>END_MODULE SRR6941607 read2 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR6941607_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 33.26175 34.0 33.0 34.0 33.0 34.0 2 33.305 34.0 33.0 34.0 33.0 34.0 3 33.331 34.0 33.0 34.0 33.0 34.0 4 33.29825 34.0 33.0 34.0 33.0 34.0 5 33.3355 34.0 33.0 34.0 33.0 34.0 6 37.512 38.0 38.0 38.0 38.0 38.0 7 37.516 38.0 38.0 38.0 38.0 38.0 8 37.462 38.0 38.0 38.0 38.0 38.0 9 37.442 38.0 38.0 38.0 38.0 38.0 10-14 37.462900000000005 38.0 38.0 38.0 38.0 38.0 15-19 37.456900000000005 38.0 38.0 38.0 38.0 38.0 20-24 37.431599999999996 38.0 38.0 38.0 38.0 38.0 25-29 37.3922 38.0 38.0 38.0 38.0 38.0 30-34 37.4534 38.0 38.0 38.0 38.0 38.0 35-39 37.444500000000005 38.0 38.0 38.0 38.0 38.0 40-44 37.428599999999996 38.0 38.0 38.0 38.0 38.0 45-49 37.37945 38.0 38.0 38.0 38.0 38.0 50-54 37.3995 38.0 38.0 38.0 38.0 38.0 55-59 37.36525 38.0 38.0 38.0 38.0 38.0 60-64 37.269999999999996 38.0 38.0 38.0 37.2 38.0 65-69 37.2673 38.0 38.0 38.0 37.2 38.0 70-74 37.23760000000001 38.0 38.0 38.0 37.0 38.0 75-79 37.186899999999994 38.0 38.0 38.0 37.0 38.0 80-84 37.05395 38.0 38.0 38.0 36.2 38.0 85-89 37.0866 38.0 38.0 38.0 36.6 38.0 90-94 37.0434 38.0 38.0 38.0 36.0 38.0 95-99 36.93755 38.0 38.0 38.0 36.0 38.0 100-104 36.683400000000006 38.0 38.0 38.0 35.0 38.0 105-109 36.5511 38.0 38.0 38.0 34.8 38.0 110-114 36.166900000000005 38.0 38.0 38.0 34.0 38.0 115-119 36.01604999999999 38.0 37.8 38.0 33.0 38.0 120-124 36.0909 38.0 37.8 38.0 33.6 38.0 125-129 36.138250000000006 38.0 38.0 38.0 33.6 38.0 130-134 36.0506 38.0 38.0 38.0 33.4 38.0 135-139 35.77405 38.0 37.4 38.0 31.4 38.0 140-144 35.29025 38.0 36.0 38.0 31.0 38.0 145-149 34.189750000000004 38.0 35.0 38.0 25.8 38.0 150-151 29.436374999999998 35.5 27.0 38.0 7.5 38.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 1.0 4 2.0 5 1.0 6 3.0 7 0.0 8 0.0 9 1.0 10 1.0 11 1.0 12 3.0 13 1.0 14 3.0 15 3.0 16 2.0 17 2.0 18 5.0 19 3.0 20 6.0 21 3.0 22 6.0 23 6.0 24 4.0 25 10.0 26 6.0 27 18.0 28 11.0 29 19.0 30 23.0 31 36.0 32 58.0 33 81.0 34 113.0 35 172.0 36 464.0 37 2932.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 47.275 19.15 10.475 23.1 2 29.849999999999998 19.125 31.55 19.475 3 23.225 20.849999999999998 35.449999999999996 20.474999999999998 4 25.825 30.475 23.9 19.8 5 29.75 31.15 20.65 18.45 6 24.175 34.55 21.925 19.35 7 21.725 19.525000000000002 36.199999999999996 22.55 8 23.925 23.400000000000002 26.400000000000002 26.275 9 25.575 22.275 28.075 24.075 10-14 26.905 24.72 26.135 22.24 15-19 26.615 24.805 26.295 22.285 20-24 27.13 24.095 27.025 21.75 25-29 26.965393078615723 25.49009801960392 25.09501900380076 22.449489897979596 30-34 26.511325566278316 25.116255812790637 27.416370818540926 20.95604780239012 35-39 25.61256125612561 25.797579757975797 26.48264826482648 22.107210721072107 40-44 26.200000000000003 25.755 26.21 21.834999999999997 45-49 26.005 25.580000000000002 26.06 22.355 50-54 27.334999999999997 25.005 26.07 21.59 55-59 26.61 25.535000000000004 25.515 22.34 60-64 26.506325316265812 24.856242812140607 26.08130406520326 22.556127806390318 65-69 27.045409081816363 25.35507101420284 25.33506701340268 22.264452890578116 70-74 26.890378075615125 25.270054010802163 25.905181036207242 21.934386877375474 75-79 27.346367318365917 24.036201810090503 25.966298314915747 22.651132556627832 80-84 26.65732726272077 24.90118577075099 26.43718416971031 22.00430279681793 85-89 27.290458091618326 25.54010802160432 24.63992798559712 22.529505901180237 90-94 26.295 25.045 25.775 22.884999999999998 95-99 26.950000000000003 24.27 26.0 22.78 100-104 26.19178630383673 25.916662498124154 26.196788554849682 21.694762643189435 105-109 27.93771591648726 23.64191658739298 26.61092474841035 21.809442747709408 110-114 27.486374318715935 24.971248562428123 25.911295564778236 21.631081554077706 115-119 27.005000000000003 26.064999999999998 25.11 21.82 120-124 27.015 26.200000000000003 24.625 22.16 125-129 27.02135106755338 26.45632281614081 24.661233061653082 21.861093054652734 130-134 27.168150445133538 26.472941882564772 24.84745423627088 21.51145343603081 135-139 27.77638881944097 25.27626381319066 25.641282064103205 21.30606530326516 140-144 28.432108027006752 25.41635408852213 25.506376594148538 20.64516129032258 145-149 28.452071242745646 25.195117070242144 25.475285171102662 20.877526515909544 150-151 28.623279098873596 25.594493116395494 24.90613266583229 20.876095118898625 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.5 2 0.5 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.5 14 0.5 15 0.0 16 0.0 17 0.5 18 0.5 19 1.0 20 1.0 21 1.5 22 2.0 23 1.0 24 1.5 25 3.0 26 6.5 27 8.0 28 11.0 29 16.5 30 15.5 31 17.0 32 20.5 33 21.0 34 32.0 35 48.5 36 73.0 37 102.5 38 134.5 39 147.5 40 161.5 41 167.0 42 145.5 43 158.5 44 169.0 45 143.0 46 129.5 47 114.0 48 96.0 49 108.5 50 108.5 51 113.0 52 105.0 53 111.5 54 160.0 55 166.0 56 165.5 57 161.0 58 134.0 59 124.0 60 100.0 61 64.0 62 50.0 63 51.5 64 51.5 65 39.5 66 26.5 67 29.0 68 32.5 69 17.5 70 14.5 71 19.0 72 17.0 73 18.5 74 17.5 75 12.0 76 11.0 77 10.0 78 5.5 79 3.0 80 0.0 81 0.5 82 0.5 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.02 30-34 0.005 35-39 0.01 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.005 65-69 0.02 70-74 0.02 75-79 0.005 80-84 0.065 85-89 0.02 90-94 0.0 95-99 0.0 100-104 0.045 105-109 0.135 110-114 0.005 115-119 0.0 120-124 0.0 125-129 0.005 130-134 0.03 135-139 0.005 140-144 0.025 145-149 0.06 150-151 0.125 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 73.075 #Duplication Level Percentage of deduplicated Percentage of total 1 82.27848101265823 60.12499999999999 2 10.5029079712624 15.35 3 3.5579883681149505 7.8 4 1.163188504960657 3.4000000000000004 5 0.8210742387957578 3.0 6 0.4105371193978789 1.7999999999999998 7 0.5473828258638385 2.8000000000000003 8 0.13684570646595962 0.8 9 0.17105713308244952 1.125 >10 0.4105371193978789 3.8 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTTCGATCCTGGCTCAGGATGAACGCTGGCGGCATGCTTAACACATGCAA 22 0.5499999999999999 No Hit ATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAA 19 0.475 No Hit GCTTGTTATATGGGTCGTGAGTGGGAACTTAGTTTCCGTTTGGGTATGCG 14 0.35000000000000003 No Hit GCTGCGACTGCTGTTTTCTTGATTTACCCTATTGGTCAAGGAAGCTTCTC 12 0.3 No Hit CCTGGCTCAGGATGAACGCTGGCGGCATGCTTAACACATGCAAGTCGAAC 11 0.27499999999999997 No Hit AGGGAAACTACTGAAAATGAATCTGCTAATGAGGGTTACAAATTTGGTCA 11 0.27499999999999997 No Hit GTTAGGTGTAGCTGGTGTTTTCGGCGGTTCCCTATTCAGTGCTATGCATG 11 0.27499999999999997 No Hit GCACAACATCCTTATGCATCCATTTCACATGTTAGGTGTAGCTGGTGTTT 11 0.27499999999999997 No Hit CCTTACCAGGGCTTGACATGCCGCGAATCCTCTTGAAAGAGAGGGGTGCC 11 0.27499999999999997 No Hit ATCGGATTGCACTTTTACCCAATTTGGGAAGCTGCATCCGTTGATGAATG 10 0.25 No Hit GTTTTGATGATCCCTACCTTATTGACCGCAACTTCTGTATTTATTATCGC 10 0.25 No Hit GTTATACAATGGTGGTCCTTATGAGCTAATTGTTCTACACTTCTTACTTG 10 0.25 No Hit TATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAA 9 0.22499999999999998 No Hit GGGAAACTACTGAAAATGAATCTGCTAATGAGGGTTACAAATTTGGTCAA 9 0.22499999999999998 No Hit GTCTGACATGCGTGCGAGTCGACGGGTTCTGAAACCTGGGATGCGCAAGG 9 0.22499999999999998 No Hit GGCTTGCGGTGGATACCTAGGCACCCAGAGACGAGGAAGGGCGTAGCAAG 9 0.22499999999999998 No Hit GTTCAACCTAAATGGATTCAATTTCAACCAATCTGTAGTTGATAGTCAAG 9 0.22499999999999998 No Hit GGTCGCTTCTGCAACTGGATAACTAGCACTGAAAATCGTCTTTACATCGG 8 0.2 No Hit GCCAGTGGCGCGAAGCTACCGTGTGCCGGATTATGACTGAACGCCTCTAA 8 0.2 No Hit GCTAGTTTCAACAACTCTCGTTCTTTACACTTCTTCTTGGCTGCTTGGCC 8 0.2 No Hit TATCTATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTT 8 0.2 No Hit GTTTGGGAATGCAGCCCAAATCGGGCGGTAGACTCCGTCCAAGGCTAAAT 7 0.17500000000000002 No Hit GCTAATTGTTCTACACTTCTTACTTGGTGTAGCTTGTTATATGGGTCGTG 7 0.17500000000000002 No Hit GCCTGACGGAGCAATGCCGCGTGGAGGTGGAAGGCCTACGGGTCGTCAAC 7 0.17500000000000002 No Hit CTTTACTTTATGGAAACAATATTATCTCTGGTGCTATTATTCCTACTTCT 7 0.17500000000000002 No Hit CCTGCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGCAA 7 0.17500000000000002 No Hit GTTATTTTGGCCGATTAATCTTCCAATATGCTAGTTTCAACAACTCTCGT 7 0.17500000000000002 No Hit GGCGGGACTACCCGCTGAGTTTAAGCATATAAATAAGCGGAGGAGAAGAA 7 0.17500000000000002 No Hit ATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCTAAATGG 7 0.17500000000000002 No Hit GGAGTCTGACATGCGTGCGAGTCGACGGGTTCTGAAACCTGGGATGCGCA 7 0.17500000000000002 No Hit GTGGGAACTTAGTTTCCGTTTGGGTATGCGCCCTTGGATTGCTGTTGCAT 7 0.17500000000000002 No Hit GGGAACTTAGTTTCCGTTTGGGTATGCGCCCTTGGATTGCTGTTGCATAT 7 0.17500000000000002 No Hit GTTGGGTTAAGTCTCGCAACGAGCGCAACCCTCGTGTTTAGTTGCCACTA 7 0.17500000000000002 No Hit GTCAAGGTCGCGTTATTAATACTTGGGCTGATATCATCAACCGTGCTAAT 7 0.17500000000000002 No Hit GGGTAGAGAAAATGCCTCGAGCCGAGGTCCGAGTACCAAGCGCTGCAGCG 7 0.17500000000000002 No Hit GTTTGATCAGGGAAACTACTGAAAATGAATCTGCTAATGAGGGTTACAAA 7 0.17500000000000002 No Hit GCTAACTCCAAAAACCCGTCCTCAGTTCGGATTGCAGGCTGCAACTCGCC 7 0.17500000000000002 No Hit CTTTTACCCAATTTGGGAAGCTGCATCCGTTGATGAATGGTTATACAATG 6 0.15 No Hit GGCAAAACGGATCCGTAACTTCGGGAAAAGGATTGGCTCTGAGGACTGGG 6 0.15 No Hit GTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGATCTT 6 0.15 No Hit GGATGAACGCTGGCGGCATGCTTAACACATGCAAGTCGAACGGGAAGTGG 6 0.15 No Hit GGCTGACGCGGGCTTTGCTCGCTGATCCGATGATTCATGATAACTCGACG 6 0.15 No Hit CTATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAA 6 0.15 No Hit GTTCAGCCGGAGGTAGGGTCCAGTGGCCGGAAGAGCACCGCACGTCGCGC 6 0.15 No Hit CATCAACCGTGCTAATCTTGGTATGGAAGTAATGCACGAACGTAATGCTC 6 0.15 No Hit TGTAGCTGGTGTTTTCGGCGGTTCCCTATTCAGTGCTATGCATGGTTCCT 6 0.15 No Hit ATTATCTATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAAT 6 0.15 No Hit AGGAGTCTGACATGCGTGCGAGTCGACGGGTTCTGAAACCTGGGATGCGC 6 0.15 No Hit GAACAATGTAGGCAAGGGAAGTCGGCAAAACGGATCCGTAACTTCGGGAA 6 0.15 No Hit AGCACAACATCCTTATGCATCCATTTCACATGTTAGGTGTAGCTGGTGTT 5 0.125 No Hit CGGCGGTTCCCTATTCAGTGCTATGCATGGTTCCTTGGTAACCTCTAGTT 5 0.125 No Hit ATTTGGGAAGCTGCATCCGTTGATGAATGGTTATACAATGGTGGTCCTTA 5 0.125 No Hit ATTATTCCTACTTCTGCGGCAATCGGATTGCACTTTTACCCAATTTGGGA 5 0.125 No Hit TTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAA 5 0.125 No Hit GTCGCTTCTGCAACTGGATAACTAGCACTGAAAATCGTCTTTACATCGGA 5 0.125 No Hit CTGGTACTTTCAACTTTATGATTGTATTCCAGGCAGAGCACAACATCCTT 5 0.125 No Hit GAACCTTACCAGGGCTTGACATGCCGCGAATCCTCTTGAAAGAGAGGGGT 5 0.125 No Hit GGAAACTACTGAAAATGAATCTGCTAATGAGGGTTACAAATTTGGTCAAG 5 0.125 No Hit GCGTAGTCGATGGACAACAGGTCAATATTCCTGTACTACCCCTTGTTGGT 5 0.125 No Hit AGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGATCT 5 0.125 No Hit GCCGCGAATCCTCTTGAAAGAGAGGGGTGCCCTCGGGAACGCGGACACAG 5 0.125 No Hit AGAAAATGCCTCGAGCCGAGGTCCGAGTACCAAGCGCTGCAGCGCTGAAG 5 0.125 No Hit TCTAGTTTGATCAGGGAAACTACTGAAAATGAATCTGCTAATGAGGGTTA 5 0.125 No Hit GTAGCTTGTTATATGGGTCGTGAGTGGGAACTTAGTTTCCGTTTGGGTAT 5 0.125 No Hit GAGTCTGACATGCGTGCGAGTCGACGGGTTCTGAAACCTGGGATGCGCAA 5 0.125 No Hit ATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCA 5 0.125 No Hit GGGAAGTGGTGTTTCCAGTGGCGAACGGGTGAGTAACGCGTAAGAACCTG 5 0.125 No Hit TTTCAACCAATCTGTAGTTGATAGTCAAGGTCGCGTTATTAATACTTGGG 5 0.125 No Hit CAGTGACCAGGCCCGGGCGACTGTTTACCAAAAACACAGGTCTCCGCAAA 5 0.125 No Hit GCTGCATCCGTTGATGAATGGTTATACAATGGTGGTCCTTATGAGCTAAT 5 0.125 No Hit GGTACTTTCAACTTTATGATTGTATTCCAGGCAGAGCACAACATCCTTAT 5 0.125 No Hit GGCTTTTCAAGTCCGCCGTCAAATCCCAGGGCTCAACCCTGGACAGGCGG 5 0.125 No Hit GAGCACAACATCCTTATGCATCCATTTCACATGTTAGGTGTAGCTGGTGT 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.025 0.0 0.0 0.0 0.0 58-59 0.025 0.0 0.0 0.0 0.0 60-61 0.025 0.0 0.0 0.0 0.0 62-63 0.025 0.0 0.0 0.0 0.0 64-65 0.05 0.0 0.0 0.0 0.0 66-67 0.0875 0.0 0.0 0.0 0.0 68-69 0.1 0.0 0.0 0.0 0.0 70-71 0.1 0.0 0.0 0.0 0.0 72-73 0.175 0.0 0.0 0.0 0.0 74-75 0.2875 0.0 0.0 0.0 0.0 76-77 0.35 0.0 0.0 0.0 0.0 78-79 0.4 0.0 0.0 0.0 0.0 80-81 0.5 0.0 0.0 0.0 0.0 82-83 0.6375 0.0 0.0 0.0 0.0 84-85 0.7125 0.0 0.0 0.0 0.0 86-87 0.9 0.0 0.0 0.0 0.0 88-89 1.15 0.0 0.0 0.0 0.0 90-91 1.35 0.0 0.0 0.0 0.0 92-93 1.5125000000000002 0.0 0.0 0.0 0.0 94-95 1.8375 0.0 0.0 0.0 0.0 96-97 2.3 0.0 0.0 0.0 0.0 98-99 2.6875 0.0 0.0 0.0 0.0 100-101 3.0875 0.0 0.0 0.0 0.0 102-103 3.3 0.0 0.0 0.0 0.0 104-105 3.6875 0.0 0.0 0.0 0.0 106-107 3.9749999999999996 0.0 0.0 0.0 0.0 108-109 4.325 0.0 0.0 0.0 0.0 110-111 4.775 0.0 0.0 0.0 0.0 112-113 5.25 0.0 0.0 0.0 0.0 114-115 5.6375 0.0 0.0 0.0 0.0 116-117 5.975 0.0 0.0 0.0 0.0 118-119 6.55 0.0 0.0 0.0 0.0 120-121 7.1625 0.0 0.0 0.0 0.0 122-123 7.6375 0.0 0.0 0.0 0.0 124-125 8.412500000000001 0.0 0.0 0.0 0.0 126-127 9.05 0.0 0.0 0.0 0.0 128-129 9.8625 0.0 0.0 0.0 0.0 130-131 10.7125 0.0 0.0 0.0 0.0 132-133 11.4875 0.0 0.0 0.0 0.0 134-135 12.3 0.0 0.0 0.0 0.0 136-137 13.175 0.0 0.0 0.0 0.0 138-139 13.8875 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content pass >>END_MODULE Read 999509 spots for SRR6941607.sra Written 999509 spots for SRR6941607.sra Read 999509 spots for SRR6941607.sra Written 999509 spots for SRR6941607.sra Read 999509 spots for SRR6941607.sra Written 999509 spots for SRR6941607.sra Read 999509 spots for SRR6941607.sra Written 999509 spots for SRR6941607.sra Read 999509 spots for SRR6941607.sra Written 999509 spots for SRR6941607.sra Read 999509 spots for SRR6941607.sra Written 999509 spots for SRR6941607.sra Read 999509 spots for SRR6941607.sra Written 999509 spots for SRR6941607.sra Read 999509 spots for SRR6941607.sra Written 999509 spots for SRR6941607.sra Read 999509 spots for SRR6941607.sra Written 999509 spots for SRR6941607.sra Read 999509 spots for SRR6941607.sra Written 999509 spots for SRR6941607.sra Read 999509 spots for SRR6941607.sra Written 999509 spots for SRR6941607.sra Read 999509 spots for SRR6941607.sra Written 999509 spots for SRR6941607.sra Read 999509 spots for SRR6941607.sra Written 999509 spots for SRR6941607.sra Read 999509 spots for SRR6941607.sra Written 999509 spots for SRR6941607.sra Read 999509 spots for SRR6941607.sra Written 999509 spots for SRR6941607.sra Read 999509 spots for SRR6941607.sra Written 999509 spots for SRR6941607.sra Read 999511 spots for SRR6941607.sra Written 999511 spots for SRR6941607.sra Read 999509 spots for SRR6941607.sra Written 999509 spots for SRR6941607.sra Read 999509 spots for SRR6941607.sra Written 999509 spots for SRR6941607.sra Read 999509 spots for SRR6941607.sra Written 999509 spots for SRR6941607.sra SRR ids: ['SRR6941607.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_g0sm5wcn SRR6941607.sra spots: 19990182 blocks: [[1, 999509], [999510, 1999018], [1999019, 2998527], [2998528, 3998036], [3998037, 4997545], [4997546, 5997054], [5997055, 6996563], [6996564, 7996072], [7996073, 8995581], [8995582, 9995090], [9995091, 10994599], [10994600, 11994108], [11994109, 12993617], [12993618, 13993126], [13993127, 14992635], [14992636, 15992144], [15992145, 16991653], [16991654, 17991162], [17991163, 18990671], [18990672, 19990182]] SRR6941607 file size 6752316 SRR6941607 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR6941607 SRR6941607_1.fastq SRR6941607_2.fastq Input file: SRR6941607_1.fastq Paired file: SRR6941607_2.fastq trimmed: SRR6941607-trimmed-pair1.fastq, SRR6941607-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Fri Dec 6 13:01:52 2024 >> started Fri Dec 6 13:02:13 2024 >> done (20.946s) 19990182 read pairs processed; of these: 12865 ( 0.06%) short read pairs filtered out after trimming by size control 14141 ( 0.07%) empty read pairs filtered out after trimming by size control 19963176 (99.86%) read pairs available; of these: 10229541 (51.24%) trimmed read pairs available after processing 9733635 (48.76%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 4 0.00% 19 2 0.00% 20 4 0.00% 21 5 0.00% 22 4 0.00% 23 6 0.00% 24 8 0.00% 25 5 0.00% 26 10 0.00% 27 24 0.00% 28 11 0.00% 29 9 0.00% 30 13 0.00% 31 8 0.00% 32 15 0.00% 33 17 0.00% 34 16 0.00% 35 13 0.00% 36 16 0.00% 37 30 0.00% 38 27 0.00% 39 33 0.00% 40 43 0.00% 41 60 0.00% 42 47 0.00% 43 44 0.00% 44 67 0.00% 45 79 0.00% 46 87 0.00% 47 67 0.00% 48 116 0.00% 49 142 0.00% 50 179 0.00% 51 199 0.00% 52 256 0.00% 53 251 0.00% 54 326 0.00% 55 377 0.00% 56 378 0.00% 57 443 0.00% 58 498 0.00% 59 643 0.00% 60 709 0.00% 61 948 0.00% 62 1071 0.01% 63 1255 0.01% 64 1354 0.01% 65 1607 0.01% 66 1714 0.01% 67 1899 0.01% 68 2264 0.01% 69 2499 0.01% 70 2829 0.01% 71 3281 0.02% 72 4021 0.02% 73 4509 0.02% 74 4876 0.02% 75 5534 0.03% 76 6040 0.03% 77 6742 0.03% 78 7252 0.04% 79 8687 0.04% 80 9720 0.05% 81 10475 0.05% 82 11504 0.06% 83 13042 0.07% 84 14743 0.07% 85 16665 0.08% 86 17804 0.09% 87 19275 0.10% 88 21122 0.11% 89 21617 0.11% 90 23152 0.12% 91 24193 0.12% 92 27747 0.14% 93 28722 0.14% 94 29992 0.15% 95 33800 0.17% 96 33762 0.17% 97 35129 0.18% 98 35661 0.18% 99 36865 0.18% 100 39913 0.20% 101 41379 0.21% 102 43076 0.22% 103 43149 0.22% 104 46037 0.23% 105 46452 0.23% 106 47389 0.24% 107 48307 0.24% 108 50920 0.26% 109 54130 0.27% 110 54490 0.27% 111 55399 0.28% 112 56669 0.28% 113 55970 0.28% 114 62169 0.31% 115 64673 0.32% 116 66329 0.33% 117 63308 0.32% 118 63091 0.32% 119 64083 0.32% 120 67500 0.34% 121 70932 0.36% 122 76545 0.38% 123 77941 0.39% 124 77697 0.39% 125 81682 0.41% 126 81681 0.41% 127 83869 0.42% 128 81444 0.41% 129 85861 0.43% 130 83375 0.42% 131 87140 0.44% 132 89725 0.45% 133 89606 0.45% 134 97162 0.49% 135 96232 0.48% 136 99191 0.50% 137 101061 0.51% 138 109031 0.55% 139 110953 0.56% 140 111681 0.56% 141 124979 0.63% 142 127651 0.64% 143 137675 0.69% 144 155020 0.78% 145 178622 0.89% 146 207523 1.04% 147 262251 1.31% 148 376073 1.88% 149 726018 3.64% 150 4467824 22.38% 151 9733635 48.76% 19963176 reads passed initial QC criterion=sequence-density sequence-density=1.86 sequence-density-rank=1 fanout-score=2.10 fanout-score-rank=23 prefix-density=1.88 prefix-fanout=2.1 sequence=TTTCCTCTGGCT criterion=fanout-score sequence-density=0.01 sequence-density-rank=37 fanout-score=24.37 fanout-score-rank=1 prefix-density=0.12 prefix-fanout=1.7 sequence=GAACAAGGTTCAAATCGTTCGTTCGTTAGGATGCCTCAGCTGCATACATCACTGCACTTCCACTTGACACCTATTTAAACGGCTCGTCTCGCCGCTACCTTATCCTATTTCCATACTTCTGTCGCTCCATCCCCGTATGGGTGGAGAACCCGTCGCTGTCTCGGCTGTGATACCGGAGGCTCTAGGGAAGTCGGAGGAGAGAGCACTCATCTTGGGGTGGGCTTACTACTTATATGCTTTCAGCAGTTATCCTCTCCGCACTTGGCTACCCAGCGTTTACCGTAGGCACGATAACTGGTACACCAGAGGTGCGTCCTTCCCGGTCCTCTCGTACTAGGGAAAGGTCCTCTCAATGCTCTAACGCCCACACCGGATATGGACCGAACTGTCTCACGA criterion=sequence-density sequence-density=1.77 sequence-density-rank=1 fanout-score=2.01 fanout-score-rank=22 prefix-density=1.75 prefix-fanout=2.0 sequence=CAAGGCTAAATAC criterion=fanout-score sequence-density=0.04 sequence-density-rank=25 fanout-score=50.76 fanout-score-rank=1 prefix-density=1.86 prefix-fanout=1.1 sequence=AGAAGGGGTGCCCCCTCACAAAAGGGGGTCGCAGTGACCAGGCCCGGGCGACTGTTTACCAAAAACACAGGTCTCCGCAAAGTCGTAAGACCATGTATGGGGGCTGACGCCTGCCCAGTGCCGGAAGGTCAAGGAAGTTGGTGAACTGATGACAGGGAAGCCGGCGACCGAAGCCCCGGTGAACGGCGGCCGTAAC SRR6941607 testing PE reads STAR mapping to Ensembl genome Started job on | Dec 06 13:03:04 Started mapping on | Dec 06 13:03:04 Finished on | Dec 06 13:04:46 Mapping speed, Million of reads per hour | 704.58 Number of input reads | 19963176 Average input read length | 289 UNIQUE READS: Uniquely mapped reads number | 9086659 Uniquely mapped reads % | 45.52% Average mapped length | 292.81 Number of splices: Total | 1633764 Number of splices: Annotated (sjdb) | 1478856 Number of splices: GT/AG | 1558864 Number of splices: GC/AG | 18635 Number of splices: AT/AC | 5362 Number of splices: Non-canonical | 50903 Mismatch rate per base, % | 0.16% Deletion rate per base | 0.01% Deletion average length | 1.60 Insertion rate per base | 0.01% Insertion average length | 1.70 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 6898167 % of reads mapped to multiple loci | 34.55% Number of reads mapped to too many loci | 463452 % of reads mapped to too many loci | 2.32% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 3.63% % of reads unmapped: other | 13.97% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 3984581 3984581 3984581 N_multimapping 6898167 6898167 6898167 N_noFeature 4582407 8799841 4707883 N_ambiguous 311907 4986 153577 UnstrandedReadsAssigned:4192345 PositiveStrandReadsAssigned:281832 NegativeStrandReadsAssigned:4225199 Dataset is classified negative stranded MeadianReadLen=151 20thPercentileLength=146 echo kmer=141 SRR6941607 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,972 [index] number of k-mers: 66,720,672 [index] number of equivalence classes: 111,837 [quant] running in paired-end mode [quant] will process pair 1: SRR6941607-trimmed-pair1.fastq SRR6941607-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 19,963,176 reads, 7,536,063 reads pseudoaligned [quant] estimated average fragment length: 211.058 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,039 rounds 52973 SRR6941607.ke.tsv 35125 SRR6941607.se.tsv 88098 total ==> SRR6941607.ke.tsv <== target_id length eff_length est_counts tpm PNS24245 936 726.327 0 0 PNS24247 1044 833.942 5.25993 0.680335 PNS24249 1928 1717.94 8.2202 0.516122 PNS24246 1044 833.942 5.25993 0.680335 PNS24248 1044 833.942 5.25993 0.680335 PNS24244 1471 1260.94 0 0 PNS24243 293 114.413 0 0 KQK14069 1603 1392.94 852.705 66.0304 KQK14071 474 271.633 24.4135 9.69449 ==> SRR6941607.se.tsv <== BRADI_1g14170v3 966 BRADI_1g53295v3 19 BRADI_1g59795v3 22 BRADI_1g07683v3 0 BRADI_1g00485v3 0 BRADI_1g20270v3 19 BRADI_1g74790v3 18 BRADI_1g09890v3 0 BRADI_1g77505v3 23 BRADI_1g48960v3 0 SRR6941607 completed mapping pipeline successfully